Labgard Class II, Type A2

Laminar Flow
Biological Safety Cabinet

Model
NU-425-200
NU-425-200E
NU-425-200G
Bench/Console

Operation & Maintenance Manual

June, 2005
(Series 30 and Higher)
Revision 2

(115V Only) (115V Only)

Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: (763)-553-0459

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Congratulations!

You have just purchased one of the finest Laminar Flow Biological Safety Cabinets available. With
proper care, maintenance (certification), and laboratory procedure, this cabinet will give you years of product
and personnel protection from particulate contaminants as prescribed in NSF/ANSI 49:2002. Please read this
manual carefully to familiarize yourself with proper installation, maintenance and operation of the cabinet.

Acknowledgment
NuAire, Inc. acknowledges that some material in this manual reflects information supplied by the National Institutes of Health personnel both in
verbal and written specifications. In particular, NuAire acknowledges that information in Selection 8 was obtained from the following sources:

1. Technical Report No. FPS 56500000001. Prepared by Dow Chemical Co., for the National Cancer Institute, May 1, 1972.

2. Stolar MH, Power LA, Vielo CS: Recommendations for handling cytotoxic drugs in hospitals. Am J Hosp Pharm 1983;40: 1163-1171.

3. Anderson R.W., Director of Pharmacy, University of Texas, M.D. Anderson Hospital and Tumar Institute at Houston.

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 ABOUT THIS OPERATION & MAINTENANCE MANUAL

The information contained in this manual is intended to reflect our current production standard configuration
model along with the more frequently purchased options. Any unique additions/modifications/shop
drawings are appended in the back flap of this manual, along with any modifications and/or additions to
procedures as outlined in this manual. A copy of the original factory test report is also appended to this
manual. In case this manual and/or test report is lost or misplaced, NuAire retains a copy in our files. A
replacement copy can be obtained by calling or writing NuAire, Inc. stating the model number and serial
number and a brief description of the information desired.

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 Labgard Class II, Type A2
Laminar Flow Biological Safety Cabinet
Model
NU-425-200
NU-425-200E
NU-425-200G
Operation & Maintenance Manual

TABLE OF CONTENTS

Section No. 1 .................................................General Description

Section No. 2 .................................................Models & Options
Section No. 3 .................................................Warranty
Section No. 4 .................................................Shipments
Section No. 5 .................................................Installation Instructions
5.1.................................................................. Location
5.2.................................................................. Set-up Instructions
5.3.................................................................. Certification Testing Methods and Equipment
Section No. 6 .................................................Operating the NU-425-200
6.1.................................................................. Operator Controls & Indicators
6.2.................................................................. Operating Guidelines
6.3.................................................................. Operating Sequence
6.4.................................................................. Ergonomics
6.5.................................................................. Cleaning Procedures
6.6.................................................................. Antineoplastic Decontamination Procedure
Section No. 7 .................................................General Maintenance
7.1.................................................................. Decontamination
7.2.................................................................. Fluorescent Lamp Replacement
7.3.................................................................. HEPA Filter/Motor Replacement
7.4.................................................................. Sliding Window Replacement & Adjustment
7.5.................................................................. Airflow Calibration
7.6.................................................................. Filter Integrity Check
7.7.................................................................. Cleanliness Classification Test For Pharmacy Application
Section No. 8 ............................................................... Error Indicatiors & Troubleshooting
Section No. 9 ............................................................... Remote Contacts
Section No. 10 ...............................................Optional Equipment
10.1................................................................ Ultraviolet Light
Section No. 11 ............................................................. Electrical/Environmental Requirements
Insert ...............................................Replacement Parts List

MANUAL DRAWINGS
BCD-09324..................................... NU-425-200 Airflow Schematic
BCD-09325..................................... NU-425-200 Specification Drawing
BCD-05322..................................... Front Panel Controls & Indicators

ASSEMBLY DRAWINGS
BCD-09328..................................... Base Stand Assembly NU-425-200
BCD-09326..................................... HEPA Filter/Motor Replacement

ELECTRICAL SCHEMATICS
BCD-09323..................................... NU-425-200 Electrical Schematic
BCD-09327..................................... NU-425-200 E,G Electrical Schematic

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 Labgard Class II, Type A2
Laminar Flow Biological Safety Cabinet
Models
NU-425-200
NU-425-200E
NU-425-200G
MANUFACTURED BY:
NuAire, Inc. - Plymouth, Minnesota, U.S.A.

1.0 General Description

The LABGARD Model NU-425 Laminar Flow Biological Safety Cabinet (LFBSC) is a bench/table
top model, optionally available with a base support stand, for operation as a console model.

The Laminar Flow Biological Safety Cabinet, (LFBSC) is a product resulting from the development
of the "laminar flow" principle and the application of environmental controls as required in the field of
biological research or chemical containment. The LFBSC, when used with proper technique, is an effective
laboratory aid in obtaining the optimum control over product quality while reducing the potential for
exposure of both product and personnel to airborne biological or particulate chemical agents in low to
moderate risk-hazard research and drug preparation or product operations, as prescribed by the Center for
Disease Control (CDC) Atlanta, Georgia.

The NU-425 bench LFBSC is known as a Class II, Type A2 Biological Safety Cabinet. This is
possible since NuAire's cabinet conforms to the following requirements:

1. Maintains inflow velocity of 100 LFPM (.51 M/S) through the work access opening.

2. Has HEPA filtered downflow air that is mixed with the inflow air from a common exhaust
plenum.

3. Exhaust airflow can either be room re-circulated or exhausted outside using a canopy
exhaust transition.

4. Has all biologically contaminated ducts and plenums under negative pressure or surrounded
by negative pressure. (Type A1 permits positive pressure contaminated ducts and plenums).

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OM0140 6
2.0 Models & Features
The model NU-425-200 is a Class II, Type A2 Laminar Flow Biological Safety Cabinet.

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OM0140 8
3.0 Warranty
NuAire, Inc. warrants that it will repair F.O.B. its factory or furnish without charge F.O.B. its factory
a similar part to replace any material in its equipment within 36 months after the date of sale if proved to the
satisfaction of the company to have been defective at the time it was sold provided that all parts claimed
defective shall be returned, properly identified to the company at its factory, charges prepaid. Factory
installed equipment or accessories are warranted only to the extent guaranteed by the original manufacturer,
and this warranty shall not apply to any portion of the equipment modified by the user. Claims under this
warranty should be directed to NuAire, Inc. setting forth in detail the nature of the defect, the date of the
initial installation and the serial and model number of the equipment.

This warranty shall not apply to any NuAire product or part thereof which has been subject to
misuse, abuse, accident, shipping damage, improper installation or service, or damage by fire, flood or acts
of God. If the serial number of this product is altered, removed or defaced as to be illegible, the Warranty
shall be null and void in its entirety.

The warranty is for the sole benefit of the original purchaser and is not assignable or transferable.
Prior to returning any item, for any reason, contact NuAire for a Return Authorization Number. This number
must accompany all returns. Any product shipped to NuAire without this number will be returned refused
shipment or collect freight.

4.0 Shipments

NuAire takes every reasonable precaution to assure that your LABGARD cabinet arrives without
damage. Motor carriers are carefully selected and shipping cartons have been specially designed to insure
your purchase. However, damage can occur in any shipment and the following outlines the steps you should
take on receipt of a NuAire LABGARD cabinet to be sure that if damage has occurred, the proper claims and
actions are taken immediately.

4.1 Damaged Shipments

4.1.1 Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment
before acceptance.

4.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on
the consignee's copy of the freight bill. Then an inspection must be made to verify the claim
against the carrier. This inspection is the basis of your filing the claim against the carrier.

4.1.3 If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT

AGENT AT ONCE, and request an inspection. Without this inspection, the transportation
company may not accept a claim for loss or damage. If the carrier will not perform the
inspection, an affidavit must be prepared stating that he was contacted on a certain date and
that he failed to comply with the request. This along with other papers in the customer's
possession will support the claim.

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5.0 Installation Instructions
5.1 Location
Within the laboratory, pharmacy, etc., the ideal location of the biological safety cabinet is away from
personnel traffic lanes, air vents (in or out), doors and/or any other source of disruptive air currents.

If drafts or other disruptive air currents exceed the intake velocity of the cabinet through the access
opening, the potential exists for contaminated air to exit or enter the work tray area of the cabinet. It
depends on the severity of the air current. REMEMBER: A BIOLOGICAL SAFETY CABINET
IS NO SUBSTITUTE FOR GOOD LABORATORY TECHNIQUE.

Where space permits, a clear twelve (12) inch area should be permitted on each side of the cabinet
for maintenance purposes. The electrical outlet into which the cabinet is connected should be readily
accessible for maintenance purposes. A MINIMUM CLEARANCE OF 6" (152MM) IS REQUIRED
FROM THE TOP OF THE CABINET TO THE CEILING FOR PROPER VENTILATION OF THE
EXHAUST EFFLUX. HOWEVER, FOR CERTIFICATION OR COMMISSIONING, 18 INCHES
(451MM) IS REQUIRED TO OBTAIN VALID EXHAUST MEASUREMENTS.

If this cabinet is used in a pharmacy application, Per OSHA, NIOSH, and ASHP, it is strongly
recommended that the cabinet be exhausted to the outside. In addition, if this cabinet is used in
microbiological application with minute quantities of volatile toxic chemicals and tracer amounts of
radionuclides, Per CDC/NIH and NSF it is strongly recommended that the cabinet be exhausted
to the outside. NuAire offers two general categories of exhaust transitions, which will capture the
exhaust efflux from the cabinet. These are:

Canopy, Thimble or Air Gap Exhaust Transitions (with and without integral fan)
Gas-Tight Exhaust Transitions

CAUTION: THE EXHAUST SYSTEM SHOULD BE FITTED WITH A BACKDRAFT

DAMPER TO PREVENT REVERSING OF AIRFLOW IN THE SYSTEM.

Both types of transitions have some common attributes, in addition to some that are unique. NuAire
strongly recommends a canopy or thimble exhaust for most applications. See separate
instruction sheets for a discussion of exhaust transitions and installation requirements.

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5.2 Set-Up Instructions
Remove outer shipping protection (carton or crating). The cabinet is fastened to the base skid and it
is usually the best procedure to leave the skid in place until the cabinet is located in its approximate
position to facilitate ease in handling. It can then be removed from the skid by removing the banding
holding the cabinet to the skid and unbolting the strap from the front flange to the base skid.

5.2.1 Base Stand Assembly

The base stand is shipped K.D. in a separate carton and is assembled per drawing BCD-
02409 if accompanied with the unit. In order to insure that the base stand is dimensionally
stable, the following procedure must be followed.

1. Assemble end stands (2), cross members (2), and rear corner brackets (2) as shown
in Isometric A, B and C (Please note rear corner bracket orientation).
2. Place the base stand in its final desired position and loosely attach the floor
mounting anchor brackets.
3. Mark the floor anchor bracket hole locations and drill pilot holes for appropriate
anchor type (1/2" diameter x 1 1/4" deep for concrete floor type -- 11/64 diameter
for 1/4" lag screws).

4. Fasten the anchor bracket to the floor, while leaving the anchor bracket loosely
attached to the end frames.

5. Using a level place on the base stand crossmembers, adjust the leg levelers, first end-
to-end, then front-to-back. The leg levelers provide ± 3/4-inch adjustment.

6. Tighten floor anchor bracket to end stand.

7. Lift the cabinet onto the rear corner brackets.

8. Fasten the front corner bracket to the end stand and the cabinet as shown in
Isometric A.

9. Install the drain valve on the bottom right front of the cabinet, using Loctite 242
(furnished) to the threads and rotate the valve body until it is secure.

5.2.2 Bench Installation

Place the cabinet on the bench with approximately a two (2) inch (51mm) overhang
clearance for installation of the drain valve. If the drain valve is not desired, place the
cabinet in its desired location and using RTV caulk, seal all around the base of the cabinet
and the bench. this provides a tight seal to prevent bench spills from migrating under the
cabinet.

If a drain valve is desired, remove the handle from the valve stem to gain clearance for valve
body rotation. Add Loctite 242 (furnished) to the threads and rotate valve body until secure,
with the valve stem (for handle) on the left side. Re-install handle to valve stem. Adjust the
cabinet on bench to provide a 1-1/2 inch (38mm) overhang and seal the interface of the
bench and cabinet, using RTV caulk as above.

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 5.2.3 Gas Service
NuAire doesn't recommend the use of natural gas within the BSC, but if gas service is
determined to be necessary for the application, appropriate safety measures must take place.
All NuAire BSC's have precautionary warning labels that say the following:
CAUTION
Use of toxic, explosive or flammable substances in this cabinet
should be evaluated by your appropriate safety personnel.

Once the determination has been made by the appropriate safety personnel, the application
of natural gas must be performed in accordance to national, state and local codes.
IT IS ALSO STRONGLY RECOMMENDED THAT AN EMERGENCY GAS
SHUTOFF VALVE BE PLACED JUST OUTSIDE THE BSC ON THE GAS SUPPLY
LINE.

All NuAire BSC's meet the safety requirements of UL and CSA for Laboratory Equipment.
To comply with these safety requirements, NuAire uses only certified gas valves. In
addition, if external piping is required, only black pipe is used for this application.

As previously stated, NuAire doesn't recommend the use of natural gas within the BSC and
ASSUMES NO RESPONSIBILITY FOR ITS USE. USE AT YOUR OWN RISK. The
Bunsen burner flame within the BSC not only contributes to heat build-up; is also disrupts
the laminar air stream, which must be maintained for maximum efficiency. IF THE
PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON
DEMAND IGNITION IS STRONGLY RECOMMENDED. DO NOT USE
CONSTANT FLAME GAS BURNERS. During use, the Bunsen burner should be placed
to the rear of the workspace where resulting air turbulence will have a minimal effect.

5.2.4 Plumbing Services (Optional)

Ground key cocks with the type of service specified by the removable button on the handle,
are located in the work zone. The Ground Key cocks are not recommended for pressure over
30 p.s.i (2.0 Bar). Reducing valves should be installed external to the cabinet if necessary.
Ground key cocks should never be used for flammable gasses or oxygen service. A special
needle valve for oxygen service or certified valve is required and available upon request.

External connection is to 3/8 inch NPT coupling in the inner side walls. Connection to plant
utilities should be made with proper materials for the individual service and according to
national and/or local codes. Observe all labels pertaining to the type of service and
operating pressure.

5.2.5 Electrical Services

The NU-425 series Biological Safety Cabinets may be "hardwired" (optional) or connected
via an electrical power cord which is standard. The unit requires 115, 220 or 230 VAC
50/60 Hz, single phase (current rating varies per cabinet size, reference
Electrical/Environmental Requirements). It is recommended that power to the unit be on its
own circuit, protected with a circuit breaker or fuse at the distribution panel.

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 5.2.6 Final Assembly
REMOVE THE PROTECTIVE CARDBOARD COVER OVER THE EXHAUST
HEPA, located under the protective screen. The exterior surface and viewing glass are
easily cleaned with any mild household detergent cleaner using a soft cloth. Harsh
chemicals, solvent-type cleaners and abrasive cleaners should not be used.

Do not attempt to clean the HEPA filter media. Cabinet interior walls or work
surface are easily cleaned with any mild household detergent cleaner using a soft
cloth. Turn the cabinet on and let it operate for 60 minutes before using it as a LFBSC.

5.3 Certification
After installation prior to use, NuAire recommends that the cabinet be recertified to factory
standards. At a minimum, the following tests should be performed.

1. HEPA filter leak test

2. Downflow velocity test
3. Inflow velocity test
4. Airflow smoke patterns

The testing methods and equipment required are specified on the factory inspections report included
with this manual (see insert in back cover).

IT IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED

TECHNICIAN WHO IS FAMILIAR WITH THE METHODS AND PROCEDURES FOR
CERTIFYING BIOLOGICAL SAFETY CABINETS (SEE INSERT).

AFTER THE INITIAL CERTIFICATION, NUAIRE RECOMMENDS THAT THE

CABINET BE RECERTIFIED AT A MINIMUM ON AN ANNUAL BASIS AND AFTER
EVERY FILTER CHANGE OR MAINTENANCE ACTION OR ANY TIME THE
OPERATOR FEELS IT IS NECESSARY.

Note that the LABGARD cabinets, filters and seals provide premium performance; Quality Control
in both design and manufacturing assure superior reliability. However, protection to both product
and operator is so vital that certification to the performance requirements should be accomplished as
stated to ensure biological safety established by the factory standards.

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6.0 Operating the NU-425
6.1 Operator Controls & Indicators
The following is a description of the controls and indicators found on both the front panel (see
Drawing BCD-05322) and cabinet.

6.1.1 Circuit Breaker-Blower (Left side of Control Center)

The motor/blower is protected with a circuit breaker. The circuit breaker in conjunction with
the motor's thermal protector is designed to open under locked rotor or half-wave power
conditions. Should the circuit breaker open (pop-out button will appear) merely depress to
reset. If the circuit breaker continually opens, a failure has occurred in the motor or solid-
state speed controller. Consult a qualified repair technician or NuAire, Inc. for replacement.

6.1.2 Circuit Breaker-Outlets (Left side of Control Center)

The duplex outlet located in the sidewall of the work area is protected with a 3 amp circuit
breaker. The circuit breaker may trip at 110% of load rating but will trip at 145% of load
rating in less than 2 seconds. Should the circuit breaker open, (pop-out button will appear),
unplug the appliance plugged into the outlet and merely depress the pop-out button to reset.

6.1.3 Fluorescent/U.V. Light Switch

This switch provides on/off control for the fluorescent light and/or the ultraviolet (U.V.)
light if present (optional). With the U.V. light option, the switch provides for on-center off-
on operation so that both the fluorescent light and the U.V. light cannot be energized at the
same time. The UV light is also interlocked, so it will only operate if the sliding window is
closed. Proper care should be exercised when the U.V. light is on.

6.1.4 Outlet Switch

This switch provides on/off control for the 115 VAC power available in the outlet(s) within
the cabinet workzone.

6.1.5 Blower Switch

The blower switch applies power to the internal motor/blower when in the ON position. The
blower switch also has a second set of poles, which are available for use as a contact closure
for an exhaust system. In addition, power can be applied to the accessory outlet located on
the upper left corner in back of the control center.

The purpose of the outlet is to provide "switched" power for the following:

1) Exhaust transition with integral blower

2) To power a low power appliance, such as the low airflow alarm.

The rating of the outlet is 115 VAC at 1 amp maximum.

6.1.6 Indicator Light

An indicator light is located above the Blower on/off switch and indicates when power is
applied to the blower.

6.1.7 Audible Alarm Enable

As the sliding window is raised above its normal working height, a micro switch located in
the glide channel activates both an audible and visual alarm. This switch disables the
audible alarm but will not disable the red indicator visual alarm.

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OM0140 15
 6.1.8 Airflow Control
The operating airflows within the cabinet (i.e. 70 LFPM (.35 m/s) downflow and 105
LFPM (.53 m/s) air inflow barrier) are controlled by a potentiometer and an exhaust
damper. The potentiometer controls the operating voltage applied to the
motor/blower. The potentiometer is adjustable over 270 degrees with a slotted
screwdriver, which varies the applied voltage from 70 to 115 VAC. THIS
ADJUSTMENT SHOULD ONLY BE MADE BY A QUALIFIED TECHNICIAN
EMPLOYING THE PROPER INSTRUMENTS IN ORDER TO INSURE
AIRFLOWS PER NSF STD. NO. 49.

6.1.9 Minihelic Gauge

The unit is equipped with a minihelic gauge. The minihelic gauge displays the static
pressure within the pressure plenum supplying the downflow and exhaust filters. The
gauge is calibrated in "inches of water gauge" pressure. As the HEPA filters load with
particulate matter, the amount of static pressure will increase, giving and indication of
the "health" of the cabinet. The initial pressure reading will be approximately 0.5"
w.g. + 0.1" w.g. depending on altitude from sea level. At each 0.1" w.g. increment
increase, the cabinet airflow should be checked by a qualified technician, unless
certified on a yearly (or sooner) basis.

6.1.10 Sliding Window Operation

The cabinet has a full counter balanced and removable sliding tempered glass window
with two operational features. As the window is raised above its specified operating
height, an audible alarm alerts the operator of possible compromised personnel
protection. At the same time, an audible and visual alarm is activated. The audible
alarm may be switched off while the visual alarm remains until lowered to the safe
operating position. When the window is lowered below 2 inches (50mm), the
motor/blower automatically shuts down, to prevent stress on the motor/blower in the
form of overheating. The maximum window height is 19 1/2 inches (495mm).

6.1.11 Convenience/Accessory Outlet

A convenience/accessory outlet is located in the upper left hand corner in back of the
control panel. The outlet is normally wired "hot" through the motor/blower circuit
breaker. If an accessory is purchased, the outlet is defined as an accessory outlet
supplying 115 VAC at 1.0 Amp maximum, and is wired to the blower switch (see
electrical schematic).

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6.2 Operating Guidelines
The intent herein is to present general operational guidelines that will aid in the use of the
Laminar Flow Biological Safety Cabinet (LFBSC) to control airborne contaminants of low to
moderate risk as stated in Technical Report No. FPS 56500000001 prepared by Dow Chemical
U.S.A. for the National Cancer Institute, May 1, 1972.

Procedure protocols defined in terms of the barrier or control concepts unique to LFBSC must
be developed in order to obtain a maximum potential for safety and protection. The pre-
planning necessary to develop these protocols is based on several fundamental considerations,
each of which will contribute to optimum benefits from the equipment:

a. Know you "Safe Work Area"

b. Minimize disruption of "air curtain"
c. Minimize room activity
d. Utilize unidirectional air flow
e. Employ aseptic techniques

6.2.1 Know Your "Safe Working Area"

The LFBSC safe working area is basically the worktray or depressed area. All work
should be performed on or above the worktray. The area on or above the front grill is
a non-safe working area.

6.2.2 Minimize Penetration of "Air Curtain"

The minimum number of items necessary should be placed into the cabinet to prevent
overloading, but the work should also be planned to minimize the number of times an
operator's hands and arms must enter and leave the air curtain at the open face. The
ideal situation is to have everything needed for the complete procedure placed in the
hood before starting, so that nothing need pass in or out through the air barrier at the
face until the procedure is completed. This is especially important in working with
moderate risk agents.

Unnecessary raising of the hands inside the cabinet above the level of the work opening
should be avoided. This presents an inclined plane from hands to elbows along which
the downflow of air may run to, and possibly out, the open face.

Note: When working with agents of lower risk, it is not as important for all materials to be
placed in the cabinet before starting, or for the procedure to be completely finished
before materials are removed. Also, the time period for a unit may be continued over
a more extended period during which entries and withdrawals from the cabinet may be
made.

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 6.2.3 Minimize Room Activity
Activity in the room itself should be held to a minimum. Unnecessary activity may
create disruptive air currents as well as interfere with the work of the operator. A
person walking past the front of a cabinet can cause draft velocities up to 175 fpm,
which are sufficient to disrupt the air balance of the laminar flow unit.

6.2.4 Utilize Unidirectional Air Flow

The operator must keep two important facts in mind: (1) The air, as supplied to the
work area through filters from the top, is contaminant free and (2) Airborne
contamination generated in the work area is controlled by the unidirectional flow of
parallel air streams in a top-to-bottom direction.

A solid object placed in a laminar air stream will disrupt the parallel flow and
consequently, the capability of controlling lateral movement of airborne particulates.
A cone of turbulence extends below the object and laminarity of the air stream is not
regained until a point is reached downstream, approximately equal to three to six times
the diameter of the object. Within the parameters of this cone, particles may be
carried laterally by multidirectional eddy currents.

Transfer of viable materials and manipulations which may generate aerosols should
not be performed above sterile or uninoculated materials. Items should be localized
on the work surface in "clean" and "dirty" groups.

6.2.5 Employ Aseptic Technique

The operator must not assume an attitude of "let the cabinet do it" when performing
procedures within a LFBSC. Properly balanced and properly used cabinets will do an
excellent job of controlling airborne contamination and containing viable agents, but
the cabinet will not eliminate contact transmission of contamination. Normal
laboratory contamination control procedures and basic aseptic techniques are
necessary to obtain maximum benefit from the cabinet. For example, open bottle, tube
or flask mounts should be kept as parallel as possible to the downflow to minimize
capture of chance particulates. This precaution is merely an extension of good aseptic
technique as practiced on open bench tops. The good laboratory practices designed to
minimize creation and/or release of aerosols to the environment should not be
discontinued.

Items of equipment in direct contact with the etiologic agent must remain in the
cabinet until enclosed or until surface-decontaminated. Trays of discard pipettes must
be covered before removal from the cabinet (aluminum foil may substitute for
fabricated covers).

If an accident occurs which spills or splatters suspensions of etiologic agent around

the work area, all surfaces and items in the cabinet must be surface-decontaminated
before being removed.

Applying a burner flame to flask and tube necks when mating surfaces of sterile
assemblies is a conventional method of minimizing chance contamination. However,
the efficiency of this operation is usually related to the removal of airborne
contamination occurring while the item is uncovered. If the manipulation is carried
out in an environment free of airborne particulates, then the need for the flaming
operation is essentially removed. This is one of the additional advantages of the
LFBSC - use of the gas burner is seldom necessary. The gas burner flame in one of
these units not only contributes significantly to the heat build-up, it also disrupts the
laminar air streams which must be maintained for maximum efficiency.

OM0140 18
 IF THE PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER
WITH ON DEMAND IGNITION IS RECOMMENDED. DO NOT USE
CONSTANT FLAME GAS BURNERS. It should also be placed to the rear of the
work space where resulting air turbulence will have a minimal effect. If cabinet air is
inadvertently turned off, the flame could damage the HEPA filters.

6.3 Operating Sequence

6.3.1 Start Up
Turn on cabinet blower and lights, check air intake and exhaust portals of the cabinet
to make sure they are unobstructed. Many of the cabinets are provided with
magnehelic gauges which indicate pressure differentials across the supply filters.
They may indicate to the LFBSC maintenance technician when to replace the filters,
dependent upon the blower fan capacity. Blower speed must only be readjusted by
qualified maintenance technicians.

Note: Some cabinets are equipped with ultraviolet (UV) lights. These must be turned off
during the day while laboratory personnel are occupying the room. Good procedure
includes the decontamination or wipedown of cabinet surfaces with chemical
disinfectant before work commences. This practice eliminates the need for UV lights,
whose primary utility in this application is inactivation of surface contamination since
the filters effectively remove all airborne contaminants. UV lights, therefore, are not
recommended in the LFBSC.

Allow blowers to operate for a minimum of 15 minutes before aseptic manipulations

are begun in the cabinet. If the filtered air exhausted from the unit is discharged into
the room, as in some installations, an additional advantage is obtained from
purification (filtration) of the room air circulated through the equipment. Because of
this characteristic contributing to the quality of the laboratory environment, some
owners of LFBSC leave them in operation beyond the time of actual use.

6.3.2 Wipedown
The interior surfaces of the work space should next be disinfected (see cleaning
procedures) by wiping them thoroughly with 70% alcohol or similar non-corrosive
antimicrobial agents. DO NOT USE ANY CHLORINATED OR HALOGEN
MATERIALS IN THE CABINET.

6.3.3 Materials & Equipment

The apparatus and materials should next be placed into the cabinet. Care must be
exercised that no items be placed over the front intake grills. Materials should be
arranged so that clean, dirty (used), and virus materials are well separated. Passage of
contaminated materials over uninoculated cultures or clean glassware should be
avoided and transfer of viable materials should be performed as deeply into the
cabinet (away from open face) as possible.

6.3.4 Air Purge

Additional purging of the work space without user activity should be allowed for 2-3
minutes after materials and apparatus have been placed in it. This will rid the area of
all "loose" contamination that may have been introduced with the items.

OM0140 19
 6.3.5 Perform Work
The work can now be performed. The technician performing the work is encouraged
to wear a long-sleeved gown with knit cuffs and rubber gloves. This will minimize
the shedding of skin flora into the work area and concurrently protect the hands and
arms from viable agent contamination. At a minimum, the hands and arms should be
washed well with germicidal soap before and after work in the cabinet. For the
preparation of antineoplastic drugs, the following procedures summarize those
contained in OSHA Instruction PUB 8-1.1, "Work Practice Guidelines for Personnel
Dealing with Cytotoxic (Antineoplastic) Drugs." The above document should be
thoroughly studied/reviewed prior to drug preparation in the cabinet.

a. A sterile plastic-backed absorbent drape should be placed on the work surface

during mixing procedures. The drape should be exchanged whenever
significant spillage occurs, or at the end of each production sequence.

b. Vials should be vented with a filter needle to eliminate internal pressure or

vacuum.

c. Before opening ampules, care should be taken to insure that no liquid remains
in the tip of the ampule. A sterile gauze sponge should be wrapped around the
neck of the ampule while opening.

d. Final drug measurement should be performed prior to removing the needle

from the stopper of the vial.

e. A non-splash collection vessel should be available in the biological safety

cabinet to discard excess drug solutions.

6.3.6 Terminal Purging & Wipedown

Following completion of work, allow the cabinet to run for 2-3 minute period without
personnel activity to purge the unit. The decontamination of the interior surfaces
should be repeated after removal of all materials, cultures, apparatus, etc. A careful
check of grills and diffuser grids should be made for spilled or splashed nutrients
which may support fungus growth and resulting spore liberation that contaminates the
protected work environment.

6.3.7 Paper Catch

A permanent paper catch is installed behind the rear divider panel of the work zone.
This area forms the return air path to the motor/blower; and if the air flow is blocked,
it could seriously affect the performance of the cabinet. Therefore, THE PAPER
CATCH SHOULD BE CHECKED AND CLEANED NO LESS THAN A
WEEKLY BASIS; DAILY basis if procedures dictate the use of paper products.
Any paper removed must be properly disposed of as Contaminated Hazardous Waste.
6.3.8 Shut Down
Turn off blowers and lights. Do not use cabinet as a depository for excess lab
equipment during periods of non-operation. If antineoplastic agents are being
prepared in the cabinet, it is recommended to let the cabinet run 24 hours per day.
This lessens the possibility that contaminants may escape.

OM0140 20
6.4 Ergonomics
Ergonomics, the study or accommodation of work practices is extremely important for proper
cabinet usage and user health and safety. An evaluation of normal work practices should be
performed with each user when working in a cabinet. Evaluation criteria should be at a minimum:
a. Proper user posture
b. Effective workzone layout for work practice
c. Vision or sightlines

For each of the above evaluation criterion, several aids may be supplied to accommodate the user.
• Ergonomic chair - A six-way articulating seat and back control for personalized adjustment to
assure proper user posture. Be sure feet are resting on the floor, chair foot support or foot rest.
Also be sure back is fully supported with proper chair adjustments.
• Forearm/armrest support - The cabinet is provided with a forearm support on the work access
opening. Periodic mini-breaks during work practice should be taken resting forearm to avoid
stress and fatigue.
• Effective workzone layout - Always prepare your work procedure to minimize reach to avoid
neck and shoulder stress and fatigue. Rotating tables are optional to maximum workzone and
minimize reach.
Vision and sightline - Always prepare your work procedure to eliminate glare and bright
reflections on the window. Keep your window clean and sightlines clear to your effect workzone.

6.5 Cleaning Procedures

Cleaning the cabinet is an important function in terms of both containment and sterility. Use
the following procedure to effectively clean or surface disinfect the cabinet workzone surfaces.

a. Raise the sliding window to a full-open position, if desired.

b. Press the audible alarm silence or cleaning key on the front control panel to
silence the audible alarm during the cleaning process.
c. Apply appropriate disinfecting solution to cabinet surfaces. Most surface disinfectants
require a specific contact time, depending upon the microbiological agents used within
the cabinet. CONSULT APPROPRIATE DISINFECTANT DOCUMENTATION
FOR PROPER APPLICATION AND SAFETY PRECAUTIONS.
NOTE:DISINFECTANTS THAT USE CHLORIDES AND HALOGENS WILL
CAUSE DAMAGE TO THE STAINLESS STEEL SURFACES IF LEFT
ON FOR LONG PERIODS OF TIME.

d. After the specified contact time, wipe up excess disinfectant. IF THE

DISINFECTANT USED CONTAINS CHLORIDES OR HALOGENS, RE-WIPE
ALL SURFACES WITH 70% ALCOHOL OR SIMILAR NON-CORROSIVE
ANTI-MICROBIAL AGENT TO PREVENT DAMAGE TO STAINLESS
STEEL SURFACES.

6.6 Antineoplastic Decontamination Procedures

This procedure should be executed following a spillage and/or periodic maintenance, testing or
relocation of the cabinet. In addition, if the cabinet is being relocated or turned off for an
extended period, the work access opening and exhaust HEPA filter opening should be sealed
with plastic.

OM0140 21
 6.4.1 Preparation
Prior to beginning decontamination activity, personnel should put on a Tyvek1
isolation gown, 2 pair of vinyl gloves and a full faced HEPA filtered respirator. All
protective garments should be contained in 4 mil plastic bags and labeled for disposal
as chemotherapy waste after completion of the procedure. For the purpose of this
procedure, the term CLEANING is defined as the operation of wiping down with a
cloth wetted with a clean hot (above 60°C) detergent solution, followed by wiping
down repeatedly with sterile water to rinse. All cloths shall be contained in 4 mil
plastic bags and labeled for disposal as chemotherapy waste.

6.4.2 Procedure
a. Make sure that the cabinet remains in operational mode with internal blower
on.

b. Open the hinged or sliding view screen and secure in the full open position.

CAUTION: With the view screen in the full open position, personnel
protection is compromised and a full faced HEPA filtered
respirator must be worn.

c. Clean all readily accessible surfaces of the cabinet.

d. Remove perforated metal diffuser screen from the underside of the supply
HEPA filter and place on the cabinet work tray.

Note: Depending on the model, the diffuser screen is secured to the cabinet by #8-32
screws or 1/4" - 20 acorn nuts, 3 places. It is purposely a tight fit and is
secured to the back wall with projecting threadless studs.

e. Clean both sides of the perforated metal diffuser screen and remove it from
the cabinet.

f. Lift the cabinet work tray, clean both sides and remove it from the cabinet.

g. Remove the front perforated grill, place on the cabinet floor and clean
both sides. Remove from cabinet.

h. Clean work tray supports.

i. Working from top to bottom, clean all inside surfaces of the cabinet.
Take care not to wet the HEPA filter. If liquid has collected in the
plenum drain, aspirate it using an IV tubing into an evacuated container.
Label the evacuated container for disposal as chemotherapy waste.

j. Clean the plenum drain area and wipe dry.

k. If the cabinet requires maintenance and/or replacement of the HEPA filters,

the operation should be halted at this point to allow trained personnel to
complete replacement of the HEPA and/or maintenance action required.
1 Available from Lab Safety Supply, Janesville, WI 53547-1368, or other laboratory, industrial, or hospital supply distributors

OM0140 22
 6.4.3 Assembly

a. Replace front (if removed) grill.

b. Replace the work tray and carefully tighten the thumb screws.

c. Replace perforated metal diffuser screen over the underside of the

supply HEPA filter.

d. Wipe down all exposed surfaces of the work area with 70% isopropyl alcohol.

e. Prepare for aseptic operation.

OM0140 23
7.0 General Maintenance
CAUTION: All maintenance actions on this equipment must be performed by a qualified
technician who is familiar with the proper maintenance procedures required for
this equipment. This includes both certification as well as repair.

7.1 Decontamination
No maintenance should be performed on the interior of the Labgard cabinet (area behind
access panels) unless the cabinet has been microbiologically decontaminated, is known to
biologically clean, or known to be chemically inert. Surface disinfection is performed as
specified in the cleaning procedures.

If microbiological decontamination is necessary, use the following procedure:

1. Remove screws at each side of the control center and allow the control center to rotate
up.

2. Remove left and right window farings via fasteners.

3. Place decontamination equipment inside the work area. Reference decontamination

procedure, per NSF Standard 49, Annex G, or EN12469:2000, Annex J, using the
following chart to calculate chemical requirements.

Cabinet Size -200

Cabinet 48 x 24 x 24-1/2 in.
Dimensions (1.22 x .61 x .62 m)
Cabinet Volume 16.3 cu. ft.
(.462 cu. m)

Note, the outlets in the work area are energized as long as the cabinet is plugged in
and switched on the front panel. Unplug the cabinet before decontamination
equipment is plugged into these outlets or run the decontamination power cords under
the front seal area.

4. Use duct tape and plastic to seal the front and exhaust area.

CAUTION: BE SURE CABINET IS TOTALLY SEALED TO

PREVENT ANY LABORATORY EXPOSURE TO
DECONTAMINATION GAS.

5. Perform decontamination procedure per NSF Standard 49, Annex G, or

EN12469:2000, Annex J.

OM0140 24
 If the cabinet has been used to prepare antineoplastic drugs, (chemotherapy), or other toxic
chemicals, decontamination of the cabinet cannot be accomplished by the above procedure. It is
recommended that the following protective measures be taken:

1. Gloves
Gloves must be worn. Care must be taken not to cut, puncture, or tear the gloves. No
one glove material is impervious to all CYTAs; disposable surgical or polyvinyl
chloride (PVC) gloves provide substantial but not complete protection. PVC gloves
probably are more protective than surgical gloves, but they are stiffer and less tactile.
Gloves should be discarded after each use. Gloves should be tucked into the cuffs of
the gown. Double gloving should be considered.

2. Face & Eye Protection

A disposable dust and mist respirator and either a plastic face shield (preferred) or
chemical splash goggles must be worn. The face shield or goggles should be wiped
clean with a suitable tissue and water after each use.

3. Gowns
A protective garment must be worn. The garment should be made of lint-free, low-
permeability fabric and must have a closed front, long sleeves, and elastic or knit
closed cuffs. Tyvek1 isolation gowns are one example of an acceptable garment. The
garment must be worn outside the work area. Disposable gowns are preferred over
reusable. Front-buttoned coats are not recommended.

4. Hair & Shoe Covers

Disposable hair and shoe covers should be worn.

5. Motion
Slow and deliberate motions are necessary when working in the interior of the cabinet,
in order to minimize the generation of particulates.

Please consult with NuAire, Inc. about any unique contamination problems.

Normally, no preventive maintenance is required on the interior of the cabinet (i.e., the area
behind the access panel containing the HEPA filters and motor (blower assembly). All required
adjustments in order to maintain proper cabinet airflows are external to the cabinet interior. The motor
is lubricated for life and is thermally protected with automatic reset.

1 Available from Lab Safety Supply, Janesville, WI 53547-1368, or other laboratory, industrial, or hospital supply distributors.

OM0140 25
7.2 Lamp Replacement, Fluorescent
The two (T8) fluorescent lamps are cool white, rapid start and placed external to the cabinet to
aid maintenance and minimize heat build-up within the cabinet. The life rating of the lamp is
9000 hours based on three hour burning cycles.

To replace a lamp, it is necessary to lift the control center up.

1. First, switch Cabinet Light Switch off.

2. Second, remove the screws at the side of the control center.

3. Rotate the control center up and hold in place by using the support rod located in
control center.

4. The lamp bulb is removed by displacing the lamp to one side against the compressible
lamp holder.

5. Reverse the procedure to reinstall the lamp assembly.

7.3 HEPA Filter/Motor Replacement (Drawing BCD-02411)

The HEPA Filters under normal usage and barring an accident (a puncture), do not need
replacement until the efflux velocity cannot be maintained or the access inflow velocity cannot
be maintained at 100 LFPM (.51 m/s) (min.). This may permit the average downflow velocity
to be as low as 65 LFPM (.32 m/s) as long as no point falls below 20% of the average
downflow velocity.

The HEPA Filters should not be replaced until the entire cabinet has been decontaminated or
known to be biologically "clean".

7.3.1 Procedure

CAUTION: Disconnect electrical power from the unit before attempting any
maintenance action.

Step 1: Remove the sliding window assembly. First rotate up the hinged Control Center.
(Hold up or remove the control center. Do not use support rod to hold in place).
Second, remove the stainless steel front window trim. Third, remove (4) 1/4-20 acorn
nuts and related hardware so that sliding window assembly may be lifted away from
cabinet.
Step 2: Remove the supply and exhaust filter cover.

CAUTION: Screws are used in lieu of acorn nuts, and lockwashers. The screws have
O-rings and should be replaced if damaged or badly deformed.

The interior of the cabinet is now fully exposed for replacement of the filters and/or
motor/blower.

OM0140 26
 Step 3: Filter Removal
It is not always necessary to replace both the supply and exhaust filters at the same
time. If during the course of certifications, the downflow always falls off while the
exhaust increases (i.e. greater than 100 LFPM), the supply filter is "loading" faster
than the exhaust filter, and only the supply filter may need replacement.

a. To remove the supply filter "A":

1. First, remove the HEPEX/choke tray band clamp between the supply
HEPEX and the exhaust filter choke tray. Separate the plenum from
the choke tray.

Note: Double sticky back gasket is used to hold the plenum to the
choke tray and should be replaced when reassembling.

2. Remove the 2 hold-down clips (1 each side) holding the filter to

the frame.

3. Remove the HEPEX pressure plenum from the blower assembly.

The HEPEX is clamped to the blower assembly via a clamp.

4. Carefully remove the supply filter and HEPEX. The HEPEX can be
folded neatly to seal the contaminated side of the HEPA. Direct
exposure should be avoided.

CAUTION: Dispose of spent HEPA filters properly. Avoid direct

contact to "dirty side" of the filters. Label toxic waste.

b. To remove the Exhaust HEPA:

1. Relax the exhaust filter seal loading mechanism by turning the four
threaded bolts counterclockwise until one can see a definite release of
the loading springs.

2. Pull the exhaust choke tray free and remove the filter. It is not
necessary to remove the tray, although it is free to move forward
several inches, if necessary, to free the HEPA filter.

OM0140 27
 Step 4: Filter Installation
When installing new filters, use only filters of the same rated flow and size as
originally installed. It is recommended that a new HEPEX/Supply filter be installed
since the HEPEX is factory installed to the filter. However, field installation kits are
available separately from the filter.

a. To install the supply filter, simply reverse the procedure outlines in Step 3a,
above.

Note: Be sure to open the choke plate fully before inserting the filter into the tray.
This will assist in adjusting the airflow.

b. To install the exhaust filter, grease the top and bottom gaskets of the filter
with silicone grease and carefully insert into the exhaust choke tray.

c. Position the filter frame within the outside walls of the exhaust opening on the
top of the hood. Tighten the spring loaded bolts, 4 places, depressing the
gasket material by 1/8 inch (3mm).

Step 5: Motor/Blower Assembly Removal

a. It is recommended that the motor/blower to be removed as a single unit. To

remove, disconnect electrical connections to the motor, remove the HEPEX
pressure plenum and unbolt the motor/blower assembly from the roof of the
cabinet (4 places). Always inspect the rubber isolation motor mounts and
replace those that are cracked or visibly show stress.

b. Replace the motor exactly as originally installed in the blower housing,

paying particular attention to the correct electrical connections (see Electrical
Schematic).

c. Re-install the new motor/blower assembly.

7.4 Sliding Window Replacement & Adjustment

The sliding window replacement is accomplished by removing the front decorative panel,
control center and window glide assemblies. The sliding window adjustment may be required
due to everyday use over the life of the cabinet. The window glides can be adjusted by
loosening the frame fasteners and moving them closer or farther apart. When adjusting the
sliding window, be sure to verify proper microswitch operation. If the sliding window is too
loose, the sliding window will not properly activate the microswitches, thus causing potential
operational malfunctions to occur.

OM0140 28
7.5 Airflow Calibration
The NU-425-200 Airflow Calibration consists of adjustments to balance the airflow within the
cabinet. THIS WORK SHOULD BE DONE ONLY BY A QUALIFIED TECHNICIAN
WHO CAN MEASURE THE AIRFLOW FROM THE FILTERS WITH A SUITABLE
VELOMETER. NuAire provides two adjustments to balance the airflow within the cabinet.
These are:

a. Blower speed adjustment via motor voltage regulator

b. Exhaust filter choke

The blower speed control system adjusts the cabinet's total volume of airflow while the choke
adjusts or balances the exhaust airflow, as well as makes up for filter resistance tolerances.
Since it has been NuAire's experience that the filters may not "load" evenly, both adjustments
are necessary for proper cabinet performance.

The cabinet is considered to be certifiable if the following airflow measurements are present:

a. Downflow average: 70 LFPM + 5 LFPM (.35 m/s + .025 m/s).

b. Inflow average: 105 LFPM + LFPM (.53 m/s + .025 m/s) using the calculated
inflow velocity measurement method.

BEFORE STARTING AIRFLOW CALIBRATION PROCEDURE, LET THE

CABINET RUN FOR AT LEAST 10 MINUTES.

7.5.1 Downflow Calibration

Step 1: Place a velometer in the cabinet workzone on the horizontal plane 4 inches
(100mm) above the bottom edge of the viewing window. Spot check several
points on the recommended downflow velocity test grid found in Table 7.0.

Step 2: If necessary, adjust airflow control potentiometer, located under the

removable cap plug on front panel, to the above stated airflow requirements.

Step 3: Proceed to inflow calibration.

7.5.2 Inflow Calibration

Step 1: yMeasure the inflow velocity using the recommended procedure found in
Table 7.0. If necessary, adjust the exhaust filter choke, located under the front
decorative panel, to achieve the correct average inflow velocity within the
stated range of 105 + 5 LFPM (.53 + .025 m/s).

yLess than 110 LFPM (.51 m/s):

First, open the choke plate or make sure it is open. If this is insufficient, then
increase the motor speed control.

yGreater than 100 LFPM (.56 m/s):

First, adjust the motor speed control to achieve 1/2 the exhaust excess, then
close the choke plate to achieve the balance. In this fashion, the downflow
should remain nearly constant (i.e. what the reduced speed took away, the
choke plate restores).

OM0140 29
 Note: The choke plate adjustment requires a standard blade screwdriver. While
monitoring the exhaust flow to check position, turning the set screw clockwise
will open the choke while turning the set screw counter clockwise closes the
choke.

Step 2: Once exhaust adjustment is complete, return the downflow calibration and
then check average downflow velocity. If the downflow average remains
within the correct range, the calibration is complete. If not, readjust as
necessary to obtain the correct calibration range.

7.6 Filter Integrity Check

In order to check filter and filter seal integrity, the HEPA filter media and seals must be
directly accessible by the measuring instrument.

The diffuser plate placed below the HEPA to protect the filter during normal usage may be
removed as follows: The diffuser is secured to the cabinet shell by #1/4-20 acorn nuts located
immediately behind the front viewing window. After removing the fasteners, drop the front of
the diffuser plate several inches and pull forward gently. Note, that the diffuser is purposely a
tight fit - it is secured to the back wall of the cabinet interior by a light push - fit with
projecting studs.

The exhaust filter is typically more difficult to check, since charcoal filters, or exhaust
transitions could cover the filter. Access panels are usually provided and should be removed.
If an air gap exhaust transition is provided, the air gap must be sealed with duct tape or other
suitable means to prevent contaminated air from migrating into the exhaust efflux. All exhaust
blowers/fans should be turned off during the check.

7.7 Cleanliness Classification Test For Pharmacy Application

If this cabinet is going to be used within pharmacy, per USP797, the cabinet must be tested to
assure compliance to ISO 14644-1, Cleanrooms and Associated Controlled Environments, Part
1: of the Air Cleanliness. The cleanliness classification test is performed using a particle
counter to measure particle counts within the cabinet workzone. Turn on cabinet and let warm
up for several minutes. Turn on particle counter and flush out sample tubing line to remove
latent particles. Set the particle counter to measure 0.5 micron or larger particles at the
appropriate measuring rate.

Take 5 test points in 1-minute intervals on a grid, in a horizontal plane as measured

approximately 6-inches above the worksurface. The grid location is designed as the workzone
centerpoint and each corner measured 6-inches (152mm) from the inside perimeter.

Record the 5 particle count values for each of the test points over the 1-minute sample time.
All final count particle concentrations and calculated 95% upper confidence limit shall not
exceed 100 particles per cubic feet (ppcf) or 3520 particles per cubic meter (ppcm).

OM0140 30
 Table 7.0
Recommended Measurement Methods for Cabinet Downflow & Inflow

A. Downflow Measurement
a. Instruments: Alnor 8500 or TSI 8355 Thermoanemometer.
b. Procedure: Supply filter efflux is measured on a grid, in a horizontal plane 4 inches
(100mm) above the bottom edge of the window. No readings should be taken closer
than 6 inches (152mm) from the inside perimeter.
c. Test Data - Inches (mm):
200 6 12 18
(152) (305) (457)
6
(152)
12
(305)

Number of Readings: Average Velocity ft./min.(m/s)

d. Acceptance Criteria:
1. Average downflow velocity = 65 to 75 fpm (.33 to .38 m/s)
2. Individual readings must be within +20 percent of the average downflow
velocity. _____ to _____ fpm ( _____ to _____ m/s)
e. Meets Acceptance Criteria: Yes ____ No____

B. Inflow Measurement
a. Recommended Instrument: Shortridge Flowhood ADM-870 or Alnor 8500 or TSI
8355 Thermoanemometer.
b. Primary Procedure:
The primary procedure to determine inflow velocity uses a Direct Inflow
Measurement (DIM) Instrument (i.e. shortridge flowhood). The DIM Instrument can
be used directly on the cabinet with NO CORRECTION FACTORS REQUIRED if
operated in the local density default mode. The DIM Instrument should be equipped
with a flowhood that is as close as possible to the width of the cabinet. The DIM
Instrument should also be duct taped to the cabinet to prevent any sneak air paths from
occurring.

The DIM Instrument will read inflow volume (i.e. CFM). Use the window access
opening (1.36 ft2 /.126 m2 ) area to calculate inflow velocity.

OM0140 31
 Alternate Procedure:
The alternative procedure to determine inflow velocity uses a thermoanemometer in a
constricted window access opening of 3 inches (76mm) with the armrest removed. Inflow air
velocity is measured in the center of the constricted opening 1-1/2 inches (38mm) above the
work access opening on the following specified grid. Use the correction factor table to
calculate the inflow velocity.

c. Test Data - Inches (mm):

1. Dim Measurement
Inflow Volume ft.3/min.(m3/s) Access Opening ft.2(m2) Inflow Velocity ft./min(m/s)
2 2
(1.36 ft. /.126 m )

d. Constricted 3 inch (76mm) high access opening measurement - Inches (mm):

200 4 8.125 12.250 16.375 20.500

(102) (206) (311) (416) (521)

Number of Readings: Average Velocity of Constricted Area ft./min.(ms)

Average Velocity fpm X Constricted Area ft2 = Constricted CFM

of Constricted Area .51 ft2 (.047 m2 ) Area Volume

Constricted Area Volume CFM ÷ 8" ft2= Average Velocity fpm

Access Window Area of 8" Access
1.36 ft2 (.126 m2 ) Window Area

Average Velocity of 8" fpm X Correction Factor =Average Inflow Velocity fpm
Access Window Area .97

d. Acceptance Criteria:
1. Access Opening Inflow Velocity = 100 to 110 fpm (.51 to .56 m/s)
e. Meets Acceptance Criteria: Yes ______ No ______

OM0140 32
8.0 Error Indicators & Troubleshooting
Audible alarms and error indicators occur for a variety of reasons. Whenever an alarm
condition is present, the audible alarm and error indicator will be presented and stay on until the error
is cleared. When presented with an error indicator, please perform the following:

Step 1: NOTE ALL ERROR INDICATORS. When the cabinet is running, any and all red indicators
display an error.

Step 2: VERIFY ERROR INDICATORS. Error indicators can be verified by turning the errored
function on/off.

Step 3: MONITOR RE-OCCURRENCE OF ERROR INDICATORS. If re-occurrence of the error

indicator is immediate or daily, use guide below to correct the situation.

Error Indicator Troubleshooting Guide

Error Indicator Indicator Correction

Window alarm Sliding window is above Verify standard working

its standard working height and window
height or microswitch is microswitch operation.
not operating properly.

Cabinet fluorescent lights won't turn Check blower/light circuit

on. breaker on top of control
center. Check fluorescent
lamps. Check voltage to
light ballasts. Check
ballast. Check light
switch.
Cabinet blower won't turn on. Check sliding window for
correct operational height.
Check blower/light circuit
breaker on top of control
center. Check voltage to
blower. At motor voltage
regulator and at bulkhead
connector. Check wiring to
blower. Check blower
capacitor. Check sliding
window blower cutoff
microswitch. Check blower
motor. (Note: blower
motor has internal thermal
protector. Let blower
motor cool off for a
minimum of 30 minutes to
assure thermal protector is
not open.)

OM0140 33
Error Indicator Indicator Correction

Cabinet outlets won't turn on. Check outlet circuit

breaker on top of control
center. Check voltage to
outlets.

Cabinet ultraviolet light won't turn Check blower/light circuit

on. breaker on top of control
center. Check ultraviolet
lamp. Check voltage to
ultraviolet ballasts. Check
ballast. Check light
switch.

Blower/lights circuit breaker Check for short on output

continues to trip after reset. of circuit breaker. Replace
circuit breaker. Isolate
output of circuit breaker by
disconnecting control
center connectors, light
circuit, motor voltage
regulator, etc. to isolate the
short.

Minihelic gauge Minihelic gauge reads Check minihelic gauge

"NO" or "LOW FLOW" operation. Check for
pinched tubing in control
center. Make sure airflow
is not too low.

OM0140 34
9.0 Remote Contacts

9.1 Fan Relay

The fan relay contacts are single pole normally open contact closure outputs which are
activated whenever the blower is turned on. The contact points are located on the blower
switch. Contact ratings are 250 VAC maximum at 2 Amps.

OM0140 35
10.0 Optional Equipment
10.1 Ultraviolet Lamp

10.1.1 Overview
The germicidal ultraviolet is primarily intended for the destruction of bacteria and other
microorganisms in the air or on directly exposed surfaces. Approximately 95% of the
ultraviolet radiations from germicidal tubes are in the 253.7 manometer region. This is a
region in the ultraviolet spectrum which is near the peak of germicidal effectiveness. The
exposure necessary to kill the bacteria is the product of time and intensity. High
intensities for a short period of time, or low intensities for a longer period are
fundamentally equal in lethal dosage on bacteria (disregarding the life cycle of bacteria).
The intensity of light falling on a given area is governed by the inverse law; that is the
killing intensity decreases as the distance increases from the tube.

The germicidal tube is placed in the cabinet to provide an average intensity of 100
microwatts per square centimeter (for a new tube) falling on a horizontal plane
defined by the bottom of the work surface. The minimum requirement per
paragraph 5.12 of NSF Standard 49 is 40 microwatts per square centimeter (ref. NSF
Std. #49, June, 1976).

Since ultraviolet rays will not penetrate ordinary glass, it is recommended that the sliding
window be closed while the ultraviolet light is on within the cabinet; or that personnel
leave the cabinet face area.

10.1.2 Operation
The operation of the ultraviolet lamp is accomplished by closing the sliding window and
pressing the UV switch located on the front panel.

10.1.3 Precaution
The rays from germicidal tubes may cause a painful but temporary irritation of the eyes
and reddening of the skin, if of sufficiently high intensity, or if exposure covers a
prolonged period of time. For this reason, one should avoid direct eye and skin exposure
to ultraviolet light. If exposure cannot be avoided, it is necessary for personnel to wear
eye goggles or face shields, and long sleeve gowns with rubber gloves.

Since ultraviolet rays will not penetrate ordinary glass, it is recommended that the sliding
window be closed while the ultraviolet light is on within the cabinet; or that personnel
leave the cabinet face area.

10.1.4 Maintenance
The output of an ultraviolet lamp deteriorates with burning age. The useful life of the
lamp is approximately 7000 hours under specific test conditions.

It is recommended that either a time schedule be established or the tube's output be

measured periodically and the tube replaced when its output falls below 40 microwatts per
square centimeter or exceeds 7000 hours of operation. Lamps should be allowed to
operate approximately 5 to 10 minutes (longer when the lamp is in low temperatures) to
warm up sufficiently and wiped clean of dust or dirt before reading the output with a
meter. Even minute amounts of dust will absorb ultraviolet energy. The lamp may be
cleaned with a lint-free cloth dampened with alcohol or ammonia and water.

OM0140 36
 Energies Required to Destroy Some Microorganisms By Ultraviolet Radiations(e)
Microwatt Microwatt
Mold Spores seconds Protozoa seconds
per cm/2 per cm/2
Penicillum roqueforti 26,400 Paramecium 200,000(a)
Penicillium expansum 22,000
Penicillium digitiatum 88,000 Nematode Eggs 40,000(b)
Aspergillus glaucus 88,000
Aspergillus flavus 99,000 Algae 22,000(c)
Aspergillus niger 330,000
Rhizopus nigricans 220,000 Virus
Mucor racemosus A 35,200 Baceriophage (E. Coli) 6,600
Mucor racemosus B 35,200 Tobacco Masaic 440,000
Oospora lactis 11,000 Influenze 3,400(d)
Yeasts
Saccharomyces 13,200
Ellipsoideus 17,600
Saccharomyces cerevisiae 13,200
Brewers' yeast 6,600
Baker's yeast 8,800
Common yeast cake 13,200
Bacteria
Streptococcus lactis 8,800
Strep. hermolyticus (alpha type) 5,500
Staphylococcus aureus 6,600
Staphylococcus albus 5,720
Micrococcus sphaeroides 15,400
Sarcina lutea 26,400
Pseudomonas fluorescens 7,040
Escherichia coli 7,040
Proteus vulgaris 7,480
Serratia marcescens 6,160
Bacillus subtilis 11,000
Bacillus subtilis spores 22,000
Spirillum rubrum 6,160

References:

(a) Luckiesh, Matthew (1946) Application of Germicidal, Ethyemal and Infrared Energy, D. Van
Nostrand o., New York, New York, pp 253
(b) Hollaender (1942) Aerobiology, A.A.A.S. (for 90% inactivation), pp 162
(c) Ellis, C. and Wells, O.O. (1941) The Chemical Action of Ultraviolet Rays, Reinhold
Publishing Corp., pp. 713-714
(d) Hollaender, A., Oliphant, J.W. (1944) The inactivation effect of monochromatic ultraviolet.
Radiation on Influenze Virus (for 90% inactivation) Jour. of Bact. 48, pp. 447-454
(e) This table, "Energies Required to Destroy Some Microorganisms by Ultraviolet Radiations"
comes from Westinghouse brochure entitled - "Westinghouse Sterilamp Germicidal
Ultraviolet Tubes"

OM0140 37
11.0 Electrical/Environmental Requirements

11.1 Electrical: * NU-425-200 115 VAC, 60 Hz, 1 Phase, 10Amps

**NU-425-200E 230 VAC, 50 Hz, 1 Phase, 5 Amps
NU-425-200G 220 VAC, 60 Hz, 1 Phase, 5 Amps
*UL Listed **CE Certified

11.2 Operational Performance (for indoor use only)

Environment Temperature Range: 60°F - 85°F (15.6°C - 30°C)
Environment Humidity: 20% - 60% Relative Humidity
Environment Altitude 6562 Feet (2000 Meters) Maximum

11.3 Light Exposure

Standard Fluorescent Lighting @ 150 ft. candles (1614 LUX) maximum intensity.

11.4 Installation Category: 2.0

Installation category (overvoltage category) defines the level of transient overvoltage, which
the instrument is designed to withstand safely. It depends on the nature of the electricity
supply and its overvoltage protection means. For example, in CAT II, which is the category
used for instruments in installations supplied from a supply comparable to public means, such
as hospital and research laboratories and most industrial laboratories, the expected transient
overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply.

11.5 Pollution Degree: 2.0

Pollution degree describes the amount of conductive pollution present in the operating
environment. Pollution degree 2 assumes that normally only non-conductive pollution such as
dust occurs with the exception of occasional conductivity caused by condensation.

11.6 Chemical Exposure

Chemical exposure should be limited to antibacterial materials used for cleaning and
disinfecting. CHLORINATED AND HALOGEN MATERIALS ARE NOT
RECOMMENDED FOR USE ON STAINLESS STEEL SURFACES. Chamber
decontamination can be accomplished by paraformaldehyde, vapor phased Hydrogen Peroxide
or Ethylene Oxide without degradation of cabinet materials.

11.7 EMC Performance (classified for light industrial)

Conducted Emissions: CISPR 11, Class B & EN55011
Radiated Emission: CISPR 11, Class B & EN55011
Radiated Immunity: EN50082-1, IEC 801-3, Level 2
ESD Immunity: EN50082-1, IEC 801-2, Level 2
EFT/BURST Immunity: EN5082-1, IEC 801-4, Level 2

(Note: The EMC performance requirements are generated within the product enclosure. The
enclosure will be all metal grounded to earth. In addition, the membrane front panel will also
include a ground plane for maximum protection and an electrostatic shield.

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