Agilent LC and Empower

Service Training

©2008 Waters Corporation

SQT &MP for Agilent LC
Class Objective:

By the end of class you will be able to:

o Install and configure Agilent Ethernet Devices into Empower

o Operate Agilent LC’s using Empower software
o Complete the Agilent IQ protocol in Empower Node
properties
o Setup the Test Injections for Agilent LC’s in Empower
o Complete all developed MP protocols on Agilent systems
supported by Agilent SQT for Empower
o Complete the Agilent SQT protocols

© 2008 Waters Corporation 2

General Class Agenda

Day 1 Day 3
ƒ General Overview ƒ Agilent SystemsQT
ƒ Agilent Install / Configuration ƒ SystemsQT Operation
ƒ Operation / Instant Pilot ƒ Instrument Method Creation
ƒ Test Injections ƒ Running SystemsQT
ƒ Agilent Website ƒ Specialty products (1200SL)

Day 2 Day 4
ƒ Maintenance Protocols ƒ SystemsQT Results
ƒ Pumps Protocols ƒ Failure/retests
ƒ Standard Injector Protocols
ƒ Lab Cleanup
ƒ Well Plate Injector Protocols
ƒ Test
ƒ VWD/MWD/DAD Detector Protocols
ƒ Troubleshooting – Optional ƒ Review of Test
Maintenance ƒ Wrapup

© 2008 Waters Corporation 3

Required Components for Agilent
SystemsQT

ƒ Agilent 1100/1200/1200SL System with required Firmware

ƒ Empower 1154 1254 with Appropriate Service packs
ƒ Waters Agilent DAD Key Disk
ƒ Waters “Agilent Maintenance Protocol” parts kit for Agilent
Pumps Injectors and Detectors
ƒ SystemsQT Key Disk
ƒ Waters Agilent SystemsQT Chemical kit, Protocol DVD, and
Projects
ƒ Waters Xbridge 4.6mm x50
ƒ Waters Service Representative Certified in FSE Agilent :
SystemsQT and MP for Agilent LC”

© 2008 Waters Corporation 4

Class Materials for Systems QT
for Agilent LC

ƒ Materials on DVD
— Agilent Firmware
— Agilent marketing Info – current
sampling of Agilent service marketing
documents from the knowledge center
— Agilent Class Materials for Course
o SQT and MP for Agilent LC Student
Package
o MP Protocols for Agilent LC
o Operators manuals you Downloaded
from Agilent website
— Agilent SQT Projects and protocols

© 2008 Waters Corporation 5

SQT and MP for Agilent LC
Package

ƒ Goals and Objectives – Schedule

ƒ Lab Exercises for Course
ƒ Procedures for Configuring an Agilent
System into Empower
ƒ MP for Agilent LC’s
ƒ Forms and Documents for SQT and MP
for Agilent LC and MP
ƒ SQT procedures for Agilent LC
ƒ Exam for Agilent LC
ƒ Process for writing up field service
report
ƒ Agilent SystemsQT Part Numbers
ƒ Ethernet Configuration Guide for Agilent

© 2008 Waters Corporation 6

Empower – Agilent Installation Requirements
A1100/1200/1200 SL

ƒ Empower 1154
— Service Pack L
— Agilent A1100 1.05.1094 ICS Control pack or higher for
Empower
— Agilent Firmware A.06.04

ƒ Empower 2154
— FR 3, FR4 , Service Pack D or above*
— Agilent LC ICS 1.05.1094 ICS Control pack or higher for
Empower
— Agilent Firmware A.06.04

*SP D is not required – FR5 will work when released

© 2008 Waters Corporation 7

Agilent A1100 1.05.1094 ICS Control
Pack for Empower

ƒ Empower 2154 - 1154

— ICS can be installed during the initial setup, or post install
— ICS should be installed on all clients and/or Citrix servers that
acquires from or reads Agilent methods
— Installed ICS components are listed in “Add Remove Programs”
— Remove ICS components using “Add Remove Programs”

© 2008 Waters Corporation 8

Agilent System Configurations that
are supported with Empower

ƒ Empower 1154/2154 Control

— Most 1100/1200/1200 SL configurations
— Some Agilent/Waters mixed control (Limited support)
— 1200SL configuration supported*
— 1200Sl RRLC (Rapid Resolution LC) as a Method
— No TQ SQ 3100 with Agilent Systems*
— No VWD+ or ELSD support

ƒ Agilent SystemsQT Support

— Only full Agilent Series system supported
— No mixing of Waters components
— 1100/1200/1200Sl Configurations
— Column switching Valves

© 2008 Waters Corporation 9

Agilent Supported
1100/1200/1200SL Configurations

Pumps Injectors Detectors Heater/Column Valves

© 2008 Waters Corporation 10

Empower Control of Agilent Systems:
Summary of Supported Components

1100/1200 1200SL
Degasser G1322B - G1379A G1322B - G1379B

Pumps Isocratic - G1310A Binary - G1312B SL

Binary - G1312A
Quat. - G1311A
Autosamplers Standard - G1329A - G1329B Standard - G1329C SL
Well Plate - G1367A- G1367B Well Plate - G1367C SL
UV VWD G1314A – G1314B VWD G1314C SL
Detectors DAD G1315 –G1315B - G1315D DAD G1315C SL
MWD G1365A – G1365B – G1365D MWD G1365C SL
RI & Fluorescence RI G1362A*
Detectors FLD G1321A*

Column G1316A G1316B

Compartment Switching Valve

Handheld Controller G1323B G4208A Instant Pilot

* Not currently in SystemsQT

© 2008 Waters Corporation 11

Agilent Non Supported Functionality
with Empower

ƒ 1200 MSD, 6400, and 6410 Mass Detectors

ƒ Early Maintenance Functionality (EMF)

ƒ A1100/1200/1200 SL Maintenance DVD (Not Permitted!)

ƒ Diagnostic/Maintenance Tests (Use Instant Pilot)

ƒ Solvent Bottle Fillings

ƒ Electronic Column Identification

ƒ ELSD & VWDSL (new 160 hz)

© 2008 Waters Corporation 12
Empower Control for SystemsQT:
Agilent Firmware Requirements

ƒ Empower requires Agilent Firmware to be at A.06…. Or Higher

ƒ Currently:
— Customer/Agilent Reps Responsibility
— Agilent web site has Firmware Utility program
— Access to program requires user registration
— http://www.chem.agilent.com/scripts/cag_firmware.asp#pg8

ƒ Future:
— Customer will get Firmware from Agilent website
— Waters FSE upgrades new Agilent firmware updates

© 2008 Waters Corporation 13

Agilent 1100/1200 Hand-Held
Controllers G1323B & Instant Pilot

G1323B Instant Pilot

Not Supported with This is the only device

Agilent Systems QT supported with Agilent
protocols. Systems QT protocols.

© 2008 Waters Corporation 14

Agilent Instant Pilot

ƒ Verifies A1100/1200/1200 SL module firmware levels

ƒ Used for setting static IP addresses on 1100/1200 for Empower

system configuration

ƒ Instant Pilot control is disabled during Empower acquisition

ƒ Use for Waters Maintenance Protocols and Diagnostic Tests

ƒ Input of EMF (Early Maintenance Feature) and Bottle Filling -

not supported in Empower

ƒ Print / Screen Capture of Instant Pilot screens.

© 2008 Waters Corporation 15
Agilent Control & Option Disks

Requirements:
ƒ <=4 Agilent Systems may be configured with a
Lace/workstation

ƒ Two systems max if Diode Array

ƒ Option is installed once on the server

ƒ Paper licenses required for multiple Agilent systems

© 2008 Waters Corporation 16

Hardware Connections: Agilent Module
Interconnections

ƒ Connect CAN cables to each Agilent module’s CAN port

ƒ Do not confuse CAN Connections with Ethernet Connect
ƒ Connect Instant Pilot cable to extra CAN port

CAN Cable

© 2008 Waters Corporation 17

Hardware Connections: Agilent System
Connections to Empower Computers

Computer: Agilent Connections:

ƒ Connect Ethernet to Water’s ƒ Direct to Computer - Crossover

Instrument LAN Card Ethernet cable (Red)
ƒ Switch box - Standard Ethernet
cable (Yellow/Blue)

© 2008 Waters Corporation 18

Hardware Connections: Agilent
Connections to Waters Satin A/D’s

Needed (must be ordered separately from Agilent)

o Analog Cable: HP1200 to general purpose: 01046-60201

o Cable Universal Remote: HP1100 to general purpose

(to trigger SATIN) 5061-3378

o Cable Remote HP1100 to HP1100 (triggers unsupported

detectors from controlled Agilent modules)

© 2008 Waters Corporation 19

Software Configuration: Agilent
Systems in Empower
See page 7 of “SQT and MP for Agilent LC Student
Manual” for Software Configuration Procedure

© 2008 Waters Corporation 20

Agilent Systems in Empower:
Ethernet Troubleshooting
ƒ Waters Instrument LAN Troubleshooting:

— Use different LAN segment than customers network

— Use 3 three IP ranges:
o Default IP 192.168.0.x
o Alternate IP 172.16.0.x
o Customer IP - customer ensures subnet is correct

ƒ Agilent connection to Instrument LAN Troubleshooting

— Two different style cards:

o JetDirect (400N J4100A) - supports DHCP
o LAN Communication (G1369A) - no support of DHCP

— Connect LAN to highest data rate Agilent detector

© 2008 Waters Corporation 21

Agilent Systems in Empower:
Ethernet Troubleshooting
ƒ For multiple Agilent systems: Network switch p/n 725000455

ƒ No Support for Agilent Ethernet devices connected Empower with:

— network hubs
— wireless networks
— bridged network Ethernet cards

ƒ Other Waters DHCP connection cautions:

— No Connection of network printers
— Do not exceed 100 meters cable length
— Do not connect Ethernet instruments to a corporate network
o Not structurally validated
o LAC/E™32 module is unable to buffer instrument data.
o Network traffic can interfere with data transfer from instruments

© 2008 Waters Corporation 22

Empower Control of Agilent
Instruments

See Empower – Agilent Help File for full support Information

(ICS must be installed)

© 2008 Waters Corporation 23

Agilent Pump Assemblies Agilent Pump
Instrument Configuration

© 2008 Waters Corporation 24

 Agilent Pumps – Software Control

Pumps
ƒ 3 Styles Pumps- All Manual Purge Valve
— Binary 2 Pistons
o Mixer may be used or taken offline
o Standard – 400 Bar (~6000 psi)
o SL – 600 Bar (~9000 PSI)
— Quaternary – 1 Piston
o GPV Valve
— Isocratic – 1Piston
ƒ Standard on All
— Auto Stroke volume
— Auto Solvent Compressibility
— No Gradient Curves – Only linear
ƒ Options
— Seal Wash
— Solvent Select Valve – 1200SL Model

© 2008 Waters Corporation 25

Agilent Pumps – Software Control

Events: Open - Closed

No Pulsed
1 Function to Collect
and Monitor
Solvents Select Valve
(Binary)

1 Bar ~ 15 PSI

Seal Wash
(If present)
© 2008 Waters Corporation 26
Agilent Auto Injectors
Software Control

Injectors
ƒ 2 Style Injectors
— Standard
o Moves Vial to Injector
o 100 Vial Capacity
o Washes needle using additional vial (Bird bath)
o Optional Heater/Cooler
— Well Plate
o Standard 100 Vial or 2 Well Plates (96-384)
o Optional Heater/Cooler
o Needle Wash using Peristaltic Pump to Needle port
o Optional: Washes needle using additional Vial
o Load Ahead and Needle off-Line Functions

© 2008 Waters Corporation 27

Agilent Standard
Autoinjector Control with Heater

Events: Open – Closed

No Pulse

= 2.3 Standard

100 Standard
Operating Specs:
4 – 40 in 1°
increments

Pre- Loads the

next Sample
(Load Ahead)

Needle and Syringe Settings

© 2008 Waters Corporation 28

Agilent Well Plate
Autoinjector Control

= 2.3 Standard
100 Standard

Load Ahead –
Loop Off-Line

Use Wash
Needle in
Flush Port

Use Wash Needle in

Flush Port
Which
Tray for
Wash
Multiple Needle Wash
Cycles and Times

© 2008 Waters Corporation 29

Agilent Detector
Software Control
Detectors

ƒ 6 Detector Offerings
— VWD – Variable Wavelength Detector 190 - 600
o Standard – 10 hz
o VW SL (replaced by VW SL Plus) 80hz
o VW SL Plus – 180hz* - new yet supported
— DAD Diode Array Detector – MWD - Multi-
Wavelength Detector
o 190-800
o 1-16nm resolution
o Bandwidth 1-100nm
o Standard 5hz - SL – 80hz
o 3D & 2D Data DAD
o 2D only MWD
— *Fluorescence – Excitation, Emission, 3D
— *Refractive Index

*No SQT Support

© 2008 Waters Corporation 30

Detector Data Rates
Peak Width conversion to Pts/Second

© 2008 Waters Corporation 31

Agilent VWD Detector
Software Control

See on-line help for

procedure to do this
function.

© 2008 Waters Corporation 32

Agilent DAD Detector
Software Control
3.
1.

2.

Sample and Reference is like ACQUITY detectors

baseline correction (MBF).
1. Two Lamps:
• UV – Deuterium
• Visible – Tungsten
2. Slit = Resolution:
• Higher Slit – More Sensitivity
• Higher Slit – Less Linearity
3D Spectra
3. Negative Peaks -must be for
Margin selected to absorbance
negative
collect full 3D Data.

© 2008 Waters Corporation 33

A1100/1200/1200 SL System in Pro Run
Samples View

Control Panel for the 1100

system

• Module status are displayed

• Module status is shown in rolling
view
• Allows immediate update of
instrument settings
• Use for pump purge (manually
open)

© 2008 Waters Corporation 34

Message Center displays Agilent Systems
acquisition errors

Use for troubleshooting

© 2008 Waters Corporation 35

Changes in flow or
% composition traceability

ƒ Tracking flow rate or % composition with an Agilent system under

Empower control

— Requires Full Audit Trail projects

— Tracked in the Acquisition Log.
— Retains record of all changes made to methods, templates,
results, and samples for a project
—
ƒ Audit trail for injections is viewed in the Acquisition Log report
group

ƒ Audit trail comments for results are viewed by with a Result

Comments view filter.

ƒ Empower software preserves the Full Audit Trail Support with

backup and restoring projects.

© 2008 Waters Corporation 36

Red lights errors on
Agilent Modules

1. View Message Center to view

module and error
2. Use Instant Pilot to view error
log
3. Red lights on Modules:
ƒ Check for missing vials
ƒ Check Solvent leaks and leak
detectors
ƒ Restart using Run Sample’s
Agilent control panel
4. Status Lights
— Off = ready/pre-run
— Yellow= not ready
— Green = run mode
(performing an analysis)

© 2008 Waters Corporation 37

 Leak Detector Errors:
Check Column Fittings First

ALS into heating block

Block into Column

© 2008 Waters Corporation 38

Leak Detector in the TCM

Dry leak detector with Kim Wipe. Some use hair

dryer for difficult to reach areas
© 2008 Waters Corporation 39
What is Agilent SystemsQT?

ƒ Waters System Qualification Tool for Agilent Systems

ƒ SystemsQT is comprised of
— a simple wizard interface embedded into Empower 1154 and 2154
— System and Agilent Specific Project and Documentation DVD
— SystemsQT Option Disk.

ƒ SystemsQT option disk enables the SystemsQT option

— “Qualify System” is then available from the File menu in Run Samples
— The SystemsQT option disk is the property of Waters and is not left with the
customer

ƒ One SystemsQT option disk shipped with every order

ƒ Order an additional SystemsQT option disks - PN: 667003232

© 2008 Waters Corporation 40

Order of SystemsQT for Agilent LC

1. Survey Customer’s Instruments and Agilent

Modules
2. Agilent LC Installation and Configuration into Empower
ƒ Confirmation of firmware/modules/ICS
ƒ Additional connections
3. Test Injections
ƒ Confirm current state of operation
ƒ Confirm if new instrument methods need created
4. Maintenance protocols
ƒ Change parts according to the MP protocols
ƒ Inform customer of needed additional parts/charges
5. Test Injections
6. Run the SystemsQT tests
© 2008 Waters Corporation 41
Waters Maintenance Protocols on
Agilent 1100/1200/1200SL Systems

ƒ Checklist documented in Agilent SystemsQT Documentation

ƒ More details in Service Notes under Agilent SystemsQT*

ƒ See Service Course Training CD – Document for details

ƒ Links to Agilent Manuals/Videos from Agilent website

*Service Note will be released at later date

© 2008 Waters Corporation 42

 Waters SystemsQT
Maintenance Protocols for Agilent Systems

ƒ Maintenance Procedure Checklist

ƒ Links to Agilent Manuals for details :
— http://www.chem.agilent.com/en-
US/Support/Installation/Pages/LCMaintenanceVideos.aspx

© 2008 Waters Corporation 43

 Waters SystemsQT - Maintenance Protocols
for Agilent Systems Help

ƒ Service Note details links to manuals and videos

ƒ Detailed without graphic of basic maintenance steps
ƒ Current Agilent Part#’s will switch to Waters MP kits

© 2008 Waters Corporation 44

Agilent SystemsQT Operation

ƒ Empower Software AQT recommended to be run before

SystemsQT
— May be run at a separate time – date

ƒ Systems QT Password: Tanasini2007

© 2008 Waters Corporation 45

Details of A1100/1200 SystemsQT

ƒ Qualification Tests
— Test Injections
— System Precision
— Wavelength Accuracy
— Detector Linearity
— Detector Noise and Drift
— Carryover
— Injection Linearity and Accuracy
— Flow Rate Linearity and Accuracy
— Compositional Accuracy (Quaternary Pump only)
— Thermostatted Column Compartment Temperature Accuracy
— Autosampler Temperature Accuracy

© 2008 Waters Corporation 46

Maintenance Protocols
Tools Required

Waters Supplied Tools:

1. Instant Pilot
2. Need Numerous Non-Standard Tools
3. Waters Corporate supplies
4. Specific to Agilent Instruments

© 2008 Waters Corporation 47

Waters Agilent SystemQT Protocol

ƒ Agilent IQ from Node Properties

ƒ Uses Standard Agilent HPLC configuration for test
— Mixer in place
— Tubing in standard configuration
— No Multiple detector configurations

ƒ Uses same general protocol as Alliance 2795 SystemsQT:

— Xbridge 4.6x50 column
— Waters end fittings

ƒ See SystemsQT documentation set

© 2008 Waters Corporation 48

Questions

ƒ Protocol Changes?

ƒ Waters Part #’s

ƒ Service Support

ƒ Other???

© 2008 Waters Corporation 49