Kemal Ak

... Çalışmada İstanbul Üniversitesi Veteriner Fakültesi Dölerme ve Sun'i Tohumlama AnabilimDalında aynı bakım ve beslenme koşullarında barındırılan 3-5 ... Gil, J., Rodriguez-Irazoqui, M., Söderquist, L and Rodriguez-Martinez, H. Influence of centrifugation or low extension rates ...

Summary The purpose of this study was to reduce of early twin pregnancies to single pregnancies in the mares by crushing. Out of 671 pregnancies that were diagnosed by rectal ultrasonography 2-4 weeks after ovulation. 21 (3.11 %) twins were diagnosed. Vesicles were present in left horn in 16 (76.1 %) mares and in right horn in 5 (23.8%) mares. Although, twin pregnancies were successfully reduced to single pregnancies in 15 mares, both vesic­les resorbed in 2 (9.5%) marcs and vesicles developed normally even if crushed in 4 (19.0%) mares. Crushed twins rechecked on 5 days after crushing and persistent twin pregnancy terminated by prostaglandin F2 alfa in 4 mares. They re-mated in the first oestrus after prostaglandin injections to achieve normal pregnancy in the same breeding season. The success rate Tor crushing, day 16-22 embryos was %78.9 and % 42.8 for crushing day 22-28 embryos. Key Words: Twin pregnancy, mare, crushing. KISRAKLARDA ERKEN IKIZ GEBELIĞIN EZILEREK TEK GEBELIĞIN SA...

Animal production via SCNT provides a unique tool for protection of valuable individuals, conservation of vulnerable and endangered species and production of transgenic animals. A total of 167 MI and 219 MII stage oocytes were used as the material of the study. The oocytes were enucleated at 44 h after in vitro maturation by aspiration of the polar body and the MI or MII plates. Cycling granulosa cells were used for nuclear transfer. Cell fusion was induced with DC pulses of 2.0 kV/cm 60µs, 0.1s apart (2x) delivered by a BTX Electrocell Manipulator 200 (BTX, San Diego, CA, USA). After fusion, the embryos were activated by 1.0 kV/cm 20µs DC pulses 0.1s apart (2x) followed by 2 mM 6-DMAP (6-dimethylaminopurine) incubation in culture medium for 4 h in a humidified atmosphere of 5% CO2, 5% O2, and 90% N2 at 38°C. The somatic cell transferred embryos were cultured for 8 days in mSOF medium supplemented with 0.4% BSA in a humidified 5% CO2, 5% O2, and 90% N2 atmosphere at 38°C. After in v...

Ozet Calismada in vitro olgunlastirilmis kedi oositlerinin in vitro fertilize olabilme ve akabinde in vitro kultur ortaminda morula ve blastosist donemine ulasabilme kabiliyetlerinin arastirilmasi amaclandi. Arastirmanin materyalini, kisirlastirilmis sokak kedilerinin ovaryumlarindan kazanilan oositler olusturdu. Ovaryumlar iki saatlik sure icerisinde 38°C' deki PBS solusyonu icerisinde laboratuara tasindi. SOF medyumu icerisinde (%0.4 BSA+10µg/ml FSH + 10µg/ml LH), gaz karisimi (%5 0 2 ,%5 C0 2 ,%90 N 2 ) ve %100'e yakin nemin saglandigi 38.5°C'lik inkubator ortaminda 48 saat sure ile olgunlasmaya birakildi (IVM). Ardindan. Swim-Up yontemiyle kapasite edilen donmus kedi spermasiyla 20 saat in vitro fertilizasyona birakildi (IVF). In vitro olarak yedi gun sureyle kulture edilen oosit/embriyolar fikse edildi ve boyandi. Faz-kontrast mikroskopta x400 buyutmede gelisim durumlari saptandi. Calismada toplam 106 adet primer oosit kullanildi. In vitro olgunlastirilan bu oositle...

Ozet Calismada kisraklarda ureme mevsiminin farkli donemlerinde vaginal progesteron uygulamalarinin ostrus ve ovulasyonun uyarilmasina etkisi arastirildi. Tedavi gunune kadar ostrus gostermeyen 8-20 yas arasinda en az bir yil gebe kalmamis 136 safkan ingiliz kisrak kullanildi. Kisraklara 14 gun sureyle vaginal progesterone ve progesteron uygulamasinin bittigi gun (0. gun) tek doz PGF 2I± uygulandi. Uygulamalar 15 Şubat-15 Mart (n=59), 16 Mart-16 Nisan (n=49) ve 17 Nisan-30 Mayis (n=28) tarihlerinde yapildi. Tedavi sonrasi uygulama zamani ilerledikce istatistiksel olarak onemsiz de olsa ortalama ostrus gunu (3.54 ± 2.98; 2.90 + 2.47; 1.89 ± 1.25 gun) ve ovulasyon gunu (5.52 ± 3.62; 5.19 ± 2.79; 4.32 ± 2.09 gun) azaldi ve ostrus gosterme orani (%71.19; %85.71; %96.55) artti. Yine uygulama zamanina gore sirasiyla; gebelik oranlan %35.6; %42.6; %65.5 ve tedaviye cevap vermeyen kisraklarin orani %28.8; %14.3, %3.4 oldu. 17 Nisan sonrasi tedaviye alman grupta gebelik orani artti ve tedavi...

... Page 6. 34 Özen Banu ÖzdaĢ, Ümüt Cirit, Kamber Demir, Süleyman Bacınoğlu, Alper Baran, Serhat Pabuccuoğlu, ... Elde ettiğimiz sonuçları incelediğimizde, en iyi eritme sonrası embriyo gelişimi %52,2 ile Grup 1'de bulunurken en kötü sonuç ise %5 ile Grup 4'te saptanmıştır. ...

1 Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Istanbul University, 34320 Avcilar, Istanbul, Turkey 2 Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa, Turkey 3 ...

This study was performed 1) to compare different ratios of foetal calf serum (FCS) for in vitro maturation (IVM) of sheep oocytes and 2) to investigate different culture media and supplements for ovine embryo production in vitro. Primary oocytes collected from ovaries of slaughtered Kivircik ewes were divided into 2 groups randomly and incubated in vitro for 26 hours at 39°C in a humidified atmosphere of 5% CO2 in air, TCM 199 medium supplemented with 10g/ml follicle stimulating hormone, 10g/ml luteinizing hormone, 1 g/ml estradiol 17β and 10% FCS (low) or 20% FCS (high) was used as maturation medium. After in vitro maturation and fertilization, presumptive zygotes were again divided into 2 groups randomly, and co-cultured for 6 days with sheep oviductal epithelial cells. In the first culture group (TCM), TCM 199 medium supplemented with 55 sheep estrous serum (SES), and in the second group synthetic oviduct fluid (SOF) medium supplemented with 20% SES were used. The cleavage and...

Right heart infective endocarditis presenting as an isolated apical mural mass is an extremely uncommon finding. A 24-year-old woman with congenital deafness and aphasia was admitted with recurrent attacks of fever and lobar pneumonia. Her past medical history was significant for an open operation for left nephrolithiasis five months before admission. She had no history of congenital heart defect, intravenous drug use or central venous line insertion. Diagnostic workup revealed a large pedunculated solid mass attached to the apex of the right ventricle and multiple septic foci in both lungs. Repeated blood cultures were negative. In spite of aggressive antibiotic therapy, she had progressively worsening respiratory distress. She was successfully operated for the mass and the pathologic findings were consistent with endocarditis. To our knowledge, the anatomical location of the mural endocarditis (apex of right ventricle) is a pretty uncommon condition.

Abstract: The aim of the present study was to investigate the effects of gonadotrophin and Bovine Serum Albumin (BSA) supplemented Synthetic Oviduct Fluid (SOF) and Ham's F-10 media on the in vitro maturation of cat oocytes. Oocytes collected from spayed stray ...

Abstract: The objectives of this study were to (i) compare the effects of two staining techniques and one fixative solution on the morphological features of ram spermatozoa and (ii) evaluate the morphological features of dead/live spermatozoa by using Eosin-Nigrosin ...

Abstract: Cat semen was extended with 3% and 4% glycerol containing Tris extender and frozen in (0.25 ml) straws. Spermatological characteristics were examined in post-thaw semen samples. Five tom cats aged 2-3 were used. Semen was collected by electro-...

Summary: Five male dogs of various breeds at two years of age were used in the study. During the study, two ejaculates per week and total 50 ejaculates were collected from each male by digital manipulation technique. Spermatological evaluations as volume, motility, spermatozoa concentration, live spermatozoa rate, pH and morphologic examinations were carried out. Semen was divided into two equal

This is the very first report that suggests high pregnancy rates can be obtained with use of the Doublesynch protocol in anestrous dairy cows. Recently, a new synchronization method has been developed (Doublesynch) that resulted in synchronized ovulations both after the first and second gonadotropin-releasing hormone (GnRH) treatments. It was suggested that this protocol has the potential to increase the pregnancy rates in primiparous dairy cows. The aim of the current study was to confirm the success of the Doublesynch protocol and further to investigate the effect of this method on pregnancy rates in anestrous cows. Lactating primiparous Holstein (Bos taurus) cows (n=165) between 60 and 172 d postpartum were monitored twice with 10-d intervals (on Days -10 and 0) by ultrasonography, and blood samples were collected. Cows were classified as anestrous if both blood samples had progesterone (P4) concentration <1 ng/mL and as cyclic if at least one of the two samples had P4 concentration >or=1 ng/mL. Cyclic cows were classified again as cyclic-high P4 (having an active corpus luteum) if the second blood samples had P4 concentrations >or=1 ng/mL and as cyclic-low P4 if P4 concentrations were <1 ng/mL on Day 0. Then, the cows classified as anestrous (n=51), cyclic-high P4 (n=63), or cyclic-low P4 (n=51) were put into two treatment groups (Ovsynch or Doublesynch) randomly to establish six groups. Cows in the Ovsynch group were administered a GnRH (lecirelin 50 microg, im) on Day 0, PGF (Prostaglandin F2 alpha, D-cloprostenol 0.150 mg, im) on Day 7, and a second dose of GnRH 48 h later. Cows in the Doublesynch group were administered a PGF on Day 0, GnRH on Day 2, a second PGF on Day 9, and a second GnRH on Day 11. Timed artificial insemination (TAI) was performed 16 to 20 h after the second GnRH in both treatment groups. Pregnancy diagnosis was conducted (by ultrasonography) 45+/-5 d after TAI. In anestrous cows and those with high and low progesterone concentration at treatment onset, Doublesynch treatment led to markedly increased pregnancy rates with respect to Ovsynch treatment (P<0.05). On the overall analysis of data, it was revealed that the Doublesynch method increased pregnancy rates by 43 percentage units (29.8% vs. 72.8%, P<0.0001) in relation to Ovsynch. Pregnancy rates of cows having small, medium, or large follicles at the day of second GnRH administration were similar in the Doublesynch group (70.4%, 85.2%, and 63.0%, respectively; P>0.05), whereas pregnancy rates reduced dramatically as follicle size increased in the Ovsynch group, particularly in cows with follicles greater than 16 mm (45.5%, 28.1%, and 5.3%, respectively; P<0.05). Our results confirm and support observations that the Doublesynch protocol increases the pregnancy rates in postpartum primiparous cows as reported previously. Our data also demonstrate that the Doublesynch method increases the pregnancy rates in anestrous cows. Thus, these data suggest that the Doublesynch protocol can be used to obtain satisfactory pregnancy rates after TAI in both anestrous and cycling primiparous dairy cows regardless of stage of estrous cycle.

The influence of different extender osmolality levels and the presence of different cryoprotectants on the postthawed semen's characteristics and post-thawed plasma membrane integrity of ram spermatozoa were studied. Ram semen was frozen with TRIS-egg yolk based extender ...

... (LH pik), C3: Donuk Mavi=2.5-8 ng/ml., C4: Beyaz= >8 ng/ml. Çalışmada optimal tohumlama zamanı ... (Doktora Tezi), Ankara. 8. Keskin, O., Akçay, E., Kaya, M., Yurdaydın, N., Tekin, N., Gürcan, S. (1996): Değişik ırktan köpeklerde sun'i tohumlama çalışmaları. Lalahan Hay. Arş. ...