[go: up one dir, main page]
Skip to main content
SpringerLink
Log in

Detoxification of Atrazine by Endophytic Streptomyces sp. Isolated from Sugarcane and Detection of Nontoxic Metabolite

  • Published:
Bulletin of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

Atrazine is still one of the most used agricultural pesticides worldwide and it has been recognized as a major contaminant of surface and ground water. The aims of this research were to isolate an endophytic microorganism from leaves of sugarcane, evaluate its ability to degrade atrazine, and investigate the formation of metabolites. By sequencing of the 16S rRNA gene, the endophytic isolate atz2 was identified as Streptomyces sp. The reduction in atrazine concentration by Streptomyces sp. atz2 was 98 % and UHPLC-MS/MS analyses showed the appearance of an unknown metabolite observed as m/z 311. Ecotoxicity tests with an aquatic organism, Daphnia similis, confirmed that this metabolite was nontoxic. This mechanism of detoxification of atrazine is different from the ones of other free-living microorganisms that inhabit the soil or rhizosphere. The results show new aspects of atrazine detoxification, highlighting a new role of endophytic bacteria in plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Explore related subjects

Discover the latest articles, news and stories from top researchers in related subjects.

References

  • ABNT (Brazilian Association of Technical Norms) (2004) N-BR 12713—water ecotoxicology—acute toxicity—assay with Daphnia spp. (Cladocera, Crustacea). Rio de Janeiro

  • Boundy-Millis KL, Souza ML, Mandelbaum RT, Wackett LP, Sadowsky MJ (1997) The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway. Appl Environ Microbiol 63:916–923

    Google Scholar 

  • Kiely T, Donaldson D, Grube A (2004) Pesticides industry sales and usage: 2000 and 2001 market estimates. USEPA, Washington, DC

    Google Scholar 

  • Krutz LJ, Shaner DL, Accinelli C, Zablotowicz RM, Henry WB (2008) Atrazine dissipation in s-triazine-adapted and non adapted soil from Colorado and Mississippi: implications of enhanced degradation on atrazine fate and transport parameters. J Environ Qual 37:848–857

    Article  CAS  Google Scholar 

  • Krutz LJ, Shaner DL, Weaver MA, Webb RMT, Zablotowicz RM, Reddy KN, Huang Y, Thomson SJ (2010a) Agronomic and environmental implications of enhanced s-triazine degradation. Pest Manag Sci 66:461–481

    Article  Google Scholar 

  • Krutz LJ, Shaner DL, Zablotowicz RM (2010b) Enhanced degradation and soil depth effects on the fate of atrazine and major metabolites in Colorado and Mississippi soils. J Environ Qual 39:1369–1377

    Article  CAS  Google Scholar 

  • Magnani GS, Didonet CM, Cruz LM, Picheth CF, Pedrosa FO, Souza EM (2010) Diversity of endophytic bacteria in Brazilian sugarcane. Genet Mol Res 9:250–258

    Article  CAS  Google Scholar 

  • McGuiness M, Dowling D (2009) Plant-associated bacterial degradation of toxic organic compounds in soil. Int J Environ Res Public Health 6:2226–2247

    Article  Google Scholar 

  • Melnikov P, Freitas TCM (2011) Evaluation of acute chromiun (III) toxicity in relation to Daphnia similis. J Water Res Prot 3:127–130

    Article  CAS  Google Scholar 

  • Oliveira PFL, Vasconcellos SP, Angolini CFF, Cruz GF, Marsaioli AJ, Santos Neto EV, Oliveira VM (2012) Taxonomic diversity and biodegradation potential of bacteria isolated from oil reservoirs of an offshore southern Brazilian basin. J Petrol Environ Biotechnol 3:1–14

    Google Scholar 

  • Pathak RK, Dikshit AK (2012) Atrazine and its use. Int J Res Chem Environ 2:1–6

    CAS  Google Scholar 

  • Shimabukuro RH (1967) Atrazine metabolism and herbicidal selectivity. Plant Physiol 42:1269–1276

    Article  CAS  Google Scholar 

  • Velázquez-Fernández JB, Martínez-Rizo AB, Ramírez-Sandoval M, Domíngues-Ojeda D (2012) Pesticides - Recent Trends in Pesticide Residue Assay. In: Soundararajan RP (ed) Biodegradation and bioremediation of organic pesticides. Agricultural and biological sciences. http://dx.doi.org/10.5772/46845

  • Wackett LP, Sadowsky MJ, Martinez B, Shapir N (2002) Biodegradation of atrazine and related s-triazine compounds: from enzymes to field studies. Appl Microbiol Biotechnol 58:39–45

    Article  CAS  Google Scholar 

  • Weyens N, van der Lelie D, Taghavi S, Vangronsveld J (2009) Phytoremediation: plant-endophyte partnerships take the challenge. Curr Opin Biotechnol 20:248–254

    Article  CAS  Google Scholar 

  • Yassir A, Lagacherie B, Hout S, Soulas G (1999) Microbial aspects f atrazine biodegradation in relation to history of soil treatment. Pest Sci 55:799–809

    Article  CAS  Google Scholar 

  • Young A, Blakesley R (1991) Sequencing plasmids from single colonies with the dsDNA cycle sequencing system. Focus 13:13

    Google Scholar 

Download references

Acknowledgments

The authors would like to thank Angelis, DF and Oliveira, VJA for the ecotoxicity tests, Mazzafera, P for the use of the UHPLC-MS equipment (FAPESP/BIOEN Grant 08/58035-6), Eberlin, MN for the use of the a 7.2T LTQ FT ultra mass spectrometer, Beati, AAGF and Vendramini, CMM for the statistical analysis. This research was supported by São Paulo Research Foundation (FAPESP Grant 11/50917-2).

Author information

Authors and Affiliations

  1. Research Laboratory in Applied Microbiology, São Francisco University, Bragança Paulista, SP, Brazil

    Josiane A. Mesquini & Natalia R. S. Miyasaka

  2. Department of Plant Biology, Institute of Biology, State University in Campinas (UNICAMP), Campinas, SP, Brazil

    Alexandra C. H. F. Sawaya & Begonã G. C. López

  3. Research Center for Chemistry, Biology and Agriculture, State University in Campinas (UNICAMP), Campinas, SP, Brazil

    Valéria M. Oliveira

Authors
  1. Josiane A. Mesquini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alexandra C. H. F. Sawaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Begonã G. C. López
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Valéria M. Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Natalia R. S. Miyasaka
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Natalia R. S. Miyasaka.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mesquini, J.A., Sawaya, A.C.H.F., López, B.G.C. et al. Detoxification of Atrazine by Endophytic Streptomyces sp. Isolated from Sugarcane and Detection of Nontoxic Metabolite. Bull Environ Contam Toxicol 95, 803–809 (2015). https://doi.org/10.1007/s00128-015-1673-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00128-015-1673-7

Keywords

Search

Navigation