Natural products can be used as complements or as alternatives to synthetic drugs. Eugenia uniflora and Tropaeolum majus are natives of Brazil and have antimicrobial, anti-inflammatory, and antioxidant activities. This study aimed to... more
Natural products can be used as complements or as alternatives to synthetic drugs. Eugenia uniflora and Tropaeolum majus are natives of Brazil and have antimicrobial, anti-inflammatory, and antioxidant activities. This study aimed to develop a film-forming system (FFS) loaded with plant extracts with the potential for treating microbial infections. E. uniflora and T. majus leaf extracts were prepared and characterized, and the individual and combined antioxidant and antimicrobial activities were evaluated. The FFS was developed with different concentrations of polyvinylpyrrolidone (PVP) and polyvinyl alcohol (PVA) and analyzed for physicochemical characteristics. The combination of extracts showed a superior antioxidant effect compared to the individual extracts, justifying the use of the blend. FFS prepared with 4.5% PVA, 4.5% PVP, 7.81% E. uniflora extract, and 3.90% T. majus extract was adhesive, lacked scale formation, presented good malleability, and had a suitable pH for topic...
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O tratamento de doenças inflamatórias se dá por meio de fármacos anti-inflamatórios, que exercem inúmeros efeitos colaterais que podem restringir seu uso e a duração. Diante disso, com o intuito de melhorar a saúde dos pacientes que... more
O tratamento de doenças inflamatórias se dá por meio de fármacos anti-inflamatórios, que exercem inúmeros efeitos colaterais que podem restringir seu uso e a duração. Diante disso, com o intuito de melhorar a saúde dos pacientes que sofrem de distúrbios inflamatórios como a artrite reumatoide (AR) e a osteoartrite (OA), o presente trabalho teve como objetivo realizar um levantamento na literatura científica disponível, das principais espécies medicinais com ação anti-inflamatória contra doenças articulares, cultivadas no Horto Medicinal do Campus 2 da UNIPAR – Universidade Paranaense/Umuarama. Dentre as 92 espécies do Horto, empregadas como anti-inflamatória, 6 foram selecionadas: Zingiber officinale, Curcuma longa, Baccharis trimera, Solidago chilenses, Cordia verbenacea, e Echinodorus grandiflorus. Os estudos demonstraram que as plantas medicinais supracitadas possuem efeitos na atenuação dos sintomas e patogênese de pacientes que possuem AR e OA, cujo compostos bioativos presente...
A beleza está diretamente ligada ao aspecto dos cabelos, em todos os tempos e todas as culturas. O cabelo ao longo da vida é sujeito a vários tipos de mudanças, sejam elas naturais ou artificiais, conduzidas por uma vontade de mudar,... more
A beleza está diretamente ligada ao aspecto dos cabelos, em todos os tempos e todas as culturas. O cabelo ao longo da vida é sujeito a vários tipos de mudanças, sejam elas naturais ou artificiais, conduzidas por uma vontade de mudar, muitas vezes, seja por representar uma nova fase, como também é símbolo de autocuidado, vaidade, sucesso, possuem significativa importância na aparência e autoestima das pessoas e, por isso, a queda capilar pode atingir a qualidade de vida do indivíduo. Neste sentido, o objetivo deste estudo foi avaliar procedimentos estéticos minimamente invasivos e não cirúrgicos com ênfase na queda capilar. Para tanto, foi realizada uma revisão bibliográfica integrativa, de caráter descritivo nas principais bases de dados em saúde: SciELO, Google Acadêmico, PubMed e livros de medicina, nos idiomas português, inglês e espanhol, considerando o período de 2007 a 2022. De acordo com o último censo da Sociedade Brasileira de Dermatologia, a queixa de alopecia está entre a...
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Através da edição da Norma Operacional de Assistência à Saúde realizada em 2001 e da publicação do Pacto pela Saúde realizado em 2006 o processo de acesso à saúde, ganhou maior ênfase no quesito de inovações e melhorias do sistema de... more
Através da edição da Norma Operacional de Assistência à Saúde realizada em 2001 e da publicação do Pacto pela Saúde realizado em 2006 o processo de acesso à saúde, ganhou maior ênfase no quesito de inovações e melhorias do sistema de saúde. A assistência farmacêutica sobreveio como parte fundamental nos serviços e programas de saúde. Neste sentido, o objetivo do presente estudo foi de expor as principais políticas públicas acerca da temática de medicamentos essenciais. Para tanto, foi realizada uma revisão integrativa de literatura, tendo como base de dados o Ministério da Saúde, as Resoluções da Agência Nacional de Vigilância Sanitária, artigos científicos e as publicações da Relação Nacional de Medicamentos (RENAME). No ano de 1998 através da portaria nº 3.916, de 30 de outubro 1998, apresenta-se a população a terminologia de Políticas públicas e, como resposta às diretrizes resultantes desta política, no presente artigo teremos ênfase na RENAME, criada através da portaria nº 3....
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A pílula contraceptiva de emergência (CE) é chamada pelos médicos de pílula anticoncepcional pós-coito. É um método anticoncepcional de emergência e deve ser tomada até 72 horas após a relação sexual. É adequado para casos de abuso... more
A pílula contraceptiva de emergência (CE) é chamada pelos médicos de pílula anticoncepcional pós-coito. É um método anticoncepcional de emergência e deve ser tomada até 72 horas após a relação sexual. É adequado para casos de abuso sexual, preservativos rompidos ou falha de outros métodos anticoncepcionais. Sendo assim, o objetivo do presente estudo foi avaliar o perfil da dispensação do contraceptivo de emergência (CE) em uma farmácia comercial localizada em um distrito do município de Assis Chateaubriand no oeste do estado do Paraná. Para o desenvolvimento da pesquisa, foi avaliado a quantidade do medicamento dispensado com o princípio ativo levonorgestrel no período de três meses em uma farmácia de dispensação de medicamentos no distrito de Bragantina, assim como a faixa etária da paciente que utilizou, o seu estado civil e o motivo da compra da pílula. O projeto foi aprovado pelo Comitê de ética sob o número do CAAE: 60509322.3.0000.0109. Os resultados mostraram que no período d...
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In December 2019, a new coronavirus originating from the city of Wuhan in China started an epidemic that brought many countries into chaos and despair. SARS-CoV-2, as identified, gave rise to the severe acute respiratory syndrome called... more
In December 2019, a new coronavirus originating from the city of Wuhan in China started an epidemic that brought many countries into chaos and despair. SARS-CoV-2, as identified, gave rise to the severe acute respiratory syndrome called COVID-19. Its transmission happens through droplets of saliva, hand or contaminated surfaces. Since its discovery, COVID-19 has led many to death, therefore, researchers from around the world have joined efforts to develop strategies to contain the virus. In this race, drugs such as Chloroquine and Hydroxychloroquine have become possible options for showing an antiviral effect, however, studies contest their efficiency, generating uncertainties. Therefore, other alternatives have been investigated in this context, and the study of medicinal plants has been the target of research for the treatment of COVID-19 in search of bioactive natural products that can exert an antiviral action. The study aimed to analyze the published literature on COVID-19 (SAR...
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Pelas características anatômicas e fisiológicas dos rins, a lesão renal aguda tem sua origem nefrotóxica pela alta circulação local, o que favorece a alta concentração de substâncias tóxicas e seus metabólitos no tecido. A lesão renal... more
Pelas características anatômicas e fisiológicas dos rins, a lesão renal aguda tem sua origem nefrotóxica pela alta circulação local, o que favorece a alta concentração de substâncias tóxicas e seus metabólitos no tecido. A lesão renal aguda é uma complicação comum em internações hospitalares e principalmente em internações em unidades de terapia intensiva. A ciclofosfamida, um quimioterápico utilizado no tratamento de doenças autoimunes e neoplasias sólidas, pode causar nefrotoxicidade com disfunção glomerular e tubular. O uso de plantas medicinais, pelas suas potentes ações antioxidantes, tem sido usado para prevenção ou tratamento de lesões celulares induzidas pelo desequilíbrio entre enzimas antioxidantes e oxidantes. Por esse motivo, o objetivo do experimento foi avaliar o potencial efeito protetor da Echinodorus grandiflorus na prevenção da nefrotoxidade induzida pela ciclofosfamida. Para isso, foi realizado o experimento com a utilização de 35 ratos machos, Wistar, divididos e...
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A cistite hemorrágica e a cistite intersticial expressam uma etiologia variável, desde idiopática à provocada por fármacos, dentre eles a ciclofosfamida. A cistite apresenta tratamento multifatorial, e o potencial efeito satisfatório do... more
A cistite hemorrágica e a cistite intersticial expressam uma etiologia variável, desde idiopática à provocada por fármacos, dentre eles a ciclofosfamida. A cistite apresenta tratamento multifatorial, e o potencial efeito satisfatório do uso da medicina complementar, vem ganhando espaço na prática médica. Assim o objetivo do presente estudo foi avaliar o efeito protetivo do extrato bruto de Echinodorus grandiflorus sobre a bexiga de ratos induzidos a cistite por ciclofosfamida. Utilizou-se neste estudo, 35 ratos, machos, Wistar, com peso médio de 321g, que foram submetidos a indução de cistite com uso de ciclofosfamida por via intraperitoneal e tratados com diferentes doses de extrato de Echinodorus grandiflorus (30, 100, 300mg) e o grupo controle com o fármaco Mesna.Todos os animais foram mortos no décimo sétimo dia e suas bexigas urinarias foram ressecadas para avaliação macro e microscópica, além da análise de hemograma e leucograma. A análise do sangue mostrou leucopenia com dife...
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Esse estudo objetivou explorar os impasses e desafios sobre o processo de inclusão escolar de alunos com transtorno do espectro do autista (TEA) na cidade de Umuarama-PR durante a pandemia da COVID-19. Os voluntários foram os pais desses... more
Esse estudo objetivou explorar os impasses e desafios sobre o processo de inclusão escolar de alunos com transtorno do espectro do autista (TEA) na cidade de Umuarama-PR durante a pandemia da COVID-19. Os voluntários foram os pais desses alunos e os responsáveis das 20 escolas municipais de Umuarama por meio de um questionário para compreender o funcionamento da inclusão escolar desses alunos e quais aspectos poderiam ser melhorados. O questionário baseava-se em perguntas objetivas e descritivas que buscavam compreender melhor quais os desafios passados tanto pelos pais como pelos diretores das escolas, dessa forma foram obtidas 16 respostas de pais dos alunos, sendo que temos mais de 100 alunos com TEA cadastrados na rede municipal, evidenciando-se a baixa adesão por parte da família e ficando nítido como ainda é difícil a aceitação dos mesmos sobre o assunto. Em relação às escolas municipais apenas 9 das 18 responderam os questionamentos, sendo que a maioria das respostas eram bem...
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Arterial hypertension (AH) is a polygenic syndrome and comprises several pathophysiological aspects such as genetic predisposition, where heredity plays a considerable role in the origin of the increase in blood pressure levels. AH is the... more
Arterial hypertension (AH) is a polygenic syndrome and comprises several pathophysiological aspects such as genetic predisposition, where heredity plays a considerable role in the origin of the increase in blood pressure levels. AH is the most frequent disease in Brazilian society, and its exact prevalence is not known throughout the country, and it contributes significantly to a high rate of cardiovascular mortality throughout the country. The evaluation of a patient with AH includes confirmation of the diagnosis, suspicion and identification of a secondary cause, in addition to checking the risk of a cardiovascular attack. Non-adherence to treatment can also be linked to the undesirable effects of antihypertensive drugs, financial barriers, lack of motivation of the patient to treat an asymptomatic disease, long-term treatment, influence on quality of life, inadequate relationship with the health team, among other diverse factors. Thus, the present study aims to search the scienti...
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Introdução: O aumento da resistência bacteriana aos antibióticos tem se tornado uma ameaça à população. Devido ao grande avanço da fitoterapia nos últimos anos, estudos sobre a ação antimicrobiana de diferentes bioativos tem sido... more
Introdução: O aumento da resistência bacteriana aos antibióticos tem se tornado uma ameaça à população. Devido ao grande avanço da fitoterapia nos últimos anos, estudos sobre a ação antimicrobiana de diferentes bioativos tem sido incentivado cada vez mais. De acordo com a literatura, a E. uniflora apresenta composição química complexa tanto nos frutos quanto nas folhas. Seus compostos estão relacionados a atividades terapêuticas já comprovadas cientificamente, destacando-se as ações anti-inflamatória, hipotensora, hipoglicemiante, antipirética e antibacteriana. Com relação ao própolis já foram encontrados e identificados cerca de 300 compostos relacionados a diversos benefícios e atividades farmacológicas, como anti-inflamatória, antioxidante, antifúngica, hepatoprotetora, antisséptica, antiviral, antibacteriana, entre outras. Objetivo: Avaliar a atividade antimicrobiana individual e o efeito sinérgico dos extratos de Eugenia uniflora e Própolis por metodologia de checkerboard. Meto...
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Background: Due to the bacterial resistance to conventional antibiotics, studies on natural products with antibacterial or bactericidal activity are becoming more and more frequent. Among multi-resistant bacteria, Escherichia coli is a... more
Background: Due to the bacterial resistance to conventional antibiotics, studies on natural products with antibacterial or bactericidal activity are becoming more and more frequent. Among multi-resistant bacteria, Escherichia coli is a producer of extended-spectrum beta-lactamase (ESBL). Papain, coming from papaya latex (Carica papaya), stands out for its capacity to degrade the devitalized tissue that delays the healing process. Parsley (Petroselinum crispum) has been studied, mainly for its diuretic, antioxidant and antimicrobial properties. The aim of this work was to report the use of these two phytotherapic agents in an equine presenting abscess infected by multi-resistant ESBL producing E. coli.Case: A four and a half year old male neutered equine weighing 400 kg of undefined race (SRD) was admitted to the Veterinary Hospital of the Paranaense University (UNIPAR), presenting an increased volume on the left side of the middle third of the neck, one year ago, probably due to an ...
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Objetivo: avaliar a atividade acaricida e larvicida do óleo essencial das folhas de Psidium rufum frente a fêmeas ingurgitadas e larvas de Rhipicephalus microplus. Metodologia: o óleo essencial das folhas frescas de P. rufum foi extraído... more
Objetivo: avaliar a atividade acaricida e larvicida do óleo essencial das folhas de Psidium rufum frente a fêmeas ingurgitadas e larvas de Rhipicephalus microplus. Metodologia: o óleo essencial das folhas frescas de P. rufum foi extraído por hidrodestilação (2h) e analisado por cromatografia em fase gasosa acoplada à espectrometria de massas (CG-EM). As atividades acaricida e larvicida foram realizadas pela técnica de imersão de adultos e imersão larval (Larval Paket Test) utilizando o óleo essencial nas concentrações de 300 a 0,001 mg/mL. Como controle positivo foi utilizado uma solução comercial (cipermetrina 15%; clorpirifós 25%; citronelal 1%) a 0,005%, e como controle negativo uma solução aquosa de polissorbato (80) a 2,0%. As concentrações letais (CL50 e CL99) foram determinadas pela análise de Probitos. Resultados: no óleo essencial, foram identificados 55 compostos, sendo a classe majoritária os sesquiterpenos hidrocarbonetos (70,07%), e como majoritários o 1,8-cineole (19,0...
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With the advancement of medicine, the vaccine against human papillomavirus (HPV) was developed. HPV is a sexually transmitted virus and the vaccine is a skillful means of preventing transmission. However, the vaccine needs to be applied... more
With the advancement of medicine, the vaccine against human papillomavirus (HPV) was developed. HPV is a sexually transmitted virus and the vaccine is a skillful means of preventing transmission. However, the vaccine needs to be applied to the at-risk population, young women and men. Therefore, the objective of the present study is to highlight comprehensive studies on the applicability of the vaccine to the population in an efficient manner. The research will approach a wide review of scientific articles published in recent years, the collection will be carried out in the Google Scholar Database, MEDLINE and SciELO, allowing a discourse analysis of the selected works, and thus, building a critical analysis resulting from the information gathered about the population at risk, effectiveness and stigmas of non-vaccination. In a critical analysis, it was corroborated that although vaccination has proven to be effective, the population at risk is not totally immune to the virus, since t...
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Currently the accelerated and troubled routine ends up also affecting children and adolescents, who are still unable to develop, express and assimilate what happens to them, internally and externally, understand their pains and suffering;... more
Currently the accelerated and troubled routine ends up also affecting children and adolescents, who are still unable to develop, express and assimilate what happens to them, internally and externally, understand their pains and suffering; thus, they end up developing mental disorders, such as depression. Given the importance of seeking more information about depression, this study aimed to produce a bibliographic review article on depression in childhood and adolescence and its characteristics. The databases Pubmed (US National Library of Medicine), Scielo (Scientific Electronic Library Online), and Google ScholarⓇ were used in the present study, using the descriptors: depression, psychotropic drugs, mental disorders and psychotherapy. The articles analyzed were written in Portuguese, English and Spanish, for the past 22 years. For the diagnosis of children or adolescents, the clinical investigation has its particularities, the anamnesis must be more in-depth and it must go beyond t...
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Cecropia pachystachya Trécul is popularly known in Brazil as embaúva or embaúba. Pharmacological activities were reported in extant studies including the development of anxiolytic, antidepressant, hypoglycemic, antioxidant, cardiotonic,... more
Cecropia pachystachya Trécul is popularly known in Brazil as embaúva or embaúba. Pharmacological activities were reported in extant studies including the development of anxiolytic, antidepressant, hypoglycemic, antioxidant, cardiotonic, sedative, anti-inflammatory, antimicrobial and leishmanicidal properties for aqueous and alcoholic extracts prepared by infusion or maceration of C. pachystachya leaves. However, there is a paucity of studies examining extracts of leaves of C. pachystachya obtained by supercritical fluid technology. This study evaluated the extraction of Cecropia pachystachya oil using supercritical carbon dioxide (CO2). Extractions were performed at 35 °C, 45 °C, and 55 °C and 14 MPa, 17 MPa, and 20 MPa. The extraction yield, characteristics of the oil, antioxidant and antibacterial potentials were evaluated. The highest extraction yield obtained from C. pachystachya leaves was 1.48% using supercritical CO2 at 55 °C and 20 MPa. The main identified compounds include ...
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Therapeutic approaches for the treatment of dyslipidemia and atherosclerosis have radically changed in recent decades. Part of this advance undeniably stems from basic biomedical research that has provided a better understanding and... more
Therapeutic approaches for the treatment of dyslipidemia and atherosclerosis have radically changed in recent decades. Part of this advance undeniably stems from basic biomedical research that has provided a better understanding and identification of new therapeutic targets. The aim of this work was to develop a model to induce atherogenesis and hepato-renal impairment in female Wistar rats. The following groups received the respective treatments for 60 days: control animals, non-ovariectomized rats that received an atherogenic diet (NEAD), ovariectomized rats that received an atherogenic diet (NOAD), non-ovariectomized rats that received an atherogenic diet and oral Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME; LEAD), and ovariectomized rats that received an atherogenic diet and oral l-NAME (LOAD). Animals in the NEAD, NOAD, LEAD, and LOAD groups also received methimazole and cholecalciferol daily. Urinary, biochemical, hemodynamic, and electrocardiographic parameters and...
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This study aimed to macroscopically and microscopically evaluate the healing of skin wounds induced in rats by topical application of cassava polyamide biopolymer hydrogel. In total, 32 rats were used and divided into four groups (n= 8):... more
This study aimed to macroscopically and microscopically evaluate the healing of skin wounds induced in rats by topical application of cassava polyamide biopolymer hydrogel. In total, 32 rats were used and divided into four groups (n= 8): negative control - saline solution; positive control - use of commercial ointment; experimental group - I - ointment + cassava hydrogel; experimental group - II - cassava hydrogel. A 1cm2 wound induced on the animals dorsum was treated and evaluated. At day 21 post-operation, the animals were sacrificed by anesthetic overdose, and then 1cm2 of cicatricial skin from the wound region was collected. The material was cut to evaluate healing. In the macroscopic evaluation, complete healing was observed at the end of 21 days. Re-epithelialization was observed histologically; the connective tissue in the negative control, positive, and experimental - I groups was characterized by an abundance of collagen fibers, fibroblasts, and blood vessels. In experimen...
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Lamiaceae and Euphorbiaceae are two families of plants grown as medicinal, aromatic, food and ornamental crops, and are of great commercial importance. The cultivation of these species depends on soil quality and availability of soil... more
Lamiaceae and Euphorbiaceae are two families of plants grown as medicinal, aromatic, food and ornamental crops, and are of great commercial importance. The cultivation of these species depends on soil quality and availability of soil resources. Arbuscular mycorrhizal fungi (AMF) usually penetrates plants through their roots, supplying plants with water and nutrients and receiving photosynthesis products in return. These types of symbiosis benefit the development and production of crops. To analyze the effects of AMF inoculation on the production of plants of these families, a meta - analysis was performed using 183 data of Lamiaceae plants and 68 data of Euphorbiaceae plants. Meta-analysis consists of compiling data from literature to obtain the response ratio, calculated by the mean of the experimental group divided by the mean of the control group. The response variables were shoot dry mass (SDM), total dry mass (TDM), plant height and accumulation of phosphorus in the plant shoot...
Research Interests: Botany, Plant Biology, Biology, Medicinal Plants, Lamiaceae, and 3 moreEuphorbiaceae, April, and Shoot
It is well established that the behavior of different mammalians, including rodents, may undergo profound changes in the presence of individuals of other species. Therefore, the aim of this study was to verify if the presence of... more
It is well established that the behavior of different mammalians, including rodents, may undergo profound changes in the presence of individuals of other species. Therefore, the aim of this study was to verify if the presence of pheromones from male mice could influence the reproductive parameters of female rats. Twenty Swiss (SW) male mice and 16 Long Evans (LE) female Rats were housed in separate rooms, with communication in the ceiling in its entire length. A continuous flow of air was planned to pass through the room of the mice before the room of the rats and finally be exchanged with the external environment. During 45 days, vaginal smears were collected. Relative weights of reproductive organs, estradiol, progesterone, and dehydroepiandrosterone levels were also measured. The total duration of the estrous cycle and relative organ weight remained unchanged. On the other hand, the proestrus and estrus phases, as well as the estradiol levels were increased, while the diestrus ph...
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Medicinal plants have great prominence in research into the development of new medicines. Eugenia uniflora L. (Myrtaceae) is an edible and medicinal plant with economic value in the northeast region of Brazil. Several preparations from E.... more
Medicinal plants have great prominence in research into the development of new medicines. Eugenia uniflora L. (Myrtaceae) is an edible and medicinal plant with economic value in the northeast region of Brazil. Several preparations from E. uniflora leaves and its fruits are employed as a source of nutrients and bioactive compounds. In this study we evaluated the preclinical toxicology of crude extract and vaginal gel obtained from the leaves of E. uniflora (5%, 10%, and 15%) aiming to provide safety for its use in the treatment of vulvovaginitis. Both formulations were applied to the vaginal cavity for 14 days. Detailed observations of the vaginal region, including pruritus, swelling, irritation, burning, pain, and vaginal secretion, as well as the estrous cycle were evaluated. On the fifth day, blood samples were obtained from the supraorbital plexus for biochemical and hematological analyses. The animals were subsequently euthanized. All animals underwent necropsy and macroscopic e...
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Research Interests: Chemistry, Analytical Chemistry, Chromatography, Mass Spectrometry, Sample Preparation, and 15 moreQuantitative analysis, In Vitro, Gas Chromatography, Chemical Analysis, Coefficient of Variation, Phenol, Phenolic compound, Derivatization, Biological Monitoring, Quantitative Analysis, Acetic Acid, Catechol, Hydroquinone, Acid hydrolysis, and limit of detection
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Heart failure is a chronic disease that compromises the patient's quality of life, due to its symptoms, such as: tiredness/fatigue, exercise intolerance, orthopnea, paroxysmal nocturnal dyspnea. The aim of the present study was to... more
Heart failure is a chronic disease that compromises the patient's quality of life, due to its symptoms, such as: tiredness/fatigue, exercise intolerance, orthopnea, paroxysmal nocturnal dyspnea. The aim of the present study was to intervene and assist in the treatment of Heart Failure patient with pharmacological and non-pharmacological approaches in order to improve the quality of life and patient compliance regarding the existing disease. Pharmaceutical consultations were carried out over a seven-month period with a patient that suffers from Heart Failure, resident of Umuarama-PR, treated at home. The SOAP method was adopted for the registration of consultations for later analysis, and the measurement of blood pressure was performed by the patient, using the HBPM method. For the analysis of interactions, the Micromedex database and the patient’s package leaflet were used.The patient had a significant decrease in his BP with the follow-up; however, he showed resistance to going...
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This study aims to carry out a bibliographic survey on ethnobotanical, ethnopharmacological and pharmacological information on Rosaceae species. The species addressed were Eriobotrya japonica (yellow-plum), Fragaria vesca (strawberry),... more
This study aims to carry out a bibliographic survey on ethnobotanical, ethnopharmacological and pharmacological information on Rosaceae species. The species addressed were Eriobotrya japonica (yellow-plum), Fragaria vesca (strawberry), Malus domestica (apple), Prunus domestica (plum), Prunus persica (peach), Pyrus communis (pear) and Rubus brasiliensis (raspberry) grown in the garden Medicinal of Universidade Paranaense (UNIPAR) - Campus 2. For this study, the databases were taken from national and international scientific journals without restriction of year of publication. As a result, a category of use was identified, part used, form of preparation, popular use, pharmacological and phytochemical studies for each species. Thus, it is observed that all fruit species are popularly used a medicinal, with records of ethnopharmacological, pharmacological and phytochemical studies. Medicinal plants are very widespread and used, being considered as an important therapeutic resource. Howe...
Research Interests: Ethnobotany, Biology, Society Development, Phytochemical, Rosaceae, and 4 morePrunus, Rubus, Pear, and Fruit Tree
BACKGROUND Nothofagin is a mono-C-glycoside of 4,2',4',6'-tetrahydroxy- dihydrochalcone that is commonly found in Aspalathus linearis, Nothofagus fusca, and Leandra dasytricha. A wide range of biological effects has been... more
BACKGROUND Nothofagin is a mono-C-glycoside of 4,2',4',6'-tetrahydroxy- dihydrochalcone that is commonly found in Aspalathus linearis, Nothofagus fusca, and Leandra dasytricha. A wide range of biological effects has been attributed to nothofagin, including antioxidant, diuretic, renoprotective, antiplatelet, and antithrombotic effects. Although nothofagin is pharmacologically active, its effects on blood pressure remain unknown. In the present study, we investigated whether nothofagin causes acute and prolonged hypotension in male Wistar rats, and we investigated the molecular mechanisms that underlie these hemodynamic effects. METHODS Hypotensive effects of nothofagin (0.3, 1, and 3 mg/kg) were evaluated after acute intraduodenal administration and after 7 days of oral treatment. Using pharmacological antagonists and inhibitors, we explored the involvement of the prostaglandin/cyclic adenosine monophosphate and nitric oxide/cyclic guanosine monophosphate pathways and K+ channels in nothofagin-induced hypotension. RESULTS Acute and prolonged nothofagin administration significantly decreased systolic blood pressure and mean arterial pressure in Wistar rats. Pretreatment with N(G)-nitro-L- arginine methyl ester, methylene blue, and tetraethylammonium prevented the hypotensive effect of nothofagin. CONCLUSIONS These results shows that nothofagin induces a hypotensive response in Wistar rats, and this effect depends on K+ channel opening in smooth muscle cells through nitric oxide signaling.
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According to the World Health Organization, researchers estimate that the spread of vaccines has allowed worldwide life expectancy to increase by 30 years in the past two centuries. Despite these advances, there are major challenges to be... more
According to the World Health Organization, researchers estimate that the spread of vaccines has allowed worldwide life expectancy to increase by 30 years in the past two centuries. Despite these advances, there are major challenges to be overcome to expand immunization coverage. Currently, the Unified Health System (SUS) offers a total of 19 vaccines that protect against more than 40 diseases free of charge. Therefore, the objective of the study was to study and analyze the current state of Brazilian vaccination in relation to infectious diseases eradicated in the country. For the development of the work, data from the Ministry of Health, published scientific articles in the Google academic, SCIELO and PubMed databases, epidemiological bulletins, as well as relevant studies and reports published on websites and internet portals through the descriptors, vaccination in Brazil, vaccination delay in Brazil, vaccines available in the single system were used (SUS). Through this work, it ...
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Echinodorus grandiflorus has pharmacological properties due to its secondary metabolism, such as anti-inflammatory, antioxidant, diuretic, analgesic, anti-rheumatic, antihypertensive, and cardioprotective effects. The aim of this study... more
Echinodorus grandiflorus has pharmacological properties due to its secondary metabolism, such as anti-inflammatory, antioxidant, diuretic, analgesic, anti-rheumatic, antihypertensive, and cardioprotective effects. The aim of this study was to determine the phytochemical profile and evaluate the antimicrobial activity of crude extract of E. grandiflorus form its leaves. In the analysis of the phytochemical profile, qualitative tests were performed to identify tannins, alkaloids, flavonoids, anthraquinones, steroids, triterpenes, saponins, polysaccharides, and coumarins. Antimicrobial tests were performed using the disk diffusion method and minimum inhibitory concentration (MIC) in 96-well microplates, using hydroalcoholic crude extract obtained by maceration in the proportions 1:5 and 1:10. The higher content of crude extract was observed by maceration 1:5 (3.26%). In phytochemical tests, the presence of tannins, alkaloids, flavonoids, and saponins was detected. The microbial strains...
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Echinodorus grandiflorus is an important medicinal plant species that is native to South America. Despite extensive popular usage as a hypolipidemic drug, its effects as an atheroprotective agent remain unknown. The aim of this study was... more
Echinodorus grandiflorus is an important medicinal plant species that is native to South America. Despite extensive popular usage as a hypolipidemic drug, its effects as an atheroprotective agent remain unknown. The aim of this study was to evaluate the effects of an ethanol-soluble fraction that was obtained from E. grandiflorus (ESEG) leaves against the development of atherosclerosis in rabbits. Male rabbits received a diet that was supplemented with 1% cholesterol (cholesterol-rich diet [CRD]) for 60 days. After 30 days of the CRD, the animals were divided into five groups (n = 6) and treated with ESEG (10, 30, and 100 mg/kg), simvastatin (2.5 mg/kg), or vehicle once daily for 30 days. The negative control group was fed a cholesterol-free diet and treated orally with vehicle. At the end of 60 days, serum lipids, oxidized low-density lipoprotein, thiobarbituric acid reactive substances, nitrotyrosine, and serum interleukin 1 beta (IL-1β), IL-6, soluble intercellular adhesion molecule-1 (sICAM-1), and soluble vascular cell adhesion molecule-1 (sVCAM-1) levels were determined. Samples from the aortic arch and thoracic segment were also collected to investigate the tissue antioxidant defense system and perform histopathological analysis. Oral ESEG administration significantly reduced serum lipid levels in CRD-fed rabbits. This treatment also modulated the arterial antioxidant defense system by reducing lipid and protein oxidation. Similarly, serum IL-1β, IL-6, sICAM-1, and sVCAM-1 levels significantly decreased, accompanied by a reduction of atherosclerotic lesions in all arterial branches. These findings suggest that ESEG may be a new herbal medicine that can be directly applied for the treatment and prevention of atherosclerotic disease.
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Excess weight and dyslipidemia are among the most serious health problems in Western societies. These conditions enhance the risk of cardiac disease and have been linked with a higher prevalence of cardiac arrhythmias and sudden death.... more
Excess weight and dyslipidemia are among the most serious health problems in Western societies. These conditions enhance the risk of cardiac disease and have been linked with a higher prevalence of cardiac arrhythmias and sudden death. The present study investigated the cardioprotective effects of on ventricular remodeling in rabbits that were fed a 1% cholesterol-rich diet. We first obtained an ethanol-soluble fraction of and performed a detailed phytochemical study by liquid chromatography-DAD/ESI-MS. For 60 days, male rabbits were fed the cholesterol-rich diet or a diet without the addition of cholesterol. After 30 days, different groups of rabbits were treated with the ethanol-soluble fraction of (10, 30, and 100 mg/kg, p. o.), simvastatin (2.5 mg/kg), or vehicle once daily for 30 days. At the end of 60 days, the serum lipoprotein ratio, electrocardiographic profile, histopathological alterations, and the cardiac antioxidant defense system were investigated. Echocardiographic an...
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Tropaeolum majus L., popularly known as nasturtium, is a species widely used in the form of infusions and salads. In the last years, the antihypertensive, diuretic, and calcium and potassium sparing activities of T. majus preparations... more
Tropaeolum majus L., popularly known as nasturtium, is a species widely used in the form of infusions and salads. In the last years, the antihypertensive, diuretic, and calcium and potassium sparing activities of T. majus preparations were shown. Moreover, no preclinical 90-day oral toxicity studies were conducted. Thus, this study evaluated the toxicity of the hydroethanolic extract obtained from T. majus (HETM) leaves in female and male mice, rats, and rabbits. Swiss mice and Wistar rats were treated with HETM (75, 375, and 750 mg/kg). The doses of rabbits (30, 150, and 300 mg/kg) were calculated by allometric extrapolation. The control groups received vehicle. The animals were orally treated, daily, for 90 days. At the end, the animals were anesthetized, and body weight gain, relative weight of liver, kidney, and spleen, and histopathological changes were evaluated. Serum hematological and biochemical parameters were also analyzed. No alterations were found in body and organ weig...
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A gestação é um período único e especial na vida de cada mulher, no qual ocorre uma série de modificações no organismo feminino, tanto do ponto de visto físico como do ponto de vista psíquico, que começam na primeira semana e continuam... more
A gestação é um período único e especial na vida de cada mulher, no qual ocorre uma série de modificações no organismo feminino, tanto do ponto de visto físico como do ponto de vista psíquico, que começam na primeira semana e continuam durante todo o período de gestação. Essas modificações ocasionam uma série de desconfortos (dores musculares, enjoos, vômitos, e constipação) que interferem no estado físico e emocional da gestante. Na busca por aliviá-las muitas gestantes buscam consumir produtos de origem natural por acreditarem que eles não fazem mal. Nessa busca, a crença de que é “natural” é sinônimo de “seguro” tornam o consumo de plantas medicinais atraente para muitas gestantes, que ao consumirem esses produtos, muitas vezes, sem orientação médica ou farmacêutica, acreditam não existir riscos ao embrião/feto e para si mesmas. Isso faz com que o uso medicinal de plantas seja comum na gestação. Porém, existem evidências científicas que muitas substâncias presentes nas plantas me...
Research Interests: Philosophy and Humanities
Poucos grupos de pesquisa no Brasil se dedicam ao estudo e desenvolvimento de novas drogas diuréticas a partir de produtos naturais, incluindo as plantas medicinais. O motivo dessa carência de investigação frente a imensa biodiversidade... more
Poucos grupos de pesquisa no Brasil se dedicam ao estudo e desenvolvimento de novas drogas diuréticas a partir de produtos naturais, incluindo as plantas medicinais. O motivo dessa carência de investigação frente a imensa biodiversidade brasileira não está totalmente esclarecida, mas a falta de uma padronização metodológica pode ter contribuído de forma substancial para a ausência de um fitoterápico disponível para comercialização devido suas propriedades diuréticas. “Desenvolvimento de Fármacos Diuréticos a Partir de Produtos Naturais” pretende colmatar a falta de uma publicação em língua portuguesa que aborde este tema de uma maneira cientificamente correta e independente. Isto, em uma época em que os recursos destinados aos designados “Herbal medicines”, medicamentos à base de plantas, volta a merecer atenção dos leigos e das autoridades de saúde de todo o mundo.
Research Interests: Pharmacology, Drug interactions, Medicine, Fetal development, Pregnancy, and 15 moreToxicity, Reproductive toxicology, Female, Animals, Embryonic Development, Rats, Antimalarials, Teratogens, Artesunate, Bone and Bones, Developmental toxicity, Mefloquine, Paediatrics and reproductive medicine, Pharmacology and pharmaceutical sciences, and Drug combinations
Although Casearia sylvestris Swartz is used in Brazilian folk medicine to treat obesity, no study has been conducted to evaluate the effects of this species in an experimental model of dyslipidemia and atherosclerosis. So, the aim of this... more
Although Casearia sylvestris Swartz is used in Brazilian folk medicine to treat obesity, no study has been conducted to evaluate the effects of this species in an experimental model of dyslipidemia and atherosclerosis. So, the aim of this study was to evaluate possible hypolipemiant and antiatherogenic activity of the methanolic extract obtained from Casearia sylvestris (MECS) using Swiss and C57BL/6 LDLr-null mice undergoing high fat diet (HFD). Dyslipidemia and atherogenesis were induced by the administration of commercial HFD for 4 weeks. The MECS was administered orally at doses of 250 and 500mg/kg, once a day, for two weeks, starting from the 2nd week of HFD. The gain in body weight and systolic blood pressure (SBP) were measured weekly over the four week study. At the end of the experiments the levels of triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and very low-density lipoprotein cholesterol (VLDL-C) were measured by colorimetric method. Aldosterone, vasopressin and angiotensin converting enzyme (ACE) activity were also evaluated in collected serum. The renal function, atherogenic index serum (AIS) and in vitro antiplatelet activity were investigated. Additionally, histopathological analyzes were performed to determine the intima-media thickness (IMT) and intima media ratio (IMR) in aorta samples. The HFD induced dyslipidemia and major structural changes in the aortic wall, including raising of the systolic blood pressure in LDLr-null mice. In addition, we observed an increase in lipid peroxidation accompanied by a reduction of serum nitrite. The treatment with MECS was able to prevent the increase of SBP, TC, LDL-C, VLDL-C and triglycerides levels and increase HDL-C in Swiss and LDLr-null mice. These effects were accompanied by a significant reduction in oxidative stress. Moreover, AIS, IMT and IMR were significantly reduced in MECS-treated mice, and the extract was able to reduce platelet aggregation in vitro. This study demonstrated that MECS reduces the serum lipids and oxidative stress when orally administered to Swiss and LDLr-null mice. In addition, it was able to prevent arterial thickening induced by HFD and to inhibit platelet aggregation in vitro.
Research Interests: Endocrinology, Complementary and Alternative Medicine, Plant Biology, Atherosclerosis, Ethnopharmacology, and 14 moreMedicine, Lipids, Internal Medicine, Platelet aggregation, Blood Pressure, Animals, Male, Lipid peroxidation, Plant extracts, Dyslipidemia, Knockout Mice, Plant Leaves, Pharmacology and pharmaceutical sciences, and Kidney function tests
Research Interests: Pharmacology, Complementary and Alternative Medicine, Traditional Medicine, Plant Biology, Biology, and 15 moreEthnopharmacology, Medicine, Spleen, Toxicity, Kidney, Liver, Female, Animals, Male, Plant extracts, Rats, Body Weight, Biological markers, Tropaeolum, and Pharmacology and pharmaceutical sciences
Research Interests: Chemistry, Complementary and Alternative Medicine, Ethnopharmacology, Medicinal Plants, Medicine, and 15 moreHypertension, Diuretics, Aldosterone, Nitric oxide, Animals, Male, Ethanol, Hydrochlorothiazide, Biological markers, Diuretic, Diuresis, Angiotensin Converting Enzyme Inhibitors, Bradykinin, epoprostenol, and Natriuresis
Research Interests: Pharmacology, Endocrinology, Complementary and Alternative Medicine, Biology, Ethnopharmacology, and 15 morePharmacognosy, Medicine, Humans, Diuretics, Animals, Male, Mode of action, Electrolytes, Diuretic, Diuresis, Furosemide, Creatinine, Indomethacin, excretion, and Pharmacology and pharmaceutical sciences
Artemisinins combination therapy (ACT) is the first choice therapy for falciparum malaria. Data on the safety of ACTs in pregnancy are limited and controversial and the use is not recommended on the first trimester. To evaluate the... more
Artemisinins combination therapy (ACT) is the first choice therapy for falciparum malaria. Data on the safety of ACTs in pregnancy are limited and controversial and the use is not recommended on the first trimester. To evaluate the effects of isolated and combined artesunate (AS)/mefloquine (MQ) on embryo rats, pregnant rats were treated orally with AS (15 and 40 mg/kg body weight (bwt)/day), MQ (30 and 80 mg/kg bwt/day) and AS/MQ (15/30 and 40/80 mg/kg bwt/day) on days 9–11 post coitum (pc). The dams were euthanized on day 12 pc and gestational and embryos histological parameters were evaluated. Embryolethality and histopathological anomalies were significant when AS was given alone or combined with MQ. Combination of AS and MQ did not enhance their toxicity compared to their separate administrations; on the other side, there was a reduction in the toxic effects of the AS when combined with MQ. Isolated MQ did not induce developmental toxicity.