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CL2023000238A1 - Generación de plantas haploides - Google Patents

Generación de plantas haploides

Info

Publication number
CL2023000238A1
CL2023000238A1 CL2023000238A CL2023000238A CL2023000238A1 CL 2023000238 A1 CL2023000238 A1 CL 2023000238A1 CL 2023000238 A CL2023000238 A CL 2023000238A CL 2023000238 A CL2023000238 A CL 2023000238A CL 2023000238 A1 CL2023000238 A1 CL 2023000238A1
Authority
CL
Chile
Prior art keywords
plants
present
haploid
transgenic
generation
Prior art date
Application number
CL2023000238A
Other languages
English (en)
Inventor
Houben Andreas
KARIMI-ASHIYANI Raheleh
Ishii Takayoshi
Stein Nils
KUMLEHM Jochen
BOLDUAN Christof
Breuer Frank
Kloiber-Maitz Monika
Niessen Markus
Ouzunova Milena
Schulz Britta
WIECKHORST Silke
Original Assignee
Kws Saat Se & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP14182719.6A external-priority patent/EP2989889B8/en
Priority claimed from EP14004389.4A external-priority patent/EP3037540A1/en
Application filed by Kws Saat Se & Co Kgaa filed Critical Kws Saat Se & Co Kgaa
Publication of CL2023000238A1 publication Critical patent/CL2023000238A1/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/06—Roots
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/20—Brassicaceae, e.g. canola, broccoli or rucola
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención se refiere a plantas no transgénicas y transgénicas, preferiblemente plantas de cultivo, que comprende una mutación que causa una alteración de la secuencia de aminoácido en el dominio CATD del centrómero histona H3 (CENH3), preferiblemente dentro del bucle1 o la a2-helice del dominio CATD, que tiene la actividad biológica de un inductor haploide. Además, la presente invención proporciona métodos para generar las plantas de la presente invención y plantas haploides y doble haploides obtenibles al cruzar las plantas de la presente invención con plantas de tipo silvestre como también método para facilitar el intercambio de citoplasma.
CL2023000238A 2014-08-28 2023-01-25 Generación de plantas haploides CL2023000238A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14182719.6A EP2989889B8 (en) 2014-08-28 2014-08-28 Generation of haploid plants
EP14004389.4A EP3037540A1 (en) 2014-12-23 2014-12-23 Haploid inducer

Publications (1)

Publication Number Publication Date
CL2023000238A1 true CL2023000238A1 (es) 2023-07-28

Family

ID=54147123

Family Applications (2)

Application Number Title Priority Date Filing Date
CL2017000465A CL2017000465A1 (es) 2014-08-28 2017-02-27 Generación de plantas haploides
CL2023000238A CL2023000238A1 (es) 2014-08-28 2023-01-25 Generación de plantas haploides

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CL2017000465A CL2017000465A1 (es) 2014-08-28 2017-02-27 Generación de plantas haploides

Country Status (14)

Country Link
US (2) US10993391B2 (es)
EP (1) EP3186381B1 (es)
CN (1) CN106998665B (es)
AU (1) AU2015309289B2 (es)
CA (1) CA2959566A1 (es)
CL (2) CL2017000465A1 (es)
DK (1) DK3186381T3 (es)
EA (1) EA037899B1 (es)
ES (1) ES2926055T3 (es)
HU (1) HUE059854T2 (es)
MX (1) MX378674B (es)
PL (1) PL3186381T3 (es)
UA (1) UA124293C2 (es)
WO (1) WO2016030019A1 (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3037540A1 (en) * 2014-12-23 2016-06-29 Kws Saat Se Haploid inducer
EP3262177A4 (en) * 2015-02-24 2018-08-08 The Regents of The University of California Haploid induction
WO2018035070A2 (en) * 2016-08-16 2018-02-22 Monsanto Technology Llc Compositions and methods for plant haploid induction
WO2019136417A2 (en) * 2018-01-08 2019-07-11 The Regents Of The University Of California Cenh3 deletion mutants
JP6614622B2 (ja) * 2018-04-17 2019-12-04 国立大学法人名古屋大学 植物ゲノム編集方法
CN108624547B (zh) * 2018-05-22 2021-08-13 天津农学院 一种分离单子叶植物保卫细胞的方法
WO2019234129A1 (en) 2018-06-05 2019-12-12 KWS SAAT SE & Co. KGaA Haploid induction with modified dna-repair
WO2019234132A1 (en) * 2018-06-05 2019-12-12 KWS SAAT SE & Co. KGaA Base editing in polymerase theta deficient plants
US20210403901A1 (en) * 2018-11-01 2021-12-30 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Targeted mutagenesis using base editors
EP3660164A1 (en) 2018-11-30 2020-06-03 KWS SAAT SE & Co. KGaA Screening of germplasm for desired polymorphisms by assays tested on synthetic dna
EP3689135A1 (en) * 2019-01-30 2020-08-05 KWS SAAT SE & Co. KGaA Haploid inducers
CN109997683B (zh) * 2019-04-29 2021-01-01 华南农业大学 一种基于单倍体诱导系的水稻双单倍体育种方法
EP3794939A1 (en) * 2019-09-23 2021-03-24 Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) Generation of haploids based on mutation of sad2
CN116782762B (zh) * 2020-05-29 2024-11-19 科沃施种子欧洲股份两合公司 植物单倍体诱导
WO2021252619A1 (en) * 2020-06-09 2021-12-16 University Of Georgia Research Foundation, Inc. Heterozygous cenh3 monocots and methods of use thereof for haploid induction and simultaneous genome editing
WO2022117024A1 (zh) * 2020-12-03 2022-06-09 中国农业大学 孤雌生殖单倍体诱导基因及其应用
CN113549617B (zh) * 2021-06-03 2023-06-30 南京农业大学 编辑不结球白菜CENH3基因的sgRNA及其CRISPR/Cas9载体和应用
WO2023225469A2 (en) * 2022-05-19 2023-11-23 Syngenta Crop Protection Ag Conferring cytoplasmic male sterility
CN116463348B (zh) * 2023-05-26 2024-05-14 中国农业科学院作物科学研究所 利用CRISPR/Cas9系统编辑玉米ZmCENH3基因的sg RNA及其应用
CN117305326B (zh) * 2023-11-29 2024-02-09 中国农业科学院蔬菜花卉研究所 青花菜BoCENH3基因及其在单倍体诱导中的应用
CN117296710B (zh) * 2023-11-29 2024-03-01 中国农业科学院蔬菜花卉研究所 一种快速创制细胞质雄性不育系的方法
CN117867011A (zh) * 2024-03-11 2024-04-12 四川省农业科学院作物研究所 一种fls1基因在控制紫叶油菜形成中的应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011044132A1 (en) 2009-10-06 2011-04-14 The Regents Of The University Of California Generation of haploid plants and improved plant breeding
WO2014110274A2 (en) * 2013-01-09 2014-07-17 Regents Of The University Of California A California Corporation Generation of haploid plants
EP3262177A4 (en) * 2015-02-24 2018-08-08 The Regents of The University of California Haploid induction

Also Published As

Publication number Publication date
EA201790463A1 (ru) 2017-07-31
CA2959566A1 (en) 2016-03-03
PL3186381T3 (pl) 2022-10-31
EA037899B1 (ru) 2021-06-03
US20210259178A1 (en) 2021-08-26
AU2015309289B2 (en) 2021-04-01
BR112017003555A2 (pt) 2017-12-12
MX2017002519A (es) 2017-09-05
US11895960B2 (en) 2024-02-13
EP3186381A1 (en) 2017-07-05
US20170280646A1 (en) 2017-10-05
HUE059854T2 (hu) 2023-01-28
EP3186381B1 (en) 2022-07-06
DK3186381T3 (da) 2022-09-12
UA124293C2 (uk) 2021-08-25
MX378674B (es) 2025-03-11
WO2016030019A1 (en) 2016-03-03
CN106998665A (zh) 2017-08-01
ES2926055T3 (es) 2022-10-21
US10993391B2 (en) 2021-05-04
AU2015309289A1 (en) 2017-03-16
CL2017000465A1 (es) 2017-10-06
CN106998665B (zh) 2024-02-27

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