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Diethyl hexyl phthalate (DEHP) is an endocrine disruptor, it influences various organ systems in human beings and experimental animals. DEHP reduced the serum testosterone and increased the blood glucose, estradiol, T(3) and T(4) in rats.... more
Diethyl hexyl phthalate (DEHP) is an endocrine disruptor, it influences various organ systems in human beings and experimental animals. DEHP reduced the serum testosterone and increased the blood glucose, estradiol, T(3) and T(4) in rats. However, the effect of DEHP on insulin signaling and glucose oxidation in skeletal muscle is not known. Adult male albino rats were divided into four groups: Group I: Control; Groups II and III: DEHP treated (dissolved in olive oil at a dose of 10 and 100mg/kg body weight, respectively, once daily through gastric intubation for 30 days); and Group IV: DEHP (100mg/kg body weight) plus vitamins E (50mg/kg body weight) and C (100mg/kg body weight) dissolved in olive oil and distilled water, respectively, once daily through gastric intubation for 30 days. On completion of treatment, animals were euthanized and perfused (whole body); gastrocnemius muscle was dissected out and subjected to assessment of various parameters. DEHP treatment increased the H(2)O(2), hydroxyl radical levels and lipid peroxidation which disrupt the membrane integrity and insulin receptor. DEHP impaired the insulin signal transduction, glucose uptake and oxidation through decreased expression of plasma membrane GLUT4, which may partly be responsible for the elevation of fasting blood glucose level. The present study suggests that DEHP exposure affects glucose oxidation in skeletal muscle and is mediated through enhanced lipid peroxidation, impaired insulin signaling and GLUT4 expression in plasma membrane. Antioxidant vitamins (C and E) have a protective role against the adverse effect of DEHP.
Research Interests: Endocrinology, Chemistry, Antioxidants, Internal Medicine, Blood Glucose, and 15 moreInsulin, Animals, Insulin signaling, Ascorbic Acid, Distilled Water, Age Factors, Body Weight, Insulin Receptor, Glucose Uptake, GLUT, Fasting Blood Glucose, Control Group, Adverse effect, Albino Rat, and Hydroxyl Radical
Research Interests: Endocrinology, Biology, Medicine, Insulin Resistance, Internal Medicine, and 15 moreBlood Glucose, Insulin, Reactive Oxygen Species, Aldosterone, Animals, Male, Phosphorylation, Lipid peroxidation, Rats, Insulin Receptor, Glucose Intolerance, Glycogen, GLUT, Biochemistry and cell biology, and Receptor Insulin
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Abstract Both organ systems, ranging from isolated skin infections to life-threatening systemic infections are implicated in the gram-positive bacterium Staphylococcus aureus, responsible for a wide range of human diseases. Filamentous... more
Abstract Both organ systems, ranging from isolated skin infections to life-threatening systemic infections are implicated in the gram-positive bacterium Staphylococcus aureus, responsible for a wide range of human diseases. Filamentous temperature-sensitive protein Z (FtsZ) is a protein encoded by the FtsZ gene that assembles into a Z-ring at the potential site of the septum of bacterial cell division. Structurally, FtsZ is a eukaryotic tubulin homolog that has a low resemblance in sequence; that makes it possible to acquire FtsZ inhibitors without impacting the division of eukaryotic cells. Hence, in our research, we tried to test compounds from the Andrographis paniculata plant using molecular docking and dynamic simulations as an efficient inhibitor of the S. aureus FtsZ protein. We have picked Andrographolide, Neoandrographolide, Andrograpanin, and 14-deoxyandrographolide as the best inhibitor for the FtsZ protein based on the docking results. In terms of docking score and hydrogen bond interaction, Andrographolide exhibited very good results relative to all other compounds. So the docked Andrographolide-FtsZ protein complex has been further analysed through simulation of molecular dynamics. Results of MD simulations indicate that the compound was excellent at linking the target protein and preserving firm connexions throughout the protein backbone structures, creating very little negligible disruption. Finally, the in vitro antimicrobial activity findings of these four compounds also showed that selected compounds may serve as a potent S.aureus inhibitor.
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Bisphenol-A (BPA) has been classified as an endocrine disruptor which disrupts normal cell function by acting as an estrogen agonist. Environmentally relevant doses of the Bisphenol-A have profound effects on rat endocrine pancreas, an... more
Bisphenol-A (BPA) has been classified as an endocrine disruptor which disrupts normal cell function by acting as an estrogen agonist. Environmentally relevant doses of the Bisphenol-A have profound effects on rat endocrine pancreas, an essential organ involved in glucose homeostasis. Bisphenol-A acts on insulin releasing β-cells whereby it increases the pancreatic insulin content and secretion and also favours post prandial hyperinsulinemia and insulin resistance in male mice. Liver plays a central role in the control of glucose production and regulation of insulin secretion. It is one of the primary organs that are initially confronted by damage from toxic substances, xenobiotics and environmental hormones. The present study was designed to assess the effect of Bisphenol-A on insulin signal transduction and glucose oxidation in liver of adult male albino rat. Wistar strain albino rats were selected and divided into three groups, Group-I: Control, Group-II: 20 mg BPA treated, Group-III: 200 mg BPA treated. The IR (insulin receptor) and Akt (PKB: protein kinase B) mRNA and protein showed a decreased expression pattern in the high dose group. Eventhough there was an increase in serum insulin and a decrease in serum testosterone levels in the high dose group, the fasting blood glucose level remained unaltered. Glucose oxidation and glycogen content were found to be decreased in both high and low dose treated groups. Results of this study suggest that Bisphenol-A treatment impairs hepatic glucose oxidation and glycogen content through defective insulin signal transduction.
Research Interests: Endocrinology, Biology, Medicine, Insulin Resistance, Internal Medicine, and 15 moreGlucose, Blood Glucose, Insulin, Liver, Hyperinsulinemia, Endocrine disruptors, Animals, Male, Phenols, Endocrine Disruptors, Glucose Homeostasis, Glycogen, ENVIRONMENTAL SCIENCE AND MANAGEMENT, Cell Membrane, and Pharmacology and pharmaceutical sciences
Adipose tissue is the primary site of storage for excess energy as triglyceride and it helps in synthesizing a number of biologically active compounds that regulate metabolic homeostasis. Consumption of high dietary fat increases stored... more
Adipose tissue is the primary site of storage for excess energy as triglyceride and it helps in synthesizing a number of biologically active compounds that regulate metabolic homeostasis. Consumption of high dietary fat increases stored fat mass and is considered as a main risk factor for metabolic diseases. Beta-sitosterol (β-sitosterol) is a plant sterol. It has the similar chemical structure like cholesterol. Clinical and experimental studies have shown that β-sitosterol has anti-diabetic, hypolipidemic, anti-cancer, anti-arthritic and hepatoprotective role. However, effect of β-sitosterol on insulin signaling molecules and glucose oxidation has not been explored. Hence in the present study we aimed to discover the protective role of β-sitosterol on the expression of insulin signaling molecules in the adipose tissue of high-fat diet and sucrose-induced type-2 diabetic experimental rats. Effect dose of β-sitosterol (20mg/kg b.wt, orally for 30 days) was given to high fat diet and sucrose-induced type-2 diabetic rats to study its anti-diabetic activity. Results of the study showed that the treatment with β-sitosterol to diabetes-induced rats normalized the altered levels of blood glucose, serum insulin and testosterone, lipid profile, oxidative stress markers, antioxidant enzymes, insulin receptor (IR) and glucose transporter 4 (GLUT4) proteins. Our present findings indicate that β-sitosterol improves glycemic control through activation of IR and GLUT4 in the adipose tissue of high fat and sucrose-induced type-2 diabetic rats. Insilico analysis also coincides with invivo results. Hence it is very clear that β-sitosterol can act as potent antidiabetic agent.
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Glyphosate, an endocrine disruptor, has an adverse impact on human health through food and also has the potential to produce reactive oxygen species (ROS), which can lead to metabolic diseases. Glyphosate consumption from food has been... more
Glyphosate, an endocrine disruptor, has an adverse impact on human health through food and also has the potential to produce reactive oxygen species (ROS), which can lead to metabolic diseases. Glyphosate consumption from food has been shown to have a substantial part in insulin resistance, making it a severe concern to those with type 2 diabetes (T2DM). However, minimal evidence exists on how glyphosate impacts insulin-mediated glucose oxidation in the liver. Hence the current study was performed to explore the potential of glyphosate toxicity on insulin signaling in the liver of experimental animals. For 16 weeks, male albino Wistar rats were given 50 mg, 100 mg and 250 mg/kg b. wt. of glyphosate orally. In the current study, glyphosate exposure group was linked to a rise in fasting sugar and insulin as well as a drop in serum testosterone. At the same time, in a dose dependent fashion, glyphosate exposure showed alternations in glucose metabolic enzymes. Glyphosate exposure resul...
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Type-2 diabetes mellitus (T2DM), the leading global health burden of this century majorly develops due to obesity and hyperglycemia-induced oxidative stress in skeletal muscles. Hence, developing novel drugs that ameliorate these... more
Type-2 diabetes mellitus (T2DM), the leading global health burden of this century majorly develops due to obesity and hyperglycemia-induced oxidative stress in skeletal muscles. Hence, developing novel drugs that ameliorate these pathological events is an immediate priority. The study was designed to analyze the possible role of Stevioside, a characteristic sugar from leaves of Stevia rebaudiana (Bertoni) on insulin signaling molecules in gastrocnemius muscle of obesity and hyperglycemia-induced T2DM rats. Adult male Wistar rats rendered diabetic by administration of high fat diet (HFD) and sucrose for 60 days were orally administered with SIT (20 mg/kg/day) for 45 days. Various parameters were estimated including fasting blood glucose (FBG), serum lipid profile, oxidative stress markers, antioxidant enzymes and expression of insulin signaling molecules in diabetic gastrocnemius muscle. Stevioside treatment improved glucose and insulin tolerances in diabetic rats and restored their ...
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Cornulin (CRNN) is linked with tumour progression. Therefore, it is of interest to document data on the molecular modeling of cornulin (CRNN) for docking with phytocompounds (Pyrazinamide, Anisotine, Vasicinone, Vasicoline) from Justicia... more
Cornulin (CRNN) is linked with tumour progression. Therefore, it is of interest to document data on the molecular modeling of cornulin (CRNN) for docking with phytocompounds (Pyrazinamide, Anisotine, Vasicinone, Vasicoline) from Justicia adhatoda L. Thus, we document the optimal binding features of these compounds with the cornulin model for further consideration
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Cyclooxygenase-2 (COX-2) is liked with breast cancer. Therefore, it is of interest to design and develop new yet effective compounds against COX-2 from medicinal plants such as the natural alkaloid compounds. We document the optimal... more
Cyclooxygenase-2 (COX-2) is liked with breast cancer. Therefore, it is of interest to design and develop new yet effective compounds against COX-2 from medicinal plants such as the natural alkaloid compounds. We document the optimal binding features of aristolochicacid with COX-2 protein for further consideration.
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Regardless of socioeconomic or demographic background, the prevalence of type 2 diabetes mellitus, which affects more than half a billion people worldwide, has been steadily increasing over time. The health, emotional, sociological, and... more
Regardless of socioeconomic or demographic background, the prevalence of type 2 diabetes mellitus, which affects more than half a billion people worldwide, has been steadily increasing over time. The health, emotional, sociological, and economic well-being of people would suffer if this number is not successfully handled. The liver is one of the key organs accountable for sustaining metabolic balance. Elevated levels of reactive oxygen species inhibit the recruitment and activation of IRS-1, IRS-2, and PI3K-Akt downstream signaling cascade. These signaling mechanisms reduce hepatic glucose absorption and glycogenesis while increasing hepatic glucose output and glycogenolysis. In our work, an analysis of the molecular mechanism of Carica papaya in mitigating hepatic insulin resistance in vivo and in silico was carried out. The gluconeogenic enzymes, glycolytic enzymes, hepatic glycogen tissue concentration, oxidative stress markers, enzymatic antioxidants, protein expression of IRS-2...
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Driver distraction is considered as major factors in most of the traffic accidents. Driving errors may arise due to the distraction of the drivers. The aim of this paper is to analyze the EEG signals to detect distractive driving. Data... more
Driver distraction is considered as major factors in most of the traffic accidents. Driving errors may arise due to the distraction of the drivers. The aim of this paper is to analyze the EEG signals to detect distractive driving. Data from 10 different subjects were obtained and categorised into different frequency bands. Distractive driving is related with Theta band, so the Theta frequency band were decomposed using Discrete Wavelet Transform (DWT) and 17 different features were extracted. By enabling Principle Component Analysis (PCA) the accuracy rate was found for Cognitive Distraction and Visual Distraction using different machine learning algorithms. K Nearest Neighbour (KNN) performed well when compared to other machine learning algorithms with a better accuracy rate of 71.1%.
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More than 20% of road accidents are due to driver fatigue and considered to be major inference for transportation safety. This study aims to analyze fatigue state from the EEG signals of the drivers while performing a simulated driving... more
More than 20% of road accidents are due to driver fatigue and considered to be major inference for transportation safety. This study aims to analyze fatigue state from the EEG signals of the drivers while performing a simulated driving task. Ten healthy participants involved in this study at three different timings. EEG signals were recorded continuously during monotonous driving in the simulator. Based on the video analysis the fatigue state and normal state related EEG signals were collected and decomposed using discrete wavelet transform. In this work the alpha and theta sub bands were considered for analysing the fatigue state of drivers. Features were extracted and classified using machine learning algorithm such as SVM, KNN and Ensemble. The result shows an accuracy of 97.8% in occipital region (O1 in alpha and O2 in theta) for classifying the fatigue state.
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Road accidents are major issue that creates problem in the society. To overcome the issue, driver behavior and the driving environment has to be monitored regularly and alert the driver before any accident may occur. This could be... more
Road accidents are major issue that creates problem in the society. To overcome the issue, driver behavior and the driving environment has to be monitored regularly and alert the driver before any accident may occur. This could be possible only on analyzing four measures: subjective, behavioral, physiological and vehicle-based. These combined hybrid measures can provide better accuracy on determining various states of driver like normal, drowsy, cognitive and visual inattention. In hybrid measures, physiological measure plays a major role by increasing the accuracy, specificity, sensitivity of the driver state detection. Physiological measures are the signals recorded from various parts of the human body using biosensors to check the internal condition of the driver while driving. These signals can be Electroencephalogram (EEG), Electrocardiogram (ECG), Electro-oculogram (EOG) and Electromyogram (EMG). EEG signals are the brain activity data which corresponds to EEG wave band determines the physical state of the driver. Most researchers have worked on EEG for better accuracy in driver state detection. This paper is one such review on various measures in determining driver states while driving.
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Beta-catenin is linked with colorectal cancer (CRC). Therefore, it is of interest to design and develop novel compounds to combat CRC. Hence, we document compounds (chlorogenic acid, gallic acid, protocatechuic acid, quercetin and... more
Beta-catenin is linked with colorectal cancer (CRC). Therefore, it is of interest to design and develop novel compounds to combat CRC. Hence, we document compounds (chlorogenic acid, gallic acid, protocatechuic acid, quercetin and vanillic acid) from Lycopersicon esculentum with optimal binding features for further consideration.
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Chlamydophila pneumoniae is an intracellular pathogen accountable for various acute respiratory infections. C. pneumoniae has a gene cluster which encodes a putative outer membrane porin (aaxA), arginine decarboxylase (CPn1032 or aaxB)... more
Chlamydophila pneumoniae is an intracellular pathogen accountable for various acute respiratory infections. C. pneumoniae has a gene cluster which encodes a putative outer membrane porin (aaxA), arginine decarboxylase (CPn1032 or aaxB) and a putative cytoplasmic membrane transporter (CPn1031 or aaxC). Therefore, it is of interest to document a molecular protein model of porin AaxA from Chlamydia pneumonia to gain structure to functional insight on the protein.
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A siddha polyherbal preparation consisting of 5 medicinal plants, namely, Asparagus racemosus, Emblica officinalis, Salacia oblonga, Syzygium aromaticum, and Tinospora cordifolia, in equal ratio, was formulated to examine the molecular... more
A siddha polyherbal preparation consisting of 5 medicinal plants, namely, Asparagus racemosus, Emblica officinalis, Salacia oblonga, Syzygium aromaticum, and Tinospora cordifolia, in equal ratio, was formulated to examine the molecular mechanism by which it exhibits antidiabetic effects in the liver of high-fat and fructose-induced type 2 diabetic rats. The polyherbal preparation treated type 2 diabetic rats showed an increase in insulin receptor, Akt, and glucose transporter2 mRNA levels compared with diabetic rats. Insulin receptor, insulin receptor substrate-2, Akt, phosphorylated Akt substrate of 160kDaThreonine642, α-Actinin-4, β-arrestin-2, and glucose transporter2 proteins were also markedly decreased in diabetic rats, whereas the polyherbal preparation treatment significantly improved the expression of these proteins more than that of metformin-treated diabetic rats. The expression pattern of insulin signaling molecules analyzed in the present study signifies the therapeutic...
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Diethyl hexyl phthalate (DEHP) is a plasticizer, commonly used in a variety of products, including lubricants, perfumes, hairsprays and cosmetics, construction materials, wood finishers, adhesives, floorings and paints. DEHP is an... more
Diethyl hexyl phthalate (DEHP) is a plasticizer, commonly used in a variety of products, including lubricants, perfumes, hairsprays and cosmetics, construction materials, wood finishers, adhesives, floorings and paints. DEHP is an endocrine disruptor and it has a continuum of influence on various organ systems in human beings and experimental animals. However, specific effects of DEHP on insulin signaling in adipose tissue are not known. Adult male albino rats of Wistar strain were divided into four groups. Control, DEHP treated (dissolved in olive oil at a dose of 10, and 100 mg/kg body weight, respectively, once daily through gastric intubations for 30 days) and DEHP + vitamin E (50 mg/kg body weight) and C (100 mg/kg body weight) dissolved in olive oil and distilled water, respectively, once daily through gastric intubations for 30 days. After the completion of treatment, adipose tissue was dissected out to assess various parameters. DEHP treatment escalated H(2)O(2) and hydroxyl radical levels as well as lipid peroxidation in the adipose tissue. DEHP impaired the expression of insulin signaling molecules and their phosphorelay pathways leading to diminish plasma membrane GLUT4 level and thus decreased glucose uptake and oxidation. Blood glucose level was elevated as a result of these changes. Supplementation of vitamins (C & E) prevented the DEHP-induced changes. It is concluded that DEHP-induced ROS and lipid peroxidation disrupts the insulin signal transduction in adipose tissue and favors glucose intolerance. Antioxidant vitamins have a protective role against the adverse effect of DEHP.
Research Interests: Endocrinology, Chemistry, Environmental Toxicology, Cell Signaling, Insulin Resistance, and 15 moreAntioxidants, Adipose tissue, Internal Medicine, Glucose, Blood Glucose, Insulin, Diabetes mellitus, Animals, Antioxidant, Diabetes and Insulin Resistance, Hydrogen Peroxide, Ascorbic Acid, Insulin Receptor, Glucose Intolerance, and Biochemistry and cell biology
Aroclor 1254 is the commercial mixture of highly toxic environmental pollutant, polychlorinated biphenyls (PCBs). Being immensely durable, it is extensively used and widely distributed. Studies show that Aroclor 1254 causes a variety of... more
Aroclor 1254 is the commercial mixture of highly toxic environmental pollutant, polychlorinated biphenyls (PCBs). Being immensely durable, it is extensively used and widely distributed. Studies show that Aroclor 1254 causes a variety of adverse health effects through free radical generation. The present investigation was designed to check the effect of Aroclor 1254 on the glucose transporter protein, GLUT4, which plays a key role in glucose homeostasis. The protective role of lycopene against the adverse effect of Aroclor 1254 was also tested. Group 1 rats received corn oil as vehicle and served as control. Groups 2, 3, and 4 were administered with Aroclor 1254 [2 mg kg(-1) body weight (b.w.) day(-1)] intraperitoneally for 30 days. Groups 3 and 4 received lycopene (2 and 4 mg kg(-1) b.w. day(-1), respectively) orally in addition to Aroclor 1254. After 30 days, animals were euthanized and the skeletal muscles were dissected to determine the following parameters: GLUT4 messenger RNA (mRNA), GLUT4 protein (both plasma membrane and cytosolic fractions), and (14)C-2-deoxyglucose uptake. Though there was no change in GLUT4 mRNA and fasting plasma glucose levels, Aroclor 1254 significantly decreased the GLUT4 protein level in both the subcellular fractions of the gracilis and triceps muscles. Most important, (14)C-2-deoxyglucose uptake showed a significant decrease in Aroclor 1254 alone treated rats, and Aroclor 1254 plus 4 mg lycopene supplementation treatment maintained the same at par with control. Thus, Aroclor 1254 has adverse effects on GLUT4 translocation and (14)C-2-deoxyglucose uptake, and lycopene administered along with Aroclor 1254 has a protective role over it.
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The prevalence of obesity in contemporary society has brought attention to how serious it is all around the world. Obesity, a proinflammatory condition defined by hypertrophied adipocytes and immune cells that reside in adipose tissue, is... more
The prevalence of obesity in contemporary society has brought attention to how serious it is all around the world. Obesity, a proinflammatory condition defined by hypertrophied adipocytes and immune cells that reside in adipose tissue, is characterized by elevated circulating levels of proinflammatory cytokines. The pro-inflammatory mediators trigger a number of inflammatory pathways and affect the phosphorylation of a number of insulin-signaling pathways in peripheral tissues. In this work, we pointed the outcome of the leaves of Carica papaya (C. papaya) on the inflammatory molecules by in vivo and in silico analysis in order to prove its mechanisms of action. Adipocytokines, antioxidant enzymes, gene and protein expression of pro-inflammatory signaling molecules (mTOR, TNF-α, IL-1β, IL-6 and IKKβ) by q-RT-PCR and immunohistochemistry, as well as histopathological analysis, in adipose tissues were carried out. C. papaya reinstated the levels of adipocytokines, antioxidant enzymes ...
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In the management of type 2 diabetes, oral antidiabetic drugs have several side effects, which in turn have led the pharmaceutical industry to search for good therapeutic, non-toxic and reliable drugs. Carica papaya (C. papaya) is one of... more
In the management of type 2 diabetes, oral antidiabetic drugs have several side effects, which in turn have led the pharmaceutical industry to search for good therapeutic, non-toxic and reliable drugs. Carica papaya (C. papaya) is one of several plants in nature that have been found to possess anti-diabetic properties. Despite studies being focused on the antidiabetic activity of C. papaya, the molecular mechanism against high fat diet induced insulin resistance is yet to be identified. The role of C. papaya was evaluated on insulin signaling molecules, such as the insulin receptor (IR) and glucose transporter-4 (GLUT4) in high fat, diet-streptozotocin induced type 2 diabetic rats, and analyzed the bioactive compounds of C. papaya against IR and GLUT4 via molecular docking and dynamics. The ethanolic extract of C. papaya leaves (600 mg/kg of body weight) was given daily to male wistar rats for 45 days and we observed the various biochemical parameters, gene expression analysis and h...
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Despite rigorous endeavors, existing attempts to handle type 2 diabetes (T2DM) are still a long way off, as a substantial number of patients do not meet therapeutic targets. Insulin resistance in skeletal muscle is discerned as a... more
Despite rigorous endeavors, existing attempts to handle type 2 diabetes (T2DM) are still a long way off, as a substantial number of patients do not meet therapeutic targets. Insulin resistance in skeletal muscle is discerned as a forerunner in the pathogenesis of T2DM and can be detected years before its progress. Studies have revealed the antidiabetic properties of Carica papaya (C. papaya), but its molecular mechanism on insulin receptor substrate-1 (IRS-1)/Akt signaling mechanisms is not yet known. The present study aimed to evaluate the role of C. papaya on IRS1 and Akt in high-fat-diet–streptozotocin-induced type 2 diabetic rats and also to analyze the bioactive compounds of C. papaya against IRS-1 and Akt via in silico analysis. Ethanolic extract of the leaves of C. papaya (600 mg/kg of body weight) was given daily for 45 days postinduction of T2DM up to the end of the study. Gluconeogenic enzymes, glycolytic enzymes, gene expression, and immunohistochemical analysis of IRS-1 ...
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Aim: The aim of this study is to assess the levels of 8-hydroxy-2 deoxyguanosine as a salivary biomarker for periodontitis with or without diabetes mellitus. Study Design: Cross-sectional study Materials and Methods: 30 patients were... more
Aim: The aim of this study is to assess the levels of 8-hydroxy-2 deoxyguanosine as a salivary biomarker for periodontitis with or without diabetes mellitus. Study Design: Cross-sectional study Materials and Methods: 30 patients were included in the study and were categorised into three groups: periodontal health (Group a), patients with periodontitis without diabetes mellitus (Group b) and patients with periodontitis only (Group c). Unstimulated salivary samples were taken. The OHdG concentration was evaluated using Sandwich-enzyme linked immunosorbent assay by commercially available human OHdG 96 well ELISA kit. The data were statistically analysed by One-Way -ANOVA. The Newman-Keuls multiple comparison test was used to test the significance at the levels of p<0.05. Results: Salivary OHdG concentration in periodontal health, periodontitis with diabetes mellitus (P+DM), Periodontitis only (P) were analysed. Salivary OHdG concentration was found to be significantly higher (p<...
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Aim: The present study aimed to analyse the cytotoxicity of zinc oxide nanoparticles (ZnO NPs) that are biosynthesized using extract of Abies webbiana Methodology: Zinc oxide nanoparticles were synthesized by green approach using A.... more
Aim: The present study aimed to analyse the cytotoxicity of zinc oxide nanoparticles (ZnO NPs) that are biosynthesized using extract of Abies webbiana Methodology: Zinc oxide nanoparticles were synthesized by green approach using A. webbiana and were characterized by UV- vis spectroscopy. The powdered A. webbiana mediated ZnO NPs were subjected to cytotoxic tests for different concentrations by brine shrimp lethality assay. Results: ZnO NPs synthesized from A. webbiana showed reduced toxicity at lower concentrations. After 48 hours, it was found that at a minimal concentration of 5 µl, 90% of the nauplii and in concentration of 10 µl and 20 µl, 80% of the nauplii were alive. At a concentration of 40 µl and 80 µl, 70% of the nauplii were alive. Whereas the control showed 100% of the nauplii to be alive. Conclusion: Various nanoformulations of A. webbiana mediated ZnO NPs can be developed at optimal concentrations for therapeutics that are safe and economical.
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Diabetes mellitus has become a troublesome and increasingly widespread condition. Treatment strategies for diabetes prevention in high-risk as well as in affected individuals are largely attributed to improvements in lifestyle and dietary... more
Diabetes mellitus has become a troublesome and increasingly widespread condition. Treatment strategies for diabetes prevention in high-risk as well as in affected individuals are largely attributed to improvements in lifestyle and dietary control. Therefore, it is important to understand the nutritional factors to be used in dietary intervention. A decreased risk of diabetes is associated with daily intake of millet-based foods. Pearl millet is a highly nutritious grain, nutritionally comparable and even superior in calories, protein, vitamins, and minerals to other large cereals, although its intake is confined to lower income segments of society. Pearl millet contains phenolic compounds which possess antidiabetic activity. Thus, it can be used to prepare a variety of food products for diabetes mellitus. Moreover, it also has many health benefits, including combating diabetes mellitus, cancer, cardiovascular conditions, decreasing tumour occurrence, lowering blood pressure, heart d...