CN100506276C - Production method of cottonseed polypeptide dry powder with antioxidant effect - Google Patents

Abstract

A process for preparing the antioxidizing cottonseed polypeptide powder used as the nutrient intensifier for improving immunity includes such steps as mixing cottonseed protein with distilled water, grinding, regulating pH value and temp, adding alkaline proteinase, enzymolyzing, adding flavouring proteinase, regulating pH value and temp, enzymolyzing, boiling for deactivating enzyme, cooling, filtering, vacuum concentrating, and spray drying.

Description

Production method of cottonseed polypeptide dry powder with antioxidant effect

technical field

The invention relates to a treatment method of cottonseed protein, in particular to a method for preparing a dry polypeptide powder with anti-oxidation function by using the cottonseed protein. The dry polypeptide powder belongs to functional and nutritious food.

Background technique

Cotton is one of the important crops in the world. Cottonseed is rich in more than 30% protein. Its amino acid composition is complete, the ratio is reasonable, and its nutritional value is close to that of bean protein. It is a very important plant protein resource. Biologically active peptides are polypeptides with amino acid sequences, which have special physiological activities on the life activities of biological organisms. Certain low-peptides can not only provide the nutrients needed for human growth and development, but also have the functions of preventing and treating diseases and regulating human physiological functions, such as lowering blood pressure, lowering cholesterol, promoting immunity and promoting the digestion and absorption of nutrients. Some are The unique physiological functions that the original food protein or its constituent amino acids do not have. The research and development of active peptides provides the possibility to use protein resources with low biological value to produce human health food base materials. At present, the shortage of food and protein supply in many areas of our country has become an unavoidable serious problem. Therefore, the development and utilization of secondary high-quality plant protein is particularly important. The preparation and significance of functional peptides using cottonseed as raw materials are very obvious. At present, the production of bioactive peptides mainly adopts enzymatic hydrolysis. Enzymatic hydrolysis can improve the physicochemical and functional properties of protein without reducing the nutritional value, and expand the application range in food processing. Moreover, the active peptides produced by enzymatic hydrolysis are extremely safe, cheap and easy to promote. There are many techniques for enzymatically hydrolyzing protein. Some use a single type of enzyme to hydrolyze, which takes a long time and consumes energy. As the hydrolysis time prolongs, the activity of the hydrolyzate gradually loses; some use multiple types of Enzymes are hydrolyzed at the same time, but by choosing different types of enzymes and their combinations, and using different hydrolysis conditions, the hydrolysates obtained are different, and the properties of the hydrolysates are also different; in addition, the protein polypeptides produced by enzymatic methods are often accompanied by bitter taste , which is also the main factor restricting the industrial production of polypeptides by enzymatic methods. For enzymatic production of cottonseed polypeptide, there are few reports in the literature at home and abroad, and the method of the present invention uses the compound enzymatic hydrolysis technology to hydrolyze cottonseed protein isolate to prepare cottonseed polypeptide with antioxidant activity.

Contents of the invention

The purpose of the present invention is to make up for the deficiencies in the prior art, and to provide a simple process, good activity of the hydrolyzate and easy control, and the obtained finished product has good anti-oxidation effect and stable performance, and is suitable for industrial production. The invention discloses a production method of cottonseed polypeptide dry powder.

The object of the present invention is to realize by following measures: the inventive method comprises the following steps successively:

1) Take one weight of cottonseed protein isolate, add 3 to 6 times the weight of distilled water, and grind it into a uniform cottonseed protein isolate slurry;

2) According to the weight of the prepared cottonseed protein isolate refining liquid, add 3 to 7 times the weight of distilled water, put it into the reaction tank, and adjust the pH value to 7.8 to 8.2 with an alkaline solution;

3) Keep the solution obtained in step 2) in a state of continuous stirring, add alkaline microbial protease according to 4-5% of the weight of cottonseed protein isolate obtained in step 1), and heat to 50-55°C for hydrolysis;

4) During the hydrolysis process, use an alkaline solution to continuously adjust and maintain the pH value of the hydrolyzed solution at 7.9 to 8.1, and continue to hydrolyze for 1 to 1.5 hours;

5) Adjust the pH value of the hydrolyzate that has been hydrolyzed for the first time in step 4) to 6.8-7.2 with an acidic solution, and keep the temperature at 48-52°C;

6) Add flavor protease to the hydrolyzate obtained in step 5) under continuous stirring according to 4-6% of the weight of cottonseed protein isolate taken in step 1), and maintain its pH value at 6.9-7.1 with an alkaline solution, and keep the temperature 48～52℃, continuous hydrolysis for 2～3 hours;

7) heating and boiling the hydrolyzed solution after the second hydrolysis in step 6) for 15 to 20 minutes to inactivate the enzyme;

8) Cool the hydrolyzed solution in step 7) to 70-80°C, filter it in a filter tank, concentrate the obtained filtrate in vacuum to 40-45 Brix (Baume degrees), and spray-dry the concentrated solution to obtain cottonseed with antioxidant effect Peptide dry powder.

The total protein content of the cottonseed protein isolate used above is generally 60-99%, and the content of gossypol≦0.06%; L of hydrochloric acid solution, using these two solutions to adjust the pH value of the hydrolyzate is simple, economical and effective; the label of the alkaline microbial protease used above is Alcalase, the enzyme activity is 2.4Au/g, and the label of the flavor protease used is Flavourzyme ^TM , the enzyme activity is 500LAPU/g, this enzyme has high specificity and stable performance. Compared with the prior art, the present invention has the following advantages:

The multi-stage directional hydrolysis of cottonseed protein isolate with complex enzymes is used, and the macromolecular proteins of different properties are hydrolyzed in stages with enzymes of different performances. The specificity of hydrolysis is enhanced, the utilization rate of enzymes is greatly improved, and the hydrolysis time is shortened. The consumption is reduced, and the activity of the hydrolyzate is effectively improved. The hydrolyzate has no obvious bitter taste, the production cost is relatively low, and the investment is relatively low. The product can not only be directly used as a high-grade protein nutritional supplement, but also as an acidic plant protein drink, sports It is an additive for food, baby food, military food, etc. It is a kind of natural health nutrition product with strong anti-oxidation that is better than similar products in the past, and has a wide range of uses.

Detailed ways

Embodiment 1, the production of the dry powder of cottonseed polypeptide with antioxidant effect

Take 20 kg of cottonseed protein isolate with a protein content of 60%, add 60 kg of distilled water, grind it into a fine and uniform cottonseed protein isolate slurry with a colloid mill, add 320 kg of distilled water, put it into the reaction tank, and use 1mol/L Sodium hydroxide solution to adjust its pH value to 7.9, add 1 kg of alkaline protease labeled Alcalase (in the state of continuous stirring, and heat to 54 ° C for hydrolysis, constantly use 1mol/L sodium hydroxide solution in the hydrolysis process Adjust and maintain the pH value of 8.0, keep the temperature at 54°C, and continue the hydrolysis for 1 hour. After the first hydrolysis, adjust its pH value to 7.0 with a molar concentration of 1mol/L hydrochloric acid solution, and adjust the temperature to 50°C. Add 1.2 kg of Flavourzyme ^TM flavor protease under constant stirring. During the hydrolysis process, use 1mol/L sodium hydroxide solution to adjust and keep the pH value at 7.0, keep the temperature at 50°C, and continue hydrolysis for 2.5 hours. After completion, heat and boil the hydrolyzate for 15 minutes to inactivate the enzyme, then turn on the condensed water outside the reaction tank, cool the hydrolyzate to 75°C, release the hydrolyzate to the filter tank for filtration, concentrate the obtained filtrate to 45Brix in vacuum, and spray the concentrate Dried into 100-mesh fine powder to obtain cottonseed polypeptide dry powder with anti-oxidation effect.

Embodiment 2, the production of the dry powder of cottonseed polypeptide with antioxidant effect

Take 18 kg of cottonseed protein isolate with a protein content of 70%, add 72 kg of distilled water, grind it into a fine and uniform cottonseed protein isolate slurry with a colloid mill, then add 280 kg of distilled water, put it into the reaction tank, and use 1mol/L Sodium hydroxide solution to adjust its pH value to 8.0, add 0.8 kg of alkaline protease labeled Alcalase under continuous stirring, and heat to 55°C for hydrolysis, and continuously use 1mol/L sodium hydroxide solution during the hydrolysis process Adjust and maintain the pH value at 7.9, maintain the temperature at 55° C., and continue the hydrolysis for 1.5 hours. After the first hydrolysis, adjust the pH value to 7.1 with a hydrochloric acid solution with a molar concentration of 1mol/L, adjust the temperature to 49°C, and add 0.8kg of Flavourzyme ^TM flavor protease under constant stirring. During the hydrolysis process Constantly use 1mol/L sodium hydroxide solution to adjust and keep the pH value at 7.1, keep the temperature at 49°C, and continue hydrolysis for 3 hours. After the hydrolysis is completed, heat and boil the hydrolyzate for 20 minutes to inactivate the enzyme, and then open the reaction tank. The condensed water is used to cool the hydrolyzate to 70°C, release the hydrolyzate to the filter tank for filtration, concentrate the obtained filtrate to 43Brix in vacuum, and spray-dry the concentrated solution into a 100-mesh fine powder to obtain the cottonseed polypeptide dry powder with antioxidant effect.

Claims (6)

1. A method for producing cottonseed polypeptide dry powder with antioxidant effect, characterized in that the method comprises the following steps in sequence: 1) Take one weight of cottonseed protein isolate, add 3 to 6 times the weight of distilled water, and grind it into a uniform cottonseed protein isolate slurry; 2) According to the weight of the prepared cottonseed protein isolate refining liquid, add 3 to 7 times the weight of distilled water, put it into the reaction tank, and adjust the pH value to 7.8 to 8.2 with an alkaline solution; 3) Keep the solution obtained in step 2) in a state of continuous stirring, add alkaline microbial protease according to 4-5% of the weight of cottonseed protein isolate obtained in step 1), and heat to 50-55°C for hydrolysis; 4) Constantly adjust with an alkaline solution during the hydrolysis process, and keep the pH value of the hydrolyzed solution at 7.9-8.1, and continue to hydrolyze for 1-1.5 hours; 5) Adjust the pH value of the hydrolyzate that has been hydrolyzed for the first time in step 4) to 6.8-7.2 with an acidic solution, and keep the temperature at 48-52°C; 6) Add flavor protease to the hydrolyzate obtained in step 5) under continuous stirring according to 4-6% of the weight of cottonseed protein isolate taken in step 1), and maintain its pH value at 6.9-7.1 with an alkaline solution, and keep the temperature 48～52℃, continuous hydrolysis for 2～3 hours; 7) heating and boiling the hydrolyzed solution after the second hydrolysis in step 6) for 15 to 20 minutes to inactivate the enzyme; 8) Cool the hydrolyzed solution in step 7) to 70-80°C, filter in a filter tank, concentrate the obtained filtrate in vacuum to 40-45 Brix, and spray dry the concentrated solution to obtain dry cottonseed polypeptide powder with antioxidant effect. 2. The method for producing cottonseed polypeptide dry powder with antioxidant effect according to claim 1, characterized in that: cottonseed protein isolate with a protein content of 60-99% and a gossypol content of ≦0.06% is used. 3. The method for producing cottonseed polypeptide dry powder with antioxidant effect according to claim 1, characterized in that: the alkaline solution used in step 2) or step 6) is a sodium hydroxide solution with a molar concentration of 1 mol/L . 4. The production method of cottonseed polypeptide dry powder with antioxidant effect according to claim 1, characterized in that: the label of the alkaline microbial protease used in step 3) is Alcalase, and the enzyme activity is 2.4Au/g. 5. The method for producing cottonseed polypeptide dry powder with antioxidant effect according to claim 1, characterized in that: the acidic solution used in step 5) is a hydrochloric acid solution with a molar concentration of 1 mol/L. 6. The production method of cottonseed polypeptide dry powder with antioxidant effect according to claim 1, characterized in that: the label of the flavor protease used in step 6) is Flavourzyme ^TM , and the enzyme activity is 500 LAPU/g.