Protease Activated Receptor Signaling Is Required for African Trypanosome Traversal of Human Brain Microvascular Endothelial Cells
Figure 1
T. b. rhodesiense transmigration across HBMEC silenced for F2RL1 expression by RNAi.
Using laser capture microdissection 15 individual HBMEC expressing GFP in the PAR2-silenced and control siRNA cultures were collected and the RNA extracted. (A) Based on qRT-PCR using pre-designed F2RL1 primers and normalized to ACTB (β-actin transcripts), PAR-2 gene expression was reduced by over 95%. (B) T. b. rhodesiense IL1852 was incubated for 16h in triplicate with HBMEC monolayers silenced for PAR-2 expression (F2RL1 siRNA), with a matched scrambled siRNA control (control siRNA) construct, or with untreated HBMEC (no Rx control) and examined parasite transmigration.