Czech Journal of Genetics and Plant Breeding, 2023 (vol. 59), issue 3
Whole genome identification of CBF gene families and expression analysis in Vitis vinifera L.Original Paper
Xiang Fang, Yiling Lin, Chun Chen, Tariq Pervaiz, Xicheng Wang, Hefei Luo, Jinggui Fang, Lingfei Shangguan
Czech J. Genet. Plant Breed., 2023, 59(3):119-132 | DOI: 10.17221/82/2022-CJGPB 
The CBF (C-repeat binding factors) genes play important roles in response to abiotic stress and environmental changes. In the present study, a total of 18 CBF genes were identified from a grapevine. Their domains, phylogenetics, and collinearity were analysed. The results revealed, that 18 VviCBF genes were distributed on 10 chromosomes unevenly in the grape genome. Promoter data analysis showed that the CBF gene has many cis-acting elements related to plant growth and development, light response, hormone, and abiotic stress response. We found that six VviCBF genes including, VviCBF5, VviCBF13,...
A preliminary study on the root-knot nematode resistance of a cherry plum cultivar Mirabolano 29COriginal Paper
Jia Liu, Jin Zhu, Hongwen Li, Deyong Luo, Jialian Xie, Huajia Li, Shuo Liu, Yuping Zhang, Lijuan Chen, Xiaodou Xie, Dong Wang, Ke Li, Meiying Yao, Guowei Zhang
Czech J. Genet. Plant Breed., 2023, 59(3):133-140 | DOI: 10.17221/111/2022-CJGPB
Grafting is the most important means of breeding peach, plum, apricot, and other fruit trees, and the selection of the rootstock is crucial to the quality of the grafting and the yield of the products. The traditional commonly used peach rootstock is susceptible to root-knot nematode infections, resulting in a decreased yield, while a variety of cherry plum rootstocks, Mirabolano 29C, is resistant to root-knot nematode. In this study, root-knot nematode infection experiments on seedlings of traditional peach rootstocks and Mirabolano 29C confirmed that Mirabolano 29C was indeed more resistant to root-knot nematodes. At the same time, we compared the...
Effect of long-term storage on the change in the expression of selected Mal d 1 gene isoforms in the apple cultivar Opal®Original Paper
Ivona Žďárská, Radek Čmejla
Czech J. Genet. Plant Breed., 2023, 59(3):141-147 | DOI: 10.17221/102/2022-CJGPB
Apples are available all year round since they can be stored for long time. However, the expression of the major apple allergen Mal d 1 can increase in the fruit during storage and incease so the risk of allergies in sensitive consumers. Therefore, we studied the change in the expression of the Mal d 1 gene during storage in the modern cultivar UEB 32642 (known under the brand name Opal®) and the cultivar Gala Brookfield (cv. Gala) as a reference. The cultivars were stored under two different conditions, ultra-low oxygen (ULO) and standard cold conditions. The gene expression was evaluated both in fresh fruits and in fruits stored...
Assessment of genetic variation and population structure in Iraqi barley accessions using ISSR, CDDP, and SCoT markersOriginal Paper
Nawroz Tahir, Djshwar Lateef, Kamaran Rasul, Didar Rahim, Kamil Mustafa, Shokhan Sleman, Avin Mirza, Rebwar Aziz
Czech J. Genet. Plant Breed., 2023, 59(3):148-159 | DOI: 10.17221/112/2022-CJGPB
The objective of this study was to investigate the diversity of 59 accessions of barley using inter simple sequence repeat (ISSR), conserved DNA-derived polymorphism (CDDP), and start codon targeted (SCoT) markers. A total of 391 amplified polymorphic bands were generated using 44 ISSR, 9 CDDP, and 12 SCoT primers that produced 255, 35, and 101 polymorphic bands, respectively. The average values of gene diversity were 0.77, 0.67, and 0.81 for ISSR, CDDP, and SCoT markers, respectively. The mean values of polymorphism information content for ISSR, CDDP and SCoT markers were 0.74, 0.63, and 0.80 respectively. The discrimination power of the three approaches...
Complete chloroplast genome sequence and characteristics analysis of Qingda no.1 alfalfa (Medicago sativa L. cv. Qingda no.1)Original Paper
Yuling Ren, Yajun Ma, Xue Li, Xiaoan Li, Guozhu Yang, Ping Li
Czech J. Genet. Plant Breed., 2023, 59(3):160-168 | DOI: 10.17221/71/2022-CJGPB
Medicago sativa is the most widely cultivated forage legume and one of the most economically valuable crops throughout the world. Qingda no.1 (Medicago sativa L. cv. Qingda no.1) is an excellent alfalfa local variety with strong cold, drought and salt resistance in the three rivers source area of Qinghai. In this study, the whole chloroplast (cp) genome of Qingda no.1 was sequenced, assembled and its structure was analysed by the Illumina high-throughput sequencing technology. The results showed that the chloroplast genome of Qingda no.1 exhibits no obvious typical quadripartite structure; the total length of the chloroplast genome is...
Morpho-genetic characterization of diploid and tetraploid taro (Colocasia esculenta L. Schott) cv. Kaliurang – An Indonesian local cultivarOriginal Paper
Dyah Retno Wulandari, Andri Fadillah Martin, Tri Muji Ermayanti, Khalisa Aini Sinaga, Diah Ratnadewi
Czech J. Genet. Plant Breed., 2023, 59(3):169-175 | DOI: 10.17221/97/2022-CJGPB
Kaliurang is one of the local taro cultivars in Indonesia, potentially due to its high productivity and delicacy. This study analysed morpho-genetic variation between one diploid and three tetraploid Kaliurang taro clones from in vitro polyploid induction after plantlet acclimatization in the greenhouse for 6 weeks. Plants’ morphological characters were analysed using the unweighted pair group method with arithmetic mean (UPGMA) method based on 50 characters according to the taro descriptor from the International Plant Genetic Resources Institute (IPGRI). Furthermore, a morphometric characterization based on the Euclidean distance of 17...
Inheritance and allelism of brown midrib trait introgressed in agronomically promising backgrounds in pearl millet (Pennisetum glaucum (L.) R. Br.)Original Paper
Shashi Kumar Gupta*, Ponnaiah Govintharaj
Czech J. Genet. Plant Breed., 2023, 59(3):176-187 | DOI: 10.17221/93/2022-CJGPB
The lignin content of forage crops significantly affects the livestock’s forage intake and its dry matter digestibility. The brown midrib (bmr) trait is known for its association with a reduced lignin content and increased digestibility. Pearl millet inbreds having the bmr trait were derived from two populations ICMV bmr and WRajPop bmr via continued selfing up to 6–8 generations. The results from the crosses revealed the presence of the same single recessive gene that controlled the bmr in both the populations. The stable expression of the bmr gene in the new genetic background indicated that there...