CA1116518A - Methode pour purifier une suspension de liposomes - Google Patents

Methode pour purifier une suspension de liposomes

Info

Publication number: CA1116518A
Authority: CA; Canada
Prior art keywords: resin; suspension; purified; lipidic; liposomic
Prior art date: 1977-09-30
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA000312035A

Other languages

English (en)

Inventor

Guido Neri

Alessandro Rigamonti

Luigi Moro

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Pfizer Italia SRL

Original Assignee

Farmaceutici Italia SpA

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1977-09-30

Filing date

1978-09-25

Publication date

1982-01-19

1978-09-25 Application filed by Farmaceutici Italia SpA filed Critical Farmaceutici Italia SpA

1982-01-19 Application granted granted Critical

1982-01-19 Publication of CA1116518A publication Critical patent/CA1116518A/fr

Status Expired legal-status Critical Current

Links

239000000725 suspension Substances 0.000 title claims abstract description 26
238000000034 method Methods 0.000 title claims abstract description 25
239000011347 resin Substances 0.000 claims abstract description 22
229920005989 resin Polymers 0.000 claims abstract description 22
229940079593 drug Drugs 0.000 claims abstract description 14
239000003814 drug Substances 0.000 claims abstract description 14
NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract description 4
239000003463 adsorbent Substances 0.000 claims abstract description 3
125000002091 cationic group Chemical group 0.000 claims abstract 3
HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 8
238000001914 filtration Methods 0.000 claims description 7
239000011521 glass Substances 0.000 claims description 7
150000003431 steroids Chemical class 0.000 claims description 5
239000004094 surface-active agent Substances 0.000 claims description 5
235000012000 cholesterol Nutrition 0.000 claims description 4
150000003904 phospholipids Chemical class 0.000 claims description 4
RNPXCFINMKSQPQ-UHFFFAOYSA-N dicetyl hydrogen phosphate Chemical compound CCCCCCCCCCCCCCCCOP(O)(=O)OCCCCCCCCCCCCCCCC RNPXCFINMKSQPQ-UHFFFAOYSA-N 0.000 claims description 3
229940093541 dicetylphosphate Drugs 0.000 claims description 3
239000000463 material Substances 0.000 claims description 3
PORPENFLTBBHSG-MGBGTMOVSA-N 1,2-dihexadecanoyl-sn-glycerol-3-phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(O)=O)OC(=O)CCCCCCCCCCCCCCC PORPENFLTBBHSG-MGBGTMOVSA-N 0.000 claims description 2
IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 2
229940067606 lecithin Drugs 0.000 claims description 2
235000010445 lecithin Nutrition 0.000 claims description 2
239000000787 lecithin Substances 0.000 claims description 2
150000002632 lipids Chemical class 0.000 claims description 2
239000000126 substance Substances 0.000 claims description 2
150000001875 compounds Chemical class 0.000 claims 3
229940067631 phospholipid Drugs 0.000 claims 3
125000000129 anionic group Chemical group 0.000 claims 2
239000013543 active substance Substances 0.000 claims 1
239000003456 ion exchange resin Substances 0.000 abstract description 3
229920003303 ion-exchange polymer Polymers 0.000 abstract description 3
230000000087 stabilizing effect Effects 0.000 abstract 1
239000002502 liposome Substances 0.000 description 17
AOJJSUZBOXZQNB-TZSSRYMLSA-N Doxorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 AOJJSUZBOXZQNB-TZSSRYMLSA-N 0.000 description 10
238000004108 freeze drying Methods 0.000 description 6
239000000243 solution Substances 0.000 description 6
229940009456 adriamycin Drugs 0.000 description 5
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
239000002952 polymeric resin Substances 0.000 description 4
229920002684 Sepharose Polymers 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
229920003002 synthetic resin Polymers 0.000 description 3
MWWSFMDVAYGXBV-RUELKSSGSA-N Doxorubicin hydrochloride Chemical compound Cl.O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(=O)CO)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 MWWSFMDVAYGXBV-RUELKSSGSA-N 0.000 description 2
GHASVSINZRGABV-UHFFFAOYSA-N Fluorouracil Chemical compound FC1=CNC(=O)NC1=O GHASVSINZRGABV-UHFFFAOYSA-N 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical group C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
REYJJPSVUYRZGE-UHFFFAOYSA-N Octadecylamine Chemical compound CCCCCCCCCCCCCCCCCCN REYJJPSVUYRZGE-UHFFFAOYSA-N 0.000 description 2
229910019142 PO4 Inorganic materials 0.000 description 2
238000004587 chromatography analysis Methods 0.000 description 2
239000008344 egg yolk phospholipid Substances 0.000 description 2
229960002949 fluorouracil Drugs 0.000 description 2
239000002808 molecular sieve Substances 0.000 description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
239000010452 phosphate Substances 0.000 description 2
229920001467 poly(styrenesulfonates) Polymers 0.000 description 2
239000011148 porous material Substances 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
238000000746 purification Methods 0.000 description 2
URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 2
238000005199 ultracentrifugation Methods 0.000 description 2
MJQHZNBUODTQTK-WKGBVCLCSA-N (2s,3r,4s,5r,6r)-2-[[(1s,3s,4s,5s,8r)-3-[(2s,3r,4s,5s,6r)-2-[[(1s,3r,4s,5s,8r)-3,4-dihydroxy-2,6-dioxabicyclo[3.2.1]octan-8-yl]oxy]-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-4-hydroxy-2,6-dioxabicyclo[3.2.1]octan-8-yl]oxy]-6-(hydroxymethyl)oxane-3,4,5- Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H]2OC[C@@H]1O[C@@H](O[C@@H]1[C@H]([C@H](O[C@H]3[C@H]4OC[C@@H]3O[C@@H](O)[C@H]4O)O[C@H](CO)[C@@H]1O)O)[C@H]2O MJQHZNBUODTQTK-WKGBVCLCSA-N 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
230000000259 anti-tumor effect Effects 0.000 description 1
239000008366 buffered solution Substances 0.000 description 1
229920001429 chelating resin Polymers 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
239000012847 fine chemical Substances 0.000 description 1
238000005342 ion exchange Methods 0.000 description 1
210000005170 neoplastic cell Anatomy 0.000 description 1
239000012299 nitrogen atmosphere Substances 0.000 description 1
239000002245 particle Substances 0.000 description 1
239000008194 pharmaceutical composition Substances 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
238000007127 saponification reaction Methods 0.000 description 1
238000004062 sedimentation Methods 0.000 description 1
238000000926 separation method Methods 0.000 description 1
239000002904 solvent Substances 0.000 description 1
230000006641 stabilisation Effects 0.000 description 1
238000011105 stabilization Methods 0.000 description 1
238000011282 treatment Methods 0.000 description 1
238000005406 washing Methods 0.000 description 1

Classifications

- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
- A61K9/1277—Preparation processes; Proliposomes

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Dispersion Chemistry (AREA)
Medicinal Chemistry (AREA)
Pharmacology & Pharmacy (AREA)
Epidemiology (AREA)
Life Sciences & Earth Sciences (AREA)
Animal Behavior & Ethology (AREA)
General Health & Medical Sciences (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Medicinal Preparation (AREA)

CA000312035A 1977-09-30 1978-09-25 Methode pour purifier une suspension de liposomes Expired CA1116518A (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
IT2814777		1977-09-30
IT28147A/77		1977-09-30

Publications (1)

Publication Number	Publication Date
CA1116518A true CA1116518A (fr)	1982-01-19

Family

ID=11223032

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000312035A Expired CA1116518A (fr)	1977-09-30	1978-09-25	Methode pour purifier une suspension de liposomes

Country Status (13)

Country	Link
JP (1)	JPS5459314A (fr)
AT (1)	AT368879B (fr)
AU (1)	AU528260B2 (fr)
BE (1)	BE870882A (fr)
CA (1)	CA1116518A (fr)
CH (1)	CH642540A5 (fr)
DE (1)	DE2842608C2 (fr)
DK (1)	DK154048C (fr)
FR (1)	FR2404454A1 (fr)
GB (1)	GB2004745B (fr)
NL (1)	NL7809709A (fr)
SE (1)	SE433038B (fr)
ZA (1)	ZA785510B (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IT1110989B (it) *	1979-01-19	1986-01-13	Erba Farmitalia	Forme farmaceutiche costitutite da liposomi e procedimenti relativi
JPS5775916A (en) *	1980-10-29	1982-05-12	Nippon Chemiphar Co Ltd	Coenzyme q pharmaceutical and its preparation
JPS5782310A (en) *	1980-11-11	1982-05-22	Tanabe Seiyaku Co Ltd	Production of liposome preparation
CA1270198C (fr) *	1984-08-08	1990-06-12	Marcel B Bally	Encapsulation d'agents antineoplastiques dans des liposones
US4619795A (en) *	1984-12-24	1986-10-28	Technicon Instruments Corp.	Method for preparing lipid vesicles
DE4038075C1 (en) *	1990-11-29	1992-03-19	B. Braun Melsungen Ag, 3508 Melsungen, De	Encapsulating solid or liq. lipophilic agents - comprises mixing hydration medium with phospholipid increasing temp. to above soln. phase change temp. and adding remaining medium
DE10137515A1 (de) *	2001-07-26	2003-02-13	Diagnostikforschung Inst	Verfahren zur Herstellung pharmazeutischer Zubereitungen

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE2249552A1 (de) *	1971-10-12	1973-05-30	Inchema S A	Verfahren zur inkapsulation von insbesondere wasserloeslichen verbindungen
GB1523965A (en) *	1976-03-19	1978-09-06	Ici Ltd	Pharmaceutical compositions containing steroids

1978
- 1978-09-25 NL NL7809709A patent/NL7809709A/xx not_active Application Discontinuation
- 1978-09-25 CA CA000312035A patent/CA1116518A/fr not_active Expired
- 1978-09-25 AU AU40174/78A patent/AU528260B2/en not_active Expired
- 1978-09-26 AT AT0693178A patent/AT368879B/de active
- 1978-09-26 SE SE7810109A patent/SE433038B/sv not_active IP Right Cessation
- 1978-09-26 DK DK425478A patent/DK154048C/da not_active IP Right Cessation
- 1978-09-26 FR FR7827488A patent/FR2404454A1/fr active Granted
- 1978-09-27 JP JP11809678A patent/JPS5459314A/ja active Granted
- 1978-09-28 ZA ZA00785510A patent/ZA785510B/xx unknown
- 1978-09-28 GB GB7838495A patent/GB2004745B/en not_active Expired
- 1978-09-29 CH CH1016078A patent/CH642540A5/de not_active IP Right Cessation
- 1978-09-29 DE DE2842608A patent/DE2842608C2/de not_active Expired
- 1978-09-29 BE BE190800A patent/BE870882A/fr not_active IP Right Cessation

Also Published As

Publication number	Publication date
CH642540A5 (de)	1984-04-30
GB2004745A (en)	1979-04-11
FR2404454A1 (fr)	1979-04-27
ATA693178A (de)	1982-04-15
GB2004745B (en)	1982-04-07
FR2404454B1 (fr)	1980-06-20
DE2842608A1 (de)	1979-04-12
NL7809709A (nl)	1979-04-03
SE433038B (sv)	1984-05-07
SE7810109L (sv)	1979-03-31
AU4017478A (en)	1980-04-03
ZA785510B (en)	1979-09-26
AT368879B (de)	1982-11-25
BE870882A (fr)	1979-03-29
JPS5459314A (en)	1979-05-12
JPS6134402B2 (fr)	1986-08-07
DK154048C (da)	1989-03-13
DK154048B (da)	1988-10-10
DE2842608C2 (de)	1986-02-06
DK425478A (da)	1979-03-31
AU528260B2 (en)	1983-04-21

Publication	Publication Date	Title
US4460577A (en)	1984-07-17	Pharmaceutical compositions consisting or consisting essentially of liposomes, and processes for making same
DE3785198T2 (de)	1993-07-15	Liposomes praeparat und antibiotikum.
CA1148470A (fr)	1983-06-21	Compose pharmaceutique contenant uniquement ou essentiellement des liposomes et procede de fabrication
EP0537461B1 (fr)	1996-07-24	Support chromatographique modifié
AU599456B2 (en)	1990-07-19	Phospholipid composition
EP0424698B1 (fr)	1996-12-27	Adsorbant pour éliminer du sang des biomacromolécules, en particulier les endotoxines et LDL, dans un circuit extracorporel
CA1116518A (fr)	1982-01-19	Methode pour purifier une suspension de liposomes
WO1997014964A9 (fr)	1997-07-17	Immuno-adsorbants specifiques a chaque patient pour une apherese extracorporelle et procede de fabrication correspondant
WO1997014964A2 (fr)	1997-04-24	Immuno-adsorbants specifiques a chaque patient pour une apherese extracorporelle et procede de fabrication correspondant
EP0207100B1 (fr)	1991-06-19	Procede pour modifier les dimensions effectives de pores d'une structure
DE3347512C2 (fr)	1993-06-03
Fleischer et al.	1971	Membrane associated proteins
DD262808A1 (de)	1988-12-14	Vesikulaere fuellkoerper fuer die chromatographie aus denaturierten mikroorganismen und verfahren zur herstellung
DE19740770A1 (de)	1999-03-18	Mikrofiltrationsfilterschicht sowie deren Verwendung
GB2144332A (en)	1985-03-06	Pharmaceutical preparations containing fructose-1,6-diphosphate
AU641532B2 (en)	1993-09-23	Liposome preparation and antibiotic
DE10065241A1 (de)	2001-07-26	Adorber für die extrakorporale Blutreinigung und Verfahren der Herstellung
DD251080A5 (de)	1987-11-04	Verfahren zum herstellen einer struktur mit membranen
JPH03167194A (ja)	1991-07-19	レシチンの精製方法
DE19926041A1 (de)	2000-12-21	Verfahren zur Entfernung oder Abtrennung von Viren in potentiell Viren enthaltenden Proben

Legal Events

Date	Code	Title	Description
1999-01-19	MKEX	Expiry