CN101049098A - Culture medium for preserving species of mites - Google Patents
Culture medium for preserving species of mites Download PDFInfo
- Publication number
- CN101049098A CN101049098A CNA2007100892329A CN200710089232A CN101049098A CN 101049098 A CN101049098 A CN 101049098A CN A2007100892329 A CNA2007100892329 A CN A2007100892329A CN 200710089232 A CN200710089232 A CN 200710089232A CN 101049098 A CN101049098 A CN 101049098A
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- CN
- China
- Prior art keywords
- mite
- medium
- culture medium
- mites
- agar
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000894007 species Species 0.000 title claims abstract description 11
- 241000238876 Acari Species 0.000 title abstract description 12
- 239000001963 growth medium Substances 0.000 title abstract description 11
- 229920001817 Agar Polymers 0.000 claims abstract description 11
- 239000008272 agar Substances 0.000 claims abstract description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000000178 monomer Substances 0.000 claims abstract description 6
- 229920000742 Cotton Polymers 0.000 claims abstract description 4
- 229920002472 Starch Polymers 0.000 claims abstract description 3
- 239000001913 cellulose Substances 0.000 claims abstract description 3
- 229920002678 cellulose Polymers 0.000 claims abstract description 3
- 239000000203 mixture Substances 0.000 claims abstract description 3
- 229930014626 natural product Natural products 0.000 claims abstract description 3
- 239000001814 pectin Substances 0.000 claims abstract description 3
- 229920001277 pectin Polymers 0.000 claims abstract description 3
- 235000010987 pectin Nutrition 0.000 claims abstract description 3
- 239000008107 starch Substances 0.000 claims abstract description 3
- 235000019698 starch Nutrition 0.000 claims abstract description 3
- 235000000346 sugar Nutrition 0.000 claims abstract description 3
- 239000000843 powder Substances 0.000 claims abstract 4
- 238000004321 preservation Methods 0.000 claims abstract 3
- 102000004169 proteins and genes Human genes 0.000 claims abstract 2
- 108090000623 proteins and genes Proteins 0.000 claims abstract 2
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 239000004563 wettable powder Substances 0.000 claims description 5
- 239000006013 carbendazim Substances 0.000 claims description 4
- 235000010980 cellulose Nutrition 0.000 claims description 2
- 239000005416 organic matter Substances 0.000 claims description 2
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 1
- 230000000855 fungicidal effect Effects 0.000 claims 1
- 239000000417 fungicide Substances 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 claims 1
- 150000008163 sugars Chemical class 0.000 claims 1
- 239000002609 medium Substances 0.000 abstract description 10
- 235000013399 edible fruits Nutrition 0.000 abstract description 7
- 235000013305 food Nutrition 0.000 abstract description 4
- 241000132121 Acaridae Species 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 241000260534 Carpoglyphidae Species 0.000 abstract description 2
- 230000002068 genetic effect Effects 0.000 abstract description 2
- 244000052769 pathogen Species 0.000 abstract description 2
- 230000000844 anti-bacterial effect Effects 0.000 abstract 1
- 239000003899 bactericide agent Substances 0.000 abstract 1
- 235000013312 flour Nutrition 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 208000028804 PERCHING syndrome Diseases 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- -1 4 grams Substances 0.000 description 1
- 241000305049 Ascidae Species 0.000 description 1
- 241000040710 Chela Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000232956 Laelapidae Species 0.000 description 1
- 241000406602 Macrochelidae Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000269800 Percidae Species 0.000 description 1
- 241001481699 Phytoseiidae Species 0.000 description 1
- 241000611866 Tyrophagus putrescentiae Species 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明涉及一种螨类保种的培养基，是在带有棉花塞的透明容器内加入水、广谱性杀菌剂经热溶后结冻的琼脂平面或斜面培养基，培养基中还可加入糖类、淀粉、纤维素、果胶、蛋白、酵母粉等有机物单体或含多种单体的混合物或自然产物。该培养基其优点：(1)降低受其他螨类或病菌污染的机会，(2)有利于建立具有遗传多样性的捕食螨种群，(3)把它装入透明的容器中便于观察。同时以琼脂等制作成斜面培养基，作为粉螨Acaridae或果螨Carpoglyphidae的栖息依附材料和食料，培育粉螨或果螨作为捕食螨的饲料，饲养捕食螨。The present invention relates to a culture medium for acarid species preservation, which is an agar plane or slope culture medium which is frozen by adding water and a broad-spectrum bactericide into a transparent container with a cotton plug, and the culture medium can also be Add sugar, starch, cellulose, pectin, protein, yeast powder and other organic monomers or mixtures containing multiple monomers or natural products. The medium has the advantages of: (1) reducing the chance of being polluted by other mites or pathogens, (2) helping to establish a population of predatory mites with genetic diversity, and (3) putting it into a transparent container for easy observation. At the same time, agar is used to make a slant medium, which is used as the inhabiting material and food for the acaridae or the fruit mite Carpoglyphidae, to cultivate the acaridae or the fruit mite as the feed for the predatory mite, and to raise the predatory mite.
Description
Technical field
The present invention relates in the biological control technical field natural enemy and breed cultivation, relate generally to a kind of culture medium for preserving species of mites.
Background technology
So far still nobody develops or delivers the medium that is used for mite class guarantor kind.
At present the guarantor of mite class plants the main food processing byproducts of using, and for example: wheat bran, rice bran etc. mixes with wood chip, straw etc., perches as non-predacious mites and breeds environment and food, as Tyroglyphidae or eat sweet mite section mite class etc.Breeding or during the child care predacious mite, is with above-mentioned environment and the Tyroglyphidae of breeding or to eat sweet mite section mite class be environment and food, promptly inserts the predation mite in the environment again above-mentioned breeding, and mixes to raise and protects kind.This is feed with agricultural product or agro-product processing by product, and directly forage mites being protected the method for planting together with the predation mite has many deficiencies, even causes the destruction of population.Its major defect is: (1) is difficult for observing, and is difficult to grasp the current intelligence of the growth of predation mite, growth and population, and (2) breeding of method is subjected to the pollution of non-purpose mite class or germ easily, and (3) are easy to generate population and degenerate.
Summary of the invention
The purpose of this invention is to provide a kind of medium that the mite class is protected kind that is suitable for, promptly a kind ofly not only make things convenient for observation but also can suitably control kind and the medium of population development quantity.
The objective of the invention is to realize as follows: a kind of culture medium for preserving species of mites is characterized in that being contained in and has agar plate or a slant medium that is added with broad-spectrum germicide in the cotton plug transparent vessel.
Above-mentioned a kind of culture medium for preserving species of mites also can add the edible organic matter monomer of suitable mite classes such as sugar, starch, cellulose, pectin, albumen, dusty yeast or contain the mixture or the natural product of multiple monomer in its medium.
Above-mentioned culture medium for preserving species of mites, its preferred culture medium consist of agar, sucrose, dusty yeast, 50% carbendazol wettable powder, water.
Above-mentioned a kind of culture medium for preserving species of mites, its agar: sucrose: dusty yeast: carbendazim wettable powder: the water ratio is 5: 10: 15: 1: 250.
Adopt its advantage of such scheme medium: (1) reduces the chance that is subjected to other mite classes or pathogen contamination, (2) microcommunity of this medium culture of some is mixed, help setting up the predation mite population with genetic diversity, (3) are packed it into and are convenient in the transparent container observe.Simultaneously be made into slant medium, depend on material and foodstuff, cultivate flour mite or fruit mite feed, raise the predation mite as the predation mite as flour mite Acaridae or perching of fruit mite Carpoglyphidae with agar etc.
The present invention can be used for flour mite or perching of mite of fruit depended on material and foodstuff; After inserting flour mite or fruit mite, can be used as and go through the Laelapidae of mite section, the huge chela mite Macrochelidae of section and the capsule mite Ascidae of section predacious mites, and guarantor's kind of some Phytoseiidae Phytoseiidae mite classes, expand numerous and biological study on a small scale.
Embodiment
Embodiment 1:
About 1000 milliliters of agar 20 gram, sucrose 40 grams, dusty yeast 60 grams, 50% carbendazol wettable powder, 4 grams, water.Sucrose, dusty yeast are added in the entry, and the slow fire heated and stirred is dissolved the back and is added agar, continues heated and stirred to dissolving fully, adds carbendazim, stirs and supply the water yield to 1000mL.Use the multilayer filtered through gauze, divide immediately to install in the test tube, the branch loading amount is 1/5 of a test tube height, behind cotton plug, the wrapping mark, puts into the inclined-plane, cooling beyond the Great Wall.Connect kind of a mite during use, be placed on 20-22 ℃ of unglazed interior or indoor shady and cool dark place of incubator of shining and get final product.Before inserting predacious mites, should insert flour mite or fruit mite earlier as feed.
Claims (4)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100892329A CN101049098A (en) | 2007-03-15 | 2007-03-15 | Culture medium for preserving species of mites |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100892329A CN101049098A (en) | 2007-03-15 | 2007-03-15 | Culture medium for preserving species of mites |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101049098A true CN101049098A (en) | 2007-10-10 |
Family
ID=38780872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100892329A Pending CN101049098A (en) | 2007-03-15 | 2007-03-15 | Culture medium for preserving species of mites |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101049098A (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101790972A (en) * | 2010-03-15 | 2010-08-04 | 于丽辰 | Method for feeding Tarsonemus confusus |
| CN102461481A (en) * | 2010-11-16 | 2012-05-23 | 复旦大学 | Long-term maintenance culture method for tropical acarus tropicalis |
| CN102511452A (en) * | 2011-12-31 | 2012-06-27 | 中国农业科学院植物保护研究所 | Novel method for artificial mass rearing of Amblyseius orientalis |
| CN103181368A (en) * | 2012-09-18 | 2013-07-03 | 上海雪榕生物科技股份有限公司 | Method for detecting and controlling acarid during fungus mushroom cultivation |
| CN103299958A (en) * | 2013-05-20 | 2013-09-18 | 中国农业科学院植物保护研究所 | Novel method for feeding prey flour mites and predatory mites |
| CN103734089A (en) * | 2013-12-26 | 2014-04-23 | 中山大学 | Artificial breeding method of predatory mites of phytoseiidae |
| CN104222661A (en) * | 2014-09-26 | 2014-12-24 | 张可池 | Nutrition-supplementing additive for predatory mite |
| CN106818650A (en) * | 2017-02-20 | 2017-06-13 | 湖北勤劳农夫生态农业股份有限公司 | Predatory Mites are cultivated using greenhouse and discharge method to control insect pest on the spot |
| CN107114620A (en) * | 2017-06-12 | 2017-09-01 | 湖北勤劳农夫生态农业股份有限公司 | A kind of Predatory Mites cultivation base-material and its application method |
| CN107156546A (en) * | 2017-06-12 | 2017-09-15 | 湖北勤劳农夫生态农业股份有限公司 | A kind of Predatory Mites high-quality introduces a collection watches material |
| CN107494625A (en) * | 2017-09-26 | 2017-12-22 | 江苏菇本堂生物科技股份有限公司 | A kind of acarid for planting edible mushroom detects liquid |
| CN109699834A (en) * | 2017-10-25 | 2019-05-03 | 石河子大学 | A kind of man-made feeds of the new small peaceful mite of double tails |
| CN111543397A (en) * | 2020-06-23 | 2020-08-18 | 安徽国际旅行卫生保健中心(合肥海关口岸门诊部) | A kind of rapid proliferation and cultivation method of Tyrosum scavenger mites |
-
2007
- 2007-03-15 CN CNA2007100892329A patent/CN101049098A/en active Pending
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101790972A (en) * | 2010-03-15 | 2010-08-04 | 于丽辰 | Method for feeding Tarsonemus confusus |
| CN101790972B (en) * | 2010-03-15 | 2011-11-16 | 于丽辰 | Method for feeding Tarsonemus confusus |
| CN102461481A (en) * | 2010-11-16 | 2012-05-23 | 复旦大学 | Long-term maintenance culture method for tropical acarus tropicalis |
| CN102511452A (en) * | 2011-12-31 | 2012-06-27 | 中国农业科学院植物保护研究所 | Novel method for artificial mass rearing of Amblyseius orientalis |
| CN103181368B (en) * | 2012-09-18 | 2015-02-04 | 上海雪榕生物科技股份有限公司 | Method for detecting and controlling acarid during fungus mushroom cultivation |
| CN103181368A (en) * | 2012-09-18 | 2013-07-03 | 上海雪榕生物科技股份有限公司 | Method for detecting and controlling acarid during fungus mushroom cultivation |
| CN103299958A (en) * | 2013-05-20 | 2013-09-18 | 中国农业科学院植物保护研究所 | Novel method for feeding prey flour mites and predatory mites |
| CN103734089A (en) * | 2013-12-26 | 2014-04-23 | 中山大学 | Artificial breeding method of predatory mites of phytoseiidae |
| CN104222661A (en) * | 2014-09-26 | 2014-12-24 | 张可池 | Nutrition-supplementing additive for predatory mite |
| CN106818650A (en) * | 2017-02-20 | 2017-06-13 | 湖北勤劳农夫生态农业股份有限公司 | Predatory Mites are cultivated using greenhouse and discharge method to control insect pest on the spot |
| CN107114620A (en) * | 2017-06-12 | 2017-09-01 | 湖北勤劳农夫生态农业股份有限公司 | A kind of Predatory Mites cultivation base-material and its application method |
| CN107156546A (en) * | 2017-06-12 | 2017-09-15 | 湖北勤劳农夫生态农业股份有限公司 | A kind of Predatory Mites high-quality introduces a collection watches material |
| CN107114620B (en) * | 2017-06-12 | 2020-10-02 | 湖北勤劳农夫生态农业股份有限公司 | Predatory mite culture base material and using method thereof |
| CN107494625A (en) * | 2017-09-26 | 2017-12-22 | 江苏菇本堂生物科技股份有限公司 | A kind of acarid for planting edible mushroom detects liquid |
| CN109699834A (en) * | 2017-10-25 | 2019-05-03 | 石河子大学 | A kind of man-made feeds of the new small peaceful mite of double tails |
| CN111543397A (en) * | 2020-06-23 | 2020-08-18 | 安徽国际旅行卫生保健中心(合肥海关口岸门诊部) | A kind of rapid proliferation and cultivation method of Tyrosum scavenger mites |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20071010 |