Nano-Enabled Colorimetric Assay for the Detection of Paracoccidioides lutzii: Advancing Diagnostics with Nanotechnology
<p>Gold nanoparticle characterization: (<b>a</b>) morphology by TEM; (<b>b</b>) hydrodynamic size distribution and zeta potential value; (<b>c</b>) UV–visible spectrum.</p> "> Figure 2
<p>UV–visible spectra of AuNPs from colorimetric tests. The negative result is the red curve, the positive result is the blue curve, and control AuNPs are shown in black.</p> "> Figure 3
<p>UV–visible spectra of colorimetric test fitted by the Gaussian equation: (<b>a</b>) Gaussian area; (<b>b</b>) FWHM; (<b>c</b>) Gaussian center.</p> "> Figure 4
<p>Automatic discrimination of the colorimetric test as determined by Equation (1). The integrated areas of UV–visible spectra were defined by the regions 488–530 nm and 533–575 nm.</p> "> Figure 5
<p>Receiver operating characteristic curve of the colorimetric tests.</p> ">
Abstract
:1. Introduction
2. Materials and Methods
2.1. Nanoparticle Synthesis and Characterization
2.2. Paracoccidioide lutzii DNA Extraction
2.3. Colorimetric Tests Using Gold Nanoparticles and Data Processing
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Filho, O.O.C.; Cândido, M.A.; Ventura, A.; Morais, F.V.; Raniero, L. Nano-Enabled Colorimetric Assay for the Detection of Paracoccidioides lutzii: Advancing Diagnostics with Nanotechnology. J. Nanotheranostics 2024, 5, 75-83. https://doi.org/10.3390/jnt5030005
Filho OOC, Cândido MA, Ventura A, Morais FV, Raniero L. Nano-Enabled Colorimetric Assay for the Detection of Paracoccidioides lutzii: Advancing Diagnostics with Nanotechnology. Journal of Nanotheranostics. 2024; 5(3):75-83. https://doi.org/10.3390/jnt5030005
Chicago/Turabian StyleFilho, Olavo O. Comparato, Marcela A. Cândido, Aveline Ventura, Flavia V. Morais, and Leandro Raniero. 2024. "Nano-Enabled Colorimetric Assay for the Detection of Paracoccidioides lutzii: Advancing Diagnostics with Nanotechnology" Journal of Nanotheranostics 5, no. 3: 75-83. https://doi.org/10.3390/jnt5030005