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Fernando Hanashiro

    Fernando Hanashiro

    Although the cariostatic effects of CO2 laser on the root surface have been shown, there is scarce information regarding its effects on root secondary caries. The objective of this research was to investigate the effect of the association... more
    Although the cariostatic effects of CO2 laser on the root surface have been shown, there is scarce information regarding its effects on root secondary caries. The objective of this research was to investigate the effect of the association of CO2 laser and a fluoride dentifrice on the inhibition of secondary caries on root surfaces adjacent to composite-resin or glass-ionomer-cement restorations. Dental blocks of human roots were divided into two groups: composite resin (CR) or glass ionomer cement (GIC). Subsequently, the blocks were divided into four subgroups (n = 10): C, non-fluoride dentifrice; FD, fluoride dentifrice; L, CO2 laser with an energy density of 6.0 J cm−2 + non-fluoride dentifrice; and L + FD, CO2 laser + fluoride dentifrice. The blocks were subjected to pH cycling to simulate a high cariogenic challenge. Dental demineralization around the restorations was quantified by microhardness analysis. The results were subjected to analysis of variance (ANOVA) and the Tukey–Kramer test (p ≤ 0.05). As for mineral loss, it can be observed that all the groups that were treated with a fluoride dentifrice and laser, used alone or not, were statistically similar and superior to the RC–C group. It was concluded that CO2 laser irradiation and a fluoride dentifrice used alone or combined with each other are efficient surface treatments for preventing secondary root caries, regardless of the restorative material used.
    Although the cariostatic effects of CO2 laser on the root surface have been shown, there is scarce information regarding its effects on root secondary caries. The objective of this research was to investigate the effect of the association... more
    Although the cariostatic effects of CO2 laser on the root surface have been shown, there is scarce information regarding its effects on root secondary caries. The objective of this research was to investigate the effect of the association of CO2 laser and a fluoride dentifrice on the inhibition of secondary caries on root surfaces adjacent to composite-resin or glass-ionomer-cement restorations. Dental blocks of human roots were divided into two groups: composite resin (CR) or glass ionomer cement (GIC). Subsequently, the blocks were divided into four subgroups (n = 10): C, non-fluoride dentifrice; FD, fluoride dentifrice; L, CO2 laser with an energy density of 6.0 J cm−2 + non-fluoride dentifrice; and L + FD, CO2 laser + fluoride dentifrice. The blocks were subjected to pH cycling to simulate a high cariogenic challenge. Dental demineralization around the restorations was quantified by microhardness analysis. The results were subjected to analysis of variance (ANOVA) and the Tukey–Kramer test (p ≤ 0.05). As for mineral loss, it can be observed that all the groups that were treated with a fluoride dentifrice and laser, used alone or not, were statistically similar and superior to the RC–C group. It was concluded that CO2 laser irradiation and a fluoride dentifrice used alone or combined with each other are efficient surface treatments for preventing secondary root caries, regardless of the restorative material used.
    Although several conventional surface treatments have been used on feldspathic ceramic, a few studies investigated the effects of the irradiation with Er: YAG laser using different parameters. The aim of this in vitro study was to... more
    Although several conventional surface treatments have been used on feldspathic ceramic, a few studies investigated the effects of the irradiation with Er: YAG laser using different parameters. The aim of this in vitro study was to evaluate the shear bond strength of a resin cement to feldspathic ceramic, after the application of different surface treatments, especially the irradiation with Er:YAG laser. Forty-two discs made of a feldspathic ceramic were divided into six groups (n = 7): G1: control group - 10% hydrofluoric acid (HF), G2: Air abrasion with Al2O3 + HF; G3: Er: YAG laser with 500 mJ/4Hz, G4: Er: YAG laser with 500 mJ/4Hz: + HF; G5: Er: YAG laser with 400 mJ/6Hz and G6: Er:YAG laser with 400 mJ/6Hz + HF. After this, all the specimens were treated with silane, and then a resin cement cylinder was built on the treated ceramic surface. After 24 h at 37 oC, specimens were submitted to the shear bond strenght test and stereoscopic evaluation to determine the type of failure. ...
    This study investigated the effect of CO2laser irradiation on the inhibition of secondary caries on root surfaces adjacent to glass ionomer cement (GIC) or composite resin (CR) restorations. 40 dental blocks were divided into 4 groups: G1... more
    This study investigated the effect of CO2laser irradiation on the inhibition of secondary caries on root surfaces adjacent to glass ionomer cement (GIC) or composite resin (CR) restorations. 40 dental blocks were divided into 4 groups: G1 (negative control): cavity preparation + adhesive restoration with CR; G2: (positive control) cavity preparation + GIC restoration; G3: equal to group 1 + CO2laser with 6 J/cm2; G4: equal to group 2 + CO2laser. The blocks were submitted to thermal and pH cycling. Dental demineralization around restorations was quantified using microhardness analyses and Light-Induced Fluorescence (QLF). The groups showed no significant differences in mineral loss at depths between 20 μm and 40 μm. At 60 μm, G2 and G3 ≠ G1, but G4 = G1, G2 and G3. At 80 μm, G4 ≠ G1, and at 100 μm, G4 = G2 = G1. At 140 and 220 μm, G2, G3, and G4 = G1. The averages obtained using QFL in groups 1, 2, 3, and 4 were 0.637, 0.162, 0.095, and 0.048, respectively. QLF and microhardness anal...
    Estudos têm mostrado que a dentina pode ser modificada pelo laser pulsado de CO2 tornando-a um substrato mais ácido-resistente. Este estudo in vitro se propôs a avaliar o efeito do laser de CO2, associado ou não à aplicação tópica de... more
    Estudos têm mostrado que a dentina pode ser modificada pelo laser pulsado de CO2 tornando-a um substrato mais ácido-resistente. Este estudo in vitro se propôs a avaliar o efeito do laser de CO2, associado ou não à aplicação tópica de flúor, na redução da desmineralização de superfícies radiculares humanas, utilizando um laser pulsado de comprimento de onda 10,6 m. Cinquenta superfícies radiculares humanas obtidas de vinte e cinco terceiros molares foram aleatoriamente divididas em 5 grupos (n=10) com os seguintes tratamentos de superfície: G1 Nenhum tratamento (controle negativo), G2 Flúor Fosfato Acidulado (FFA- controle positivo), G3 Laser 4,0 J/cm2, G4 Laser 4,0 J/cm2 + FFA e G5 FFA + Laser 4,0 J/cm2. Após o tratamento da superfície, os espécimes foram submetidos a 7 dias de ciclagem de pH, permanecendo diariamente em soluções desmineralizadora e remineralizadora por 3 h e 21 h, respectivamente. Após o desafio ácido, os espécimes foram avaliados para determinar a perda mineral po...
    Viruses can spread to the environment, and it can be challenging to clear it. A direct approach to limit airborne transmission of pathogens in dental clinic offices is to inactivate viruses within a short time of their production and... more
    Viruses can spread to the environment, and it can be challenging to clear it. A direct approach to limit airborne transmission of pathogens in dental clinic offices is to inactivate viruses within a short time of their production and block the person-to-person transmission routes in dental clinics. For this, we can use chemical substances on surfaces and germicidal ultraviolet light (UV), typically at 254 nm, for complementary disinfection of surfaces and air contaminated by aerosols produced by high-speed handpiece or ultrasound scaler. Based on the literature review and the similarity of Sars-Cov-2 with other previously studied corovaviruses, COVID-19 is sensitive to UV irradiation that can break the genome of this virus, inactivating it. In our study, we performed the calculation of the time required to decontaminate a dental care room between each patient change. We can conclude that the use of UVC can be incorporated into the dental care routine to reduce cross contamination.Ke...