SCOPE OF WORK

TECHNICAL SPECIFICATIONS OF ITEM

FOR ERECTION OF
“TISSUE CULTURE LABORATORY”
[Area Required (2.0 Acre)]

S. N. Equipment Specification Quantity

(Unit/Area)
1. Infrastructure
Laboratory Area ANNEXTURE-1
Sterilize and Semi- • Media Preparation & Chemical Storage Room Covered
sterilize Area • Sterilization Room area
• Buffer/Change Room (Approx.)
• Media Storage Room 450 Sq.M.
• Inoculation Room
• Culture Room
• Bottle Storage Room
• Store room
• Packing room/ pre-hardening area
(Annexure-1)
I. Non-Sterilize Area • Bottle Washing Area
• Hand & Leg wash Area
• Wash Room
2. Primary Hardening Primary Hardening Unit / Green House (Annexure- 2A) 1500 Sq.M.
I. Facility
II. Secondary Secondary hardening unit (Annexure- 2B) 4000 Sq.M.
Hardening Facility
3. Glassware and Plastic-ware
I. Bottle with caps Glass bottle 300ml capacity with wide mouth culture 80000 Nos.
bottles with autoclavable plastic lid
II. Growth Room Tray Trays needed for keeping the bottles 1250 Nos.
III. Plastic/ Glass All lab use size containing 5 No. each 2set
Beakers
IV. All glass measuring All lab use size containing 1 No. each 1 set
equipment
V. All Plastic All lab use size containing 1 No. each 1 set
measuring
equipment
VI. Conical flask Capacity of different size litres: 3 each 1 set

VII. Test tube Glass test tube 200 Nos.

VIII. Petri plates Glass Petri plates, Sterile Dia: 90mm x Hight: 14mm 50 Nos.

Glass Petri plates, Dia: 60mm x Hight: 14mm 10 Nos.

Glass Petri plates, Sterile Dia: 120mm x Hight: 14mm 10 Nos.

 IX. Glass reagent Capacity 100ml, 250ml, 500ml, 1000ml, 2000ml- 5each 1 set
bottles with screw
cap
X. Amber coloured Capacity 100ml, 250ml, 500ml, 1000ml, 2000ml- 5each 1 set
glass reagent
bottles
XI. Volumetric flask Capacity 100ml, 250ml, 500ml, 1000ml- 2each 1 set
XII. Glass test tubes 60 ml 1000 Nos

XIII. Multipurpose Plastic, perforated tray 50 Nos

utility tray
XIV. Multipurpose Plastic tray 50 Nos
utility tray
4 Metalwares and
Labwares
I. Laboratory Stainless steel, 6” spatula, pack of 6 6 set
Spatula, 6” S.S.
II. Long forcep Size 12”, stainless steel, pack of 6 6 set
III. Scalpel holder Stainless steel 20 Nos
IV. Spirit lamp Stainless steel 12 Nos
V. Scissor Stainless steel,”6 12 Nos
VI. Scalpel blades Stainless steel, No. 11, pack of 30 2 set
VII. Beaker tongs Stainless steel with plastic grip 3 set
5 Furniture and
Office supplies
I. Lab furniture Executive Chair: 10 Nos 1 set
Computer Chair: 5 Nos
Computer Table: 5 Nos
Laminar Chair: 24 Nos
Visitor Chair: 20 Nos
Office Table: 3 Nos
Shoe Rack: 4 Nos
Writing Board: 4 Nos
Conference Table Set: 1 Nos
Misc: 1 Nos
6. Miscellaneous
other items
I. Apron, cap mask & Lab Apron- 20 Nos. 20 Nos.
gloves Caps- 20 Nos. Each
Mask-20 Packet.
Gloves- 20 Packet.
II. Slipper Standard Rubber slipper 15 Nos.
III. CCTV Full HD for 16 Channel hd Monitor -1 No. 1 Set
monitoring of lab 1.3MP. Camera - 16 Nos.
4TB Surveillance HDD- 1 No.
Power Supply 05A (Linias)- 3 No.
3+ solid copper wires- 1No.
Bnc Connector-32Nos.
Dc connector- 16 nos.
Rack-1No.
 Installation- 16 Camera
IV. Computer and PC with 21” LCD monitor, (Dell/Lenovo/ HP/ Acer make) 1 Set
Printer are built specifically for the unique needs of small
businesses and are ready to be customized with the features
and software you want. The Computer is a powerful,
secure and reliable desktop solution that’s optimized to
meet your high performance business demands.

Printer:
HP laser Jet Colored printer
V. Water Tank PVC Tank capacity 20000 Ltrs. with tank platform 1 No.

6. Equipment:
S.No. Items Specifications Qty.
1. Autoclave (Fully Horizontal, front-loading autoclave 1 No.
Automatic) HORIZONTAL BENCH-TOP AUTOCLAVES
Horizontal Bench-top SYSTEC DSERIES
Autoclaves (Front • For all laboratory applications even for sophisticated
Loading) 1200 Ltr. state-of-the-art sterilization processes. With all
possibilities to add additional options for process
optimization to enable valid a table sterilization to be
carried out.
• Corrosion resistant pressure vessels made of stainless
steel 1.457 AISI 316 Ti, with the housing and support
frameworks made of steel as well.
• Temperature sensor in addition to an electronic
pressure sensor to monitor both the pressure and
temperature
• Front-loading bench top Horizontal autoclaves with
150 liters chamber volume is distinguished by its
small footprint and high chamber capacity. Result:
optimal loading capacity with most standard media
bottles and Erlenmeyer flasks.
• The door as well as various other elements of the
housing and vessel are built using stainless steel and
clad in an aesthetically pleasing front cover is made of
insulated plastic that is highly resistant to heat. The
same material is used for the control panel, display as
well as parts of the control processing system. This
prevents operators from coming into contact with
heated surfaces.

2. Electronic Weighing Weighing capacity: 220g 1 No.

Balance (Readability Readability: 0.1mg
0.1mg) Repeatability: 0.1mg
Linearity: 0.2mg
Weighing pan size: Ø90mm
 Weighing chamber height: 209mm
Net weight, approx. : 4.9kg
Dimensions, D x W x H : 360 x 216 x 320mm; 14.1 x 8.5
x 12.6Inch
3. Electronic Weighing Weighing capacity : 310g 2 Nos.
Balance (Readability Readability: 1mg
1.0mg) Repeatability: 1mg
Linearity: 2mg
Weighing pan size: Ø120mm
Weighing chamber height: 209mm
Net weight, approx.: 4.9kg
Dimensions, D x W x H : 360 x 216 x 320mm; 14.1 x 8.5
x 12.6Inch

4. pH meter cum Eutech pH 700 Meter: with pH Electrode (ECFC 5 No.

conductivity meter 7252101B), ATC Probe (ECPHTEMPB01P) Integral
(Digital) Electrode Holder & 100/240 VAC Adapter

pH :
Range : -1.99 to 16.00 pH
Resolution: 0.01pH
Accuracy: ±0.002
Cal. Points: Up to 5 points
Buffer Sets: USA, NIST

ORP:
Range: ±1999 mV
Rel. mV Range: ±1999 mV
Resolution: 0.1 mV (±199.9 mV) / 1 mV (beyond)
Accuracy: ±0.2 mV (±199.9 mV) / ±2 mV (beyond)

Temperature:
Range (meter): 0.0 to 100.0 ºC / 32.0 to 212.0 ºF
Resolution: 0.1 ºC / 0.1 ºF
Accuracy: ±0.5 ºC / ±0.9 ºF
Coefficient (Per ºC) Compensation Type & Range:
Automatic or Manual (0 to 100 ºC / 32 to 212 ºF)
Calibration: Off set in 0.1 º increments; offset range: ± 5
ºC / 9 ºF
5. Laminar Airflow/Bio Thermo Scientific Class II Type A2 4 Feet Biological 9 Nos.
safety Cabinet Class- Safety Cabinet cum Laminar Air Flow with 10 Inches
II (size 5-6 ft) Work Aperture and Smart coat Interiors
TECHNICAL SPECIFICATIONS
Horizontal type Laminar Airflow
Package: Includes cabinet, manual adjustable height
stand, factory installed UV light and one set of armrests
ERGONOMICS AND UTILITIES
Lighting Power : >120 Fc (1200 Lux)
Sound Level: < 62 dB(A)
Dimensions Exterior without Stand : 6’ x 2’ x 2’
Height of Stand: Min 750 mm Max 950mm
 External Cabinet Height on Stand: 2320 mm Min; 2520
Max
Interior: 1200 W x 780 H x 630 D mm
Working Height of Front Window: 10“(254mm)
Max. Opening of front window: 21 “ (535 mm)
ELECTRICAL DATA Voltage : 230 V
Frequency: 50 Hz
FILTER SPECIFICATIONS: H14 HEPA EN1822,
99.995% MPPS
6. Water Purification RO System 1000 LPH with stand & other accessories 1 Nos.
System
7. Glass Bead Sterilizer These are incorporated with a thermostat which maintains 9 Nos. (1
the set temperature with an accuracy of + 1 of the preset for each
temperature range. laminar)
Crucible (Brass) size:2”dia x 5½” Depth
Preset Temp. Range: 245°C – 265°C
Initial Stabilization Time: 30 Minutes (including. of Glass
Beads) 250W Single Phase

8 Air curtain MS powder coated body, Slick in design, with auto ON/ 3 Nos
OFF switch can save upto 80% of Electricity. Max. air
velocity at nozzle: 21m/s, Noise Level-DB 50-70, No.of
Blowers-3, No. of Motors-2, No-f shaft-1 Double,
Voltage- 350w-605w, Phase Single RPM(Max./Min.)-
2700/2100, Blower Type-ABS, cfm-825-1490, Insect/
Dust rejection-75%
9 Photoperiodic Analog Photoperi 24 hrs repeat cycle min ON/ OFF period 4 Nos
morphogen controller with NICAD battery auto recharging. Powered output can
drive 110 tibe lights 40 watt directly. Thermal safety
16amp inbuilt. Accuracy + 8 sec/day. Operating viltage
220 volt AC
10 Sequential Timer For operating two air conditioners of 1.5 ton each, Output-
Operates two AC upto 1.5 ton each, Min. on/off cycle-30
min.
11 Autoclave (Vertical • Double walled. 1 Nos.
Type) One • Inner outer chamber stainless steel.
• Lid is made of thick stainless steel.
• Pressure adjustable between 5 to 22 P.S.I.
• ISI mark heating element, which are easily replaceable
and heat the water to desired temperature and pressure.
• Pressure is indicated by pressure gauge supplied
complete with stainless steel perforated.
• Basket cord plug to work on 220v +/- 9% 50Hz, single
phase.
• Pressure control device (PIEZOSTAT).
• Microprocessor based controller with programmable
features with PID Display.
• PV & SV 16X2 character LCD display, 4 program
keys, one start/ stop key, pt-100 3wire.
• Sensor, automatic soak, purge, exhaust setting with
high alarm set. Automatic Timer.
• Size of inner chamber- 450x600 mm.

12 Vertical Distillation • Capacity 5 Ltr./hr 1 Nos

Apparatus • Quartz distiller, Demountable boiler
• Panel box and stand to accommodate regulator
clamp etc.
• Quality of distillate – pyrogen free, PH-6.9 –
conductivity
• Distilled water should be heavy metal, salts,
pyrogen and iron free.
• Specific Conductivity at 25 deg C less than 0.4 X
10.6S/cm Glass material (or Chemical inert
material).
• Equipment should be thermal shock proof.
13 Stereo Zoom Zoom body:7:1 zoom microscope body, Galilean Optics. 1 No.
Microscopes with Zoom magnification variation: Left/right optical axis
Camera parallel method. Zoom magnification indication: 0.8, 1,
1.25, 1.6, 2, 2.5, 3.2, 4, 5, 5.6.
Zoom drive: Horizontal knob system. Click stop for each
zoom magnification: ON-OFF switching possible.
Objective mounting: Screw mounting into thread.- 1 no.
Observation Tube:30 degrees inclined trinocular
observation tube with 2 step light path selection: 100:0 &
50:50 - 1no.

Digital Colour Camera:

5.1 Mega pixels digital camera, sensor size: 1/2.5",
binning: 2x2, 4x4, Spectral Range: 380-650 nm with IR
filter, integrated metal alloy housing, USB interface.
Compatible adaptor included – 1 no.
14 Bottle Washing Unit Bottle washing unit- High-performance Cleaning 1 No.
Outstanding fast cycle and high-
 Performance cleaning results from efficient direct injection
washing. High performance pump ensure high flow rate of
water. Efficient water spray pressure bring about better and
Faster cleaning. Water, liquid detergent and distilled water
automatically injected by program washing cycle. Safety
Door Lock Door is automatically locked for user safety
when washing cycle starts. Compact, Robust Design &
Efficient Construction Compact design for space saving
and maximize working capacity in laboratory. Double
layer structure ensures soundproof and heatproof during
operation.
Built-in-steam condenser prevents steam to enter into the
laboratory. Telescopic sliding rails ensures safe and easy
to load and unload.

15. Peristaltic pump for Peristaltic pump for media dispensing Specifications Flow 2 Nos.
media dispensing rates 0.7 to 6000 ml/min Dispensing volume.

External control start/stop control, direction

control, flow rates control (0 - 5V ,0 -10V, 4-20 mA , 0-
10KHz optional)
Output interface start/stop, direction output and 0-10KHz
frequency output Communication interface RS 485 Power
AC 176 - 264V, 50/60 Hz Power consumption <140W,
Operating condition Temperature 0 to 40°C, Relative
humidity < 80%
16. Magnetic Stirrer with 18.4x18.4cm 2 Nos.
Hot Plate Speed Range : 50 to 1500rpm
Temperature Range (Metric) : 300°C (Max Temperature)
Top Plate Material : Aluminum Surface Area (Metric)
Heating : 18.4 x 18.4cm
CE Shipping Weight (Metric) : 5.5kg
Stirring Range : 50 to 1,500rpm
Dimensions (L x W x H) : 13 x 8.2 x 3.8 in. (33 x 20.8 x
9.7cm)
Max. Temperature (Metric) : 300°C
Electrical Requirements:230V 50/60Hz
17. Media Cooking Unit Media preparation Vessel 1 No.
Capacity- 25 liter
18 Growth Room Racks Growth room Racks with cool LED 18 Nos.
(18 Racks / Room) Light
with cool LED Light Light weight structure. Size (HxLxB):
(Size 8 x 4 x 8-9) 8x4x 8-9 feet. 5 shelves in each rack and fitted with at least
LED light. Racks are fitted with wheel for easy movement.
19 Trolleys SS Trolleys for Material Movement 6 Nos.
914 x 610 x 750 mm ( W D H ) * MOC – SS 304 * Matt
Finish * Wheels For Movement * Rack For Storage And
Movement
20 Diesel Gen set (110 A. DIESEL ENGINE: 110 KVA, 4 cylinders, inline, 4 1 No.
KVA) stroke cycles, Liquid cooled, developing 126.00 BHP at
1500.00 RPM under NTP conditions of BS: 6. The engine
shall be provided with electrical starting arrangement &
 shall give the electrical output of 100KVA/80KW at 0.8
power factor, 415Volts at the alternator terminal. The
Engine is fitted with the following standard accessories 01.
A1 class Mechanical 02. Battery Charging Alternator 03.
Engine safety Sensors (LLOP & HWT) 04. Air Cleaner 05.
Lube Oil Filter 06. Fuel Filler
B. ALTERNATOR: 100KVA/80.00KW, alternator, 3
phase, 415V, 0.8 PF, type, screen protected, revolving
field, self-excited, self-regulated through an AVR.
Permissible overload of 10% for one hour in 12 hours of
operation.
C. CONTROL PANEL: The Control Panel is
manufactured with 16/18 gauge CRCA sheet and is
powder coated for a weather-proof and long lasting finish.
The Control Panel consists of Microprocessor based
SEDMEC electronic controller, MCCB of suitable rating,
Key Switch, Push Button (starter), LED: Battery Charging
alternator fail indication, Current Transformer, Instrument
fuses. The microprocessor electronic controller displays
the following parameters: Voltage, Frequency, Current,
Rpm, Cumulative Run Hours, Oil Pressure, Coolant
Temperature & Charging Current (DC Amp Meter). The
microprocessor electronic controller provides the
following safety to the engine: Under & Over Voltage,
Under & over Frequency, Under & Over Speed, Overload,
Low Lube Oil Pressure, and High Coolant Temperature &
Emergency Off.
D. ESSENTIAL ACCESSORIES: Fuel tank: Sub Base
Fuel tank (DRAW IN TYPE) of MS sheet of adequate
capacity.• Base Rail: Base Rail of adequate size is
provided.• Batteries: For electrical control circuit 2 no, 24
Volts, Suitable sized battery for electrical starting of DG
set
21. Dissecting kits and DR Instruments Anatomy Dissecting kit. 12 No.
Inoculation
Instruments
22. Refrigerator Vertical • Capacity: 165 liters 2 Nos.
(40C) • For220/230 V, 50/60 Hz, Operation
• Temperature Range: 0-100C
• Door: Yes
• Wheel: Yes
• No. of Shelves/Baskets: 4
• Door: 1
• Dimension: 31” H x 26” D x 74” H
• ISO Certified product with built-in fan
• CFC Free refrigerant, fan cooled condenser,
internalfluorescent light.
• Audio defrosting facility
• CFC-Free with external thermometer
• Interrelated lock, Castors at back of cabinet and
adjustable feet at front
 • Puff insulation with cyclopentane.
23. Micro Wave Oven Make: Samsung/IFB or Equivalent 1 No.
Capacity 30 Ltr.

24. Hot Air Oven These mechanical convection ovens are perfect for heating 1 Nos.
(Capacity 100Ltr.) and drying applications in research or industrial
environments. Featuring the Memmert control cock pit for
quick and intuitive control with simple touch, turn and go
operation. Durable construction with a scratch-resistant
Structured stainless steel exterior and easy-to-clean
corrosion-resistant stainless steel interior. Recessed grips
make oven easy to carry and handle and stainless steel door
can be changed from left to right side opening.
25. Rotary Shaker Designed for continues shaking of solutions in Erlenmeyer 2 Nos.
Flasks from 50ml-1000ml capacity. It is a compact beach
type table top model with platform fitted with rubber discs
mounted on ball bearing crank shafts to give an orbital
motion to the flasks, within a diameter of approximately
2".
The motor is coupled by means of a V-belt with pitch
pulley, and is mounted on a heavy and sturdy angle iron
frame S.S top. Speed is controlled by mechanical
Speed controller. To work on 220/230 Volt A.C. supply.
PLATFORM SIZE- 65 X65CM 36 FLASK OF 250ML,
Motor capacity- 0.50 HP
26. Lab Clothes Lab cloth make Hi-media 20 Nos.
27. Media Storage Rack Media storage Racks Body made by Mild steel outer body 10 Nos.
powder coating front glass to view
28. Micro-pipettes Make: Thermo Fisher Scientific 4 Nos.
different size Model: Fin-pipettes each
1-10µl -4 Nos.
20-200 µl -4 Nos.
100-1000 µl -4 Nos.
1-5 ml -4Nos.
29. Label Gun Two Row, eight space, all alpha numerical 4 Nos.
30. Electric ON/OFF Make –Sun flame or Equivalent 10 Nos.
LPG Gas Burner
31. Sealing Machine Sealing Machine Foot operated 1 No.
32. Cutting Equipments Cutting Equipment for tissue culture lab 25 Nos.
33. Shaker with 8 Ltr. Features: § Energy efficient § Durable & Low 2 Nos.
Capacity maintenance, along with following specifications: §
Scientifically designed for mixing, diffusion, VDRL test &
development of cultures. § Heavy base & compact design
for continuous use. § Heavy Removable/ Interchangeable
S. Steel platform with Lotus clamp to hold various capacity
conical flasks. § Digital control with RPM Indicator. §
Power 230V, AC, 50Hz, Mains.
34. HVAC System Split type Air Conditioner 2.0 ton capacity 4-5 star, 11 Nos
standard make
35. Biotech Heater For heating in winter 2.1 kw Biotech heater 12 Nos
36. Fogger Machine Hand Fogger machine fully Stainless Steel 1 No.
 37. Allium Ladder Aluminum Ladder 2 Nos.
38. Lux meter Digital Lux Meter 1 No.
39. Humidifier machine S.S. Body, Capacity 10 Litres 5 No.
Based on the rotating disc-splash plate principal. It
produces only super fine particels of micron size, which
become part of the atmosphere, Due to the upward thrust
imparted to the micron size water droplets, by high speed
motorized unit, they remain suspended in the atmosphere
for longer duration of time. The unit body is made up of
S.S. To work on 220/230 volts A.C. Supply.
40. Grading & Packing Complete table is made on Stainless 1 No.
Table Steel 304 body. size 3ft x 4 ft.
41. Exhaust and ceiling Electrical Exhaust and ceiling fan 5 Nos.
fans
42. LPG Cylinder Make: Bharat/Indene/HP 2 Nos.
LPC gas Cylinder with filled LPG gas
43. Servo Stabilizer Stabilize electricity as required capacity 1No.

ANNEXURE-1
PRE ENGINEERIARED STRUCTURE
Proposes to furnish a pre-engineered steel building (s) as described below. The building will consist of all
components required to construct the primary and secondary framing (wind bents, wall & roof purlins) plus
the wall and roof cladding. Hardware’s of the building are included.
Interior partition walls, electrical, plumbing, cooling, heating, foundation, masonry walls, concrete floor or
materials are also included.

GENERAL FEATURES:-
Type: TCCS (tapered column clear span)
Width: 15.85 m o/o of steel column or as per land available
Length: 24.00 m o/o of steel column or as per land available
Eave height: 4.88 m clear under the knee.
Width module: 1@15.85 m
Roof slope: 1:10
Bay spacing: 5@7.62 m
Front end frame: rigid & bearing
Rear end frame rigid & bearing
Type of bracing on roof & wall: diagonal rod bracing/angle bracing
Roof panel: 0.45mm bare galvalume profiled sheets.
Wall panel: 0.50mm colour coated galvalume sheet above brick wall.
Girts: side wall girt & end wall girt are mounted on the exterior face of the column.
Fasteners: mechanically galvanised hex- head self drilling fasteners with integral epdm seals.
Accessories: ridge cap, eave gutter & down spouts matching to roof colour.
Paint finish: all primary and secondary members shall be supplied with factory applied one coat of zinc
chromate red oxide primer and 2 coats of synthetic enamel paint on structure applied at site.
Building: condition enclosed

DESIGN LOADS (LIVE / WIND):-

Live load: 0.57 kn/m
Wind speed: 47 m/sec (as per is 875 part iii) 1987
Seismic zone [zone (z)]: iv (0.24) (as per is 1893) 2002
DEFLECTIONS:-
Vertical: l/180
Lateral: eh/100
Purlins l/180

INCLUSIONS:-
• Interior, partitions with puff panel 60mm thick 32kg density, doors and fixtures.
• Roof fans, pipe flashing, curbs.
• Wall vents, fans, doors, windows and louvers.
• Civil, Mechanical and Electrical design and materials.
• Concrete embedded plates or weld plates of any kind.
• Foundation and masonry design/ work/ materials.
• Setting of Anchor Bolt – (Physical setting or fixing). ASSPL will only check dimensional accuracy
of anchor bolt set out.
• Grouting – Grouting under column base plates with high strength grout. Electricity & Water
required during work at site.

ANNEXURE-2A
1. Hi-Tech Greenhouse

S. N. Head Description Qty

1. Hi-Tech Poly Primary hardening 1 No.
House
Total Area 1500 Sq.M
Length/ Width As Per site requirement
Ridge Height 6.0 m
Gutter Height 4.0 m
bay Size 4.0 x 9.6m
2. Entrance: Double doors with polycarbonate sheets, second door should be sliding
and made of aluminum section (3 m x 2.10 m x 2.40 m) with provision
for disinfection pit/ foot bath.
3. Structure ISI marked G.I pipe structure 2mm thick, & nut bolts etc all pre-
galvanized (hot dipped). GI Pipe of Size 96x48mm, 60mm, 48mm,
42mm and 32mm OD or 48 x 48mm, 38 x 38mm, 32 x 32mm.
4. Covering U. V. stabilized Polyethylene film 200 micron thickness, Anti drip/ fog,
Diffused (Multilayer) for covering. - Five-layers polyethylene film
(Israel Technology)
5. Internal Inside 50% white shade net, with manual gear system and shade Net
Screen Net outside, UV stabilized 50% Fixed.
6. Air curtain 3’ wide installed above door with auto ON/OFF switch attached with 3 Nos
door - One
7. Cooling By providing following system by which temperature can be lowers by
System 10° ± 2°C from outside when outside maximum humidity 62% of low
and temperature 35°c or above
8. Cooling pad Pad Area: 52.0m L x 1.5m H× 100mm thick. 7090/50
complete Framing material of Aluminum required supporting distribution &
returning piping. Plastic Profiles: Water Distribution tray Gutters, down
spout end caps & drip pan, plumbing kit, Pump 220 volt single phase 50
 cycles, Drilled PVC piping cap, pad retainer, all suspension hardware,
At velocities of 1 to 3 M/s to give efficiency from 60 to 95% Filtration:
Mesh SS filter for 30 m efficiency.
Miscellaneous: Fasteners, Galvanized, Rivet- Aluminum. Pump: Mono
block as per requirements
Fan: Axial flow belt driven net protected
Sweep Size: 48”
Blade: 6 nos. SS
Motor: 1.5HP
Qty: 10 Nos
Louvers : auto open able
9. Fogging To increase the humidity up-to 70% ± 5% by providing following
System system,
Nozzles : Micro fogging hanging type,
Lateral pipe : 16 mm LDPE,
Water Particle : 80-100 μ
Filter : online Disc
Pipe : 40/63mm PVC 6 kg/cm²
Pump : 3.0 HP
Nozzles spacing : 2.5 x 2.5m,
Discharge : 28-30 lph at 4bar pressure
Pipe & fitting : All necessary PVC pipes 40-63mm, valve and fixtures
as required
10. Green House Control Panel: Microprocessor based controller which includes
Environmental temperature, humidity and photoperiodic monitoring & controlling
Monitoring system. Temperature: ±2ºC, Humidity: ±5%, Time: ±5sec/day.
Unit Operation system: Programmable/ user selectable
Temperature: Cooling & Heating to set
Humidity: Humidifier / Fogging to set
Light: RTC Based ON/OFF
Mounted in CRC powder coated panel fitted with contactor, relay, MCB
and preventers. All power supply used for this system.
Sensor: Temperature, Humidity 4-20mA
Temperature Range: 0-100°C, ± 1%
Humidity Range: 0-100 %RH,
±1.8%
11. Heating Biotech Heat Convector electric heating system 2.1 KW completes
System circulation system to give uniform heat so that the heat can be blown
in the complete area to increase temperature. (15 nos.)
12. Electric Fitting Complete electrical wiring of green house with copper wire ISI marked,
MCB/ main switch, light/ power points for pump/ fans as per
requirement. Electricity outlets (3/1 phase) as per requirement.
13. Foundation Telescopic below the ground level with block size 3’ x 1.25’ x 1.25’ (in
cement concrete 1:2:4 mix of cement, sand and bajri gravel.
Low Tunnels Tunnel covered with polythene sheet as per requirement in whole green
house.
14. Floor The floor should be cemented and not natural soil with cover.
15. Bricks wall With 1st class in 1:6 CM, with PCC: in foundation 1:5:10, all side of
the Green House 60cm above GL & 30cm below earth surface with
plaster in 1:4 CM (1cement, 4 fine sand). Painting with 2 or more coat
of cement paint of approved colour/shade
16. Bench GI Bench top covered with GI welded mesh. Qty as pre required
 ANNEXURE-2B
1. Secondary Hardening Chamber/ Shed Net House:

S.N. Head Description Qty.

1. Shade Net Secondary hardening Unit 1 No.
House
Total Area 4000 m2
Length/ Width As per Site
Ridge Height Flat roof
Gutter Height 04.0 m
Span/ bay 5x4m
2. Entry Room Double door type ante room size 3m x 2m with proper locking system
door covered with 6mm polycarbonate sheet covering.
3. Structure G.I pipe structure 2mm thick, & nut bolts etc all pre-galvanized (hot
dipped). GI Pipes: 60mm, 48mm, 42mm & 32mm± 1mm or 48 x 48, 38
x 38 x 32 x 32mm.
4. Net Net would be UV stabilized, HDPE, and green colour 50% shade net,
90 GSM
5. Fixing fixing of net to the structure with GI profile & spring insert or pasting
wherever required
6. Civil work foundation telescopic below the ground level with block size 2.5’ x 1.0'
x 1.0'
(75x 30 cm X 30cm) All civil work will be done in ratio 1:2:4 cement,
sand, bajri gravel
7. Fogging & Drip To increase the humidity up-to 70% ± 5% by providing following
Irrigation system,
System Nozzles : Micro fogging hanging type,
Lateral pipe : 16 mm LDPE,
Water Particle : 80-100 μ
Filter : online Disc
Pipe : 40/63mm PVC 6 kg/cm²
Pump : 1.0 HP
Nozzles spacing : 2.5 x 2.5m,
Discharge : 28-30 lph at 4bar pressure
Drip Irrigation:
Drip Lateral : 16 mm LLDPE s class 2,
Discharge : 2/ 4 lph
Spacing : 0.60m x 0.30 m (L/L x N/N/ as per requirements)
8. Pipe & fitting All necessary PVC pipes 40-63mm, valve and fixtures as required and
Installation of watering system with Pump (ISI make)
9. Path/Floor Natural Soil
 Detailed BOQ

1 Land Development
S. Particulars Description Qty Unit
No
1 Land Development Land leveling, landscaping 1 Set
2 Boring 2 HP submersible 1 Set
3 Main Gate & Guard Room 15' wide and 5' height & one small 1 set
gate with 18'' brick column support
and one guard room, Board
4 Boundary wall 6' Height Chain link Fencing with 1.5' 1 Set
brick wall base above from ground
level. 200 M approx.
2 Pre Engineered Building
1 Building PEB Building as per approved 370 Sqm
drawing. Cosisting of Storage Room
for Chemicals, Washing and Media
preparation room, Sterilization room,
Inoculation room, Culture room, wash
room, passage , door etc

2 Water Tank PVC Water tank with cc Platefarm 1 Set

20000 Ltr
3 Equipment
S. Particulars Description Qty Unit
No
1 Growth Room Racks complete with LED tube light fitting, 18 Set
timer and correct design for keeping
the material
2 Auto Clave-Horizontal Type 1200 ltr, Horizontal Type, outer and lid 1 Set
made of stainless steel, radial type
closing arrangement
3 Auto Clave-vertical Auto Clave vertical, double walled 1 Set
450 X 600 mm
4 Air Shower Airshower to remove all dust and a 1 Set
barrier for entry of insect
5 Laminar Air Flow –Double Horizontal Laminar Air flow bench of 9 Set
ftx 6ftx-2ftx2ft. Stainless Steel Top,
Gas Cock No.1 Glass/Acrylic side
panel.

6 Air Conditioner Air conditioner 2 ton 5 Star quality 11 Set

7 Plant Stacking Trollies for keeping the plants for cutting 6 Set
8 Positive Pressure Unit To induct clean air inside the lab 4 Set
9 Automatic Temperature Recorder Centerlized PLC & HMI Automation 1 Set
system to record & control temp,
humidity & light in the greenhouses &
Growth room

10 Media Preparation Equipment Stainless Steel vessel 25 ltr 1 Set

11 Glass Bead Sterlizer tissue culture use 9 Set
12 Water Purifier 1000 LPH 1 Set
13 De Humidifier 3 Set
14 Ultrasonic humidyfier 10l Capacity 5 Set
15 Electronic Balance 1 mg, and 0.1 mg 2,1 Set
16 Bottle Washing Machine 1 Set
17 Media Trolly for media filling 1 Set
 18 Heater Air heater 12 Set
19 Tray For Bottle Moment 600 Set
20 Air Curtain 3 ft. wide outer cover made of MS duly 3 Set
painted
21 Electronic Balance 500gm, Indian make 1 Set
22 pH Meter cum conductivity meter 5 No.
(Digital)
23 Refrigerator Vertical (4°C) 165 ltr. Min. Standard make 2 Set
24 U V Light System complete fitting 1 Set
25 Label Gun two row, eight space, all alpha 4 Set
numerical
26 Fumigation Machine 1 Set
27 Growth Room Tray trays needed for keeping the bottles in 1,250 Set
rack
28 Sealing Machine 1 Set
29 Hygro Thermometer digital 5 Set
30 Water Cooler 2 Set
31 Magnetic Stirrer 2 ltr capacity 2 Set
32 Mosquito Trap 2 Set
33 Attendance Machine 1 Set
34 Lux Meter 1 Set
35 EC Meter 1 Set
36 Hot Plate 2 Set
37 Peristaltic pump for media 2 No.
dispensing
38 Computer desktop 2 Set
39 Printer (4 In One) desk & laser 2 Set
40 Stereo Zoom Microscopes with 1 Set
Camera
41 Vertical Distillation Apparatus 5 Liter Capacity 1 Set
42 Dissecting Kits and Innoculation 12 No.
Instruments
43 Micro Wave Oven Capacity 30 Ltr, Make Samsung/IFB 1 No.
or Equivalent
44 Hot Air Oven Capacity 100 Ltr 1 No.
45 Media Storage rack 10 No.
46 Cutting Equipments Cutting Equipments for TC Lab 25 No.
47 Rotary Shaker 2 No.
48 Fogging Machines Hand Fogger Machines fully stainless 1 No.
stell
49 Shaker Shaker with 8 ltr capacity 2 No.
50 Aluminum Ladder 1 No.
52 Photo Periodic Morphogen 4 No.
controller
51 Misc Equipment 1 Set
4 Transformer, Electrical & DG Set
S.No Item Description Qty Unit
1 Electric Wiring Supply and Laying of electrical 1 Set
Aluminium armored cable of
rerequired capacity, complete electric
wiring Control panel, LT panel etc
complete in all respect

2 DG Set with shed 110 KVA DG (110 KVA) with shed & CC 1 Set
Platfarm, earthing etc complete in all
respect.
 5 Furniture & Fixture
S.No Item Description Qty Unit
1 Executive Chair furniture 10 Set
2 Computer Chair furniture 5 Set
3 Computer Table furniture 5 Set
4 Laminar Chair furniture 24 Set
5 Visitor Chair furniture 20 Set
6 Office Table furniture 3 Set
7 Shoe Rack furniture 4 Set
8 Writing Board furniture 4 Set
9 Conference Table Set furniture 1 Set
10 Cupboard furniture 2 Set
11 Misc Stationary& Other Item As required 1 Set

6 Chemicals
S.No Item Description Qty Unit
1 Blade Handle Stainless Steel Made 200 Set
2 Chemicals Various Chemicals Used in Tissue 1 Set
Culture Lab (Lumpsum)
3 Agar Agar 100 Set
4 ISO Alcohol 400 Set
5 Forceps Stainless Steel Made 100 Set
6 Cling Film Film To Wrap The Bottles 250 Set
7 Lab Coat 40 Set
8 Label Roll 150 Set
9 Gas Burner 1 Set
10 MIS Formats Misc MIS Complete Set To Cover All 1 Set
Activities
11 Fungicide 5 Kg 10 Set
12 Insecticide 5 Kg 10 Set
13 Steel Trays 6" 12 Set
14 Forcep And BP Handle Stand 40 Set
15 Instruction Posters 4 Set
16 Misc Posters 5 Set
17 Cotton 200 Set
18 Aluminum Foil 50 Set
19 P P Bags In Kg 60 Set
20 Surgical Blade 5,000 Set
21 Face Cap Lab Wear Reusable (Made of Cloth) 20 Set
22 Face Mask Lab Wear Reusable (Made of Cloth) 20 Set
23 Regent Bottles 20 Set
24 Liquid Soap 500 Set
25 Slippers 20 Set
26 Cylinder 5 Set
27 Brown Tap 100 Set
28 Formaldehyde 35 Ltr 200 Set
29 Spray Bottle 10 Set
30 Apron 20 Set
31 Wall Clock 5 Set
32 Name Plate 5 Set
33 Wiper 5 Set
34 Dustbin Different Sizes 10 Set
35 Duster 15 Set
36 Cutting Paper Lump Sum 1 Set
37 Mop 20 Set
38 Funnel 1 Set
39 CCTV Camera Complete set with monitor 16 Set
40 Plug tray 5,000 No.
41 Misc. Items As required 1 Set
7 Glassware, Metalware, Bottles, Lids and Labware
S. Item Description Qty Unit
No
1 Test tube glassware 200 Set
2 Test tube stand glassware 50 Set
3 Petri dish glassware 70 Set
4 Beaker 100 ml glassware 6 Set
5 Conical flask 100 ml glassware 6 Set
6 Beaker 250 ml glassware 6 Set
7 Conical Flask 250 ml glassware 6 Set
8 Measuring cylinder 10 ml glassware 6 Set
9 Beaker 500 ml glassware 3 Set
10 Pipeettes 1 ml glassware 3 Set
11 Pipettes 10 ml glassware 3 Set
12 Pipettes 2 ml glassware 3 Set
13 Pipettes 5 ml glassware 3 Set
14 Pippette Stand glassware 1 Set
15 Glass Bottles with Lids 80,000 Set
16 Multipurpose Utility Trays Plastic, Perforated Trays 50 No.
17 Multipurpose Utility Trays Plastic Trays 50 No.
18 Laboratory Spatula, 6'' S S Stainless Steel, 6'' Spatula, Pack of 6 6 Sets
19 Long forcep Size 12'', stainless steel, pack of 6 6 Sets
20 Scalpel Holder Stainless Steel 20 No.
21 Spirit Lamp Stainless Steel 12 No.
22 Scissor Stainless Steel, 6 '' 12 No.
23 Scalpel blades Stainless Steel, No. 11, Pack of 30 2 Set
24 Beaker Tongs Stainless Steel with plastic grip 3 No.
25 Micro pippettes 1-10 micro ltr, 20-200 micro ltr, 100- 4 No.
1000 micro ltr, 1-5 ml Each
26 Misc. Items As required 1 Set
8 Hardening Chamber
S.No Item Description Qty Unit
1 Primary Hardening Chamber Supply and Installation of Hi-Tech 1 Set
Poly House 1500 Sqm., as per
technical specification.
2 Secondary Hardening Chamber Supply and Installation of Shade Net 1 Set
House 4000 Sqm. Flat Roof, as per
technical specification.

9 Protocols, Mother Culture & Packaging Material

S.No Item Description Qty Unit
1 Protocols Detailed protocol for 5 crops shall be 5 Set
provided.
2 Mother Culture 3000 Mother of Banana, Strawberry, 3000 Nos
Bamboo & Pineapple
3 Packaging Material Corrugated Cardboard 5 Ply Strong 1,200 Nos
box & Thermocol Box.
4 Certification and accreditation to the As required -
tissue culture Lab