[go: up one dir, main page]

BR0111979A - Composição para degradar um oligossacarìdeo, método para degradar um oligossacarìdeo, célula hospedeira, métodos para realçar a degradação de um oligossacarìdeo, para fabricar uma célula hospedeira recombinante, para expressar uma endoglicanase em uma célula hospedeira, e para produzir etanol a partir de uma fonte de oligossacarìdeo, vetor, método para degradar um oligossacarìdeo, e, extrato de enzima - Google Patents

Composição para degradar um oligossacarìdeo, método para degradar um oligossacarìdeo, célula hospedeira, métodos para realçar a degradação de um oligossacarìdeo, para fabricar uma célula hospedeira recombinante, para expressar uma endoglicanase em uma célula hospedeira, e para produzir etanol a partir de uma fonte de oligossacarìdeo, vetor, método para degradar um oligossacarìdeo, e, extrato de enzima

Info

Publication number
BR0111979A
BR0111979A BR0111979-6A BR0111979A BR0111979A BR 0111979 A BR0111979 A BR 0111979A BR 0111979 A BR0111979 A BR 0111979A BR 0111979 A BR0111979 A BR 0111979A
Authority
BR
Brazil
Prior art keywords
oligosaccharide
degrading
host cell
methods
endoglycanase
Prior art date
Application number
BR0111979-6A
Other languages
English (en)
Original Assignee
Univ Florida Res Foudantion In
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida Res Foudantion In filed Critical Univ Florida Res Foudantion In
Publication of BR0111979A publication Critical patent/BR0111979A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"COMPOSIçãO PARA DEGRADAR UM OLIGOSSACARìDEO, MéTODO PARA DEGRADAR UM OLIGOSSACARìDEO, CéLULA HOSPEDEIRA, MéTODOS PARA REALçAR A DEGRADAçãO DE UM OLIGOSSACARìDEO, PARA FABRICAR UMA CéLULA HOSPEDEIRA RECOMBINANTE, PARA EXPRESSAR UMA ENDOGLICANASE EM UMA CéLULA HOSPEDEIRA, E PARA PRODUZIR ETANOL A PARTIR DE UMA FONTE DE OLIGOSSACARìDEO VETOR, MéTODO PARA DEGRADAR UM OLIGOSSACARìDEO, E, EXTRATO DE ENZIMA". A invenção fornece composições e métodos para a degradação sinergística de oligossacarídeos pelas endoglicanases. A invenção ainda fornece células hospedeiras recombinantes contendo um ou mais genes que codificam endoglicanases que são capazes da degradação sinergística de oligossacarídeos. As células hospedeiras da invenção são etanologênicas e capazes de realizar a sacarificação e fermentação simultâneas resultando na produção de etanol a partir de substratos de celulose complexos.
BR0111979-6A 2000-06-26 2001-06-19 Composição para degradar um oligossacarìdeo, método para degradar um oligossacarìdeo, célula hospedeira, métodos para realçar a degradação de um oligossacarìdeo, para fabricar uma célula hospedeira recombinante, para expressar uma endoglicanase em uma célula hospedeira, e para produzir etanol a partir de uma fonte de oligossacarìdeo, vetor, método para degradar um oligossacarìdeo, e, extrato de enzima BR0111979A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21413700P 2000-06-26 2000-06-26
US21991300P 2000-07-21 2000-07-21
PCT/US2001/019690 WO2002000858A2 (en) 2000-06-26 2001-06-19 Methods and compositions for simultaneous saccharification and fermentation

Publications (1)

Publication Number Publication Date
BR0111979A true BR0111979A (pt) 2003-07-01

Family

ID=26908719

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0111979-6A BR0111979A (pt) 2000-06-26 2001-06-19 Composição para degradar um oligossacarìdeo, método para degradar um oligossacarìdeo, célula hospedeira, métodos para realçar a degradação de um oligossacarìdeo, para fabricar uma célula hospedeira recombinante, para expressar uma endoglicanase em uma célula hospedeira, e para produzir etanol a partir de uma fonte de oligossacarìdeo, vetor, método para degradar um oligossacarìdeo, e, extrato de enzima

Country Status (12)

Country Link
US (2) US7026152B2 (pt)
EP (1) EP1299552A2 (pt)
JP (1) JP2004501636A (pt)
KR (1) KR100643817B1 (pt)
CN (1) CN1505682A (pt)
AR (1) AR036322A1 (pt)
AU (2) AU6860301A (pt)
BR (1) BR0111979A (pt)
CA (1) CA2411479A1 (pt)
MX (1) MXPA02012324A (pt)
NZ (1) NZ523192A (pt)
WO (1) WO2002000858A2 (pt)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7537826B2 (en) 1999-06-22 2009-05-26 Xyleco, Inc. Cellulosic and lignocellulosic materials and compositions and composites made therefrom
DK1581617T3 (da) * 2002-02-08 2011-05-16 Danisco Us Inc Fremgangsmåder til fremstilling af slut-produkter fra kulstof-substrater
US7504245B2 (en) * 2003-10-03 2009-03-17 Fcstone Carbon, Llc Biomass conversion to alcohol using ultrasonic energy
WO2005100582A2 (en) 2004-03-25 2005-10-27 Novozymes Inc. Methods for degrading or converting plant cell wall polysaccharides
WO2006016596A1 (ja) * 2004-08-12 2006-02-16 Oji Paper Co., Ltd. リグノセルロース材料からの繊維成分の製造およびその利用
US20150328347A1 (en) 2005-03-24 2015-11-19 Xyleco, Inc. Fibrous materials and composites
US7708214B2 (en) 2005-08-24 2010-05-04 Xyleco, Inc. Fibrous materials and composites
JP5437631B2 (ja) * 2005-07-01 2014-03-12 ザ ユニバーシティー オブ フロリダ リサーチ ファンデーション インク エタノール産生のための組み換えホスト細胞及び培地
KR100725021B1 (ko) * 2006-02-16 2007-06-07 주식회사 마크로젠 일차 대사산물의 대량 생산 방법, 대량 생산 균주 및 이의제조 방법
WO2008008793A2 (en) * 2006-07-10 2008-01-17 Dyadic International Inc. Methods and compositions for degradation of lignocellulosic material
MY157916A (en) 2006-07-21 2016-08-15 Xyleco Inc Conversion system for biomass
JP2010514448A (ja) 2006-12-28 2010-05-06 セントコア・オーソ・バイオテツク・インコーポレーテツド アシアリル化免疫グロブリンを生成するための方法およびベクター
BRPI0810375A2 (pt) * 2007-04-19 2014-10-29 Mascoma Corp Pré-tratamento termoquímico combinado e refino de biomassa lignocelulósica
KR20100029088A (ko) 2007-05-31 2010-03-15 노보자임스 인코포레이티드 셀룰로오스성 물질의 분해를 위한 조성물
CA2737544A1 (en) 2008-09-18 2010-03-25 University Of Georgia Research Foundation, Inc. Methods and compositions for degrading pectin
CA2739451A1 (en) * 2008-10-17 2010-04-22 Mascoma Corporation Production of pure lignin from lignocellulosic biomass
DK2361311T3 (en) * 2008-11-21 2017-04-24 Lallemand Hungary Liquidity Man Llc MAKE EXPRESSIVE CELLULASES FOR SIMULTANEOUS INSURANCE AND ACTION BY CELLULOSE
CN102471764B (zh) * 2009-07-03 2014-07-09 明治制果药业株式会社 包含源自两种不同的微生物的内切葡聚糖酶的纤维素酶调制物
CN107287250A (zh) * 2009-12-23 2017-10-24 丹尼斯科美国公司 提高同步糖化发酵反应效率的方法
WO2011100571A1 (en) * 2010-02-12 2011-08-18 Bp Corporation North America Inc. Bacteria capable of using cellobiose and methods of use thereof
PL3401410T3 (pl) 2010-06-26 2021-11-29 Virdia, Llc Sposoby wytwarzania mieszanek cukrów
IL206678A0 (en) 2010-06-28 2010-12-30 Hcl Cleantech Ltd A method for the production of fermentable sugars
IL207945A0 (en) 2010-09-02 2010-12-30 Robert Jansen Method for the production of carbohydrates
GB2524906B8 (en) 2011-04-07 2016-12-07 Virdia Ltd Lignocellulose conversion processes and products
US9617608B2 (en) 2011-10-10 2017-04-11 Virdia, Inc. Sugar compositions
US20130167837A1 (en) * 2011-12-30 2013-07-04 Renmatix, Inc. Compositions comprising c5 and c6 monosaccharides
GB2535435B (en) 2012-05-03 2016-12-07 Virdia Inc Methods for treating lignocellulosic materials
KR101448209B1 (ko) * 2012-09-05 2014-10-08 한국생명공학연구원 신규 당화효소 생성 미생물 및 이를 이용한 에탄올의 제조방법
CN104769116A (zh) 2012-11-09 2015-07-08 帝斯曼知识产权资产管理有限公司 酶促水解木质纤维素材料和发酵糖的方法
WO2014072390A1 (en) 2012-11-09 2014-05-15 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2014160402A1 (en) 2013-03-14 2014-10-02 Mascoma Corporation Co-conversion of carbohydrates to fermentation products in a single fermentation step
JP2016165225A (ja) 2013-07-09 2016-09-15 味の素株式会社 有用物質の製造方法
AU2015254648B2 (en) 2014-04-30 2018-11-08 Versalis S.P.A. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
WO2016054792A1 (en) 2014-10-09 2016-04-14 Cathay R&D Center Co., Ltd. Expression of recombinant tetracycline efflux pumps for the production of lysine or lysine-derived products, and methods and applications thereof
BR112017012813B1 (pt) 2014-12-19 2023-03-28 Dsm Ip Assets B.V Processo para a preparação de um produto de fermentação a partir de material lignocelulósico
EP3242871B1 (en) 2015-01-07 2019-11-06 Virdia, Inc. Methods for extracting and converting hemicellulose sugars
CN104673857A (zh) * 2015-02-13 2015-06-03 山东大学 一种葡萄糖耐受的纤维寡糖酶的应用
WO2016191503A1 (en) 2015-05-27 2016-12-01 Virdia, Inc. Integrated methods for treating lignocellulosic material

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5839517B2 (ja) * 1974-09-20 1983-08-30 カブシキガイシヤ バイオリサ−チセンタ− セルロ−スカラ アルコ−ルオセイゾウスル ホウホウ
US5162516A (en) * 1988-05-31 1992-11-10 University Of Florida Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis
US5487989A (en) * 1988-08-31 1996-01-30 Bioenergy International, L.C. Ethanol production by recombinant hosts
US5424202A (en) * 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
US5028539A (en) * 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5554520A (en) * 1988-08-31 1996-09-10 Bioenergy International, L.C. Ethanol production by recombinant hosts
US5482846A (en) * 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5000000A (en) * 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5821093A (en) * 1988-08-31 1998-10-13 University Of Florida Research Foundation, Inc. Recombinant cells that highly express chromosomally-integrated heterologous genes
WO1998045425A1 (en) 1997-04-07 1998-10-15 University Of Florida Research Foundation, Inc. DEVELOPMENT OF HIGH-ETHANOL RESISTANT $i(ESCHERICHIA COLI)
CN100335639C (zh) * 1999-05-26 2007-09-05 佛罗里达大学研究基金会 适用于同时糖化和发酵的重组宿主

Also Published As

Publication number Publication date
AU2001268603B2 (en) 2006-11-09
NZ523192A (en) 2005-03-24
US7026152B2 (en) 2006-04-11
AR036322A1 (es) 2004-09-01
MXPA02012324A (es) 2004-09-10
AU6860301A (en) 2002-01-08
CN1505682A (zh) 2004-06-16
WO2002000858A3 (en) 2002-06-13
US20020159990A1 (en) 2002-10-31
JP2004501636A (ja) 2004-01-22
EP1299552A2 (en) 2003-04-09
US20060110812A1 (en) 2006-05-25
CA2411479A1 (en) 2002-01-03
WO2002000858A2 (en) 2002-01-03
KR100643817B1 (ko) 2006-11-10
KR20030027902A (ko) 2003-04-07

Similar Documents

Publication Publication Date Title
BR0111979A (pt) Composição para degradar um oligossacarìdeo, método para degradar um oligossacarìdeo, célula hospedeira, métodos para realçar a degradação de um oligossacarìdeo, para fabricar uma célula hospedeira recombinante, para expressar uma endoglicanase em uma célula hospedeira, e para produzir etanol a partir de uma fonte de oligossacarìdeo, vetor, método para degradar um oligossacarìdeo, e, extrato de enzima
Nishitani et al. Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule.
Maeda et al. Enzymatic hydrolysis of pretreated sugar cane bagasse using Penicillium funiculosum and Trichoderma harzianum cellulases
Vanzin et al. The mur2 mutant of Arabidopsis thaliana lacks fucosylated xyloglucan because of a lesion in fucosyltransferase AtFUT1
BRPI0410520B8 (pt) produção de proteínas de fusão de glucocerebrosidase (gcd) glicosilada em cultura de plantas
TW200801195A (en) Treatment of cellulosic material and enzymes useful therein
BR0309796A (pt) Linhagens de zymomonas de fermentação de açúcar pentose e usos das mesmas, processo, método, integrante de zymomonas e biocatalisador
Pal et al. Purification and characterization of a thermostable intra-cellular β-glucosidase with transglycosylation properties from filamentous fungus Termitomyces clypeatus
BRPI0408165A (pt) processos para melhorar a atividade das enzimas de degradação de lignocelulose
BR112012008291A8 (pt) composição de enzima, célula hospedeira recombinante, e, métodos de produzir uma composição de enzima, para degradar ou converter um material celulósico, para produzir um produto de fermentação e de fermentar um material celulósico
WO2003029403A3 (en) Targeting proteins to cells expressing mannose receptors via expression in insect cells
BR0212654A (pt) Ativação e expansão de células
BRPI0814781A8 (pt) ''proteína, sequência de ácido nucleico, método para a produção de ácido itacônico, vetor, célula hospedeira, e, uso de um ácido cis-aconítico descarboxilase''
BR112012011711A2 (pt) Variantes de beta-glucosidase 1 (bgl1) com propriedades otimizadas, seu método de produção, composição, ácido nucleico, vetor de expressão, célula hospedeira, bem como método de conversão de biomassa em açúcares
WO2008006038A3 (en) Methods and compositions for butanol production
WO2008069964A3 (en) Altering regulation of maize lignin biosynthesis enzymes via rnai technology
WO2007076388A3 (en) Processes for producing a fermentation product
Villena et al. Characterization of an exocellular β-glucosidase from Debaryomyces pseudopolymorphus
BR112023008361A2 (pt) Variantes de glucoamilase e polinucleotídeos codificando as mesmas
WO2022040475A8 (en) Microbial production of cannabinoids
BRPI0910812A2 (pt) célula de fermentação de açúcar pentose
WO2008033330A3 (en) Enzyme systems for saccharification of plant cell wall polysaccharides
WO2007136971A3 (en) Process for the hydrolysis of cellulose mediated by ternary complexes of cellulose, clostridium thermocellum cells, and cellulase expressed by these cells
Gupta et al. Fermentation of pentose and hexose sugars from corncob, a low cost feedstock into ethanol
WO2023288187A3 (en) High efficency production of cannabidiolic acid

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: CONFORME ARTIGO 10O DA RESOLUCAO 124/06, CABE SER ARQUIVADO REFERENTE AO NAO RECOLHIMENTO DA 9A E 10A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2144 DE 07/02/2012.