CA2249061C - Protein production process - Google Patents

Protein production process Download PDF

Info

Publication number: CA2249061C
Authority: CA; Canada
Prior art keywords: carbon source; enzyme; repressive; sugar; promoter
Prior art date: 1997-10-01
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

CA002249061A

Other languages

English (en)

French (fr)

Other versions

CA2249061A1 (en

Inventor

Karsten Hellmuth

Rual Lopez-Ulibarri

Anne Francoise Mayer

Heinrich Winfried Schlieker

Adolphus Van Loon

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

DSM IP Assets BV

Original Assignee

DSM IP Assets BV

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1997-10-01

Filing date

1998-09-29

Publication date

2009-02-24

1998-09-29 Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV

1999-04-01 Publication of CA2249061A1 publication Critical patent/CA2249061A1/en

2009-02-24 Application granted granted Critical

2009-02-24 Publication of CA2249061C publication Critical patent/CA2249061C/en

2018-09-29 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

238000004519 manufacturing process Methods 0.000 title description 16
230000014616 translation Effects 0.000 title 1
238000000034 method Methods 0.000 claims abstract description 41
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 27
229910052799 carbon Inorganic materials 0.000 claims abstract description 27
108090000623 proteins and genes Proteins 0.000 claims abstract description 21
102000004169 proteins and genes Human genes 0.000 claims abstract description 20
230000001718 repressive effect Effects 0.000 claims abstract description 13
210000003527 eukaryotic cell Anatomy 0.000 claims abstract description 7
239000000758 substrate Substances 0.000 claims abstract description 6
238000002360 preparation method Methods 0.000 claims abstract description 3
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 28
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 27
239000008103 glucose Substances 0.000 claims description 26
108010011619 6-Phytase Proteins 0.000 claims description 18
229940085127 phytase Drugs 0.000 claims description 16
240000004808 Saccharomyces cerevisiae Species 0.000 claims description 15
241000320412 Ogataea angusta Species 0.000 claims description 13
102000004190 Enzymes Human genes 0.000 claims description 12
108090000790 Enzymes Proteins 0.000 claims description 12
229940088598 enzyme Drugs 0.000 claims description 12
108010025188 Alcohol oxidase Proteins 0.000 claims description 10
241000235648 Pichia Species 0.000 claims description 9
108090000698 Formate Dehydrogenases Proteins 0.000 claims description 8
230000014509 gene expression Effects 0.000 claims description 8
241000235058 Komagataella pastoris Species 0.000 claims description 6
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
210000004027 cell Anatomy 0.000 claims description 6
229910052760 oxygen Inorganic materials 0.000 claims description 6
239000001301 oxygen Substances 0.000 claims description 6
108091028043 Nucleic acid sequence Proteins 0.000 claims description 5
241000222120 Candida <Saccharomycetales> Species 0.000 claims description 4
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
229930006000 Sucrose Natural products 0.000 claims description 4
-1 alcohol Proteins 0.000 claims description 4
150000001875 compounds Chemical class 0.000 claims description 4
239000003262 industrial enzyme Substances 0.000 claims description 4
239000005720 sucrose Substances 0.000 claims description 4
108010059892 Cellulase Proteins 0.000 claims description 3
101710093617 Dihydroxyacetone synthase Proteins 0.000 claims description 3
108010067193 Formaldehyde transketolase Proteins 0.000 claims description 3
229930091371 Fructose Natural products 0.000 claims description 3
239000005715 Fructose Substances 0.000 claims description 3
RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 3
229940106157 cellulase Drugs 0.000 claims description 3
239000001913 cellulose Substances 0.000 claims description 3
229920002678 cellulose Polymers 0.000 claims description 3
PVFDPMYXCZLHKY-MLLWLMKGSA-M sodium [(1R,2R,4aR,8aS)-2-hydroxy-5-[(2E)-2-[(4S)-4-hydroxy-2-oxooxolan-3-ylidene]ethyl]-1,4a,6-trimethyl-2,3,4,7,8,8a-hexahydronaphthalen-1-yl]methyl sulfate Chemical compound [Na+].C([C@@H]1[C@](C)(COS([O-])(=O)=O)[C@H](O)CC[C@]11C)CC(C)=C1C\C=C1/[C@H](O)COC1=O PVFDPMYXCZLHKY-MLLWLMKGSA-M 0.000 claims description 3
OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 2
LJCNDNBULVLKSG-UHFFFAOYSA-N 2-aminoacetic acid;butane Chemical compound CCCC.CCCC.NCC(O)=O LJCNDNBULVLKSG-UHFFFAOYSA-N 0.000 claims description 2
239000004382 Amylase Substances 0.000 claims description 2
102000013142 Amylases Human genes 0.000 claims description 2
108010065511 Amylases Proteins 0.000 claims description 2
102000016938 Catalase Human genes 0.000 claims description 2
108010053835 Catalase Proteins 0.000 claims description 2
102000029816 Collagenase Human genes 0.000 claims description 2
108060005980 Collagenase Proteins 0.000 claims description 2
101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 2
239000004366 Glucose oxidase Substances 0.000 claims description 2
108010015776 Glucose oxidase Proteins 0.000 claims description 2
229920002527 Glycogen Polymers 0.000 claims description 2
108090001060 Lipase Proteins 0.000 claims description 2
239000004367 Lipase Substances 0.000 claims description 2
102000004882 Lipase Human genes 0.000 claims description 2
GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 2
108091005804 Peptidases Proteins 0.000 claims description 2
102000003992 Peroxidases Human genes 0.000 claims description 2
108010059820 Polygalacturonase Proteins 0.000 claims description 2
239000004365 Protease Substances 0.000 claims description 2
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 2
229920002472 Starch Polymers 0.000 claims description 2
235000019418 amylase Nutrition 0.000 claims description 2
108010051210 beta-Fructofuranosidase Proteins 0.000 claims description 2
GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 2
229940105657 catalase Drugs 0.000 claims description 2
229960002424 collagenase Drugs 0.000 claims description 2
239000008121 dextrose Substances 0.000 claims description 2
108010093305 exopolygalacturonase Proteins 0.000 claims description 2
235000021433 fructose syrup Nutrition 0.000 claims description 2
229940116332 glucose oxidase Drugs 0.000 claims description 2
235000019420 glucose oxidase Nutrition 0.000 claims description 2
229940096919 glycogen Drugs 0.000 claims description 2
239000001573 invertase Substances 0.000 claims description 2
235000011073 invertase Nutrition 0.000 claims description 2
235000019421 lipase Nutrition 0.000 claims description 2
229940040461 lipase Drugs 0.000 claims description 2
235000013379 molasses Nutrition 0.000 claims description 2
108040007629 peroxidase activity proteins Proteins 0.000 claims description 2
235000019419 proteases Nutrition 0.000 claims description 2
239000008107 starch Substances 0.000 claims description 2
235000019698 starch Nutrition 0.000 claims description 2
239000006188 syrup Substances 0.000 claims description 2
235000020357 syrup Nutrition 0.000 claims description 2
101100226950 Pichia angusta FMDH gene Proteins 0.000 claims 2
108090000854 Oxidoreductases Proteins 0.000 claims 1
102000004316 Oxidoreductases Human genes 0.000 claims 1
230000000694 effects Effects 0.000 claims 1
125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 1
239000001963 growth medium Substances 0.000 claims 1
230000037353 metabolic pathway Effects 0.000 claims 1
150000007523 nucleic acids Chemical group 0.000 claims 1
210000005253 yeast cell Anatomy 0.000 claims 1
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 78
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 8
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 8
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 8
XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 7
241001225321 Aspergillus fumigatus Species 0.000 description 6
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 6
238000009010 Bradford assay Methods 0.000 description 4
229910004619 Na2MoO4 Inorganic materials 0.000 description 4
LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 4
229960002685 biotin Drugs 0.000 description 4
235000020958 biotin Nutrition 0.000 description 4
239000011616 biotin Substances 0.000 description 4
ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 4
229910000366 copper(II) sulfate Inorganic materials 0.000 description 4
229910052943 magnesium sulfate Inorganic materials 0.000 description 4
235000019341 magnesium sulphate Nutrition 0.000 description 4
SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 4
229910000357 manganese(II) sulfate Inorganic materials 0.000 description 4
230000004060 metabolic process Effects 0.000 description 4
LGQLOGILCSXPEA-UHFFFAOYSA-L nickel sulfate Chemical compound [Ni+2].[O-]S([O-])(=O)=O LGQLOGILCSXPEA-UHFFFAOYSA-L 0.000 description 4
229910000363 nickel(II) sulfate Inorganic materials 0.000 description 4
238000011218 seed culture Methods 0.000 description 4
239000011780 sodium chloride Substances 0.000 description 4
235000015393 sodium molybdate Nutrition 0.000 description 4
239000011684 sodium molybdate Substances 0.000 description 4
TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 4
235000019157 thiamine Nutrition 0.000 description 4
239000011721 thiamine Substances 0.000 description 4
NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 4
229910000368 zinc sulfate Inorganic materials 0.000 description 4
239000011686 zinc sulphate Substances 0.000 description 4
235000009529 zinc sulphate Nutrition 0.000 description 4
229910019931 (NH4)2Fe(SO4)2 Inorganic materials 0.000 description 3
230000012010 growth Effects 0.000 description 3
239000002054 inoculum Substances 0.000 description 3
239000000203 mixture Substances 0.000 description 3
238000012366 Fed-batch cultivation Methods 0.000 description 2
102000007625 Hirudins Human genes 0.000 description 2
108010007267 Hirudins Proteins 0.000 description 2
239000007836 KH2PO4 Substances 0.000 description 2
102000012607 Thrombomodulin Human genes 0.000 description 2
108010079274 Thrombomodulin Proteins 0.000 description 2
238000005273 aeration Methods 0.000 description 2
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
229910052921 ammonium sulfate Inorganic materials 0.000 description 2
235000011130 ammonium sulphate Nutrition 0.000 description 2
230000003698 anagen phase Effects 0.000 description 2
239000006285 cell suspension Substances 0.000 description 2
238000011161 development Methods 0.000 description 2
230000018109 developmental process Effects 0.000 description 2
239000003814 drug Substances 0.000 description 2
229940006607 hirudin Drugs 0.000 description 2
WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 description 2
244000005700 microbiome Species 0.000 description 2
229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
235000019796 monopotassium phosphate Nutrition 0.000 description 2
GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 1
239000002518 antifoaming agent Substances 0.000 description 1
229940091771 aspergillus fumigatus Drugs 0.000 description 1
238000010923 batch production Methods 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
230000001925 catabolic effect Effects 0.000 description 1
238000012512 characterization method Methods 0.000 description 1
230000000052 comparative effect Effects 0.000 description 1
238000010924 continuous production Methods 0.000 description 1
229940079593 drug Drugs 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
238000012262 fermentative production Methods 0.000 description 1
238000012215 gene cloning Methods 0.000 description 1
230000002503 metabolic effect Effects 0.000 description 1
239000000047 product Substances 0.000 description 1
238000000746 purification Methods 0.000 description 1
238000012552 review Methods 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
230000028327 secretion Effects 0.000 description 1
238000012807 shake-flask culturing Methods 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
239000013595 supernatant sample Substances 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
238000012546 transfer Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins

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Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Virology (AREA)
Tropical Medicine & Parasitology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)

CA002249061A 1997-10-01 1998-09-29 Protein production process Expired - Lifetime CA2249061C (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP97117021		1997-10-01
EP97117021.2		1997-10-01

Publications (2)

Publication Number	Publication Date
CA2249061A1 CA2249061A1 (en)	1999-04-01
CA2249061C true CA2249061C (en)	2009-02-24

Family

ID=8227420

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002249061A Expired - Lifetime CA2249061C (en)	1997-10-01	1998-09-29	Protein production process

Country Status (12)

Country	Link
US (1)	US6204012B1 (pt)
EP (2)	EP0911416B1 (pt)
JP (1)	JP4162774B2 (pt)
KR (1)	KR100572426B1 (pt)
CN (1)	CN1215175C (pt)
AU (1)	AU748187B2 (pt)
BR (1)	BR9803807B1 (pt)
CA (1)	CA2249061C (pt)
DE (1)	DE69834533T2 (pt)
DK (1)	DK0911416T3 (pt)
ES (1)	ES2264181T3 (pt)
IN (1)	IN188151B (pt)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6451572B1 (en)	1998-06-25	2002-09-17	Cornell Research Foundation, Inc.	Overexpression of phytase genes in yeast systems
BR0009516B1 (pt) *	1999-03-31	2013-09-10		vetor recombinante e célula hospedeira microbiana.
US6841370B1 (en)	1999-11-18	2005-01-11	Cornell Research Foundation, Inc.	Site-directed mutagenesis of Escherichia coli phytase
CA2465202C (en)	2001-10-31	2014-01-21	Phytex, Llc	Phytase-containing animal food and method
KR100429331B1 (ko) *	2002-01-29	2004-04-29	재단법인 목암생명공학연구소	재조합 효모를 이용한 에스에이치엘케이엔-1 단백질의대량생산방법
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- 1998-09-29 IN IN2187MA1998 patent/IN188151B/en unknown
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- 1998-09-30 CN CNB98118992XA patent/CN1215175C/zh not_active Expired - Lifetime
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DE69834533D1 (de)	2006-06-22
BR9803807B1 (pt)	2010-11-03
EP0911416A2 (en)	1999-04-28
DK0911416T3 (da)	2006-08-14
CN1218112A (zh)	1999-06-02
CN1215175C (zh)	2005-08-17
EP0911416B1 (en)	2006-05-17
IN188151B (pt)	2002-08-24
ES2264181T3 (es)	2006-12-16
EP0911416A3 (en)	2000-01-19
CA2249061A1 (en)	1999-04-01
JPH11169173A (ja)	1999-06-29
DE69834533T2 (de)	2006-10-12
BR9803807A (pt)	2000-03-28
JP4162774B2 (ja)	2008-10-08
AU8715798A (en)	1999-04-22
EP1659173A1 (en)	2006-05-24
AU748187B2 (en)	2002-05-30
KR19990036734A (ko)	1999-05-25
KR100572426B1 (ko)	2006-06-21
US6204012B1 (en)	2001-03-20

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