[go: up one dir, main page]

ATE185843T1 - Nukleinsäure-sequenzanalyse durch die methode der parallelen primerextension - Google Patents

Nukleinsäure-sequenzanalyse durch die methode der parallelen primerextension

Info

Publication number
ATE185843T1
ATE185843T1 AT94921363T AT94921363T ATE185843T1 AT E185843 T1 ATE185843 T1 AT E185843T1 AT 94921363 T AT94921363 T AT 94921363T AT 94921363 T AT94921363 T AT 94921363T AT E185843 T1 ATE185843 T1 AT E185843T1
Authority
AT
Austria
Prior art keywords
polynucleotide
nucleic acid
acid sequence
sequence analysis
primer extension
Prior art date
Application number
AT94921363T
Other languages
English (en)
Inventor
C Thomas Caskey
John Shumaker
Andres Metspalu
Original Assignee
Pharmacia Biotech Ab
Baylor College Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Biotech Ab, Baylor College Medicine filed Critical Pharmacia Biotech Ab
Application granted granted Critical
Publication of ATE185843T1 publication Critical patent/ATE185843T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT94921363T 1993-06-22 1994-06-22 Nukleinsäure-sequenzanalyse durch die methode der parallelen primerextension ATE185843T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9302152A SE501439C2 (sv) 1993-06-22 1993-06-22 Sätt och anordning för analys av polynukleotidsekvenser

Publications (1)

Publication Number Publication Date
ATE185843T1 true ATE185843T1 (de) 1999-11-15

Family

ID=20390371

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94921363T ATE185843T1 (de) 1993-06-22 1994-06-22 Nukleinsäure-sequenzanalyse durch die methode der parallelen primerextension

Country Status (9)

Country Link
EP (2) EP0969103A3 (de)
JP (1) JP3722833B2 (de)
AT (1) ATE185843T1 (de)
AU (1) AU698553B2 (de)
CA (1) CA2164715A1 (de)
DE (1) DE69421277T2 (de)
ES (1) ES2141828T3 (de)
SE (1) SE501439C2 (de)
WO (1) WO1995000669A1 (de)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07203998A (ja) * 1994-01-26 1995-08-08 Hamamatsu Photonics Kk 核酸塩基配列決定方法
GB9507238D0 (en) 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5763175A (en) * 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
WO1997035033A1 (en) 1996-03-19 1997-09-25 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
US6143529A (en) * 1996-08-14 2000-11-07 Exact Laboratories, Inc. Methods for improving sensitivity and specificity of screening assays
US6096273A (en) 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US5888778A (en) * 1997-06-16 1999-03-30 Exact Laboratories, Inc. High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
US6566101B1 (en) 1997-06-16 2003-05-20 Anthony P. Shuber Primer extension methods for detecting nucleic acids
JP4789294B2 (ja) * 1997-08-22 2011-10-12 ソレクサ・インコーポレイテッド ローリングプライマーを用いたｄｎａ伸長および分析
US6322968B1 (en) 1997-11-21 2001-11-27 Orchid Biosciences, Inc. De novo or “universal” sequencing array
CA2323830A1 (en) 1998-03-16 1999-09-23 Millennium Pharmaceuticals, Inc. Methods and compositions for diagnosing and treating chromosome-18p related disorders
US6872521B1 (en) 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
US6245507B1 (en) 1998-08-18 2001-06-12 Orchid Biosciences, Inc. In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
US6150105A (en) 1998-08-20 2000-11-21 Genetic Assays, Inc. Methods of screening nucleic acids for nucleotide variations
WO2000061808A2 (en) 1999-04-09 2000-10-19 Exact Laboratories, Inc. Methods for detecting nucleic acids indicative of cancer
DE60041072D1 (de) 1999-07-26 2009-01-22 Clinical Micro Sensors Inc Em nachweis
US6528319B1 (en) * 1999-09-02 2003-03-04 Amersham Biosciences Corp Method for anchoring oligonucleotides to a substrate
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
WO2001025485A2 (en) * 1999-10-06 2001-04-12 Amersham Biosciences Corp Method for detecting mutations using arrayed primer extension
US6596483B1 (en) 1999-11-12 2003-07-22 Motorola, Inc. System and method for detecting molecules using an active pixel sensor
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
WO2001042781A2 (en) 1999-12-07 2001-06-14 Exact Sciences Corporation Supracolonic aerodigestive neoplasm detection
DE10010282B4 (de) 2000-02-25 2006-11-16 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010280B4 (de) 2000-02-25 2006-08-10 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
WO2001068913A2 (en) * 2000-03-13 2001-09-20 Genset Nucleic acid detection method and system
US6376191B1 (en) * 2000-03-22 2002-04-23 Mergen, Ltd. Microarray-based analysis of polynucleotide sequence variations
AU2001254794A1 (en) 2000-04-06 2001-10-23 Epigenomics Ag Diagnosis of diseases associated with dna adducts
EP1191036A3 (de) 2000-08-25 2002-07-03 Pfizer Products Inc. Methoden und Verbindungen zur Diagnose und Behandlung von Krankheiten, an denen Angiogenese beteiligt ist
WO2002042490A1 (en) * 2000-11-24 2002-05-30 Asper OÜ Method of optimising the sequences of synthetic nucleic acids
GB0105790D0 (en) * 2001-03-08 2001-04-25 Expresson Biosystems Ltd Detecting binding of mRNA to an oligonnucleotide array using RNA dependent nucleic acid modifying enzymes
JP2004526448A (ja) * 2001-03-30 2004-09-02 アプレラ コーポレイション 非鋳型ヌクレオチド付加を用いた核酸分析
US6653082B2 (en) 2001-05-17 2003-11-25 Baylor College Of Medicine Substrate-bound cleavage assay for nucleic acid analysis
JP4393867B2 (ja) 2001-08-14 2010-01-06 センティジェン・バイオサイエンシーズ・インコーポレイテッド 昆虫Ｏｒ８３ｂ嗅覚レセプター遺伝子の核酸およびタンパク質ならびにその使用
DE10154317B4 (de) 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
EP1340818A1 (de) 2002-02-27 2003-09-03 Epigenomics AG Verfahren und Nukleinsäuren zur Analyse von Kolonkrebszellen
DE10245145B4 (de) * 2002-09-27 2004-12-02 IPK-Institut für Pflanzengenetik und Kulturpflanzenforschung Verfahren zum Nachweis von SNPs auf polydimensionalen Microarrays
DE60328618D1 (de) 2002-10-01 2009-09-10 Epigenomics Ag Verfahren für die behandlung von proliferativen erkrankungen von brustzellen
WO2005059172A2 (en) 2003-12-11 2005-06-30 Epigenomics Ag Method and nucleic acids for the improved treatment of breast cell proliferative disorders
US9045796B2 (en) 2003-06-20 2015-06-02 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
EP2354250A1 (de) 2003-06-23 2011-08-10 Epigenomics AG Verfahren und Nukleinsäuren zur Analyse von Störungen der Proliferation kolorektaler Zellen
US20080064029A1 (en) 2003-06-23 2008-03-13 Epigenomics Ag Methods and Nucleic Acids for Analyses of Colorectal Cell Proliferative Disorders
EP1636381B1 (de) 2003-06-23 2011-01-05 Epigenomics AG Verfahren und nukleinsäuren zur analyse von störungen der proliferation von kolonzellen
EP1689889B1 (de) 2003-12-01 2017-08-30 Epigenomics AG Verfahren und nukleinsäuren zur analyse der mit der entwicklung von prostatazellproliferationsstörungen assoziierten genexpression
EP1778865A1 (de) 2004-06-23 2007-05-02 Epigenomics AG Verfahren und nukleinsäuren zum nachweis der metastasierung proliferativer kolonzellerkrankungen
EP1769086A2 (de) 2004-07-18 2007-04-04 Epigenomics AG Epigenetische verfahren und nukleinsäuren zum nachweis von proliferativen erkrankungen von brustzellen
EP1784508B1 (de) 2004-08-09 2012-10-03 Generation Biotech, LLC Verfahren zur isolierung und amplifikation von nukleinsäuren
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US20090197250A1 (en) 2004-12-02 2009-08-06 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with the prognosis of prostate cell proliferative disorders
CN114921544A (zh) 2005-04-15 2022-08-19 Epi基因组股份公司 分析细胞增殖性病症的方法和核酸
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
WO2006119434A2 (en) 2005-05-02 2006-11-09 University Of Southern California DNA METHYLATION MARKERS ASSOCIATED WITH THE CpG ISLAND METHYLATOR PHENOTYPE (CIMP) IN HUMAN COLORECTAL CANCER
WO2007039234A2 (en) 2005-09-29 2007-04-12 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with tissue classification
WO2007057231A1 (en) 2005-11-17 2007-05-24 Epigenomics Ag Method for the determination of the dna methylation level of a cpg position in identical cells within a tissue sample
CA2649777C (en) 2006-04-17 2018-08-14 Epigenomics Ag Methods and nucleic acids for the detection of colorectal cell proliferative disorders
US20100003189A1 (en) 2006-07-14 2010-01-07 The Regents Of The University Of California Cancer biomarkers and methods of use thereof
ES2546182T3 (es) 2006-07-21 2015-09-21 Epigenomics Ag Métodos y kits para análisis de metilación en trastornos proliferativos celulares colorrectales
US20100203514A1 (en) 2006-11-24 2010-08-12 Sledziewski Andrew Z Methods and nucleic acids for the analysis of gene expression associated with the development of prostate cell proliferative disorders
AU2008207110B2 (en) 2007-01-19 2013-10-17 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
CN101743326A (zh) * 2007-05-14 2010-06-16 因赛特遗传学公司 筛选核酸中的单核苷酸变异的方法
US7635566B2 (en) 2007-06-29 2009-12-22 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
CA2708163A1 (en) 2007-12-11 2009-06-18 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
ES2669439T3 (es) 2009-06-26 2018-05-25 Epigenomics Ag Métodos y ácidos nucleicos para el análisis del carcinoma de vejiga
US20110104695A1 (en) 2009-11-05 2011-05-05 Epigenomics Ag Methods of predicting therapeutic efficacy of cancer therapy
US20120003639A1 (en) 2010-04-27 2012-01-05 Prelude, Inc. Cancer biomarkers and methods of use thereof
CN108048573A (zh) 2011-07-08 2018-05-18 表观基因组股份有限公司 用于确定癌症对象之预后的方法和核酸
WO2014160233A1 (en) 2013-03-13 2014-10-02 Abbott Molecular Inc. Systems and methods for isolating nucleic acids
JP2016512437A (ja) 2013-03-14 2016-04-28 アボツト・モレキユラー・インコーポレイテツド 多重メチル化特異的増幅システムおよび方法
CN105143465A (zh) 2013-03-14 2015-12-09 梅奥医学教育和研究基金会 检测赘生物
WO2015124921A1 (en) 2014-02-19 2015-08-27 The University Court Of The University Of Edinburgh Methods and uses for determining the presence of inflammatory bowel disease
US11078539B2 (en) 2014-03-31 2021-08-03 Mayo Foundation For Medical Education And Research Detecting colorectal neoplasm
US10184154B2 (en) 2014-09-26 2019-01-22 Mayo Foundation For Medical Education And Research Detecting cholangiocarcinoma
WO2016090323A1 (en) 2014-12-05 2016-06-09 Prelude, Inc. Dcis recurrence and invasive breast cancer
CN107003314B (zh) 2014-12-12 2021-10-15 精密科学公司 用于进行甲基化检测测定的组合物和方法
EP3274440A4 (de) 2015-03-27 2019-03-06 Exact Sciences Corporation Nachweis von erkrankungen der speiseröhre
WO2017075061A1 (en) 2015-10-30 2017-05-04 Exact Sciences Corporation Multiplex amplification detection assay and isolation and detection of dna from plasma
AU2017260630B2 (en) 2016-05-05 2023-07-13 Exact Sciences Corporation Detection of lung neoplasia by analysis of methylated DNA
ES2902174T3 (es) 2016-07-19 2022-03-25 Exact Sciences Dev Co Llc ADN de control metilado
CN116064795A (zh) 2016-09-02 2023-05-05 梅约医学教育与研究基金会 确定差异甲基化区域的甲基化状态的方法和试剂盒
JP7289264B2 (ja) 2017-01-27 2023-06-09 エグザクト サイエンシーズ コーポレーション メチル化ｄｎａの分析による結腸腫瘍の検出
JP7277460B2 (ja) 2017-11-30 2023-05-19 マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ 乳癌の検出
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
AU2019339508A1 (en) 2018-09-14 2021-04-15 Prelude Corporation Method of selection for treatment of subjects at risk of invasive breast cancer
WO2020099938A2 (en) 2018-11-16 2020-05-22 Oslo Universitetssykehus Hf Methods and compositions for characterizing bladder cancer
CN114729399A (zh) 2019-10-31 2022-07-08 梅约医学教育与研究基金会 检测卵巢癌
AU2021329899A1 (en) 2020-08-19 2023-03-09 Exact Sciences Corporation Detecting non-hodgkin lymphoma
AU2022213409A1 (en) 2021-01-29 2023-08-17 Exact Sciences Corporation Detecting the presence or absence of multiple types of cancer

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5202231A (en) * 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
GB8810400D0 (en) * 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
DE69132843T2 (de) * 1990-12-06 2002-09-12 Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) Identifizierung von Nukleinsäuren in Proben
US6004744A (en) * 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer

Also Published As

Publication number Publication date
AU7212194A (en) 1995-01-17
EP0705349B1 (de) 1999-10-20
AU698553B2 (en) 1998-10-29
EP0969103A3 (de) 2004-01-02
EP0969103A2 (de) 2000-01-05
DE69421277T2 (de) 2000-05-31
ES2141828T3 (es) 2000-04-01
WO1995000669A1 (en) 1995-01-05
SE501439C2 (sv) 1995-02-13
DE69421277D1 (de) 1999-11-25
JP3722833B2 (ja) 2005-11-30
EP0705349A1 (de) 1996-04-10
CA2164715A1 (en) 1995-01-05
JPH09501312A (ja) 1997-02-10
SE9302152D0 (sv) 1993-06-22
SE9302152L (sv) 1994-12-23

Similar Documents

Publication Publication Date Title
DE69421277D1 (de) NUKLEINSäURE-SEQUENZANALYSE DURCH DIE METHODE DER PARALLELEN PRIMEREXTENSION
RU2400538C2 (ru) Олигонуклеотид с двойственной специфичностью и способы, в которых он используется
EP0777747B1 (de) Nukleotid-sequenzierungsmethode
US20020187477A1 (en) Method for detecting single nucleotide polymorphisms (SNPs) and point mutations
CA2497740A1 (en) Multiplexed analysis of polymorphic loci by probe elongation-mediated detection
EP1613773B1 (de) Methode zur charakterisierung von nukleinsäuren
ATE328115T1 (de) Multiplex genotypisierung unter verwendung von fluoreszenzmarkierten hybridisierungssonden
CA2239287A1 (en) A cascade nucleic acid amplification reaction
IL135852A0 (en) A method for detecting dna base mismatch
DE69428252D1 (de) Verfahren zur Verbesserung der Nukleinsäureamplifizierung
ATE164883T1 (de) Verfahren zur vorbereitung von doppel strangiger rna und seine anwendungen
CA2544202A1 (en) Multiplexed nucleic acid analysis by fragmentation of double-stranded dna
WO2003020968A3 (de) Verfahren zur analyse von nukleinsäurekettensequenzen und der genexpression
NZ506333A (en) An assay for detecting and quantifying the presence of particular nucleic acid (DNA or RNA) sequences
PT92420A (pt) Metodo e combinacao de reagentes para a determinacao de sequencias de nucleotidos
CA2490864A1 (en) Methods of detecting sequence differences
DE60113945D1 (de) Verfahren zur Analyse von Wiederkehrenden PCR-Podukten, das auf der Analyse von DNS-Schmelzkurven basiert
ATE361377T1 (de) Leistungstarke verknüpfung von nukleinsäuresegmenten
EP0855447A3 (de) Verfahren zur Bestimmung von Nukleinsäuresequenzen
ATE272722T1 (de) Verfahren zur kontrollierbaren durchführung komplexer pcr-amplifikationen
EP0509089A1 (de) Verbindungen und methoden zur analyse von genomischen variationen
DE50213026D1 (de) Tifikation in einer probe
GB2295228A (en) Selective amplification of DNA having a plurality of sites for a specific endonuclease
ATE483031T1 (de) Oligonukleotidprimer für das stn gen zur detektion von salmonella arten und entsprechende nachweismethoden
WO2002079496A3 (en) Method and sequences for determinate nucleic acid hybridization

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EEFA Change of the company name
REN Ceased due to non-payment of the annual fee