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This study aimed to evaluate the performance characteristics of a rapid antigen test developed to detect SARS-CoV-2 (COVID-19), influenza A virus (IAV), and influenza B virus (IBV) (flu) compared with those of the real-time reverse... more
This study aimed to evaluate the performance characteristics of a rapid antigen test developed to detect SARS-CoV-2 (COVID-19), influenza A virus (IAV), and influenza B virus (IBV) (flu) compared with those of the real-time reverse transcription-polymerase chain reaction (rRT-PCR) method. One hundred SARS-CoV-2, one hundred IAV, and twenty-four IBV patients whose diagnoses were confirmed by clinical and laboratory methods were included in the patient group. Seventy-six patients, who were negative for all respiratory tract viruses, were included as the control group. The Panbio™ COVID-19/Flu A&B Rapid Panel test kit was used in the assays. The sensitivity values of the kit were 97.5%, 97.9%, and 33.33% for SARS-CoV-2, IAV, and IBV, respectively, in samples with a viral load below 20 Ct values. The sensitivity values of the kit were 16.7%, 36.5%, and 11.11% for SARS-CoV-2, IAV, and IBV, respectively, in samples with a viral load above 20 Ct. The kit’s specificity was 100%. In conclusi...
Research Interests: Virology, Medicine, Virus, Diagnostics, influenza A virus, and 3 moreCoronavirus, Antigen, and Viral Load
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BACKGROUND The debridement of necrotic and infected tissues, which prolong the wound healing process, is important for the preparation of the wound bed. Therefore, wound-bed preparation and debridement are vital components of venous leg... more
BACKGROUND The debridement of necrotic and infected tissues, which prolong the wound healing process, is important for the preparation of the wound bed. Therefore, wound-bed preparation and debridement are vital components of venous leg ulcer management. We aimed to present a perspective to evaluate the clinical and microbiological efficacy of Maggot Debridement Therapy (MDT) in the treatment of chronic leg ulcers caused by venous insufficiency. METHODS Thirty-eight patients with chronic venous leg ulcers who were referred to our unit with an MDT request were included in the study. Lucilia sericata larvae were applied to the wounds two days a week until the necrotic tissue was cleared. Swab samples were regularly taken before and immediately after each larval application for wound culture. Changes in the percentage of wound surface area and growing pathogenic microorganisms were recorded during the follow-up period. RESULTS The sample consisted of 38 patients with 55 venous leg ulcers. The mean initial ulcer surface area was 99.1 cm2 (range 3 - 500). Complete debridement was achieved in all ulcers in the 2nd week, on average. Twenty-five ulcers (45.5%) were completely debrided with two one-week MDT sessions. Complete wound healing occurred in 42 ulcers (76.4%) after an average of seven MDT sessions. Microorganisms isolated from the wounds significantly decreased immediately after the first MDT session. CONCLUSIONS Although many methods are used in the treatment of venous leg ulcers, they are often not effective. MDT, which is coming into widespread use today, is a simple and effective method in the treatment of these ulcers. Its effects such as biodebridement, disinfection, and growth stimulation can encourage the rapid healing of chronic venous leg ulcers.
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Intestinal parasitic infections are among important health problems in developing countries. In societies living in low socioeconomic conditions, it has been neglected and mostly affects children. It is important to determine the... more
Intestinal parasitic infections are among important health problems in developing countries. In societies living in low socioeconomic conditions, it has been neglected and mostly affects children. It is important to determine the prevalence and type of intestinal parasites in order to determine the intervention strategies for these infections. Therefore, the aim of this study is to evaluate intestinal parasite prevalence and IgE levels and the factors associated with the region in which the children population live, in Sirnak province, in the eastern of Turkey. A total of 357 symptomatic children aged 4 to 12 years, who were admitted to the Paediatric Polyclinic of Sirnak State Hospital, were examined prospectively. The collected stool samples were examined with direct wet-mount and concentration method under light microscope. In addition, total serum IgE levels were compared among 223 children with parasitic disease and 134 children without parasitic disease. One or more intestinal...
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Objective. To reveal the relationship between interferon-gamma release assay (IGRA) test (Standard ETB-Feron ELISA (TBF)) results performed within 12 months before the COVID-19 pandemic and the frequency of COVID-19 infections and the... more
Objective. To reveal the relationship between interferon-gamma release assay (IGRA) test (Standard ETB-Feron ELISA (TBF)) results performed within 12 months before the COVID-19 pandemic and the frequency of COVID-19 infections and the severity of COVID-19. Methods. The retrospective TBF test results and contact information of 684 patients aged over 18 years who underwent TBF testing between March 11th, 2019, and March 10th, 2020, were obtained. Of the 684 patients contacted by phone, 365 agreed to participate in the study and were enrolled. The patients were divided into three groups (TBF test positive, negative, and indeterminate). The data obtained from the questionnaire were compared statistically. Results. According to the TBF test results, positive (n = 51, 14%), negative (n = 286, 78.3%), and indeterminate (n = 28, 7.7%) groups were compared. The frequency of COVID-19 infections in the indeterminate group was found significantly higher than that in the positive and negative gr...
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Bifidobacteria are beneficial bacteria for humans. These bacteria are particularly effective at protecting against infectious diseases and modulating the immune response. It was shown that in newborns, the fecal distribution of the... more
Bifidobacteria are beneficial bacteria for humans. These bacteria are particularly effective at protecting against infectious diseases and modulating the immune response. It was shown that in newborns, the fecal distribution of the colonizing Bifidobacterium species influences the prevalence of allergic diseases. This study aimed to compare the faecal Bifidobacterium species of allergic children to those of healthy children to detect species level differences in faecal distribution. Stool samples were obtained from 99 children between 0 and 3 years of age whose clinical symptoms and laboratory reports were compatible with atopic dermatitis and allergic asthma. Samples were also obtained from 102 healthy children who were similar to the case group with respect to age and sex. Bifidobacteria were isolated by culture and identified at the genus level by API 20 A. In addition, 7 unique species-specific primers were used for the molecular characterization of bifidobacteria. The McNemar t...
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Objectives: The aim of this study was to determine the distribution of ticks in the Marmara region and the ratio of Borrelia burgdorferi infection caused by ticks (Ixodes spp). Methods: Live ticks collected from the Marmara region were... more
Objectives: The aim of this study was to determine the distribution of ticks in the Marmara region and the ratio of Borrelia burgdorferi infection caused by ticks (Ixodes spp). Methods: Live ticks collected from the Marmara region were brought to the laboratory. Ticks on patients who came to our laboratory with tick bites were removed using forceps. These ticks were examined by stereo microscope and identified. The patients were informed about ticks and tick-borne diseases. People bitten by Hyalomma ticks were directed to the infectious diseases clinic and were kept under observation. The presence of B. burgdorferi in these ticks was determined using dark-field microscopy and B. burgdorferi was cultured in vitro using Barbour-Stoenner-Kelly (BSK)-H medium. Results: Of the 462 ticks in our sample, 208 (45%) belonged to the genus Ixodes, 105 (22.7%) to Boophilus, 63 (13.6%) to Rhipicephalus, 61 (13.2%) to Hyalomma, 20 (4.3%) to Dermacentor, and 5 (1.1%) to Haemaphysalis. Ixodes was th...
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The aim of this study was to show the larval debridement therapy can be an initial therapy for chronically necrotic wounds with or without enfection. Steril larvae were put into wound directly or in a steril bag, and for wounds, each of... more
The aim of this study was to show the larval debridement therapy can be an initial therapy for chronically necrotic wounds with or without enfection. Steril larvae were put into wound directly or in a steril bag, and for wounds, each of which is 1 cm on average, 6-7 larvae were applied on five patients. Therapy was applied once or twice a week depending on necrotic tissue amount, after 24-48 hours larvae were taken away from wound surface. This procedure continued sustainably until all necrotic tissues were removed from the wound. Larval debridement treatment (LDT) was applied to 5 patients. The average age of patients was calculated as 43.8/years old, average treatment time was found 15.2 days. The average wound size was found 9,66 centimeters. In two cases, Pseudomonas aeruginosa was isolated from tissue defects. Before the treatment we gave antibiotics to all patient and we performed debridement treatment mechanically on two patients. The cases were ceserian section, hysterectomy...
Objective: The objective of this study is to review the studies on leishmaniasis since 40 years and to evaluate the increasing contribution of Turkey in terms of the studies on leishmaniasis globally. Materials and Methods: The literature... more
Objective: The objective of this study is to review the studies on leishmaniasis since 40 years and to evaluate the increasing contribution of Turkey in terms of the studies on leishmaniasis globally. Materials and Methods: The literature data included all leishmaniasis research publications in the Web of Science database between January 1980 and December 2019. Language or document type limitations were not used. To evaluate the publication and research effect of Turkey, in the analysis function of the database, “Turkey” was chosen as the country and the publications on this topic were recorded based on their subject areas and yearly counts. Results: A total of 39373 publications were indexed from 1980 to 2019 in the Web of Science database. Among these publication, 541 were from Turkey. Language analysis of the publications revealed that English had the largest percentage (95.7%). When the worldwide geographical distribution of studies on leishmaniasis was analyzed, United States o...
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INTRODUCTION Leishmaniasis is a vector-borne disease caused by flagellated protozoans of the genus Leishmania. This study aimed to evaluate the epidemiological status of Visceral Leishmaniasis (VL) and Cutaneous Leishmaniasis (CL) among... more
INTRODUCTION Leishmaniasis is a vector-borne disease caused by flagellated protozoans of the genus Leishmania. This study aimed to evaluate the epidemiological status of Visceral Leishmaniasis (VL) and Cutaneous Leishmaniasis (CL) among patients admitted to a university hospital in Istanbul, located in western Turkey. METHODOLOGY This study included 160 and 77 patients with a pre-diagnosis of VL and CL, respectively, between January 2001 and December 2017. Detailed demographic data, including age, gender, nationality and the number and location of lesions were collected and recorded from the patient registries. RESULTS Among 160 bone marrow specimens that suspected as VL, 22 (13.7%) of the specimens that were evaluated with both culture and Giemsa staining detected as positive. Furthermore, 29 (37.7%) of the 77 patients suspected for CL showed evidence of Leishmania. CONCLUSIONS The increase in human immigration from neighbouring countries (with a high incidence of leishmaniasis) to...
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I would like to mention an issue discussed constantly these days in the light of scientific data. It would be best to start by answering a question everyone is curious about before we start the main topic. Will this virus go away? The... more
I would like to mention an issue discussed constantly these days in the light of scientific data. It would be best to start by answering a question everyone is curious about before we start the main topic. Will this virus go away? The answer is simply “No”. The virus will probably continue to exist together with us; however, one day, the pandemic will end. The only answer we can give to the question of how this pandemic will end is “scientific and social fight”. All countries throughout the entire world are fighting to control the spread of SARS-CoV-2. The most important among these global fights is proper medical treatment in addition to an early, rapid and accurate diagnosis. Besides, another aspect that will crown this fight is the vaccination programs. The success of this fight depends on people’s accepting the vaccines. A total of 10 of the vaccine candidates, which numbered more than 165 developed within a record time period, have achieved approval after their final stages of ...
Background Schistosomiasis caused by Schistosoma parasites is one of the most common parasitic infections worldwide. Genetic regulation of the genus Schistosoma, which has different developmental stages throughout its life, is quite... more
Background Schistosomiasis caused by Schistosoma parasites is one of the most common parasitic infections worldwide. Genetic regulation of the genus Schistosoma, which has different developmental stages throughout its life, is quite complex. In these parasites, thousands of long noncoding RNAs (lncRNAs) estimated to be functional were identified. Identifying the transcripts expressed in common and detecting their functions for better understanding of the role of these lncRNAs require a comparative study. Methods Assembled RNA-seq datasets belonging to S. mansoni and S. haematobium were obtained from the National Center for Biotechnology. A basic local alignment search tool (BLASTN) analysis was conducted against previously constructed lncRNA library to identify the common lncRNAs between two species. LncRNAs target genes and their gene ontology annotation was performed. Results In S. mansoni and S. haematobium, 5132 and 3589 lncRNA transcripts were detected, respectively. These two ...
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Bu calismada amacimiz Larvadebridmantedavisinin enfekte olmus ya da olmamis kronik nekrotik yaralar icin ilk tedavi olabilecegini gostermekti. Steril larvalar her biri bir santimetrekare yaraya sahip olan bes hastanin yaralarina ortalama... more
Bu calismada amacimiz Larvadebridmantedavisinin enfekte olmus ya da olmamis kronik nekrotik yaralar icin ilk tedavi olabilecegini gostermekti. Steril larvalar her biri bir santimetrekare yaraya sahip olan bes hastanin yaralarina ortalama 6-7 larva olmak suretiyle direkt ya da steril poset icerisinde konulmustur. Larvalar yara yuzeyinden alindiktan 24-48 saat sonra tedavi, nekrotik doku miktarina bagli olarak haftada bir ya da iki kez uygulanmistir. Bu prosedur tum nekrotik dokular yaradan alinana kadar araliksiz bir sekilde surdurulmustur. Larvadebridmantedavisi (LDT) 5 hastaya uygulanmistir. Ortalama hasta yasi 43.8, ortalama tedavi suresi 15.2 gun seklinde bulunmustur. Ortalama yara buyuklugu 9.66 cm'dir. Iki vakada, pseudomonas aeruginosa doku defektinden izole edilmistir. Tedaviden once tum hastalara antibiyotik verilmis, iki hasta uzerinde debridman tedavisi mekanik olarak uygulanmistir. Vakalar sezeryan, histerektomi, salpingooferektomi ve radikal vulvektomidir. Uc vakada ...
Mediastinal fat has been suggested to be associated with cardiovascular diseases such as carotid stiffness, atherosclerosis and coronary artery calcification. We investigated the possible role of Ad-36-induced obesity in the pathogenesis... more
Mediastinal fat has been suggested to be associated with cardiovascular diseases such as carotid stiffness, atherosclerosis and coronary artery calcification. We investigated the possible role of Ad-36-induced obesity in the pathogenesis of the coronary artery disease (CAD). Ad-36 DNA was investigated in the anterior mediastinal fat tissue samples of obese adults with CAD. Seventy-five obese adults with left main coronary artery (LMCA) disease, 28 non-obese adults with valvular heart diseases, and 48 healthy individuals without cardiovascular problems were included as the obese patient group (OPG), non-obese patient group (NOG) and healthy control group (HCG), respectively. We also simultaneously investigated Ad-36 antibodies by serum neutralization test (SNA), and measured leptin and adinopectin levels. Ad-36 antibodies were detected only in 10 patients (13.3%) within the 75 OPG. A statistically significant difference was detected between OPG, NOG and HCG in terms of Ad-36 antibody...
Akademisyen Yayinevi yoneticileri, yaklasik 30 yillik yayin tecrubesini, kendi tuzel kisiliklerine aktararak uzun zamandan beri, ticari faaliyetlerini surdurmektedir. Anilan sure icinde, basta saglik ve sosyal bilimler, kulturel ve... more
Akademisyen Yayinevi yoneticileri, yaklasik 30 yillik yayin tecrubesini, kendi tuzel kisiliklerine aktararak uzun zamandan beri, ticari faaliyetlerini surdurmektedir. Anilan sure icinde, basta saglik ve sosyal bilimler, kulturel ve sanatsal konular dahil 750 kitabi yayimlamanin gururu icindedir. Uluslararasi yayinevi olmanin alt yapisini tanimlayan Akademisyen, Turkce ve yabanci dillerde yayin yapmanin yaninda, kuresel bir marka yaratmanin pesindedir.Bilimsel ve dusunsel calismalarin kalici belgeleri sayilan kitaplar, bilgi kayit ortami olarak yuzlerce yilin taniklaridir. Matbaanin icadiyla varolusunu saglam temellere oturtan kitabin gelecegi, her ne kadar yeni buluslarin yorungesine tasinmis olsa da, daha uzun sure hayatimizda yer edinecegi muhakkaktir.
Aim: The interaction of various adipokines secreted from adipose tissue during Adenovirus 36 (Ad36) infection may cause gynecomastia. In this study, we aimed to evaluate the association between Ad36 and gynecomastia development by... more
Aim: The interaction of various adipokines secreted from adipose tissue during Adenovirus 36 (Ad36) infection may cause gynecomastia. In this study, we aimed to evaluate the association between Ad36 and gynecomastia development by determining DNAs and antibodies of Ad36, adiponectin, leptin, and IL6 levels. Materials and Methods: Patient and control groups were composed of from 33 male adults with gynecomastia and 15 male adults with anatomic disorders without gynecomastia, respectively. Serum neutralization assay (SNA) was used for the determination of Ad36 antibodies and PCR method was used for the determination of Ad36 DNA. Results: No Ad36 DNA was found in the breast tissue samples. Ad36 antibody levels were detected in the patient group with gynecomastia higher than the control group (p<0.05). The leptin and adiponectin levels were increased and decreased in the Ad36 positive patient group individuals. IL-6, cholesterol, and triglyceride levels were not detected to ...
Following the emergence of severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) and using only PCR for diagnosis, antibody tests have been rapidly developed by various commercial companies. There are differences between the... more
Following the emergence of severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) and using only PCR for diagnosis, antibody tests have been rapidly developed by various commercial companies. There are differences between the sensitivity and specificity of these tests due to the usage of different viral target proteins and antibody subclasses. In order to evaluate the diagnostic use of these tests, we aimed to examine the diagnostic performance, especially sensitivity and specificity, of SARS-CoV-2 IgM, IgA and IgG tests of various companies (Abbott, Roche, Euroimmun, Dia.Pro, Anshlabs, Vircell, UnScience and RedCell), which have different principles (ECLIA/CLIA, EIA, LFA). Current (n= 180) and past (n= 180) COVID-19 patients with clinical and molecular diagnosis of COVID-19 admitted to Istanbul University-Cerrahpaşa, Cerrahpaşa Faculty of Medicine Hospital, Pandemic Polyclinic with suspected COVID-19 infection, were included in our study. The patients admitted within the firs...
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Objective: Nowadays, antibiotic resistance is defined as a global public health problem. The World Health Organization aims to raise awareness among users and policy makers by grouping both critically importantl antibiotics and resistant... more
Objective: Nowadays, antibiotic resistance is defined as a global public health problem. The World Health Organization aims to raise awareness among users and policy makers by grouping both critically importantl antibiotics and resistant bacteria. In this study, it is aimed to make the bibliometric evaluation of of the literature on the resistance of colistin. Method: Specific keywords associated with colistin resistance were used in Scopus database covering the period between 1947, and 2019. Quantitative and qualitative analysis of retrieved data were presented using appropriate bibliometric indicators, the number, and language of the publications, the countries that contributed mostly to the literature, the most influential institutions, the most frequently cited publications, active writers and active journals. Results: A total of 1454 articles were evaluated. Although the number of articles showed an increase from 2009 to 2015, it was determined that the increases after 2015 wer...
Lucilia sericata larvae have been successfully used on healing of wounds in the diabetics. However, the involvement of the extraction/secretion (ES) products of larvae in the treatment of diabetic wounds is still unknown. Activator... more
Lucilia sericata larvae have been successfully used on healing of wounds in the diabetics. However, the involvement of the extraction/secretion (ES) products of larvae in the treatment of diabetic wounds is still unknown. Activator protein-1 (AP-1) transcription, composed of c-jun and c-Fos proteins, has been shown to be the principal regulator of multiple MMP transcriptions under a variety of conditions, also in diabetic wounds. Specifically, MMP-2 and MMP-9's transcriptions are known to be modulated by AP-1. c-jun has been demonstrated to be a repressor of p53 in immortalized fibroblasts. The aim of the present study is to investigate the effects of L. sericata ES on the expression of AP-1 (c-jun), p53, MMP-2, and MMP-9 in wound biopsies dissected from streptozotocin induced diabetic rats. The expression levels of MMP-2, MMP-9, c-jun and p53 in dermal tissues were determined at days 0, 3, 7 and 14 after wounding, using immunohistochemical analysis and quantitative real-time PC...
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Nitroimidazoles, which are drugs that are used to effectively treat Trichomonas vaginalis, alter the structure of the T. vaginalis cell membrane, penetrate into its cytoplasm and nucleus and block cellular metabolism. In this study, we... more
Nitroimidazoles, which are drugs that are used to effectively treat Trichomonas vaginalis, alter the structure of the T. vaginalis cell membrane, penetrate into its cytoplasm and nucleus and block cellular metabolism. In this study, we observed the morphological changes that occurred in T. vaginalis during in vitro exposure to 1.3 μg/mL of ornidazole at various time intervals ranging from 10 minutes to 10 hours. Vaginal and urethral secretion samples from suspected T. vaginalis cases were inoculated into Cysteine Peptone Liver Maltose medium. In 18 sterile tubes, 9.5 mL of this solution were mixed with 0.5 mL of ornidazole. The periods of contact between ornidazole and T. vaginalis ranged from 10 minutes to 10 hours. The first change was vacuolization, which started in the 10th minute of exposure. The glycogen particles started to diminish in the 20th minute. During exposure to 1.3 mg/L of ornidazole, cell lysis began in the 30th minute and accelerated towards the 60th minute (p <...