Ibrahim Sani
Kebbi State University of Science And Technology, Biochemistry, Faculty Member
- Dr. Ibrahim Sani (BSc Biochemistry, Bayero University, Kano, Nigeria; MSc Biochemical Engineering, University of Birm... moreDr. Ibrahim Sani (BSc Biochemistry, Bayero University, Kano, Nigeria; MSc Biochemical Engineering, University of Birmingham, UK; PhD Biochemistry, Usmanu Danfodio University, Sokoto, Nigeria) is an Assistant Professor at the Department of Biochemistry, Faculty of Life Sciences, Kebbi State University of Science and Technology, Aliero, Nigeria. He has working experience in University lecturing, research and community service since 2009. He is involved in many Academic and Administrative activities of the University. His area of specialization (Research Interest) is Pharmacological Biochemistry and Ethnopharmacology. He has supervised many students’ research works at both undergraduate and postgraduate levels. He has published many research pepers in reputable journals within and outside Nigeria and has attended/participated in many conferences, seminars, workshops and trainings and presented papers at National and International levels. Dr. Ibrahim Sani is a member of many professional societies, such as, Science Association of Nigeria (SAN); Nigerian Society of Biochemistry and Molecular Biology (NSBMB); Society for Basic and Clinical Toxicology Nigeria (SBCTN); Society for Medicinal Plants and Economic Development (SOMPED); International Society for Ethnopharmacology (ISE); etc. Dr. Ibrahim Sani is currently leading Research Groups in the following Research Projects:
1. Development of Phyto-Antisnake Venom from Local Medicinal Plants as Potential Alternative to Serum-Based Antivenins. 2. Development of Plant-Based and Broad-Spectrum Antibacterial Agents as Alternative for Resolving Bacterial Antibiotic Resistance. 3. Screening and Formulation of Local Medicinal Plants for the Management of Oxidative Stress. 4. Assessment of Medicinal and Economic Profiles of Some Essential Oil Extracts from Local Medicinal Plants. 5. Risk Assessment Profiles of Locally Used Medicinal Plants. 6. Development of Potential Vaccine against Snakebite Envenomation. His outdoor activities is mainly Guidance and Counseling. His hobbies are mentoring, watching movies and news, strolling and traveling. He is happily married with children.edit
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Bacterial infections are among the major causes of morbidity and mortality worldwide. The development of drug resistance to most antibiotics by bacterial species are pandemic, these necessitate the urgent need to search for new... more
Bacterial infections are among the major causes of morbidity and mortality worldwide. The development of drug resistance to most antibiotics by bacterial species are pandemic, these necessitate the urgent need to search for new antibacterial drugs from the natural sources. Hence this research was aimed at evaluating the antibacterial activity of some medicinal plants on the most commonly drug resistant bacterial isolates from some patients attending Federal Medical Center (FMC) Birnin Kebbi, Nigeria. An ethno-botanical survey was carried out within Zuru Emirate and three (3) plant samples (Lonchocarpus laxiflorus root, Mitragyna inermis root and Lawsonia inermis root) were selected based on their number of citations and limited number of reported researches on the antibacterial activities of their roots. The multidrug resistant bacterial strains (Staplococcus aureus, Escherichia coli and Psuedomonas aeruginosa) obtained from FMC, Birnin Kebbi, were taken from nutrient agar slant and...
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Microorganisms play an integral role in detoxification and removal of toxic compounds from the environment. Aniline is the simplest aromatic amine, consisting of a phenyl group attached to an amino group that is used as herbicide to... more
Microorganisms play an integral role in detoxification and removal of toxic compounds from the environment. Aniline is the simplest aromatic amine, consisting of a phenyl group attached to an amino group that is used as herbicide to control weeds. Aniline is detrimental to both environment and health. In this research, six previously isolated bacteria (isolate A-F) were screened on Bushnell Hass media for their potential to grow and utilize aniline as a sole carbon source. Isolate A (Pseudomonas sp.) was found to tolerate and grow best with aniline sole source of carbon. Optimum conditions for aniline degradation by this isolate were found to be pH 6.0, temperature between 30 and 37 °C, inoculums size of 600 μL, aniline concentration of 200 mg/L and incubation time of 96 h. The capacity of this isolate to reduce toxic aniline to less toxic form is novel and makes the bacterium important instrument for bioremediation of this pollutant.
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One of the beneficial roles of the microbial population is their ability to convert toxic herbicides to lesser toxic compounds such as water and carbon (IV) oxide. Paraquat which is an acutely toxic herbicide is used on farmlands and has... more
One of the beneficial roles of the microbial population is their ability to convert toxic herbicides to lesser toxic compounds such as water and carbon (IV) oxide. Paraquat which is an acutely toxic herbicide is used on farmlands and has been found to affect human health. This study was aimed at characterizing bacteria with the potential to degrade paraquat. Previously isolated bacteria from culture collection labelled A-F were screened for their potential to degrade and utilized paraquat as the sole carbon source in Bushnell Hass agar media. Of the six isolates, isolate E (Morganella sp.) was observed to have the highest growth and tolerance to paraquat after 72 h of incubation at 37 ºC. Characterization study revealed that Morganella sp. can utilize and grow with optimum conditions of pH 6.5, the temperature of 30 ºC and can tolerate up to 400 mg/L paraquat concentration with an increase in growth as inoculum size increases. Thus, these findings showed that Morganella sp. can degr...
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There are more than 400 known species of edible insects. Many species of these insects have been used as traditional foods among various communities. Insects have played an important part in the history of human nutrition in Africa. Thus... more
There are more than 400 known species of edible insects. Many species of these insects have been used as traditional foods among various communities. Insects have played an important part in the history of human nutrition in Africa. Thus the aim of this research is to evaluate the nutritional value and mineral composition of edible Zonocerus variegatus (grasshopper). To evaluate the nutritional value, proximate, antinutrient and mineral elemental composition were analyzed using standard methods. The result of the proximate analysis revealed the following: Moisture (5.33±1.16%), Ash (11.50±3.04%), Crude lipid (49.33± 2.08%), Crude fiber (2.03±1.01%), Protein (2.19±0.87%) and Carbohydrate content (29.61±4.21%). The concentrations (mg/100g) of the antinutrients in this edible insect are; 11.25±0.46 for oxalate, 2.18±0.24 for phytate and 3.90 ±0.1 for tannin. The mineral composition (mg/kg) revealed, Na, K, Ca, Mg, Fe, Cu and Zn with 0.02±0.00, 0.04± 0.01, 1.12± 0.07, 0.57±0.08, 1.84±0....
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Phytochemicals with protein binding properties, active against snake envenomation such as flavonoids, polyphenols, saponins, tannins, terpenoids, xanthenes, quinonoids, steroids and alkaloids bind to toxic venom proteins thereby... more
Phytochemicals with protein binding properties, active against snake envenomation such as flavonoids, polyphenols, saponins, tannins, terpenoids, xanthenes, quinonoids, steroids and alkaloids bind to toxic venom proteins thereby inactivating them. This research was aimed at isolating antisnake venom agents from Azadirachta indica leaf extracts using activity-guided isolation protocols. A. indica leaf was collected, authenticated and extracted using methanol followed by solvent-fractionation using hexane and ethylacetate. These fractions were further separated using column and thin-layer chromatography. The pooled chromatographic fractions were screened for the antivenom activity using Naja nigricollis venom phospholipase A2 (NVPLA2) inhibition assay and albino rat models. Polyvalent serum-based antivenin was used as standard. The column chromatography of the A. indica leaf hexane and ethylacetate extracts yielded sixty (60) and sixty nine (69) fractions. Based on similarities on the...
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Schistosomiasis is a parasitic disease that affect the well-being of people in Sokoto State-Nigeria. A survey of medicinal plants used in the treatment of schistosomiasis was conducted in Kware/Wammakko Local Government areas of the state... more
Schistosomiasis is a parasitic disease that affect the well-being of people in Sokoto State-Nigeria. A survey of medicinal plants used in the treatment of schistosomiasis was conducted in Kware/Wammakko Local Government areas of the state through the administration of structured questionnaire. The survey from 48 respondents revealed total 9 plant species belonging to different families were found to be frequently used in treatment of schistosomiasis. Qualitative and quantitative phytochemical analyses on the plants revealed the presence of secondary metabolites. In Acacia nilotica, flavvnoids (3.53 %), alkaloids (2.3 %), saponins (1.507 %), tannins (64 mg/dl), glycosides (0.32 g%), and steroids (79 mg%) were found to be present. In Balanites aegyptiaca, flavvnoids (2.66 %), alkaloids (2.6 %), saponins (1.05 %), tannins (72 mg/dl), glycosides (0.30 g%) and steroids (91 mg%) were present. In Khaya senegalensis, flavnoids (0.77 %), alkaloids (1.8 %), saponins (0.5 %), tannins (23 mg/dl...
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Background: Sickle cell disease is a genetic disorder in which an individual inherits the sickle cell allele from both parents. The modern disease modifying therapies are quite expensive and often come with side effects, hence, there is... more
Background: Sickle cell disease is a genetic disorder in which an individual inherits the sickle cell allele from both parents. The modern disease modifying therapies are quite expensive and often come with side effects, hence, there is need to search for natural alternatives from medicinal plants. This research was aimed at evaluating the antisickling effects of some selected medicinal plants. Materials and Methods: Ethnobotanical survey was conducted on the medicinal plants used in Zuru Local Government Area of Kebbi State, Nigeria for the treatment/management of sickle cell disease. Five (5) most cited plants; Carica papaya leaf, Prosopis africana stem-bark, Guiera senegalensis leaf, Syzygium aromaticum seed and Boswellia dalzielli stem bark were selected and their methanol extracts were subjected to in vitro antisickling activity using sodium metabisulphite. Phytochemical screening on the most active plant extracts was conducted using standard methods. Results: The plant extrac...
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Several studies have been reported on active peptides isolated from some medicinal plants, which were effective inhibitors against snake venom induced toxicities. Hence, the aim of this research work was to isolate, purify and... more
Several studies have been reported on active peptides isolated from some medicinal plants, which were effective inhibitors against snake venom induced toxicities. Hence, the aim of this research work was to isolate, purify and characterize an antisnake venom plant peptide from Bauhinia rufescens seed that can serve as potential alternative to serum-based antivenins. B. rufescens seed was collected, duly identified, authenticated and processed. The peptide was isolated from the seed and purified using gel filtration chromatography and SDS-PAGE and then named as BRS-P19. Venom Phospholipase A2 (VPLA2) was used for the study and was isolated from Naja nigricollis venom. Albino mice of both sexes were used for in vivo experiments. They were divided into seven (7) groups of three (3) mice each. Group 1 served as normal control, group 2 were injected with VPLA2 only, group 3 and 4 were injected with VPLA2 then treated with BRS-P19 at doses of 0.2 and 0.4 mg/kg b.w. respectively, while mic...
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considering its high consumption, this work was carried out to determine the potential applications of the seed kernel by investigating the physicochemical characteristics as well as the phytochemical and mineral compositions of its... more
considering its high consumption, this work was carried out to determine the potential applications of the seed kernel by investigating the physicochemical characteristics as well as the phytochemical and mineral compositions of its extracted oil content. The oil was extracted using Soxhlet apparatus, and the physicochemical characterization, together with the phytochemical screening and determination of the mineral composition were carried out using standard methods. The physicochemical parameters of the extracted oil were as follows: acid value 0.7963 ± 0.21 mg KOH/g, saponification value 7.74 ± 0.73mg KOH/g, iodine value 46.8 ±0.63 I2/100g, free fatty acid 20.49±1.46 ( % oleic acid), peroxide value (mEqH2O2/100g) 10.0 ± 5.0, specific gravity (g) 0.95.0.05.The oil was observed to have clear white appearance, liquid at room temperature and has a nutty smell when fresh and unpleasant when rancid. Cocosnucifera L. seed kernel has low oil content of 26.61%. The phytochemical analysis ...
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Phytochemicalsare active secondary plant metabolites responsible for most of the claimed medicinal activities of plants. Eucalyptus camaldulensis is one of those plants that possess these phytochemicals and claimed to possess medicinal... more
Phytochemicalsare active secondary plant metabolites responsible for most of the claimed medicinal activities of plants. Eucalyptus camaldulensis is one of those plants that possess these phytochemicals and claimed to possess medicinal activities on various ailments. The phytochemicals constituents of various parts of this plant were investigated using standard methods of phytochemicals screening in both aqueous and ethanolic extracts. Qualitative screening revealed that tannins, saponins, glycosides, steroids and anthraquinones were present in aqueous extract of all the parts of the plant, whereas alkaloids, flavonoids and terpenoids were absent. On the other hand, tannins and steroids were present in the ethanolic extract of all the parts of the plant, while saponins, alkaloids, flavonoids and terpenoids were present only in some parts of the plant. However, glycosides and anthraquinone were absent in all the ethanolic extracts. The quantitative screening revealed large amount of ...
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Due to the abundance of Cocosnucifera L. (coconut) in southern part of Nigeria and considering its high consumption, this work was carried out to determine the potential applications of the seed kernel by investigating the physicochemical... more
Due to the abundance of Cocosnucifera L. (coconut) in southern part of Nigeria and considering its high consumption, this work was carried out to determine the potential applications of the seed kernel by investigating the physicochemical characteristics as well as the phytochemical and mineral compositions of its extracted oil content. The oil was extracted using Soxhlet apparatus, and the physicochemical characterization, together with the phytochemical screening and determination of the mineral composition were carried out using standard methods. The physicochemical parameters of the extracted oil were as follows: acid value 0.7963± 0.21 mg KOH/g, saponification value 7.74 ± 0.73mg KOH/g, iodine value 46.8 ±0.63 I2/100g, free fatty acid 20.49±1.46 (% oleic acid), peroxide value (mEqH2O2/100g) 10.0 ± 5.0, specific gravity (g) 0.95 .0.05.The oil was observed to have clear white appearance, liquid at room temperature and has a nutty smell when fresh and unpleasant when rancid. Cocos...
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The lethality of snake venom is mainly attributed to its phospholipase A2 component that hydrolyzes cellular phospholipids, leading to the release of arachidonic acid that generates potentially toxic reactive oxygen species (ROS).... more
The lethality of snake venom is mainly attributed to its phospholipase A2 component that hydrolyzes cellular phospholipids, leading to the release of arachidonic acid that generates potentially toxic reactive oxygen species (ROS). Imbalance between excessive generation and poor removal of ROS causes lipid peroxidation leading to cellular damage. Hence, this research was aimed at evaluating the antioxidant-enhancing effect of Azadirachta indica leaf fractionated extracts on Naja nigricollis venom in albino rats. A. indica leaf was collected, authenticated and extracted using 95% methanol followed by fractionation using hexane and ethyl acetate. Ferric Original Research Article Sani et al.; JOCAMR, 10(3): 52-63, 2020; Article no.JOCAMR.60586 53 reducing antioxidant power assay was used for the in vitro test, while, in vivo experiments were conducted using Albino rats. The in vitro antioxidant effect of the hexane and ethyl acetate fractions presented ferric reducing power of 68.80 ± 1...
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Some phytochemicals and mineral elements constituents of white Sesamum indicum seed oil were investigated. Oil was extracted from the white Sesamum indicum seed by the Soxhlet extraction method. The phytochemicals were analyzed using the... more
Some phytochemicals and mineral elements constituents of white Sesamum indicum seed oil were investigated. Oil was extracted from the white Sesamum indicum seed by the Soxhlet extraction method. The phytochemicals were analyzed using the standard methods of phytochemical analysis, and the elements were analyzed using an atomic absorption spectrophotometer by wet digest method. The oil has a percentage yield of 46.4%. The result of the qualitative phytochemical analysis revealed that the oil contains alkaloids, saponins, flavonoids, tannins, steroids, terpenoids, anthraquinone and phenols. Cardiac glycosides and phlobatannins are absent. The results of the quantitative analysis revealed that the oil contains alkaloids (132.80±0.15 mg/g), flavonoids (59.20±0.15 mg/g), saponins (42.80±0.12 mg/g), tannins (17.01±0.12 mg/g tannic acid equivalence) and total phenols (196.44±3.47 mg/g gallic acid equivalence). The result of the mineral element analysis revealed that 100 g of the oil contai...
The aim of this research was to produce Streptomyces-extracellular chitinase and screen its antifungal activity on a clinically isolated Candida albicans. The Streptomyces were isolated from an agricultural farmland; they were identified... more
The aim of this research was to produce Streptomyces-extracellular chitinase and screen its antifungal activity on a clinically isolated Candida albicans. The Streptomyces were isolated from an agricultural farmland; they were identified and screened for the chitinase production. Effects of time, temperature, pH and nitrogen sources on the chitinase production were determined using standard methods. Ammonium sulphate precipitation was used to partially purify the chitinase. Protein concentrations were determined spectrophotometrically using bovine serum albumin as standard. Agar-well diffusion method was used to evaluate the antifungal activity of the chitinase on C. albicans. The isolated Streptomyces were of three (3) strains, and all the strains are Gram positive, catalase positive, oxidase positive while, Strain A and C are indole positive and only Strain B is citrate positive. The maximum chitinase production was at 72 h, 40°C and when yeast extract was used as the nitrogen sou...
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In this research, hexane and acetone extracts of Bauhinia rufences, Securidaca longepedunculata and Ziziphus abyssinica were investigated for antibacterial activity. The phytochemical screening of the plants leaves extracts was conducted... more
In this research, hexane and acetone extracts of Bauhinia rufences, Securidaca longepedunculata and Ziziphus abyssinica were investigated for antibacterial activity. The phytochemical screening of the plants leaves extracts was conducted using standard methods of analyses. The effects of the two solvent extracts on the bacterial species (Escherichia coli, Psuedomonas aeruginosa, Salmonella typhi and Staphylococcus aereus) were determined using agar well diffusion method. The most susceptible microorganisms were P. aeruginosa, S. typhi while the least susceptible was E.coli. Highest antibacterial activity was observed with hexane extract of B. rufences against S. typhi (12.75mm). While minimum activity was observed with hexane extract of S. longepedunculata and acetone leaves extract of B. rufences against E. coli (6.00mm and 6.00mm respectively). Hexane extracts had more inhibitory effects compared to acetone extracts, but were less potent when compared to ampiclox used as standard ...
This research was designed to evaluate the antipyretic activity of K. senegalensis leaf extract with the view of isolating and identifying the active components. The K. senegalensis leaf was extracted using 90% methanol and further... more
This research was designed to evaluate the antipyretic activity of K. senegalensis leaf extract with the view of isolating and identifying the active components. The K. senegalensis leaf was extracted using 90% methanol and further fractionated with hexane, ethylacetate, n-butanol and distilled water.The qualitative phytochemical screening was carried out using standard methods.The crude extract and the fractions were screened for antipyretic activity using 15%w/v brewer’s yeast induced pyrexia on Albino rats. The components of the most active fraction were further separated using column and thin layer chromatographic techniques on silica gel. LD50 of the most active fraction was determined using probit analysis. The results of the phytochemical screening revealed the presence of tannins, phenols, steroids and cardiac glycosides in both the crude extract and its fractions. The crude extract at 400 mg/kg b.w. showed the highest antipyretic activity compared to the other doses tested....
Azadirachta indica A. Juss. (Neem) is a multipurpose medicinal plant, traditionally used in the treatment of various human ailments. This plant is tagged as having high toxicity profile, and the toxicity might be related to the polarity... more
Azadirachta indica A. Juss. (Neem) is a multipurpose medicinal plant, traditionally used in the treatment of various human ailments. This plant is tagged as having high toxicity profile, and the toxicity might be related to the polarity nature of the solvent used for extraction. Hence, this research was aimed at screening the toxicity profile of Azadirachta indica leaf fractionated extracts using Albino rats. A. indica leaf was collected, authenticated and extracted using 95 % methanol then fractionated with hexane and ethyl acetate. Median lethal dose (LD50) of each fraction was determined using single oral dose of 5,000 mg/kg body weight (b. wt.) to five (5) rats. For the sub-chronic toxicity screening, the fractions were administered to groups of rats at different concentrations. Group 1 served as control, groups 2-5 received 900, 1800, 2700 and 3600 mg/kg b. wt. respectively. After 28 days, biochemical indices of hepatic and renal functions as well as haematological and histopat...
Snake venom enzymes are the key substances involved in snake venom toxicity. Thus, inactivating these enzymes is generally considered to be the fundamental step in the management of snakebite. Conventionally, snakebite envenomation is... more
Snake venom enzymes are the key substances involved in snake venom toxicity. Thus, inactivating these enzymes is generally considered to be the fundamental step in the management of snakebite. Conventionally, snakebite envenomation is treated parenterally with serum-based antivenins, and adjuvants to these antivenins are required for maximum protection of victims. Hence, this research was aimed at evaluating the inhibitory effect of Azadirachta indica leaf extracts on Naja nigricollis Reinhardt venom enzymes and screens for their antivenom adjuvant effects. A. indica leaf was collected, authenticated and extracted using 95% methanol followed by fractionation using hexane and ethyl acetate. The venom enzymes inhibition assays was evaluated using in vitro methods, while, adjuvant effect was screened using Albino rats. The results revealed that both the hexane and the ethyl acetate fractions showed capability of inhibiting the venom enzymes significantly (P<0.05) when compared with ...