USER

MANUAL

K-MINC-1000
Benchtop Incubator

Please familiarise yourself with

the safety instructions
before using the device.
Accessories and devices may
be used only by physicians and
medical assistants with the
appropriate technical qualification.
0123
K-MINC-1000 INSTRUCTIONS FOR USE

GENERAL INFORMATION
COPYRIGHT
WARNING: This manual contains information that is subject to copyright. All rights
READ THIS MANUAL! reserved. This manual should not be photocopied, duplicated on
Please familiarise yourself microfilm or otherwise copied or distributed, completely or in part,
with the contents of the without the approval of WILLIAM A. COOK AUSTRALIA PTY LTD.
manual before using the
device. Failure to comply Some of the parts and equipment referred to in this manual bear
with these instructions registered trademarks but are not identified as such. It should therefore
may result in damage to not be assumed that the absence of the trademark indicates that any
device, device contents, given designation is not subject to trademark protection.
and/or patient or user Users of WILLIAM A. COOK AUSTRALIA PTY LTD products should not
injury. This device should hesitate to contact us if there are any unclear points or ambiguities in
only be used by qualified this manual.
personnel.

© WILLIAM A. COOK AUSTRALIA PTY LTD 2006

WARNING:
Document No: MNM30016
ELECTRIC SHOCK
HAZARD! VERSION 11, October 2006
The equipment is to be
used only with electrical
systems complying with Service address:
all IEC, CEC and NEC Please refer to your local Cook distributor for details of your nearest
requirements! authorised service agent.

CAUTION:
Any adjustment,
modification or repairs to
the equipment should be
carried out by persons
authorised to perform
them.

WARNING - CONFIDENTIAL PROPRIETARY PROPERTY

This document is owned by WILLIAM A. COOK AUSTRALIA PTY. LTD. It contains confidential proprietary trade secret
information and must not be copied. The document and the information it contains can be used only by the recipient for
the specific use for which it was requested. All other use is strictly prohibited. This document must be returned to
WILLIAM A. COOK AUSTRALIA PTY. LTD. immediately upon request by WILLIAM A. COOK AUSTRALIA PTY. LTD.
By possession of this document, the possessor expressly agrees to comply with these terms.

MNM30016-V11
K-MINC-1000 INSTRUCTIONS FOR USE

TABLE OF CONTENTS
EXPLANATION OF PICTOGRAMS iii
HOW TO USE THIS MANUAL iv
Warnings, Cautions, and Important Notes iv
Manual Structure iv
1. SAFETY INSTRUCTIONS 1
2. ABOUT THE K-MINC-1000 2
2.1 Intended Use 2
2.2 Contra-indication for Device Use 2
3. INSTALLATION AND SET-UP 3
3.1 Unpacking 3
3.2 You Need to Supply 4
3.3 Front of the Device 5
3.4 Rear of the Device 6
3.5 Supply Voltage Selection 7
3.6 Device Placement 8
3.7 Gas Supply 8
3.7.1 Gas Mixture Required 8
3.7.2 Gas Bottle Regulator Recommendations 9
3.7.3 Connection to Gas Bottle 10
3.7.4 Series Connections of Devices 11
3.7.5 Other Gas Connections 11
3.8 Humidification Flask 12
3.9 Activating the Device 13
3.10 Chamber Temperature Selection 13
3.11 Gas Flow Rate Selection 14
3.12 Insertion of the Culture Dishes 14
3.13 First Time Use 14
3.14 Alarm Conditions 15
3.14.1 No Gas Flow or Gas Flow Out of Range 15
3.14.2 Temperature Out of Range 15
3.14.3 External Alarm 15
3.14.4 Low Battery (power-fail external alarm) 15
4. SERVICE AND MAINTENANCE 16
4.1 Changing Internal Filter, Gas Lines & Humidification Flask 16
4.2 Cleaning the Device 16
4.3 Biannual Functionality Testing 18
4.4 Inspection by an Authorised Service Technician 19
4.5 Fuse Replacement 20
4.6 Return Procedure 21
5. ACCESSORIES 22
6. TECHNICAL DATA 23
7. TROUBLESHOOTING 24
8. LIMITED WARRANTY 26
8.1 Liability 26
9. INDEX 27
10. INSTALLATION AND SET-UP CHECKLIST 29

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K-MINC-1000 INSTRUCTIONS FOR USE

MNM30016-V11 ii
K-MINC-1000 INSTRUCTIONS FOR USE

EXPLANATION OF PICTOGRAMS
The following pictograms appear on the device

Before connection, read the manual!

Standby/On

Start/Stop

Outlet for energy/signals

Inlet for energy/signals

On

Off

Symbol for type B equipment

Gas Flow Meter

IP31
Degree of enclosure protection from solid
object and liquids

Heater

Temperature

Contacts

Gas Flow Status

Up/down buttons

Cylinder connection

Alarm

Communication port

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K-MINC-1000 INSTRUCTIONS FOR USE

HOW TO USE THIS MANUAL

Warnings, Cautions, and Important Notes
Throughout this Instructions for Use, blocks of text may be accompanied
by a pictogram and/or printed in bold type. These blocks are WARNINGS,
CAUTIONS, and IMPORTANT NOTES and they are used as follows:
WARNING! The safety of the incubator contents may be
involved. Disregarding this information could result in
injury to the operator, device or the contents!

WARNING! Biological hazard

WARNING! Electric shock hazard

WARNING! Explosion hazard

CAUTION! These instructions point out special service

procedures or precautions that you must follow to avoid
damaging the device!

IMPORTANT NOTE! This provides special information that

facilitates maintenance or clarifies important instructions.
Please pay particular attention to the sections Safety
Instructions and Purpose of the Device.

Manual Structure

This manual has a table of contents (page i) to help you find section titles
quickly. These titles may then be easily located on the relevant page by
looking for title blocks.

Additionally, an index may be found on page 27.

There is a list of error messages and warning indications found on page

24 to help you to troubleshoot problems.

MNM30016-V11 iv
K-MINC-1000 INSTRUCTIONS FOR USE

1. SAFETY INSTRUCTIONS
These instructions for use describe the operation of the device and the
WARNING: Please accessories.
familiarise yourself with
the safety instructions It is essential to use this document to familiarise yourself with the
before using the device! functions and the operation of the device before use.
Not following these instructions can result in damage or breakdown
of the device and accessories.
WARNING: Electric
shock hazard!

Internal circuitry is energised whenever the incubator is connected to

mains power, and the mains power switch on the back of the incubator
is turned on, irrespective of whether or not the power STANDBY touch-
pad switch on the front panel has been pressed. Always turn off the mains
power switch on the back panel of the incubator and disconnect the unit
from mains power before fuse replacement, cord replacement, or
cleaning. Should any power cord or plug associated with the incubator
become cracked, frayed, broken or damaged it must be
replaced immediately.

To reduce the risk of electric shock, do not remove covers. Refer servicing
to qualified service personnel.

Protect the device from being splashed by liquid. Keep the power switch
dry. Should any liquid enter the device, discontinue use immediately.

WARNING: No user Please refer all servicing to an authorised service agent.

serviceable parts inside!

WARNING: This
device should only be
operated by appropriately
qualified personnel !

WARNING: Device Do not use in an area where flammable gases are present.
can cause explosion in
presence of flammable
gases! Use only the Braided PTFE lined gas connecting hose supplied with the
K-MINC-1000 to connect it to a gas supply. The use of another tubing
may result in the loss of desired gas concentrations.
WARNING: Use the
correct gas connecting
hose! For your own safety use only original accessories (see §5).

WARNING: Use
only original accessories!

MNM30016-V11 1
K-MINC-1000 INSTRUCTIONS FOR USE

2. ABOUT THE K-MINC-1000

2.1 Intended Use
The K-MINC-1000 is a microprocessor-controlled humidified, gassed
incubator intended for use in cell culture. The instrument is designed to
maintain temperature accurately at a user specified temperature within
the range of 350 to 400 Celsius, and to maintain accurate gas flow at a
user specified flow rate within the range of 15ml/min to 25ml/min. The
incubator utilises premixed gas to maintain optimum culture conditions
within the incubation chambers. All components in contact with the gas
flow, including external and internal gas lines, have undergone rigorous
testing to ensure a toxin free environment.

2.2 Contra-indication for Device Use

Should any electrical or mechanical fault occur while using the
K-MINC-1000, stop using the device until it has been checked by
authorised service personnel.

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K-MINC-1000 INSTRUCTIONS FOR USE

3. INSTALLATION AND SET-UP

An installation and set-up checklist has been included in the rear of
To ensure no the manual as §10. This may be used to help ensure that all steps
interruption to the are followed.
functioning of the
K-MINC-1000, we
strongly recommend
the use of:
3.1 Unpacking
1. An automatic gas
cylinder changeover Items supplied
unit that can Please find the following items supplied:-
maintain a
minimum pressure
of 135kPa.
2. An uninterruptible 1 2
power supply (UPS).
3. A remote alarm to
alert staff to any
failures in the gas or
power supply.

It is important to 3
retain package for future
use. (Refer to §4.6 -
Return Procedure)

4 5

1. K-MINC-1000 User Manual

2. K-MINC-1000 Unit
3. K-MINC-CTS-S Disposable Humidification Flask
4. Power Cord
5. K-MINC-BCT-10-300 Braided connecting hose for
K-MINC-1000 (3 metre length)

Check the device and all accessories immediately upon receipt to make
sure the contents are complete and that nothing is damaged. The
manufacturer will only honour claims for compensation, which are
forwarded immediately to the sales representative or the authorised
service company.
Remove all items from plastic covers except item 3 (humidification flask)
which needs to be handled under sterile conditions (refer to §3.8)

MNM30016-V11 3
K-MINC-1000 INSTRUCTIONS FOR USE

3.2 You Need to Supply

Items required that
are not supplied are listed Items not supplied:-
below.
l Premixed medical grade gas cylinders. All gas concentrations
should be within ±0.2% and cylinders should be supplied with a
certificate of analysis, refer to §3.7.1.
l Single stage high purity gas regulator capable of supplying the
gas listed above at 150kPa (1.5 bar, 22psi), refer to §3.7.2.
l A source of sterile distilled water.
l A pressure manometer (to measure the K-MINC-1000 input gas
supply). This item is not required if the regulator has gas output
display gauges.
l A 14 mm (9/16") spanner.
l If a longer connecting hose is required, refer to §5.

MNM30016-V11 4
K-MINC-1000 INSTRUCTIONS FOR USE

3.3 Front of the Device

10
14
4 6

1 2 3 5 7 8 9 11 12 13

1. Power On/Standby Touch-pad

2. Mains Power Indicator
3. Temperature Display (Left Chamber)
4. Heating Status Indicator (Left Chamber)
5. Temperature Selection Touch-pads (Left
Chamber)
6. Heating Start/Stop Touch-pad (Left Chamber)
7. Gas Flow Rate Display
8. Gas Flow Status Indicator
9. Gas Flow Rate Selection Touch-pad
10. Gas Flow Start/Stop Touch Pad
11. Temperature Display (Right Chamber)
12. Heating Status Indicator (Right Chamber)
13. Temperature Selection Touch-pads (Right
Chamber)
14. Heating Start/Stop Touch-pad (Right Chamber)

MNM30016-V11 5
K-MINC-1000 INSTRUCTIONS FOR USE

3.4 Rear of the Device

1 2 3 4 5 6 7

1. Main Power Switch

2. Power Entry Module
3. Gas Inlet
4. Gas Outlet (blocking plug shown)
5. Gas Vent Holes
6. External Alarm Contacts
7. COOK-Net Connectors (used for calibration
and servicing)

MNM30016-V11 6
K-MINC-1000 INSTRUCTIONS FOR USE

3.5 Supply Voltage Selection

WARNING:
ELECTRIC SHOCK
The K-MINC-1000 may be configured to operate on one of the voltages
HAZARD!
listed below. Check that the voltage selector on the power entry module
Determine if the available is set to the correct mains voltage for your region. If the voltage is
voltage corresponds to changed, it may be necessary to replace the fuses to maintain
your device. Connecting electrical safety.
to the wrong voltage will
cause the device to
malfunction or may
permanently damage
the device!

Fuses must only be

replaced with those of
appropriate rating and
type. Both fuses must be
of the same rating and
type!

To alter the voltage selection:

The power supply cable 1. Ensure that the power cord is disconnected from
must be equipped with a the power entry module.
safety plug. Use the
enclosed power cable for 2. Open the door of the module (hinge is on the
the connection between lower edge). A small screwdriver may be used.
the power plug and the 3. Remove the selector wheel and reinsert it so that
device socket! the desired voltage indicator is facing directly
outwards, such that it will be showing through
the door window.
If replacement mains
cord within the USA and 4. Using the table below, ensure that the
Canada is required use appropriate fuses are fitted. Refer to §4.5 for
only 18AWG 3conductor fuse replacement.
suitable cord listed as 5. Close the door of the module.
“Medical grade”.
6. Connect the power cord to the power entry
Grounding will only be module and then the supply point.
reliable if the equipment
is connected to a
corresponding hospital Supply Voltage Suitable Voltage Fuse Required
grade socket! (Vac) Selector Setting (V)

100 100 T2AL250V

115 120 T2AL250V
120 120 T2AL250V
220 220 T1AL250V
230 240 T1AL250V
240 240 T1AL250V

MNM30016-V11 7
K-MINC-1000 INSTRUCTIONS FOR USE

3.6 Device Placement

WARNING:
EXPLOSION HAZARD!
The K-MINC-1000 should be placed on a level secure surface, away from
Do not use the device in
heaters, coolers, air-conditioning outlets, mists, splashes and exposure to
the presence of
direct sunlight. It must not be placed in the presence of flammable gases.
flammable gases!

WARNING:
ELECTRIC SHOCK
HAZARD!
Do not immerse the
incubator!

The ambient
temperature should be
between 20 and 30°C for
the K-MINC-1000 set-
point of 35 to 40°C. For
set-point of 37°C,
ambient operational
range is extended to 18-
32°C . All temperature
set-points are maintained 3.7 Gas Supply
within ± 0.2°C accuracy.
Relative humidity should 3.7.1 Gas Mixture Required
be between 30 and 90%
To maintain the correct operating pH of 7.2-7.4 in bicarbonate buffered
for the device to function
media the concentration of CO2 in the atmosphere in contact with the
correctly.
media must be strictly controlled. The concentration of CO2 (expressed
as a percentage) required to maintain the correct operating pH is
dependant on the chemical composition and concentration of the media,
WARNING: Ensure
the altitude at which the device is being operated and the humidification
that the appropriate high
status of the atmosphere in contact with the media. The correct
purity gas mixture is
percentage CO2 for the desired pH can be determined from the
selected for the altitude
graph provided.
of use and the culture
media being used!
At sea level, the Cook Culture System range of media will give an
approximate pH of 7.4 if 5.0% CO2 is used and 7.3 if 6.0% CO2 is
Using the Cook
used in the gas mixture. Cook recommends the use of 6.0% CO2 as it
Culture System at sea
promotes a more rapid recovery to an acceptable pH. Cook recommends
level a 6% CO2, 5% O2,
a reduced oxygen concentration from the normal atmospheric
and 89% N2 blend high
concentration, to a more physiological value of 5-8% in the atmosphere
purity gas mixture is
in contact with the media, as this may reduce reactive oxygen
recommended.
species formation.

If using the K-MINC-1000 at other than sea level, the following graph
may be used to find the approximate CO2 percentage to achieve a
pH of 7.3.

MNM30016-V11 8
K-MINC-1000 INSTRUCTIONS FOR USE

% CO 2
r e q u ir e d to m a in ta in p H 7 .3 v s A lti tu d e

15

14

13

12

11

10

5
0 1000 2000 3000 4000 5000 6000

m e tre s a b o v e s e a le v e l

If a different medium is used or a different pH is required, then the end

user will need to determine the appropriate gas mix for connection to
the device.
3.7.2 Gas Bottle Regulator Recommendations
WARNING: The following information is a recommended guide for the selection of
The gas inlet must be a gas bottle regulator used with the K-MINC-1000. Following these
connected to a regulated guidelines will provide a reliable gas connection between a gas cylinder
pressure source set to and the K-MINC-1000.
150kPa (Tolerance of
±15kPa) for the device The gas regulator chosen in conjunction with the K-MINC-1000 is an
to operate correctly! important part of the gas delivery system and must be designed and
manufactured to carry medical gases. As the gas specified to be used with
the K-MINC-1000 is of high purity and accuracy it is important that the
regulator used does not contaminate the gas stream.
Please supply the information below when ordering your gas regulator:
l Single stage high purity regulator.
l Metal-to Metal diaphragm seal.
l Stainless Steel diaphragm, which doesn’t contaminate high purity
gas streams.
l Dual scale gauges (optional).
l Fully configured for a special blend medical grade gas cylinder.
l Delivery pressure of 150kPa ±15kPa.
l A minimum flow capability of 360ml/min per K-MINC-1000 is
required during the purge cycle.
l The outlet fitting of the regulator is to be a 4S-1/4" SWAGELOK®
fitting to fit the connecting hose supplied with the K-MINC- 1000.

MNM30016-V11 9
K-MINC-1000 INSTRUCTIONS FOR USE

3.7.3 Connection to Gas Bottle

Do not insert any The K-MINC-1000 and connecting hose are fitted with SWAGELOK® 1/4"
form of flow restrictor or series tube fittings. A pressure regulator with these fittings should also
flow regulator in the gas be used.
supply system. A pressure These fittings use a tapered ferrule to provide a leak-free seal upon
regulator set to a connection.
nominal 150kPa is
required.

Firmly tighten the

connecting hose(s) to the
K-MINC-1000 fitting and
ensure that the blanking
plug is also firmly
tightened (if used).

The ferrules on the connecting hose have been pre-swaged before

shipping, so any ferrule and backnut on the bottle mounted regulator
will be surplus to requirements.
It will be necessary to connect the hose to the regulator outlet fitting and
also to the K-MINC-1000 inlet fitting, thus the instructions below must be
performed on each end of the connecting hose.
To connect the hose to a fitting:
1. Ensure that the fitting body, tube end and ferrules are free of any
foreign materials.
2. Insert the tube adaptor with preswaged ferrules into the fitting body
until the front ferrule seats.
3. Tighten the back nut firmly by hand. Using a 14mm (9/16") spanner
tighten the nut slightly (usually 1/8 turn or less).

Check the seal is gas tight by covering with soapy water and looking for
the presence of bubbles. If bubbles are observed, disconnect and repeat
from step 1, tightening the nut further if no foreign material is found in
the fitting.
Blanking Plug

The same reliable leak-proof seal can be obtained every time the
connection is made.
The blanking plug, shown above, must be tightened on to the unused
gas connection.

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K-MINC-1000 INSTRUCTIONS FOR USE

3.7.4 Series Connections of Devices

In situations where multiple K-MINC-1000 devices are to be operated
from a single gas supply point, sequential connection of the units
Keep your unused is possible.
blanking plugs securely
stored in the event that
independent operation of
the units is required in
the future.
Ensure that your pressure
regulator is capable of
flowing sufficient as for
your needs. If in doubt,
check the regulated
pressure is still a nominal
150 kPa with all series
connected
devices purging.

To connect units in this fashion:

1. Remove the blanking plugs from the second gas

connection point of all but the last unit in the
sequence.
2. Connect the hose of the last unit to the second
gas connection point of the previous unit.
3. Repeat step 2 until all units are linked.

The gas connecting hose can be ordered in different lengths to suit

individual installation requirements. See accessories §5.

To ensure no 3.7.5 Other Gas Connections

interruption to the
For other gas connections or existing gas delivery systems, (eg gas
functioning of the
cylinder change over units or permanently installed gas supply systems)
K-MINC-1000, we
it is recommended that the user contact the relevant gas equipment
strongly recommend
supply centre for installation advice, eg BOC Gases or Airliquide. For gas
the use of an automatic
connection information contact your SWAGELOK® distributor
gas cylinder changeover
(www.swagelok.com).
unit that can maintain a
minimum pressure
of 135kPa.

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K-MINC-1000 INSTRUCTIONS FOR USE

3.8 Humidification Flask

The K-MINC-1000 uses a disposable humidification flask, gas line and
WARNING: filter set (reorder code K-MINC-CTS-S). To prepare and install the
BIOLOGICAL HAZARD! humidification flask:-
Do not use a
contaminated
humidification flask in 1. Under laminar flow conditions and using aseptic techniques, fill
the device. It is the humidification flask with 170ml of sterile water. Ensure the
recommended that the flask cap is pushed on in the correct orientation. Failure to seal
sterile humidification the cap may result in loss of gas flow to chambers.
flask be replace regularly
with a maximum period
of use being 4 weeks.

Front of Device

Bacterial Filter

Gas Outlet Humidification Flask

2. Insert the flask into the appropriate position in the chamber.

The tubing exiting the flask should face the rear of the device.
Connect the filter with the Luer fitting to the gas outlet as shown
below. Rotate the filter and tubing anticlockwise 180° before
pressing onto the Luer fitting and rotating clockwise to lock the
filter in place. This will ensure no kinking or twisting of the
tubing occurs.

3. Insert the gas lines into the centre slots on each side of the
chamber as per the diagram below. Check to ensure that the gas
lines are not kinked and are seated correctly into the slots to
ensure they are not crimped when the lid is closed.

See detail

Centre slot with gas line inserted

Tube detail

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K-MINC-1000 INSTRUCTIONS FOR USE

3.9 Activating the Device

WARNING:
ELECTRIC SHOCK Turn on the mains power switch at the rear of the device. The incubator
HAZARD! will commence operation, using default temperature and gas flow rate
Internal circuitry is settings, without the need to press the ON/STANDBY touch-pad on the
energized whenever the front panel.
incubator is connected to
mains power, and the
mains power switch on When either lid is closed or gas flow is turned on, the chambers are
the back of the incubator automatically purged to re-establish the appropriate gaseous
is turned on, irrespective environment quickly. The automatic purge flow rate is preset and
of whether or not the operates independently of the set flow rate.
power STANDBY touch-
pad switch on the front When the incubator has just been turned on or when the set
panel has been pressed. temperature has been adjusted, the temperature alarm is
Always turn off the mains deactivated for 40 minutes to allow the incubator to reach stable
power switch on the back conditions without constantly alarming.
panel of the incubator Two power switches are needed to ensure that operation of the
and disconnect the unit device will not be interrupted by a temporary loss of mains power.
from mains power before The incubator can be placed in stand-by mode by pressing the
fuse replacement, cord power ON/STANDBY touch-pad on the front panel. The Mains
replacement, cleaning or power switch on the rear panel must be turned off to shut the
removal of the cover. incubator down totally.
Should any power cord or
plug associated with the
incubator become
cracked , frayed, broken
or damaged it must be
replaced immediately!
Determine if the available
voltage corresponds to
your device. Connecting
to the wrong voltage will
cause the device to
malfunction or may
permanently damage
the device!
3.10 Chamber Temperature Selection
When first turned on the incubator will default to a temperature of
Ensure that gas 37.00C.
supply is available to the
device. Front panel displays will show actual temperatures of each chamber in
degrees Centigrade (0C).
To alter the temperature settings depress the up or down arrow
temperature selection touch-pad on the front panel. The temperature
will scroll up or down in 0.10C increments.

When desired temperature is reached remove finger from touch-pad.

After approximately one second, the LED display will switch back to
show the actual chamber temperature and the new set temperature
will be saved.

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K-MINC-1000 INSTRUCTIONS FOR USE

3.11 Gas Flow Rate Selection

When first turned on the incubator will default to a gas flow of 15ml/min.
The front panel display will show actual gas flow in millilitres per minute
(ml/min).
To alter the gas flow rate settings depress the up or down arrow gas flow
selection touch-pad on the front panel. The gas flow will scroll up or
down in 5 ml/min increments.
When desired flow rate is reached remove finger from touch-pad. After
approximately one second the LED display will switch back to show the
actual flow rate and the new set flow rate will be saved.

3.12 Insertion of the Culture Dishes

The K-MINC-1000 has been designed to be used with NUNC® four-well
dishes, 60mm or 30mm Petri dishes.
Four-well dishes or Petri dishes may be placed on the chamber base.
Ensure that they sit securely in the grooves designed to accept them.
There should be direct contact between the base of the dish and the
surface of the chamber base.
Use the magnetic backed white board on the chamber lids to record
culture dish contents with a marking pen. Pen markings can be removed
using an alcohol solution.

3.13 First Time Use

Leave the K-MINC-1000 to operate with both chambers at 37°C and gas
flow at 15ml/min for 24 hours to ensure any residual out-gassing of
components is complete.
Test each K-MINC-1000 chamber for pH maintenance using culture media
containing phenol red indicator (15µg/ml). Adjust the gas flow to 15
ml/min and place the culture media in culture wells in both incubation
chambers. After overnight incubation, observe that the phenol red
indicator is the correct colour (salmon pink).
If the unit is being used as an ICSI workstation a flow rate of 25ml/min is
advised. If the lids are opened infrequently the flow rate can be reduced
to 15 - 20ml/min.
The K-MINC-1000 is now successfully installed and commissioned.

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K-MINC-1000 INSTRUCTIONS FOR USE

3.14 Alarm Conditions

3.14.1 No Gas Flow or Gas Flow Out of Range
The gas flow display will show a "CO2" display if gas
flow differs from the set gas flow by greater than ±25%
(including no gas flow) for longer than a nominal
10 minutes.
The gas flow display will show an "Err" display and the
external alarm will be activated if gas flow differs from the
set gas flow by greater than 4 ml/min (including no gas
flow) for longer than 15 minutes. An "Err" display will take
precedence over a "CO2" display.

3.14.2 Temperature Out of Range

When the incubator has just been turned on or when the
set temperature has been adjusted, the temperature alarm
is deactivated for 40 minutes to allow the incubator to
reach stable conditions without constantly alarming.
After this time the temperature display will show "ERR" and
the external alarm will be activated if the set temperature
differs by greater than ±0.2°C, for longer than 2 minutes.

3.14.3 External Alarm

The K-MINC-1000 has the facility to connect to an external alarm monitor
that will alert staff of activated alarms out-of-hours. If an external alarm
is connected it will be triggered six minutes after the loss of mains power.
This external alarm is normally an "open circuit" and will "close" in the
event of the above. See §6 Technical Data for alarm contact rating. If the
power is restored within the limits of the trigger time the alarm would not
be activated.
Customer requirements for the external alarm monitor should be referred
to a company that specialises in this type of equipment.
3.14.4 Low Battery (power-fail external alarm)
In order for the mains power fail ‘external alarm’ feature described in
§3.14.3 to function, a battery has been fitted in the K-MINC-1000. This
battery is a long-life, no maintenance type which should offer many years
of service.
The K-MINC-1000 has an audible low battery warning that will sound
when the battery is almost exhausted and the incubator is operating.
Should this low battery alarm sound the K-MINC-1000 will continue to
operate, however the power-fail alarm may not function correctly. The
unit must be returned for servicing, as detailed in §4.6.
The alarm sound is a 0.1 second beep every 15 seconds.

MNM30016-V11 15
K-MINC-1000 INSTRUCTIONS FOR USE

4. SERVICE AND MAINTENANCE

4.1 Changing Internal Filter, Gas Lines &
WARNING:
To guarantee safe
Humidification Flask
operation, it is necessary To preserve the device and ensure its proper functioning, proper service,
to carry out proper care maintenance and storage must be provided for. To protect the patient
and maintenance of the from infection, all accessory items that come into contact with human
device and accessories. tissue (example test tubes and tubing) must be sterile. Accessory items
Regular checks to confirm must be discarded after each patient use.
correct functioning of the
device are recommended! To change the internal filter, gas lines and humidification flask:

New and repaired 1. If the gas flow is on, turn it off by depressing the Gas Flow
products must be ON/STANDBY touch-pad.
prepared and tested 2. Lift the lid of the centre chamber and detach and remove the old
according to the manual filter, gas line and humidification flask.
instructions before you
3. Prepare and insert a new flask as detail in §3.8.
use them.
4. To recommence operation of the incubator depress the Gas Flow
ON/STANDBY touch-pad tore-start gas flow checking that
WARNING: bubbles can be observed in the humidification flask, and that the
BIOLOGICAL HAZARD! gas line supplying each chamber is not kinked or pinched. Close
Do not use a the lid of the centre chamber and adjust the flow rate if required.
contaminated
humidification flask in
5. Allow 4 hours for the water to heat and saturate
the device. It is with CO2.
recommended that the This process can be accelerated by:
sterile humidification
flask be replaced each 1. Pre-heating the new humidification flask to 35°C in step 3, and
time the sterile water 2. Purging the system at least 3 times in quick succession in step 4.
needs refilling in order to
This will allow immediate use of the K-MINC-1000.
avoid bacterial
contamination of
contents.

WARNING:
ELECTRIC SHOCK 4.2 Cleaning the Device
HAZARD!
Do not immerse the Before periodic cleaning of the device remove:
incubator! 1. The power cable from the connection at the rear of the unit.
2. The humidification bottle.
3. The contents of the incubation chambers.

Cook recommends aqueous 70% alcohol solution. If other appropriate

solution is preferred, use instructions provided by the disinfectant
manufacturer to establish the correct fluid concentration. Moisten a cloth
with the disinfectant solution and wipe the surface of the device. Do not
submerge the device in the disinfectant solution.

MNM30016-V11 16
K-MINC-1000 INSTRUCTIONS FOR USE

Clean the gas vents located towards the inside rear corner of the chamber
lids by scrubbing the hole with a small "pipe-cleaner" wetted with the
aqueous 70% alcohol solution or other appropriate disinfectant.

Following cleaning, leave the lids of the unit open sufficient time to
ensure that all alcohol fumes have dissipated.

MNM30016-V11 17
K-MINC-1000 INSTRUCTIONS FOR USE

4.3 Biannual Functionality Testing

IEC 601-1 Regulation
The IEC 601-1 stipulates that the user or a qualified
This functionality technician must regularly test the device to assess its
testing must be functionality and technical safety. These tests must be
performed every performed on a biannual basis. Regular inspections will
six months. assist in early detection of possible malfunctions. This
helps preserve the device and maintains its safety.
Functionality Testing
Biannually, test temperature, flow-rate and externally alarm
contacts functionality. To test temperature, thermally
equilibrate culture media in wells in the front left side of the
left chamber and the front right side of the right chamber.
To measure the temperature, immerse the tips of calibrated
thermocouples into the culture media and close the chamber
lid and allow time for the thermocouples themselves to
thermally equilibrate. The temperatures measured will be the
user set temperature ±0.1ºC. If the measured temperatures
are more than ±0.2ºC from the user set temperature for
more than two minutes, then contact your authorised service
agent. To test flow-rate, use an air calibrated gas flow meter
connected to the Luer lock outlet beneath the bacterial filter
as shown in §3.8. The gas flow meter should have no further
restriction on the outlet and be open to atmospheric
pressure. Start the gas flowing. The gas flow meter should
indicate twice the user set value ±15%. Twice the user set
value is observed because the display is a per chamber value.
An example would be to set the gas flow to 20ml/min, then
the expected result should lie between 34 to 46ml/min.
Check the flow rates over the user range of 15 to 25ml/min.
If measurements lie outside the expected ranges then contact
your authorised service agent. Gas flow rates for the K-MINC-
1000 are calibrated at 220C at sea level. Temperature and
atmospheric pressure must be taken into account when
calculating the expected gas flow. The table below shows
the flow obtained with altitude when the device has been set
at 15ml/min.

Flow variation vs Altitude

22
21
20
19
18
17
16
15
0 1 00 0 2 00 0 3 00 0 4 00 0 5 00 0 60 00

metres above sea level

To test the external alarm contacts, turn the K-MINC-1000

off using the Mains Power Switch at the rear of the unit. The
contacts should close approximately 2 minutes later. Once
contacts close, turn the K-MINC-1000 on and allow to
operate for 1 hour before opening a chamber lid and misting
the chamber with cold water. The indicated temperature
should drop and the contacts should close approximately 2
minutes later. If the contacts fail to close then contact your
authorised service agent.

MNM30016-V11 18
K-MINC-1000 INSTRUCTIONS FOR USE

4.4 Inspection by an Authorised Service Technician

WARNING:
No user serviceable parts Inspections at least once a year For ongoing operational safety of
inside! the device, it must be maintained
annually by an authorised
service technician.

Authorised service technicians All services such as alterations,

repairs, calibrations etc., may only
be performed by the authorised
service agent or by service
technicians who are authorised by
the manufacturer.

Liability The manufacturer is free from all

liability for the operational safety of
the device if the device has been
wilfully opened and unauthorised
persons have performed repairs or
alterations on it during the
warranty period.

Certification The device owner will receive a

signed certificate from the service
technician for all inspections or
repairs. This certificate states the
type and scope of the services
rendered, the service date and the
name of the service company.

Technical documentation If technical documentation is

provided by the manufacturer, this
does not authorise the user to
perform repairs, adjustments or
alterations to the device
or accessories.

Warranty Warranty will be voided should

regular maintenance not be
performed or if serviced, repaired or
otherwise modified by anyone other
than authorised personnel.

MNM30016-V11 19
K-MINC-1000 INSTRUCTIONS FOR USE

4.5 Fuse Replacement

The K-MINC-1000 may be configured to operate on one of the voltages
WARNING: listed below. Check that the voltage selector on the power entry module
ELECTRIC SHOCK is set to the correct mains voltage for your region. If the voltage is
HAZARD! changed, it may be necessary to replace the fuses to maintain electrical
Determine if the safety.
available voltage
corresponds to your
device. Connecting to
the wrong voltage will
cause the device to
malfunction or may
permanently damage
the device!

Fuses must only be

replaced with those of
appropriate rating and
To replace the fuses:-
type. Both fuses must
be of the same rating 1. Disconnect the supply cord from the supply point and then
and type! the power entry module.
2. Open the door of the module (hinge is on the lower edge).
A small screwdriver may be used.
Always remove the
mains supply cable from 3. Pull the fuse retainer drawers (there are two) from
the device before the module.
checking the fuse! 4. Unclip the old fuses from retainer drawers and
inspect the fuses.
5. Replace unserviceable fuses as required. Use only
appropriately rated replacements. (See table below).
6. Reinsert the fuse retainer drawers. The arrow should be
visible and point right when the draws are correctly inserted.
7. Observe that the desired voltage indicator is facing directly
outwards, such that it will be showing through the
door window.
8. Close the door of the module.
9. Reconnect the power cord to the power entry module and
then the supply point

Supply Voltage Suitable Voltage Selector Setting

(Vac) (V) Fuse Required

100 100 T2AL250V

115 120 T2AL250V
120 120 T2AL250V
220 220 T1AL250V
230 240 T1AL250V
240 240 T1AL250V

MNM30016-V11 20
K-MINC-1000 INSTRUCTIONS FOR USE

4.6 Return Procedure

All devices or accessory parts that are returned must be prepared as
described below for the protection of the service personnel and for safety
WARNING: during transportation.
BIOLOGICAL HAZARD!
The returned product
must be clearly marked 1. Clean as detailed in §4.2.
with a contamination 2. Seal in a plastic bag and seal within a second plastic bag.
warning and should be
sealed in a plastic bag 3. Place in the original packaging.
and sealed within a 4. Enclose the following information:
second plastic bag!
l owner's name
l owner's address
When shipping the K-
MINC-1000 ensure that l model type
the humidification flask l serial number of the equipment (see identification
and all chamber contents plate)
are removed prior to
transport! l description of the damage

When returning The authorised service agent has the right to refuse to carry out repairs if
goods, use the original the products it receives are contaminated.
packaging. The
manufacturer/authorised
service agent does not
take responsibility for
damage that has
occurred during
transportation if the
damage was caused by
inadequate transport
packaging.

MNM30016-V11 21
K-MINC-1000 INSTRUCTIONS FOR USE

5. ACCESSORIES
Order No. Description
For optimal
K-MINC-BCT-10-XXX K-MINC-1000 Braided PTFE connecting
functioning of the
hose (XXX indicates length in centimetres
incubator, use only
required). Tube length up 99 meters is
original
available.
accessories.
Standard lengths available are: 60cm,
90cm, 120cm, 150cm, 180cm & 300cm.
Lengths from 3m to 99m are special
orders and as such have an extended
delivery time.
K-MINC-CTS-S Disposable humidification flask, gas tubing
and filter set supplied.

MNM30016-V11 22
K-MINC-1000 INSTRUCTIONS FOR USE

6. TECHNICAL DATA
Classification according to IEC-601
Type of protection against electric shock: Class 1 equipment
Degree of protection against electric shock: Type B
Degree of protection against harmful
ingress of solids and water: IP31
Method of sterilisation recommended: Clean with aqueous 70% alcohol
Specifications
Power Supply 100/120/220/240VAC
Switch setting for 230Vac operation 240VAC
Switch setting for 115 Vac operation 120VAC
Fuse Rating T2AL250V (100-120VAC)
T1AL250V (220-240 VAC)
Frequency: 50-60 Hz
Maximum power consumption: 130VA
Alarm Contact Rating : 0.5A/125VAC
1A/30VDC
Environmental conditions: +10°C to +40°C
30% TO 90% relative humidity
700hPa to 1060hPa atmospheric pressure
Storage and Transport Directions: -40°C to +70°C
10% to 100% Relative Humidity
500hPa atmospheric pressure
Manufactured and tested to the following AS3200.1/IEC 601-1
standards: EN60601-1-2
FCC (part 15, subpart B, class B)
0123

MDD 93/42/EEC
Dimensions: Door closed: 405mm wide x 190mm high x 265mm
deep
Door opened: 405mm wide x 400mm high x
315mm deep
Weight: 12.5kg (27.6lb)
Gas supply type: Blend of 6% CO2, 5% O2, 89% N2 (Cook Culture
System at sea-level) or high purity 6% CO2 in air
(recommended tolerances ±0.2%)
Gas supply pressure: 150kPa ±15kPa (21.8psi±2.2psi)
(1500mbar±150mbar)
Gas supply/Flow Rate Capability : 15mL/min-25mL/min per chamber in 5mL/min
increments.
Purge at 180mL/min per chamber for 3 minutes.
Chamber temperature capability: 35-40°C in 0.1°C increments (at ambient
temperature of 20-30°C. For set point of 37°C
ambient operational range is extended to 18-32°C
Chamber temperature accuracy: ±0.2°C

MNM30016-V11 23
K-MINC-1000 INSTRUCTIONS FOR USE

7. TROUBLESHOOTING
Should any errors persist, contact your Cook distributor.

Error and Source of Error Elimination of Error

Alarm
Indicator
Unit will not Power supply cord is not Check the power supply connection. Turn on the
turn on. connected. Mains power mains power switch. (Refer to §3.9)
Displays do switch is not turned on.
not illuminate.

Incorrect voltage. Check supply voltage and the selected voltage on the
rear of the device. (Refer to §3.5)
Fuse is defective. Replace the fuse. (Refer to §4.5)

Incubator will Temperature settings not Enter correct temperature settings.

not heat to entered correctly. (Refer to §3.8)
correct
temperature.
‘Temperature Set temperature not Turn heater bay off and on again.
out of range’ achieved in time. (Refer to §3.8)
alarm "ERR"
display.
Lids open for extended Do not leave lids open for extended periods. Turn
periods heater bay off and on again.
(Refer to §3.8)

‘Gas supply Gas supply not connected Check gas supply connection. Turn flow off and on
out of range’ again. (Refer to §3.7.3)
alarm
"ERR" display Gas supply empty Check available remaining volume. Turn flow off and
or "CO2" on again.
display.
Gas lines are kinked. Check that gas lines are not obstructed. Turn flow off
and on again.
Disposable gas lines, Check connections to Luer fitting in central chamber
lask and filter are incorrectly and check that disposable lines are not kinked. Turn
connected or occluded. flow off and on again. (Refer to §3.8)

Disposable filter occlusion Replace the filter. Turn flow off and on again.
due to moisture. (Refer to §4.1)

Disposable gas lines are Check that gas lines are not kinked or obstructed.
kinked or blocked. Turn flow off and on again.
Gas change over unit Ensure that the inlet pressure to the K-MINC-1000
incompatible with does not fall below 135kPa. Turn flow off and
K-MINC-1000. on again.

MNM30016-V11 24
K-MINC-1000 INSTRUCTIONS FOR USE

Error and Source of Error Elimination of Error

Alarm
Indicator
Low or no gas Disposable flask and filter Check connections to Luer fitting in central chamber.
flow from are incorrectly connected. (Refer to §3.8)
disposable gas
lines without
alarm present.
Disposable flask lid seal. Check lid is tight and flask is not cracked or
damaged. (Refer to §3.8)

Excessive gas High supply pressure. Ensure that the inlet pressure to the K-MINC-1000
consumption. does not rise above 165kPa.

Gas connections not secure Ensure all gas fittings from the bottle to the K-MINC-
or damaged 1000 gas inlet are tight. If error persists, inspect all
fittings for damage to seal surfaces, and replace as
necessary.
Gas line damaged. Replace the gas line.

0.1s audible Exhausted battery Unit must be returned to authorised service agent for
beep heard power-fail external alarm) battery replacement (Refer to §3.14.4 and §4.6)
every 15
seconds while
incubator
operating

MNM30016-V11 25
K-MINC-1000 INSTRUCTIONS FOR USE

8. LIMITED WARRANTY
COOK AUSTRALIA warrants to the purchasers of this device that at time of
manufacture, the product was prepared and tested in accordance with
good manufacturing practices and guidelines specified by the Australian
Therapeutic Goods Administration or relevant competent authority.
In the event of product failure under normal use, due to defects in
material or workmanship, within a period of one (1) year from the date of
purchase, the product will be repaired, or at Cook’s option, replaced, at
no charge. This limited warranty does not apply to products subjected to
abnormal use or conditions, improper storage, damaged by accident,
misuse or abuse, improper line voltage or to products altered or serviced
by anyone other than Cook Australia or its authorised agent.
The foregoing limited warranty is exclusive and in lieu of all other
warranties whether written, oral, expressed or implied. In particular, Cook
Australia does not warrant that the product is suitable for the needs of the
purchaser and there are no warranties given as to merchantability or
fitness for a particular purpose. Cook Australia’s representations
concerning fitness for purpose or suitability for use by any purchaser does
not extend beyond those representations set out in the Cook Australia
literature that accompanies the product. Cook Australia assumes that the
purchaser is experienced in the use of this device and is able to judge
from his/her own expertise the suitability or otherwise of the product for
the intended use. Cook Australia conducts a technical advisory service,
which can be consulted by a purchaser or intended purchaser on an
advisory basis.
Before returning a product for any reason, please contact your nearest
Cook distributor for assistance and instructions.
Cook Australia reserves the right to change or discontinue this product
without notice.

8.1 Liability
Because Cook Australia has no control or influence over the conditions
under which this device is used, over its method of use or administration,
or on handling of the product after it leaves its possession, Cook Australia
takes no responsibility for the results, use and/or performance of the
product. Cook Australia expects that use of the product will be confined
to trained and expert users.
In no event will Cook Australia be liable for any direct or indirect damages
including incidental, consequential or special damages, arising out of or
in connection with the use or performance of the product.
If the manufacturer provides you with technical documentation, this does
not authorise you to perform repairs, adjustments or alterations on the
device or accessories.
No representative of Cook Australia and no vendor or lessor of the
product is authorised to change any of the foregoing terms and
conditions, and the purchaser accepts the product subject to all terms
and conditions herein, subject always to any contrary provisions which are
necessarily implied by stature or law notwithstanding the within terms
and conditions.

MNM30016-V11 26
K-MINC-1000 INSTRUCTIONS FOR USE

8.2 Life of Product

The life of this product is deemed to be seven years. After this time Cook
Australia will no longer be responsible for the support or maintenance of
the product. The local Service Provider may still perform repairs or
maintenance.

MNM30016-V11 27
K-MINC-1000 INSTRUCTIONS FOR USE

9. INDEX
A F
About the K-MINC-1000, 2 Filter, 12
Accessories, 22 First Time Use, 14
Activating the Device, 13 Flow rate capability, 23
Alarm Conditions, 15 Four-well dishes, 14
Alarm contact rating, 23 Frequency, 23
Alarm Indicator, 24, 25 Front of the Device, 5
Alter the temperature settings, 13 Functionality testing, 18
Authorised service technicians, 19 Fuse Rating, 23
Automatic purge, 13 Fuse Replacement, 20
B G
Biannual Functionality Testing, 18 Gas Bottle Regulator Recommendations, 9
Blanking plug, 10 Gas Flow Rate Display, 5
Gas Flow Rate Selection, 14
C Gas Flow Rate Selection Touch-pads, 5
Certification, 19 Gas Flow Start/Stop Touch-pad, 5
Chamber temperature capability, 23 Gas Flow Status Indicator, 5
Chamber Temperature Selection, 13 Gas inlet, 6
Change over units, 11 Gas Outlet (blocking plug shown), 6
Changing Internal Filter, Gas Lines and Gas line, 12
Humidification Flask, 16 Gas Mixture Required, 8
Checklist, 2 Gas regulator, 9
Classification according to IEC-601, 23 Gas Supply, 8, 23
Cleaning the Device, 16 Gas supply pressure, 23
Concentration of CO2, 8 Gas supply type, 23
Connecting hose, 10 Gas Vent Holes, 6
Connection to Gas Bottle, 10 Grounding, 7
Contra-indication for Device Use, 2
H
Cook-Net Connections, 6
Heating Status Indication, 5
D
How to use this Manual, iv
Degree of protection, 23 Heating Start/Stop Touch Pads, 5
Device Placement, 8 Humidification Flask, 12
Dimensions, 23
Disposable humidification flask, 12 I
E ICSI workstation, 14
Electric shock hazard, 1 IEC 601-1 regulation, 18
Elimination of Error, 24, 25 Insertion of the Culture Dishes, 14
Environmental conditions, 23 Inspection by an Authorised
ERR display, 15,24 Service Technician, 19
Excessive gas consumption, 25 Inspections at least once a year, 19
Explanation Of Pictograms, iii Installation and Set-up, 3
Explosion Hazard, 1 Intended Use, 2
External Alarm, 15 Items not supplied, 4
External alarm monitor, 15 Items supplied, 3
External alarm contacts, 6
K
K-MINC-BCT-10-XXX, 22

MNM30016-V11 28
K-MINC-1000 INSTRUCTIONS FOR USE

L T
Liability, 19, 26 Technical Data, 23
Limited Warranty, 26 Technical documentation, 19
Loss of mains power, 15 Temperature display, 5
Low Battery (power-fail external alarm), 15 Temperature out of range, 15
Low or no gas flow, 25 Temperature selection touch pads, 5
Temperature selection, 13
M
To replace the fuses, 20
Mains Power Indication, 5 To test flow-rate, 18
Mains Power Switch, 6 To test temperature, 18
Mains voltage, 7 Troubleshooting, 24
Manual Structure, iv Type of protection, 23
Media, 8
Method of sterilisation, 23 U
Multiple K-MINC-1000 devices, 11 Unpacking, 3
N V
No Gas Flow Out of Range, 15 Voltage selection, 7
NUNC® four-well dishes, 14 Voltage selector, 7
O W
Original accessories, 1 Warnings, Cautions, and Important Notes, iv
Other Gas Connections, 11 Weight, 23
Oxygen concentration, 8 White board, 14
Within the U.S.A, 7
P
Petri dishes, 14 Y
Pictograms, iii You Need to Supply, 4
Power consumption, 23
Power entry module, 6
Power On/StandbyTouch-pad, 5
Power Supply, 23
R
Rear of the Device, 6
Regular inspections, 18
Return Procedure, 21
S
Safety Instructions, 1
Safety plug, 7
Sequential connection, 11
Series Connections of Devices, 11
Service and Maintenance, 16
Specifications, 23
Storage and transport directions, 23
Supply Voltage Selection, 7
SWAGELOK® 1/4" series tube fittings, 10

MNM30016-V11 29
K-MINC-1000 INSTRUCTIONS FOR USE

10. INSTALLATION AND SET-UP CHECKLIST

Check the following:-

o All items have been supplied.

o The packaging has been safely stored for future use.

o All non-sterile items have been removed from plastic covers.

o The voltage selector is correct for your region.

o The fuses are correct rating for your region.

o The device has been placed in a suitable location.

o The appropriate gas mixture has been determined.

o An appropriate gas regulator has been sourced.

o The gas regulator has been set to 150kPa.

o The gas connections have been made and checked.

o The humidification flask has been filled and fitted.

o The device has been activated.

o The chamber temperatures and flow rate have been adjusted to desired values.

o The device has been left to operate for 24 hours to ensure any residual out-gassing
of components is complete.

MNM30016-V11 30
NOTES
PRODUCT MANUFACTURED BY: COOK AUSTRALIA PTY LTD
A Division of William A Cook
Australia Pty Ltd
ABN 79 005 526 723
12 Electronics Street
Brisbane Technology Park
Eight Mile Plains
Queensland 4113 AUSTRALIA
Telephone: +61 7 3841 1188
Fax: +61 7 3841 1288

EC REPRESENTATIVE: COOK IRELAND LTD

O’Halloran Road
National Technology Park
Limerick IRELAND
Telephone: +353 61 33 4440
Fax: +353 61 33 4441

NORTH AMERICAN REPRESENTATIVE: COOK OB/GYN

1100 West Morgan Street
Spencer Indiana 47460 USA
Toll Free: 800 541 5591
Telephone: +1 812 829 4891
0123

Fax: +1 812 829 2022

www.cookgroup.com