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Ioannis  Leonardos

    Ioannis Leonardos

    ΠΑΡΑΔΟΤΕΟ Α5 : ΤΕΚΜΗΡΙΩΜΕΝΗ ΠΡΟΤΑΣΗ ΠΡΟΓΡΑΜΜΑΤΙΣΜΟΥ ΕΡΓΑΣΙΩΝ ΠΕΔΙΟΥ ΑΝΑΔΟΧΟΣ ΚΟΙΝΟΠΡΑΞΙΑ ΑΝΑΓΝΟΠΟΥΛΟΣ ΝΙΚΟΛΑΟΣ -ΕΠΕΜ Α.Ε. -ΚΩΝΣΤΑΝΤΙΝΙΔΗΣ ΗΛΙΑΣ Ευρωπαϊκή Ένωση Ευρωπαϊκό Ταμείο Περιφερειακής Ανάπτυξης (ΕΤΠΑ). Με τη συγχρηματοδότηση της... more
    ΠΑΡΑΔΟΤΕΟ Α5 : ΤΕΚΜΗΡΙΩΜΕΝΗ ΠΡΟΤΑΣΗ ΠΡΟΓΡΑΜΜΑΤΙΣΜΟΥ ΕΡΓΑΣΙΩΝ ΠΕΔΙΟΥ ΑΝΑΔΟΧΟΣ ΚΟΙΝΟΠΡΑΞΙΑ ΑΝΑΓΝΟΠΟΥΛΟΣ ΝΙΚΟΛΑΟΣ -ΕΠΕΜ Α.Ε. -ΚΩΝΣΤΑΝΤΙΝΙΔΗΣ ΗΛΙΑΣ Ευρωπαϊκή Ένωση Ευρωπαϊκό Ταμείο Περιφερειακής Ανάπτυξης (ΕΤΠΑ). Με τη συγχρηματοδότηση της Ελλάδας και της Ευρωπαϊκής Ένωσης Αθήνα, Ιούλιος 2014 «ΕΠΟΠΤΕΙΑ ΚΑΙ ΑΞΙΟΛΟΓΗΣΗ ΤΗΣ ΚΑΤΑΣΤΑΣΗΣ ΔΙΑΤΗΡΗΣΗΣ ΕΙΔΩΝ ΙΧΘΥΟΠΑΝΙΔΑΣ ΚΟΙΝΟΤΙΚΟΥ ΕΝΔΙΑΦΕΡΟΝΤΟΣ ΣΤΗΝ ΕΛΛΑΔΑ» ΚΟΙΝΟΠΡΑΞΙΑ : ΑΝΑΓΝΟΠΟΥΛΟΣ ΝΙΚΟΛΑΟΣ -ΕΠΕΜ Α.Ε.-ΚΩΝΣΤΑΝΤΙΝΙΔΗΣ ΗΛΙΑΣ II Πίνακας περιεχομένων
    Research Interests:
    ... The first record of the decapod from Greece (Axios River) was made by Bouvier (1913). Holthuis (1961) reported the species from Lake Koronia (as Atyaephyra desmarestii desmarestii). ... Bulletin Musée Histoire Naturelle, Paris... more
    ... The first record of the decapod from Greece (Axios River) was made by Bouvier (1913). Holthuis (1961) reported the species from Lake Koronia (as Atyaephyra desmarestii desmarestii). ... Bulletin Musée Histoire Naturelle, Paris 19:65–74. Bouvier, EL. 1925. ...
    ... The first record of the decapod from Greece (Axios River) was made by Bouvier (1913). Holthuis (1961) reported the species from Lake Koronia (as Atyaephyra desmarestii desmarestii). ... Bulletin Musée Histoire Naturelle, Paris... more
    ... The first record of the decapod from Greece (Axios River) was made by Bouvier (1913). Holthuis (1961) reported the species from Lake Koronia (as Atyaephyra desmarestii desmarestii). ... Bulletin Musée Histoire Naturelle, Paris 19:65–74. Bouvier, EL. 1925. ...
    The complete series of larval staging of Hippolyte sapphica d’Udekem d’Acoz, 1993 forma A and B from Louros estuary was studied in the laboratory and described in detail for the first time. The reared larvae of H. sapphica passed through... more
    The complete series of larval staging of Hippolyte sapphica d’Udekem d’Acoz, 1993 forma A and B from Louros estuary was studied in the laboratory and described in detail for the first time. The reared larvae of H. sapphica passed through six zoeal stages and one megalopal stage. The larval monitoring completed when the individuals could be assigned clearly to form A and B via rostra formation. Under the experimental conditions, the average durations of the larval stages were as follows: three days for 1st and 2nd stages, three to four days for 3rd, 4th, 5th and 6th stages, four days for the megalopal stage and 19 to 30 days for immature form A and B individuals. Comparison of the larval morphological characters among the described material and the bibliographic data of closely related species was made and discussed. The offspring of females of the forma A includes forma A and B and the same can be said of the offspring of the forma B. This confirms that the formae A and B are indeed...
    The Mediterranean toothcarp Aphanius fasciatus (Valenciennes, 1821) is a small-sized omnivorous estuarine fish. Its diet is dominated by juveniles of shrimps (Palaemon adspersus), Isopods, Branchiopoda, Bivalvia, eggs of invertebrates,... more
    The Mediterranean toothcarp Aphanius fasciatus (Valenciennes, 1821) is a small-sized omnivorous estuarine fish. Its diet is dominated by juveniles of shrimps (Palaemon adspersus), Isopods, Branchiopoda, Bivalvia, eggs of invertebrates, mosquitoes (adults and larvae) and diatoms. An ontogenetic diet shift with an increase in mean prey size with fish length was observed. Smaller fish feed on planktonic prey (e.g. copepods, ostracods, nauplii of Artemia), while larger fish prefer larger and more benthic preys (e.g. ampipods, Bivalvia). The diet of A. fasciatus shows a high degree of seasonal variation, with a reduction in the feeding activity during the periods of adverse environmental conditions (winter and autumn). It is a well-adapted estuarine fish, its feeding mode and preferences depending on the preys that are available. Its feeding strategy is characterised by specialisation in different resource types (aquatic invertebrates and mosquitoes) and a high between-phenotype contribu...
    First report in “Monitoring and evaluation of conservation status of EU’s interest icthyofanuna species in Greece”. Defining taxonomic issues with fishes included in the Natura 2000 monitoring project. The project is a collaboration of... more
    First report in “Monitoring and evaluation of conservation status of EU’s interest icthyofanuna species in Greece”. Defining taxonomic issues with fishes included in the Natura 2000 monitoring project. The project is a collaboration of several organizations including GMEECC, Athens, JOINT VENTURE ANAGNOPOULOS NIKOLAOS – EPEM S.A. – KONSTANTINIDIS ILIAS, Athens, 20 pages (in Greek).
    A complete serial of larval staging of Atyaephyra desmarestii (Millet, 1831) sensu lato from Louros River (north-western Greece) was obtained in the laboratory and described in detail. Thirty berried females were collected and transferred... more
    A complete serial of larval staging of Atyaephyra desmarestii (Millet, 1831) sensu lato from Louros River (north-western Greece) was obtained in the laboratory and described in detail. Thirty berried females were collected and transferred to an aquarium containing water and vegetation from the sampling site. The newly hatched larvae were transferred into small sterile aquaria and mass cultured. After each moult, several larvae were photographed and preserved with their exuviae. The reared larvae of A. desmarestii sensu lato passed through six zoeal stages and one megalopal stage. At the experimental conditions, the durations of each larval stage were as follows: three days for the 1st larval stage; three to four days for 2nd larval stage; six to eight days for 3rd; seven to nine days for 4th; nine to ten days for 5th, 6th and megalopal stages. All the obtained stages were described in detail. The larval morphological characters of the described material and the larvae specimens coll...
    Morphology of native brown trout (Salmo spp.) from four major rivers of Western Greece was analyzed using 36 morphometric and 7 meristic characters in order to clarify its taxonomic status and provide insights about the life models... more
    Morphology of native brown trout (Salmo spp.) from four major rivers of Western Greece was analyzed using 36 morphometric and 7 meristic characters in order to clarify its taxonomic status and provide insights about the life models followed by these populations. Significant between- population differences were observed in both types of characters. Discriminant analysis allowed correct classification of 92.9% of all specimens. Differences in morphometric traits were related to body shape as well as to fin positioning and length. Cluster analysis carried out separately on mean morphometric and modal meristic values of populations indicated (in both cases) distinct position of the trout population from Louros River. Morphometrics of S. lourosensis suggest a spatially restricted resident model of life, in which case conservation measures should be focused on restoration and protection of the uppermost part of the stream where the species is abundant.
    ΠΑΡΑΔΟΤΕΟ Α5 : ΤΕΚΜΗΡΙΩΜΕΝΗ ΠΡΟΤΑΣΗ ΠΡΟΓΡΑΜΜΑΤΙΣΜΟΥ ΕΡΓΑΣΙΩΝ ΠΕΔΙΟΥ ΑΝΑΔΟΧΟΣ ΚΟΙΝΟΠΡΑΞΙΑ ΑΝΑΓΝΟΠΟΥΛΟΣ ΝΙΚΟΛΑΟΣ -ΕΠΕΜ Α.Ε. -ΚΩΝΣΤΑΝΤΙΝΙΔΗΣ ΗΛΙΑΣ Ευρωπαϊκή Ένωση Ευρωπαϊκό Ταμείο Περιφερειακής Ανάπτυξης (ΕΤΠΑ). Με τη συγχρηματοδότηση της... more
    ΠΑΡΑΔΟΤΕΟ Α5 : ΤΕΚΜΗΡΙΩΜΕΝΗ ΠΡΟΤΑΣΗ ΠΡΟΓΡΑΜΜΑΤΙΣΜΟΥ ΕΡΓΑΣΙΩΝ ΠΕΔΙΟΥ ΑΝΑΔΟΧΟΣ ΚΟΙΝΟΠΡΑΞΙΑ ΑΝΑΓΝΟΠΟΥΛΟΣ ΝΙΚΟΛΑΟΣ -ΕΠΕΜ Α.Ε. -ΚΩΝΣΤΑΝΤΙΝΙΔΗΣ ΗΛΙΑΣ Ευρωπαϊκή Ένωση Ευρωπαϊκό Ταμείο Περιφερειακής Ανάπτυξης (ΕΤΠΑ). Με τη συγχρηματοδότηση της Ελλάδας και της Ευρωπαϊκής Ένωσης Αθήνα, Ιούλιος 2014 «ΕΠΟΠΤΕΙΑ ΚΑΙ ΑΞΙΟΛΟΓΗΣΗ ΤΗΣ ΚΑΤΑΣΤΑΣΗΣ ΔΙΑΤΗΡΗΣΗΣ ΕΙΔΩΝ ΙΧΘΥΟΠΑΝΙΔΑΣ ΚΟΙΝΟΤΙΚΟΥ ΕΝΔΙΑΦΕΡΟΝΤΟΣ ΣΤΗΝ ΕΛΛΑΔΑ» ΚΟΙΝΟΠΡΑΞΙΑ : ΑΝΑΓΝΟΠΟΥΛΟΣ ΝΙΚΟΛΑΟΣ -ΕΠΕΜ Α.Ε.-ΚΩΝΣΤΑΝΤΙΝΙΔΗΣ ΗΛΙΑΣ II Πίνακας περιεχομένων
    This article investigates the controls on sediment phosphorus (P) speciation dynamics as a function of its fractionation into chemically defined operational pools along a river continuum. A total of 27 variables were analyzed in bed... more
    This article investigates the controls on sediment phosphorus (P) speciation dynamics as a function of its fractionation into chemically defined operational pools along a river continuum. A total of 27 variables were analyzed in bed sediment samples collected for one year from six sampling points, along a 75km river continuum (Louros River, NW Greece). Multivariate explanatory analysis of the complex experimental data matrix was performed in order to unravel the spatial pattern of P speciation. Non-parametric examinations were also applied in order to elucidate the temporal variations encountered in the speciation of P. The results suggest that inorganic P species control P bioavailability in space and time. Organic P was found to be very reactive among the various fractions thus changing its bioavailability but exhibited no temporal variation. The utility of the proposed approach in the differentiation of natural and anthropogenic P inputs and their classification to point and non-...
    The heart of the zebrafish has been used extensively to assess the cardiotoxic effect of compounds, using the frequency of heart contractions as the main index of cardiac response to drugs. In this study, the force and the frequency... more
    The heart of the zebrafish has been used extensively to assess the cardiotoxic effect of compounds, using the frequency of heart contractions as the main index of cardiac response to drugs. In this study, the force and the frequency generated by the spontaneously contracting zebrafish heart, isolated in saline, were found to be 0.87 ± 0.05 mN and 1.54 ± 0.03 Hz (n=6) respectively within the first hour of recording. Both values of force and frequency remained constant for over 8h. The advantage of prolonged vitality in the assessment of cardiovascular toxicity was shown using the well-known anticancer drug adriamycin, which has severe cardiotoxic side effects. At 10.0 μM there was a 21.05 ± 4.42% (p=0.02, n=4) decrease in the force of contraction, while the frequency was not affected after 3h treatment (p>0.05). At 50.0 and 100.0 μM there was a 33.24 ± 3.0 and 46.6 ± 4.80% irreversible decrease in force (p<0.05, n=4), while a 18.02 ± 4.07% and 16.16 ± 4.07% reversible increase was observed in the frequency (p=0.02, n=4). These contradictory positive chronotropic and negative inotropic responses indicate the strong inhibitory effect of adriamycin on ventricular cardiomyocytes and its excitatory effects on auto-rhythmical pacemaker cells. If heart frequency was the only parameter used to assess the cardiotoxic effect of adriamycin, at the above range of concentrations, this compound would have been classified as non-cardiotoxic.
    ... is an increasing body of literature on persistence and stability of lotic invertebrate communities in ... Macroinvertebrates were sorted to the lowest practicable taxonomic level. ... Multivariate analysis of variance (MANOVA) was... more
    ... is an increasing body of literature on persistence and stability of lotic invertebrate communities in ... Macroinvertebrates were sorted to the lowest practicable taxonomic level. ... Multivariate analysis of variance (MANOVA) was performed to study the possible effect of sampling station ...
    ABSTRACT Morphological patterns among species of the genus Rutilus from the southern Balkan Peninsula were analysed to identify morphological differentiation and its palaeogeographic history. Seven meristic and 65 morphometric characters,... more
    ABSTRACT Morphological patterns among species of the genus Rutilus from the southern Balkan Peninsula were analysed to identify morphological differentiation and its palaeogeographic history. Seven meristic and 65 morphometric characters, based on 20 landmarks, were studied on 711 specimens from 15 Greek lakes. Multivariate analyses demonstrated a well-supported differentiation of the western and northern from the eastern Rutilus species' populations, separated by the Pindos Mountains. In North-western Greece, there are three isolated endemic species, in different biogeographical areas (R. prespensis in the Southern Adriatic, R. panosi in the Ionian and R. ylikiensis in the Attico-Boeotia), while in the Northern Aegean, multiple non-homogeneous populations of the cosmopolitan R. rutilus can be found. This distribution is clearly in accordance with the biogeographical scenario for the distribution of freshwater fauna of the Balkan Peninsula. This study confirms differentiation of the populations of Lakes Kastoria, Volvi, Vegoritida and Doirani, and emphasizes the likely existence of cryptic species within R. rutilus populations.
    ABSTRACT Modern theories of life history evolution deal with finding links between environmental factors, demographic structure of animal populations and the optimal life history strategy. Small-sized teleost fish, occurring in fragmented... more
    ABSTRACT Modern theories of life history evolution deal with finding links between environmental factors, demographic structure of animal populations and the optimal life history strategy. Small-sized teleost fish, occurring in fragmented populations under contrasting environments, have been widely used as study models to investigate these issues. In the present study, the Mediterranean killifish Aphanius fasciatus was used to investigate the relationships between some habitat features and life history strategy. We selected four sites in the Venice lagoon inhabited by this species, exhibiting different combinations of two factors: overall adult mortality, related to intertidal water coverage and a consequent higher level of predator exposure, and the level of sediment organic matter, as indicator of habitat trophic richness. Results showed that these were the two most important factors influencing demography and life history traits in the four sites. Fish from salt marshes with high predator pressure were smaller and produced a higher number of eggs, whereas bigger fish and a lower reproductive investment were found in the two closed, not tidally influenced habitats. Habitat richness was positively related with population density, but negatively related with growth rate. In particular the synergy between high resources and low predation level was found to be important in shaping peculiar life history traits. Results were discussed in the light of the interactions between selective demographic forces acting differentially on age/size classes, such as predation, and habitat trophic richness that may represent an important energetic constraint on life history traits. The importance to link habitat productivity and morphology to demographic factors for a better understanding of the evolution of life history strategy under contrasting environments was finally suggested. open access at http://elsarticle.com/1ejh6kf
    We studied the relationships between planktonic respiration and key physicochemical parameters and total autochthonous biomass for three years in Lake Pamvotis, a shallow and eutrophic Greek lake. Planktonic community respiration was... more
    We studied the relationships between planktonic respiration and key physicochemical parameters and total autochthonous biomass for three years in Lake Pamvotis, a shallow and eutrophic Greek lake. Planktonic community respiration was correlated with only one environmental parameter, dissolved inorganic nitrogen concentration, which appeared to be the limiting nutrient. Community respiration was also related to heterotrophic bacterial abundance.
    The gynogenetic population of Prussian carp Carassius gibelio in Lake Pamvotis (north-west Greece) included a small percentage of males (2-3%). Cytogenetic analysis revealed heterogeneity of the population at the ploidy level: all females... more
    The gynogenetic population of Prussian carp Carassius gibelio in Lake Pamvotis (north-west Greece) included a small percentage of males (2-3%). Cytogenetic analysis revealed heterogeneity of the population at the ploidy level: all females were triploid (modal chromosome number 156-162 chromosomes) while the males were either triploid (modal number 156-158 chromosomes) or tetraploid (modal number 200-214 chromosomes). Morphometric analysis revealed significant differences in body shape among these three groups. The males were more slender than females, while the triploid males could be discriminated from the tetraploid by the morphology of their caudal peduncle. The males attained a significantly lower asymptotic standard length (L(S)) (25.04 cm) than females (29.75 cm) and had lower exponent b of the L(S) and mass relationship. Under laboratory conditions, intraspecific crossings of C. gibelio males with females could give viable all-female offspring; in comparison with goldfish Carassius auratus males, the fertility of the C. gibelio males was generally reduced but remained highly variable among individuals.
    Variation in three different clones of the invasive sperm-dependent, cyprinid fish Carassius gibelio were examined in Lake Pamvotis (north-west Greece). Differences between the clones were found in their proportion in the population, in... more
    Variation in three different clones of the invasive sperm-dependent, cyprinid fish Carassius gibelio were examined in Lake Pamvotis (north-west Greece). Differences between the clones were found in their proportion in the population, in their age structure, in the time of arrival to their spawning grounds and in the coefficients of the von Bertalanffy growth equation.
    ... The first record of the decapod from Greece (Axios River) was made by Bouvier (1913). Holthuis (1961) reported the species from Lake Koronia (as Atyaephyra desmarestii desmarestii). ... Bulletin Musée Histoire Naturelle, Paris... more
    ... The first record of the decapod from Greece (Axios River) was made by Bouvier (1913). Holthuis (1961) reported the species from Lake Koronia (as Atyaephyra desmarestii desmarestii). ... Bulletin Musée Histoire Naturelle, Paris 19:65–74. Bouvier, EL. 1925. ...
    ABSTRACT

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