CN101096697B - Industrial production method of ovum protein polypeptide from fowl ovum by enzymatical process - Google Patents
Industrial production method of ovum protein polypeptide from fowl ovum by enzymatical process Download PDFInfo
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- CN101096697B CN101096697B CN200710016177A CN200710016177A CN101096697B CN 101096697 B CN101096697 B CN 101096697B CN 200710016177 A CN200710016177 A CN 200710016177A CN 200710016177 A CN200710016177 A CN 200710016177A CN 101096697 B CN101096697 B CN 101096697B
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- ovum
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- egg white
- enzymolysis
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 15
- 102000002322 Egg Proteins Human genes 0.000 title claims description 50
- 108010000912 Egg Proteins Proteins 0.000 title claims description 50
- 229920001184 polypeptide Polymers 0.000 title claims description 20
- 102000004196 processed proteins & peptides Human genes 0.000 title claims description 20
- 210000004681 ovum Anatomy 0.000 title claims description 19
- 238000009776 industrial production Methods 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 claims abstract description 13
- 239000000843 powder Substances 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims abstract description 5
- 238000005507 spraying Methods 0.000 claims abstract description 5
- 239000000047 product Substances 0.000 claims description 14
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 13
- 235000014103 egg white Nutrition 0.000 claims description 13
- 210000000969 egg white Anatomy 0.000 claims description 13
- 102000004190 Enzymes Human genes 0.000 claims description 12
- 108090000790 Enzymes Proteins 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 102000004169 proteins and genes Human genes 0.000 claims description 10
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 238000010792 warming Methods 0.000 claims description 6
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 5
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 5
- 239000000920 calcium hydroxide Substances 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 3
- 238000009413 insulation Methods 0.000 claims description 3
- 238000005374 membrane filtration Methods 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- 235000013601 eggs Nutrition 0.000 abstract description 5
- 235000013305 food Nutrition 0.000 abstract description 4
- 108091005804 Peptidases Proteins 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 108010058846 Ovalbumin Proteins 0.000 abstract 1
- 102000035195 Peptidases Human genes 0.000 abstract 1
- 239000002131 composite material Substances 0.000 abstract 1
- 239000002537 cosmetic Substances 0.000 abstract 1
- 235000019833 protease Nutrition 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 241000271566 Aves Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000001641 gel filtration chromatography Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 229940116540 protein supplement Drugs 0.000 description 1
- 235000005974 protein supplement Nutrition 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a unique extracting method of egg albumin from eggs, which comprises the following steps: using composite proteinase to enzymolyze egg; separating; hyperfiltering; condensing; spraying; drying; obtaining the white powder shaped product. The invention simplifies the technique and shortens the manufacturing period with good taste and small molecular weight of hybrid peptide, which can be clinical nutrition, hygienic food, sports food and cosmetics.
Description
(1) technical field
The present invention relates to a kind of highly purified ovum protein polypeptide industrialization product, particularly a kind of from the fowl ovum industrial process of ovum by enzymatical process protein polypeptide.
(2) background technology
Contain a certain amount of protein in the fowl ovum, but be difficult to be absorbed by organism, the peptide class is by the total free aminoacids of organism absorption rate faster than equivalent, more far away faster than the protein of equivalent.The fowl ovum is produced the ovum protein polypeptide can better be digested and assimilated.At present, the method for producing the ovum protein polypeptide from the fowl ovum mainly contains acid hydrolyzation and enzymolysis process, and the acid hydrolyzation reaction is violent, reaction process and final product are difficult to control, can cause a large amount of amino acid whose destructions, with fowl ovum direct enzymolysis, difficulty is big, the time is long, be difficult for filtration, to obtain the peptide rate low.
(3) summary of the invention
The present invention is in order to remedy the deficiencies in the prior art, provides a kind of technology the industrial process of ovum by enzymatical process protein polypeptide from the fowl ovum simple, with short production cycle.
The present invention is achieved through the following technical solutions:
A kind of from the fowl ovum protein industrial process of ovum by enzymatical process protein polypeptide, its special character is: may further comprise the steps:
(1) with 10kg fowl egg white wash clean, puts steam cooker and boil, seethe with excitement after 15-20 minute, the fowl ovum is lowered the temperature with cold water flush, peel off that to get its egg white standby;
(2) sex change egg white is added deionized water 8500-9500ml, fully grind, cross the 40-60 mesh sieve with shredder or colloidal mill;
(3) the egg white aqueous solution is warming up to 50-55 ℃, and to transfer pH value with 30% calcium hydroxide be 7.0-7.4, papoid 200-300g enzyme powder adds in the feed liquid with deionized water 500-700ml dissolving back, and enzymolysis begins; Stir, keep 50-55 ℃ of pH value 7.0-7.4 temperature during the enzymolysis;
(4) the enzymolysis enzyme that goes out after 5 hours is warming up to boiling state simultaneously and kept 5 minutes;
(5) go out and use the 0.5um membrane filtration behind the enzyme, collect filtrate;
(6) filtrate enters vacuum concentration pot and concentrates concentration 20% discharging;
(7) add 8-10% activated carbon 400-500g, Plate Filtration is carried out in the 80 ℃ of insulations 0.5 hour that heat up;
(8) feed liquid is carried out spraying drying, cross 60 mesh sieves, product yield 45-50%; The laminated bag packing.
Of the present invention from the fowl ovum protein industrial process of ovum by enzymatical process protein polypeptide, described deionized water pH value 7.5-10, specific conductivity≤50us/cm; Papoid enzyme activity 1,000,000 u/g.
The present invention for a kind of uniqueness, from the fowl ovum, extract ovum protein, use compound protease enzymolysis ovum protein then, the production technique of separating purification ovum protein polypeptide, be raw material mainly with the egg white powder, through enzymolysis, separation, ultrafiltration, concentrated, spraying drying, make a kind of white powder ovum protein polypeptide.The present invention is on the basis of daring to experiment, carefully solving, and has confirmed to use the high purity separation proteolysis, and thorough enzymolysis, time lack, very easily filter, obtain peptide rate height, have obtained the effect better than former anticipation, have improved the scientific and technological content and the quality of product greatly.The applicant's innovation makes the product of enterprise become the crystallization of protein engineering and two science common factors of biotechnology, becomes two degree high-tech deep processed products, and its scientific and technological content more is higher than general polypeptide.
Protein content of the present invention is more than 85%, and the molecular weight of 90% ovum protein polypeptide is below 1000Dalton.Fowl ovum protein of the present invention is produced the ovum protein polypeptide by limited ground enzymatic hydrolysis.Technology of the present invention is simple, with short production cycle, the ovum protein polypeptide products white of being produced, no fishy smell, mouthfeel is good, contained mixed peptide molecular weight is little, can be used as the nutrient protein supplement and directly eats, and also can be used as the function batching of clinical nutrition product, protective foods, motion food and makeup, for improving the birds, beasts and eggs value of the product, the product of production high value has found a kind of new approach.
(4) description of drawings
The present invention is further illustrated below in conjunction with accompanying drawing.
Fig. 1 is a process flow diagram of the present invention;
Fig. 2 is the graph of molecular weight distribution of products obtained therefrom ovum protein polypeptide of the present invention;
Fig. 3 is the graph of molecular weight distribution of products obtained therefrom ovum protein polypeptide of the present invention.
(5) embodiment
Accompanying drawing is a kind of specific embodiment of the present invention.As shown in Figure 1, this embodiment comprises the steps:
1. with 10kg Ovum Gallus domesticus album wash clean, put steam cooker and boil, seethe with excitement after 15-20 minute, egg is lowered the temperature with cold water flush, peel off that to get its egg white standby.Egg white weight is 5500-6000g.
2. (pH value 7.5-10, specific conductivity≤50us/cm), fully grind with shredder or colloidal mill are crossed the 40-60 mesh sieve sex change egg white to be added deionized water 8500-9500ml.
3. the egg white aqueous solution is warming up to 50-55 ℃, and to transfer pH value with 30% calcium hydroxide 5.5-6.5ml be 7.0-7.4, add papoid 200-300g (enzyme activity 1,000,000 u/g), the enzyme powder adds in the feed liquid with deionized water 500-700ml dissolving back, and enzymolysis begins.Temperature stirs in this process, 0.5 hour pH value 6.5-6.9 of enzymolysis, adding calcium hydroxide 15-20ml, pH value 7.0-7.4,1.5 hour pH value 6.5-6.9, adding calcium hydroxide 10-15ml, pH value 7.0-7.4,2.5 hours pH value 7.0-7.4,3.5 hour pH value 7.0-7.4,4.5 hours pH value 7.0-7.4.(keeping 50-55 ℃ of pH value 7.0-7.4 temperature during the enzymolysis at any time)
4. the hydrolysis enzyme that goes out after 5 hours is warming up to boiling state simultaneously and kept 5 minutes.
5. go out and use the 0.5um membrane filtration behind the enzyme, collect filtrate 6500-7000ml.
6. filtrate enters vacuum concentration pot and concentrates concentration 20% discharging 2500-3000ml.
7. add 8-10% activated carbon 400-500g, Plate Filtration is carried out in the 80 ℃ of insulations 0.5 hour that heat up.
8. feed liquid is carried out spraying drying, cross 60 mesh sieves, product yield 45-50%.The laminated bag packing.
The molecular weight distribution high performance gel filtration chromatography assay determination of products obtained therefrom egg albumen polypeptide according to the present invention, graph of molecular weight distribution as shown in Figure 2, the molecular weight distribution table is as shown in Figure 3.
Claims (2)
1. the industrial process of an ovum by enzymatical process protein polypeptide from the fowl ovum protein is characterized in that: may further comprise the steps:
(1) with 10kg fowl egg white wash clean, puts steam cooker and boil, seethe with excitement after 15-20 minute, the fowl ovum is lowered the temperature with cold water flush, peel off that to get its egg white standby;
(2) sex change egg white is added deionized water 8500-9500ml, fully grind, cross the 40-60 mesh sieve with shredder;
(3) the egg white aqueous solution is warming up to 50-55 ℃, and to transfer pH value with 30% calcium hydroxide be 7.0-7.4, papoid 200-300g, the enzyme powder adds in the feed liquid with deionized water 500-700ml dissolving back, and enzymolysis begins; Stir, keep pH value 7.0-7.4, temperature 50-55 ℃ during the enzymolysis;
(4) the enzymolysis enzyme that goes out after 5 hours is warming up to boiling state simultaneously and kept 5 minutes;
(5) go out and use the 0.5um membrane filtration behind the enzyme, collect filtrate;
(6) filtrate enters vacuum concentration pot and concentrates concentration 20% discharging;
(7) add 8-10% activated carbon 400-500g, Plate Filtration is carried out in the 80 ℃ of insulations 0.5 hour that heat up;
(8) feed liquid is carried out spraying drying, cross 60 mesh sieves, product yield 45-50%; The laminated bag packing.
2. according to claim 1 from the fowl ovum protein industrial process of ovum by enzymatical process protein polypeptide, it is characterized in that: described deionized water pH value 7.5-10, specific conductivity≤50us/cm; Papoid enzyme activity 1,000,000 u/g.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200710016177A CN101096697B (en) | 2007-07-06 | 2007-07-06 | Industrial production method of ovum protein polypeptide from fowl ovum by enzymatical process |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710016177A CN101096697B (en) | 2007-07-06 | 2007-07-06 | Industrial production method of ovum protein polypeptide from fowl ovum by enzymatical process |
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| CN101096697A CN101096697A (en) | 2008-01-02 |
| CN101096697B true CN101096697B (en) | 2010-05-19 |
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| CN200710016177A Active CN101096697B (en) | 2007-07-06 | 2007-07-06 | Industrial production method of ovum protein polypeptide from fowl ovum by enzymatical process |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974597B (en) * | 2010-11-11 | 2012-07-04 | 湖北远成药业有限公司 | Method for extracting ovalbumin peptide |
| CN102787154B (en) * | 2011-05-20 | 2013-11-06 | 中国食品发酵工业研究院 | Preparation method of black-bone chicken oligopeptide and separation and identification method of active peptide fragment |
| CN102978268A (en) * | 2012-12-26 | 2013-03-20 | 山东天智绿业生物科技有限公司 | Method for preparing egg albumin polypeptide from egg albumin powder by enzymic method |
| CN104480175B (en) * | 2014-07-22 | 2018-02-27 | 上海荣神生物化学有限公司 | A kind of method of enzyme-hydrolysis albumen and the application of enzymolysis product |
| CN104758207B (en) * | 2015-03-16 | 2018-01-23 | 佛山市万盈化妆品有限公司 | One kind improves black-eyed eye essence |
| CN106031436A (en) * | 2015-03-21 | 2016-10-19 | 山东中泰药业有限公司 | Nutritional preparation containing egg white and preparation method thereof |
| CN105475881A (en) * | 2015-10-15 | 2016-04-13 | 益阳陈克明食品股份有限公司 | Desalination method for salted egg white, salted-egg-white protein peptide powder and preparation method |
| CN109845878A (en) * | 2018-11-08 | 2019-06-07 | 武汉森澜生物科技有限公司 | A kind of compound protein peptide of strengthen immunity and its application |
| CN111616371A (en) * | 2019-12-26 | 2020-09-04 | 长春大学 | A kind of preparation method of producing non-digestible protein by using various ovalbumin as raw material |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1175360A (en) * | 1996-09-01 | 1998-03-11 | 魏剑鸣 | Egg albumin zymolysis matter and preparing process thereof |
| CN1359630A (en) * | 2001-08-13 | 2002-07-24 | 邹远东 | Process for preparing protein powder by enzymolyzing whole egg with papainase and use of said protein powder |
-
2007
- 2007-07-06 CN CN200710016177A patent/CN101096697B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1175360A (en) * | 1996-09-01 | 1998-03-11 | 魏剑鸣 | Egg albumin zymolysis matter and preparing process thereof |
| CN1359630A (en) * | 2001-08-13 | 2002-07-24 | 邹远东 | Process for preparing protein powder by enzymolyzing whole egg with papainase and use of said protein powder |
Non-Patent Citations (2)
| Title |
|---|
| 刘静波等.外切蛋白酶作用蛋清蛋白制备高F 值寡肽的外切效果研究.食品科学27 11.2006,27(11),第192页1.3方法，第194页表4和表6. |
| 刘静波等.外切蛋白酶作用蛋清蛋白制备高F 值寡肽的外切效果研究.食品科学27 11.2006,27(11),第192页1.3方法,第194页表4和表6. * |
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Effective date of registration: 20201214 Address after: 274000 Chenji Industrial Zone, Heze Development Zone, Shandong Province Patentee after: ZHONGSHI DUQING (SHANDONG) BIOTECH Co.,Ltd. Address before: 274108 Chen Ji industrial zone, Dingtao County, Shandong, Heze Patentee before: SHANDONG TIANJIU BIOTECHNOLOGY Co.,Ltd. |
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