BACTERIAL CONCRETE
Guided by:
Mr Rahul Ravi               HANSA
Assistant Professor         CE7A
Civil Department            YCE16CE035
YCET
                              1
CONTENTS
• Abstract
• Introduction
• Self Healing Bacterial Concrete
• Advantages Of Using Bacteria In Concrete
• Various Types of Bacteria Used In Concrete
• Viable Bacteria As Self Healing Agent
• Preparation Of Bacterial Concrete
• Mechanism Of Bacterial Concrete
                                               2
Contd..
• SEM Or Scanning Electron Microscopy Examination
• Advantages Of Bacterial Concrete
• Disadvantages Of Bacterial Concrete
• Cost Comparison Of Conventional And Bacterial Concrete
• Conclusion
• Reference
                                                           3
ABSTRACT
• Cracks in concrete are inevitable and are one of the inherent weakness of
  concrete.
• To remediate this type of failure of cracks and fissures, an approach of
  using biomineralization in concrete has evolved in recent years.
• As an aftermath of this approach, a type of concrete is developed in which
  bacteria is incorporated into concrete.
• To incorporate living matter into the structural material is in itself a great
  innovation.
                                                                              4
INTRODUCTION
• Cracking of concrete is an inevitable phenomenon related to durability.
• Repairing of existing cracks has been a subject of research for many
  years.
• In recent years, a bacteria based self healing concrete is being developed
  to extend the service life.
• Synthetic polymers such as epoxy treatment are currently being used as
  filling agents .
• The use of a biological repair technique or microbiologically induced
  calcite precipitation technique (MICP) in concrete is focused.
                                                                      5
SELF HEALING BACTERIAL CONCRETE
• Micro cracks with widths typically in the range of 0.05 to 0.1mm have
  been observed to become completely sealed autogenously.
• The mechanism of this autogenously healing is due to secondary
  hydration of non or partially reacted cement particles in the concrete
  matrix.
• Recent research has shown that bacteria can actually be a useful tool to
  repair cracks.
• Spore forming bacteria genus Bacillus, supplied with a calcium based
  nutrient are incorporated into the concrete.
                                                                      6
Contd..
• The bacteria can act as catalyst for the metabolic conversion of a suitable
  organic or inorganic component to produce the filler material.
• The filler material could be bio minerals such as calcite.
• These minerals are relatively dense and can block cracks, and thus hamper
  ingress of water efficiently.
                                                                          7
 ADVANTAGES OF USING BACTERIA IN
 CONCRETE
• Around five per cent of all man made carbon dioxide emissions are from
  the production of concrete.
• Finding a way of prolonging the lifespan of existing structures means we
  could reduce this environmental impact.
• Bacteria in concrete fills the crack in an efficient period of time so that the
  life period of a concrete structure can be expected over 200 years.
• Prevents the use of cement in future used as a maintenance structure by
  drilling and grouting process ,so in this way ,less use of cement can be
  seen .
                                                                          8
VARIOUS TYPES OF BACTERIA USED IN CONCRETE
 There are various types of bacteria used in construction area
 · Bacillus pasteurii
 · Bacillus sphaericus
 · Bacillus subtilis
 · Bacillus cohnii
 · Bacillus balodurans
 · Bacillus pseudofirmus
                                                                 9
VIABLE BACTERIA AS SELF HEALING AGENT
• For effective self healing, both bacteria and a bio-cement precursor
  compound should be integrated in the material matrix.
• When bacterial spores were directly added to the concrete mixture
  their lifetime appeared to be limited to one-two months.
• Another concern is whether direct addition of precursor compounds to
  the concrete mixture will not result in unwanted loss of other concrete
  properties.
• It was found that yeast extract, peptone and calcium acetate resulted in
  a dramatic decrease of compressive strength.
                                                                             10
Contd..
• Calcium lactate comprised to be an exception as it resulted in 10%
  increase in compressive strength.
• Protection of bacterial spores inside porous expanded clay particles, before
  addition to the concrete mixture.
• In this manner, their long term viability as observed in dry state when not
  embedded in concrete is maintained.
                                                                         11
PREPARATION OF BACTERIAL CONCRETE
Bacterial concrete can be prepared in two ways:
• By direct application
   By the method of direct application bacterial spores and calcium
    lactate are added directly while making the concrete and mixed.
• By encapsulation
   By encapsulation method the bacteria and it’s food , calcium lactate,
    are placed inside treated clay pellets and concrete is made.
                                                                    12
 MECHANISM OF BACTERIAL CONCRETE
• When a concrete structure is damaged and water starts to seep through the
  cracks that appear in the concrete , the spores of the bacteria germinate.
• Having been activated, the bacteria starts to feed on the calcium lactate.
• As the bacteria feeds, oxygen is consumed and the soluble calcium lactate
  is converted to insoluble limestone.
• The limestone solidifies on the cracked surface thereby sealing it up.
• It mimics the process by which bone fractures in the human body are
  naturally healed by osteoblast cells that mineralize to re-form bones
                                                                      13
Contd..
• Ureolytic bacteria such as Bacillus subtilis is able to precipitate calcium
  carbonate by converting urea into ammonium and carbonate.
• One mol of urea is hydrolysed intracellularly to one mol of ammonia and
  one mol of carbamic acid.
• Carbamic acid spontaneously hydrolysis to form additional one mol of
  ammonia and carbonic acid.
• These products equilibrate in water to form bicarbonate, one mol of
  ammonium and hydroxide ions.
                                                                         14
15
Figure 1: Calcite precipitation by bacterial cell   16
Figure 2: Concrete crack before healing and after healing
                                                            17
SEM OR SCANNING ELECTRON MICROSCOPY
EXAMINATION
• Bacteria with microbial calcite precipitation in the crack sample
  was examined in SEM .
• The sample showed the presence of calcite crystals grown all
  over the surface of the crack.
• It also showed the presence of Bacillus pasteurii bacteria.
• This is the evidence that suggests microbial remediation
  properties of bacterial concrete.
                                                                      18
ADVANTAGES OF BACTERIAL CONCRETE
• Microbial concrete in crack remediation
• Improvement in compressive strength of concrete
• Better resistance towards freeze-thaw attack
• Reduction in permeability of concrete
• Reduction in corrosion of reinforced concrete
                                                    19
DISADVANTAGES OF BACTERIAL
CONCRETE
• Cost of bacterial concrete is double than conventional concrete
• The clay pellets holding the self-healing agent comprise 20%
  of the volume of the concrete.
• Design of mix concrete with bacteria is not available in IS code.
• Investigation of calcite precipitate is costly
                                                                  20
COST COMPARISON OF CONVENTIONAL AND
BACTERIAL CONCRETE
• The cost of self-healing concrete is about double that of conventional
  concrete
• Self-healing concrete would only be a viable product for certain civil
  engineering structures.
• If produced on an industrial scale it is thought that the self- healing
  concrete could come down in cost considerably.
• If the life of the structure can be extended by 30%, the doubling in
  the cost of the actual concrete would still save a lot of money in the
  longer term.
                                                                      21
Contd..
• A second self-healing agent that will be much cheaper and also would
  result in much stronger concrete is being developed.
• Presently the majority of the extra cost comes from the calcium lactate
  which is very expensive.
• The process of embedding the bacteria and nutrients into the pellets is
  also expensive because it involves a vacuum technique.
• A sugar-based food nutrient would potentially bring down the cost
                                                                     22
CONCLUSION
• Bacterial concrete technology has proved to be better because of
  its eco- friendly nature.
• Work of various researchers has improved our understanding on
  the possibilities and limitations of biotechnological applications on
  building materials.
• Enhancement of compressive strength, reduction in permeability,
  water absorption, reinforced corrosion.
                                                                      23
Contd..
• In bacterial concrete interconnectivity of pores is disturbed due to
  plugging of pores with calcite crystals.
• This will soon provide the basis for high quality structures that will
  be cost effective and environmentally safe.
• More work is required to improve the feasibility of this technology
  from both an economical and practical viewpoints.
                                                                           24
REFERENCES
• Sakina Najmuddin Saifee, Divya Maheshbhai Lad, Jayesh Rameshbhai
  Juremalani. Critical appraisal on Bacterial Concrete, IJRDO-Journal Of
  Mechanical And Civil Engineering, ISSN: 2456-1479, Volume-1, Issue-
  3.
• Meera C.M, Dr.Subha V. Strength and Durability assessment Of
  Bacteria Based Self-Healing Concrete. IOSR Journal of Mechanical and
  Civil Engineering, e-ISSN: 2278-1684.
• Ravindranatha, N. Kannan, Likhit M. L .Self healing material bacterial
  concrete, International Journal of Research in Engineering and
  Technology, Volume: 03,Special Issue: 03.
                                                                    25
• A.T.Manikandan, A.Padmavathi. An Experimental Investigation
  on Improvement of Concrete
• Serviceability by using Bacterial Mineral Precipitation Volume II,
  Issue III.
• Jagadeesha Kumar B G, R Prabhakara, Pushpa H. Effect Of
  Bacterial Calcite Precipitation On Compressive Strength Of
  Mortar Cube, International Journal of Engineering and Advanced
  Technology (IJEAT), ISSN: 2249 – 8958, Volume-2, Issue-3,
  February 2013,PP 486-491
• RA. B. Depaa and T. Felix Kala Experimental Investigation of
  Self Healing Behavior of Concrete using Silica Fume and GGBFS
  as Mineral Admixtures Indian Journal of Science and Technology,
  Vol 8(36), DOI: 0.17485/ijst/2015/v8i36/87644.
                                                                  26