CN101911951A - Medical disinfectant composition for rearing instrument in silkworm rearing room and application thereof - Google Patents
Medical disinfectant composition for rearing instrument in silkworm rearing room and application thereof Download PDFInfo
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- CN101911951A CN101911951A CN 201010241825 CN201010241825A CN101911951A CN 101911951 A CN101911951 A CN 101911951A CN 201010241825 CN201010241825 CN 201010241825 CN 201010241825 A CN201010241825 A CN 201010241825A CN 101911951 A CN101911951 A CN 101911951A
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Abstract
The invention relates to a medical disinfectant composition for a rearing instrument in a silkworm rearing room and application thereof, belonging to the technical field of control of silkworm diseases. The medical disinfectant composition for the rearing instrument in the silkworm rearing room comprises the following components in part by weight: 1 part of dibromohydantoin and 2-10 parts of anhydrous sodium carbonate. The components are mixed uniformly to obtain the medical disinfectant composition. The invention further relates to a water solution containing the medical disinfectant composition for the rearing instrument in the silkworm rearing room, which comprises the following components in part by weight: 1 part of dibromohydantoin, 2-10 parts of anhydrous sodium carbonate and 500-2000 parts of water. The medical disinfectant composition for the rearing instrument in the silkworm rearing room has favorable killing effect on pathogens of silkworm viruses, funguses, bacteria and the like, can effectively prevent the virus diseases, the fungus diseases and the bacterial diseases of silkworms, and is easily decomposed into non-poisonous materials without accumulated toxicity and environmental pollution.
Description
Technical field
The present invention relates to a kind of silkworm room disinfection of tools for silkworm rearing pharmaceutical composition and application thereof, belong to silkworm disease control technical field.
Background technology
China is quality raw materials cocoon production base, the world, and export volume accounts for more than 80% of the world.The direct economic loss that sericulture causes because of flacherie in producing accounts for 10～15% of total income, and virus disease and mycosis are main diseases.Disinfectant commonly used is many based on chlorinated product, formaldehyde preparation, and toxicity is big, excitant is strong, has influenced the healthy of sericulture personnel to a certain extent, and environment is also caused certain harm.Disclose a kind of silkworm medication and preparation method thereof as Chinese patent CN1353933 (application number is 01128910.4), a kind ofly specifically be used to prevent and treat the silkworm medication of silkworm common disease and the preparation method of this silkworm medicine.It is to contain the medicament that carbendazim and thiophanate methyl raw material are made, and the weight portion proportioning of its raw material is carbendazim powder 6-8, thiophanate methyl powder 2-4; By proportioning described raw material is made powder-type; By proportioning described raw material is dissolved in respectively and makes the liquor type in spirit of vinegar and the ethanol.This silkworm medication has unique killing action to tame muscardine, pus illness, bright edema etc.Along with people to the attention of health and the enhancing of environmental consciousness, low toxicity, silkworm becomes inevitable requirement with disinfecting drug efficiently.
C5H6Br2N2O2, chemical name: 1,3-two bromo-5,5-dimethyl hydantion, molecular formula: C
5H
6Br
2N
2O
2Be called for short: DBDMH, mainly as sterilization, algicide, can effectively kill various bacteriums, fungi, virus, algae, hepatitis viruse, Escherichia coli, staphylococcus aureus, gonorrhoea, cholera, salmonella typhimurium etc., can be widely used in the aquaculture, effectively prevent and treat various bacillary, the fungoid disease of fish, soft-shelled turtle, crab, shrimp, the frog, shellfish etc.; The disinfection that also is used for industry water, running water, sanitary sewage, swimming pool.This product is a kind of special bromating agent, compare with bromating agents such as N-bromoacetamide, N-bromo-succinimides have active bromine content height, excellent storage stability, use economic dispatch advantage, be widely used in the bromination on pi-allyl in chemical industry and the pharmaceuticals industry and benzyl compounds and the active aromatic ring, also as disinfection or sanitizer etc.
The oxidized form environmental protection broad-spectrum sterilization medicament of new generation that C5H6Br2N2O2 (DBDMH) is generally acknowledge in the world at present most advanced in all water treatment agents, sterilizing power is the strongest.(C5H6Br2N2O2 discharges effective bromine of bactericidal action in disinfecting process owing to its environmental protection, the residue dimethyl hydantoin decomposes ammonification and carbonic acid gas, environmentally safe), nontoxic (chmice acute per os LD50＞5000mg/kg, the nontoxic level in true border) low flavor, safety broad-spectrum, usage amount is little, sterilizing power is extremely strong, and stability is high, not influenced by the pH value and water condition limits, and slowly discharge the halogen source, the validity cycle is long, replaces conventional chlorinated product just rapidly, becomes optimal disinfection product.
Though the C5H6Br2N2O2 disinfecting power is extremely strong, but when being used for the sterilization of silkworm breed silkworm room rearing instrument, but can't kill bombyx mori nuclear polyhydrosis virus (silkworm NPB), silkworm cytoplasmic polyhedrosis virus (silkworm CPB), have a strong impact on the application of C5H6Br2N2O2 as silkworm room disinfection of tools for silkworm rearing medicine.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, a kind of low toxicity silkworm room disinfection of tools for silkworm rearing pharmaceutical composition and preparation method thereof efficiently is provided.
The present invention is achieved through the following technical solutions:
A kind of silkworm room disinfection of tools for silkworm rearing pharmaceutical composition, component is as follows, is weight portion:
1 part of C5H6Br2N2O2,2～10 parts of natrium carbonicum calcinatums;
With the said components mixing promptly.
Preferably, described natrium carbonicum calcinatum is 5～10 parts.
The aqueous solution of a kind of above-mentioned silkworm room disinfection of tools for silkworm rearing pharmaceutical composition, component is as follows, is weight portion:
1 part of C5H6Br2N2O2,2～10 parts of natrium carbonicum calcinatums, 500～2000 parts in water;
With the said components mixing promptly.
Preferably, described natrium carbonicum calcinatum is 5～10 parts.
The application of above-mentioned silkworm room disinfection of tools for silkworm rearing pharmaceutical composition in silkworm room disinfection of tools for silkworm rearing and silkworm breed diseases prevention.
Above-mentioned application, step is as follows:
(1) add doubly by 500～2000 of C5H6Br2N2O2 weight in the disinfection of tools for silkworm rearing pharmaceutical composition of silkworm room and add water, mix, silkworm room disinfection of tools for silkworm rearing pharmaceutical composition solution;
(2) the silkworm room disinfection of tools for silkworm rearing pharmaceutical composition solution that step (1) is made is by 200～300ml/m
2The amount of spraying spray on the rearing instrument of silkworm room and get final product.
The application of the aqueous solution of above-mentioned silkworm room disinfection of tools for silkworm rearing pharmaceutical composition in silkworm room disinfection of tools for silkworm rearing and silkworm breed diseases prevention.
Above-mentioned application is directly pressed 200～300ml/m with the aqueous solution of silkworm room disinfection of tools for silkworm rearing pharmaceutical composition
2The amount of spraying spray on the rearing instrument of silkworm room and get final product.
The present invention has following advantage:
1, the invention solves the drawback that C5H6Br2N2O2 is applied to can't kill in the silkworm breed sterilizing prevent disease bombyx mori nuclear polyhydrosis virus (silkworm NPB), silkworm cytoplasmic polyhedrosis virus (silkworm CPB), make this product after using once, can kill various silkworm pathogenic germs, do not need to mend and execute other medicament.
2, C5H6Br2N2O2 and natrium carbonicum calcinatum are the low toxicity chemical substance, and it is efficient to have a low toxicity, easily degraded, and the characteristics of environmentally safe, and it is little to the toxic and side effect of silkworm body to use the back, helps the medicine person's of spraying self protection.
3, this product application process is simple, and cost is low, helps applying on a large scale.
Embodiment
Below in conjunction with embodiment the present invention is further elaborated, but institute of the present invention protection domain is not limited thereto.
C5H6Br2N2O2 described in the embodiment is available from Longkou Keda Chemical Co., Ltd., and natrium carbonicum calcinatum is available from Tianjin Standard Science Co., Ltd.
Embodiment 1
A kind of silkworm room disinfection of tools for silkworm rearing pharmaceutical composition is characterized in that component is as follows:
C5H6Br2N2O2: 10g, natrium carbonicum calcinatum: 100g; With C5H6Br2N2O2 and natrium carbonicum calcinatum mixing promptly.
Embodiment 2
The silkworm room disinfection of tools for silkworm rearing pharmaceutical composition that embodiment 1 is made adds water to gross weight 10kg, and dissolving mixes, and promptly gets the aqueous solution of silkworm room disinfection of tools for silkworm rearing pharmaceutical composition.
The 10g C5H6Br2N2O2 is added water to gross weight 10kg, and dissolving mixes, and promptly gets to contrast the aqueous solution.
Below the Disinfection Effect of the aqueous solution of silkworm room disinfection of tools for silkworm rearing pharmaceutical composition is done following experiment:
A, white muscardine fungi test
The medication group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) aqueous solution 200 microlitres of 200 microlitres and silkworm room disinfection of tools for silkworm rearing pharmaceutical composition mix, be coated with after 10 minutes to play the silkworm body surface 2 ages to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 200 microlitres mix with the contrast aqueous solution 200 microlitres, be coated with after 10 minutes and play the silkworm body surface 2 ages to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
B, Aspergillus test
The medication group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) aqueous solution 200 microlitres of 200 microlitres and silkworm room disinfection of tools for silkworm rearing pharmaceutical composition mix, and are coated with the newly-hatched silkworm body surface after 20 minutes to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 200 microlitres mix with the contrast aqueous solution 200 microlitres, are coated with the newly-hatched silkworm body surface after 20 minutes to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
C, bombyx mori nuclear polyhydrosis virus (silkworm NPB) test
The medication group:
With silkworm NPB cause of disease liquid (cause of disease liquid concentration: 5.0 * 10
7Individual/ml) aqueous solution 200 microlitres of 200 microlitres and silkworm room disinfection of tools for silkworm rearing pharmaceutical composition mix, and are applied to the mulberry leaf positive and negative after 10 minutes to the moistening newly-hatched silkworm of feeding;
Control group:
With silkworm NPB cause of disease liquid (cause of disease liquid concentration: 5.0 * 10
7Individual/ml) 200 microlitres mix with the contrast aqueous solution 200 microlitres, are applied to the mulberry leaf positive and negative after 10 minutes to the moistening newly-hatched silkworm of feeding;
Above-mentioned experiment all raise two the length of time " Invest, Then Investigate " incidence of disease.
D, silkworm cytoplasmic polyhedrosis virus (silkworm CPB) test
The medication group:
With silkworm CPB cause of disease liquid (cause of disease liquid concentration: 5.0 * 10
7Individual/ml) aqueous solution 200 microlitres of 200 microlitres and silkworm room disinfection of tools for silkworm rearing pharmaceutical composition mix, and are applied to the mulberry leaf positive and negative after 10 minutes to the moistening newly-hatched silkworm of feeding;
Control group:
With silkworm CPB bacterium liquid (cell concentration: 5.0 * 10
7Individual/ml) 200 microlitres mix with the contrast aqueous solution 200 microlitres, are applied to the mulberry leaf positive and negative after 10 minutes to the moistening newly-hatched silkworm of feeding;
This experiment raising " Invest, Then Investigate " incidence of disease in three length of time.
E, bacillus thuringiensis (bt) experiment
The medication group:
With bacillus thuringiensis bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) aqueous solution 200 microlitres of 200 microlitres and silkworm room disinfection of tools for silkworm rearing pharmaceutical composition mix, and dip in transfer needle after 20 minutes and draw for 5 ages and play the silkworm percutaneous puncture-inoculation, normally feed;
Control group:
With bacillus thuringiensis bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 200 microlitres mix with the contrast aqueous solution 200 microlitres, after 20 minutes with transfer needle dip in draw 5 age the silkworm percutaneous puncture-inoculation, normally feed;
One day " Invest, Then Investigate " incidence of disease is raised in this experiment.
Experimental result is as shown in table 1:
Table 1
Embodiment 3
A kind of silkworm room disinfection of tools for silkworm rearing pharmaceutical composition is characterized in that component is as follows:
C5H6Br2N2O2: 10g, natrium carbonicum calcinatum: 50g; With C5H6Br2N2O2 and natrium carbonicum calcinatum mixing promptly.
Embodiment 4
The silkworm room disinfection of tools for silkworm rearing pharmaceutical composition that embodiment 3 is made adds water to gross weight 10kg, and dissolving mixes, and promptly gets the aqueous solution of silkworm room disinfection of tools for silkworm rearing pharmaceutical composition.
The 10g C5H6Br2N2O2 is added water to gross weight 10kg, and dissolving mixes, and promptly gets to contrast the aqueous solution.
Below the Disinfection Effect of the aqueous solution of silkworm room disinfection of tools for silkworm rearing pharmaceutical composition is done following experiment:
A, white muscardine fungi test
The medication group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) aqueous solution 200 microlitres of 200 microlitres and silkworm room disinfection of tools for silkworm rearing pharmaceutical composition mix, be coated with after 30 minutes to play the silkworm body surface 2 ages to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 200 microlitres mix with the contrast aqueous solution 200 microlitres, be coated with after 30 minutes and play the silkworm body surface 2 ages to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
B, Aspergillus test
The medication group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) aqueous solution 200 microlitres of 200 microlitres and silkworm room disinfection of tools for silkworm rearing pharmaceutical composition mix, and are coated with the newly-hatched silkworm body surface after 30 minutes to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 200 microlitres mix with the contrast aqueous solution 200 microlitres, are coated with the newly-hatched silkworm body surface after 30 minutes to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
C, bombyx mori nuclear polyhydrosis virus (silkworm NPB) test
The medication group:
With silkworm NPB cause of disease liquid (cause of disease liquid concentration: 5.0 * 10
7Individual/ml) aqueous solution 200 microlitres of 200 microlitres and silkworm room disinfection of tools for silkworm rearing pharmaceutical composition mix, and are applied to the mulberry leaf positive and negative after 30 minutes to the moistening newly-hatched silkworm of feeding;
Control group:
With silkworm NPB cause of disease liquid (cause of disease liquid concentration: 5.0 * 10
7Individual/ml) 200 microlitres mix with the contrast aqueous solution 200 microlitres, are applied to the mulberry leaf positive and negative after 30 minutes to the moistening newly-hatched silkworm of feeding;
Above-mentioned experiment all raise two the length of time " Invest, Then Investigate " incidence of disease.
D, silkworm cytoplasmic polyhedrosis virus (silkworm CPB) test
The medication group:
With silkworm CPB cause of disease liquid (cause of disease liquid concentration: 5.0 * 10
7Individual/ml) aqueous solution 200 microlitres of 200 microlitres and silkworm room disinfection of tools for silkworm rearing pharmaceutical composition mix, and are applied to the mulberry leaf positive and negative after 30 minutes to the moistening newly-hatched silkworm of feeding;
Control group:
With silkworm CPB bacterium liquid (cell concentration: 5.0 * 10
7Individual/ml) 200 microlitres mix with the contrast aqueous solution 200 microlitres, are applied to the mulberry leaf positive and negative after 30 minutes to the moistening newly-hatched silkworm of feeding;
This experiment raising " Invest, Then Investigate " incidence of disease in three length of time.
E, bacillus thuringiensis (bt) experiment
The medication group:
With bacillus thuringiensis bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) aqueous solution 200 microlitres of 200 microlitres and silkworm room disinfection of tools for silkworm rearing pharmaceutical composition mix, and dip in transfer needle after 30 minutes and draw for 5 ages and play the silkworm percutaneous puncture-inoculation, normally feed;
Control group:
With bacillus thuringiensis bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 200 microlitres mix with the contrast aqueous solution 200 microlitres, after 30 minutes with transfer needle dip in draw 5 age the silkworm percutaneous puncture-inoculation, normally feed;
One day " Invest, Then Investigate " incidence of disease is raised in this experiment.
Experimental result is as shown in table 2:
Table 2
Claims (8)
1. a silkworm room disinfection of tools for silkworm rearing pharmaceutical composition is characterized in that component is as follows, is weight portion:
1 part of C5H6Br2N2O2,2～10 parts of natrium carbonicum calcinatums;
With the said components mixing promptly.
2. pharmaceutical composition as claimed in claim 1 is characterized in that, described natrium carbonicum calcinatum is 5～10 parts.
3. the aqueous solution of a silkworm room as claimed in claim 1 disinfection of tools for silkworm rearing pharmaceutical composition is characterized in that component is as follows, is weight portion:
1 part of C5H6Br2N2O2,2～10 parts of natrium carbonicum calcinatums, 500～2000 parts in water;
With the said components mixing promptly.
4. the aqueous solution as claimed in claim 3 is characterized in that, described natrium carbonicum calcinatum is 5～10 parts.
5. the application of the described silkworm room of claim 1 disinfection of tools for silkworm rearing pharmaceutical composition in silkworm room disinfection of tools for silkworm rearing and silkworm breed diseases prevention.
6. application as claimed in claim 5 is characterized in that step is as follows:
(1) add doubly by 500～2000 of C5H6Br2N2O2 weight in the disinfection of tools for silkworm rearing pharmaceutical composition of silkworm room and add water, mix, silkworm room disinfection of tools for silkworm rearing pharmaceutical composition solution;
(2) the silkworm room disinfection of tools for silkworm rearing pharmaceutical composition solution that step (1) is made is by 200～300ml/m
2The amount of spraying spray on the rearing instrument of silkworm room and get final product.
7. the application of the aqueous solution of the described silkworm room of claim 3 disinfection of tools for silkworm rearing pharmaceutical composition in silkworm room disinfection of tools for silkworm rearing and silkworm breed diseases prevention.
8. application as claimed in claim 7 is characterized in that, directly the aqueous solution of silkworm room disinfection of tools for silkworm rearing pharmaceutical composition is pressed 200～300ml/m
2The amount of spraying spray on the rearing instrument of silkworm room and get final product.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103636677A (en) * | 2013-12-23 | 2014-03-19 | 莫海仪 | Disinfectant for killing silkworm viruses |
| CN104604868A (en) * | 2015-01-04 | 2015-05-13 | 山东省蚕业研究所 | Disinfectant for silkworm rearing rooms, silkworm rearing instruments and silkworm rearing beds and application of disinfectant |
| CN105123701A (en) * | 2015-07-24 | 2015-12-09 | 华南农业大学 | Mulberry leaf disinfecting medicine composition, preparation method and application thereof |
| CN106804619A (en) * | 2016-11-30 | 2017-06-09 | 河池市技术开发中心 | A kind of disinfection of silkworm rearing room agent containing C5H6Br2N2O2 |
| CN106889107A (en) * | 2017-01-24 | 2017-06-27 | 辽宁省蚕业科学研究所 | A kind of toothed oak garden disinfectant and application method |
| CN109497087A (en) * | 2019-01-09 | 2019-03-22 | 安徽省农业科学院蚕桑研究所 | A kind of silkworm room disinfection of tools for silkworm rearing liquid without formaldehyde |
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| CN1410363A (en) * | 2002-11-08 | 2003-04-16 | 无锡中顺生物技术有限公司 | Manufacturing method of granular dibromohydantoin for killing harmful bacteria in aquaculture water |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103636677A (en) * | 2013-12-23 | 2014-03-19 | 莫海仪 | Disinfectant for killing silkworm viruses |
| CN104604868A (en) * | 2015-01-04 | 2015-05-13 | 山东省蚕业研究所 | Disinfectant for silkworm rearing rooms, silkworm rearing instruments and silkworm rearing beds and application of disinfectant |
| CN105123701A (en) * | 2015-07-24 | 2015-12-09 | 华南农业大学 | Mulberry leaf disinfecting medicine composition, preparation method and application thereof |
| CN106804619A (en) * | 2016-11-30 | 2017-06-09 | 河池市技术开发中心 | A kind of disinfection of silkworm rearing room agent containing C5H6Br2N2O2 |
| CN106889107A (en) * | 2017-01-24 | 2017-06-27 | 辽宁省蚕业科学研究所 | A kind of toothed oak garden disinfectant and application method |
| CN109497087A (en) * | 2019-01-09 | 2019-03-22 | 安徽省农业科学院蚕桑研究所 | A kind of silkworm room disinfection of tools for silkworm rearing liquid without formaldehyde |
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