CN101773136B - Pesticide preparation of rotenone mixed with antibiotic bactericide - Google Patents

Abstract

The invention discloses a pesticide preparation mixed with rotenone and antibiotic bactericide, which is prepared by mixing 0.5-50% by weight of rotenone, 0.5-55% by weight of antibiotic bactericide and the balance of auxiliary agents. The antibiotic fungicide is blasticidin. The present invention utilizes the synergistic effect between the active ingredient of rotenone and the antibiotic bactericide to make up for the deficiency of a single dose of pesticide, achieve the purpose of improving the effect of the pesticide, and expand the scope of use of the pesticide. The mixed pesticide preparation of the present invention is easy to use and improves the prevention It is safe for humans, animals and the ecological environment, and is a kind of mixed fungicide suitable for popularization and use.

Description

Pesticide preparation mixed with rotenone and antibiotic fungicide

This application is a divisional application of the Chinese patent application with application number 200710032746.0. The original application date was December 21, 2007, and the application number was 200710032746.0.

technical field

The invention belongs to the technical field of pesticides, in particular to a pesticide preparation mixed with rotenone and antibiotic bactericide.

Background technique

At present, most of the pesticides used in agriculture to prevent and control active diseases are chemical fungicides. These chemical fungicides also pollute the environment, kill a large number of beneficial natural enemies, cause human and animal poisoning, and easily make diseases and insects resistant. Therefore, it is an urgent need for agricultural production to develop high-efficiency, low-toxicity, and environmentally safe fungicides.

Rotenone is a compound from the root of the plant Rotenone or the leaves of edamame. Its mechanism of action is to inhibit the respiratory chain system of organisms. It has certain bactericidal activity and degrades quickly in the environment without harming natural enemies and other non-target organisms. An excellent biopesticide, its disadvantage is that it is unstable and has less processed formulations.

Antibiotic fungicides are highly effective and safe, but diseases are prone to develop resistance to them.

In a large number of long-term research experiments, the applicant expects to further obtain a rotenone mixed fungicide that can expand the control spectrum, reduce the dosage, and have various dosage forms after reasonable mixing of rotenone and antibiotic fungicides.

Contents of the invention

The purpose of the present invention is to fill up the gap in the prior art and provide a safe and efficient pesticide preparation mixed with rotenone and antibiotic fungicides. Through the reasonable mixing of rotenone and antibiotic fungicides, the antibacterial spectrum is expanded, the efficacy of the medicine is improved, the dosage of the medicine is reduced, and the pollution to the environment is reduced.

The technical solution of the invention is to provide a pesticide preparation mixed with rotenone and antibiotic bactericide, which is formed by mixing 0.05-70% by weight of rotenone, 0.05-70% by weight of antibiotic bactericide and the balance of auxiliary agents.

The rotenone can be one or more of the original drug, the plant extract containing rotenone or the rotenone preparation, and when using more than one, the total usage amount cannot be greater than the maximum value of the portion.

Described antibiotic bactericide is aureonuclemycin (aureonuclemycin), aureofungin, blasticidin (blasticidin-S), cycloheximide (cycloheximide), griseofulvin (griseofulvin), kasugamycin (kasugamycin), sodium One or more of natamycin, polyoxins, polyoxorim, streptomycin or validamycin. When using more than one, the total amount used should not be greater than the maximum serving size. Its bactericidal active ingredient can be original medicine and/or preparation.

As a preferred version, the mixed pesticide preparation of the present invention consists of 0.5-50% by weight of rotenone, 0.5-50% by weight of aureofungin or 1-50% by weight of aureofungin or blasticide 0.5-55% by weight of cycloheximide or 0.5-55% by weight of cycloheximide or 0.5-55% by weight of griseofulvin or 0.5-55% by weight of kasugamycin or 0.5-55% by weight of natamycin 0.5-60% by weight or polyoxorimin 0.5-55% by weight or polyoxorim 0.5-55% by weight or streptomycin sulfate 0.5-55% by weight or validamycin 0.5-55% by weight, It is mixed with the rest of the additives.

Preferably, the mixed pesticide formulation of rotenone and antibiotic bactericide is mixed with the following components in weight percentage:

Rotenone 0.5%;

Blasticidin 9.5%;

Humectant Sodium Lauryl Sulfate 25%;

Dispersant alkyl sulfonate 22%;

Carrier kaolin balance.

The beneficial effects of the present invention are:

1. After rotenone is mixed with antibiotic fungicides, it can overcome the shortcomings of chemical fungicides that are prone to resistance, broaden the scope of use of the medicine, extend the duration of efficacy, and improve the efficacy of the medicine.

2. After the rotenone is mixed with the aforementioned antibiotic and bactericide, the dosage is reduced, and the production cost is reduced under the premise of greatly improving the drug effect.

3. The main components of the preparation of this type of fungicide are high-efficiency and low-toxic biological source pesticides, which can greatly reduce the poisonous effect on various beneficial natural enemies, have little pollution to the ecological environment, and are conducive to protecting the safety of humans and animals.

Detailed ways

The technical content of the invention will be further described in detail below in conjunction with specific embodiments.

Embodiment 1: the preparation of 2.5% rotenone+aureofungin emulsifiable concentrate, the weight ratio of each component is:

Rotenone is 0.05%;

aureofungin is 2.45%;

Surfactant: dodecylphenol polyoxyethylene ether is 15%;

Co-solvent: methanol is 15%;

Anti-photolysis agent: castor oil is 1%;

Stabilizer: epoxidized soybean oil is 17%;

Penetrant: Laurocaprazine is 12%;

Organic solvent: make up to 100% xylene.

Put each component into the preparation kettle and mix thoroughly to obtain the product.

Embodiment 2: the preparation of 2.5% rotenone+aureofungin emulsifiable concentrate, the weight ratio of each component is:

Rotenone is 2.45%;

aureofungin at 0.05%;

Surfactant: dodecylphenol polyoxyethylene ether is 15%;

Co-solvent: methanol is 15%;

Anti-photolysis agent: castor oil is 1%;

Stabilizer: epoxidized soybean oil is 17%;

Penetrant: Laurocaprazine is 12%;

Organic solvent: make up to 100% xylene.

Put each component into the preparation kettle and mix thoroughly to obtain the product.

Embodiment 3: the preparation of 3% rotenone+aureofungin emulsifiable concentrate, the weight ratio of each component is:

Rotenone is 0.3%;

2.7% for aureofungin;

Surfactant: dodecylphenol polyoxyethylene ether is 15%;

Co-solvent: methanol is 15%;

Anti-photolysis agent: castor oil is 1%;

Stabilizer: epoxidized soybean oil is 17%;

Penetrant: Laurocaprazine is 12%;

Organic solvent: make up to 100% xylene.

Put each component into the preparation kettle and mix thoroughly to obtain the product.

Embodiment 4: the preparation of 75% rotenone + aureomycin wettable powder, the weight ratio of each component is:

Rotenone is 70%;

Aureomycin is 5%;

Wetting agent: sodium lauryl sulfate is 5%;

Dispersant: 10% of alkyl sulfonate;

Carrier: Kaolin supplemented to 100%.

The components are dried, cooled, mixed and ground to obtain the product.

Embodiment 5: the preparation of 75% rotenone+natamycin wettable powder, the weight ratio of each component is:

Rotenone is 5%;

Natamycin is 70%;

Wetting agent: sodium lauryl sulfate is 5%;

Dispersant: 10% of alkyl sulfonate;

Carrier: Kaolin supplemented to 100%.

The components are dried, cooled, mixed and ground to obtain the product.

Embodiment 6: the preparation of 10% rotenone + blasticidin wettable powder, the weight ratio of each component is:

Rotenone is 0.5%;

Blasticidin is 9.5%;

Wetting agent: sodium lauryl sulfate is 25%;

Dispersant: 22% of alkyl sulfonate;

Carrier: Kaolin supplemented to 100%.

The components are dried, cooled, mixed and ground to obtain the product.

Embodiment 7: the preparation of 30% rotenone+cycloheximide oil suspension concentrate, the weight ratio of each component is:

Rotenone is 10%;

Cycloheximide is 20%;

Dispersant: sodium lignosulfonate is 10%;

Viscosifier: bentonite is 3%;

Stabilizer: xanthan gum is 2%;

Defoamer: organic silicone is 5%;

Solvent: castor oil up to 100%.

Embodiment 8: the preparation of 20% rotenone+cycloheximide microemulsion, the weight ratio of each component is:

Rotenone is 5%;

Cycloheximide is 15%;

Emulsifier: calcium salt of alkylbenzenesulfonate is 20%;

Stabilizer: cresyl glycidyl ether is 13%;

Solvent: butanone is 20%;

Antifreeze: 3% sorbitol;

Make up to 100% with deionized water.

Stir the mixture in a stirrer, heat and invert the phase to obtain the product.

Embodiment 9: the preparation of 50% rotenone+polyoxin water dispersible granules, the weight ratio of each component is:

Rotenone is 8%;

Polyoxin was 42%;

Wetting agent: sodium lignosulfonate is 8%;

Binder: 9% polyvinyl acetate;

Disintegrant: urea is 15%;

Carrier: Activated clay supplemented to 100%.

The mixture is ultrafinely pulverized, then granulated, dried and sieved to obtain the product.

Embodiment 10: the preparation of 50% rotenone+polyoxorim water-dispersible granules, the weight ratio of each component is:

Rotenone is 20%;

30% polyoxorim;

Wetting agent: sodium lignosulfonate is 8%;

Binder: 9% polyvinyl acetate;

Disintegrant: urea is 15%;

Carrier: Activated clay supplemented to 100%.

The mixture is ultrafinely pulverized, then granulated, dried and sieved to obtain the product.

Embodiment 11: the preparation of 50% rotenone+streptomycin sulfate water-dispersible granules, the weight ratio of each component is:

Rotenone is 30%;

Streptomycin sulfate is 20%;

Wetting agent: sodium lignosulfonate is 8%;

Binder: 9% polyvinyl acetate;

Disintegrant: urea is 15%;

Carrier: Activated clay supplemented to 100%.

The mixture is ultrafinely pulverized, then granulated, dried and sieved to obtain the product.

Example 12: the preparation of 40% rotenone+effectivemycin water-dispersible granules, the weight ratio of each component is:

Rotenone is 20%;

Effective mycin is 20%;

Wetting agent: sodium lignosulfonate is 8%;

Binder: 9% polyvinyl acetate;

Disintegrant: urea is 15%;

Carrier: Activated clay supplemented to 100%.

The mixture is ultrafinely pulverized, then granulated, dried and sieved to obtain the product.

Embodiment 13: the preparation of 50% rotenone+griseofulvin water-dispersible granules, the weight ratio of each component is:

Rotenone is 8%;

Griseofulvin is 42%;

Wetting agent: sodium lignosulfonate is 8%;

Binder: 9% polyvinyl acetate;

Disintegrant: urea is 15%;

Carrier: Activated clay supplemented to 100%.

The mixture is ultrafinely pulverized, then granulated, dried and sieved to obtain the product.

Embodiment 14: the preparation of 25% rotenone+kasugamycin microcapsules, the weight ratio of each component is:

Rotenone is 7%;

Kasugamycin was 18%;

Dispersant: 10% polyvinyl alcohol;

Capsule wall material: gelatin supplemented to 100%.

The components are mixed and stirred at high speed for 2 hours, the polymer is separated by salting out, heated to form microcapsules, and then freeze-dried to obtain the product.

The mixed pesticide preparations prepared in the above examples are compared with the single-dose pesticides according to the routine toxicity test in the laboratory, and the results are shown in Table 1.

Table 1 Indoor toxicity test results of each mixed agent against cucumber downy mildew

Pharmacy Dilution factor Inhibition rate 2.5% Rotenone EC 1000 42.6% 5% aureofungin suspension concentrate 1500 56.4% 5% aureomycin suspension concentrate 1500 58.1% 5% natamycin suspension 1500 49.3% 5% blasticidin suspension concentrate 1500 48.2% 5% cycloheximide suspension concentrate 1500 38.5% 5% polyoxin suspension 1500 57.4% 5% polyoxorim suspension concentrate 1500 55.8%

5% streptomycin sulfate suspension concentrate 1500 62.5% 5% validamycin suspension 1500 61.4% 5% griseofulvin suspension concentrate 1500 52.8% 5% Kasugamycin Suspension Concentrate 1500 61.7% 0.05% rotenone + 2.45% aureofungin EC 2000 77.4% 2.45% rotenone + 0.05% aureofungin EC 2000 81.3% 0.3% rotenone + 2.7% aureofungin EC 2000 72.9% 70% rotenone + 5% aureomycin wettable powder 10000 100% 5% Rotenone + 70% Natamycin WP 10000 100% 0.5% rotenone + 9.5% blasticidin wettable powder 5000 83.4% 10% rotenone + 20% cycloheximide oil suspension 8000 90.3% 5% rotenone + 15% cycloheximide microemulsion 6000 93.4% 8% rotenone + 42% polyoxin water dispersible granules 10000 100% 20% rotenone + 30% polyoxofim water dispersible granules 10000 100% 30% rotenone + 20% streptomycin sulfate water dispersible granules 10000 100% 20% rotenone + 20% validamycin water dispersible granules 8000 100% 8% rotenone + 42% griseofulvin water dispersible granules 10000 100% 7% rotenone + 18% kasugamycin microcapsules 6000 98.4%

Example 14 Effect Experiment of Mixed Pesticide Preparations of Rotenone and Antibiotic Bactericides

Experimental method: use rotenone, aureomycin, aureofungin, blasticidin, cycloheximide, griseofulvin, kasugamycin, natamycin, polyoxin, polyoxorim, streptomycin sulfate, effective mold The single agent and the mixed agent of the element were made into liquid medicines of different concentrations to treat downy mildew of cucumber, and clear water was used as a control. Calculate the _EC50 and co-toxicity coefficient of the agent. For example, if the co-toxicity coefficient is close to 100, it indicates an additive effect, if it is greater than 100, it indicates a synergistic effect, and if it is less than 100, it indicates an antagonistic effect.

The experimental results are shown in Table 2 below.

Table 2 Indoor toxicity of rotenone and antibiotic fungicide mixture to cucumber downy mildew

Pharmacy ratio _EC50 (μg/ml) 95% Confidence Limits Co-toxicity coefficient Rotenone 182.16 176.2~208.5 0 Aureomycin 110.23 102.3-135.7 0 Rotenone: Aureomycin (14:1) 79.35 65.12-98.54 132.5 aureofungin 67.4 58.28~93.2 0 Rotenone: aureofungin (1:9) 46.3 35.9~69.2 142.8 blasticidin 85.5 73.1~122.3 0 Rotenone: blasticidin (1:19) 56.2 42.9~83.6 122.7 Cycloheximide 42.8 34.8~55.5 0 Rotenone: Cycloheximide (1:2) 31.5 27.3~40.4 148.1 Griseofulvin 57.3 44.9~74.1 0 Rotenone: griseofulvin (1:5) 33.5 26.4~42.5 123.4 Kasugamycin 35.2 25.13-43.97 0 Rotenone: Kasugamycin (2:5) 21.65 18.43-25.91 157.93 Natamycin 72.53 64.21-90.37 0 Rotenone: Natamycin (1:14) 48.81 35.68-57.35 137.2 Polyoxin 71.94 65.39-92.85 0 Rotenone: Polyoxin (1:5) 47.91 32.62-71.20 148.4

polyoxorim 39.52 24.61-52.67 0 Rotenone: polyoxorim (2:3) 21.57 16.73-38.91 158.2 streptomycin sulfate 57.24 48.19-72.81 0 Rotenone: streptomycin sulfate (3:2) 31.38 20.43-45.81 138.5 Validamycin 58.29 41.38-72.93 0 Rotenone:effectivemycin (1:1) 36.89 23.76-59.14 126.8

It can be seen from Table 2 that after mixing rotenone and several antibiotics and fungicides, the co-toxicity coefficients against cucumber downy mildew are all greater than 100, indicating that they have a synergistic effect.

Claims (2)

1. A pesticide preparation mixed with rotenone and antibiotic bactericide is characterized in that it is formed by mixing 0.5 to 50% by weight of rotenone, 0.5 to 55% by weight of antibiotic bactericide and a surplus of auxiliary agents. The antibiotic bactericide is blasticidin; the weight ratio of rotenone and blasticidin is 1:19. 2. according to the described rotenone of claim 1 and antibiotic bactericide mixed pesticide preparation, it is characterized in that being mixed by each component of following percentage by weight: Rotenone 0.5%; Blasticidin 9.5%; Humectant sodium lauryl sulfate 25%; Dispersant alkyl sulfonate 22%; Carrier kaolin balance.