CN109954009A - A kind of joint production process extracting SOD and general flavone from leaf of Moringa - Google Patents
A kind of joint production process extracting SOD and general flavone from leaf of Moringa Download PDFInfo
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- CN109954009A CN109954009A CN201711406004.XA CN201711406004A CN109954009A CN 109954009 A CN109954009 A CN 109954009A CN 201711406004 A CN201711406004 A CN 201711406004A CN 109954009 A CN109954009 A CN 109954009A
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- moringa
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- ethyl alcohol
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- 235000011347 Moringa oleifera Nutrition 0.000 title claims abstract description 33
- 241000220215 Moringa Species 0.000 title claims abstract description 29
- 229930003944 flavone Natural products 0.000 title claims abstract description 19
- 235000011949 flavones Nutrition 0.000 title claims abstract description 19
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 19
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title claims abstract description 14
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title claims abstract description 14
- 150000002212 flavone derivatives Chemical class 0.000 title claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 41
- 235000019441 ethanol Nutrition 0.000 claims abstract description 20
- 239000000706 filtrate Substances 0.000 claims abstract description 15
- 238000005119 centrifugation Methods 0.000 claims abstract description 9
- 238000000605 extraction Methods 0.000 claims abstract description 9
- 150000002213 flavones Chemical class 0.000 claims abstract description 5
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 5
- 244000179886 Moringa oleifera Species 0.000 claims abstract description 4
- 238000004108 freeze drying Methods 0.000 claims abstract description 4
- 238000003825 pressing Methods 0.000 claims abstract description 3
- 239000006228 supernatant Substances 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 239000013049 sediment Substances 0.000 claims description 5
- 239000002893 slag Substances 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 4
- 238000000703 high-speed centrifugation Methods 0.000 claims description 4
- 102000011759 adducin Human genes 0.000 claims description 3
- 108010076723 adducin Proteins 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 2
- 238000007605 air drying Methods 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 238000012545 processing Methods 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 4
- 238000001556 precipitation Methods 0.000 abstract description 3
- 238000012869 ethanol precipitation Methods 0.000 abstract description 2
- 238000001035 drying Methods 0.000 abstract 2
- 239000002699 waste material Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 5
- 239000000284 extract Substances 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 101710138460 Leaf protein Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical class [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- -1 flavone compound Chemical class 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 229940079877 pyrogallol Drugs 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y115/00—Oxidoreductases acting on superoxide as acceptor (1.15)
- C12Y115/01—Oxidoreductases acting on superoxide as acceptor (1.15) with NAD or NADP as acceptor (1.15.1)
- C12Y115/01001—Superoxide dismutase (1.15.1.1)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Alternative & Traditional Medicine (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses the joint production process that SOD and general flavone are extracted from leaf of Moringa.It is that filtrate and filter residue are obtained after drying, extraction, filters pressing using fresh leaf of Moringa as raw material, leaf of Moringa SOD is made in filtrate after centrifugation, fractional precipitation, freeze-drying；Filter residue produces total flavones of Moringa oleifera leaf through drying, the extraction of ultrasonic wave added ethyl alcohol.The present invention makes the joint production process of leaf of Moringa SOD and general flavone, realizes the comprehensive utilization and high-valued exploitation of leaf of Moringa using modern processings such as isoelectric precipitation, ethanol precipitation, ultrasound assisted extractions, without waste, it is pollution-free, it is with short production cycle, it is suitble to industrialized production.
Description
Technical field
The present invention relates to field of biotechnology, are related specifically to a kind of coproduction that SOD and general flavone are extracted from leaf of Moringa
Technique.
Background technique
Moringa is perennial woody plant, originates in north India Himalayas, is a kind of with very high edible
With the tropical plants of medical value.Numerous studies prove, leaf of Moringa protein rich in, general flavone and superoxides discrimination
Change multiple efficacies ingredients such as enzyme (SOD).Superoxide dismutase contained by leaf of Moringa (SOD), which has, to be removed free radical, adjusts first
Shape parathyrine and liver lipid peroxidation effect.The physiological activity of flavone compound is relatively broad, have remove free radical, it is anti-oxidant,
Anti-cancer and cancer-preventing, treatment cardiovascular disease, it is hypoglycemic the effects of, widely paid attention to by the world of medicine.In recent years, Moringa is wide in China
All widely introducing and planting, Related product are developed into hot spot on the ground such as east, Yunnan and Hainan.But the country is for Moringa
In terms of research is concentrated mainly on cultivation technique and Analysis of Nutritive Composition, so far there are no related extraction SOD synchronous from leaf of Moringa and
The patent report of general flavone technique.
Summary of the invention
The purpose of the present invention be based on above-mentioned condition and propose it is a kind of from leaf of Moringa extract SOD and general flavone connection
Production. art, the technique is using leaf of Moringa abundant as raw material, using modern processing, in the same technological process of production, and production
The SOD and flavones of high added value, high bioactivity are provided, simple process and low cost is easy to implement.
To achieve the above object, the present invention is realized using following technical scheme: one kind from leaf of Moringa extract SOD and
The joint production process of general flavone, processing step are as follows:
(1) it takes fresh leaf of Moringa to clean, is dried after air-drying in 50 DEG C, crushed 80 mesh, obtain dry leaf of Moringa powder, it is spare；
(2) dry leaf of Moringa powder is taken, 20 times of dry powder weight of deionized water is added, is stirred continuously at 50~55 DEG C, extraction 3 is small
When, filtrate and filter residue are collected in filters pressing respectively；
(3) filtrate obtained by step (2) is centrifuged at a high speed, is collected supernatant (I)；
(4) supernatant (I) obtained by step (3) is taken, its pH value is adjusted to 5.0~5.2, the pre-cooling of its volume 40-50% is added
95% ethyl alcohol, after stirring 3 minutes, after standing 30 minutes at 0-4 DEG C, high speed centrifugation is collected supernatant (II)；
(5) 95% ethyl alcohol for continuing addition pre-cooling into step (4) gained supernatant (II), until reaching initial supernatant liquid (I) body
Long-pending 1 times after stirring 3 minutes, stands 30 minutes in 0-4 DEG C, and high speed centrifugation collects sediment, then heavy with deionized water dissolving
Supernatant is collected in starch, centrifugation, and freeze-drying obtains SOD freeze-dried powder；
(6) filter residue obtained by step (2) is taken, after being dried to water content≤15%, adds 95% ethyl alcohol, in 70 DEG C of ultrasound assisted extractions two
Secondary, supersonic frequency 10-15kHz, wherein once adding 95% ethyl alcohol to extract 2 hours by slag liquid ratio 1:20, another time by slag liquid than 1:
10 add 95% ethyl alcohol to extract 1 hour, filter to get filtrate, and merging filtrate recycles ethyl alcohol, are concentrated under reduced pressure, the vacuum at 50 DEG C -60 DEG C
It is dried, crushed into powder, obtains total flavones of Moringa oleifera leaf powder.
Being centrifuged at a high speed in above-mentioned steps (3) is centrifuged using tube centrifuge, and revolving speed 15000r/min is centrifuged 30min.
Being centrifuged at a high speed in above-mentioned steps (4) is centrifuged using tube centrifuge, revolving speed 10000r/min, centrifugation
30min。
Being centrifuged at a high speed in above-mentioned steps (5) is centrifuged using tube centrifuge, revolving speed 10000r/min, centrifugation
30min。
The present invention has the advantages that
(1) present invention is using leaf of Moringa abundant as raw material, using modern processing, in the same technological process of production, formulates
The joint production process of combined extracting Moringa SOD and general flavone out, the joint production process have no that patent family is reported.
(2) present invention use isoelectric precipitation and ethanol precipitation method, by SOD from Moringa leaf protein separation and Extraction
Out, it without using toxic organic solvents such as chloroform, acetone, avoids toxic solvent in environmental pollution and product and remains, there is life
Produce the period it is short, it is at low cost, be suitble to industrial-scale production the characteristics of.
(3) present invention extracts Moringa general flavone using ultrasonic wave added ethanol immersion, and extraction efficiency is high, and ethyl alcohol is non-toxic
Easily recycling.
(4) present invention SOD obtained activity is high, and unit enzyme activity reaches 3000U/mg or more, can be used for health food,
Cosmetics and medical industry raw material.
Specific embodiment
Embodiment is enumerated below, and concrete operation step is described further:
(1) it weighs by dry, crush 10 kilograms of leaf of Moringa powder, 200 kilograms of deionized water is added, be warming up to 52 DEG C, protect
Temperature is stirred continuously extraction, by feed liquid through plate and frame filter press squeeze and filter (100 mesh of filter cloth, filter pressure after extraction 3 hours
0.15MPa), filtrate 167L is obtained, 25 kilograms of filter residue；
(3) filtrate obtained by step (1) is centrifuged 30min with the tube centrifuge of revolving speed 15000r/min, removes sediment, in collection
(I) 145 L of clear liquid；
(4) 95% pre-cooled ethyl alcohol of 65L is added to 5.2 in supernatant (I) pH value obtained by set-up procedure (3), after stirring 3 minutes,
After standing 30 minutes at 0-4 DEG C, 10000r/min is centrifuged 30min, collects supernatant (II)；
(5) continue to add 95% ethyl alcohol that 80L is pre-chilled into step (4) gained supernatant (II), after continuing stirring 3 minutes, in 0-
4 DEG C stand 30 minutes, and 10000r/min is centrifuged 30min, collect sediment, then with deionized water dissolving sediment, 3000r/min
Centrifugation 10 minutes, collects supernatant, and freeze-drying obtains SOD freeze-dried powder 1.32g.Through using micro pyrogallol method (325nm method)
Detection activity, SOD unit enzymatic activity are 3260U/mg；
(6) filter residue obtained by step (2) is dried, obtains 8.6 kilograms of dry slag, add 170 liter of 95% ethyl alcohol, in 70 DEG C of ultrasound assisted extraction 2
Hour, filter to get filtrate, filter residue adds 95% ethyl alcohol of 90L again, 70 DEG C ultrasound assisted extraction 1 hour, filter to get filtrate, merge two
Secondary filtrate recycles ethyl alcohol, is concentrated under reduced pressure, is dried in vacuo at 50 DEG C -60 DEG C, is ground into powder, obtain total flavones of Moringa oleifera leaf 416g,
The general flavone recovery rate of dry leaf of Moringa is 4.16%.
Claims (5)
1. a kind of joint production process for extracting SOD and general flavone from leaf of Moringa, it is characterised in that: comprise the following steps that:
(1) it takes fresh leaf of Moringa to clean, is dried after air-drying in 50 DEG C, crushed 80 mesh, obtain dry leaf of Moringa powder, it is spare；
(2) dry leaf of Moringa powder is taken, 20 times of dry powder weight of deionized water is added, is stirred continuously at 50~55 DEG C, extraction 3 is small
When, filtrate and filter residue are collected in filters pressing respectively；
(3) filtrate obtained by step (2) is centrifuged at a high speed, is collected supernatant (I)；
(4) supernatant (I) obtained by step (3) is taken, its pH value is adjusted to 5.0~5.2, the pre-cooling of its volume 40-50% is added
95% ethyl alcohol, after stirring 3 minutes, after standing 30 minutes at 0-4 DEG C, high speed centrifugation is collected supernatant (II)；
(5) 95% ethyl alcohol for continuing addition pre-cooling into step (4) gained supernatant (II), until reaching initial supernatant liquid (I) body
Long-pending 1 times after stirring 3 minutes, stands 30 minutes in 0-4 DEG C, and high speed centrifugation collects sediment, then heavy with deionized water dissolving
Supernatant is collected in starch, centrifugation, and freeze-drying obtains SOD freeze-dried powder；
(6) filter residue obtained by step (2) is taken, after being dried to water content≤15%, adds 95% ethyl alcohol, in 70 DEG C of ultrasound assisted extractions two
Secondary, supersonic frequency 10-15kHz, wherein once adding 95% ethyl alcohol to extract 2 hours by slag liquid ratio 1:20, another time by slag liquid than 1:
10 add 95% ethyl alcohol to extract 1 hour, filter to get filtrate, and merging filtrate recycles ethyl alcohol, are concentrated under reduced pressure, the vacuum at 50 DEG C -60 DEG C
It is dried, crushed into powder, obtains total flavones of Moringa oleifera leaf powder.
2. joint production process according to claim 1, it is characterised in that: being centrifuged at a high speed as using pipe in step (3)
The centrifugation of formula centrifuge, revolving speed 15000r/min are centrifuged 30min.
3. joint production process according to claim 1, it is characterised in that: being centrifuged at a high speed as using pipe in step (4)
The centrifugation of formula centrifuge, revolving speed 10000r/min are centrifuged 30min.
4. joint production process according to claim 1, it is characterised in that: being centrifuged at a high speed as using pipe in step (5)
The centrifugation of formula centrifuge, revolving speed 10000r/min are centrifuged 30min.
5. joint production process according to claim 1, it is characterised in that: fresh leaf of Moringa described in step (1) is 3 after picking
Moringa branches and leaves in hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711406004.XA CN109954009A (en) | 2017-12-22 | 2017-12-22 | A kind of joint production process extracting SOD and general flavone from leaf of Moringa |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711406004.XA CN109954009A (en) | 2017-12-22 | 2017-12-22 | A kind of joint production process extracting SOD and general flavone from leaf of Moringa |
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| Publication Number | Publication Date |
|---|---|
| CN109954009A true CN109954009A (en) | 2019-07-02 |
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ID=67019445
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711406004.XA Pending CN109954009A (en) | 2017-12-22 | 2017-12-22 | A kind of joint production process extracting SOD and general flavone from leaf of Moringa |
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| Country | Link |
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| CN (1) | CN109954009A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110721134A (en) * | 2019-11-29 | 2020-01-24 | 广州环亚化妆品科技有限公司 | Method for extracting tyrosinase inhibitor from moringa seeds, tyrosinase inhibitor prepared by same and application thereof |
| CN114149982A (en) * | 2021-12-03 | 2022-03-08 | 西安全奥生物科技有限公司 | Method for extracting fructus Rosae Normalis superoxide dismutase |
-
2017
- 2017-12-22 CN CN201711406004.XA patent/CN109954009A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110721134A (en) * | 2019-11-29 | 2020-01-24 | 广州环亚化妆品科技有限公司 | Method for extracting tyrosinase inhibitor from moringa seeds, tyrosinase inhibitor prepared by same and application thereof |
| CN114149982A (en) * | 2021-12-03 | 2022-03-08 | 西安全奥生物科技有限公司 | Method for extracting fructus Rosae Normalis superoxide dismutase |
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