Czech Journal of Genetics and Plant Breeding, 2021 (vol. 57), issue 1
QTL mapping of adult plant resistance to stripe rust in the Fundulea 900 × Thatcher RIL populationOriginal Paper
Xiaocui Yan, Huimin Zheng, Peipei Zhang, Gebrewahid Takele Weldu, Zaifeng Li, Daqun Liu
Czech J. Genet. Plant Breed., 2021, 57(1):1-8 | DOI: 10.17221/71/2020-CJGPB 
Wheat stripe rust, caused by Puccinia striiformis Westend. f.sp. tritici Eriks (Pst), is one of the most important diseases of bread wheat worldwide. Breeding resistant wheat cultivars is the most economical, effective and environmentally friendly way for controlling wheat stripe rust in China. The Romanian wheat line Fundulea 900 showed good resistance to wheat stripe rust at the adult stage. The present study aimed to map the quantitative trait loci (QTLs) for stripe rust resistance in 176 F2:6 recombinant inbred lines (RIL) derived from the cross of Fundulea 900 × Thatcher. The RIL population was phenotyped...
QTL identification for nine seed-related traits in Brassica juncea using a multiparent advanced generation intercross (MAGIC) populationOriginal Paper
Haifei Zhao, Wei Yan, Kunjiang Yu, Tianya Wang, Aimal Nawaz Khattak, Entang Tian
Czech J. Genet. Plant Breed., 2021, 57(1):9-18 | DOI: 10.17221/73/2020-CJGPB
Agronomic traits are usually determined by multiple quantitative trait loci (QTLs) that can have pleiotropic effects. A multiparent advanced generation intercross (MAGIC) population is well suited for genetically analysing the effects of multiple QTLs on the traits of interest because it contains more QTL alleles than a biparental population and can overcome the problem of confounding the population structure of the natural germplasm population. We previously developed the B. juncea MAGIC population, derived from eight B. juncea lines with great diversity in agronomic and quality traits. In this study, we show that the B. juncea...
Combining the null Kunitz trypsin inhibitor and yellow mosaic disease resistance in soybean (Glycine max (L.) Merrill)Original Paper
Sandeep Kaur Dhaliwal, Satwinder Kaur Dhillon, B.S. Gill, Asmita Sirari, Anita Rani, Roopan Dhillon
Czech J. Genet. Plant Breed., 2021, 57(1):19-25 | DOI: 10.17221/47/2020-CJGPB
For the wide adoption of soybean varieties, it is desirable, that they are Kunitz trypsin inhibitor (Kti) free and resistant to yellow mosaic disease (YMD). The soybean variety SL525 with YMD resistance was crossed with the YMD susceptible variety NRC101 with a null kti allele. The F5 progeny derived from the cross was screened with two simple sequence repeat (SSR) markers (satt409 and satt322) linked with the null kti allele and the YMD resistance, respectively, and one null kti allele-specific marker. The presence of both desirable traits was further confirmed with the phenotypic data...
Rabies virus glycoprotein produced in Nicotiana benthamiana is an immunogenic antigen in miceOriginal Paper
Youngmin Park, Hyangju Kang, Kyungmin Min, Nam Hyung Kim, Minhee Park, In-Ohk Ouh, Ha-Hyun Kim, Jae-Young Song, Dong-Kun Yang, Eun-Ju Sohn, Sangmin Lee
Czech J. Genet. Plant Breed., 2021, 57(1):26-35 | DOI: 10.17221/25/2020-CJGPB
Rabies remains an infectious disease among humans and animals, and requires the development of an effective vaccine essential to prevent rabies. Advances in molecular biology and biotechnology have led to the development and improvement of many rabies vaccines. Before the third-generation of the vaccine, rabies vaccines were based on the virus itself. Thus, even if effective, these vaccines may not be completely safe, resulting in a strong demand for the development of effective subunit vaccines that do not raise concerns about virus replication and infection in the host. This study investigated the ability of the glycoprotein of the rabies virus to...
Changes in the expression of CrFTA, the Catharanthus roseus farnesyltransferase α-subunit gene, in response to a Candidatus Liberibacter asiaticus infectionOriginal Paper
Ya Li, Qinhan Yu
Czech J. Genet. Plant Breed., 2021, 57(1):36-42 | DOI: 10.17221/13/2020-CJGPB
The farnesyltransferase α-subunit (FTA) may be involved in the regulation of defence responses against pathogens in plants. In this study, this gene was amplified from Catharanthus roseus (CrFTA gene). The cDNA was found to be 1 403 bp long, and encodes a putative protein of 331 amino acids that contains a conserved PPTA motif. The phylogenetic analysis showed that the sequence of CrFTA is the most similar to that from Coffea canephora. The qRT-PCR assays indicated that CrFTA is expressed in the leaves, stems, and roots. During a Candidatus Liberibacter asiaticus (Ca. L. asiaticus)...