[go: up one dir, main page]
Skip to main content

Advertisement

SpringerLink
Log in

Biodegradation of Cypermethrin by a newly isolated actinomycetes HU-S-01 from wastewater sludge

  • Published:
International Journal of Environmental Science & Technology Aims and scope Submit manuscript

Abstract

A new cypermethrin degrading strain was isolated from wastewater sludge using enrichment technique. On the basis of morphological, physiological, biochemical characteristics tests and 16S rDNA sequence analysis, the strain was determined to be a Streptomyces species, probably a strain of Streptomyces parvulus, so it was designated as Streptomyces sp. HU-S-01. The strain Streptomyces sp. HU-S-01 is aerobic and optimum growth temperature for the strain was found to be 26–28 °C with initial pH range 6.0-9.0 and pH 7.5 was found to be the optimum. This strain can also completely degrade 3-phenoxybenzoic acid within 96 h at the concentration of 50 mg/L. The kinetic constants Vmax, Km, Kcat and Kcat/ Km of enzyme for cypermethrin were 1.236 ⧎mol/min, 6.418 ⧎mol/mL, 13.493 min and 2.102 mL/mol.min, respectively. The degradation products of cypermethrin were identified using gas chromatographmass spectrometric (GC-MS) analysis. The degradation pathway followed by HU-S-01 involves oxidative as well as hydrolyzing. Biodegradation ability of strain Streptomyces sp. HU-S-01 without toxic byproducts reveals its potential for further study as a biological agent for the remediation of soil, water or crops, contaminated with cypermethrin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Explore related subjects

Discover the latest articles, news and stories from top researchers in related subjects.

References

  • Agarry, S. E.; Solomon, B. O., (2008). Kinetics of batch microbial degradation of phenols by indigenous pseudomonas fluorescence. Int. J. Environ. Sci. Tech., 5(2), 223–232 (10 pages).

    Article  CAS  Google Scholar 

  • Agarry, S. E.; Audu, T. O. K.; Solomon, B. O., (2009). Substrate inhibition kinetics of phenol degradation by pseudomonas fluorescence from steady state and wash-out data. Int. J. Environ. Sci. Tech., 6(3), 443–450 (8 pages).

    Article  CAS  Google Scholar 

  • Alisa, S. V.; Wansiri, P., (2007). Biodegradation of 4-chloroaniline by bacteria enriched from soil. FEMS Microbiol. Lett., 268, 209–216 (8 pages).

    Article  Google Scholar 

  • Arulazhagan, P.; Vasudevan, N.; Yeom, I. T., (2010). Biodegradation of polycyclic aromatic hydrocarbon by a halotolerant bacterial consortium isolated from marine environment. Int. J. Environ. Sci. Tech., 7(4), 839–852 (14 pages).

    Google Scholar 

  • Ashwani, S.; Indu, S. T.; Prem, D., (2009). Enrichment, isolation and characterization of pentachlorophenol degrading bacterium Acinetobacter sp. ISTPCP-3 from effluent discharge site. Biodegradation, 20(5), 643–650 (8 pages).

    Article  Google Scholar 

  • Babel, S.; Opiso, E. M., (2007). Removal of Cr from synthetic wastewater by sorption into volcanic ash soil. Int. J. Environ. Sci. Tech., 4(1), 99–108 (10 pages).

    Article  CAS  Google Scholar 

  • Bradford, M. M., (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248–254 (7 pages).

    Article  CAS  Google Scholar 

  • Cabral, J. R.; Galendo, D.; Laval, M.; Lyandrat, N., (1990). Carcinogenicity studies with deltamethrin in mice and rats. Cancer. Lett., 49(2), 147–152 (6 pages).

    Article  CAS  Google Scholar 

  • Cabral, J. R.; Galendo, D., (1990). Carcinogenicity study of the pesticide fenvalerate in mice. Cancer. Lett., 49(1), 13–1 (13 pages).

    Article  CAS  Google Scholar 

  • Chen, M.; Ren, R.; Wang, Z. J.; Lin, X.T.; Zhang, S. F., (2007). Residues of pyrethroid pesticides in wastewater of the sewage treatment plant. Environ. Moni. Chin., 23(1), 27–30 (4 pages).

    Google Scholar 

  • Cuthbertson, A. G. S.; Blackburn, L. F.; Northing, P.; Luo, W.; Cannon, R. J. C.; Walters, K. F. A., (2010). Chemical compatibility testing of the entomopathogenic fungus lecanicillium muscarium to control bemisia tabaci in glasshouse environment. Int. J. Environ. Sci. Tech., 7(2), 405–409 (5 pages).

    Article  CAS  Google Scholar 

  • Cuthbertson, A. G. S.; Murchie, A. K., (2010). Ecological benefits of Anystis baccarum in an orchard ecosystem and the need for its conservation. Int. J. Environ. Sci. Tech., 7(4), 807–813 (7 pages).

    Google Scholar 

  • Dhanasekaran, D.; Thajuddin, N.; Rashmi, M.; Deepika, T. L.; Gunasekaran, M., (2009). Screening of biofouling activity in marine bacterial isolate from ship hull. Int. J. Environ. Sci. Tech., 6(2), 197–202 (6 pages).

    CAS  Google Scholar 

  • Dorman, D. C.; Beasley, V. R., (1991). Neurotoxicity of pyrethrin and pyrethroid insecticides. Vet. Hum. Toxicol., 33(3), 238–243 (6 pages).

    CAS  Google Scholar 

  • Dua, M.; Singh, A.; Sethunathan, N.; Johri, A. K., (2002). Biotechnology and bioremediation: successes and limitations. Appl. Microbiol. Biotechnol., 59, 143–152 (10 pages).

    Article  CAS  Google Scholar 

  • Edward, T.; Akhtar, M., (1991). Identification and Characterization of a Pseudomonas Strain Capable of Metabolizing Phenoxybenzoates. Appl. Environ. Microbiol., 57(5), 1294–1300 (7 pages).

    Google Scholar 

  • Elliott, M., (1980). Established pyrethroid insecticides. Pestic. Sci., 11, 119–128 (10 pages).

    Article  CAS  Google Scholar 

  • Ghasemi, F.; Tabandeh, F.; Bambai, B.; Rao, K. R. S. S., (2010). Decolorization of different azo dyes by Phanerochaete chrysosporium RP78 under optimal condition. Int. J. Environ. Sci. Tech., 7(3), 457–464 (8 pages).

    Article  CAS  Google Scholar 

  • Grant, R. J.; Daniell, T. J.; Betts, W. B., (2002). Isolation and identification of synthetic pyrethroid-degrading bacteria. J. Appl. Microbiol., 92(3), 534–540 (7 pages).

    Article  CAS  Google Scholar 

  • Halden, R. U.; Tepp, S. M.; Halden, B. G.; Dwyer, D. F., (1999). Degradation of 3-phenoxybenzoic acid in soil by Pseudomonas pseudoalcaligenes POB31 0 (pPOB) and two modified Pseudomonas strains. Appl. Environ. Microbiol., 65(8), 3354–3359 (6 pages).

    CAS  Google Scholar 

  • Horne, I.; Sutherland, T. D.; Harcourt, R. L.; Russell, R. J.; Oakeshott, J. G., (2002). Identification of an OPD (organophosphate degradation) gene in an Agrobacterium isolate. Appl. Environ. Microbiol., 68(7), 3371–3376 (6 pages).

    Article  CAS  Google Scholar 

  • Hosseini, F.; Malekzadeh, F.; Amirmozafari, N.; Ghaemi, N., (2007). Biodegradation of anionic surfactants by isolated bacteria from activated sludge. Int. J. Environ. Sci. Tech., 4(1), 127–132 (6 pages).

    Article  CAS  Google Scholar 

  • Jilani, S.; Altaf Khan, M., (2006). Biodegradation of cypermethrin by pseudomonas in a batch activated sludge process. Int. J. Environ. Sci. Tech., 3(4), 371–380 (10 pages).

    Article  CAS  Google Scholar 

  • Kakko, I.; Toimela, T.; Tahti, H., (2004). Oestradiol potentiates the effects of certain pyrethroid compounds in the MCF7 human breast carcinoma cell line. Altern. Lab. Anim., 32(4), 383–390 (8 pages).

    CAS  Google Scholar 

  • Katsuda, Y., (1999). Development of and future prospects for pyrethroid chemistry. Pestic. Sci., 55(8), 775–782 (8 pages).

    Article  CAS  Google Scholar 

  • Kim,Y. M.; Jean,J. R.; Murugesan, K.; Kim, E. J.; Chang, Y. S., (2009). Biodegradation of 1, 4-dioxane and transformation of related cyclic compounds by a newly isolated Mycobacterium sp.PH-06. Biodegradation, 20(4), 511–519 (9 pages).

    Article  CAS  Google Scholar 

  • Liang, W. Q.; Wang, Z. Y.; He, L.; Wu, P. C.; Hu, J. M.; Luo, N. X.; Li, C.; Liu, Y. H., (2005). Purification and characterization of a novel pyrethroid hydrolase from Aspergillus niger ZD11. J. Agric. Food. Chem., 53(19), 7415–7420 (6 pages).

    Article  CAS  Google Scholar 

  • Litchfield, M. H., (1985).Toxicity to mammals, in: J.P. Leahay (editor), The Pyrethroid Insecticides. Taylor & Francis, London, 99–150 (52 pages).

    Google Scholar 

  • Liu, F.; Zhong, Y. C., (2000). Screening of a dimethoate-degrading enzyme producing strain and it’s optimization of the enzyme production. J. Agro-Environ. Sci., 19(6), 336–338 (3 pages).

    Google Scholar 

  • Maloeny, S. E.; Maule, A.; Smith, A. R.W., (1992). Transformation of synthetic pyrethroid insecticides by a thermophilic Bacillus sp. Arch Microbiol., 158, 282–286 (5 pages).

    Article  Google Scholar 

  • Maloeny, S. E.; Maule, A.; Smith, A. R.W., (1993). Purification and preliminary characterization of permethrinase from a pyrethroid-transforming strain of Bacillus cereus. Appl. Environ. Mocrobiol., 59(7), 2007–2013 (7 pages).

    Google Scholar 

  • Mandar, P.; Manish, J.; Dileep, D., (2005). Biodegradation of allethrin, a pyrethroid insecticide, by an Acidomonas sp. Biotechnol. Lett., 27(23-24), 1909–1913 (5 pages).

    Article  Google Scholar 

  • Murugesan, A. G.; Jeyasanthi, T.; Maheswari, S., (2010). Isolation and characterization of cypermethrin utilizing bacteria from brinjal cultivated soil. Afr. J. Microbiol. Res., 4(1), 10–13 (4 pages).

    Google Scholar 

  • Nirmali, S.; Subrata, K. D.; Bharat, K. C.; Patel, R. N.; Aqbal, S.; Madhuban, G., (2005). Biodegradation of beta-cyûuthrin by Pseudomonas stutzeri strain S1. Biodegradation, 16(6), 581–589 (8 pages).

    Article  Google Scholar 

  • Nwuche, C. O.; Ugoji, E. O., (2008). Effects of heavy metal pollution on the soil microbial activity. Int. J. Environ. Sci. Tech., 5(3), 409–414 (6 pages).

    Article  CAS  Google Scholar 

  • Nwuche, C. O.; Ugoji, E. O, (2010). Effect of co-existing plant specie on soil microbial activity under heavy metal stress. Int. J. Environ. Sci. Tech., 7(4), 697–704 (7 pages).

    CAS  Google Scholar 

  • Pazos, F.; Valencia, A.; De Lorenzo, V, (2003). The organization of the microbial biodegradation network from a systems-biology perspective. EMBO Rep., 4(10), 994–999 (6 pages).

    Article  CAS  Google Scholar 

  • Pearce, F., (1997). Sheep dips poison river life. New Sci., 153, 4.

    Google Scholar 

  • Sakata, S.; Mikami, N.; Yamada, H., (1992). Degradation of pyrethroid optical isomers by soil microorganisms. J. Pestcide Sci., 17(3), 181–189 (9 pages).

    Article  Google Scholar 

  • Schmid, A.; Dordick, J. S.; Hauer, B.; Kiener, A.; Wubbolts, M.; Witholt, B., (2001). Industrial biocatalysis today and tomorrow. Nature, 409, 258–268 (11 pages).

    Article  CAS  Google Scholar 

  • Shen, C. L.; Fan, Z. X.; Zhu, Q. S., (2006). Residual analysis of cypermethrin in corn and soil. J. Qingdao. Univ. Sci. Tech., 27(3), 202–206 (5 pages).

    CAS  Google Scholar 

  • Shukla, Y.; Yadav, A.; Arora, A., (2002). Carcinogenic and cocarcinogenic potential of cypermethrin on mouse skin. Cancer Lett., 182(1), 33–41 (9 pages).

    Article  CAS  Google Scholar 

  • Stock, J. E.; Huang, H. Z.; Jones, P. D.; Wheelock, C. E.; Morisseau, C.; Hammock, B. D., (2004). Identification, expression, and purification of a pyrethroid-hydrolyzing carboxylesterase from mouse liver microsomes. J. Biol. Chem., 279(28), 29863–29869 (7 pages).

    Article  Google Scholar 

  • Tallur, P. N.; Megadi,V. B.; Ninnekar, H. Z., (2008). Biodegradation of Cypermethrin by Micrococcus sp. Strain CPN 1. Biodegradation, 19(1), 77–82 (6 pages).

    Article  CAS  Google Scholar 

  • Tyler, C. R.; Beresford, N. M.; Sumpter, J. P., (2000). Metabolism and environmental degradation of pyrethroid insecticides produce compounds with endocrine activities. Environ. Toxicol. Chem., 19(4), 801–809 (9 pages).

    Article  CAS  Google Scholar 

  • Vinodhini, R.; Narayanan, M., (2008). Bioaccumulation of heavy metals in organs of fresh water fish Cyprinus carpio (Common carp). Int. J. Environ. Sci. Tech., 5(2), 179–182 (4 pages).

    Article  CAS  Google Scholar 

  • Virtue, W. A.; Clayton, J. W., (1997). Sheep dip chemicals and water pollution. Sci. Total Environ., 194, 207–217 (11 pages).

    Article  Google Scholar 

  • Wang, B. Z.; Guo, P.; Hang, B. J.; Li, L.; He, J.; Li, S. P., (2009). Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from Sphingobium sp. strain JZ-1 and characterization of the gene product. Appl. Environ. Microbiol., 75(17), 5496–5500 (5 pages).

    Article  CAS  Google Scholar 

  • Wang, L.; Lei, J.; Xiang, W. L.; Jiang, S. Y.; Sun, J. R.; Yang, Z. R., (2009 ). Identification and characterization of purple pigment-producing actinomycete Strain. Chin J. Appl. Environ. Biol., 15(1), 139–142 (4 pages).

    CAS  Google Scholar 

  • Wu, P. C.; Liu, Y. H.; Wang, Z. Y.; Li, H.; Liang, W. Q.; Luo, N.; Hu, J. M.; Zhang, X. Y.; Lu, J. Q.; Luan, T. G.; Cao, L. X., (2006). Molecular cloning, purification and biochemical characterization of a novel pyrethroid-hydrolyzing esterase from Klebsiella sp. strain ZD112. J. Agric. Food. Chem., 54(3), 836–842 (7 pages).

    Article  CAS  Google Scholar 

  • Xie, W. J.; Zhuo, J. M.; Wang, H. Y.; Chen, X. Q., (2007). Determination of — phenoxybenzoic acid in soil using HPLC. J. Agro-Environ. Sci., 26(2), 608–611 (4 pages).

    CAS  Google Scholar 

  • Yan, X.C, (1992). Classification and identification of Actinomycetes, Beijing Science Press., Beijing, 265–1045

    Google Scholar 

  • Yousefi Kebria, D.; Khodadadi, A.; Ganjidoust, H.; Badkoubi, A.; Amoozegar, M. A., (2009). Isolation and characterization of a novel native Bacillus strain capable of degrading diesel fuel. Int. J. Environ. Sci. Tech., 6(3), 435–442 (8 pages).

    Article  Google Scholar 

  • Zhao, W. X.; Gong, D. X.; Yang, R. B.; Li, Y. W.; Guo, Z. Y.; Zou, Y. Z., (2005). Dynamics of cypermethrin residue in litchi fruit and soil. Ecol. Sci., 24(4), 350–354 (5 pages).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, 510640, P. R. China

    Q. S. Lin Ph.D., M. Y. Hu (Professor), M. R. Ul Haq Ph.D., L. Yang (M.Sc. Candidate) & H. Li (M.Sc. Candidate)

  2. Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou, 510642, P. R. China

    S. H. Chen (Ph. D. Scholar)

Authors
  1. Q. S. Lin Ph.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. H. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Y. Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. R. Ul Haq Ph.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. H. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Y. Hu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lin, Q.S., Chen, S.H., Hu, M.Y. et al. Biodegradation of Cypermethrin by a newly isolated actinomycetes HU-S-01 from wastewater sludge. Int. J. Environ. Sci. Technol. 8, 45–56 (2011). https://doi.org/10.1007/BF03326194

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03326194

Keywords

Search

Navigation