CN1448410A - Wolfberry fruit polysaccharide crude product extracting and separating technology - Google Patents

Abstract

The wolfberry polysaccharide extracting and separating technology belongs to biologically pharmaceutical engineering. Fresh wolfberry fruit is beated, squeezed and separated to eliminate wolfberry seed and the obtain wolfberry juice is used as the material for producing wolfberry polysaccharide. In the membrane separating process, membrane in 500 KD molecular weight level is first used to eliminate great molecular weight pigment and insoluble solid matter; membrane in 100 KD molecular weight level is then used to intercept wolfberry juice with dissolved wolfberry polysaccharide; and the juice is vacuum freeze dried to obtain coarse wolfberry polysaccharide product. The said process of the present invention has high yield of wolfberry polysaccharide product with high wolfberry polysaccharide content and may utilize wolfberry fruit comprehensively.

Description

Wolfberry fruit polysaccharide crude product extracting and separating technology

Technical field: the invention belongs to the biotech medicine product field.

Background technology: Chinese Pharmacopoeia later version in 1977 stipulates that clearly medicinal matrimony vine is the dry mature fruit of lycium barbarum Lycium barbaum L..Generally cry ' wolfberry fruit '.Medical circle is by pharmacology and clinical and experimental study, the main biologically active substance that generally believes lycium barbarum is lycium barbarum polysaccharide (Lycium Barbarumpolysaccharida.LBP), it is a kind of nonspecific immunostimulant, energy enhance immunity function, strengthen resistance against diseases, thereby reach effects such as anti-ageing, anti-oxidant, anti-hepatitis, anticancer and anti-AIDS.Prior art generally selects for use wolfberry fruit to make raw material to the extraction of lycium barbarum polysaccharide (crude product), adopts ' the division precipitator method ' to extract, and that is to say and utilizes different solubility, adds ethanol adjusting different concns and divides precipitation, i.e. ' ethanol precipitation '.Its technical process is: wolfberry fruit → pressurized water lixiviate → centrifugal or squeezing separations → extracting solution are concentrated into proportion 1.12 → 50% alcohol alcohol analyses → separates → must on ask liquid → be concentrated into proportion 1.25 → 85% alcohol alcohol analyse → separate → precipitate → allocate to proportion 1.02～1.03 → ultrafiltration to proportion 1.05 → 85% alcohol alcohol analyse → separate → precipitate → vacuum-drying → pulverizing → mistake 80 mesh sieves → must lycium barbarum polysaccharide (crude product).Because the raw material that traditional technology adopts is dry wolfberry, the isolating bits in the leaching process can not be fully utilized; Lycium barbarum polysaccharide (crude product) yield that this method is extracted is low, and between 2%～5% (wolfberry fruit needs the fresh Chinese wolfberry fruit of 5kg to dry or airing becomes 1kg) usually, and complex technical process are difficult to control and produce.

Summary of the invention: the objective of the invention is to extract lycium barbarum polysaccharide (crude product) processing method and fall behind at prior art, weak points such as the lycium barbarum polysaccharide yield is low, provide a kind of suitability for industrialized production that realizes, processing method advanced person, the lycium barbarum polysaccharide that the lycium barbarum polysaccharide yield is high (crude product) extraction separation new technology.

The technical process of the technology of the present invention is such.Raw material does not adopt drying wolfberry fruit, but adopts fresh matrimony vine berry, and after the matrimony vine seed was removed in separation, the gained Fructus Lycii original juice was as the raw material that separates lycium barbarum polysaccharide (crude product).Adopt membrane separation technique: can directly carry out the isolating metallic membrane of thick substances with a kind of of 500,000 molecular weight earlier, separate wolfberry pigment and insoluble solid particulate; Also can filter out the wolfberry pigment of macromolecule with the hanging of the thick filter cloth of common 300 orders nature, fall insoluble solid with 500,000 common molecular weight membrane sepn again.Then, use the flat film of 100,000 molecular weight again, hold back the following Fructus Lycii original juice part of 100,000 molecular weight of dissolving lycium barbarum polysaccharide, get lycium barbarum polysaccharide (crude product) carrying out vacuum lyophilization.

1, the technological process of production of Fructus Lycii original juice is: the purify waste water drip washing → 5% potassium permanganate of the fresh fructus lycii berry → tap water flushing → cleaning machine of harvesting was soaked 5 minutes, oxidation is removed the pesticide residue → spray Cleaning for High Capacity of purifying waste water → making beating juice extractor and is squeezed the juice, go seed, skin → colloidal mill defibrination → plate-type heat exchanger temperature automatically controlled≤83 ℃ of sterilization → sterile packeds, refrigeration → Fructus Lycii original juice.

1.25t fresh matrimony vine berry can be produced the 1t Fructus Lycii original juice, yield 80%.Can isolate about matrimony vine seed 25kg yield about 2%.The different yield differences of matrimony vine kind; Autumn, fruit was than summer fruit yield height.

Isolated matrimony vine seed when 2, producing Fructus Lycii original juice can adopt supercritical CO ₂Extraction process prepares Boxthorn Seed Oil.Technical process: the matrimony vine seed is pulverized, and crosses the 40 mesh sieves → supercritical CO of packing into ₂Extraction kettle → control pressure 25 ± 1Mpa of extraction plant, 36 ℃～39 ℃ extractions of temperature → the first extraction-container control pressure, 8 ± 1Mpa, temperature is resolved → the second extraction-container control pressure 6Mpa, 24 ℃～32 ℃ parsing → Boxthorn Seed Oils of temperature for 30 ℃～34 ℃.7～8 hours extraction time; Yield 15%.The maximum characteristics of Boxthorn Seed Oil are the β-Hu Luobusus that contain 2600PPM.

1.25t the matrimony vine berry can separate the matrimony vine seed about 25kg, wherein 6kg matrimony vine seed is used as the carrier of extraction separation β-Hu Luobusu, and 19kg can extract the 2.85kg Boxthorn Seed Oil.

3, Fructus Lycii original juice uses 500,000 molecular weight membrane sepn to go out macromolecule wolfberry pigment and the thick thing of insoluble solid, becomes the wolfberry pigment powder through vacuum lyophilization, uses Boxthorn Seed Oil as carrier again, adopts supercritical CO ₂Extraction process extraction β-Hu Luobusu, its technical process is: the wolfberry pigment powder mixes with the matrimony vine seed ratio of pulverizing 40 mesh sieves at 3.5: 1, puts into supercritical CO ₂Extraction kettle → control pressure 25～27Mpa, 40 ℃～49 ℃ extractions of temperature → the first extraction-container control pressure 9Mpa, temperature is resolved → the second extraction-container control pressure 6～8Mpa, 25 ℃～35 ℃ parsing → β-Hu Luobusus of temperature (content 64%) for 36 ℃～40 ℃.

1.25t fresh matrimony vine berry can prepare the 1t Fructus Lycii original juice, isolates 200kg wolfberry pigment cream, vacuum lyophilization goes out wolfberry pigment powder 20kg, extracts the β-Hu Luobusu 985g of content 64% with 6kg matrimony vine seed.

4, Fructus Lycii original juice through 500,000 molecular weight membrane sepn after, again through 100,000 molecular weight membrane sepn, hold back down 〉=the Chinese wolfberry clear juice 400kg of 100,000 molecular weight about, can be used for producing Chinese wolfberry fruit drink 50t; Perhaps be used for producing matrimony vine light sparkling wine 40t; Perhaps be used for producing the low fermented wine 400kg of matrimony vine.

5, Fructus Lycii original juice through 100,000 molecular weight film extraction separation after, pass through contain lycium barbarum polysaccharide≤the Chinese wolfberry clear juice 300kg of 100,000 molecular weight, through after the vacuum lyophilization, obtain the lycium barbarum polysaccharide (crude product) about 30kg.Yield is 2.4% of a matrimony vine berry.Fructus Lycii original juice is loss about 10% in the extraction separation process.

New invention has following effect: lycium barbarum polysaccharide (crude product) the content height of 1, employing new technology and extracting, yield height.Lycium barbarum polysaccharide (crude product) content that " ethanol precipitation " extracts is: 55%～70%, and yield is: 2%～5%; And lycium barbarum polysaccharide (crude product) content that new technology is extracted is: 279%～352% (measures with ' sulfuric acid-phynol method ' that ' matrimony vine essence ' standard is examined, be a relative value), yield is: 2.4%, by 5kg matrimony vine berry oven dry 1kg wolfberry fruit conversion yield be: 12% (2.4% * 5=12%).2, employ new technology and extract lycium barbarum polysaccharide (crude product), can comprehensive development and utilization, for example: 1250kg matrimony vine berry, produce the 1000kg Fructus Lycii original juice; Isolate 25kg matrimony vine seed, separate freeze-drying and go out 20kg wolfberry pigment powder, can produce 2.85kg Boxthorn Seed Oil, 985g β-Hu Luobusu; Isolate the 400kg Chinese wolfberry clear juice, can produce Chinese wolfberry fruit drink 50000kg; Can also produce high-load lycium barbarum polysaccharide (crude product) 30kg.

And after 1250kg matrimony vine berry is dried into the 250kg wolfberry fruit, adopt lycium barbarum polysaccharide (crude product) 5～12.5kg that traditional technology ' ethanol precipitation ' also can only the extraction separation low levels.3, adopt new fresh fructus lycii to extract lycium barbarum polysaccharide, concerning the thatch farming of plantation matrimony vine, directly purchase fresh fructus lycii really than purchase wolfberry fruit cost efficient, and capital construction investment is low, dries about 380,000 yuan of matching construction scales of investment for one.If do not build drying plant, directly the airing worm climbs fly and stings extremely unhygienicly, and is subjected to weather phenomena to influence restriction, and is very big to the quality and the quality influence of wolfberry fruit.When the wolfberry fruit falling market, the direct marketing medlar fresh fruit can be protected the interests of thatch farming by it.4, adopt novel method technical process extraction separation lycium barbarum polysaccharide, LBP content height in the lycium barbarum polysaccharide (crude product) is suitable for declaring preparation three, four class new Chinese medicines most.All good for the state and the people, adapted to the development trend of China's entry into the WTO.

Claims (7)

1, lycium barbarum polysaccharide (crude product) extraction separation new technology is a raw material with the matrimony vine, extracts lycium barbarum polysaccharide (crude product).It is characterized in that: adopt fresh matrimony vine berry, after making beating is squeezed the juice, separate and remove the matrimony vine seed, the gained Fructus Lycii original juice is as the raw material of extraction separation lycium barbarum polysaccharide (crude product).Adopt membrane separation technique, separate wolfberry pigment and insoluble solid particulate with 500,000 molecular weight new membrane earlier; Also can manually hang filter and separate wolfberry pigment, use general 500,000 molecular weight films again, separate macromolecule pigment and insoluble solid earlier with the thick filter cloth of general 300 purposes; Then, use 100,000 molecular weight films again, dam and dissolved the Chinese wolfberry clear juice part of molecular weight lycium barbarum polysaccharide between 9900d～88000d, carry out vacuum lyophilization again and get lycium barbarum polysaccharide (crude product).

2, lycium barbarum polysaccharide according to claim 1 (crude product) extraction separation new technology, one of its feature is that the technological process of production of Fructus Lycii original juice is: pluck the drip washing → oxidation of purifying waste water of fresh matrimony vine berry → tap water flushing → cleaning machine and remove the pesticide residue → spray Cleaning for High Capacity of purifying waste water → squeeze the juice, remove seed, skin → defibrination → sterilization → sterile packed, refrigeration → Fructus Lycii original juice.

3, lycium barbarum polysaccharide according to claim 1 (crude product) extraction separation new technology, two of its feature are isolated matrimony vine seed earlier, adopt supercritical CO ₂Abstraction technique extracts Boxthorn Seed Oil.The technological process of production is: matrimony vine seed pulverizing → adding supercritical CO ₂Extraction kettle → control pressure, temperature extraction → control pressure, temperature parsing → Boxthorn Seed Oil.

4, lycium barbarum polysaccharide according to claim 1 (crude product) extraction separation new technology, three of its feature is from isolated macromolecule wolfberry pigment of Fructus Lycii original juice and insoluble solid, become the wolfberry pigment powder through vacuum lyophilization, as carrier, adopt supercritical CO with Boxthorn Seed Oil ₂Abstraction technique, the extraction separation β-Hu Luobusu.The technological process of production is: wolfberry pigment powder and crushed medlars seed add supercritical CO in proportion ₂Extraction kettle → control pressure, temperature extraction → control pressure, temperature parsing → β-Hu Luobusu.

5, lycium barbarum polysaccharide according to claim 1 (crude product) extraction separation new technology, four of its feature is to isolate from Fructus Lycii original juice the wolfberry pigment and the employed film of insoluble solid of 500,000 molecular weight, is a kind of Special Metal film that can separate thick material; Small-scale production also can be separated with the hanging of the thick filter cloth nature of common 300 purposes.

6, lycium barbarum polysaccharide according to claim 1 (crude product) extraction separation new technology, five of its feature is to isolate the used film of Chinese wolfberry clear juice below 100,000 molecular weight that contain lycium barbarum polysaccharide from Fructus Lycii original juice, be a kind of can hold back composition and by composition can both fine recovery special plate polymer membrane, the aperture must remain on the film of 100,000 molecular weight sizes, thickness unanimity.

7, lycium barbarum polysaccharide according to claim 1 (crude product) extraction separation new technology, six of its feature be from Fructus Lycii original juice isolate contain LBP≤Chinese wolfberry clear juice of 100,000 molecular weight, the cold-trap of vacuum freeze must temperature can reach subzero 60 ℃ medical equipment could fine freeze-drying go out lycium barbarum polysaccharide (crude product).