CN102978268A - Method for preparing egg albumin polypeptide from egg albumin powder by enzymic method - Google Patents
Method for preparing egg albumin polypeptide from egg albumin powder by enzymic method Download PDFInfo
- Publication number
- CN102978268A CN102978268A CN2012105711443A CN201210571144A CN102978268A CN 102978268 A CN102978268 A CN 102978268A CN 2012105711443 A CN2012105711443 A CN 2012105711443A CN 201210571144 A CN201210571144 A CN 201210571144A CN 102978268 A CN102978268 A CN 102978268A
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- China
- Prior art keywords
- ovum
- egg albumin
- protein powder
- albumin polypeptide
- filtrate
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- 238000000034 method Methods 0.000 title claims abstract description 30
- 239000000843 powder Substances 0.000 title claims abstract description 24
- 108010058846 Ovalbumin Proteins 0.000 title abstract 9
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 26
- 239000000706 filtrate Substances 0.000 claims abstract description 11
- 239000000047 product Substances 0.000 claims abstract description 11
- 102000004190 Enzymes Human genes 0.000 claims abstract description 10
- 108090000790 Enzymes Proteins 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000008367 deionised water Substances 0.000 claims abstract description 9
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 9
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 230000000694 effects Effects 0.000 claims abstract description 6
- 230000007062 hydrolysis Effects 0.000 claims abstract description 6
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 6
- 238000005374 membrane filtration Methods 0.000 claims abstract description 6
- 238000009835 boiling Methods 0.000 claims abstract description 4
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 32
- 108010000912 Egg Proteins Proteins 0.000 claims description 32
- 229920001184 polypeptide Polymers 0.000 claims description 16
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 16
- 102000009027 Albumins Human genes 0.000 claims description 11
- 108010088751 Albumins Proteins 0.000 claims description 11
- 210000004681 ovum Anatomy 0.000 claims description 11
- 238000009413 insulation Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000010792 warming Methods 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 3
- 239000004365 Protease Substances 0.000 abstract description 2
- 229910052799 carbon Inorganic materials 0.000 abstract description 2
- 230000009849 deactivation Effects 0.000 abstract description 2
- 230000035484 reaction time Effects 0.000 abstract description 2
- 229940088598 enzyme Drugs 0.000 abstract 2
- 238000010438 heat treatment Methods 0.000 abstract 2
- 108090000526 Papain Proteins 0.000 abstract 1
- 229940055729 papain Drugs 0.000 abstract 1
- 235000019834 papain Nutrition 0.000 abstract 1
- 238000001694 spray drying Methods 0.000 abstract 1
- 239000002253 acid Substances 0.000 description 6
- 150000001413 amino acids Chemical group 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 108010016626 Dipeptides Proteins 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 229940116540 protein supplement Drugs 0.000 description 1
- 235000005974 protein supplement Nutrition 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention relates to the field of production of high-purity egg albumin polypeptide, and especially discloses a method for preparing egg albumin polypeptide from egg albumin powder by an enzymic method. According to the method for preparing egg albumin polypeptide from egg albumin powder by an enzymic method, the egg albumin powder is used as raw material; and the method provided by the invention is characterized by comprising the following steps of: dissolving the egg albumin powder in deionized water, adding a papain solution for hydrolysis, then, heating up the solution to be boiling, preserving heat and deactivating enzyme; executing membrane filtration to collect filtrate from the solution treated by enzyme deactivation; concentrating the filtrate in a vacuum low-temperature triple effect concentrating pan, and then, adding the active carbon, heating up, preserving heat and executing frame filtering, and carrying out spray drying for the filtrate to get the finished product. The method provided by the invention is simple in steps, low in production cost, exhaustive in enzymolysis, short in reaction time, easy to filter, high in peptide yield and capable of greatly improving the technological content and the quality of products, and being used and popularized widely.
Description
(1) technical field
The present invention relates to high purity Protalbinic acid polypeptide production field, particularly a kind of from the ovum protein powder method of ovum by enzymatical process albumin polypeptide.
(2) background technology
Protein peptide is a kind of biologically active substance, is that albumen is through the prepared mixing oligopeptide with some biological function of the methods such as protease hydrolysis or microbial fermentation.From chemical terms, protein peptide then is that 20 natural amino acids form with difference and the general name of the different peptide classes of the linearity from the dipeptides to the complexity of arrangement mode formation, ring structure in the protein.Biological test shows that the protein that body is taken in absorbs with the low molecular weight peptide form mostly after gastral enzyme effect, the ratio that absorbs with the total free aminoacids form is very little.Further test disclosed again the digestion of low molecular weight peptide specific ionization amino acid faster, absorb manyly, above-mentioned result of study all biological value and the nutritive value specific ionization amino acid of surperficial low molecular weight peptide is higher.
At present, the Protalbinic acid polypeptide generally obtains by the direct enzymolysis of ovum protein liquid, and the production process difficulty is large, the time is long, should not filter, and it is low to obtain the peptide rate.
(3) summary of the invention
The present invention is in order to remedy the deficiencies in the prior art, the method for ovum by enzymatical process albumin polypeptide from the ovum protein powder that provides that a kind of step is simple, production cost is low.
The present invention is achieved through the following technical solutions:
A kind of from the ovum protein powder method of ovum by enzymatical process albumin polypeptide, take the ovum protein powder as raw material, it is characterized in that: the ovum protein powder is dissolved in the deionized water, add papoid solution, be the hydrolysis in 8 hours of 7 ± 1 times stirring reactions in 40 ± 1 ℃, pH, then be warming up to after the boiling insulation 5min enzyme that goes out; The solution that will go out behind the enzyme is collected filtrate by membrane filtration; Place vacuum and low temperature triple effect concentrating pan concentrated filtrate, then add the gac of concentrated solution quality 8 ~ 10%, be warming up to 75 ℃ after insulation carry out Plate Filtration, filtrate is carried out spraying drying obtains finished product.
The present invention adopts this natural active substance of ovum protein powder, and its molecular weight is larger, is difficult for directly absorbed by the body, be hydrolyzed into a kind of polypeptide and amino acid whose Protalbinic acid peptide product that easily is absorbed by the body that contain, cost is low, has no side effect, but as the nutritive food widespread use.
More excellent scheme of the present invention is:
The quality proportioning of described ovum protein powder and deionized water is 1:2, and papoid solution is that every 1g papoid adds the 1kg deionized water.
The film that adopts during described membrane filtration is 0.3 μ m aperture.
The mass concentration of described concentrated solution is 20%.
Under 75 ℃, be incubated 0.2 hour before the described Plate Filtration.
Particle is crossed 60 mesh sieves after the described spraying drying.
Its protein content of product of the present invention is more than 85%, the molecular weight of 90% Protalbinic acid polypeptide is below 1000 Dalton, also be designed into simultaneously the limited enzymatic hydrolysis of ovum protein pruinescence, produce the Protalbinic acid polypeptide, it is directly edible to can be used as the nutrient protein supplement, also can be used as the function raw material of clinical nutrition product, protective foods, sport video and makeup, be worth for improving the ovum egg product, the product of production high value has found a kind of new approach.
Step of the present invention is simple, and production cost is low, thorough enzymolysis, and the reaction times is short, very easily filters, and it is high to obtain the peptide rate, has greatly improved scientific and technological content and the quality of product, uses wide popularization and application.
(4) embodiment
Embodiment:
Its processing step is:
Ovum protein powder → add water adjust pH → add enzymic hydrolysis → intensification enzyme → filtration → vacuum concentration → cryodrying → Protalbinic acid polypeptide that goes out of sizing mixing → heat.
Its concrete operation step is:
(1) 5kg ovum protein powder is dissolved in the 10kg deionized water, and constantly stirs;
(2) get papoid 10g(enzyme activity 1,000,000 u/g), add the 10kg deionized water, add after the dissolving in the feed liquid, stirring fully mixes; Temperature stirs in this process, and temperature is controlled at positive and negative 1 degree of 40 degree, and PH was 7 ± 1 reactions 8 hours;
(3) hydrolysis stopped reaction after 8 hours, enzymolysis is complete, is warming up to simultaneously boiling state and keeps 5 minutes, fully deactivation;
(4) go out and use the 0.3um membrane filtration behind the enzyme, elimination is the albumin of enzymolysis, collects filtrate 10000 ~ 11000ml;
(5) filtrate enters vacuum and low temperature triple effect concentrating pan and concentrates concentration 20% discharging 2500 ~ 3000ml;
(6) add 8-10% activated carbon 400-500g, Plate Filtration is carried out in the 75 ℃ of insulations 0.2 hour that heat up;
(7) feed liquid is carried out spraying drying, cross 60 mesh sieves, product yield 45-50%; The laminated bag packing.
Claims (6)
1. the method for an ovum by enzymatical process albumin polypeptide from the ovum protein powder, take the ovum protein powder as raw material, it is characterized in that: the ovum protein powder is dissolved in the deionized water, add papoid solution, be the hydrolysis in 8 hours of 7 ± 1 times stirring reactions in 40 ± 1 ℃, pH, then be warming up to after the boiling insulation 5min enzyme that goes out; The solution that will go out behind the enzyme is collected filtrate by membrane filtration; Place vacuum and low temperature triple effect concentrating pan concentrated filtrate, then add the gac of concentrated solution quality 8 ~ 10%, be warming up to 75 ℃ after insulation carry out Plate Filtration, filtrate is carried out spraying drying obtains finished product.
According to claim 1 from the ovum protein powder method of ovum by enzymatical process albumin polypeptide, it is characterized in that: the quality proportioning of described ovum protein powder and deionized water is 1:2, papoid solution is that every 1g papoid adds the 1kg deionized water.
According to claim 1 from the ovum protein powder method of ovum by enzymatical process albumin polypeptide, it is characterized in that: the film that adopts during described membrane filtration is 0.3 μ m aperture.
According to claim 1 from the ovum protein powder method of ovum by enzymatical process albumin polypeptide, it is characterized in that: the mass concentration of described concentrated solution is 20%.
According to claim 1 from the ovum protein powder method of ovum by enzymatical process albumin polypeptide, it is characterized in that: before the described Plate Filtration 75 ℃ of lower insulations 0.2 hour.
According to claim 1 from the ovum protein powder method of ovum by enzymatical process albumin polypeptide, it is characterized in that: particle is crossed 60 mesh sieves after the described spraying drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105711443A CN102978268A (en) | 2012-12-26 | 2012-12-26 | Method for preparing egg albumin polypeptide from egg albumin powder by enzymic method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105711443A CN102978268A (en) | 2012-12-26 | 2012-12-26 | Method for preparing egg albumin polypeptide from egg albumin powder by enzymic method |
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| Publication Number | Publication Date |
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| CN102978268A true CN102978268A (en) | 2013-03-20 |
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| CN2012105711443A Pending CN102978268A (en) | 2012-12-26 | 2012-12-26 | Method for preparing egg albumin polypeptide from egg albumin powder by enzymic method |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104480175A (en) * | 2014-07-22 | 2015-04-01 | 上海荣神生物化学有限公司 | Enzymolysis method of ovalbumin and application of enzymolysis products |
| CN109091409A (en) * | 2018-10-25 | 2018-12-28 | 福建农林大学 | A kind of preparation method of passionflower anthocyanidin and ovalbumin peptide facial mask dry powder |
| CN110771902A (en) * | 2019-11-21 | 2020-02-11 | 广东肽世家生物科技有限公司 | Composite polypeptide powder and application thereof |
| CN111631337A (en) * | 2020-05-30 | 2020-09-08 | 泉州玺阅日化有限公司 | Health effervescent tablet containing animal albumin and preparation method thereof |
| CN112250758A (en) * | 2020-09-21 | 2021-01-22 | 杨志伟 | Albumin stock solution, preparation method and application thereof |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| CN113462559A (en) * | 2021-07-26 | 2021-10-01 | 吴艳 | Promotion preparation system suitable for albumin polypeptide |
| US11160299B2 (en) | 2019-07-11 | 2021-11-02 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US11279748B2 (en) | 2014-11-11 | 2022-03-22 | Clara Foods Co. | Recombinant animal-free food compositions and methods of making them |
| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1101377A (en) * | 1993-10-07 | 1995-04-12 | 上海益人生化技术开发有限公司 | Enzymolytic egg albumen solution as health-care liquid |
| CN1175360A (en) * | 1996-09-01 | 1998-03-11 | 魏剑鸣 | Egg albumin zymolysis matter and preparing process thereof |
| CN101096697A (en) * | 2007-07-06 | 2008-01-02 | 山东天久生物技术有限公司 | Industrial production method of ovum protein polypeptide from fowl ovum by enzymatical process |
-
2012
- 2012-12-26 CN CN2012105711443A patent/CN102978268A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1101377A (en) * | 1993-10-07 | 1995-04-12 | 上海益人生化技术开发有限公司 | Enzymolytic egg albumen solution as health-care liquid |
| CN1175360A (en) * | 1996-09-01 | 1998-03-11 | 魏剑鸣 | Egg albumin zymolysis matter and preparing process thereof |
| CN101096697A (en) * | 2007-07-06 | 2008-01-02 | 山东天久生物技术有限公司 | Industrial production method of ovum protein polypeptide from fowl ovum by enzymatical process |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104480175B (en) * | 2014-07-22 | 2018-02-27 | 上海荣神生物化学有限公司 | A kind of method of enzyme-hydrolysis albumen and the application of enzymolysis product |
| CN104480175A (en) * | 2014-07-22 | 2015-04-01 | 上海荣神生物化学有限公司 | Enzymolysis method of ovalbumin and application of enzymolysis products |
| US11518797B2 (en) | 2014-11-11 | 2022-12-06 | Clara Foods Co. | Methods and compositions for egg white protein production |
| US11279748B2 (en) | 2014-11-11 | 2022-03-22 | Clara Foods Co. | Recombinant animal-free food compositions and methods of making them |
| CN109091409A (en) * | 2018-10-25 | 2018-12-28 | 福建农林大学 | A kind of preparation method of passionflower anthocyanidin and ovalbumin peptide facial mask dry powder |
| CN109091409B (en) * | 2018-10-25 | 2021-09-21 | 福建农林大学 | Preparation method of passion flower anthocyanin and egg albumin peptide mask dry powder |
| US11160299B2 (en) | 2019-07-11 | 2021-11-02 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US11974592B1 (en) | 2019-07-11 | 2024-05-07 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US11800887B2 (en) | 2019-07-11 | 2023-10-31 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US11142754B2 (en) | 2019-08-07 | 2021-10-12 | Clara Foods Co. | Compositions comprising digestive enzymes |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| US11649445B2 (en) | 2019-08-07 | 2023-05-16 | Clara Foods Co. | Compositions comprising digestive enzymes |
| CN110771902A (en) * | 2019-11-21 | 2020-02-11 | 广东肽世家生物科技有限公司 | Composite polypeptide powder and application thereof |
| CN111631337A (en) * | 2020-05-30 | 2020-09-08 | 泉州玺阅日化有限公司 | Health effervescent tablet containing animal albumin and preparation method thereof |
| CN112250758A (en) * | 2020-09-21 | 2021-01-22 | 杨志伟 | Albumin stock solution, preparation method and application thereof |
| CN113462559A (en) * | 2021-07-26 | 2021-10-01 | 吴艳 | Promotion preparation system suitable for albumin polypeptide |
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Application publication date: 20130320 |