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Zeolites are natural or synthetic crystalline aluminosilicates with ion exchanging properties. Although zeolites have been used as animal feed additive for more than 40 years, there is no report about the effects of zeolites on... more
Zeolites are natural or synthetic crystalline aluminosilicates with ion exchanging properties. Although zeolites have been used as animal feed additive for more than 40 years, there is no report about the effects of zeolites on spermatological traits. The aim of this study was to designate the effects of zeolite on the testicular weight, live body weight and spermatological characteristics in rats. Twenty-four, 8- week-old, Sprague-Dawley male rats were used in the study. Rats were weighed and randomly divided into control and feeding groups at the beginning of the study. Feeding groups were formed by adding zeolite at rates of 2%, 4% and 6% to standard rat food. Subsequently the prepared rations were pelleted in 1 cm diameter and fed to the rats for 8 weeks. At the end of the study live weights of all rats were recorded and all animals were anesthetized with sodium thiopental. Then, the testes, epididymis, seminal vesicles and ventral prostate were removed, cleared from the surroun...
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The aim of the study was to determine whether a dose (0.6 µg/kg/d) quite lower than the prolactin-lowering dose of cabergoline, prepared for humans, would be a safe and effective method for the stimulation of oestrus in bitches at... more
The aim of the study was to determine whether a dose (0.6 µg/kg/d) quite lower than the prolactin-lowering dose of cabergoline, prepared for humans, would be a safe and effective method for the stimulation of oestrus in bitches at secondary anoestrus or late anoestrus. Twenty-four pure blood bitches from various breeds were used in the study at their already determined periods of anoestrus. The treatment group included bitches at late and prolonged anoestrus. Eight bitches that had not shown any signs of oestrus for the preceding 370 to 485 d formed the secondary anoestrus group. Eight of the 16 bitches at late anoestrus (days 165-280) have accomplished the late anoestrus group and another 8 have been chosen randomly for the control group (untreated). Cabergoline was orally administrated until day 2 after the onset of pro-oestrus or for a maximum of 42 d. Blood samples were taken daily from each bitch during the first 5 d of behavioural oestrus to measure progesterone concentrations. In the secondary anoestrus and late anoestrus groups, oestrus was induced on days 4-14 and 12-45 at a ratio of 75.0% (6/8) and 87.5% (7/8), respectively.. The mean pro-oestrus and behavioural oestrus durations, serum progesterone concentrations on day 5 of oestrus, ovulation rates, pregnancy rates, and the mean litter sizes in secondary anoestrus, late anoestrus, and control groups were found to be similar. None of the dogs had any adverse gastrointestinal effects associated with cabergoline administration. The results of the present study suggest that the administration of 0.6 µg/kg/d of cabergoline is a safe and effective treatment for secondary anoestrus in bitches.
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The present study evaluated follicular superstimulatory (FSS) and superovulatory (SOV) responses and in vivo embryo production in lactating Simmental cows treated with FSH starting 1 or 2 days after follicle aspiration (FA). The... more
The present study evaluated follicular superstimulatory (FSS) and superovulatory (SOV) responses and in vivo embryo production in lactating Simmental cows treated with FSH starting 1 or 2 days after follicle aspiration (FA). The performance of a lengthened superovulation program, named 6dFSH-P36-hCG60, is described. At random stages of the estrous cycle, cows (n = 52) were subjected to ultrasound-guided transvaginal aspiration of all follicles ≥ 5 mm. After FA, cows were randomly assigned to one of two groups in which FSH treatments started 1 or 2 days after FA (groups FA-1D and FA-2D, respectively). Cows were superstimulated with a total of 500 μg pFSH over 6 days on a decreasing dose schedule and were pre-treated with a single dose of 400 IU of eCG 24 h before the start of FSH treatments. Follicular superstimulatory (the mean numbers of follicles ≥ 8 mm on the day of hCG treatment) and SOV responses (the mean numbers of CL and cows with ≥ 3 CL at the time of collection) were similar in FA-1D and FA-2D groups. However, when compared to FA-1D group, the number of unfertilized ova tended to decrease (0.4 vs 1.7; P = 0.065) and percentage of fertilized ova tended to increase (95.8% vs 84.6%; P = 0.066) in FA-2D group. Moreover, the mean numbers and percentages of both transferable embryos (8.0 and 77.6% vs 6.4 and 57.7%) and freezable embryos (5.3 and 51.5% vs 3.5 and 31.1%) were numerically higher in FA-2D group than FA-1D group. The results of the study suggest that starting a lengthened superovulation programs in Simmental cows 2 days after FA has potential to increase percentage of fertilized ova and the number of transferable and freezable embryos, although new studies may be needed to confirm this findings.
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Research Interests: Endocrinology, Medicine, Internal Medicine, Spermatogenesis, Nitric oxide, and 15 moreAnimals, Male, Semen Analysis, Clinical Sciences, Phosphodiesterase Inhibitors, Rats, Spermatozoon, Semen, Spermatozoa, Epididymis, Isoniazid, Luteinizing Hormone, Semen Quality, Follicle stimulating hormone, and purines
This is the very first report that suggests high pregnancy rates can be obtained with use of the Doublesynch protocol in anestrous dairy cows. Recently, a new synchronization method has been developed (Doublesynch) that resulted in... more
This is the very first report that suggests high pregnancy rates can be obtained with use of the Doublesynch protocol in anestrous dairy cows. Recently, a new synchronization method has been developed (Doublesynch) that resulted in synchronized ovulations both after the first and second gonadotropin-releasing hormone (GnRH) treatments. It was suggested that this protocol has the potential to increase the pregnancy rates in primiparous dairy cows. The aim of the current study was to confirm the success of the Doublesynch protocol and further to investigate the effect of this method on pregnancy rates in anestrous cows. Lactating primiparous Holstein (Bos taurus) cows (n=165) between 60 and 172 d postpartum were monitored twice with 10-d intervals (on Days -10 and 0) by ultrasonography, and blood samples were collected. Cows were classified as anestrous if both blood samples had progesterone (P4) concentration <1 ng/mL and as cyclic if at least one of the two samples had P4 concentration >or=1 ng/mL. Cyclic cows were classified again as cyclic-high P4 (having an active corpus luteum) if the second blood samples had P4 concentrations >or=1 ng/mL and as cyclic-low P4 if P4 concentrations were <1 ng/mL on Day 0. Then, the cows classified as anestrous (n=51), cyclic-high P4 (n=63), or cyclic-low P4 (n=51) were put into two treatment groups (Ovsynch or Doublesynch) randomly to establish six groups. Cows in the Ovsynch group were administered a GnRH (lecirelin 50 microg, im) on Day 0, PGF (Prostaglandin F2 alpha, D-cloprostenol 0.150 mg, im) on Day 7, and a second dose of GnRH 48 h later. Cows in the Doublesynch group were administered a PGF on Day 0, GnRH on Day 2, a second PGF on Day 9, and a second GnRH on Day 11. Timed artificial insemination (TAI) was performed 16 to 20 h after the second GnRH in both treatment groups. Pregnancy diagnosis was conducted (by ultrasonography) 45+/-5 d after TAI. In anestrous cows and those with high and low progesterone concentration at treatment onset, Doublesynch treatment led to markedly increased pregnancy rates with respect to Ovsynch treatment (P<0.05). On the overall analysis of data, it was revealed that the Doublesynch method increased pregnancy rates by 43 percentage units (29.8% vs. 72.8%, P<0.0001) in relation to Ovsynch. Pregnancy rates of cows having small, medium, or large follicles at the day of second GnRH administration were similar in the Doublesynch group (70.4%, 85.2%, and 63.0%, respectively; P>0.05), whereas pregnancy rates reduced dramatically as follicle size increased in the Ovsynch group, particularly in cows with follicles greater than 16 mm (45.5%, 28.1%, and 5.3%, respectively; P<0.05). Our results confirm and support observations that the Doublesynch protocol increases the pregnancy rates in postpartum primiparous cows as reported previously. Our data also demonstrate that the Doublesynch method increases the pregnancy rates in anestrous cows. Thus, these data suggest that the Doublesynch protocol can be used to obtain satisfactory pregnancy rates after TAI in both anestrous and cycling primiparous dairy cows regardless of stage of estrous cycle.
Research Interests: Animal Science, Medicine, Biological Sciences, Pregnancy, Progesterone, and 15 moreFemale, Animals, Blood sampling, Dairy Cow, Cattle, Artificial insemination, Gonadotropin Releasing Hormone, Bos Taurus, Dairying, Pregnancy Rate, Corpus Luteum, Insemination, Estrous cycle, Estrus Synchronization, and Medical and Health Sciences
In this study, hypoosmotic swelling (HOS), thermal stress (TS) and modified cervical mucus penetration (mCMP) tests have been used with routine tests for the assessment of semen quality. This is the first study in which the comparison of... more
In this study, hypoosmotic swelling (HOS), thermal stress (TS) and modified cervical mucus penetration (mCMP) tests have been used with routine tests for the assessment of semen quality. This is the first study in which the comparison of potential fertility estimation of fore-mention three tests was performed. Bull semen samples were divided into two fertility groups (high: n=3, low: n=3), according to their post-insemination NRR (non-return rate). Prior to the tests, post-thawed spermatological characteristics were assessed after which HOS, TS and mCMP tests were carried out. In the HOS test, the ratio of swollen cells, in the TS test the motility, and in the mCMP test the number of spermatozoa penetrating the cervical mucus, were examined. The relationship between the tests and fertility was also evaluated. HOS test was carried out according to different incubation times and temperatures (37 degrees C 60 min/41 degrees C 15 min/41 degrees C 30 min/46 degrees C 15 min/46 degrees C 30 min). For TS test, samples were subjected to various temperatures for different periods (no incubation (37 degrees C)/41 degrees C 15 min/41 degrees C 30 min/46 degrees C 15 min/46 degrees C 30 min). The mCMP test were subjected to various temperatures for the same period (37 degrees C 15 min/41 degrees C 15 min). In this study, post-thawed motility was found to be similar in high and low fertility groups. However, it has been determined that acrosomal (p<0.01) and other morphological defects (p<0.05) were low in the high fertility group. When HOS test was carried out at 37 degrees C, no difference was observed between the bulls with high and low fertility, but at 41 and 46 degrees C, results of high fertility group were significantly higher than those of low fertility group (p<0.01). Similarly in TS test, the progressive motility rates of high fertility bulls was higher after thermal practices at 41 and 46 degrees C (p<0.01). In mCMP test, at 37 degrees C, the number of cells that had penetrated was similar. However, significant differences were observed in the incubation at 41 degrees C (p<0.01). It has been concluded that for the estimation of potential fertility of bulls, HOS, TS and mCMP tests, in combination with routine spermatological tests can be used and the use of further penetration distance range (PDR2) in mCMP test and higher temperatures such as 41 degrees C instead of 37 degrees C, during the incubations in the afore-mentioned performance tests, is more determinative.
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The purpose of this study is to bridge this gap by investigating effects of long term 900 MHz mobile phone exposure on reproductive organs of male rats. The study was carried out on 14 adult Wistar Albino rats by dividing them randomly... more
The purpose of this study is to bridge this gap by investigating effects of long term 900 MHz mobile phone exposure on reproductive organs of male rats. The study was carried out on 14 adult Wistar Albino rats by dividing them randomly into two groups (n: 7) as sham group and exposure group. Rats were exposed to 900 MHz radiofrequency (RF) radiation emitted from a GSM signal generator. Point, 1 g and 10 g specific absorption rate (SAR) levels of testis and prostate were found as 0.0623 W/kg, 0.0445 W/kg and 0.0373 W/kg, respectively. The rats in the exposure group were subject to RF radiation 3 h per day (7 d a week) for one year. For the sham group, the same procedure was applied, except the generator was turned off. At the end of the study, epididymal sperm concentration, progressive sperm motility, abnormal sperm rate, all-genital organs weights and testis histopathology were evaluated. Any differences were not observed in sperm motility and concentration (p > 0.05). However, the morphologically normal spermatozoa rates were found higher in the exposure group (p < 0.05). Although histological examination showed similarity in the seminiferous tubules diameters in both groups, tunica albuginea thickness and the Johnsen testicular biopsy score were found lower in the exposure group (p < 0.05, p < 0.0001). In conclusion, we claim that long-term exposure of 900 MHz RF radiation alter some reproductive parameters. However, more supporting evidence and research is definitely needed on this topic.
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Research Interests: Endocrinology, Animal Science, Biology, Fertility, Litter, and 4 moreVitamin, Beta Carotene, Carotene, and Estrous cycle
Since their reproduction is seasonal, goat lambs can not mate before the breeding season begins, even if they have reached breeding maturity. This situation leads to economic losses for farmers. Estrus synchronization with progesterone... more
Since their reproduction is seasonal, goat lambs can not mate before the breeding season begins, even if they have reached breeding maturity. This situation leads to economic losses for farmers. Estrus synchronization with progesterone and PMSG combination is widely practiced to produce off-season heat. However, different applications are made regarding the dose and timing of PMSG. In the study, the effects of PMSG (500 IU) administration at two different times on estrus and pregnancy rates and the number of offspring were investigated in estrus-synchronized goat lambs. Twenty three kilis goat lambs 13-15 months old that had never mated before were treated (out of season) with a progesterone sponge (20 mg flugestone acetate) for 11 days and 250 mcg PGF2α two days before sponge removal. While PMSG was applied to lambs in group 1 (n:11, hour -48 group) two days before sponge removal, it was applied to lambs in group 2 (n:12, hour 0 group) on the day of sponge removal. Goat lambs stand...
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The aim of this study was to create new strategies to increase the pregnancy rate in “ovsynch protocol” treatment. Two programs for synchronisation of ovulation and for synchronisation of ovulation and oestrus, similar to the ovsynch,... more
The aim of this study was to create new strategies to increase the pregnancy rate in “ovsynch protocol” treatment. Two programs for synchronisation of ovulation and for synchronisation of ovulation and oestrus, similar to the ovsynch, were developed for the use in lactating primiparous dairy cows. Lactating Holstein-Friesian cows were randomly divided into five treatment groups: the GPG group (ovsynch) was treated with GnRH on day 0, PGF (PGF2α) on day 7, and received the second dose of GnRH 48 h later; the groups -7PGPG and 2PGPG received the same treatment as the GPG group, but were given an additional injection of PGF 7 and 2 d before the start of the GPG treatment; respectively, the PG9PG group received the same treatment as the -2PGPG group, with the modification that the first GnRH injection was given simultaneously with the first PGF on the 2 nd d; the GPEG group received the same treatment as the GPG group, but was injected an additional oestradiol propionate (EP) 24 h after...
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OZET Gunumuzde dondurulmus embriyolarin transferi ile ustun verim ozelliklerine sahip surulerin olusturulmasi, hastaliklarin kontrolu ve genetik materyallerin uzun sure saklanmasi mumkun olabilmektedir. Ancak donmus embriyolarda eritme... more
OZET Gunumuzde dondurulmus embriyolarin transferi ile ustun verim ozelliklerine sahip surulerin olusturulmasi, hastaliklarin kontrolu ve genetik materyallerin uzun sure saklanmasi mumkun olabilmektedir. Ancak donmus embriyolarda eritme sonrasi tranfer edilebilir embriyo eldesi ve gebelik oranlari istenilen duzeyde degildir. Ozellikle embriyolarin dondurulmasi sirasinda dejenerasyonlar olusmaktadir. Dejenerasyonlarin, embriyolarin kriyoprotektif maddelere maruz kalma sureleri ile toksik etkilerinden meydana geldigi dusunulmektedir. Calismada mezbahada kesilen sigirlarin ovaryumlari kullanildi. Aspirasyon yontemi ile elde edilen oositler (1290 adet) TCM-199 da 22-24 saat sureyle % 5 CO 2 , %5 O 2 , %90N 2 gaz atmosferinde 38,8 oC de in vitro olarak olgunlastirildilar. Olgun oositler IVF-TALP medyumunda 18-24 saat sureyle fertilize edildiler. Fertilizasyon sonrasi 48. saatte cleavage %67,05 (865/1290) saptandi. Embriyolar %10 FCS'li SOF medyumunda 7 gun sureyle % 5 CO 2 , %5 O 2 , ...
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Tree experiments were designed to optimize ram semen cryopreservation and to examine the effect of the e xtender B osmolarity, extension time with extender A and B, presence of glycerol in freezing media, cooling rate from 30 EsC to 5 °C,... more
Tree experiments were designed to optimize ram semen cryopreservation and to examine the effect of the e xtender B osmolarity, extension time with extender A and B, presence of glycerol in freezing media, cooling rate from 30 EsC to 5 °C, and dilution rate on post-thaw ram semen characteristics and fertilization. Ram ejaculates of thick consistency with rapid wave motion (>+++) and >70% initial motility were pooled. Pooled semen was diluted with Tris based extender using a two-step dilution method. Semen motility and morphology related parameters were assessed at the following five steps : (1) Fresh, (2) after dilution with extender A, (3) at 5 ° C, (4) after equilibration, and (5) after thawing at 37 ° C for 30 min. The extender osmolarity affected significantly post-thaw semen motility, defected acrosomes (DA) and total morphological defect (TMD), generally. There was a significant interaction between the osmolarity, cooling rates, glycerol addition time and first dilution t...
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Araştırma, Diyarbakır ilinde üreme mevsimi dışında İvesi ırkı 237 koyun üzerinde yürütüldü. Östrus senkronizasyonu yapılan koyunlar suni tohumlama işleminden hemen önce vajina tabanındaki servikal mukus (SM) miktarına göre “yok” (n= 25),... more
Araştırma, Diyarbakır ilinde üreme mevsimi dışında İvesi ırkı 237 koyun üzerinde yürütüldü. Östrus senkronizasyonu yapılan koyunlar suni tohumlama işleminden hemen önce vajina tabanındaki servikal mukus (SM) miktarına göre “yok” (n= 25), “az” (n= 79) veya “bol” (n= 133) olarak sınıflandırıldı. Koyunlar östrustan 12-18 saat sonra intra-servikal yolla 200×106 motil spermatozoa içeren taze-sulandırılmış sperma ile tohumlandı. Gebelik oranları, suni tohumlamadan (ST) 30-40 gün sonra ultrason aracılığı ile belirlendi. Çara durumuna göre “yok”, “az” ve “bol” olarak sınıflandırılan koyunların gebelik oranları sırasıyla %36.4, %50.6 ve %45.1 olarak bulundu (p>0.05). Çalışmamızdan; İvesi koyunlarda ST öncesinde vajinadaki SM miktarının gebelik oranlarını istatistiksel olarak önemli düzeyde etkilemediği sonucu çıkarılmıştır. Ancak SM miktarına göre “yok” ve “az” olarak sınıflandırılan koyunların gebelik oranları arasında kayda değer sayısal farklılığın olması, SM miktarının fertiliteyi etk...
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The aim of this study was to obtain cloned lambs by somatic cell nuclear transfer. In vitro matured oocytes were enucleated and injected with serum-starved cumulus cells. After electrofusion and ionomycin treatments, reconstructed oocytes... more
The aim of this study was to obtain cloned lambs by somatic cell nuclear transfer. In vitro matured oocytes were enucleated and injected with serum-starved cumulus cells. After electrofusion and ionomycin treatments, reconstructed oocytes were activated by 10 m g/ml cycloheximide (CHX) for 5 h or 2 mM 6-dimethylaminopurine (6-DMAP) for 3 h and cultured in vitro in SOF medium at 38.5°C in a humidified atmosphere of 5% CO 2 , 5% O 2 and 90% N 2 . Cleavage rates were 18.1% (26/144) in CHX and 46.5% (140/301) in 6-DMAP groups; 11.4% of embryos developed to the morula stage in the 6-DMAP group. To investigate the developmental capacity of the embryos to become live young, early cleavage stage embryos (n= 69) were transferred to the oviducts of synchronized recipients. Five out of eight ewes (62.5%) were diagnosed as pregnant at Day 18 according to progesterone assays, and two ewes (25.0%) were diagnosed as pregnant after 45 days according to ultrasound examinations. Two healthy lambs wer...
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This is the very first report that suggests high pregnancy rates can be obtained with use of the Doublesynch protocol in anestrous dairy cows. Recently, a new synchronization method has been developed (Doublesynch) that resulted in... more
This is the very first report that suggests high pregnancy rates can be obtained with use of the Doublesynch protocol in anestrous dairy cows. Recently, a new synchronization method has been developed (Doublesynch) that resulted in synchronized ovulations both after the first and second gonadotropin-releasing hormone (GnRH) treatments. It was suggested that this protocol has the potential to increase the pregnancy rates in primiparous dairy cows. The aim of the current study was to confirm the success of the Doublesynch protocol and further to investigate the effect of this method on pregnancy rates in anestrous cows. Lactating primiparous Holstein (Bos taurus) cows (n=165) between 60 and 172 d postpartum were monitored twice with 10-d intervals (on Days -10 and 0) by ultrasonography, and blood samples were collected. Cows were classified as anestrous if both blood samples had progesterone (P4) concentration <1 ng/mL and as cyclic if at least one of the two samples had P4 concentration >or=1 ng/mL. Cyclic cows were classified again as cyclic-high P4 (having an active corpus luteum) if the second blood samples had P4 concentrations >or=1 ng/mL and as cyclic-low P4 if P4 concentrations were <1 ng/mL on Day 0. Then, the cows classified as anestrous (n=51), cyclic-high P4 (n=63), or cyclic-low P4 (n=51) were put into two treatment groups (Ovsynch or Doublesynch) randomly to establish six groups. Cows in the Ovsynch group were administered a GnRH (lecirelin 50 microg, im) on Day 0, PGF (Prostaglandin F2 alpha, D-cloprostenol 0.150 mg, im) on Day 7, and a second dose of GnRH 48 h later. Cows in the Doublesynch group were administered a PGF on Day 0, GnRH on Day 2, a second PGF on Day 9, and a second GnRH on Day 11. Timed artificial insemination (TAI) was performed 16 to 20 h after the second GnRH in both treatment groups. Pregnancy diagnosis was conducted (by ultrasonography) 45+/-5 d after TAI. In anestrous cows and those with high and low progesterone concentration at treatment onset, Doublesynch treatment led to markedly increased pregnancy rates with respect to Ovsynch treatment (P<0.05). On the overall analysis of data, it was revealed that the Doublesynch method increased pregnancy rates by 43 percentage units (29.8% vs. 72.8%, P<0.0001) in relation to Ovsynch. Pregnancy rates of cows having small, medium, or large follicles at the day of second GnRH administration were similar in the Doublesynch group (70.4%, 85.2%, and 63.0%, respectively; P>0.05), whereas pregnancy rates reduced dramatically as follicle size increased in the Ovsynch group, particularly in cows with follicles greater than 16 mm (45.5%, 28.1%, and 5.3%, respectively; P<0.05). Our results confirm and support observations that the Doublesynch protocol increases the pregnancy rates in postpartum primiparous cows as reported previously. Our data also demonstrate that the Doublesynch method increases the pregnancy rates in anestrous cows. Thus, these data suggest that the Doublesynch protocol can be used to obtain satisfactory pregnancy rates after TAI in both anestrous and cycling primiparous dairy cows regardless of stage of estrous cycle.
Research Interests: Animal Science, Medicine, Biological Sciences, Pregnancy, Progesterone, and 15 moreFemale, Animals, Blood sampling, Dairy Cow, Cattle, Artificial insemination, Gonadotropin Releasing Hormone, Bos Taurus, Dairying, Pregnancy Rate, Corpus Luteum, Insemination, Estrous cycle, Estrus Synchronization, and Medical and Health Sciences
Research Interests: Breeding, Animal reproduction, Medicine, Biological Sciences, Dogs, and 15 morePregnancy, Low Dose, Female, Ovulation, Ovulation Induction, Animals, Blood sampling, Breed, Aqueous Solution, Ovulation rate, Control Group, Estrous cycle, Human Chorionic Gonadotropin, Cabergoline, and Medical and Health Sciences
In the present study, two new short estrus synchronization methods have been developed for lactating dairy cows. The study was completed in three consecutive phases. In experiment (Exp) 1, 32 cows, that were not detected in estrus since... more
In the present study, two new short estrus synchronization methods have been developed for lactating dairy cows. The study was completed in three consecutive phases. In experiment (Exp) 1, 32 cows, that were not detected in estrus since calving between the 50th and 84th post-partum days, were treated with PGF2alpha (PGF, d-cloprostenol, 0.150 mg), estradiol propionate (EP, 2mg) and GnRH (lecirelina, 50 microg) at 24h intervals, respectively, and timed artificial insemination (TAI) was performed 48 h after PGF. Different from Exp 1, EP and GnRH were given at 48 and 60 h, respectively after PGF in Exp 2 (n=20), instead of 24 and 48 h. Ovulations were investigated by ultrasound for 7 days starting from the day of PGF treatment, and ovulation rates were compared with the ones obtained in Exp 1. In Exp 3, cows were given the same treatments as Exp 2, but treatments started at certain estrus stages. Cows detected in estrus and with a confirmed ovulation (n=27) after the second PGF given 11 days apart were assigned to three treatment groups. Treatment was initiated at Day 3 (group metestrus, n=9), Day 12 (group diestrus, n=9) and Day 18 (group proestrus, n=9) after ovulation. All cows included in Exp 3 were TAI between 16 and 20 h after GnRH treatment. In Exp 2 and 3, blood samples were obtained once every 2 days, starting from Day 0 to the 10th day after GnRH injection, and once every 4 days between the 10th and the 22nd days after GnRH to examine post-treatment luteal development. During the study, animals exhibiting natural estrus were inseminated and served as controls (n=85). The rate of estrus was found to be significantly higher in cows with an active corpus luteum (CL) at the start of Exp 1 (72.7% vs. 30.0%, P<0.05) and the pregnancy rate tended to be higher than cows without an active CL (40.9% vs. 10.0%, P=0.08). Compared to those in Exp 1, cows in Exp 2 had higher rates of synchronized ovulation (94.1% vs. 59.1%, P=0.013). In Exp 3, estrus (P<0.001) and pregnancy rates (P=0.01) were found to be significantly higher in cows in the proestrus group than in those in the metestrus group. Comparable pregnancy rates were obtained from the first and second inseminations in Exp 1 and 3 with results from those inseminated at natural estrus (P>0.05). It was concluded from the study that the treatment in Exp 1 and 3 could result in comparable pregnancy rates after timed AI of lactating dairy cows at random stages of the estrus cycle relating to those inseminated at natural estrus, but the stage of the estrus cycle can have significant effects on pregnancy rates.
Research Interests: Animal Science, Animal reproduction, Medicine, Biological Sciences, Female, and 15 moreOvulation, Animals, Blood sampling, Dairy Cow, Cattle, Artificial insemination, Gonadotropin Releasing Hormone, Dairying, Estradiol, Ovulation rate, Corpus Luteum, Insemination, Estrous cycle, Estrus Synchronization, and Medical and Health Sciences
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Özet: Koyunlarda somatik ve embriyonik hücre nüklear transfer yöntemlerini laboratuvarımızda başlatabilmek için, iki farklı partenogenetik aktivasyon protokolü denendi. Oositler mezbahada kesilen koyunların ovaryumlarından elde edildi ve... more
Özet: Koyunlarda somatik ve embriyonik hücre nüklear transfer yöntemlerini laboratuvarımızda başlatabilmek için, iki farklı partenogenetik aktivasyon protokolü denendi. Oositler mezbahada kesilen koyunların ovaryumlarından elde edildi ve piruvat, hormonlar (FSH ve LH) ve %10 FCS katkılı medyum 199 içerisinde 24 saat olgunlaştırıldı. Olgunlaştırılan oositlere 80 µsec süreyle 1.25 kV/cm elektrik akımı verildi ve oositlerin tamamı önce 5 dk ionomisin içerisinde, daha sonra ise 30 dk sitokalasin B içerisinde bırakıldı. İki gruba rastgele ayrılan oositler, 1. grupta 6-DMAP'lı medyumda 2 saat, 2. grupta ise sikloheksimid'li medyumda 3 saat inkübe edildikten sonra kültür medyumuna aktarıldı. Grup I ve II'de elde edilen embriyolarda yarıklanma oranları sırasıyla %82.7 ve %81.3 olurken blastosist oranları %2.3 ve %0 olarak bulundu (P>0.05). Sunulan çalışma koyunların klonlanması konusunda önemli aşamalardan birini oluşturan partenogenetik aktivasyon konusunda ülkemizde gerçekl...
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At present, blastocyst rates in embryos obtained from in vitro maturation of oocytes, and their fertilization and culture, is still not at the desired level. One of the most important problems encountered in in vitro culture studies is... more
At present, blastocyst rates in embryos obtained from in vitro maturation of oocytes, and their fertilization and culture, is still not at the desired level. One of the most important problems encountered in in vitro culture studies is seen in the maturation period of oocytes until they reach the fertilizable level. Transport time of the ovaries and, in particular, temperature of the transport medium used are among the factors affecting complete maturation. The aim of this study was to determine the effects of different transport temperatures (4°C, 32°C) of sheep and cattle ovaries on the in vitro maturation of oocytes. Two experimental groups were formed in the study. Sheep and cattle ovaries were put into saline solution at 32°C. The ovaries were transported at the same temperature (Group I) or at 4°C following a 10-min incubation at room temperature (Group II), in 2–4 h to the laboratory (n = 6). For each group, oocytes were collected from ovaries using the dissection method and ...
Research Interests:
The aim of this study is to investigate the effects of the thiolation on the mucoadhesion characteristics of the gelatinized and crosslinked wheat starch-graft-poly(acrylic acid) [(WS-g-PAA)gc] for potential use in drug delivery via... more
The aim of this study is to investigate the effects of the thiolation on the mucoadhesion characteristics of the gelatinized and crosslinked wheat starch-graft-poly(acrylic acid) [(WS-g-PAA)gc] for potential use in drug delivery via vaginal route. Thiolation of (WS-g-PAA)gc was first time realized using l-cysteine hydrochloride monohydrate (CyS) and thioglycolic acid (TGA). These conjugates [(WS-g-PAA)gcth] were characterized using FTIR. The free SH group, mucoadhesion, cytotoxicity characteristics and the mechanism of the thiolation were also evaluated. To obtain fundamental data for possible application such as drug carrier, in vitro and in vivo progesterone release profiles from the mucoadhesive tablet formulations were also determined. The results showed that, vaginal tablet containing (WS-g-PAA)gc-TGA, which has not contain free SH groups in its structure, displays higher mucoadhesion than (WS-g-PAA)gc and (WS-g-PAA)gc-CyS. This tablet formulation can also be used as a drug carrier in vaginal applications.