Heat shock protein 90 localizes to the surface and augments virulence factors of Cryptococcus neoformans
Fig 7
Hsp90 plays a crucial role in intrinsic anidulafungin tolerance of Cryptococcus neoformans.
(a) In vitro antifungal susceptibility of C. neoformans to anidulafungin was evaluated at both 25°C and 37°C. Growth in presence of anidulafungin was seen even at high concentrations of the drug indicating robust intrinsic resistance. (b) MIC assay was performed with AF and Hsp90 inhibitor RAD at the concentrations indicated and incubated for 72 hours at 25°C. Growth was not found to be sensitive to combination of both drugs at 25°C. (c) Pharmacological inhibition of Hsp90 with RAD at 37°C reduces tolerance to AF at 37°C indicating additive interaction between these two drugs.