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AR114014A1 - BASE EDITING SYSTEM AND METHOD BASED ON PROTEIN CPF1 - Google Patents

BASE EDITING SYSTEM AND METHOD BASED ON PROTEIN CPF1

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Publication number
AR114014A1
AR114014A1 ARP180103817A ARP180103817A AR114014A1 AR 114014 A1 AR114014 A1 AR 114014A1 AR P180103817 A ARP180103817 A AR P180103817A AR P180103817 A ARP180103817 A AR P180103817A AR 114014 A1 AR114014 A1 AR 114014A1
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AR
Argentina
Prior art keywords
base editing
fusion protein
guide rna
nucleotide sequence
sequence encoding
Prior art date
Application number
ARP180103817A
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Spanish (es)
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Inst Genetics & Developmental Biology Cas
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Publication of AR114014A1 publication Critical patent/AR114014A1/en

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/14—Hydrolases (3)
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    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04001—Cytosine deaminase (3.5.4.1)
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente se relaciona con el campo de la ingeniería genética. En particular, la presente se relaciona con un sistema y método para la edición de bases basado en la proteína CPF1. Más particularmente, la presente se relaciona con un sistema y método para la edición eficaz de bases de una secuencia objetivo en el genoma de un organismo (por ejemplo, una planta) a través de una proteína de fusión CpfI-desaminasa dirigida por ARN guía, y el organismo genéticamente modificado (por ejemplo, plantas) producido por el método y su progenie. Reivindicación 1: Un sistema para la edición de bases de una secuencia objetivo en el genoma de un organismo, que comprende por lo menos uno de los siguientes puntos i) a v): i) una proteína de fusión para la edición de bases, y un ARN guía; ii) una construcción de expresión que comprende una secuencia de nucleótidos que codifica una proteína de fusión para la edición de bases, y un ARN guía; iii) una proteína de fusión para la edición de bases, y una construcción de expresión que comprende una secuencia de nucleótidos que codifican un ARN guía; iv) una construcción de expresión que comprende una secuencia de nucleótidos que codifica una proteína de fusión para la edición de bases, y una construcción de expresión que comprende una secuencia de nucleótidos que codifica un ARN guía; v) una construcción de expresión que comprende una secuencia de nucleótidos que codifica una proteína de fusión para la edición de bases y una secuencia de nucleótidos que codifica un ARN guía; en donde la proteína de fusión para la edición de bases comprende una Cpf1 que carece de actividad de escisión de ADN y una desamina, el ARN guía es capaz de dirigir la proteína de fusión para la edición de bases a una secuencia objetivo en el genoma, que resulta en una o más sustituciones de C a T o de A a G en la secuencia objetivo.This is related to the field of genetic engineering. In particular, this relates to a system and method for base editing based on the CPF1 protein. More particularly, this relates to a system and method for efficient base editing of a target sequence in the genome of an organism (eg, a plant) through a guide RNA-directed CpfI-deaminase fusion protein, and the genetically modified organism (eg, plants) produced by the method and its progeny. Claim 1: A system for the base editing of a target sequence in the genome of an organism, comprising at least one of the following items i) to v): i) a fusion protein for base editing, and a Guide RNA; ii) an expression construct comprising a nucleotide sequence encoding a fusion protein for base editing, and a guide RNA; iii) a fusion protein for base editing, and an expression construct comprising a nucleotide sequence encoding a guide RNA; iv) an expression construct comprising a nucleotide sequence encoding a fusion protein for base editing, and an expression construct comprising a nucleotide sequence encoding a guide RNA; v) an expression construct comprising a nucleotide sequence encoding a fusion protein for base editing and a nucleotide sequence encoding a guide RNA; wherein the base editing fusion protein comprises a Cpf1 lacking DNA cleavage activity and a deamine, the guide RNA is capable of directing the base editing fusion protein to a target sequence in the genome, resulting in one or more C to T or A to G substitutions in the target sequence.

ARP180103817A 2017-12-22 2018-12-21 BASE EDITING SYSTEM AND METHOD BASED ON PROTEIN CPF1 AR114014A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711403490 2017-12-22

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AR114014A1 true AR114014A1 (en) 2020-07-08

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Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210023831A (en) 2018-05-11 2021-03-04 빔 테라퓨틱스, 인크. How to Replace Pathogenic Amino Acids Using a Programmable Base Editor System
SG11202106977PA (en) * 2018-12-27 2021-07-29 Lifeedit Therapeutics Inc Polypeptides useful for gene editing and methods of use
WO2020168133A1 (en) * 2019-02-13 2020-08-20 Beam Therapeutics Inc. Compositions and methods for treating hemoglobinopathies
CN114375335A (en) * 2019-07-19 2022-04-19 成对植物服务股份有限公司 Optimized protein linkers and methods of use
WO2021032155A1 (en) * 2019-08-20 2021-02-25 中国科学院遗传与发育生物学研究所 Base editing system and use method therefor
CN110551752B (en) * 2019-08-30 2023-03-14 北京市农林科学院 xCas9n-epBE base editing system and application thereof in genome base replacement
US20230075877A1 (en) * 2019-09-09 2023-03-09 Beam Therapeutics Inc. Novel nucleobase editors and methods of using same
US20230257761A1 (en) 2019-09-12 2023-08-17 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Herbicide resistant plant
WO2021056302A1 (en) * 2019-09-26 2021-04-01 Syngenta Crop Protection Ag Methods and compositions for dna base editing
US11866745B2 (en) * 2019-10-17 2024-01-09 Pairwise Plants Services, Inc. Variants of CAS12A nucleases and methods of making and use thereof
JP2023501223A (en) * 2019-10-30 2023-01-18 ペアーワイズ プランツ サービシズ， インコーポレイテッド Type V CRISPR-CAS base editor and method of use
CN111019967A (en) * 2019-11-27 2020-04-17 南京农业大学 Application of GmU3-19g-1 and GmU6-16g-1 promoters in soybean polygene editing system
CN110964741B (en) * 2019-12-20 2022-03-01 北京市农林科学院 A nuclear localization signal FNB and its application in improving base editing efficiency
CN111518794B (en) * 2020-04-13 2023-05-16 中山大学 Preparation and application of inducible mutant protein based on activation-inducible cytidine deaminase
CN112851776B (en) * 2020-04-20 2022-08-30 中国科学院天津工业生物技术研究所 Gene site-directed mutagenesis method and stress resistance breeding application thereof
EP4185699A1 (en) 2020-07-21 2023-05-31 Pairwise Plants Services, Inc. Optimized protein linkers and methods of use
CN114317596B (en) * 2020-09-30 2024-01-16 北京市农林科学院 A method for mutating A to G in target sequence of plant genome
CN114317518B (en) * 2020-09-30 2024-01-12 北京市农林科学院 Application of SpRYn-CBE base editing system in base replacement in plant genomes
CN114317589B (en) * 2020-09-30 2024-01-16 北京市农林科学院 Application of SpRYn-ABE base editing system in plant genome base substitution
CN112430622A (en) * 2020-10-26 2021-03-02 扬州大学 FokI and dCpf1 fusion protein expression vector and site-directed gene editing method mediated by same
CN113005141A (en) * 2021-01-05 2021-06-22 温州医科大学 Gene editing tool composed of high-activity mutant, preparation method and method for repairing congenital retinoschisis disease pathogenic gene
CA3216308A1 (en) * 2021-04-21 2022-10-27 Zhejiang University Negative-strand rna viral vector and plant genome editing method without transformation
CN115704015A (en) * 2021-08-12 2023-02-17 清华大学 Targeted mutagenesis system based on adenine and cytosine dual base editor
CN114045302A (en) * 2021-11-12 2022-02-15 三亚中国农业科学院国家南繁研究院 Single-base editing vector and construction and application thereof
CN114835818B (en) * 2022-03-17 2024-03-22 江南大学 Gene editing fusion protein, adenine base editor constructed by same and application thereof
EP4499819A1 (en) * 2022-03-30 2025-02-05 Basf Agricultural Solutions Seed Us Llc Optimized base editors
WO2023207607A1 (en) * 2022-04-29 2023-11-02 北京大学 Deaminase mutant, composition, and method for modifying mitochondrial dna
CN114686456B (en) * 2022-05-10 2023-02-17 中山大学 Base editing system based on bimolecular deaminase complementation and application thereof
CN115820691B (en) * 2022-07-25 2023-08-22 安徽农业大学 LbCPf1 variant-based rice base editing system and application
CN116376948B (en) * 2022-07-25 2023-12-15 广州医科大学 Plasmid vector and preparation method of MS2 phage similar particles for displaying exogenous proteins
CN116286734B (en) * 2022-11-29 2024-04-02 武汉大学 Mutants of wild-type LbCas12a protein and their uses for SNP detection
CN116751799B (en) * 2023-06-14 2024-01-26 江南大学 Multi-site double-base editor and application thereof
CN117965505A (en) * 2023-06-28 2024-05-03 微光基因(苏州)有限公司 Engineered adenosine deaminase and base editors

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3374494A4 (en) * 2015-11-11 2019-05-01 Coda Biotherapeutics, Inc. Crispr compositions and methods of using the same for gene therapy
EP3405570A1 (en) * 2016-01-22 2018-11-28 The Broad Institute, Inc. Crystal structure of crispr cpf1
US20210155911A1 (en) * 2016-04-19 2021-05-27 The Broad Institute, Inc. Novel crispr enzymes and systems
WO2017189308A1 (en) * 2016-04-19 2017-11-02 The Broad Institute Inc. Novel crispr enzymes and systems
AU2017253107B2 (en) * 2016-04-19 2023-07-20 Massachusetts Institute Of Technology CPF1 complexes with reduced indel activity
GB2568182A (en) * 2016-08-03 2019-05-08 Harvard College Adenosine nucleobase editors and uses thereof

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CN109957569B (en) 2022-10-25
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