Biosafety cabinet user manual

1
Appendix

1 technical drawing

2-7 general information

8 -9 electricity

10-11 filters

12-16 sash changing

17-22 control system, calibration and airflow settings

23 maintaining the Hood

24 Troubleshooting

25 alarms + night mode

26-28 fan data

28-30 filters data

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3
 Getting Started

After the installation has been prepared, the Biosafety cabinet can be inspected, installed and tested.

The information below will guide you in performing the following actions:

• Unpacking and moving the Biosafety cabinet.

• Installing the hood.

• Connecting the electrical supply source.

• Connecting the service lines.

• Testing and certifying.

Note: The Biosafety cabinet weighs between 180-260 kg. The shipping pallet allows for lifting with a
mechanical lift truck or floor jack. If you must lift the cabinet manually, at least 2 people are required,
and guidelines for safety must be strictly followed.

Unpacking the Biosafety cabinet

Carefully remove the outer wrapping and inspect the hood for damage that could have occurred during
the transportation of the unit. If damage has taken place, please notify the delivery carrier immediately
and leave the entire box as is, intact for the carrier’s inspection.

Please do not return the product without the prior authorization of TopAir Systems. Unauthorized
returns will not be accepted.

If the cabinet was damaged during the transportation, please file a claim directly with the freight carrier.
TopAir Systems is not responsible for shipping damages.

Do not discard the package in which the cabinet is wrapped until all of the components have been
checked, installed and tested. The hood is secured to the pallet on both sides. To access the nuts and
bolts, remove the side panels by unscrewing the Philips screws on both panels (Keep the screws). Swing
the front of each panel away from the cabinet, and lift it straight up to remove the panel from the
cabinet.

The following articles are packed in a box located underneath the work surface:

- User manual
- Test report

If any of the components listed for the cabinet are not present, or in the case that any of the
components are damaged, contact TopAir Systems immediately for further instructions.

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 Moving the Biosafety cabinet

Moving the hood should be performed with care. You can move the hood while it’s attached to its
pallet, by using a floor jack, or a furniture dolly located underneath the unit. Do tilt the hood onto a
hand truck.

Installation of the Biosafety cabinet on an Existing Work Surface

Note: The Biosafety cabinet is heavy. Use caution when lifting or moving it.

During the installation of the Biosafety cabinet on an existing work surface or benchtop, please make
sure that the structure can safely support the weight of the hood along with the weight of any related
equipment. The work surface should be at least as wide as the Hood for proper support. A hole or notch
may be added in the supporting surface in the front right corner for the optional drain valve.

Installing the Hood on a TopAir Systems Base Stand

TopAir Systems offers a Base Stand in a variety of configurations to suit a variety of customer needs.

WARNING: Lift and support the weight of the cabinet only from the sides and back. Damage will occur if
the cabinet is lifted or supported from the front edge.

Move the base stand into its final location. Using a carpenter’s level, adjust each leveling foot until the
stand is level on both planes. At this point, the hood can be lifted on to the designated stand.

5
 Initial Certification

Before using the TopAir Systems Biosafety cabinet a qualified certifier must certify it. Under normal
operating conditions, the Biosafety cabinet should be recertified at least once a year. It should also be
recertified when it is relocated or serviced. The certifier should perform tests, in accordance with the
local market.

Biosafety cabinet Check and Certification

The test should include:

- Noise level test,

- Lightening intensity test
- down flow velocity test
- particle counting test
- D.O.P test

Sterilization
Before the equipments sterilization, do not remove the front grids and the drainage valve.
The equipment should be sterilized as follows:
• Before operation
• Before replacement of the filter
• Before performance testing (unless the equipment is new and has never been used)
• Before moving the equipment
• Prior to initiating a totally different work procedure
• Upon request of the safety office or safety committee
*These tests are user comfort related tests and may be omitted at the user’s or certifier’s discretion.

If you have any questions regarding certification agencies or need assistance in locating one, contact
TopAir Systems’s Product Service Department at +1-855-6-TOPAIR

Location Requirements

The cabinet must be in a location which is not in the proximity of doors, fans, ventilation registers, fume
hoods and air-handling units that could cause damage to its airflow patterns. The windows in the room
should be shut. The drawing below provides guidelines for the preferred location assigned to the
Biosafety cabinet.

Environment limits
41° to 104°F / 5°C to 40°C
Max RH: 80% for temp up to 88°F / 31°C

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Electricity Specifications

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Various models of the Biosafety cabinet models have these electrical requirements.

Model # Requirements
BO-90 115 VAC, 60 Hz, 4 Amps
BO-90 230 VAC, 50/60 Hz, 2 Amps
BO-120 115 VAC, 60 Hz, 4 Amps
BO-120 230 VAC, 50/60 Hz, 2 Amps
BO-150 115 VAC, 60 Hz, 6 Amps
BO-150 230 VAC, 50/60 Hz, 3 Amps
BO-180 115 VAC, 60 Hz, 4 Amps
BO-180 230 VAC, 50/60 Hz, 2 Amps

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HEPA filter

Product Overview

 Tested to ensure certified performance

 Reduces operation costs with lowest possible pressure drop from microglass media
 Gasket seal design
 Optional media ribbon or hot melt separators
 Choice of efficiencies
 Lightweight, compact

Specifications:

 Efficiency: HEPA ULPA

 Media type: Fiberglass
 Frame material – aluminum
 Separator style – hot melt
 Special size available – yes
 Antimicrobial available – n9o
 Max operation temp. 66 degrees Celsius
 Filter depth – several options
 Air filter specification: UL900

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Filter Replacement
lower filter

upper filter - same method, roof location.

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Front glass replacement:

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16
control system

Electric Socket - Click this button to activate the electric socket of the unit.

On/Off – Click this button to shut the unit on and off. It is recommended to switch on the unit five or ten
minutes prior to any activity.

LED light – Click this button to operate the fluorescent lights. When the sash is fully closed, the fluorescent lights
automatically shut off.

UV light – Click this button to operate the UV (germicidal) light. When the sash is fully closed, the UV light can be
programmed to automatically turn on. When the sash is raised, the light automatically turns off, to prevent
exposure to UV radiation.

Fan Button – Click this button to turns the fan on and off.

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 settings:

time setting

timers settings

18
test remainder

Distributor DATA uploading:

on calibration screen (pass word is 4096), add the distributor data.

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 calibration
1 Go to calibration screen, threw settings, press the password

A. locate a calibrated air velocity vane, 12” / 30cm above the working surface, in the middle.

B. use the up/down arrows until you will get air velocity of 0.33 m/s 66fpm on the vane -
press "save"
Go with the right arrow to the next screen.

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3

A. use the up/down arrows until you will get air velocity of 0.25 m/s 50 fpm on the vane -
press "save"

The airflow specifications of TopAir systems BO-120-PP are:

Down flow, 12” above the working surface, 50-68 FPM.

Inflow average when sash id 4” open, 180-210 FPM.

Inflow air volume - 220-240 CFM.

__________________________________________________________

The airflow specifications of TopAir systems BO-150-PP are:

Down flow, 12” above the working surface, 50-68 FPM.

Inflow average when sash id 4” open, 180-210 FPM.

Inflow air volume - 285-310 CFM.

___________________________________________________________

The airflow specifications of TopAir systems BO-180-PP are:

Down flow, 12” above the working surface, 50-68 FPM.

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Inflow average when sash id 4” open, 180-210 FPM.

Inflow air volume - : 220-240 CFM.

Screen cleaning mode

Press the "hand " icon, the touch screen will freeze and allow cleaning.

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Maintaining the Hood
The service operations required for maintenance of the Biosafety cabinet
to ensure top level performance are listed below.

Routine Maintenance Schedule

Weekly
• Using 70% ethanol, or another disinfectant, disinfect the surface of the hood interior and the work surface.
• Operate the hood blower, and record the remaining filter life % in an operational log.

Monthly

• Using a damp cloth, clean the exterior surfaces of the hood, especially the front and top of the cabinet, to
remove dust.
• Disinfect and lift the work surface. Disinfect the surface of the lower plenum with a solution of 70% ethanol or
another disinfectant.
• Check all service valves for proper operation.

Semiannually or Annually
• Have the cabinet re-certified by a qualified certification technician.

Storage
If the cabinet is not used for over a month, it should be placed in storage conditions..
Note:
- The hood should not be stored in areas of excess humidity or extreme temperatures.
- If the hood is moved during storage, it must be recertified before use.
1. Close the sash to the end and seal the bottom edge and the exhaust outlet with plastic sheets.
2. Unplug the cabinet.
3. Make sure that the hood is not moved or disturbed during storage.

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 Troubleshooting

PROBLEM CAUSE CORRECTIVE ACTION

Cabinet blower and Plug the hood into the appropriate electrical
lights won’t turn outlet.
on Unit not plugged into outlet
Check connection to control box on the top
of the hood.
Circuit breaker(s) tripped/fuses Reset circuit breakers/replace fuse
burnt out

Keypad disconnected
or defective Check connections

Inspect blower wiring

Blower won’t turn Blower wiring is disconnected
on but lights work

Replace blower motor

Blower motor is defective

Keypad disconnected or Check connection or replace keypad

defective

Check the lamp fuse on the PCB

LED light not Defective lamp or driver Replace lamp or driver

working
Lamp wiring is disconnected Inspect lamp wiring
Airflow problem Filter(s) are blocked Replace filter(s)
Fan speed is low Recalibrate the unit
Fan is damaged Replace fan
Check the limit micro switches on the inner tight
side post/ check the sash motor/ check the fuse
Sash problem Sash doesn’t move on PCB
Check the time setting in timers screen
Check the micro switch on the right sash track
Uv problem UV doesn't work Replace uv bulb

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 alarms

Type of alarm Action need to be taken

Sash position is Use the down arrow and make sure the
too high sash is on the height label line

Periodic test Make sure that a professional technician

test the product and provide certifications.
set the new date for periodic test on the
timers screen.

Replace UV light Replace UV bulb

Replace filters Make sure the unit can pass all the tests,
else - replace filters

Low air velocity Stop all actions in the cabinet until the
problem solved

Night mode

By activating the night mode on settings screen, all the cabinet's element will be shut down
immediately after UV cycle will be finished,
By pressing the wakeup button, the cabinet returns to normal activity

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EC fan data

1-1 Rated Voltage 230VAC

1-2 Frequency 50/60Hz

1-3 Operating Voltage 200~277 VAC

1-4 Low-Start Voltage ≤199VAC

1-5 Rated Speed 1930RPM

1-6 Input Current 2.02A

1-7 Input Power 467W

1-8 Air Flow 2190CFM /3720m3/H

1-9 Static Pressure 2.96inH2O / 740Pa

1-10 Acoustical Noise 73dB(A)

1-11 Life Expectance 50,000Hours(L10)

At 40℃ room, humidity 15%~65%RH.

1-12 Direction of Rotation Anti-clockwise

seen on motor

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U15 ULPA filters

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