CN101743971B - Glutaraldehyde Peracetic Acid Compound Disinfectant - Google Patents

Abstract

The invention relates to a disinfector, in particular to a compound disinfector of peroxyacetic acid and glutaric dialdehyde. The compound disinfector is mixed liquid, comprising peroxyacetic acid, hydrogen peroxide, glutaric dialdehyde, purified water, stabilizing agent and synergist. The compound disinfector is prepared by the following steps: taking 75-90ml of peroxyacetic acid, 10-23ml of hydrogen peroxide, 0.2-3ml of glutaric dialdehyde, 0.5-5ml of polyoxyethylene fatty alcohol ether and 0.1-3g of sodium pyrophosphate respectively, mixing fully and stirring evenly. The invention has the characteristics of high speed, high efficiency, long acting, low cost, small toxicity, no corrosiveness, no pollution and strong anti-jamming ability. The disinfector can quickly and thoroughly kill microorganisms in animal colony houses and the environment and has a special killing function to Avian influenza viruses.

Description

Glutaraldehyde Peracetic Acid Compound Disinfectant

technical field

The invention relates to a disinfectant, in particular to a compound disinfectant of glutaraldehyde peroxyacetic acid.

Background technique

Disinfection is an effective means to kill various pathogenic microorganisms in the environment. Peracetic acid, chlorine dioxide, povidone iodine, chlorine-containing disinfectants, aldehyde disinfectants, etc. have good killing effects on pathogenic microorganisms under experimental conditions, but these single-component disinfectants are limited by their own properties in actual use. , environmental temperature, the presence of organic matter, pH value, etc., can not achieve the ideal disinfection effect, there are inevitable shortcomings. Oxidative disinfectants such as peracetic acid and hydrogen peroxide decompose to produce various free radicals such as OH groups and active oxygen derivatives, which destroy the permeability barrier of microorganisms, proteins, amino acids, enzymes and DNA, etc., leading to the death of microorganisms. Disinfectants have the advantages of wide bactericidal spectrum, low effective use concentration, and fast action speed, but they are greatly affected by environmental factors and organic matter, corrosive to copper, stainless steel, galvanized iron, etc., and the effective time of disinfection is short. Glutaraldehyde can cross-link with cell wall lipoproteins and form side chains with lipid-linked residues in cell wall phosphoric acid, thereby sealing the cell wall and hindering the absorption of nutrients by microorganisms and the discharge of waste. It can be used in livestock and poultry and pens It has the advantages of low concentration and super strong bactericidal effect at room temperature, can be quickly dissolved in water, the product performance is stable, the drug effect can be maintained for two weeks, the toxic and side effects are small, and it will not damage the utensils and disinfection facilities. It is not affected by the external environment and can be widely used in various occasions. However, the disinfectant is highly corrosive and highly irritating to humans and animals, and it takes 30 minutes for glutaraldehyde to reach a high level of disinfection.

Based on the above reasons, technicians are constantly trying to use the characteristics of different types of disinfectants and their synergistic ratios to develop a rapid, efficient, long-acting, low-cost, stable property and will not cause damage and pollution to the environment. Compound disinfectant. For example, the technology of disinfection made with hydrogen peroxide and acetic acid as the main ingredient has been documented, such as: application (patent) number: 200510042902.2 discloses a compound peroxyacid disinfectant and its processing technology is prepared by following raw materials and technology Ingredients: ① Raw materials: hydrogen peroxide: 55%-70% acetic acid: 12%-23% sulfuric acid: 2%-4% octanoic acid: 1%-2%, surfactant 2%-11%, organic solvent 4%-17 % make a compound peroxyacid disinfectant containing peracetic acid, peroxyoctanoic acid and hydrogen peroxide. Patent No. 200810053995.2 also discloses a disinfection process, which talks about utilizing 25-30 parts of acetic acid and 12-20 parts of hydrogen peroxide to be used as a disinfectant (to generate corresponding peracetic acid). In existing literature reports, glutaraldehyde is generally used as raw materials to prepare composite disinfectants with quaternary ammonium salts.

Contents of the invention

The present invention provides a glutaraldehyde peroxyacetic acid compound disinfectant according to the synergistic effect of different types of disinfectants in order to overcome the defects in the use of a single disinfectant.

The present invention is realized by the following technical solutions, a peracetic acid glutaraldehyde compound disinfectant is composed of the following raw materials: 75-90 parts by volume of peracetic acid, 10-23 parts by volume of hydrogen peroxide, and 0.2-23 parts by volume of glutaraldehyde. 3 parts by volume, 0.5-5 parts by volume of polyoxyethylene fatty alcohol ether, and 0.1-3 parts by mass of sodium pyrophosphate. 1 part by mass corresponds to 1 part by volume, for example, 1 g corresponds to 1 ml, and 1 kg corresponds to 1 L.

The specific implementation process is: dilute the peracetic acid stock solution to 2% with purified water, take 75-90 parts, add hydrogen peroxide, glutaraldehyde, polyoxyethylene fatty alcohol ether in turn, and finally add 0.1-3 g of sodium pyrophosphate , and mix well. The raw materials used in the present invention can be purchased from the market.

Peracetic acid (CH ₃ COOOH), also known as peracetic acid, is a strong oxidizing agent, a colorless transparent liquid, easily soluble in water, alcohol and sulfuric acid. When peracetic acid encounters organic matter or enzymes, it releases nascent oxygen. This nascent oxygen achieves bactericidal effect through two ways. One is to denature and solidify the bacterial protein; the other is to damage the active gene of the enzyme protein through redox reaction. , Inhibit the activity of the enzyme, or because the chemical structure is similar to the metabolite, it can be combined with the enzyme competitively or non-competitively to inhibit the activity of the enzyme. Peracetic acid has a good bactericidal effect.

Hydrogen peroxide, commonly known as hydrogen peroxide, has a molecular formula of H ₂ O ₂ , and the concentration of commercially available hydrogen peroxide is 30%. Hydrogen peroxide is a strong oxidant, and its anti-virus principle is: various free radicals generated after H ₂ O ₂ decomposition, such as OH groups, active oxygen derivatives, etc., destroy the permeability barrier of microorganisms, proteins, amino acids, enzymes, etc. and DNA, etc. eventually lead to the death of microorganisms.

Glutaraldehyde is a 5-carbon bisacetal compound with a molecular formula of C ₅ H ₈ O ₂ , which has a typical chemical reaction of aldehydes. The bactericidal principle mainly relies on the aldehyde group, which mainly acts on the sulfhydryl group, hydroxyl group, carboxyl group and amino group of the bacterial protein, which can be alkylated, causing protein coagulation and bacterial death.

Peracetic acid and hydrogen peroxide are used as strong oxidants, and glutaraldehyde is used as a protein coagulant. After compounding, aldehyde peroxide is formed to enhance the bactericidal effect. At the same time, the stability of peracetic acid can be enhanced in the presence of a large amount of hydrogen peroxide. Disinfectants can not only kill viruses and sterilize efficiently but also have a synergistic effect with each other, and oxidizing disinfectants have a strong effect and can kill viruses and bacteria quickly, but they are easily affected by organic matter. The disinfection effect of glutaraldehyde is less affected by organic matter, and the effective time of disinfection is long. Compounding it can improve the disinfection effect.

The disinfectant provided by the invention can be widely used in the disinfection of various breeding industries of animal husbandry and veterinary medicine, will not cause damage and pollution to the environment, and has far-reaching significance for environmental protection and solving the drug resistance of microorganisms. Compared with existing single-component disinfectants, the present invention has the following characteristics and advantages:

1. Fast speed of action and strong lethality

(1) The disinfection effect on avian influenza virus (AIV) is obviously better than that of a single component, and the disinfectant can be diluted 10000 times for 10 minutes or diluted 5000 times for 5 minutes to inactivate more than 99.99% of AIV. The disinfectant contains the same dose of peracetic acid and hydrogen peroxide for 10 minutes and glutaraldehyde for 5 minutes, which can only inactivate 99.97% of AIV.

(2) For bacteria, it can completely kill bacterial propagules within 5 to 30 minutes, and can effectively kill Bacillus cereus spores within 30 minutes, achieving a sterilization effect.

2. Long duration of action

The effective duration of disinfection with 10,000 dilutions is 48 hours, and doubling the concentration of disinfectant can extend the effective duration of disinfection to 72 hours. The inactivation rate of pathogenic microorganisms is significantly higher than that of peracetic acid, glutaraldehyde and hydrogen peroxide, prolonging the effective disinfection duration.

3. Low toxicity and non-corrosive

The toxicity test of this product through mouth, skin and eyes: It shows that the compound disinfectant is practically non-toxic; it is slightly irritating to the skin and safe and harmless to the human body. Corrosion experiments show that it is basically non-corrosive to stainless steel, carbon steel, and copper immersion.

4. Stable in nature and strong in anti-interference ability, the inactivation rate of compound disinfectant on microorganisms is basically not affected by temperature, pH value and organic matter in the environment.

5. Low cost

Under the condition that the compound disinfectant achieves the same disinfection effect on the tested microorganisms, the cost is 4% to 21% of the cost of the raw material medicine of the disinfectant.

6. The formula has a simple process and is very favorable for industrialized production.

The results of several years of experimental research on the disinfectant show that the compound disinfectant has a significant inactivation effect on pathogenic microorganisms such as AIV virus and Escherichia coli.

Scheme: 80-85 parts by volume of peracetic acid, 15-20 parts by volume of hydrogen peroxide, 1-2 parts by volume of glutaraldehyde, 3-4 parts by volume of polyoxyethylene fatty alcohol ether, 1-2 parts by volume of sodium pyrophosphate . For specific experimental objects, the results are shown in the table below:

Table 1 The results of the inactivation of AIV by disinfectants

Table 2 Compound disinfectant qualitative sterilization test result

Detailed ways

Embodiment 1: get 75ml after peracetic acid stock solution is diluted to 2% with purified water, add hydrogen peroxide 20ml and glutaraldehyde 3ml successively, polyoxyethylene fatty alcohol ether 2ml, make its final volume be 100ml, add coke Sodium phosphate 0.6g, fully stir and mix well.

Embodiment 2: get 82ml after peracetic acid stock solution is diluted to 2% with purified water, add hydrogen peroxide 15ml and glutaraldehyde 1.5ml successively, polyoxyethylene fatty alcohol ether 1.5ml, make its final volume be 100ml, then Add 1g of sodium pyrophosphate, stir well and mix well.

Embodiment 3: get 85ml after peracetic acid stock solution is diluted to 2% with purified water, add hydrogen peroxide 13ml and glutaraldehyde 1ml successively, polyoxyethylene fatty alcohol ether 1ml, make its final volume be 100ml, add coke Sodium phosphate 1.6g, fully stir and mix well.

Example 4, after diluting the peracetic acid stock solution to 2% with purified water, take 90ml, add hydrogen peroxide 10ml and glutaraldehyde 3ml, polyoxyethylene fatty alcohol ether 0.5ml, then add sodium pyrophosphate 3g, fully stir Just mix well.

Example 5, after diluting the peracetic acid stock solution to 2% with purified water, get 75ml, add hydrogen peroxide 23ml and glutaraldehyde 0.2ml, polyoxyethylene fatty alcohol ether 5ml, then add sodium pyrophosphate 0.1g, fully Stir to combine.

Claims (2)

1. A peracetic acid glutaraldehyde compound disinfectant is characterized in that it is composed of the following raw materials in proportion: 75 to 90 parts by volume of peracetic acid, 10 to 23 parts by volume of hydrogen peroxide, and 0.2 to 3 parts by volume of glutaraldehyde , 0.5-5 parts by volume of polyoxyethylene fatty alcohol ether and 0.1-3 parts by mass of sodium pyrophosphate, wherein 1 part by mass corresponds to 1 part by volume, and 1 g corresponds to 1 ml. 2. peracetic acid glutaraldehyde compound disinfectant according to claim 1 is characterized in that it is made up of following raw material according to proportion: peracetic acid 80～85 parts by volume, hydrogen peroxide 15～20 parts by volume, glutaraldehyde 1-2 parts by volume, 3-4 parts by volume of polyoxyethylene fatty alcohol ether and 1-2 parts by mass of sodium pyrophosphate.