AR082133A1 - Proteina transportadora de glucosinolato y sus usos - Google Patents
Proteina transportadora de glucosinolato y sus usosInfo
- Publication number
- AR082133A1 AR082133A1 ARP110102450A ARP110102450A AR082133A1 AR 082133 A1 AR082133 A1 AR 082133A1 AR P110102450 A ARP110102450 A AR P110102450A AR P110102450 A ARP110102450 A AR P110102450A AR 082133 A1 AR082133 A1 AR 082133A1
- Authority
- AR
- Argentina
- Prior art keywords
- seq
- gtr
- plant
- protein
- mutant
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 102000004169 proteins and genes Human genes 0.000 title abstract 6
- 241000196324 Embryophyta Species 0.000 abstract 11
- 125000004383 glucosinolate group Chemical group 0.000 abstract 8
- 108700028369 Alleles Proteins 0.000 abstract 6
- 108020005038 Terminator Codon Proteins 0.000 abstract 6
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 5
- 238000000034 method Methods 0.000 abstract 5
- 108020004707 nucleic acids Proteins 0.000 abstract 5
- 102000039446 nucleic acids Human genes 0.000 abstract 5
- 150000007523 nucleic acids Chemical class 0.000 abstract 5
- 241000218980 Brassicales Species 0.000 abstract 2
- 238000006467 substitution reaction Methods 0.000 abstract 2
- 108010078791 Carrier Proteins Proteins 0.000 abstract 1
- 235000013312 flour Nutrition 0.000 abstract 1
- 230000005714 functional activity Effects 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nutrition Science (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Virology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
- Physiology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Métodos y medios para alterar el contenido de glucosinolato (GSL) en las plantas, particularmente. Métodos para reducir el contenido de GSL en las semillas de las plantas y en la harina derivada de ellas. Métodos para incrementar el contenido de GSL en el tejido verde de las plantas del orden Brassicales.Reivindicación 1: Un método para modificar el contenido de glucosinolato (GSL) en una planta del orden Brassicales o en una parte de ésta, caracterizado porque comprende modificar la actividad funcional de al menos una proteína transportadora de glucosinolato (GTR) que comprende una secuencia de aminoácidos que tiene una identidad de al menos 33% con SEQ ID Nº 2 o con SEQ ID Nº 142 en células de dicha planta o de dicha parte de planta. Reivindicación 19: Una planta o una parte de una planta caracterizadas porque pueden obtenerse con el método de cualquiera de las reivindicaciones 1 a 17. Reivindicación 20: Semillas caracterizadas porque provienen de la planta de la reivindicación 19. Reivindicación 21: Uso de una secuencia de ácido nucleico que codifica una proteína GTR, caracterizado porque es en la obtención de una planta o una parte de planta con un contenido modificado de GSL. Reivindicación 27: Una proteína aislada caracterizada porque comprende una secuencia de aminoácidos de SEQ ID Nº 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 120, 122,124, 126, 128, 130, 142, 144, 146, 148 ó 150. Reivindicación 28: Un alelo de GTR mutante caracterizado porque codifica una proteína GTR que presenta una secuencia de aminoácidos que tiene una identidad de al menos 80% con SEQ ID Nº 2 o con SEQ ID Nº 142 y presenta cualquiera de las siguientes sustituciones: a) la S en la posición 22 por A (la posición 52 de SEQ ID Nº 142), b) la S en la posición 22 por D (la posición 52 de SEQ ID Nº 142), c) la T en la posición 105 por A (la posición 135 de SEQ ID Nº 142), d) la T en la posición 105 por D (la posición 135 de SEQ ID Nº 142), e) la S en la posición 605 por A (la posición 635 de SEQ ID Nº 142), f) la S en la posición 605 por D (la posición 635 de SEQ ID Nº 142); o una proteína GTR que tiene una identidad de al menos 80% con la secuencia de aminoácidos de SEQ ID Nº 4 y presenta las siguientes sustituciones: g) la T en la posición 58 por A, h) la T en la posición 58 por D, i) la T en la posición 117 por A, j) la T en la posición 117 por D, k) la T en la posición 323 por A, l) la T en la posición 323 por D; o un alelo de GTR mutante que tiene una identidad de secuencia de al menos 80% con un ácido nucleico de SEQ ID Nº 25 y comprende un codón de terminación en la posición 1241 a 1243; o un alelo de GTR mutante que tiene una identidad de secuencia de al menos 80% con un ácido nucleico de SEQ ID Nº 31 y a) comprende un codón de terminación en las posiciones 929 a 931, o b) comprende un codón de terminación en las posiciones 1145 a 1147; o un alelo de GTR mutante que tiene una identidad de secuencia de al menos 80% con un ácido nucleico de SEQ ID Nº 27 y a) comprende un codón de terminación en las posiciones 870 a 872, o b) comprende un codón de terminación en las posiciones 1380 a 1382; o un alelo de GTR mutante que tiene una identidad de secuencia de al menos 80% con un ácido nucleico de SEQ ID Nº 33 y comprende un codón de terminación en las posiciones 780 a 782; o un alelo de GTR mutante que codifica una proteína GTR que tiene una identidad de al menos 80% con la secuencia de aminoácidos de SEQ ID Nº 66 y presenta una mutación en cualquiera de las siguientes posiciones: a) Gly 126, b) Gly 145, c) Glu 192, d) Trp 229, e) Ser 359.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36239010P | 2010-07-08 | 2010-07-08 | |
| EP10075299 | 2010-07-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR082133A1 true AR082133A1 (es) | 2012-11-14 |
Family
ID=44785797
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP110102450A AR082133A1 (es) | 2010-07-08 | 2011-07-08 | Proteina transportadora de glucosinolato y sus usos |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20140137294A1 (es) |
| EP (1) | EP2590996B1 (es) |
| JP (1) | JP2013531502A (es) |
| CN (1) | CN103124738B (es) |
| AR (1) | AR082133A1 (es) |
| AU (1) | AU2011276127B2 (es) |
| CL (1) | CL2013000032A1 (es) |
| EA (1) | EA201390077A1 (es) |
| WO (1) | WO2012004013A2 (es) |
| ZA (1) | ZA201300211B (es) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20210084936A1 (en) | 2018-01-19 | 2021-03-25 | Covercress Inc. | Low glucosinolate pennycress meal and methods of making |
| US11178834B2 (en) * | 2018-05-29 | 2021-11-23 | Nuseed Global Innovation Ltd. | Brassica carinata varieties producing seed with reduced glucosinolate content |
| AU2019329686A1 (en) | 2018-08-27 | 2020-12-03 | Regeneron Pharmaceuticals, Inc. | Use of raman spectroscopy in downstream purification |
| CN110033825B (zh) * | 2019-04-26 | 2021-08-13 | 扬州大学 | 一种箭筈豌豆种质资源筛选方法 |
| CN110438146A (zh) * | 2019-06-25 | 2019-11-12 | 武汉市农业科学院 | 一种获得种子低硫苷含量的油菜品种的方法 |
| CN112877341B (zh) * | 2021-04-02 | 2023-03-14 | 中国农业科学院油料作物研究所 | 硫苷转运相关的甘蓝型油菜BnaA09.GTR2基因及其应用 |
| CN113061615B (zh) * | 2021-04-02 | 2023-04-07 | 中国农业科学院油料作物研究所 | 硫苷转运相关的甘蓝型油菜BnaA06.GTR2基因及其应用 |
| CN114836559B (zh) * | 2022-03-28 | 2023-09-01 | 浙江省农业科学院 | 与西兰花中2-羟基-3-丁烯基芥子油苷含量相关的snp标记及引物和应用 |
| CA3259474A1 (en) | 2022-06-17 | 2023-12-21 | National Institute Of Plant Genome Research | RECOMMENDING EXPRESSION CASSETTES FOR MODIFYING GLUCOSINOLATE CONTENT IN PLANTS |
| CA3269000A1 (en) | 2022-11-16 | 2024-05-23 | University Of Copenhagen | PLANTS WITH LOW GLUCOSINOLATES IN THE SEEDS |
Family Cites Families (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4407956A (en) | 1981-03-13 | 1983-10-04 | The Regents Of The University Of California | Cloned cauliflower mosaic virus DNA as a plant vehicle |
| CA1192510A (en) | 1981-05-27 | 1985-08-27 | Lawrence E. Pelcher | Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom |
| NL8200523A (nl) | 1982-02-11 | 1983-09-01 | Univ Leiden | Werkwijze voor het in vitro transformeren van planteprotoplasten met plasmide-dna. |
| US4536475A (en) | 1982-10-05 | 1985-08-20 | Phytogen | Plant vector |
| EP0290799B9 (en) | 1983-01-13 | 2004-09-01 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Transgenic dicotyledonous plant cells and plants |
| US5352605A (en) | 1983-01-17 | 1994-10-04 | Monsanto Company | Chimeric genes for transforming plant cells using viral promoters |
| DE3484215D1 (de) | 1983-01-17 | 1991-04-11 | Monsanto Co | Chimaerische gene geeignet zur expression in pflanzenzellen. |
| DE3464841D1 (en) | 1983-01-27 | 1987-08-27 | Gen Foods Corp | Water-agglomeration method for depeptide sweetened products |
| NL8300699A (nl) | 1983-02-24 | 1984-09-17 | Univ Leiden | Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten. |
| US5504200A (en) | 1983-04-15 | 1996-04-02 | Mycogen Plant Science, Inc. | Plant gene expression |
| WO1985001856A1 (en) | 1983-11-03 | 1985-05-09 | Johannes Martenis Jacob De Wet | Method for the transfer of exogenous genes in plants using pollen as a vector |
| US5420034A (en) | 1986-07-31 | 1995-05-30 | Calgene, Inc. | Seed-specific transcriptional regulation |
| FI864720A7 (fi) | 1985-11-22 | 1987-05-23 | Ciba Geigy Ag | Suora geeninsiirto plastideihin ja mitokondrioihin. |
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| EP0265556A1 (en) | 1986-10-31 | 1988-05-04 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Stable binary agrobacterium vectors and their use |
| IL84459A (en) | 1986-12-05 | 1993-07-08 | Agracetus | Apparatus and method for the injection of carrier particles carrying genetic material into living cells |
| GB8810120D0 (en) | 1988-04-28 | 1988-06-02 | Plant Genetic Systems Nv | Transgenic nuclear male sterile plants |
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| US5846717A (en) | 1996-01-24 | 1998-12-08 | Third Wave Technologies, Inc. | Detection of nucleic acid sequences by invader-directed cleavage |
| US5527695A (en) | 1993-01-29 | 1996-06-18 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
| NZ278490A (en) | 1993-12-09 | 1998-03-25 | Univ Jefferson | Chimeric polynucleotide with both ribo- and deoxyribonucleotides in one strand and deoxyribonucleotides in a second strand |
| JPH10505233A (ja) | 1994-08-30 | 1998-05-26 | コモンウェルス サイエンティフック アンド インダストリアル リサーチ オーガニゼーション | シルコウイルス由来の植物転写調節因子 |
| US5780296A (en) | 1995-01-17 | 1998-07-14 | Thomas Jefferson University | Compositions and methods to promote homologous recombination in eukaryotic cells and organisms |
| FR2734842B1 (fr) | 1995-06-02 | 1998-02-27 | Rhone Poulenc Agrochimie | Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un gene de l'hydroxy-phenyl pyruvate dioxygenase, tolerantes a certains herbicides |
| FR2736926B1 (fr) | 1995-07-19 | 1997-08-22 | Rhone Poulenc Agrochimie | 5-enol pyruvylshikimate-3-phosphate synthase mutee, gene codant pour cette proteine et plantes transformees contenant ce gene |
| ATE331034T1 (de) | 1995-10-06 | 2006-07-15 | Bayer Bioscience Nv | Samenstrenung resistenz |
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| CA2325344C (en) | 1998-04-08 | 2017-12-05 | Commonwealth Scientific And Industrial Research Organisation | Methods and means for obtaining modified phenotypes |
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| WO2002010210A2 (en) * | 2001-08-28 | 2002-02-07 | Bayer Cropscience Ag | Polypeptides for identifying herbicidally active compounds |
| CA2470426A1 (en) | 2001-12-18 | 2003-06-26 | Bayer Bioscience N.V. | Improved methods and means for delivering inhibitory rna to plants and applications thereof |
| AU2003209814B2 (en) | 2002-03-14 | 2008-12-04 | Commonwealth Scientific & Industrial Research Organisation | Modified gene-silencing RNA and uses thereof |
| US20060150283A1 (en) * | 2004-02-13 | 2006-07-06 | Nickolai Alexandrov | Sequence-determined DNA fragments and corresponding polypeptides encoded thereby |
| WO2005098004A2 (en) | 2004-04-07 | 2005-10-20 | Bayer Bioscience N.V. | Inducible boost of integrated satellite rna viruses |
| JP2007295918A (ja) * | 2006-04-07 | 2007-11-15 | Institute Of Physical & Chemical Research | 硫黄同化系制御因子を利用した植物の作出方法 |
| CA2661325A1 (en) * | 2006-08-22 | 2008-02-28 | University Of Copenhagen | Flavin monooxygenases and transcription factors involved in glucosinolate biosynthesis |
| JP2009284799A (ja) * | 2008-05-28 | 2009-12-10 | Institute Of Physical & Chemical Research | メチオニン由来グルコシノレート生合成抑制遺伝子を利用した植物の作出方法 |
| JP2010022210A (ja) * | 2008-07-15 | 2010-02-04 | Institute Of Physical & Chemical Research | メチオニン由来グルコシノレート及びメチオニンの蓄積量が制御された植物及びその作出方法 |
-
2011
- 2011-07-06 US US13/808,024 patent/US20140137294A1/en not_active Abandoned
- 2011-07-06 JP JP2013517110A patent/JP2013531502A/ja active Pending
- 2011-07-06 WO PCT/EP2011/004565 patent/WO2012004013A2/en not_active Ceased
- 2011-07-06 CN CN201180040120.9A patent/CN103124738B/zh active Active
- 2011-07-06 EA EA201390077A patent/EA201390077A1/ru unknown
- 2011-07-06 EP EP11767621.3A patent/EP2590996B1/en active Active
- 2011-07-06 AU AU2011276127A patent/AU2011276127B2/en active Active
- 2011-07-08 AR ARP110102450A patent/AR082133A1/es unknown
-
2013
- 2013-01-04 CL CL2013000032A patent/CL2013000032A1/es unknown
- 2013-01-09 ZA ZA2013/00211A patent/ZA201300211B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ZA201300211B (en) | 2014-03-26 |
| EP2590996B1 (en) | 2016-05-25 |
| EP2590996A2 (en) | 2013-05-15 |
| CA2804487A1 (en) | 2012-01-12 |
| CL2013000032A1 (es) | 2014-02-07 |
| WO2012004013A2 (en) | 2012-01-12 |
| US20140137294A1 (en) | 2014-05-15 |
| CN103124738A (zh) | 2013-05-29 |
| AU2011276127A1 (en) | 2013-02-14 |
| AU2011276127B2 (en) | 2015-03-19 |
| EA201390077A1 (ru) | 2013-06-28 |
| WO2012004013A3 (en) | 2012-04-26 |
| JP2013531502A (ja) | 2013-08-08 |
| CN103124738B (zh) | 2016-08-17 |
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