TMED10

TMED10
Identifikatori
Aliasi	TMED10
Vanjski ID-jevi	OMIM: 605406 MGI: 3782198 HomoloGene: 4972 GeneCards: TMED10
Lokacija gena (čovjek)
Hrom.	Hromosom 14 (čovjek)
Kraj	75,176,612 bp
Obrazac RNK ekspresije
	; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• GO:0001948, GO:0016582 vezivanje za proteine; • syntaxin binding; • GO:0032403 protein-containing complex binding;
Ćelijska komponenta	• integral component of membrane; • Golđijev aparat; • endoplasmic reticulum membrane; • secretory granule membrane; • membrana; • intracellular membrane-bounded organelle; • Melanosom; • Golđijeva membrana; • cis-Golgi network; • ćelijska membrana; • transport vesicle membrane; • transport vesicle; • zymogen granule membrane; • Endoplazmatski retikulum; • ER to Golgi transport vesicle membrane; • trans-Golgi network transport vesicle; • endoplasmic reticulum-Golgi intermediate compartment membrane; • gamma-secretase complex; • GO:0016023 citoplazmatska vezikula; • COPI-coated vesicle; • endoplasmic reticulum-Golgi intermediate compartment; • COPII-coated ER to Golgi transport vesicle;
Biološki proces	• regulation of amyloid-beta formation; • Golgi organization; • Nefrogeneza; • COPI coating of Golgi vesicle; • vesicle targeting, to, from or within Golgi; • COPI-coated vesicle budding; • endoplasmic reticulum to Golgi vesicle-mediated transport; • COPII vesicle coating; • response to alkaloid; • protein complex oligomerization; • vesicle cargo loading; • protein transport; • regulated exocytosis; • intracellular protein transport; • vesicle-mediated transport; • retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum;
	Izvori:Amigo / QuickGO
Ortolozi
	10972
	100042773
	ENSG00000170348
	ENSMUSG00000114251
	P49755
	n/a
	NM_006827
	XM_036158265
	NP_006818
	n/a
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Emp24 transmembranski protein 10 sa domenom jeprotein koji je kosd ljudi kodiran genom TMED10.^[4]^[5]^[6]

Ovaj gen je član porodice EMP24 / GP25L/p24 i kodira protein sa GOLD domenom. Ovaj membranski protein tipa I lokaliziran je na plazmamembrani i Golgijevim cisternama i uključen je u promet vezikulskim proteinima. Protein je također član kompleksa heteromerne sekretaze i regulira aktivnost gama-sekretaznog kompleksa, bez utjecaja na njegovu aktivnost epsilon-sekretaze. Mutacije u ovom genu povezane su s ranom porodičnom Alzheimerovom bolešću. Ovaj gen ima pseudogen na hromosomu 8.^[6]

Analizom povezanosti, Sherrington et al. (1995) mapirali su gen TMP21 na hromosomu 14, regija 14q24.3.^[7]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 219 aminokiselina, a molekulska težina 24 976 Da.^[8]

10	20	30	40	50
MSGLSGPPAR	RGPFPLALLL	LFLLGPRLVL	AISFHLPINS	RKCLREEIHK
DLLVTGAYEI	SDQSGGAGGL	RSHLKITDSA	GHILYSKEDA	TKGKFAFTTE
DYDMFEVCFE	SKGTGRIPDQ	LVILDMKHGV	EAKNYEEIAK	VEKLKPLEVE
LRRLEDLSES	IVNDFAYMKK	REEEMRDTNE	STNTRVLYFS	IFSMFCLIGL
ATWQVFYLRR	FFKAKKLIE

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Kloniranje i ekspresija

Blum et al. (1996) identificirali su 21-kD protein mikrosomne membrane pacovske gušterače koji su označili kao Tmp21. Sondiranjem biblioteke cDNK u ljudskom mozgu s fragmentom pacovske sekvence izolirali su cDNK koja kodira ljudaski TMP21. Izvedeni unutarćelijski transmembranski protein 219-aminokiselinskog tipa I sadrži signalnu sekvencu i predviđeno je da se nalazi u lumenu endoplazmatskog retikuluma. Northern blot analiza otkrila je transkript TMP21 od 1,4 kb. Imunoblot analiza pokazala je da se pacovski protein Tmp21 eksprimira pretežno u mikrosomskoj frakciji acinarnih ćelija gušterače. Horer i dr. (1999) utvrdili su da je navodna izoforma TMP21, TMP21-II, neutralni pseudogen.^[9]^[10]

Funkcija gena

Chen et al. (2006) identificirali su TMP21 kao komponentu presenilinskog kompleksa koji različito regulira cijepanje gama-sekretaze, bez uticaja na aktivnost epsilon-sekretaze.^[11]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000170348 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Sherrington R, Rogaev EI, Liang Y, Rogaeva EA, Levesque G, Ikeda M, Chi H, Lin C, Li G, Holman K, et al. (Aug 1995). "Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease". Nature. 375 (6534): 754–60. doi:10.1038/375754a0. PMID 7596406.
^ Blum R, Feick P, Puype M, Vandekerckhove J, Klengel R, Nastainczyk W, Schulz I (Sep 1996). "Tmp21 and p24A, two type I proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking". J Biol Chem. 271 (29): 17183–9. doi:10.1074/jbc.271.29.17183. PMID 8663407.
^ ^a ^b "Entrez Gene: TMED10 transmembrane emp24-like trafficking protein 10 (yeast)".
^ Sherrington, R., Rogaev, E. I., Liang, Y., Rogaeva, E. A., Levesque, G., Ikeda, M., Chi, H., Lin, C., Li, G., Holman, K., Tsuda, T., Mar, L., Foncin, J.-F., Bruni, A. C., Montesi, M. P., Sorbi, S., Rainero, I., Pinessi, L., Nee, L., Chumakov, I., Pollen, D., Brookes, A., Sanseau, P., Polinsky, R. J., Wasco, W., Da Silva, H. A. R., Haines, J. L., Pericak-Vance, M. A., Tanzi, R. E., Roses, A. D., Fraser, P. E., Rommens, J. M., St George-Hyslop, P. H. Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease. Nature 375: 754-760, 1995. PubMed: 7596406
^ "UniProt, P49755". Pristupljeno 6. 2. 2017.
^ Blum, R., Feick, P., Puype, M., Vandekerckhove, J., Klengel, R., Nastainczyk, W., Schulz, I. Tmp21 and p24A, two type I proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking. J. Biol. Chem. 271: 17183-17189, 1996. PubMed: 8663407
^ Horer, J., Blum, R., Feick, P., Nastainczyk, W., Schulz, I. A comparative study of rat and human Tmp21 (p23) reveals the pseudogene-like features of human Tmp21-II. DNA Seq. 10: 121-126, 1999. PubMed: 10376215
^ Chen, F., Hasegawa, H., Schmitt-Ulms, G., Kawarai, T., Bohm, C., Katayama, T., Gu, Y., Sanjo, N., Glista, M., Rogaeva, E., Wakutani, Y., Pardossi-Piquard, R., Ruan, X., Tandon, A., Checler, F., Marambaud, P., Hansen, K., Westaway, D., St George-Hyslop, P., Fraser, P. TMP21 is a presenilin complex component that modulates gamma-secretase but not epsilon-secretase activity. Nature 440: 1208-1212, 2006. PubMed: 16641999

Dopunska literatura

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Gommel D, Orci L, Emig EM, et al. (1999). "p24 and p23, the major transmembrane proteins of COPI-coated transport vesicles, form hetero-oligomeric complexes and cycle between the organelles of the early secretory pathway". FEBS Lett. 447 (2–3): 179–85. doi:10.1016/S0014-5793(99)00246-X. PMID 10214941.
Hörer J, Blum R, Feick P, et al. (1999). "A comparative study of rat and human Tmp21 (p23) reveals the pseudogene-like features of human Tmp21-II". DNA Seq. 10 (2): 121–6. doi:10.3109/10425179909008429. PMID 10376215.
Wang H, Kazanietz MG (2002). "Chimaerins, novel non-protein kinase C phorbol ester receptors, associate with Tmp21-I (p23): evidence for a novel anchoring mechanism involving the chimaerin C1 domain". J. Biol. Chem. 277 (6): 4541–50. doi:10.1074/jbc.M107150200. PMID 11689559.
Majoul I, Straub M, Hell SW, et al. (2001). "KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET". Dev. Cell. 1 (1): 139–53. doi:10.1016/S1534-5807(01)00004-1. PMID 11703931.
Gommel DU, Memon AR, Heiss A, et al. (2002). "Recruitment to Golgi membranes of ADP-ribosylation factor 1 is mediated by the cytoplasmic domain of p23". EMBO J. 20 (23): 6751–60. doi:10.1093/emboj/20.23.6751. PMC 125325. PMID 11726511.
Barr FA, Preisinger C, Kopajtich R, Körner R (2002). "Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus". J. Cell Biol. 155 (6): 885–91. doi:10.1083/jcb.200108102. PMC 2150891. PMID 11739402.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Heilig R, Eckenberg R, Petit JL, et al. (2003). "The DNA sequence and analysis of human chromosome 14". Nature. 421 (6923): 601–7. doi:10.1038/nature01348. PMID 12508121.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Brandenberger R, Wei H, Zhang S, et al. (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197.
Breuza L, Halbeisen R, Jenö P, et al. (2004). "Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46". J. Biol. Chem. 279 (45): 47242–53. doi:10.1074/jbc.M406644200. PMID 15308636.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Chen F, Hasegawa H, Schmitt-Ulms G, et al. (2006). "TMP21 is a presenilin complex component that modulates gamma-secretase but not epsilon-secretase activity". Nature. 440 (7088): 1208–12. doi:10.1038/nature04667. PMID 16641999.
Chi A, Valencia JC, Hu ZZ, et al. (2007). "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes". J. Proteome Res. 5 (11): 3135–44. doi:10.1021/pr060363j. PMID 17081065.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000170348 - Ensembl, maj 2017

[2] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[3] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid7596406-4] Sherrington R, Rogaev EI, Liang Y, Rogaeva EA, Levesque G, Ikeda M, Chi H, Lin C, Li G, Holman K, et al. (Aug 1995). "Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease". Nature. 375 (6534): 754–60. doi:10.1038/375754a0. PMID 7596406.

[pmid8663407-5] Blum R, Feick P, Puype M, Vandekerckhove J, Klengel R, Nastainczyk W, Schulz I (Sep 1996). "Tmp21 and p24A, two type I proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking". J Biol Chem. 271 (29): 17183–9. doi:10.1074/jbc.271.29.17183. PMID 8663407.

[entrez-6] "Entrez Gene: TMED10 transmembrane emp24-like trafficking protein 10 (yeast)".

[7] Sherrington, R., Rogaev, E. I., Liang, Y., Rogaeva, E. A., Levesque, G., Ikeda, M., Chi, H., Lin, C., Li, G., Holman, K., Tsuda, T., Mar, L., Foncin, J.-F., Bruni, A. C., Montesi, M. P., Sorbi, S., Rainero, I., Pinessi, L., Nee, L., Chumakov, I., Pollen, D., Brookes, A., Sanseau, P., Polinsky, R. J., Wasco, W., Da Silva, H. A. R., Haines, J. L., Pericak-Vance, M. A., Tanzi, R. E., Roses, A. D., Fraser, P. E., Rommens, J. M., St George-Hyslop, P. H. Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease. Nature 375: 754-760, 1995. PubMed: 7596406

[UniProt-8] "UniProt, P49755". Pristupljeno 6. 2. 2017.

[9] Blum, R., Feick, P., Puype, M., Vandekerckhove, J., Klengel, R., Nastainczyk, W., Schulz, I. Tmp21 and p24A, two type I proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking. J. Biol. Chem. 271: 17183-17189, 1996. PubMed: 8663407

[10] Horer, J., Blum, R., Feick, P., Nastainczyk, W., Schulz, I. A comparative study of rat and human Tmp21 (p23) reveals the pseudogene-like features of human Tmp21-II. DNA Seq. 10: 121-126, 1999. PubMed: 10376215

[11] Chen, F., Hasegawa, H., Schmitt-Ulms, G., Kawarai, T., Bohm, C., Katayama, T., Gu, Y., Sanjo, N., Glista, M., Rogaeva, E., Wakutani, Y., Pardossi-Piquard, R., Ruan, X., Tandon, A., Checler, F., Marambaud, P., Hansen, K., Westaway, D., St George-Hyslop, P., Fraser, P. TMP21 is a presenilin complex component that modulates gamma-secretase but not epsilon-secretase activity. Nature 440: 1208-1212, 2006. PubMed: 16641999

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