CN1895661A - Preparation of cotton-seed polypeptide with anti-oxidant function - Google Patents
Preparation of cotton-seed polypeptide with anti-oxidant function Download PDFInfo
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- CN1895661A CN1895661A CNA2006100905875A CN200610090587A CN1895661A CN 1895661 A CN1895661 A CN 1895661A CN A2006100905875 A CNA2006100905875 A CN A2006100905875A CN 200610090587 A CN200610090587 A CN 200610090587A CN 1895661 A CN1895661 A CN 1895661A
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- protein isolate
- antioxidant effect
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 30
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 25
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 25
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title description 2
- 239000003963 antioxidant agent Substances 0.000 title 1
- 235000006708 antioxidants Nutrition 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 27
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 27
- 230000007062 hydrolysis Effects 0.000 claims abstract description 25
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 25
- 239000000843 powder Substances 0.000 claims abstract description 20
- 102000004190 Enzymes Human genes 0.000 claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 claims abstract description 19
- 235000012343 cottonseed oil Nutrition 0.000 claims abstract description 19
- 238000004519 manufacturing process Methods 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 15
- 108091005804 Peptidases Proteins 0.000 claims abstract description 11
- 239000004365 Protease Substances 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 10
- 239000012153 distilled water Substances 0.000 claims abstract description 9
- 230000008569 process Effects 0.000 claims abstract description 9
- 230000000694 effects Effects 0.000 claims abstract description 8
- 239000000796 flavoring agent Substances 0.000 claims abstract description 7
- 239000002002 slurry Substances 0.000 claims abstract description 6
- 235000019634 flavors Nutrition 0.000 claims abstract description 5
- 239000007921 spray Substances 0.000 claims abstract description 4
- 239000012141 concentrate Substances 0.000 claims abstract 2
- 239000000243 solution Substances 0.000 claims description 36
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- 239000012670 alkaline solution Substances 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 102000005158 Subtilisins Human genes 0.000 claims description 4
- 108010056079 Subtilisins Proteins 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 4
- 108010007119 flavourzyme Proteins 0.000 claims description 4
- QBKSWRVVCFFDOT-UHFFFAOYSA-N gossypol Chemical compound CC(C)C1=C(O)C(O)=C(C=O)C2=C(O)C(C=3C(O)=C4C(C=O)=C(O)C(O)=C(C4=CC=3C)C(C)C)=C(C)C=C21 QBKSWRVVCFFDOT-UHFFFAOYSA-N 0.000 claims description 4
- 230000000813 microbial effect Effects 0.000 claims description 4
- QHOPXUFELLHKAS-UHFFFAOYSA-N Thespesin Natural products CC(C)c1c(O)c(O)c2C(O)Oc3c(c(C)cc1c23)-c1c2OC(O)c3c(O)c(O)c(C(C)C)c(cc1C)c23 QHOPXUFELLHKAS-UHFFFAOYSA-N 0.000 claims description 2
- 229930000755 gossypol Natural products 0.000 claims description 2
- 229950005277 gossypol Drugs 0.000 claims description 2
- 239000003929 acidic solution Substances 0.000 claims 2
- 238000009835 boiling Methods 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000007670 refining Methods 0.000 claims 1
- 108091005658 Basic proteases Proteins 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 238000009776 industrial production Methods 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 19
- 210000000582 semen Anatomy 0.000 description 14
- 230000003064 anti-oxidating effect Effects 0.000 description 8
- 230000003301 hydrolyzing effect Effects 0.000 description 6
- 235000013305 food Nutrition 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 238000000227 grinding Methods 0.000 description 4
- 238000012423 maintenance Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 235000019658 bitter taste Nutrition 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000028016 temperature homeostasis Effects 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 235000008452 baby food Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000021245 dietary protein Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
本发明公开了具有抗氧化作用的棉籽多肽干粉生产方法，其步骤是以棉籽分离蛋白为原料，加入蒸馏水研磨成均匀的浆液，调节好对应的pH值和温度范围，加入碱性蛋白酶进行第一次酶解；然后加入风味蛋白酶，再次调整好对应的pH值和温度范围，进行第二次酶解；酶解完毕后加热煮沸使酶灭活，再经冷却、过滤和真空浓缩，最后喷雾干燥即得具有抗氧化作用的棉籽多肽干粉。该方法采用复合酶多级定向水解棉籽分离蛋白，水解产物易于控制，活性好，工艺简单，适于工业化生产，产品口感好，质量高，可直接食用，也可作为食品营养强化剂，具有抗氧化、增强免疫等功能。The invention discloses a production method of cottonseed polypeptide dry powder with antioxidant effect. The steps are to use cottonseed protein isolate as raw material, add distilled water to grind it into a uniform slurry, adjust the corresponding pH value and temperature range, and add alkaline protease for the first step. Second enzymolysis; then add flavor protease, adjust the corresponding pH value and temperature range again, and carry out the second enzymolysis; after enzymolysis, heat and boil to inactivate the enzyme, then cool, filter and concentrate in vacuum, and finally spray dry The cottonseed polypeptide dry powder with antioxidant effect is obtained. The method adopts multi-stage directional hydrolysis of cottonseed protein isolate with complex enzymes. The hydrolyzate is easy to control, has good activity, simple process, and is suitable for industrial production. Oxidation, enhance immunity and other functions.
Description
Technical field
The present invention relates to the processing method of cottonseed protein, specifically relate to utilize cottonseed protein to prepare and have the method for the polypeptide dry powder of antioxidation, polypeptide dry powder belongs to functional, nourishment food.
Background technology
Cotton Gossypii is one of important crops in the world, and Semen Gossypii is rich in albumen more than 30%, and its aminoacid is formed complete, and ratio is reasonable, and nutritive value is a kind of crucial plant protein resource near bean protein.Biologically active peptide is the polypeptide with amino acid sequence, and the vital movement of living organism is had special physiologically active.Some low peptide can not only provide the human body growth promoter required nutrient substance, and has the effect of preventing and curing diseases, regulating human physiological functions simultaneously, such as blood pressure lowering, cholesterol reducing, promote immunity and promote digesting and assimilating of nutrient substance that some is the physiological function of former food protein or the unexistent uniqueness of its composition aminoacid.The research and development of bioactive peptide provide possibility for utilizing the not high protein resource of biological value to produce human health food base material.At present, many regional foods of China and albumen are under-supply, become the serious problems that can't avoid, so the proteic development and use of secondary quality plant seem particularly important, are raw material with the Semen Gossypii, and the preparation and the meaning of carrying out Functional Polypeptides are fairly obvious.Enzyme hydrolysis method is mainly adopted in the production of biologically active peptide at present.Enzymatic hydrolysis can improve proteinic physics and chemistry and functional character under the prerequisite that does not reduce nutritive value, expansion is in the Application in Food Processing scope, and the bioactive peptide safety of enzymatic isolation method production high, cheap, be easy to promote.Technology with zymyhydrolyzed protein is existing multiple, the enzyme hydrolysis of the employing single variety that has, and the time is long, and during consumption energy consumption, along with the prolongation of hydrolysis time, the hydrolyzate activity is forfeiture gradually also; The hydrolysis simultaneously of the enzyme of a plurality of kinds of employing that have, but select the enzyme of different cultivars and assembly thereof, the different hydrolysising condition of employing, the hydrolyzate that is obtained is different, the characteristic that hydrolyzate has is also different; In addition, the protein polypeptide of Production by Enzymes is often with bitterness, and this also is the principal element of restriction enzyme process suitability for industrialized production polypeptide.For the Production by Enzymes cotton-seed polypeptide, domestic and international rarely seen bibliographical information, wherein the inventive method utilization complex enzyme hydrolysis technology hydrolysis Semen Gossypii protein isolate makes the cotton-seed polypeptide with antioxidant activity and yet there are no report.
Summary of the invention
The objective of the invention is to remedy the deficiencies in the prior art part, and provide a kind of technology simple, hydrolyzate is well active and be easy to control, the effective and stable performance of gained finished product antioxidation, and the enzyme process that is suitable for suitability for industrialized production is produced the production method of cotton-seed polypeptide dry powder.
The objective of the invention is to realize by following measure: the inventive method comprises the steps: successively
1) gets the Semen Gossypii protein isolate of a weight, add the distilled water of 3～6 times of weight, grind to form uniform Semen Gossypii protein isolate grinding slurry;
2) by the weight of prepared Semen Gossypii protein isolate grinding slurry, add the distilled water of 3～7 times of weight again, put in the retort, with alkaline solution adjust pH to 7.8～8.2;
3) with step 2) gained solution is in continuous stirring and gets under the state, and set by step 1) 4～5% of the Semen Gossypii protein isolate weight of getting, add alkaline microbial protease, and be heated to 50～55 ℃ and be hydrolyzed;
4) constantly regulating and keep the pH value of hydrolyzed solution with alkaline solution in hydrolytic process is 7.9～8.1, continues hydrolysis 1～1.5 hour;
5) hydrolyzed solution that first hydrolysis in the step 4) is finished is adjusted pH value to 6.8～7.2 with acid solution, and temperature keeps 48～52 ℃;
6) step 5) gained hydrolyzed solution is being continued under the state that stirs, set by step 1) 4～6% of the Semen Gossypii protein isolate weight of getting add flavor proteases, and to keep its pH value with alkaline solution be 6.9～7.1, and keeping temperature is 48～52 ℃, continues hydrolysis 2～3 hours;
7) the hydrolyzed solution heated and boiled that hydrolysis for the second time in the step 6) is finished is 15～20 minutes, makes enzyme-deactivating;
8) hydrolyzed solution in the step 7) is cooled to 70～80 ℃, to the filter pocket filtration, gained filtrate vacuum concentration to 40～45Brix (Baume degrees) will promptly get the cotton-seed polypeptide dry powder with antioxidation after the concentrated solution spray drying.
The total protein content of above-mentioned used Semen Gossypii protein isolate is generally 60-99%, gossypol content≤0.06%; Above-mentioned used alkaline solution is that molar concentration is the sodium hydroxide solution of 1mol/L, and used acid solution is that molar concentration is the hydrochloric acid solution of 1mol/L, adopts these two kinds of solution to regulate simple, the economy, effective of pH value of hydrolyzed solution; The label of above-mentioned used alkaline microbial protease is Alcalase, and enzyme activity is 2.4Au/g, and the label of used flavor protease is Flavourzyme
TM, enzyme activity is 500LAPU/g, this kind of enzyme specificity height, stable performance.
The present invention compared with prior art has following advantage:
Adopted the multistage directionally hydrolyzing Semen Gossypii of compound enzyme protein isolate, be hydrolyzed with the enzyme of different performance stage by stage at high molecular weight protein of different nature, the special finger degree of hydrolysis strengthens, greatly improved the utilization rate of enzyme, hydrolysis time shortens, energy consumption descends, effectively raise the activity of hydrolyzate, hydrolyzate does not have obvious bitterness, and production cost is relatively low, invests less, products made thereby not only can be directly as the high protein supplementary, also can be used as acidic vegetable protein drink, motion food, infant food, the additive of military supplies food etc. is a kind of stronger natural health nutriment of non-oxidizability of like product in the past that is better than, and has purposes widely.
The specific embodiment
Embodiment 1, has the production of the cotton-seed polypeptide dry powder of antioxidation
Get 20 kilograms of the Semen Gossypii protein isolates of protein content 60%, add 60 kilograms of distilled water, grind to form fine and smooth Semen Gossypii protein isolate grinding slurry uniformly with colloid mill, add 320 kilograms of distilled water again, drop in the reaction filling, with molar concentration is that to regulate its pH value be 7.9 for the sodium hydroxide solution of 1mol/L, under the state of continuous stirring, add label and be Alcalase alkaline protease 1kg (, and be heated to 54 ℃ and be hydrolyzed, constantly sodium hydroxide solution adjustment and the maintenance pH value with 1mol/L is 8.0 in hydrolytic process, keeping temperature is 54 ℃, continues hydrolysis 1 hour.After hydrolysis for the first time finishes, with molar concentration be the hydrochloric acid solution of 1mol/L to adjust its pH value be 7.0, thermoregulation is 50 ℃, the adding label is Flavourzyme under the state that constantly stirs
TMFlavor protease 1.2kg, constantly sodium hydroxide solution adjustment and the maintenance pH value with 1mol/L is 7.0 in hydrolytic process, keeping temperature is 50 ℃, continue hydrolysis 2.5 hours, after hydrolysis finishes, the heated and boiled hydrolyzed solution made enzyme-deactivating in 15 minutes, open reaction then and irritate outer condensed water, make hydrolyzed solution be cooled to 75 ℃, emitting hydrolyzed solution to filter pocket filters, gained filtrate vacuum concentration is spray dried to 100 purpose fine powders to 45Brix with concentrated solution, promptly gets the cotton-seed polypeptide dry powder with antioxidation.
Embodiment 2, has the production of the cotton-seed polypeptide dry powder of antioxidation
Get 18 kilograms of the Semen Gossypii protein isolates of protein content 70%, add 72 kilograms of distilled water, grind to form fine and smooth Semen Gossypii protein isolate grinding slurry uniformly with colloid mill, add 280 kilograms of distilled water again, drop in the reaction filling, with molar concentration is that to regulate its pH value be 8.0 for the sodium hydroxide solution of 1mol/L, adding label under the state of continuous stirring is the alkaline protease 0.8kg of Alcalase, and be heated to 55 ℃ and be hydrolyzed, constantly sodium hydroxide solution adjustment and the maintenance pH value with 1mol/L is 7.9 in hydrolytic process, keeping temperature is 55 ℃, continues hydrolysis 1.5 hours.After hydrolysis for the first time finishes, with molar concentration be the hydrochloric acid solution of 1mol/L to adjust its pH value be 7.1, thermoregulation is 49 ℃, the adding label is Flavourzyme under the state that constantly stirs
TMFlavor protease 0.8kg, constantly sodium hydroxide solution adjustment and the maintenance pH value with 1mol/L is 7.1 in hydrolytic process, and keeping temperature is 49 ℃, continues hydrolysis 3 hours, after hydrolysis finishes, the heated and boiled hydrolyzed solution made enzyme-deactivating in 20 minutes, opened reaction then and irritated outer condensed water, made hydrolyzed solution be cooled to 70 ℃, emitting hydrolyzed solution to filter pocket filters, gained filtrate vacuum concentration is spray dried to 100 purpose fine powders to 43Brix with concentrated solution, promptly gets the cotton-seed polypeptide dry powder with antioxidation.
Claims (6)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100905875A CN100506276C (en) | 2006-06-29 | 2006-06-29 | Production method of cottonseed polypeptide dry powder with antioxidant effect |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100905875A CN100506276C (en) | 2006-06-29 | 2006-06-29 | Production method of cottonseed polypeptide dry powder with antioxidant effect |
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| Publication Number | Publication Date |
|---|---|
| CN1895661A true CN1895661A (en) | 2007-01-17 |
| CN100506276C CN100506276C (en) | 2009-07-01 |
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| CNB2006100905875A Expired - Fee Related CN100506276C (en) | 2006-06-29 | 2006-06-29 | Production method of cottonseed polypeptide dry powder with antioxidant effect |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101589761B (en) * | 2008-05-29 | 2011-10-26 | 中国人民解放军总后勤部军需装备研究所 | Preparation method and application of industrial hemp seed antioxidant peptide |
| CN102898504A (en) * | 2012-10-23 | 2013-01-30 | 中国农业大学 | Antioxidation active synthetic peptide and purpose thereof |
| CN102898503A (en) * | 2012-10-23 | 2013-01-30 | 中国农业大学 | Antioxidation-activity synthetic peptides and application thereof |
| CN104450846A (en) * | 2014-12-05 | 2015-03-25 | 中南林业科技大学 | Semi-continuous preparation method of antihypertensive peptide of oil-tea meal protein |
| CN104531814A (en) * | 2014-12-05 | 2015-04-22 | 中南林业科技大学 | Preparation method of oil-tea meal enzymatically-modified protein |
| CN104593319A (en) * | 2013-10-31 | 2015-05-06 | 中国食品发酵工业研究院 | A wheat bioactive peptide additive used for a cell culture medium |
-
2006
- 2006-06-29 CN CNB2006100905875A patent/CN100506276C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101589761B (en) * | 2008-05-29 | 2011-10-26 | 中国人民解放军总后勤部军需装备研究所 | Preparation method and application of industrial hemp seed antioxidant peptide |
| CN102898504A (en) * | 2012-10-23 | 2013-01-30 | 中国农业大学 | Antioxidation active synthetic peptide and purpose thereof |
| CN102898503A (en) * | 2012-10-23 | 2013-01-30 | 中国农业大学 | Antioxidation-activity synthetic peptides and application thereof |
| CN102898504B (en) * | 2012-10-23 | 2014-08-06 | 中国农业大学 | Antioxidation active synthetic peptide and purpose thereof |
| CN104593319A (en) * | 2013-10-31 | 2015-05-06 | 中国食品发酵工业研究院 | A wheat bioactive peptide additive used for a cell culture medium |
| CN104450846A (en) * | 2014-12-05 | 2015-03-25 | 中南林业科技大学 | Semi-continuous preparation method of antihypertensive peptide of oil-tea meal protein |
| CN104531814A (en) * | 2014-12-05 | 2015-04-22 | 中南林业科技大学 | Preparation method of oil-tea meal enzymatically-modified protein |
| CN104531814B (en) * | 2014-12-05 | 2017-11-14 | 中南林业科技大学 | A kind of preparation method of Extracted From Oil-tea-cake enzyme modification albumen |
| CN104450846B (en) * | 2014-12-05 | 2018-05-01 | 中南林业科技大学 | A kind of Extracted From Oil-tea-cake protein blood pressure-reducing peptide semi continuous preparation method |
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| CN100506276C (en) | 2009-07-01 |
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