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The document outlines an experiment involving the drawing and analysis of plant stem and root sections. It includes instructions for drawing xylem vessels and calculating the width of vascular tissue using an eyepiece graticule. Additionally, it requires the preparation of a table to compare observable differences between the stem and root specimens.

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257 views4 pages

Solved - Exp 14

The document outlines an experiment involving the drawing and analysis of plant stem and root sections. It includes instructions for drawing xylem vessels and calculating the width of vascular tissue using an eyepiece graticule. Additionally, it requires the preparation of a table to compare observable differences between the stem and root specimens.

Uploaded by

alekspydie
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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EXPERIMENT NO.

14

2 N1 is a slide of a stained transverse section through a plant stem.

You are not expected to be familiar with this specimen.

Use a sharp pencil for drawing.

You are expected to draw the correct shape and proportions of the different tissues.

(a) (i) Draw a large plan diagram of the sector of the stem on N1, shown by the shaded area in
Fig. 2.1.

Use one ruled label line and label to identify the xylem.

draw this sector

Fig. 2.1

-
& =
glem
Op
=

[5]

9700/36/O/N/19

1
(ii) Select four adjacent, touching xylem vessels.

Each vessel must touch at least two other cells.

Make a large drawing of this group of four touching xylem vessels.

Use one ruled label line and label to identify the cell wall of one vessel.

G
8
LA
cell wall

J
O [5]

2
(b) Fig. 2.2 is a photomicrograph of a stained transverse section through the root of a different
type of plant.

You are not expected to be familiar with this specimen.

An eyepiece graticule scale is shown on Fig. 2.2.

The calibration of the eyepiece graticule scale is:

1 eyepiece graticule division = 24.6 μm

0 10 20 30
4 29
X Y

25g

Fig. 2.2

3
(i) Use the calibration of the eyepiece graticule on Fig. 2.2 to calculate the actual width of
the vascular tissue, shown by line X–Y.

Show all the steps in your working and use appropriate units.

x y
25 epg
- =

= 25 x 24 6
.
m

615 Mm

615 um
actual width of vascular tissue = ......................................................... [4]

(ii) Prepare an appropriate table so that it is suitable for you to record the observable
differences between the stem on N1 and the root in Fig. 2.2.

Record the observable differences in your table.

7
Feature Slide N1
Fig 22
.

Number
Vascular
many
One

centre
Position
of
bundles
Vascular around
edge
in

Shape of sylem tissue oval cross


shaped

Epidermis smooth rough

three)
Carry
[4]

[Total: 18]

9700/36/O/N/19
4

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