- Manaus, Amazonas, Brazil
The phytochemical study of Macrolobium acaciifolium extracts allowed the isolation and identification of 18 substances, 15 of which are reported for the first time in this species. From the methanolic extract of the leaves, the flavonoids... more
The phytochemical study of Macrolobium acaciifolium extracts allowed the isolation and identification of 18 substances, 15 of which are reported for the first time in this species. From the methanolic extract of the leaves, the flavonoids luteolin-3'-O-α-L-rhamnoside (1), neoastilbin (2), astilbin (3), neoisoastilbin (4), isoastilbin (5), quercetin-3-O-α-L-rhamnoside (6), quercetin-3-O-rutinoside (7), kaempferol-3-O-rutinoside (8), quercetin-3-O-glicoside (9) and kaempferol-7-O-rutinoside (10) were isolated. From the methanolic extract of branches, the flavonoids chrysoeriol (13), apigenin (14), 7,4'-dihydroxyflavone (16), eriodictyol (17) and luteolin (18), in addition to the phenolic acids 3,4-dihydroxybenzoic acid (11), 3-methoxy,4-hydroxybenzoate (12) and methyl 3,4-dihydroxybenzoate (15) were isolated. The structural identification of the compounds was established by proton nuclear magnetic resonance (1 H NMR), carbon-13 nuclear magnetic resonance (13 C NMR), heteronuclear single quantum coherence (HSQC), heteronuclear multiple bond correlation (HMBC) and correlated spectroscopy (COSY) analyses. In this paper, we also discuss about the anisotropic effects on H-1'', H-2'', H-5'' and H-6'' rhamnose of isomers dihydroflavonoids.
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Vismia japurensis Reichardt is a plant of ecological and chemical importance from which a variety of bioactive substances have been isolated. The current study aimed to establish in vitro cultures of this species as a source of secondary... more
Vismia japurensis Reichardt is a plant of ecological and chemical importance from which a variety of bioactive substances have been isolated. The current study aimed to establish in vitro cultures of this species as a source of secondary metabolites. Appropriate decontamination treatments and germination tests were performed and, after in vitro culture establishment, the propagated plants were multiplied in a sterile environment to increase the biomass of available experimental material. Seeds showed low contamination and a high germination percentage on Woody Plant Medium (WPM) supplemented with gibberellic acid (both at concentrations of 5 and 10 mg/L). V. japurensis nodal segments rapidly regenerated when first grown in WPM and then transplanted to Murashige and Skoog medium (MS). After 60 days in MS medium, the propagated plants were removed, lyophilized, and extracted with hexane and methanol. The hexane extract was fractionated via open column chromatography, and the substance isolated was purified by high performance liquid chromatography. Structural determination of the isolated substance was carried out using one and two-dimensional nuclear magnetic resonance and mass spectrometry. The isolated substance was identified as 1,8,10-trihydroxy-3,10-dimethyl-9(10H)-anthracenone, which, based on the conducted literature search, is reported for the first time.
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Research Interests: Humanities and Fabaceae
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The plant species Connarus favosus is used in folk medicine in the west of Pará state, Brazil, to treat snakebites. To investigate the potential of the aqueous extract of Connarus favosus (AECf) to inhibit hemorrhagic and phospholipase A2... more
The plant species Connarus favosus is used in folk medicine in the west of Pará state, Brazil, to treat snakebites. To investigate the potential of the aqueous extract of Connarus favosus (AECf) to inhibit hemorrhagic and phospholipase A2 activities induced by Bothrops atrox venom (BaV) and to determine the antioxidant and antimicrobial potentials of the extract. AECf was analyzed phytochemically for phenolics (condensed tannins and hydrolyzable tannins) by colorimetry. Antioxidant activity was evaluated by quantitative assays using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Fe(3+)/phenanthroline. Antimicrobial activity was evaluated by the minimal inhibitory concentration test, and cytotoxicity was evaluated using human fibroblast cells (MRC-5). Inhibition of BaV-induced hemorrhagic activity was assessed after oral administration of the extract using pre-treatment, post-treatment and combined (BA plus AECf) treatment protocols. Inhibition of indirect hemolysis caused by phospholipase A2 (PLA2) was investigated in vitro. Interaction between AECf and BaV was investigated by SDS-PAGE electrophoresis, Western blot (Wb) and zymography. The phytochemical profile of AECf revealed ten secondary metabolite classes, and colorimetry showed high total phenolic and total (condensed and hydrolyzable) tannin content. AECf exhibited high antioxidant and antimicrobial potentials. The IC50 for the cytotoxic effect was 51.91 (46.86-57.50) µg/mL. Inhibition of BaV-induced hemorrhagic activity was significant in all the protocols, and inhibition of PLA2 activity was significant with the two highest concentrations. The BaV/AECf mixture produced the same bands as BaV by itself in SDS-PAGE and Wb although the bands were much fainter. Zymography confirmed the proteolytic activity of BaV, but when the venom was pre-incubated with AECf this activity was blocked. AECf was effective in reducing BaV-induced hemorrhagic activity when administered by the same route as that used in folk medicine and exhibited antioxidant and antimicrobial potentials.
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O aumento da competitividade e da produtividade impacta na elaboração e na ação de políticas públicas que potencializam a importância dos ecossistemas frente ao desenvolvimento tecnológico. Neste contexto, enfatiza-se a definição e o... more
O aumento da competitividade e da produtividade impacta na elaboração e na ação de políticas públicas que potencializam a importância dos ecossistemas frente ao desenvolvimento tecnológico. Neste contexto, enfatiza-se a definição e o papel da Biotecnologia como um processo tecnológico que permite a utilização de material biológico para fins científicos, tecnológicos e industriais. Nesta perspectiva, os objetivos deste trabalho, são: demonstrar a interação da gestão da inovação da Biotecnologia com a cadeia produtiva e analisar a cadeia produtiva do camu-camu (Myrciaria dubia H. B. K.) no Amazonas. A pesquisa foi desenvolvida com trabalhos de campo e levantamento de dados oficiais (IBGE, IPEA, entre outros) para identificar temas e critérios a serem utilizados na análise espacial da cadeia produtiva de produtos oriundos do camu-camu, no período de 2014 a 2018. O camu-camu está entre os frutos com o real potencial funcional e industrial, devido aos elevados teores de vitamina C. A rel...
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Este estudo analisa o camu-camu (myrciaria dubia; myrtaceae) como elemento da biodiversidade e recurso territorial mediado pela biotecnologia. Considerando as dimensões comerciais que o fruto assume no estado do Amazonas, o objetivo é... more
Este estudo analisa o camu-camu (myrciaria dubia; myrtaceae) como elemento da biodiversidade e recurso territorial mediado pela biotecnologia. Considerando as dimensões comerciais que o fruto assume no estado do Amazonas, o objetivo é analisar a biodiversidade vegetal como um recurso territorial. A investigação foi realizada no período de 2014 a 2018 e contou com o levantamento bibliográfico sobre o camu-camu, assim como realização de entrevistas e trabalho de campo nos municípios de Manaus e Silves- Am. O camu-camu, como elemento da biodiversidade vegetal, é natural, mas a sua utilização e constituição, como recurso territorial e componente de cadeias produtivas, é socialmente produzida com maior ou menor agregação da biotecnologia, de técnicas ou investimentos de capital. O objetivo deste trabalho foi abordar a Biodiversidade vegetal como um recurso, por meio, da análise do fruto camu-camu (myrciaria dubia; myrtaceae), característico da Biodiversidade vegetal amazônica. Para a com...
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Artocarpus heterophyllus is an invasive exotic species known for its negative impacts on Brazilian ecosystems. The aim of this study was to evaluate the distribution and population structure of A. heterophyllus in the plant community of a... more
Artocarpus heterophyllus is an invasive exotic species known for its negative impacts on Brazilian ecosystems. The aim of this study was to evaluate the distribution and population structure of A. heterophyllus in the plant community of a forest fragment in the Amazon. Individuals of this species were subject to active search at Sumaúma State Park, in Manaus, and two plots were delimited, where these individuals were counted. In each plot, the jackfruit tree individual with the largest Diameter at Breast Height (DBH) was centralized, and from it, native species were inventoried. The parameters of relative density, relative dominance and the importance value index were estimated. Jackfruit tree individuals in the plots had grouped distribution and their number in plots 1 and 2 was 308 and 872 individuals, respectively. In quadrant 1, 254 jackfruit trees and 118 native individuals were recorded, and in quadrant 2, 629 jackfruit and 130 native individuals. The jackfruit tree reached in...
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Guarana () is largely consumed in Brazil in high energy drinks and dietary supplements because of its stimulant activity on the central nervous system. Although previous studies have indicated that guarana has some protective effects in... more
Guarana () is largely consumed in Brazil in high energy drinks and dietary supplements because of its stimulant activity on the central nervous system. Although previous studies have indicated that guarana has some protective effects in Parkinson's (PD), Alzheimer's (AD), and Huntington's (HD) disease models, the underlying mechanisms are unknown. Here, we investigated the protective effects of guarana hydroalcoholic extract (GHE) in models of HD and AD. GHE reduced polyglutamine (polyQ) protein aggregation in the muscle and also reduced polyQ-mediated neuronal death in ASH sensory neurons and delayed -amyloid-induced paralysis in a caffeine-independent manner. Moreover, GHE's protective effects were not mediated by caloric restriction, antimicrobial effects, or development and reproduction impairment. Inactivation of the transcription factors SKN-1 and DAF-16 by RNAi partially blocked the protective effects of GHE treatment in the AD model. We show that the protecti...
Research Interests: Caenorhabditis elegans, Metabolism, Biology, Medicine, Antioxidants, and 15 moreCaffeine, Animal, Animals, Antioxidant, Lifespan, Antioxidant Activity, Drug effect, Bacterial growth, Controlled Study, Huntington disease, Glutathione Transferase, Alzheimer Disease, Catechin, Epicatechin, and autophagosome
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The Amazon forest is rich in plant species diversity, among them,Piranhea trifoliata stands out, which is popularly known as piranheira, because their fruits are eaten by fish. Their barks are used as bath composition on uterus... more
The Amazon forest is rich in plant species diversity, among them,Piranhea trifoliata stands out, which is popularly known as piranheira, because their fruits are eaten by fish. Their barks are used as bath composition on uterus inflammation and as tea in malaria treatment. This study aimed to fractionate the dichloromethane and dichloromethane phase from methanolic extract of leaves of Piranhea trifoliata. The leaves were dried, grounded and extracted with dichloromethane, methanol and water. The methanol extract was partitioned with dichloromethane and ethyl acetate. The chromatographic fractionation yielded six pentacyclic triterpenoids: friedelan-3-one, 28-hydroxy-friedelan-3-one, 30-hydroxy-friedelan-3-one, lupeol, α- and β-amyrin mixture, besides the mixture of the steroids: β-sitosterol and stigmasterol. The substances structures were identified by 1H- and13C-Nuclear Magnetic Resonance (NMR) analysis and literature data comparison. This is the first report describing the chemi...
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The endophytic fungal community of the Amazonian medicinal plant Arrabidaea chica (Bignoniaceae) was evaluated based on the hypothesis that microbial communities associated with plant species in the Amazon region may produce metabolites... more
The endophytic fungal community of the Amazonian medicinal plant Arrabidaea chica (Bignoniaceae) was evaluated based on the hypothesis that microbial communities associated with plant species in the Amazon region may produce metabolites with interesting bioactive properties. Therefore, the antimicrobial and antioxidant activities of the fungal extracts were investigated. A total of 107 endophytic fungi were grown in liquid medium and the metabolites were extracted with ethyl acetate. In the screening of fungal extracts for antimicrobial activity, the fungus identified as Botryosphaeria mamane CF2-13 was the most promising, with activity against E. coli, S. epidermidis, P. mirabilis, B. subtilis, S. marcescens, K. pneumoniae, S. enterica, A. brasiliensis, C. albicans, C. tropicalis and, especially, against S. aureus and C. parapsilosis (MIC = 0.312 mg/mL). Screening for antioxidant potential using the DPPH elimination assay showed that the Colletotrichum sp. CG1-7 endophyte extract exhibited potential activity with an EC50 of 11 ug/mL, which is equivalent to quercetin (8 ug/mL). The FRAP method confirmed the antioxidant potential of the fungal extracts. The presence of phenolic compounds and flavonoids in the active extracts was confirmed using TLC. These results indicate that two of the fungi isolated from A. chica exhibit significant antimicrobial and antioxidant potential.
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Os micro-organismos endofíticos apresentam um enorme potencial para a produção de substâncias bioativas. A planta Duroia macrophylla produz alcaloides com atividade antitumoral e antimicobacteriana, sendo selecionada para a pesquisa do... more
Os micro-organismos endofíticos apresentam um enorme potencial para a produção de substâncias bioativas. A planta Duroia macrophylla produz alcaloides com atividade antitumoral e antimicobacteriana, sendo selecionada para a pesquisa do potencial biotecnológico dos seus micro-organismos endofíticos. As folhas de D. macrophylla foram lavadas, submetidas à desinfecção, fragmentadas e inoculadas em BDA e Ágar Sabouraud com oxitetraciclina e incubadas a 30 °C por 20 dias. Isolaram-se 47 fungos e destes, 21 fungos morfologicamente diferentes foram selecionados para a prospecção química e biológica. O cultivo submerso foi realizado em Caldo Sabouraud ou Batata Dextrose, a 26 °C e 120 rpm durante 14-30 dias. Os líquidos metabólicos foram filtrados e submetidos à partição líquido-líquido com diclorometano e acetato de etila. Os metabólitos do micélio foram extraídos em ultrassom com diclorometano, acetato de etila e metanol. Os extratos e fases foram analisados por cromatografia em camada delgada e por ressonância magnética nuclear de 1H. Além disso, eles foram testados quanto às atividades antimicrobiana, antioxidante e contra Artemia salina. Como resultado deste trabalho a taxa de colonização fúngica de D. macrophylla foi de 94%. Nos 21 extratos foram encontrados indícios de substâncias fenólicas, terpenos, açúcares e em quatro deles, alcaloides. As fases AcOEt dos fungos Dm SB 43 e Dm BDA 12c foram ativas contra A. salina, com CL50 de 109,5 e 605,5 μg/mL, respectivamente. Nenhum extrato apresentou atividade antioxidante e 18 mostraram atividade antimicrobiana pelo menos para uma bactéria. O fungo Dm SB 33 apresentou o maior espectro de atividade, sendo ativo contra quatro bactérias: Enterobacter cloacae, Staphylococcus aureus, Acinetobacter baumanii e Pseudomonas aeruginosa. Apenas o fungo Dm SB 33 demonstrou atividade contra S. aureus e não foram observadas atividades contra E. coli. Portanto, este estudo demonstrou a diversidade de classes químicas e o potencial de atividades biológicas dos fungos endofíticos de D. macrophylla.
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Research Interests: Chemistry, Artemia, Cytotoxicity, Flavonoids, Animal, and 15 moreAnimals, Antioxidant, Chemical Analysis, Chalcones, Antibacterial activity, Antioxidant Activity, Artemia salina, Fabaceae, Fractionation, Controlled Study, Antiinfective Agent, Flavonoid, Biological activity, Flavones, and Chemical Structure
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Research Interests: Marine Biology, Biology, Brazil, Ecology, Biodiversity, and 15 moreMedicine, Biological Sciences, Anthozoa, Animals, Bacteria, Marine invertebrates, CHEMICAL SCIENCES, Isolation, Marine Fungi, Biological activity, Active Ingredient, Molecular Structure, Marine Natural Product, Medical and Health Sciences, and Biological products
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The antimicrobial potential of Aspergillus sp., isolated from the Amazon biome, which is stored at the Amazon Fungi Collection-CFAM at ILMD/FIOCRUZ, was evaluated. The fungal culture was cultivated in yeast extract agar and sucrose (YES)... more
The antimicrobial potential of Aspergillus sp., isolated from the Amazon biome, which is stored at the Amazon Fungi Collection-CFAM at ILMD/FIOCRUZ, was evaluated. The fungal culture was cultivated in yeast extract agar and sucrose (YES) for cold extraction of the biocompounds in ethyl acetate at 28 °C for 7 days in a BOD type incubator. The obtained extract was evaluated for its antimicrobial activity against Candida albicans and Gram-positive and negative bacteria by the “cup plate” method and the determination of the minimum inhibitory concentration (MIC) by the broth microdilution method. The extract was subjected to thin layer chromatography (TLC) and fractionated by open and semipreparative column chromatography. The fractions of interest had their chemical constituents elucidated by nuclear magnetic resonance and mass spectrometry. The elucidated molecule was evaluated for cytotoxicity against the human fibroblast strain (MRC5). The extract presented inhibitory activity again...
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Non-tuberculous mycobacteria are environmental species of increasing clinical importance in the last years and have been reported as etiologic agent in various types of infections. Generally, they have great intrinsic resistance to... more
Non-tuberculous mycobacteria are environmental species of increasing clinical importance in the last years and have been reported as etiologic agent in various types of infections. Generally, they have great intrinsic resistance to ordinary antimicrobials conventional, which motivates to search new pharmacological alternatives, such as those obtained from natural sources. This study evaluated the activity of classic anti-tuberculosis drugs such as streptomycin (SMR), ethambutol (EMB), isoniazid (INH), rifampin (RIF) and crude extracts of plant from the Atlantic Forest (Brazil) against Mycobacterium fortuitum and Mycobacterium malmoense . It was observed the minimum inhibitory concentration (MIC) for M. fortuitum: EMB = 16μg/mL; SMR = 4μg/mL; INH and RIF 1μg/mL; crude extract Plathymenia foliolosa = 200μg/mL. For M. malmoense, MIC were: SMR = 4μg/mL; INH = 1μg/mL and RIF = 0.25μg/mL. EMB did not inhibit bacterial growth of this species. Active crude extracts against M. malmoense were...
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Riverine communities in the Brazilian Amazon region use a variety of plants to treat snakebites. These plants can be effective against secondary infections, one of the main complications of snakebites. The aim of this study was to... more
Riverine communities in the Brazilian Amazon region use a variety of plants to treat snakebites. These plants can be effective against secondary infections, one of the main complications of snakebites. The aim of this study was to determine whether plants traditionally used to treat snakebites in the Brazilian Amazon may also have antimicrobial and antioxidant activities, and if so, which classes of chemicals may be responsible for these activities. Aqueous extracts of nine plants were tested in microdilution assays and the more active were prepared using solvents (hexane, methanol and water) and decoction, and nine assays were performed. Assays to determine the antioxidant activity of the most active species were carried out, as well as phytochemistry studies to determine the active components of this species. Bellucia dichotoma exhibited the greatest antimicrobial potential, particularly the hexane, methanol and decoction extracts. In comparative TLC, extracts of this species show...
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The perennial rhizomatous grass Arundo donax L. (Poaceae), the giant reed, is an exotic invasive species in several countries of Europe that is rapidly spreading in the savannas of Central Brazil, locally known as Cerrado. Allelopathy... more
The perennial rhizomatous grass Arundo donax L. (Poaceae), the giant reed, is an exotic invasive species in several countries of Europe that is rapidly spreading in the savannas of Central Brazil, locally known as Cerrado. Allelopathy could facilitate the successful invasion of this species by hampering or suppressing the regeneration of the native vegetation. However, information on the phytotoxicity of A. donax extracts is limited. We investigated the allelopathic potential of A. donax leaf and rhizome extracts, screened them for phytochemicals by thin-layer chromatography (TLC) and nuclear magnetic resonance (1H-NMR), and tested the extracts for antioxidant activity, antimicrobial activity, and cytotoxicity against Artemia salina. Aqueous and methanolic extracts were initially tested in germination and seedling growth bioassays using Lactuca sativa L. (Asteraceae). The aqueous extracts were then tested on five Cerrado tree species and on Megathyrsus maximus, an invasive, alien g...
Research Interests: Botany, Biology, Allelopathy, Ulva lactuca, Germination, and 3 moreSeedling, Phytotoxicity, and Rhizome
Os micro-organismos endofíticos apresentam um enorme potencial para a produção de substâncias bioativas. A planta Duroia macrophylla produz alcaloides com atividade antitumoral e antimicobacteriana, sendo selecionada para a pesquisa do... more
Os micro-organismos endofíticos apresentam um enorme potencial para a produção de substâncias bioativas. A planta Duroia macrophylla produz alcaloides com atividade antitumoral e antimicobacteriana, sendo selecionada para a pesquisa do potencial biotecnológico dos seus micro-organismos endofíticos. As folhas de D. macrophylla foram lavadas, submetidas à desinfecção, fragmentadas e inoculadas em BDA e Ágar Sabouraud com oxitetraciclina e incubadas a 30 °C por 20 dias. Isolaram-se 47 fungos e destes, 21 fungos morfologicamente diferentes foram selecionados para a prospecção química e biológica. O cultivo submerso foi realizado em Caldo Sabouraud ou Batata Dextrose, a 26 °C e 120 rpm durante 14-30 dias. Os líquidos metabólicos foram filtrados e submetidos à partição líquido-líquido com diclorometano e acetato de etila. Os metabólitos do micélio foram extraídos em ultrassom com diclorometano, acetato de etila e metanol. Os extratos e fases foram analisados por cromatografia em camada delgada e por ressonância magnética nuclear de 1H. Além disso, eles foram testados quanto às atividades antimicrobiana, antioxidante e contra Artemia salina. Como resultado deste trabalho a taxa de colonização fúngica de D. macrophylla foi de 94%. Nos 21 extratos foram encontrados indícios de substâncias fenólicas, terpenos, açúcares e em quatro deles, alcaloides. As fases AcOEt dos fungos Dm SB 43 e Dm BDA 12c foram ativas contra A. salina, com CL50 de 109,5 e 605,5 μg/mL, respectivamente. Nenhum extrato apresentou atividade antioxidante e 18 mostraram atividade antimicrobiana pelo menos para uma bactéria. O fungo Dm SB 33 apresentou o maior espectro de atividade, sendo ativo contra quatro bactérias: Enterobacter cloacae, Staphylococcus aureus, Acinetobacter baumanii e Pseudomonas aeruginosa. Apenas o fungo Dm SB 33 demonstrou atividade contra S. aureus e não foram observadas atividades contra E. coli. Portanto, este estudo demonstrou a diversidade de classes químicas e o potencial de atividades biológicas dos fungos endofíticos de D. macrophylla.
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Os micro-organismos endofíticos apresentam um enorme potencial para a produção de substâncias bioativas. A planta Duroia macrophylla produz alcaloides com atividade antitumoral e antimicobacteriana, sendo selecionada para a pesquisa do... more
Os micro-organismos endofíticos apresentam um enorme potencial para a produção de substâncias bioativas. A planta Duroia macrophylla produz alcaloides com atividade antitumoral e antimicobacteriana,
sendo selecionada para a pesquisa do potencial biotecnológico dos seus micro-organismos endofíticos. As folhas de D. macrophylla
foram lavadas, submetidas à desinfecção, fragmentadas e inoculadas em BDA e Ágar Sabouraud com oxitetraciclina e incubadas a 30 °C por 20 dias. Isolaram-se 47 fungos e destes, 21 fungos morfologicamente diferentes foram selecionados para a prospecção química e biológica. O cultivo submerso foi realizado em Caldo Sabouraud ou Batata Dextrose, a 26 °C e 120 rpm durante 14-30 dias. Os líquidos metabólicos foram filtrados e submetidos à partição líquido-líquido com diclorometano e
acetato de etila. Os metabólitos do micélio foram extraídos em ultrassom com diclorometano, acetato de etila e metanol. Os extratos e fases foram analisados por cromatografia em camada delgada e por ressonância magnética nuclear de 1H. Além disso, eles foram testados quanto às atividades antimicrobiana, antioxidante e contra Artemia salina. Como resultado deste trabalho a taxa de colonização fúngica de D. macrophylla foi de 94%. Nos 21 extratos foram encontrados
indícios de substâncias fenólicas, terpenos, açúcares e em quatro deles, alcaloides. As fases AcOEt dos fungos Dm SB 43 e Dm BDA 12c foram ativas contra A. salina, com CL50 de 109,5 e 605,5 μg/mL, respectivamente. Nenhum extrato apresentou atividade antioxidante e
18 mostraram atividade antimicrobiana pelo menos para uma bactéria. O fungo Dm SB 33 apresentou o maior espectro de atividade, sendo
ativo contra quatro bactérias: Enterobacter cloacae, Staphylococcus aureus, Acinetobacter baumanii e Pseudomonas aeruginosa. Apenas
o fungo Dm SB 33 demonstrou atividade contra S. aureus e não foram observadas atividades contra E. coli. Portanto, este estudo demonstrou a diversidade de classes químicas e o potencial de atividades biológicas dos fungos endofíticos de D. macrophylla.
sendo selecionada para a pesquisa do potencial biotecnológico dos seus micro-organismos endofíticos. As folhas de D. macrophylla
foram lavadas, submetidas à desinfecção, fragmentadas e inoculadas em BDA e Ágar Sabouraud com oxitetraciclina e incubadas a 30 °C por 20 dias. Isolaram-se 47 fungos e destes, 21 fungos morfologicamente diferentes foram selecionados para a prospecção química e biológica. O cultivo submerso foi realizado em Caldo Sabouraud ou Batata Dextrose, a 26 °C e 120 rpm durante 14-30 dias. Os líquidos metabólicos foram filtrados e submetidos à partição líquido-líquido com diclorometano e
acetato de etila. Os metabólitos do micélio foram extraídos em ultrassom com diclorometano, acetato de etila e metanol. Os extratos e fases foram analisados por cromatografia em camada delgada e por ressonância magnética nuclear de 1H. Além disso, eles foram testados quanto às atividades antimicrobiana, antioxidante e contra Artemia salina. Como resultado deste trabalho a taxa de colonização fúngica de D. macrophylla foi de 94%. Nos 21 extratos foram encontrados
indícios de substâncias fenólicas, terpenos, açúcares e em quatro deles, alcaloides. As fases AcOEt dos fungos Dm SB 43 e Dm BDA 12c foram ativas contra A. salina, com CL50 de 109,5 e 605,5 μg/mL, respectivamente. Nenhum extrato apresentou atividade antioxidante e
18 mostraram atividade antimicrobiana pelo menos para uma bactéria. O fungo Dm SB 33 apresentou o maior espectro de atividade, sendo
ativo contra quatro bactérias: Enterobacter cloacae, Staphylococcus aureus, Acinetobacter baumanii e Pseudomonas aeruginosa. Apenas
o fungo Dm SB 33 demonstrou atividade contra S. aureus e não foram observadas atividades contra E. coli. Portanto, este estudo demonstrou a diversidade de classes químicas e o potencial de atividades biológicas dos fungos endofíticos de D. macrophylla.