EXPERIMENT NO.
12
2 L1 is a slide of a stained transverse section through a plant leaf.
You are not expected to be familiar with this specimen.
You are required to:
• use the eyepiece graticule to measure part of a leaf and a vascular bundle
• use these measurements to calculate the depth of the vascular bundle as a percentage of the
depth of the leaf
• draw a plan diagram of part of the leaf.
(a) The eyepiece graticule in the microscope can be used to measure different tissues.
Select a part of the leaf on L1 which shows the widest part of the leaf (mid-rib) shown by Y in
Fig. 2.1.
Fig. 2.1
(i) Use the eyepiece graticule in the microscope to measure:
• the depth of the leaf at Y
• the depth of the vascular bundle at Y.
depth of leaf 98
..................... eyepiece graticule units
depth of vascular bundle 19
..................... eyepiece graticule units [1]
(ii) Use the measurements from (a)(i) to calculate the depth of the vascular bundle as a
percentage of the depth of the leaf.
You may lose marks if you do not show your working.
98
%
- 10
-
19
1900
-
98
19
. 4
answer = .......................................................% [2]
Use a sharp pencil for drawing.
(iii) Use the measurements from (a)(i) to help you draw a large plan diagram of the section
of the leaf shown by the shaded area in Fig. 2.2.
draw this section
Fig. 2.2
You are expected to draw the correct shape and proportions of the different tissues.
Use one ruled label line and label to identify the vascular bundle.
-
--
-
-
&&
C
&
-
u
We Vascular Bundle
[5]
(iv) Observe the vascular bundle in the central part of the leaf on L1.
Select one group of four adjacent (touching) xylem vessel elements. Each element must
touch at least one of the other elements.
Make a large drawing of this group of four xylem vessel elements.
Use one ruled label line and label to identify the lumen of one xylem vessel element.
B
& Lumen
[5]
(b) Fig. 2.3 is a photomicrograph of a stained transverse section through a different type of leaf.
You are not expected to be familiar with this specimen.
Fig. 2.3
Prepare the space below so that it is suitable for you to record the observable differences
between the leaf sections on L1 and in Fig. 2.3.
Record your observations in the space you have prepared.
Feature Slide 21
Fig . 2 3
.
Sunken Stomata present absent
Trichomes Present around absent
Stomata
Air absent Present around
spaces
vascular bundle
[4]
[Total: 17]
