- Medical Mycology, Antifungal Drugs, Azol-Resistance Candida Spp, Azole-Resistance Genes in Candida Albicans, Microbiology, Candida species, and 18 morePrincipal Component Analysis, Secondary Metabolites, Fusarium oxysporum, Aspergillus niger, Antifungal Activity, Ketoconazole, Alternaria alternata, Aspergillus flavus, Aspergillus fumigatus, Disk Diffusion Method, Penicillium citrinum, Extraction of Lichens Using Soxhlet Apparatus, Albugo candida, Fusarium roseum, Fusarium solani, Soxhalet extraction, Chemical and Green Synthesis of Silver Nano Particles, and Green Synthesis of nanoparticlesedit
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Superficial and cutaneous fungal infections (SCFIs) are an important public health problem and are common in tropical and subtropical countries. Pityriasis versicolor, dermatophytosis, erythrasma, onychomycosis, and otomycosis are the... more
Superficial and cutaneous fungal infections (SCFIs) are an important public health problem and are common in tropical and subtropical countries. Pityriasis versicolor, dermatophytosis, erythrasma, onychomycosis, and otomycosis are the major diseases associated with SCFIs. The aim of this study was to evaluate the prevalence and causative agents of dermatomycoses over a period of 10 years in Kerman province, southeast of Iran. A number of 1782 subjects, including 1096 females and 686 males, with cutaneous disorders in their skin, nail, and hair suspected to have SCFIs participated in this study. The collected specimens were examined using direct microscopy examination, staining, culture on specific media and PCR-RFLP technique. In total, 617 (34.62%) subjects had SCFIs, of whom 290 (47%) were female and 327 (53%) were male. Identified SCFIs included yeast infections, dermatophytosis, saprophyte onychomycosis, erythrasma, and otomycosis due to non-dermatophytic molds (NDMs). The highe...
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Vulvovaginal candidiasis (VVC) or vaginal candidiasis is a common fungal infection of the genitals causing inflammation, irritation, itching, and vaginal discharge. Common yeast infections are caused by the yeast species C. albicans.... more
Vulvovaginal candidiasis (VVC) or vaginal candidiasis is a common fungal infection of the genitals causing inflammation, irritation, itching, and vaginal discharge. Common yeast infections are caused by the yeast species C. albicans. However, there are other species of Candida such as C. dubliniensis which are considered as the causative agents of this infection. Hydrolytic enzymes such as proteinase and coagulase are known as virulence factors. The aim of this study was the molecular confirmation and differentiation of C. dubliniensis among C. albicans strains isolated from women with vulvovaginal candidiasis by PCR-restriction fragment length polymorphism (PCR-RFLP) and the evaluation of proteinase and coagulase activities. A total of 100 C. albicans strains isolated from women with vulvovaginal candidiasis referred to Shiraz medical clinics were enrolled in the study. All the isolates were primarily identified by conventional methods. PCR-RFLP method was used for the confirmation...
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In recent years, biosynthesis and the utilisation of silver nanoparticles (AgNPs) has become an interesting subject. In this study, the authors investigated the biosynthesis of AgNPs using Trifolium resupinatum (Persian clover) seed... more
In recent years, biosynthesis and the utilisation of silver nanoparticles (AgNPs) has become an interesting subject. In this study, the authors investigated the biosynthesis of AgNPs using Trifolium resupinatum (Persian clover) seed exudates. The characterisation of AgNPs were analysed using ultraviolet-visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infra-red spectroscopy. Also, antifungal efficacy of biogenic AgNPs against two important plant-pathogenic fungi (Rhizoctonia solani and Neofusicoccum Parvum) in vitro condition was evaluated. The XRD analysis showed that the AgNPs are crystalline in nature and have face-centred cubic geometry. TEM images revealed the spherical shape of the AgNPs with an average size of 17 nm. The synthesised AgNPs were formed at room temperature and kept stable for 4 months. The maximum distributions of the synthesised AgNPs were seen to range in size from 5 to 10 nm. The highest inhibition effect was observed against R. solani at 40 ppm concentration of AgNPs (94.1%) followed by N. parvum (84%). The results showed that the antifungal activity of AgNPs was dependent on the amounts of AgNPs. In conclusion, the AgNPs obtained from T. resupinatum seed exudate exhibit good antifungal activity against the pathogenic fungi R. solani and N. Parvum.
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... immunodeficiency syndrome (AIDS) (Anibal., 2010 ).Antifungal drug resistance is a big problem in the immunocompromised patients (Jabra-Rizk et ... Paula Cristina, de Cássia Orlandi Sardi Janaina, Peixoto Iza Teixeira Alves, de... more
... immunodeficiency syndrome (AIDS) (Anibal., 2010 ).Antifungal drug resistance is a big problem in the immunocompromised patients (Jabra-Rizk et ... Paula Cristina, de Cássia Orlandi Sardi Janaina, Peixoto Iza Teixeira Alves, de Carvalho Moraes Julianna Joanna, Höfling José ...
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Tinea imbricata is an uncommon dermatophytosis caused by the anthropophilic dermatophyte Trichophyton concentricum in endemic regions. In the present study, a 10-year-old girl was examined for tinea imbricata. Microscopic examination... more
Tinea imbricata is an uncommon dermatophytosis caused by the anthropophilic dermatophyte Trichophyton concentricum in endemic regions. In the present study, a 10-year-old girl was examined for tinea imbricata. Microscopic examination revealed the presence of hyaline, septate, ...
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Objective: There has been a growing interest in finding plants with biological active ingredients for medicinal application. Materials and Methods: Three colors of petals of Althaea officinalis (A. officinalis) flowers, i.e., pink,... more
Objective: There has been a growing interest in finding plants with biological active ingredients for medicinal application. Materials and Methods: Three colors of petals of Althaea officinalis (A. officinalis) flowers, i.e., pink, reddish pink, and white were examined for total antioxidant activity and flavonoids content. Results: The reddish pink flowers of A. officinalis have more antioxidant activity and the power of antioxidant activity was reddish pink > pink > white. Conclusion: Findings suggest that the dark color can serve as an indicator of antioxidant content of the plant. Flavonoid content was highest in white flower thus this result indicated that flowers with light color can be considered for medicinal uses.
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Drug resistance is a common phenomenon frequently observed in countries where leishmaniasis is endemic. Due to the production of the pteridine reductase enzyme (PTR1), drugs lose their efficacy, and consequently, the patient becomes... more
Drug resistance is a common phenomenon frequently observed in countries where leishmaniasis is endemic. Due to the production of the pteridine reductase enzyme (PTR1), drugs lose their efficacy, and consequently, the patient becomes unresponsive to treatment. This study aimed to compare the in vitro effect of meglumine antimoniate (MA) on non‐ healing Leishmania tropica isolates and on MA transfected non‐healing one to PTR1.
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Leishmaniasis counts as one of the most neglected tropical diseases. Despite the amount of research perceived in this field, no fully effective and approved vaccine against this disease is yet available in humans. In this study, LACK and... more
Leishmaniasis counts as one of the most neglected tropical diseases. Despite the amount of research perceived in this field, no fully effective and approved vaccine against this disease is yet available in humans. In this study, LACK and KMP11 antigens were constructed simultaneously by recombinant methods in prokaryotic and eukaryotic expression systems and were compared and assessed along with the CpG adjuvant in BALB/c mice. In the prokaryotic method, LACK and KMP11 protein gene sequences were synthesized in pET28a-TEV vector. In order to extract these two proteins after expression, His-tag and S-tag sequences were added to the constructs, respectively for LACK and KMP11. The pET28a-TEV-LACK/KMP11 construct was transformed into Escherichia coli, and the inserts were verified by Colony PCR. Pure proteins were verified by western blot, and groups of BALB/c mice were injected with the created prokaryotic recombinant proteins along with an ODN CpG adjuvant. In the eukaryotic method, ...
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At present, there are no efficacious vaccines or effective drugs against leishmaniasis; therefore new and innovative control methods are urgently required. One way to achieve this important goal is through using reverse genetic... more
At present, there are no efficacious vaccines or effective drugs against leishmaniasis; therefore new and innovative control methods are urgently required. One way to achieve this important goal is through using reverse genetic engineering to evaluate important enzymes, proteins and macromolecules. One of the most important enzymes for Glycosylphosphatidylinositol (GPI) biosynthetic pathways is GlcNAc-PI-deN-acetylase (GPI12). The molecular constructs were cloned in Escherichia coli strain Top 10 and confirmed by molecular methods and were transfected by electroporation into Leishmania major. We demonstrated eishmaniasis everse genetic engineering
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Tinea imbricata is an uncommon dermatophytosis caused by the anthropophilic dermatophyte Trichophyton concentricum in endemic regions. In the present study, a 10-year-old girl was examined for tinea imbricata. Microscopic examination... more
Tinea imbricata is an uncommon dermatophytosis caused by the anthropophilic dermatophyte Trichophyton concentricum in endemic regions. In the present study, a 10-year-old girl was examined for tinea imbricata. Microscopic examination revealed the presence of hyaline, septate, branching hyphae and its cultures on Sabouraud dextrose agar yielded T. concentricum. The patient responded to treatment with oral terbinafine 250mg/day topical clotrimazole (1% ointment), topical miconazole (2% cream) two times daily and potassium permanganate for daily washing for four weeks. In the present study, we reported the first case of tinea imbricate from Iran.
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Background Leishmaniasis is a serious health problem in some parts of the world. In spite of the many known leishmaniasis control measures, the disease has continued to increase in endemic areas, and no effective vaccine has been... more
Background Leishmaniasis is a serious health problem in some parts of the world. In spite of the many known leishmaniasis control measures, the disease has continued to increase in endemic areas, and no effective vaccine has been discovered. Methods In this study, Leishmania tarentulae was used as a living factory for the production of two LACK and KMP11 immunogenic antigens in the mice body, and safety profiles were investigated. The sequences of the KMP11 and LACK L. major antigens were synthesized in the pLEXSY-neo 2.1 plasmid and cloned into E. coli strain Top10, and after being linearized with the SwaI enzyme, they were transfected into the genome of L. tarentolae. The L. tarentolae-LACK/KMP11/EGFP in the stationary phase with CpG ODN as an adjuvant was used for vaccination in BALB/c mice. Vaccination was performed into the left footpad. Three weeks later, the booster was injected in the same manner. To examine the effectiveness of the injected vaccine, pathogenic L. major (MRH...
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Background and Purpose: Candida species are the common opportunistic pathogens during the course of human immunodeficiency virus (HIV) infection. Oropharyngeal candidiasis (OPC) is generally known as the initial sign of HIV infection. The... more
Background and Purpose: Candida species are the common opportunistic pathogens during the course of human immunodeficiency virus (HIV) infection. Oropharyngeal candidiasis (OPC) is generally known as the initial sign of HIV infection. The aim of this study was to compare demographic characteristics and frequency of Candida species between HIV/AIDS patients and HIV-free subjects in Kerman, southeast of Iran. Materials and Methods: This study was conducted on 143 samples collected from the oral cavity of 81 HIV/AIDS patients and 35 HIV-free subjects. The samples were cultured on Sabouraud dextrose agar and CHROMagar. The identification of Candida species was accomplished using the color of colony and polymerase chain reactionrestriction fragment length polymorphism. Results: According to the results, C. albicans (n=25, 69.14%) was the most prevalent species isolated from the HIV/AIDS patients, followed by C. glabrata (n=19, 23.46%). Other isolated species included C. parapsilosis (n=4...
is the most common species (sp.) isolated from fungal infections. Azole resistance in species has been considerably increased in the last decades. Given the toxicity of the antimicrobial drugs, resistance to antifungal agents, and drug... more
is the most common species (sp.) isolated from fungal infections. Azole resistance in species has been considerably increased in the last decades. Given the toxicity of the antimicrobial drugs, resistance to antifungal agents, and drug interactions, the identification of new antifungal agents seems essential. In this study, we assessed the antifungal effects of biogenic selenium nanoparticles on and determined the expression of and genes. Selenium nanoparticles were synthesized with sp. MSH-1. The ultrastructure of selenium nanoparticles was evaluated with a transmission electron microscope. The antifungal susceptibility test was performed according to the modified Clinical and Laboratory Standards Institute M27-A3 standard protocol. The expression levels of the and genes were analyzed using the quantitative real-time polymerase chain reaction (PCR) assay. The azole-resistant and wild type strains were inhibited by 100 and 70 µg/mL of selenium nanoparticle concentrations, respective...