- Dr. Poernomo was born in 1958 and obtained a PhD Degree from The University of New South Wales, Australia. He started... moreDr. Poernomo was born in 1958 and obtained a PhD Degree from The University of New South Wales, Australia. He started his career as a Research Scientist in 1982 and held the position as Director General of Agency for Marine and Fisheries Research and Development during 2013-2015. While serving as Senior Advisor on Economic, Social and Cultural Affairs to the Minister of Marine and Fisheries , he was assigned also as Special Envoy of Minister of Marine Affairs and Fisheries on Climate Change. He is also Council Director member of South-East Asia Fisheries Development Centre (SEAFDEC). He has published almost 100 papers on fisheries research and policies. He was recently awarded SEAFDEC Most Distinguished Person in the commemoration of 50th SEAFDEC Aniversary. After his retirement as Senior Advisor, he is now a Senior Lecturer in the Fisheries University . His fields of interest are Fisheries Management, and Seafood Quality and safety.edit
Six Sigma has been utilised to analyse defects of fish steak processing at PT X located in North Jakarta. The tools of the Six Sigma implemented in the study were Define, Measure, Analyse and Improve. The observation was conducted for two... more
Six Sigma has been utilised to analyse defects of fish steak processing at PT X located in North Jakarta. The tools of the Six Sigma implemented in the study were Define, Measure, Analyse and Improve. The observation was conducted for two months in 2020 before the company was temporarily shut down due to Covid-19 pandemic. Four defects were detected during the study, ie under size fish, softened meat, off odor and green coloration of meat, totaling 2,446 kg out of 15,080 kg of fish. Further analysis showed that the Sigma level was 3.24 with a Defect per Million Opportunities (DPMO) of 40,739. Some improvements were recommended based on cause-effect diagram.
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Screening of fibrinolytic activity of tempeh extract derived from Rhizopus oligosporus ATCC 6010 fermentation has been investigated. The fermentation used Glycine Soja Sieb. et Zucc, Canavalia ensiformis, Phaseolus radiaus L., Lablab... more
Screening of fibrinolytic activity of tempeh extract derived from Rhizopus oligosporus ATCC 6010 fermentation has been investigated. The fermentation used Glycine Soja Sieb. et Zucc, Canavalia ensiformis, Phaseolus radiaus L., Lablab purpureus, Glysine max (L) Merrill, Glycine Soja Sieb. et Zucc, and Vigna unguiculata as substrates. Nattokinase and phosphate buffer 0,05 M pH 5,0 was used as positive and negative control respectively. The proteolytic and fibrinolytic activity of fermentation product used five kinds of legumes has been compared each other. The Glycine Soja Sieb. et Zucc exhibited the highest activity with proteolytic and fibrinolytic index of the crude enzyme is 3.00 ± 1.58 and 3,07 ± 0,15 respectively. Ratio of the proteolytic and fibrinolytic index between the crude enzyme and nattokinase is 112,36 % and 88,14% respectively.
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Abstrak: Desa Pagedangan Ilir merupakan desa yang mempunyai potensi yang sangat besar di bidang perikanan khususnya baby crab. Perlu pemanfaatan potensi baby crab di Desa Pagedangan Ilir dengan mengolahnya untuk meningkatkan nilai tambah... more
Abstrak: Desa Pagedangan Ilir merupakan desa yang mempunyai potensi yang sangat besar di bidang perikanan khususnya baby crab. Perlu pemanfaatan potensi baby crab di Desa Pagedangan Ilir dengan mengolahnya untuk meningkatkan nilai tambah baby crab. Tujuan dari kegiatan pengabdian ini adalah untuk meningkatkan keterampilan masyarakat Desa Pagedangan Ilir dalam mengolah baby crab krispi. Kegiatan pengabdian dilakukan dengan dua tahap, tahap pertama yaitu pemberian materi yang dilakukan melalui ceramah dan diskusi secara tatap muka (luring) sedangkan tahap kedua yaitu kegiatan praktik pengolahan baby crab krispi yang dilakukan secara langsung. Kegiatan pengabdian ini diikuti oleh 15 orang peserta dengan 3 (tiga) kelompok dan masing-masing kelompok terdapat 5 orang peserta, evaluasi dilakukan setelah semua kegiatan pelatihan selesai. Tahapan kegiatan ini meliputi koordinasi awal kegiatan, pembukaan, pemberian materi (teori), praktik pengolahan baby crab dan evaluasi. Hasil pengabdian me...
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Penilaian kesegaran ikan mas secara sensori perlu dilakukan karena penilaian tersebut bersifat mudah dan murah. Penilaian sensori kesegaran ikan mas menggunakan metode Demerit Point Score (DPS) belum pernah dilakukan sebelumnya.... more
Penilaian kesegaran ikan mas secara sensori perlu dilakukan karena penilaian tersebut bersifat mudah dan murah. Penilaian sensori kesegaran ikan mas menggunakan metode Demerit Point Score (DPS) belum pernah dilakukan sebelumnya. Penelitian ini dilakukan dengan cara mengamati perubahan sensori ikan mas (Cyprinus carpio) menggunakan scoresheet DPS baku selama 24 jam penyimpanan suhu ruang dengan interval pengamatan setiap 4 jam sekali. Skor DPS ikan mas mengalami peningkatan seiring dengan lamanya waktu penyimpanan. Hasil pengamatan menunjukkan bahwa ikan mas masih layak konsumsi pada jam ke-8 penyimpanan suhu ruang dengan skor DPS sebesar 15,5. Ciri-ciri ikan mas pada penyimpanan jam ke 8 yaitu kenampakan cerah, sisik agak mudah lepas, lendir tipis dan agak keruh, ikan mengalami rigor/kekakuan badan, mata mulai agak berkabut, pupil mata masih terlihat, bentuk mata agak cekung, mata tidak berdarah, warna insang menjadi agak pudar, lendir insang tipis agak keruh serta bau insang menja...
To compete in the present and future, the newly constructed Muara Baru modern fish market, called Pasar Ikan Modern Muara Baru (PIM Muara Baru), requires an effective management approach. This research aims to examine different management... more
To compete in the present and future, the newly constructed Muara Baru modern fish market, called Pasar Ikan Modern Muara Baru (PIM Muara Baru), requires an effective management approach. This research aims to examine different management techniques for PIM Muara Baru utilizing a Strength, Weakness, Opportunity, and Threat (SWOT) analysis. The findings indicate that an aggressive strategy is required. Alternative management strategies include improving facilities and adding products sold at the food court, assigning special employees to promote and implement more intensive and innovative promotional activities on social media, collaborating with the government in optimizing Gemarikan (a national program to popularize eating fish), cooperating with online business partners and increasing merchants' capacity to transact online, adding facilities and improving the service quality. The outcomes of the alternative techniques are offered for PIM Muara Baru management to consider. Keyw...
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Pada tahun 2019 Kementerian Kelautan dan Perikanan membangun pasar ikan modern di Kabupaten Bandung yaitu pasar ikan modern Sabilulungan. Pasar ikan modern Sabilulungan yang baru dibangun membutuhkan strategi untuk pengelolaan yang tepat... more
Pada tahun 2019 Kementerian Kelautan dan Perikanan membangun pasar ikan modern di Kabupaten Bandung yaitu pasar ikan modern Sabilulungan. Pasar ikan modern Sabilulungan yang baru dibangun membutuhkan strategi untuk pengelolaan yang tepat untuk menjamin tercapainya tujuan dan PIM yang mampu bersaing di masa sekarang dan masa yang akan datang. Penelitian ini bertujuan untuk menyusun strategi pengelolaan pasar ikan modern Sabilulungan. Metode penelitian menggunakan teknik purposive sampling kemudian dianalisis menggunakan analisis SWOT. Hasil penelitian menunjukkan bahwa strategi yang diperlukan adalah aggressive strategy. Alternatif strategi pengelolaan antara lain: (1) menambah fasilitas foodcourt dan menambah produk yang dijual di foodcourt (2) bekerja sama dengan pengelola lokasi wisata dalam mempromosikan PIM Sabilulungan (3) bekerja sama dengan pemerintah untuk mendorong masyarakat dalam meningkatkan konsumsi ikan di PIM Sabilulungan (4) menyediakan SDM khusus untuk melakukan pr...
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Susyi adalah pangan siap konsumsi asal Jepang, merupakan kombinasi nasi yang diberi asam dan ikan mentah. Umumnya susyi terdiri dari beberapa jenis, namun terdapat dua jenis susyi yang sangat dikenal yaitu nigiri (nasi dengan ikan mentah... more
Susyi adalah pangan siap konsumsi asal Jepang, merupakan kombinasi nasi yang diberi asam dan ikan mentah. Umumnya susyi terdiri dari beberapa jenis, namun terdapat dua jenis susyi yang sangat dikenal yaitu nigiri (nasi dengan ikan mentah di atasnya) dan maki (nasi dengan ikan mentah di dalamnya). Ikan dalam susyi tidak mengalami pemanasan sehingga rentan terhadap kontaminasi patogen, di antaranya Staphylococcus aureus dan Bacillus cereus. Kedua bakteri ini telah dilaporkan sebagai penyebab Kejadian Luar Biasa (KLB) di Indonesia, namun keberadaannya di dalam susyi belum pernah dilaporkan. Penelitian bertujuan untuk menentukan prevalensi dan tingkat kontaminasi S. aureus dan B. cereus pada susyi di tingkat ritel di wilayah Jabodetabek (Jakarta, Bogor, Depok, Tangerang, dan Bekasi). Sebanyak 120 sampel terdiri dari nigiri (n=57) dan maki (n=63) diperoleh dari ritel di Jabodetabek. Identifikasi dilakukan dengan metode Polymerase Chain Reaction (PCR), dengan target gen nuc (S. aureus) da...
Sargassum sp. has been known as a natural source of antioxidants. In Indonesia, Sargassum sp. grows very well yet it has not been utilized optimally. Sargassum sp. could be utilized as herbal tea through the drying process. However, the... more
Sargassum sp. has been known as a natural source of antioxidants. In Indonesia, Sargassum sp. grows very well yet it has not been utilized optimally. Sargassum sp. could be utilized as herbal tea through the drying process. However, the process can affect its antioxidants; therefore, this study aimed to determine the effects of drying conditions on phenolic contents and antioxidant activity of Sargassum tea. Fresh Sargassum sp. was harvested from Lima Island, Serang, Banten, Indonesia. Upon arrival at the laboratory, Sargassum sp. was washed with tap water then soaked in 5% lime solution (1:8) for 6 hours. The Sargassum was then dried at three different conditions (sun drying, air oven at 40 °C and 55 °C). The dried Sargassum sp. (2 g) was packed in the tea bag then brewed in 150 ml boiled water for 3 minutes for analysis; i.e., total phenolic contents, antioxidant activity, and sensory properties (color, taste, and flavor). The results showed that the Sargassum tea dried at 55°C ha...
Sargassum sp. has been known as a natural source of antioxidants. In Indonesia, Sargassum sp. grows very well yet it has not been utilized optimally. Sargassum sp. could be utilized as herbal tea through the drying process. However, the... more
Sargassum sp. has been known as a natural source of antioxidants. In Indonesia, Sargassum sp. grows very well yet it has not been utilized optimally. Sargassum sp. could be utilized as herbal tea through the drying process. However, the process can affect its antioxidants; therefore, this study aimed to determine the effects of drying conditions on phenolic contents and antioxidant activity of Sargassum tea. Fresh Sargassum sp. was harvested from Lima Island, Serang, Banten, Indonesia. Upon arrival at the laboratory, Sargassum sp. was washed with tap water then soaked in 5% lime solution (1:8) for 6 hours. The Sargassum was then dried at three different conditions (sun drying, air oven at 40 °C and 55 °C). The dried Sargassum sp. (2 g) was packed in the tea bag then brewed in 150 ml boiled water for 3 minutes for analysis; i.e., total phenolic contents, antioxidant activity, and sensory properties (color, taste, and flavor). The results showed that the Sargassum tea dried at 55°C ha...
Shrimp is an important non-oil commodity for foreign trade in Indonesia. However, rejection of shrimp exports by the importing countries is still commonly encountered. In 2011, the USFDA recorded two cases of Salmonella spp. contamination... more
Shrimp is an important non-oil commodity for foreign trade in Indonesia. However, rejection of shrimp exports by the importing countries is still commonly encountered. In 2011, the USFDA recorded two cases of Salmonella spp. contamination in shrimp products from two shrimp processing companies in Indonesia. Analysis of Salmonella spp. in seafood is generally performed using a conventional method which takes at least 5 days. The objective of the study is to get a Salmonellae rapid detection method in shrimp by PCR. In this study, optimization of PCR protocol method to detect Salmonella invA gene was conducted using six different annealing temperatures (59, 59.5, 60.8, 62, 64 and 64.5°C). The results showed that 64°C was the optimum annealing temperature to detect the 284 bp fragment of Salmonella invA gene. The PCR based detection method has a DNA detection limit of 27.81g/mL and 10°CFU/mL of viable salmonellae with 100% specificity. The PCR protocol is capable of detecting six diff...
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Shrimp is an important non-oil commodity for foreign trade in Indonesia. However, rejection of shrimp exports by the importing countries is still commonly encountered. In 2011, the USFDA recorded two cases of Salmonella spp. contamination... more
Shrimp is an important non-oil commodity for foreign trade in Indonesia. However, rejection of shrimp exports by the importing countries is still commonly encountered. In 2011, the USFDA recorded two cases of Salmonella spp. contamination in shrimp products from two shrimp processing companies in Indonesia. Analysis of Salmonella spp. in seafood is generally performed using a conventional method which takes at least 5 days. The objective of the study is to get a Salmonellae rapid detection method in shrimp by PCR. In this study, optimization of PCR protocol method to detect Salmonella invA gene was conducted using six different annealing temperatures (59, 59.5, 60.8, 62, 64 and 64.5°C). The results showed that 64°C was the optimum annealing temperature to detect the 284 bp fragment of Salmonella invA gene. The PCR based detection method has a DNA detection limit of 27.81g/mL and 10°CFU/mL of viable salmonellae with 100% specificity. The PCR protocol is capable of detecting six diff...
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This paper reports an analysis of formaldehyde in dried salted short mackerel (Rastrelliger brachysoma) which was intentionally added with formaldehyde compared to those that was not. The purpose was to see whether salting could cover up... more
This paper reports an analysis of formaldehyde in dried salted short mackerel (Rastrelliger brachysoma) which was intentionally added with formaldehyde compared to those that was not. The purpose was to see whether salting could cover up the illegal use of formaldehyde. Mackerel was obtained from one day fishing landed at Karangantu Fishing Port, Serang-West Java. The fish was transported in ice by road (3 hours) to the laboratory in Jakarta, and divided into two groups upon arrival. One group was soaked in 3% (w/v) formaldehyde solution for 30 minutes, while another group was not (control treatment). They were then salted in saturated brine for 12 and 24 hours at ambient temperature and sun dried subsequently to 40% moisture content or less. The formaldehyde content of raw materials was in the range of 1.4-1.7 ppm, indicating that natural formaldehyde was present in the fish. Soaking in 3% formaldehyde solution for 30 minutes significantly increased fish formaldehyde content from 1...
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Kebijakan one map policy yang telah didengungkan sejak 2012 mulai bisa menyatukan kementerian/lembaga terkait untuk dapat bekerja secara bersama-sama. Salahsatunya adalah Kementerian Kelautan dan Perikanan (KKP) yang melalui Balitbang KP... more
Kebijakan one map policy yang telah didengungkan sejak 2012 mulai bisa menyatukan kementerian/lembaga terkait untuk dapat bekerja secara bersama-sama. Salahsatunya adalah Kementerian Kelautan dan Perikanan (KKP) yang melalui Balitbang KP (Badan Penelitian dan Pengembangan Kelautan dan Perikanan) berhasil menerbitkan Peta Sebaran Terumbu Karang Nasional oleh Pokja Pemetaan Sumber Daya Pesisir pada 2013. Disusul kemudian dengan Peta Karakteristik Laut Nasional dan Peta Habitat Lamun Nasional. Peta-peta tersebut merupakan langkah awal yang nyata dan akan ditindaklanjuti dengan pembaruan-pembaruan data dan informasi. Balitbang KP secara aktif rutin di setiap tahun melakukan survei laut, pengukuran parameter oseanografi pesisir, dan akuisisi data deret waktu karakteristik massa air laut. Leaflet ini berisikan informasi mulai dari One Map Policy, kendala, dukungan hingga peluncuran One Map.
Southeast Asian Fisheries Development Center The global demand for tunas has been dramatically growing in recent years reflecting a shift of consumers’ preference to food fish as protein source. The world tuna markets have substantially... more
Southeast Asian Fisheries Development Center The global demand for tunas has been dramatically growing in recent years reflecting a shift of consumers’ preference to food fish as protein source. The world tuna markets have substantially expanded and diversified into tuna sashimi and canned tuna. During the last several decades, Japan had been almost the only market for sashimi tuna, but nowadays, people in many countries of the world also prefer to eat tuna sashimi. The status of tuna stocks of the world depend on the regions/areas as well as on the species. Although some species are reported to be over-exploited, production of other species is continuously stable as a result of conservation and management efforts by Tuna Regional Fisheries Management Organizations (tuna RFMOs). Tunas are commercially-important fishery resources in the Southeast Asian region, providing products for export as well as for domestic consumption. As reported, the total tuna production from Southeast Asia...
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Ikan patin merupakan salah satu komoditas unggulan yang ditetapkan oleh Kementerian Kelautan dan Perikanan (KKP) dalam program percepatan industrialisasi perikanan budi daya. KKP terus melakukan pemantauan dan mendorong produksi patin... more
Ikan patin merupakan salah satu komoditas unggulan yang ditetapkan oleh Kementerian Kelautan dan Perikanan (KKP) dalam program percepatan industrialisasi perikanan budi daya. KKP terus melakukan pemantauan dan mendorong produksi patin nasional dengan mendirikan sentra patin di beberapa provinsi, salah satunya Provinsi Jambi. Mengingat adanya potensi pengembangan budi daya patin yang besar, sejak 2017 Provinsi Jambi dicanangkan menjadi sentra ikan nasional, tetapi hingga saat ini belum terealisasi. Penelitian ini bertujuan memberikan gambaran terkini kondisi perikanan patin di Provinsi Jambi. Penelitian dilaksanakan pada bulan Februari hingga Agustus 2019 menggunakan metode deskriptif, dengan pendekatan studi kasus. Proses pengambilan data dilakukan dengan observasi dan wawancara terhadap key informan. Responden dalam penelitian ini adalah pelaku usaha yang ditentukan dengan Metode Slovin, responden konsumen serta pembuat kebijakan yang ditentukan dengan purposive sampling. Kondisi i...
Jakarta Utara merupakan salah satu kawasan industri perikanan di Indonesia yang menghasilkan udang olahan sebagai komoditas ekspor. Namun, unit pengolahan ikan (UPI) dihadapkan dengan adanya permasalahan mutu, jumlah, ketersediaan bahan... more
Jakarta Utara merupakan salah satu kawasan industri perikanan di Indonesia yang menghasilkan udang olahan sebagai komoditas ekspor. Namun, unit pengolahan ikan (UPI) dihadapkan dengan adanya permasalahan mutu, jumlah, ketersediaan bahan baku, dan transportasi yang memiliki peranan dalam manajemen rantai pasok. Penelitian ini bertujuan untuk mengidentifikasi pelaku usaha yang terlibat dalam rantai pasok udang budi daya pada UPI di Jakarta Utara dan biaya transportasi dalam pendistribusian udang. Penelitian dilaksanakan pada bulan Agustus–Desember 2019 di Cirebon, Indramayu, Lampung Selatan, Pesawaran, Tanggamus, Kendal, dan Rembang. Analisis data yang digunakan adalah analisis deskripsi. Pengumpulan data dilakukan melalui survei, observasi, dan wawancara. Penentuan responden menggunakan snowball sampling. Responden awal yang terlibat adalah UPI di Jakarta Utara. Responden dalam penelitian ini meliputi dua orang dari UPI, tujuh orang pemasok, 14 orang pembudi daya, dan lima orang pemb...
Udang merupakan komoditas unggulan ekspor Indonesia yang memerlukan bahan baku yang berkualitas dan aman. Untuk mendapatkan bahan baku udang yang sesuai, seluruh anggota rantai pasok harus menerapkan persyaratan jaminan mutu dan keamanan... more
Udang merupakan komoditas unggulan ekspor Indonesia yang memerlukan bahan baku yang berkualitas dan aman. Untuk mendapatkan bahan baku udang yang sesuai, seluruh anggota rantai pasok harus menerapkan persyaratan jaminan mutu dan keamanan hasil perikanan sesuai Kepmen KP Nomor: 52A/KEPMEN-KP/2013. Tujuan penelitian ini adalah mengevaluasi penerapan persyaratan jaminan mutu dan keamanan hasil perikanan sepanjang rantai pasok bahan baku udang untuk unit pengolahan ikan (UPI) di Jakarta Utara. Dua UPI telah dipilih menjadi responden untuk dirunut ke hulu mengenai pemenuhan persyaratan dimaksud. Pengumpulan data dilakukan melalui survei, observasi dan wawancara kepada UPI, pengumpul/pemasok, pembudidaya dan pembenih. Analisis kesenjangan dan uji korelasi berganda digunakan untuk menilai kesesuaian penerapan persyaratan jaminan mutu dan keamanan hasil perikanan. Hasil identifikasi menunjukkan masih terdapat kesenjangan penerapan yang dilakukan oleh pembudidaya dan pengumpul/pemasok dengan...
T. soro, in Indonesia called salmon van Java is of high economic value, and due to high demand, its culture has been intensively studied and developed. This study aimed to assess the nutritional value of wild and cultured T. soro. The... more
T. soro, in Indonesia called salmon van Java is of high economic value, and due to high demand, its culture has been intensively studied and developed. This study aimed to assess the nutritional value of wild and cultured T. soro. The fish’s proximate compositions, minerals, as well as amino and fatty acid profiles were analyzed. A t-test analysis was used to identify differences between treatments. Results showed that the fat content of wild T. soro was higher than that of cultured fish, but the protein, water, and ash contents between the two groups were not significantly different (p >0.05). T. soro was considered a lean fish with higher concentrations of PUFAs (polyunsaturated fatty acids) than MUFAs (monounsaturated fatty acids). The amino acid profile was dominated by lysine, phenylalanine, and allo-isoleucine. Both groups of fish were a good source of macro- (Na, K, Ca) and microminerals (Zn, Fe), except for selenium (Se). The two groups were not significantly different (p...
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Penelitian ini bertujuan untuk mengidentifikasi faktor internal dan eksternal yang mempengaruhi pengembangan industri patin dan menentukan strategi prioritas untuk pengembangan industri patin diProvinsi Jambi. Penelitian dilaksanakan pada... more
Penelitian ini bertujuan untuk mengidentifikasi faktor internal dan eksternal yang mempengaruhi pengembangan industri patin dan menentukan strategi prioritas untuk pengembangan industri patin diProvinsi Jambi. Penelitian dilaksanakan pada Bulan November 2018 hingga April 2019 bertempat di Provinsi Jambi (studi kasus di Kabupaten Muaro Jambi), dilakukan dengan metode SWOT (Strengths Weaknesses Opportunities and Threats) dan AHP (Analytical Hierarchy Process). Teknik pengumpulan data meliputi survei, observasi dan wawancara. Cakupan dalam penelitian ini mulai dari sektor budi daya, sektor pengolahan hingga pemasaran. Responden dalam penelitian ini adalah pelaku usaha, pembuat kebijakan (pemerintah daerah dan pusat), serta pakar (akademisi dan peneliti). Hasil identifikasi menunjukkan bahwa faktor internal dan eksternal yang mempengaruhi pengembangan industri patin pada aspek budi daya diantaranya status kepemilikan lahan, ketersediaan modal, tersedianya tenaga penyuluh perikanan, tin...
Peptones from fish waste has been widely studied, however information about its shelf life is stilllimited. This study aims to test the storability of dried peptone from tuna and shrimp waste produced through hydrolysis using alcalase... more
Peptones from fish waste has been widely studied, however information about its shelf life is stilllimited. This study aims to test the storability of dried peptone from tuna and shrimp waste produced through hydrolysis using alcalase enzyme. Peptone powders were packed in HDPE plastic bottles and plastic coated aluminum foil, stored at room temperature, and periodically observed in quality (moisture content, aw, color and appearance). A test was also performed on their ability to support the growth of Staphylococcus aureusbacteria; all were compared to commercial peptone (Difco). Shrimp waste peptone had the highest moisture, ash calcium contents, while tuna peptone has the highest fat content. During five month storage at ambient temperature, all peptones experienced a slight decrease in quality. Aluminum foil performed better than HDPE bottles as a packaging material for peptones, i.e., able to maintain the moisture content, water activity, and appearance. Although the ability to...
Telah dilakukan karakterisasi enzim transglutaminase mikroba (MTGase) yang diproduksi dari Streptoverticillium ladakanumdengan menggunakan media yang mengandung limbah cair tahu dan hidrolisat tepung tapioka. Enzim MTGase yang... more
Telah dilakukan karakterisasi enzim transglutaminase mikroba (MTGase) yang diproduksi dari Streptoverticillium ladakanumdengan menggunakan media yang mengandung limbah cair tahu dan hidrolisat tepung tapioka. Enzim MTGase yang dikarakteris as i merupakan enzim kasar yang telah dipekatkan menggunakan ultrafiltrasi dan dikeringbekukan. Enzim ini kemudian diaplikasikan pada daging lumat ikan mata goyang (Priacanthus macracanthus) lalu diamati sifat fisik (tekstur) produk restrukturisasi yang dihasilkan. Sebagai pembanding, dilakukan aplikasi TGase komersial (KTGase) pada daging lumat yang sama. Penambahan TGase dilakukan dengan 2 cara, yaitu: (1) bersama-sama dengan garam NaCl 1%, (2) bersama-sama dengan garam NaCl 1% dan sodium kaseinat 1%. Sebagai control adalah daging lumat ditambah garam N aCl 1% (tanpa penambahan enzim TGase). Hasil penelitian menunjukkan bahwa MTGase dari S. ladakanumbekerja optimum pada pH 8 dan suhu 55°C. Aktivitas enzim ini relatif tidak terpengaruh oleh ada...
The viscera are usually wasted in the processing of cowtail ray, although they may contain many valuable substances such as proteolytic enzymes. This study investigates the possibility of using these enzymes to produce fish protein... more
The viscera are usually wasted in the processing of cowtail ray, although they may contain many valuable substances such as proteolytic enzymes. This study investigates the possibility of using these enzymes to produce fish protein hydrolysates
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Fractionation of proteases of cowtail ray (Tfygort, st;plwn) viscera using polyacrylic acids has been investigated.
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Untuk mencari alternatif pepton impor, telah dilakukan penelitian pembuatan pepton dari limbah ikan. Pepton limbah ikan cair diperoleh dengan autolisis perut pari menggunakan asam klorida 2o/o (b/v) dan hidrolisis kepala tuna menggunakan... more
Untuk mencari alternatif pepton impor, telah dilakukan penelitian pembuatan pepton dari limbah ikan. Pepton limbah ikan cair diperoleh dengan autolisis perut pari menggunakan asam klorida 2o/o (b/v) dan hidrolisis kepala tuna menggunakan ensim alkalase.
Isolasi dan karakterisasi parsial kolagen dari teripang gamma (Stichopus variegatus) telah dilakukan. Isolasi dilakukan melalui tiga tahap yaitu preparasi, ekstraksi dan isolasi. Tahap preparasi meliputi tahapan penyiangan, pencucian dan... more
Isolasi dan karakterisasi parsial kolagen dari teripang gamma (Stichopus variegatus) telah dilakukan. Isolasi dilakukan melalui tiga tahap yaitu preparasi, ekstraksi dan isolasi. Tahap preparasi meliputi tahapan penyiangan, pencucian dan perendaman dalam akuades, dalam alkohol, dalam larutan Tris-HCl dan etilenadiaminatetraasetat (EDTA) dan perendaman dalam larutan natrium hidroksida (NaOH). Ekstraksi dilakukan dengan perendaman asam asetat 0,5M. Isolasi dilakukan dengan cara pengendapan menggunakan NaCl dilanjutkan proses dialisis. Isolat kolagen teripang gamma yang diperoleh memiliki rendemen sebesar 16,40% (bobot kering), nilai pH 6,08 dan derajat putih 77,02%. Gugus fungsi kolagen terdiri dari amida A (3412 cm-1), B (2929 cm-1), I (1654 cm-1), II (1554 cm-1), dan III (1239 cm-1). Asam amino utama penyusun kolagen yaitu glisin, prolin, dan alanin, masing-masing sebesar 16,88%; 6,71%; dan 6,42%. Kolagen yang dihasilkan merupakan kolagen tipe I, diduga terdiri dari 3 rantai a1 yan...
To manage with the Indonesian vision as the biggest producer of fisheries in 2015, capture,aquaculture, and post-harvest fisheries should be ready to operate at large or business scale.Consequently, industrialization should be promoted... more
To manage with the Indonesian vision as the biggest producer of fisheries in 2015, capture,aquaculture, and post-harvest fisheries should be ready to operate at large or business scale.Consequently, industrialization should be promoted since this is the most appropriate way tomanage fisheries in business manner. Though it has a positive goal to maximize the utilization offisheries resource for the improvement of economic and prosperity of Indonesian people, however,industrialization undoubtedly retains a negative impact in terms of threats on the sustainability offishery and other natural resources. This article summarises review and analysis aiming to developan ideal model in order to strengthen the fisheries industrialization in Indonesia. Based on previousweaknesses, the ideal model is the one that reflects an inclusive and holistic manner, suitable forvarious characteristics of industries in each typical areas and people of Indonesia, with the emphasison competitive advantage, u...
In the processing of cowtail ray (Trygon sephen) in Indonesia, viscera (up to 20% body weight) is wasted together with the head, frame and skin. A series of studies have been carried out to investigate the utilization of the viscera, and... more
In the processing of cowtail ray (Trygon sephen) in Indonesia, viscera (up to 20% body weight) is wasted together with the head, frame and skin. A series of studies have been carried out to investigate the utilization of the viscera, and the present paper reports the conversion of the viscera into microbiological peptones. Ensilation using 3% (v/w) mixture of propionic
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Peptones from fish waste has been widely studied, however information about its shelf life is stilllimited. This study aims to test the storability of dried peptone from tuna and shrimp waste produced through hydrolysis using alcalase... more
Peptones from fish waste has been widely studied, however information about its shelf life is stilllimited. This study aims to test the storability of dried peptone from tuna and shrimp waste produced through hydrolysis using alcalase enzyme. Peptone powders were packed in HDPE plastic bottles and plastic coated aluminum foil, stored at room temperature, and periodically observed in quality (moisture content, aw, color and appearance). A test was also performed on their ability to support the growth of Staphylococcus aureusbacteria; all were compared to commercial peptone (Difco). Shrimp waste peptone had the highest moisture, ash calcium contents, while tuna peptone has the highest fat content. During five month storage at ambient temperature, all peptones experienced a slight decrease in quality. Aluminum foil performed better than HDPE bottles as a packaging material for peptones, i.e., able to maintain the moisture content, water activity, and appearance. Although the ability to...
