Cabina de Seguridad Biologica - Bioare - Nu 480-400e PDF
Cabina de Seguridad Biologica - Bioare - Nu 480-400e PDF
Cellgard ES Energy Saver
Class II, Type A2 Laminar Flow
Biological Safety Cabinet
Models
NU‐480‐300/400/500/600
Bench/Console
Operation & Maintenance Manual
March, 2014
Revision 2
(Series 5 and Higher)
NU-480-400/500/600 Only
Manufactured By:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll‐Free: 1‐800‐328‐3352
In Minnesota: (763)‐553‐1270
Fax: (763)‐553‐0459
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Congratulations!
You have just purchased one of the finest Laminar Flow Biological Safety Cabinets available. With proper care,
maintenance (certification), and laboratory procedure, this cabinet will give you years of product and personnel protection from
particulate contaminants as prescribed in NSF/ANSI 49. Please read this manual carefully to familiarize yourself with proper
installation, maintenance and operation of the cabinet. Other reference and guideline materials are available through the following
web sites.
www.hc‐sc.gc.ca
www.cdc.gov/od/ohs/
www.absa.org
www.absa‐canada.org
www.ebsa.be
www.inspection.gc.ca
www.who.int
www.biosafety.be
www.hse.gov.uk
www.nsf.org
www.cetainternational.org
www.nuaire.com
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ABOUT THIS OPERATION & MAINTENANCE MANUAL
The information contained in this manual is intended to reflect our current production standard configuration model along
with the more frequently purchased options. Any unique additions/modifications/shop drawings are appended in the back flap of
this manual, along with any modifications and/or additions to procedures as outlined in this manual. A copy of the original factory
test report is also appended to this manual. In case this manual and/or test report is lost or misplaced, NuAire retains a copy in our
files. A replacement copy can be obtained by calling or writing NuAire, Inc. stating the model number and serial number and a brief
description of the information desired.
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Cellgard ES Energy Saver
Class II, Type A2 Laminar Flow Biological Safety Cabinet
Models NU‐480‐300/400/500/600
Operation & Maintenance Manual
TABLE OF CONTENTS
Section No. 1 .........................................................General Information
Section No. 2 .........................................................Models & Features
Section No. 3 .........................................................Warranty
Section No. 4 .........................................................Shipments
Section No. 5 .........................................................Installation Instructions
5.1 .........................................................................Location
5.2 .........................................................................Set‐up Instructions
5.3 .........................................................................Testing Methods and Equipment
Section No. 6 .........................................................Operating the NU‐480
6.1 .........................................................................Biological Safety Cabinet Control
6.2 .........................................................................Operating Guidelines
6.3 .........................................................................Operating Sequence
6.4 .........................................................................Ergonomics
6.5 .........................................................................Cleaning Procedures
Section No. 7 .........................................................General Maintenance
7.1 .........................................................................Decontamination
7.2 .........................................................................Fluorescent Lamp Bulb Replacement
7.3 .........................................................................HEPA Filter/Motor Replacement
7.4 .........................................................................Sliding Window Replacement & Manual Adjustment
7.5 .........................................................................Airflow Control System Setup & Calibration
7.6 .........................................................................HEPA Filter Leak test
7.7 .........................................................................Airflow Smoke Pattern Test
7.8 .........................................................................Cleanliness Classification Test for Pharmacy Application
7.9 .........................................................................Main Control Board Description & Replacement
7.10 .......................................................................Digital Airflow Sensor Description & Replacement
Section No. 8 .........................................................Error Messages, Troubleshooting, Option‐Diagnostics &
Airflow Sensor Performance Verification
Section No. 9 .........................................................Remote Contacts
Section No. 10 .......................................................Optional Equipment
10.1 .......................................................................Ultraviolet Light
Section No. 11 .......................................................Electrical/Environmental Requirements
Section No. 12 .......................................................Disposal and Recycle
Insert .....................................................................Replacement Parts
MANUAL DRAWINGS
ACD‐13342..........................................NU‐480 Airflow Schematic
BCD‐13343 ..........................................NU‐480‐300 Specification Drawing
BCD‐13344 ..........................................NU‐480‐400 Specification Drawing
BCD‐13345 ..........................................NU‐480‐500 Specification Drawing
BCD‐13346 ..........................................NU‐480‐600 Specification Drawing
BCD‐11815 ..........................................Drain Valve Installation
ASSEMBLY DRAWINGS
BCD‐05147 ..........................................Base Stand Assembly
BCD‐05146 ..........................................Base Stand Storage Cabinet Assembly
BCD‐11817 ..........................................Control Center & Front Decorative Panel Assembly
BCD‐11686 ..........................................Sliding Window Assembly & Adjustment
BCD‐05153 ..........................................Base Cabinet Assembly
BCD‐12271 ..........................................NU‐480 Window Assembly Hinge Operation
BCD‐12281 ..........................................Auto Window Switch/Cable Adjustment and Maintenance
BCD‐12282 ..........................................Auto Window Cable and Window Assembly
ELECTRICAL SCHEMATICS
BCD‐16581 .........................................NU‐480‐300/400/500/600 Electrical Schematic
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Cellgard ES Energy Saver
Class II, Type A2 Laminar Flow
Biological Safety Cabinet
Models
NU‐480‐300/400/500/600
MANUFACTURED BY:
NuAire, Inc. ‐ Plymouth, Minnesota, U.S.A.
1.0 General Information
1.1 Description
The CELLGARD ES Model NU‐480 Laminar Flow Biological Safety Cabinet (LFBSC) is a bench/table top model, optionally
available with a base support stand, for operation as a console model. The CELLGARD ES model NU‐480 utilizes an Energy
Saver DC ECM motor optimally determined forward curved fan for each model size/width to maximize both energy
efficiency and filter loading capacity. The Energy Saver ECM motor is controlled to airflow setpoints via a solid‐state DC
motor controller with digital dual thermistor airflow sensors that provide an automatic compensation (constant volume
control) for both filter loading and line voltage variances.
The Laminar Flow Biological Safety Cabinet, (LFBSC) is a product resulting from the development of the "laminar flow"
principle (see Airflow Schematic) and the application of environmental controls as required in the field of biological
research or chemical containment. The LFBSC, when used with proper technique, is an effective primary engineering
control in obtaining the optimum product quality while reducing the potential for exposure of both product and personnel
to airborne biological or particulate chemical agents in low to moderate risk‐hazard research and drug preparation or
product operations, as prescribed by the Center for Disease Control (CDC) Atlanta, Georgia.
The NU‐480 bench LFBSC meets the requirements of a Class II, since the cabinet conforms to the following requirements:
1. Maintains a minimum inflow velocity of 100 LFPM (.51mps) through the work access opening
2. Has HEPA filtered downflow air that is mixed with the inflow air from a common exhaust plenum
3. Discharges a percentage of air to the outside atmosphere after HEPA filtration
4. Has all biologically contaminated ducts and plenums under negative pressure or surrounded by negative pressure
Cabinets used for work with minute quantities of non‐flammable or explosive volatile toxic chemicals and tracer amounts
of radionuclides required as an adjunct to microbiological studies must be exhausted through properly functioning exhaust
canopies.
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1.2 Safety Instructions
These safety instructions describe the safety features of the CELLGARD ES Model NU-480 LFBSC.
The safety cabinet has been manufactured using the latest technological developments and has been thoroughly
tested before delivery. However, the cabinet may present potential hazards if it is not installed and used as
instructed for its intended purpose or outside of operating parameters. Therefore, the following procedures
must always be observed:
The safety cabinet must be operated only by trained and authorized personnel.
For any operation of this cabinet, the operator must prepare clear and concise written instructions for
operating and cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical
regulations, in particular.
o which decontamination measures are to be applied for the cabinet and accessories
o which protective measures apply while specific agents are used
o which measures are to be taken in the case of an accident
Repairs to the device must be carried out only by trained and authorized expert personnel.
Keep these operating instructions close to the cabinet so that safety instructions and important
information are always accessible.
Should you encounter problems that are not detailed adequately in the operating instructions, please
contact your NuAire Representative of NuAire technical Services.
Biohazard
Flammable Hazard
Ground, Earth
Chemical Hazard
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2.0 Models & Features
The model NU‐480, CELLGARD ES, Class II, and Type A2 Laminar Flow Biological Safety Cabinet is manufactured in four
sizes: 3 ft. (.9m), 4 ft. (1.2m) , 5 ft. (1.5m) , and 6 ft. (1.8m).
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3.0 Warranty
NuAire, Inc. warrants Class II, Type A2 w/ECM Motor (CELLGARD ES) that it will repair F.O.B. its factory or furnish without
charge F.O.B. its factory a similar part to replace any material including HEPA filters in its equipment within 60 months after
the date of sale if proved to the satisfaction of the company to have been defective at the time it was sold provided that all
parts claimed defective shall be returned, properly identified to the company at its factory, charges prepaid. Factory
installed equipment or accessories are warranted only to the extent guaranteed by the original manufacturer, and this
warranty shall not apply to any portion of the equipment modified by the user. Claims under this warranty should be
directed to NuAire, Inc. setting forth in detail the nature of the defect, the date of the initial installation and the serial and
model number of the equipment.
This warranty shall not apply to any NuAire product or part thereof which has been subject to misuse, abuse, accident,
shipping damage, improper installation or service, or damage by fire, flood or acts of God. If the serial number of this
product is altered, removed or defaced as to be illegible, the Warranty shall be null and void in its entirety.
The warranty is for the sole benefit of the original purchaser and is not assignable or transferable. Prior to returning any
item, for any reason, contact NuAire for a Return Authorization Number. This number must accompany all returns. Any
product shipped to NuAire without this number will be returned refused shipment or collect freight.
4.0 Shipments
NuAire takes every reasonable precaution to assure that your CELLGARD ES cabinet arrives without damage. Motor
carriers are carefully selected and shipping cartons have been specially designed to insure your purchase. However,
damage can occur in any shipment and the following outlines the steps you should take on receipt of a NuAire CELLGARD
ES cabinet to be sure that if damage has occurred, the proper claims and actions are taken immediately.
4.1 Damaged Shipments
4.1.1 Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance.
4.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy
of the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the
basis of your filing the claim against the carrier.
4.1.3 If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE, and request an
inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the
carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain
date and that he failed to comply with the request. This along with other papers in the customer's possession will
support the claim.
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5.0 Installation Instructions
5.1 Location
Within the laboratory, pharmacy, etc., the ideal location of the biological safety cabinet is away from personnel traffic
lanes, air vents (in or out), doors and/or any other source of disruptive air currents.
SUGGESTED CABINET LOCATION IN LABORATORY
THE EXHAUST FILTER AREA IS ESPECIALLY SUSCEPTIBLE TO DISRUPTIVE AIR CURRENTS FROM AIR VENTS. The Electronic
Airflow Control System's exhaust sensor is located just above the exhaust HEPA filter and if disruptive air currents are
present, the exhaust sensor could be influenced by them, and indicate disruptive readings on the front panel display.
If drafts or other disruptive air currents exceed the inflow velocity of the cabinet through the access opening, the potential
exists for contaminated air to exit or enter the work zone area of the cabinet. It depends on the severity of the air current.
REMEMBER: A BIOLOGICAL SAFETY CABINET IS NO SUBSTITUTE FOR GOOD LABORATORY TECHNIQUE.
Where space permits, a clear 6" (152mm) area should be permitted on each side of the cabinet for maintenance purposes.
The electrical outlet into which the cabinet is connected should be readily accessible for maintenance purposes.
Do not position the cabinet to prevent access to the power cord. The power cord plug serves as the disconnect and
should remain readily accessible. If the outlet is inaccessible, such as a conduit (hardwired) connection, then an
appropriate warning label should be applied near the cabinets on/off switch to indicate the circuit breaker on the power
distribution panel should be used. A MINIMUM CLEARANCE OF 6" (152MM) IS REQUIRED FROM THE TOP OF THE CABINET
TO THE CEILING FOR PROPER VENTILATION OF THE EXHAUST EFFLUX.
If this cabinet is used in a pharmacy application, it is strongly recommended (Per OSHA, NIOSH and ASHP) that the cabinet
be exhausted to the outside. In addition, if this cabinet is used in microbiological application with minute quantities of
volatile toxic chemicals and tracer amounts of radionuclides, Per CDC/NIH and NSF it is strongly recommended that the
cabinet be exhausted to the outside. NuAire offers a canopy type of exhaust transition, which will capture the exhaust
efflux from the cabinet.
Canopy, Thimble or Air Gap Exhaust Transitions (with and without integral fan)
Note: THE EXHAUST SYSTEM SHOULD BE FITTED WITH A BACKDRAFT DAMPER
TO PREVENT REVERSING OF AIRFLOW IN THE SYSTEM.
NuAire strongly recommends a canopy or thimble exhaust for most applications. See separate instruction sheets for a
discussion of exhaust transitions and installation requirements.
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5.2 Set‐Up Instructions
Remove outer shipping protection (carton or crating). The cabinet is fastened to the base skid and it is usually the best
procedure to leave the skid in place until the cabinet is located in its approximate position to facilitate ease in handling. It
can then be removed from the skid by removing the banding, bolts and screws holding the cabinet to the skid. It may be
necessary to remove the Control Center in order to gain passage through a doorway. It can easily be removed by following
the instructions on drawing BCD‐11817.
It is recommended that no less than two people are present using a
!
CAUTION
lifting system for placement of the cabinet onto the base stand.
It is not recommended to manually lift the cabinet onto the base stand.
5.2.1 Base Stand Assembly
The base stand is shipped knocked down in a separate carton and is assembled per drawing BCD‐05147 if
accompanied with the cabinet. Remove the banding holding the cabinet to the base skid. Lift the cabinet from
the base skid and place on the floor. Now lift the cabinet on top of the base and bolt the base stand to the cabinet
using two 3/8" ‐ 16 x 3/4" bolts and washers provided for the front base stand tabs and two 1/4" acorn nuts for
the rear weld studs. Place the cabinet in its desired location.
The base stand storage cabinets will usually be shipped according to customer requirements. If it is shipped
unassembled, it can be assembled per drawing BCD‐05146. It is recommended that the upper and lower base
stand braces be installed first, then the rear and bottom panels (the end panels are always prefastened). Once
assembled, fasten the cabinet per the above instructions.
5.2.2 Leveling
Using a level placed on the work tray, adjust the leg levelers, first, end to end, then, front to back. The NSF
approved leg levelers provide a 3/4" (20mm) adjustment.
5.2.3 Bench Installation (BCD‐11815)
Place the cabinet on the bench with approximately a 2" (51mm) overhang clearance for installation of the drain
valve. Place the cabinet in its desired location and using RTV caulk, seal all around the base of the cabinet and the
bench. This provides a tight seal to prevent bench spills from migrating under the cabinet.
If the drain valve is desired, (NOTE, CHECK WITH YOUR SAFETY PERSONNEL FOR REGULATORY REQUIREMENTS
OF DRAIN VALVE INSTALLATION) remove the handle from the valve stem to gain clearance for valve body
rotation. Add Loctite 242 (furnished) to the threads and rotate valve body until secure, with the valve stem (for
handle) on the left side. Re‐install handle to valve stem. Adjust the cabinet on bench to provide a 2" (51mm)
overhang and seal the interface of the bench and cabinet, using RTV caulk as above.
5.2.4 Gas Service
NuAire doesn't recommend the use of natural gas within the BSC, but if gas service is determined to be necessary
for the application, appropriate safety measures must take place. All NuAire BSC's have precautionary warning
labels that say the following:
Use of explosive or flammable substances in this cabinet
should be evaluated by your appropriate safety personnel.
! CAUTION
Once the determination has been made by the appropriate safety personnel, the application of natural gas must
be performed in accordance to national, state and local codes. IT IS ALSO STRONGLY RECOMMENDED THAT AN
EMERGENCY GAS SHUTOFF VALVE BE PLACED JUST OUTSIDE THE BSC ON THE GAS SUPPLY LINE.
All NuAire’s BSC’s meet the safety requirements of UL and CSA for Laboratory Equipment. To comply with these
safety requirements, NuAire uses only certified gas valves. In addition, if external piping is required, only black
pipe is used for this application.
As previously stated NuAire doesn't recommend the use of natural gas within the BSC and ASSUMES NO
RESPONSIBILITY FOR ITS USE. USE AT YOUR OWN RISK. The Bunsen burner flame within the BSC not only
contributes to heat build‐up; is also disrupts the laminar air stream, which must be maintained for maximum
efficiency. IF THE PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER WITH ON DEMAND IGNITION IS
STRONGLY RECOMMENDED. DO NOT USE CONSTANT FLAME GAS BURNERS. During use, the Bunsen burner
should be placed to the rear of the workspace where resulting air turbulence will have a minimal effect.
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5.2.5 Plumbing Services
Service ball valves with the type of service specified by the removable button on the handle are located in the
work zone. The service ball valves are not recommended for pressure over 75 p.s.i. (5.2 BAR). Reducing valves
should be installed external to the cabinet if necessary. Service ball valves should never be used for flammable
gasses or oxygen service. A special needle valve for oxygen service or certified valve is required and available upon
request.
External connection is to 3/8 inch NPT coupling in the inner sidewalls. Connection to plant utilities should be made
with proper materials for the individual service and according to national and/or local codes. Observe all labels
pertaining to the type of service and operating pressure.
5.2.6 Electrical Services
The NU‐480 series Biological Safety Cabinets may be "hardwired" (optional) or plugged into an outlet with
protective earthing connection with the standard power cord. The cabinet requires 115 VAC, 60 Hz, single phase
(current rating varies per cabinet size, reference Electrical/Environmental Requirements). It is recommended that
power to the cabinet whether hardwired or plug connected be on its own branch circuit, protected with a circuit
breaker at the distribution panel near the cabinet.
5.2.7 Final Assembly
Remove the protective cardboard cover over the exhaust HEPA filter, located under the protective screen if in
place. Attach the exhaust sensor shroud over the exhaust sensor. The shroud should be placed as close as
possible to the exhaust HEPA filter without coming in contact. The sensor gasket should be tightly against the
sensor shroud to prevent sneak airflow paths. The exterior surface and viewing glass are easily cleaned with any
mild household detergent cleaner using a soft cloth. Harsh chemicals, solvent‐type cleaners and abrasive cleaners
should not be used.
Do not attempt to clean the HEPA filter media. Cabinet interior walls or work surface are easily cleaned with any
mild household detergent cleaner using a soft cloth. Turn the cabinet on and let it operate for 60 minutes before
using it as a LFBSC.
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5.3 Testing Methods and Equipment
After installation and prior to use, NuAire recommends that the cabinet be tested or commissioned to factory standards.
As part of testing, the certifier should go through the following initial checklist to assure all aspects of the BSC installation
are complete and ready for testing.
Review product installation
‐ Exhaust connection, if present
‐ Damper valve installed correctly with label toward front, if present
‐ BSC basestand level
Verify airflow sensor shroud is in place
‐ Downflow
‐ Exhaust flow
Verify configuration type selection for specific model * (see section 7.5.2)
Verify setpoints and alarm limits for specific model * (see section 7.5.2)
Perform BSC certification
‐ At a minimum, the following tests should be performed:
HEPA filter leak test
Downflow velocity test
Inflow velocity test
Airflow smoke patterns
Site installation assessment tests
The testing methods and equipment required are specified on the factory inspection report included with this manual (see
insert in back cover).
NOTE: IT IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED
TECHNICIAN WHO IS FAMILIAR WITH THE METHODS AND PROCEDURES FOR TESTING BIOLOGICAL
SAFETY CABINETS (SEE INSERT).
NOTE: AFTER THE INITIAL CERTIFICATION, NUAIRE RECOMMENDS THAT THE CABINET BE RECERTIFIED AT A
MINIMUM ON AN ANNUAL BASIS AND AFTER EVERY FILTER CHANGE OR MAINTENANCE ACTION OR ANY
TIME THE OPERATOR FEELS IT IS NECESSARY.
Note that the CELLGARD ES cabinets, filters and seals provide premium performance; Quality Control in both design and
manufacturing assure superior reliability. However, protection to both product and operator is so vital that installation
testing to the performance requirements should be accomplished as stated to ensure biological safety established by the
factory standards.
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Cellgard ES Energy Saver Class II Type, A2
Laminar Flow Biological Safety Cabinet
Models NU‐480‐300/400/500/600
Catalog Number
Catalog Number NU‐480‐300 NU‐480‐400 NU‐480‐500 NU‐480‐600
Nominal 3 foot (0.9m) Nominal 4 foot (1.2m) Nominal 5 foot (1.5m) Nominal 6 foot (1.8m)
Performance Specifications
NSF/ANSI 49 NSF/ANSI 49 NSF/ANSI 49 NSF/ANSI 49
1. Personal Protection
2. Product Protection
NSF/ANSI 49 Class II, Type A2 Class II, Type A2 Class II, Type A2 Class II, Type A2
Bench top/console Bench top/console Bench top/console Bench top/console
Style of Cabinet w/base stand/storage w/base stand/storage w/base stand/storage w/base stand/storage
cabinet cabinet cabinet cabinet
All welded stainless steel All welded stainless steel All welded stainless steel All welded stainless steel
Cabinet Construction 16GA, Type 304 pressure 16GA, Type 304 pressure 16GA, Type 304 pressure 16GA, Type 304 pressure
tight design tight design tight design tight design
Diffuser for Air Supply (Metal) Non‐flammable Non‐flammable Non‐flammable Non‐flammable
HEPA Filter Seal Type:
Supply Filter‐99.995% Eff. on 0.3 microns HEPEX Seal HEPEX Seal HEPEX Seal HEPEX Seal
Exhaust Filter‐99.995% Eff. on 0.3 microns Neoprene, Spring‐loaded Neoprene, Spring‐loaded Neoprene, Spring‐loaded Neoprene, Spring‐loaded
Fumigation : per NIH/NSF Procedures Yes Yes Yes Yes
Standard Services:
Service Coupling (3/8 inch NPT) One, Right Sidewall One, Right Sidewall One, Right Sidewall One, Right Sidewall
Gas Valve/Service Coupling (3/8inch NPT) One, Right Sidewall One, Right Sidewall One, Right Sidewall One, Right Sidewall
Duplex Outlet Two, Backwall Two, Backwall Two, Backwall Two, Backwall
Optional Services:
Gas Cocks 3/8" NPT Up to 3 ea. Sidewall Up to 3 ea. Sidewall Up to 3 ea. Sidewall Up to 3 ea. Sidewall
Ultraviolet Light One, Backwall One, Backwall One, Backwall One, Backwall
Standard/Cup Sinks Left or Right Work Left or Right Work Left or Right Work Left or Right Work
Surface Surface Surface Surface
Cabinet Size Inches (mm):
Height (Fully Assembled) 61 7/8 (1572) 61 7/8 (1572) 61 7/8 (1572) 61 7/8 (1572)
Height (Minimum for Transport) Width 59 (1499) 59 (1499) 59 (1499) 59 (1499)
Depth (with Control Center) 41 5/8 (1057) 53 5/8 (1362) 65 5/8 (1669) 77 5/8 (1972)
And Armrest Removed 33 (838) 33 (838) 33 (838) 33 (838)
Work Access Opening Inches (mm):
Standard Opening Height/Optional 8 (203) 10 (254) / 8 (203) 10 (254) / 8 (203) 10 (254) / 8 (203)
Standard Inflow Velocity 105 FPM (.53 m/s) 105 FPM (.53 m/s) 105 FPM (.53 m/s) 105 FPM (.53 m/s)
Work Zone Inches (mm):
Height 28 1/2 (724) 28 1/2 (724) 28 1/2 (724) 28 1/2 (724)
Width 34 3/8 (873) 46 3/8 (1178) 58 3/8 (1483) 70 3/8 (1788)
Depth measured at 10 inches (254mm) 26 (660) 26 (660) 26 (660) 26 (660)
window height
Viewing Window Inches (mm): Fully closed to Fully closed to Fully closed to Fully closed to
Standard is safety plate sliding glass 18 (457) open 18 (457) open 18 (457) open 18 (457) open
Required Exhaust CFM/CMH 8 (203) opening 10(254) / 8(203) opening 10(254) / 8(203) opening 10(254) / 8(203) opening
Standard/Optional: CFM (CMH) CFM (CMH) CFM (CMH) CFM (CMH)
Canopy Variable Flow Thimble (NU‐911) 225‐450 (383‐765) 363‐588 (617‐1000) / 451‐676 (766‐1149) / 538‐763 (915‐1297) /
295‐520 (502‐884) 365‐590 (621‐1003) 436‐661 (741‐1124)
Canopy Fixed Flow Thimble (NU‐907) 269 (457) 426 (724) / 359 (610) 531 (902) / 445 (756) 634 (1077) / 532 (904)
Plant Duct Static Pressure Eng./Metric 0.05‐0.1"/1.27‐2.54mm 0.05‐0.1"/1.27‐2.54mm 0.05‐0.1"/1.27‐2.54mm 0.05‐0.1"/1.27‐2.54mm
H2O H2O H2O H2O
Heat Rejected, BTU, Per Hour 8 (203) opening 10(254) / 8(203) opening 10(254) / 8(203) opening 10(254) / 8(203) opening
(non‐vented) 826 1140/1020 1768/1611 1884/1768
(vented) 120 157 198 198
Electrical: U.L./U.L.‐C Listed U.L./U.L.‐C Listed U.L./U.L.‐C Listed U.L./U.L.‐C Listed
Volts, AC 60 Hz 115 115 115 115
+Amps: Blower/Lights (10/8 openings) 2.1 2.9/2.6 4.5/4.1 4.8/4.5
Amps: Duplex 3 3 3 3
Rated Amps: 10 12 14/*12 14/*12
12 ft. Power Cord (one) 14 GA ‐ 3 Wire, 15A 14 GA ‐ 3 Wire, 15A *12/14 GA‐3 Wire, *12/14 GA‐3 Wire,
20A Std., 15A Optional 20A Std., 15A Optional
Crated Shipping Weight:*** 475 lbs. /215 kg. 550 lbs. /249 kg. 650 lbs. /295 kg. 730 lbs. /331 kg.
Net Weight 425 lbs. /193 kg. 500 lbs. /227 kg. 600 lbs. /273 kg. 680 lbs. /308 kg.
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6.0 Operating the NU‐480
6.1 Biological Safety Cabinet Control
6.1.1 Overview
The Biological Safety Cabinet Control (BSCC) system is designed to service the control requirements of the NU‐480
Biological Safety Cabinet. The control system is a self‐contained microprocessor driven module that will perform
the following functions:
Easy user interface via TOUCHLINK LCD
Control DC ECM motor/blower via solid state DC motor controller
Monitor, display and control downflow, via digital dual thermistor airflow sensor
Monitor and display exhaust flow (inflow) via digital dual thermistor airflow sensor
Alarm setpoints, high/low for error conditions (downflow and exhaust flow)
Date/Clock display and timer function
Control lights via solid state switch
Control outlets via solid state switch
Complete diagnostic functions
The NU‐480 BSCC system offers the latest dual microprocessor design technology for improved cabinet
performance and safety. The control system uses a digital dual thermistor airflow sensor in the downflow stream
to monitor and control airflow to setpoints. The control system automatically compensates for filter loading,
voltage variances and other environmental effects. A second digital dual thermistor airflow sensor in the exhaust
airstream monitors for inflow velocity. Both downflow and inflow are displayed on the TOUCHLINK LCD screen.
The control system also monitors the sliding window position with a micro switch for both window height and
window closed positions.
The control system though the use of the front panel controls the on/off function of the fluorescent and
ultraviolet lights (optional), outlets and DC ECM motor/blower. The control system also allows contact closure
outputs for interaction with HVAC systems to optimize environmental performance.
User interface to the BSCC system is accomplished via the TOUCHLINK LCD. Basic use of the BSC is accomplished
via the icons located along the top of the screen as shown below. Touch an icon to turn on/off functions as
indicated. Each icon will illuminate with color to indicate when the function is turned on. The menu icon will
always prompt a menu screen to display. Selecting a menu item will continue the prompts until the desired
parameter is achieved. To return to the main menu, press the MENU icon repeatedly to reverse out of the
parameter menus.
MENU
UV
FL
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6.1.2 Standby Mode
When the BSC is not in use, the TOUCHLINK LCD screen will display a large NuAire logo, the icons along the top
and the time and date at the bottom right as shown below. Any of the function icons, except the blower, that
initiates Run Mode, may be turned on and off in standby mode. The timer and menu icons may also be accessed
for additional user menus. The TOUCHLINK LCD does have a screen saver function built in for extended LCD life.
The default screen saver time is 60 minutes. This means after 60 minutes when the blower is not on, the
TOUCHLINK LCD will go dark. To bring back the TOUCHLINK LCD, just touch the screen and the screen saver will
reset. To change the screen saver time, access SCREEN SETUP through the menu icon.
MENU
UV
FL
xx:xx AM xx/xx/xx
6.1.3 Run Mode
Any time the blower icon is selected the RUN MODE screen will appear. The Run Mode screen will display a BSC
profile and initiate and display the countdown of a 150 second warm‐up period. However, the aseptic cleaning
process may begin if the sliding window is raised an audible and visual alarm will occur but may be silenced by
pressing the alarm silence icon that appears. Airflow readings will not be displayed during the warm‐up period.
MENU
UV
FL
0.0 fpm
xx:xx AM xx/xx/xx
Once the warm‐up period is complete, airflow readings and all system functions will operate and be displayed.
6.1.4 Night Setback/ Nite Care Mode
The NU‐480 may be optionally configured to allow the DC ECM motor to continue to run at a lower rate with the
sliding window closed allowing the workzone interior to be continually HEPA filtered. If the night setback mode is
configured, the blower icon must be on and the window closed for it to be activated. In addition, the fluorescent
light will be disabled. If the sliding window is then opened the cabinet will resume normal airflow setpoints in run
mode.
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6.1.5 Standby/Run Mode Alarms
If present standby/run mode alarms will be both visual and audible, the Red LED oval under the LCD display will
turn on, and the TOUCHLINK LCD screen will also display a description of the alarm in place of the NuAire Logo.
Depending upon the alarm type, the BSC profile will also indicate in red the alarm present. Audible alarms can be
silenced or will produce an alarm tone for 30 seconds, then into a ring back cycle of once every 10 seconds.
Pressing the alarm silence icon will silence the audible alarm for 15 minutes then into a ring back cycle of once
every 10 seconds
MENU
UV
FL
ACTIVE ALARMS!
WINDOW HIGH!
60 fpm
105 fpm
xx:xx AM xx/xx/xx
Alarm Types
Window High ‐ window is raised above its nominal height
Window Low ‐ window is lowered below its nominal height (used only with power window option)
Downflow High Limit‐ downflow is above the high alarm setpoint
Downflow Low Limit ‐ downflow is below the low alarm setpoint
Inflow High Limit ‐ inflow is above the high alarm setpoint
Inflow Low Limit ‐ inflow is below the low alarm setpoint
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6.1.6 Timer Icons
The timer icon, when pressed will provide a list of time functions available for use.
Below is a description of each timer function.
MENU
UV
FL
LABORATORY TIMER UP
PURGE TIMER
DOWN
OUTLET TIMER
UV LIGHT TIMER
ENTER
AUTO RUN TIMER
xx:xx AM xx/xx/xx
Timer Functions
Laboratory Timer ‐ A general purpose timer that when set to a value, will countdown and alarm upon timer
expiration.
UV
MENU
FL
LABORATORY TIMER
UP
CURRENT SETTING = 0.00 MINS. SECS.
TIME REMAINING = 0.00 MINS. SECS.
DOWN
THE LABORATORY TIMER IS A GENERAL
PURPOSE TIMER THAT WHEN SET TO A VALUE,
START
WILL COUNTDOWN AND AUDIBLE ALARM
UPON TIMER EXPIRATION. PRESS UP AND
DOWN TO CHANGE THE SETTING. PRESS
START TO INITIATE THE TIMER.
xx:xx AM xx/xx/xx
Purge Timer ‐ This timer controls how long the blower will run to purge the cabinet after the blower icon has
been pressed to turn off the blower.
MENU
UV FL
PURGE TIMER UP
CORRECT SETTING = 0.00 MINS. SECS.
DOWN
THIS TIMER CONTROLS HOW LONG THE
BLOWER WILL RUN TO PURGE THE CABINET
AFTER THE BLOWER ICON HAS BEEN PRESSED SAVE
TO TURN OFF THE BLOWER. PRESS UP AND
DOWN TO CHANGE THE SETTING. PRESS SAVE
TO SAVE NEW SETTING.
xx:xx AM xx/xx/xx
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Outlet Timer ‐ This timer controls how long the outlet remains on after the outlet icon has been pressed to
turn on the outlet. If timer is zero, the outlet will stay on until turned off.
MENU
UV
FL
OUTLET TIMER UP
CURRENT SETTING = 0.00 MINS. SECS.
DOWN
THIS TIMER CONTROLS HOW LONG THE OUTLET
REMAINS POWERED AFTER IT TURNS ON. THE
OUTLET WILL AUTOMATICALLY TURN OFF SAVE
WHEN THE TIMER EXPIRES. PRESS UP AND
DOWN TO CHANGE THE SETTING. PRESS SAVE
TO SAVE NEW SETTINGS.
xx:xx AM xx/xx/xx
UV Light Timer ‐ This timer controls how long the UV light will remain on after the UV light icon has been
pressed to turn on the UV light. If timer is zero, UV light will stay on until turned off.
MENU
UV
FL
UV LIGHT TIMER UP
CURRENT SETTING = 0.00 MINS. SECS.
DOWN
THIS TIMER CONTROLS HOW LONG THE UV LIGHT
REMAINS ON AFTER IT TURNS ON. THE UV LIGHT
WILL AUTOMATICALLY TURN OFF WHEN THE SAVE
TIMER EXPIRES. PRESS UP AND DOWN TO CHANGE
THE SETTING. PRESS SAVE TO SAVE NEW SETTING.
xx:xx AM xx/xx/xx
Auto Run Timer ‐ This timer provides the ability to program on a daily basis the start and stop time of the cabinet.
To start and stop the cabinets menus that both the blower and fluorescent lights will
automatically turn on and off together on a programmed schedule. Once into the auto timer
menu, select the desired day for the auto timer to function. If multiple days are desired, each
day will be required to be set individually.
MENU
UV
FL
MONDAY
TUESDAY UP
WEDNESDAY
THURSDAY DOWN
FRIDAY
SATURDAY
ENTER
SUNDAY
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Once into the selected day, press UP or DOWN to enter the on/off times. Use the >> and << to select hours or
minutes. Press SWITCH to toggle between auto time on and auto time off. Press SAVE after each time entry.
Press DIS to disable auto timer for the day being reviewed. Repeat auto timer function for each day as desired.
MENU
UV
FL
AUTO TIME ON UP
SETTING MONDAY
<< DOWN
>>
SAVE
SWITCH
PRESS SWITCH TO TOGGLE BETWEEN AUTO ON AND OFF TIMES DIS
PRESS DIS TO DISABLE THIS ON TIME
USE >> AND << TO SELECT HOURS OR MINUTES
USE UP AND DOWN TO CHANGE TIME
xx:xx AM xx/xx/xx
6.1.7 Menu Icon
The menu icon, when pressed will provide a list of menu items for various BSCC functions.
MENU
UV
FL
UP
CALIBRATION/SERVICE
TIME/DATE
DOWN
PASSWORD
CABINET DECONTAMINATION ENTER
SCREEN SET-UP
FILTER STATUS
xx:xx AM xx/xx/xx
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Menu Items
Calibration/Service ‐ A password protected area used by certification or service personnel to set up and
calibrate the cabinet for certification or commissioning.
Time/Date ‐ This menu item provides the ability to set the time and date displayed on the LCD screen.
Time displayed is real time and will not automatically adjust for day light saving time.
MENU
UV
FL
UP
SET TIME
DOWN
SET DATE
ENTER
xx:xx AM xx/xx/xx
MENU
UV
FL
SETTING CLOCK TIME
UP
<<
>> X:XX AM DOWN
SWITCH
SAVE
PRESS SWITCH TO TOGGLE BETWEEN 12 OR 24
HR DISPLAY. USE >> AND << TO SELECT HOUR
OR MINUTES. USE UP AND DOWN TO CHANGE
TIME. PRESS SAVE TO SAVE NEW SETTING.
xx:xx AM xx/xx/xx
MENU
UV
FL
SET CURRENT DATE
UP
<< MM / DD / YYY
DOWN
>>
USE >>
AND << TO SELECT MONTH, DAY OR YEAR. SAVE
USE UP AND DOWN TO CHANGE VALUE.
PRESS SAVE TO SAVE NEW SETTING. xx:xx AM xx/xx/xx
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Password ‐ This menu item provides the ability to change the user password from the default value of 1234.
MENU
UV
FL
UP
SET PASSWORD DOWN
DEFAULT PASSWORD ENTER
xx:xx AM xx/xx/xx
Set Password
EXIT
MENU
0 5
1 6
2 7 ENTER OLD PASSWORD BACK
XXXX
3 8
ENTER
4 9
xx:xx AM xx/xx/xx
Default Password
MENU
UV
FL
DEFAULT PASSWORD DEFAULT
PRESSING DEFAULT WILL SET THE
WORKING PASSWORD BACK TO THE
FACTORY DEFAULT SETTING OF 1234.
xx:xx AM xx/xx/xx
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MENU
EXIT
UV
0 5
FL
1 6
7 BACK
2
ENTER NEW PASSWORD
XXXX
3 8 SAVE
4 9
xx:xx AM xx/xx/xx
Decon Cycle ‐ This menu item provides the ability to perform an automated or manual decon procedure.
(See decontamination section for instructions).
Screen Set‐Up ‐ This menu item provides the ability to alter LCD screen display background
contrast and audible touch screen tone.
MENU
UV
FL
UP
SET BACKGROUND COLORS
DOWN
TOUCH DISPLAY TONE
SCREEN SAVER ENTER
xx:xx AM xx/xx/xx
MENU
UV
FL
SET MAIN BACKGROUND COLOR UP
SET BORDER COLOR
DOWN
SAVE
xx:xx AM xx/xx/xx
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MENU
UV
FL
SETTING MAIN SCREEN BACKGROUND COLOR
SELECT COLOR BELOW TO CHANGE
WHITE
GREEN
BLUE
GRAY
xx:xx AM xx/xx/xx
MENU
UV
FL
SETTING BORDER COLOR
SELECT COLOR BELOW TO CHANGE
WHITE
GREEN
BLUE
GRAY
xx:xx AM xx/xx/xx
MENU
UV
FL
ON
TOUCH DISPLAY TONE IS ON
WHEN THOUCHSCREEN TONE IS ON, AN
OFF
AUDIBLE TONE WILL SOUND TO INDICATE A
BUTTON PRESS. WHEN OFF IT WILL NOT
SOUND.
USE ON AND OFF TO CHANGE SETTING. PRESS
SAVE
ENTER TO SAVE NEW SETTING.
xx:xx AM xx/xx/xx
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MENU
UV
FL
SCREEN SAVER
OPTION SETTING 60
ON
THIS OPEION DETERMINES HOW LONG THE
DISPLAY WILL REMAIN ACTIVE AFTER THE
OFF
BLOWER IS TURNED OFF.
VALID RANGE IS 0 - 240 MINUTES.
SETTING THE VALUE TO 0 TURNS THE SCREEN
SAVER OFF. PRESS UP/DOWN TO CHANGE THE
SAVE
VALUE. PRESS SAVE TO SAVE THE SETTING. xx:xx AM xx/xx/xx
Filter Status ‐ This menu item provides the status of available filter life.
FILTER LIFE AVAILABLE XX% MENU
GREEN AREA RED AREA
PRESS MENU TO EXIT
xx:xx AM xx/xx/xx
6.1.8 Power Window Option
The power window option, if purchased, is factory installed and the display will indicate window up and down
icons just above the time and date. The power window function uses a 24 Vdc motor and internal feedback
encoder to determine position. The power window also incorporates a safety auto‐reverse feature to eliminate
any pinch hazard.
Operating the power window is performed by pressing either the UP or DOWN window icon. The travel is
determined by the length of time the icon is pressed. A short press and release will make the window move a
short distance. A long press and release will make the window move to the next standard operating point of
either closed, nominal work access height of 10 inches (254mm) or full open. The power window is required to be
lowered during the decontamination process for sealing purposes after the armrest is removed.
MENU
UV
FL
xx:xx AM xx/xx/xx
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6.2 Operating Guidelines
The intent herein is to present general operational guidelines that will aid in the use of the Laminar Flow Biological Safety
Cabinet (LFBSC) to control airborne contaminants of low to moderate risk as stated in Technical Report Number
FPS 56500000001, prepared by Dow Chemical U.S.A. for the National Cancer Institute, May 1, 1972.
Procedure protocols defined in terms of the barrier or control concepts unique to LFBSC must be developed in order to
obtain a maximum potential for safety and protection. The pre‐planning necessary to develop these protocols is based on
several fundamental considerations, each of which will contribute to optimum benefits from the equipment:
a. Know you “Safe Work Area”
b. Minimize disruption of “air curtain”
c. Minimize room activity
d. Utilize unidirectional airflow
e. Employ aseptic techniques
6.2.1 Know about your "Safe Work Tray Area"
The LFBSC safe working area is basically the worktray or depressed area. All work should be performed on or
above the worktray. The area on or above the front grill is a non‐safe working area.
NOTE: It is important to maintain an air gap on both sides of the worktray before fastening in place.
The work tray as being part of the cabinet system has been designed to load up to 100 lbs. (45.4 kg)
of work materials. Any additional loading should be evaluated by appropriate safety personnel.
6.2.2 Minimize Penetration of "Air Curtain"
The minimum number of items necessary should be placed into the cabinet to prevent overloading, but the work
should also be planned to minimize the number of times an operator's hands and arms must enter and leave the
air curtain at the open face. The ideal situation is to have everything needed for the complete procedure placed in
the hood before starting, so that nothing need pass in or out through the air barrier at the face until the procedure
is completed. This is especially important in working with moderate risk agents.
Unnecessary raising of the hands inside the cabinet above the level of the work opening should be avoided. This
presents an inclined plane from hands to elbows along which the downflow of air may run to, and possibly out, the
open face.
NOTE: When working with agents of lower risk, it is not as important for all materials to be placed in the
cabinet before starting, or for the procedure to be completely finished before materials are removed.
Also, the time period for cabinet may be continued over a more extended period during which entries
and withdrawals from the cabinet may be made.
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6.2.3 Minimize Room Activity
Activity in the room itself should be held to a minimum. Unnecessary activity may create disruptive air currents as
well as interfere with the work of the operator. A person walking past the front of a cabinet can cause draft
velocities up to 175 fpm (.89 m/s), which are sufficient to disrupt the air balance of the laminar flow cabinet.
6.2.4 Utilize Unidirectional Air Flow
The operator must keep two important facts in mind: (1) The air, as supplied to the work area through filters from
the top, is contaminant free and (2) Airborne contamination generated in the work area is controlled by the
unidirectional flow of parallel air streams in a top‐to‐bottom direction.
A solid object placed in a laminar air stream will disrupt the parallel flow and consequently, the capability of
controlling lateral movement of airborne particulates. A cone of turbulence extends below the object and
laminarity of the air stream is not regained until a point is reached downstream, approximately equal to three to
six times the diameter of the object. Within the parameters of this cone, particles may be carried laterally by
multidirectional eddy currents.
Transfer of viable materials and manipulations, which may generate aerosols, should not be performed above
sterile or uninoculated materials. Items should be localized on the work surface in "clean" and "dirty" groups.
6.2.5 Employ Aseptic Technique
The operator must not assume an attitude of "let the cabinet do it" when performing procedures within a LFBSC.
Properly balanced and properly used cabinets will do an excellent job of controlling airborne contamination and
containing viable agents, but the cabinet will not eliminate contact transmission of contamination. Normal
laboratory contamination control procedures and basic aseptic techniques are necessary to obtain maximum
benefit from the cabinet. For example, open bottle, tube or flask mounts should be kept as parallel as possible to
the downflow to minimize capture of chance particulates. This precaution is merely an extension of good aseptic
technique as practiced on open bench tops. The good laboratory practices designed to minimize creation and/or
release of aerosols to the environment should not be discontinued.
Items of equipment in direct contact with the etiologic agent must remain in the cabinet until enclosed or until
surface‐decontaminated. Trays of discard pipettes must be covered before removal from the cabinet (aluminum
foil may substitute for fabricated covers).
If an accident occurs which spills or splatters suspensions of etiologic agent around the work area, all surfaces and
items in the cabinet must be surface‐decontaminated before being removed.
Applying a burner flame to flask and tube necks when mating surfaces of sterile assemblies is a conventional
method of minimizing chance contamination. However, the efficiency of this operation is usually related to the
removal of airborne contamination occurring while the item is uncovered. If the manipulation is carried out in an
environment free of airborne particulates, then the need for the flaming operation is essentially removed. This is
one of the additional advantages of the LFBSC ‐ use of the gas burner is seldom necessary. The gas burner flame in
one of these cabinets not only contributes significantly to the heat build‐up, it also disrupts the laminar air streams
which must be maintained for maximum efficiency. If the procedure demands use of a flame, A BUNSEN BURNER
WITH ON DEMAND IGNITION IS RECOMMENDED. DO NOT USE CONSTANT FLAME GAS BURNERS. It should also
be only used from the center of the work surface to the right rear where resulting air turbulence will have a
minimal effect. DO NOT USE GAS BURNER ON THE LEFT SIDE OF THE WORK SURFACE DUE TO ITS INFLUENCE ON
THE ELECTRONIC AIRFLOW CONTROL SYSTEM. If cabinet air is inadvertently turned off, the flame could damage
the HEPA filters.
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6.3 Operating Sequence
6.3.1 Start Up
Turn on cabinet blower and lights, check air intake and exhaust portals of the cabinet to make sure they are
unobstructed. The electronic airflow control system will automatically control airflows to specified setpoints.
However, upon filter loading, the cabinet may be required to be re‐balanced or filters replaced. Only a qualified
maintenance technician should perform cabinet balancing and filter replacement.
NOTE: Some cabinets are equipped with ultraviolet (UV) lights. Good procedure includes the decontamination
or wipe down of cabinet surfaces with chemical disinfectant before work commences. This practice
eliminates the need for UV lights, whose primary utility in this application is inactivation of surface
contamination since the filters effectively remove all airborne contaminants. UV lights, therefore, are not
recommended in the LFBSC.
Allow blowers to operate for a minimum of 15 minutes before aseptic manipulations are begun in the
cabinet. If the filtered air exhausted from the cabinet is discharged into the room, as in some
installations, an additional advantage is obtained from purification (filtration) of the room air circulated
through the equipment. Because of this characteristic contributing to the quality of the laboratory
environment, some owners of LFBSC leave them in operation beyond the time of actual use.
6.3.2 Wipe Down
The interior surfaces of the workspace should next be disinfected (see Cleaning Procedures) by wiping them
thoroughly with 70% alcohol or similar non‐corrosive anti‐microbial agents. USE OF CHLORINATED OR HALOGEN
MATERIALS IN THE CABINET MAY DAMAGE STAINLESS STEEL.
6.3.3 Materials & Equipment
The apparatus and materials should next be placed into the cabinet. Care must be exercised that no items be
placed over the front intake grills. Materials should be arranged so that clean, dirty (used), and virus materials are
well separated. Passage of contaminated materials over uninoculated cultures or clean glassware should be
avoided and transfer of viable materials should be performed as deeply into the cabinet (away from open face) as
possible.
6.3.4 Air Purge
Additional purging of the workspace without user activity should be allowed for 2‐3 minutes after materials and
apparatus have been placed in it. This will rid the area of all "loose" contamination that may have been
introduced with the items.
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6.3.5 Perform Work
The work can now be performed. The technician performing the work is encouraged to wear a long‐sleeved gown
with knit cuffs and rubber gloves. This will minimize the shedding of skin flora into the work area and concurrently
protect the hands and arms from viable agent contamination. At a minimum, the hands and arms should be
washed well with germicidal soap before and after work in the cabinet. For the preparation of antineoplastic
drugs, the following procedures summarize those contained in OSHA Technical Manual TED 1‐0.15A, Section VI,
Chapter 2 “Controlling Occupational Exposure to Hazardous Drugs”. The above document should be thoroughly
studies and reviewed prior to drug preparation in the cabinet. It may be found at this website.
http://www.osha.gov/dts/osta/.
a. A sterile plastic‐backed absorbent drape should be placed on the work surface during mixing procedures.
The drape should be exchanged whenever significant spillage occurs, or at the end of each production
sequence.
b. Vials should be vented with a filter needle to eliminate internal pressure or vacuum.
c. Before opening ampoules, care should be taken to insure that no liquid remains in the tip of the ampoule.
A sterile gauze sponge should be wrapped around the neck of the ampoule while opening.
d. Final drug measurement should be performed prior to removing the needle from the stopper of the vial.
e. A non‐splash collection vessel should be available in the biological safety cabinet to discard excess drug
solutions.
6.3.6 Terminal Purging & Wipe Down
Following completion of work, allow the cabinet to run for 2‐3 minute period without personnel activity to purge
the cabinet. A surface disinfection of the interior surfaces (see Cleaning Procedures section) should be repeated
after removal of all materials, cultures, apparatus, etc. A careful check of grills and diffuser grids should be made
for spilled or splashed nutrients which may support fungus growth and resulting spore liberation that
contaminates the protected work environment.
6.3.7 Paper Catch/Prefilter
A permanent paper catch is installed behind the rear divider panel of the work zone. This area forms the return air
path to the motor/blower; and if the airflow is blocked, it could seriously affect the performance of the cabinet.
Therefore, THE PAPER CATCH SHOULD BE CHECKED AND CLEANED NO LESS THAN ON A WEEKLY BASIS; daily
basis if procedures dictate the use of paper products. Any paper removed must be properly disposed of as
Contaminated Hazardous Waste. The above procedures also apply to all cabinets configured with a prefilter.
6.3.8 Shut Down
Turn off blowers and lights. Do not use cabinet as a depository for excess lab equipment during periods of non‐
operation. If antineoplastic agents are being prepared in the cabinet, it is recommended to let the cabinet run 24
hours per day. This lessens the possibility that contaminants may escape.
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6.4 Ergonomics
Ergonomics, the study or accommodation of work practices is extremely important for proper cabinet usage and user
health and safety. An evaluation of normal work practices should be performed with each user when working in a cabinet.
Evaluation criteria should be at a minimum:
a. Proper user posture
b. Effective work zone layout for work practice
c. Vision or sightlines
For each of the above evaluation criterion, several work aids may be supplied to accommodate the user.
Ergonomic chair ‐ A six‐way articulating seat and back control for personalized adjustment to assure proper user
posture. Be sure feet are resting on the floor, chair foot support or foot rest. Also be sure back is fully supported
with proper chair adjustments.
Forearm/elbow support ‐ The cabinet is provided with a non‐metallic forearm support on the work access
opening. Periodic mini‐breaks during work practice should be taken resting forearm to avoid stress and fatigue.
Elbow rests are optional that can provide support for particular work practices, such as pipetting. Also available as
an option, closed cell foam disposable forearm pads to reduce pressure points and add comfort.
Effective workzone layout ‐ Always prepare your work procedure to minimize reach to avoid neck and shoulder
stress and fatigue. Rotating tables are optional to maximum workzone and minimize reach.
Vision and sightline ‐ Always prepare your work procedure to eliminate glare and bright reflections on the window.
Keep your window clean and sightlines clear to your effective workzone.
6.5 Cleaning Procedures
Cleaning the cabinet is an important function in terms of both containment and sterility. Use the following procedure to
effectively clean or surface disinfect the cabinet workzone surfaces.
a. Raise the sliding window to a full‐open position, if desired.
b. Press the cleaning key on the front control panel to silence the audible alarm during the cleaning process.
c. Apply appropriate disinfecting solution (i.e. coverage plus ™ (Calgon Corp.)) or similar disinfectant to cabinet
surfaces. Most surface disinfectants require a specific contact time depending upon the microbiological agents
used within the cabinet. CONSULT APPROPRIATE DISINFECTANT DOCUMENTATION FOR PROPER APPLICATION
AND SAFETY PRECAUTIONS.
DISINFECTANTS THAT USE CHLORIDES AND HALOGENS WILL CAUSE DAMAGE
! CAUTION
TO THE STAINLESS STEEL SURFACES IF LEFT ON FOR LONG PERIODS OF TIME.
d. After the specified contact time, wipe up excess disinfectant. IF THE DISINFECTANT USED CONTAINS CHLORIDES
OR HALOGENS, RE‐WIPE ALL SURFACES WITH 70% ALCOHOL OR SIMILAR NON‐CORROSIVE ANTI‐MICROBIAL
AGENT TO PREVENT DAMAGE TO STAINLESS STEEL SURFACES.
6.6 Hazardous Drug Decontamination Procedures
This procedure is intended to provide guidance following a spillage and/or periodic maintenance, testing or relocation of
the cabinet. Additional guidance can be provided by the CETA document CAG‐005‐2007 found at the CETA website:
www.CETAinternational.org.
6.6.1 Preparation
Prior to beginning decontamination activity, personnel should wear proper personnel protection equipment (PPE)
1
i.e. Tyvek isolation gown, 2 pair of Nitrile gloves and a full‐faced HEPA filtered respirator. All protective garments
should be contained in 4 mil plastic bags and labeled for disposal as chemotherapy waste after completion of the
procedure. For the purpose of this procedure, detailed procedures for cleaning a Class II BSC can be found in the
2006 ASHP Technical Assistance Bulletin ASHP Guidelines on Handling Hazardous Drugs2.
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6.6.2 Procedure
a. Make sure that the cabinet remains in operational mode with internal blower on.
b. Open the hinged or sliding view screen and secure in the full open position.
With the view screen in the full open position, personnel protection
!
CAUTION
is compromised and a full faced HEPA filtered respirator must be
worn.
c. Clean all readily accessible surfaces of the cabinet.
d. Remove perforated metal diffuser screen from the underside of the supply HEPA filter and place on the
cabinet work tray.
NOTE:
Depending on the model, the diffuser screen is secured to the cabinet by #8‐32 screws or
1/4" ‐ 20 acorn nuts, 3 places. It is purposely a tight fit and is secured to the back wall with
projecting threadless studs.
e. Clean both sides of the perforated metal diffuser screen and remove it from the cabinet.
f. Lift the cabinetwork tray, clean both sides and remove it from the cabinet.
g. Remove the front perforated grill, place on the cabinet floor and clean both sides.
Remove from cabinet.
h. Clean work tray supports.
i. Working from top to bottom, clean all inside surfaces of the cabinet. Take care not to wet the HEPA filter.
If liquid has collected in the plenum drain, aspirate it using IV tubing into an evacuated container. Label
the evacuated container for disposal as chemotherapy waste.
j. Clean the plenums drain area and wipe dry.
k. If the cabinet requires maintenance and/or replacement of the HEPA filters, the operation should be
halted at this point to allow trained personnel to complete replacement of the HEPA and/or maintenance
action required.
6.6.3 Assembly
a. Replace front (if removed) grill.
b. Replace the work tray and carefully tighten the thumbscrews.
c. Replace perforated metal diffuser screen over the underside of the supply HEPA filter.
d. Wipe down all exposed surfaces of the work area with 70% isopropyl alcohol.
e. Prepare for aseptic operation.
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7.0 General Maintenance
All maintenance actions on this equipment must be performed by a qualified
technician who is familiar with the proper maintenance procedures required
! CAUTION for this equipment. This includes both certification as well as repair.
7.1 Decontamination
No maintenance should be performed on the interior of the CELLGARD ES cabinet (area behind access panels) unless the
cabinet has been microbiologically decontaminated, is known to biologically clean, or known to be chemically inert.
Surface disinfection is performed as specified in the Cleaning Procedures section.
Hazardous Gases! Personal Protection Equipment Required.
! CAUTION
A disinfection using formaldehyde must be performed in accordance with the specifications of
NSF/ANSI 49, Annex G.
This procedure presents considerable risks and must be performed only by specially trained and authorized
service personnel in accordance with applicable national safety regulations.
The formaldehyde is vaporized within the tightly sealed sample chamber. The quantity of the applied
formaldehyde depends on the volume of the sample chamber in the safety cabinet that is to be disinfected.
The formaldehyde evaporates immediately after reaching its boiling point; the minimum reaction time is 6
hours. Therefore, the formaldehyde should be neutralized after the specified reaction time by vaporizing
ammonia.
Flammable Hazard!
! CAUTION
Formalin is flammable. The auto‐ignition temperature of formalin is 430 C (820° F).
With a volume percentage of 7.75% in dry air, formaldehyde vapor may explode.
For vaporization, do not use heating devices reaching temperatures above 250° C (477° F).
Chemical Hazard!
! CAUTION
Formalin in reaction with hydrogen chloride will form BCME which is a hazardous chemical.
When using formalin, all residues of hydrogen chloride in the work chamber of the cabinet must be
removed.
OM0222 Page 37 of 107
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The decontamination process is started by accessing the CABINET DECONTAMINATION on the main menu.
MENU
UV
FL
CALIBRATION/SERVICE UP
TIME/DATE
DOWN
PASSWORD
CABINET DECONTAMINATION ENTER
SCREEN/SET-UP
xx:xx AM xx/xx/xx
Select AUTO DECON‐FORMALIN, MANUAL DECON or AUTO DECON‐H2O2/CD. The Auto Decon‐Formalin process requires
the use of NuAire Accessories, NU‐984 Formalin/Ammonia vaporizer. The Auto Decon‐Formalin process may also require
the use of the NU‐912 Exhaust Decon Chamber if the cabinet is room re‐circulated or if the cabinet canopy is connected to
a facility exhaust system that restricts exposure to formaldehyde gas. The manual decon process requires the use of
NuAire accessory, Front and Top Seal Plates, (i.e. NU‐985‐301,401,501,601). The Auto Decon ‐ H2O2/CD requires the use of
NuAire accessory Auto Decon Exhaust Seal Plate (i.e. NU‐985‐302,402,502,602). Please contact NuAire Technical Service for
further assistance.
MENU
UV
FL
UP
AUTO DECON - FORMALIN
DOWN
MANUAL DECON
xx:xx AM xx/xx/xx
OM0222 Page 38 of 107
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7.1.1 Auto Decon‐Formalin
The auto decon formalin process is designed to decontaminate using liquid formaldehyde or formalin and liquid
ammonia. Each of these liquids will be placed in a vaporizer (NU‐984) located on the cabinets worktray. In
addition to the vaporizer, the exhaust decon chamber or canopy exhaust transition, if there are no facility exhaust
restrictions to formaldehyde gas, is used to seal the exhaust opening and provide a slight negative pressure on the
cabinet. The exhaust decon chamber is discharged back into the room via vapor collection cartridge. The canopy
exhaust transition would be directly vented into the facility exhaust system if allowed.
Note: Warning Label
A warning label should be attached to the safety cabinet during the vaporization process.
Formalin (37% Solution Added with Water, 1:1 Ratio)
On the basis of the calculated safety cabinet volume, use an industry standard acceptable formaldehyde quantity
that ensures an adequate formaldehyde concentration within the cabinet.
As stated above, the required formalin quantity depends on the cabinet volume that equals the industry standard
(1) of .30g/cu.ft. or 10.6g/cu.m of paraformaldehyde. In addition to the cabinet volume, to keep the cabinet at a
negative pressure during the decontamination process, a low volume of air is being exhausted either into the
vapor collection cartridge or if allowed, into the facility exhaust system. To assure the correct concentration of
formalin is maintained, an additional amount of formalin is added for the discharged volume of cabinet air.
Applying these amounts to readily available 37% formalin solution added with an equal amount of water will result
in the following requirements.
(1) NSF/ANSI 49, Annex G
NU‐480 Cabinet Volume Discharged Volume Total Volume Water/Formalin
Model Size cu.ft. (cu.m) cu.ft. (cu.m) cu.ft. (cu.m) Solution (37%)
(1:1 ratio = total (ml))
300 32.8 (.93) 5 (.14) 37.8 (1.07) 31:31 = 62
400 44.2 (1.25) 5 (.14) 49.2 (1.39) 40:40 = 80
500 55.7 (1.58) 5 (.14) 60.7 (1.72) 49:49 = 98
600 67.1 (1.90) 5 (.14) 72.1 (2.04) 58:58 = 116
Ammonia (50%)
The neutralization of formaldehyde gas is accomplished by vaporizing ammonia. Again, using the industry
standard (1) of 10% greater in mass ratio than paraformaldehyde/formalin and also accounting for the additional
discharge volume will result in the following requirements.
NU‐480 Liquid NU‐480 Liquid
Model Size ml Model Size ml
300 35 500 54
400 44 600 64
Auto Decon Run Cycle
An auto decon run cycle is already pre‐programmed into the BSCC system based on an 8 hour dwell time (hold
time). Other parameters such as formalin burn‐off formalin dwell (hold time) ammonia burn‐off, ammonia dwell
(hold time) and cabinet blower run time are also defaulted as shown below.
580
* Indicates when cabinet blower will run for default time of 30 seconds.
OM0222 Page 39 of 107
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Auto Decon‐Formalin Set Up
The default parameters can also be changed by accessing the auto decon set up parameter.
MENU
UV
FL
UP
AUTO DECON SET UP
DOWN
AUTO DECON RUN
OUTLET TEST
ENTER
xx:xx AM xx/xx/xx
Within the auto decon set up parameter, both gas cycle times and blower cycle times can be changed by following
the prompts.
Gas Cycle Times
MENU
UV
FL
UP
GAS CYCLE TIMES
DOWN
BLOWER CYCLE TIMES
ENTER
xx:xx AM xx/xx/xx
MENU
UV
FL
UP
FORMALIN BURN TIME
DOWN
FORMALIN GAS DWELL TIME
OM0222 Page 40 of 107
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MENU
UV
FL
UP
FORMALIN BURN TIME
20 MINUTES
DOWN
USE UP/DOWN TO CHANGE SETTING.
PRESS SAVE TO STORE THE NEW VALUE.
PRESS MENU TO EXIT. SAVE
xx:xx AM xx/xx/xx
Blower Cycle Times
MENU
UV
FL
UP
ENTER
xx:xx AM xx/xx/xx
MENU
UV
FL
UP
1st BLOWER TURN ON TIME
2nd BLOWER TURN ON TIME
3rd BLOWER TURN ON TIME DOWN
4th BLOWER TURN ON TIME
5th BLOWER TURN ON TIME
6th BLOWER TURN ON TIME ENTER
BLOWER RUN TIME
SET DECON BLOWER DUTY
xx:xx AM xx/xx/xx
OM0222 Page 41 of 107
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MENU
UV
FL
UP
1st BLOWER TURN ON TIME
10 MINUTES DOWN
USE UP/DOWN TO CHANGE SETTING
PRESS SAVE TO START THE NEW VALUE SAVE
PRESS SAVE TO EXIT
xx:xx AM xx/xx/xx
Decon blower duty cycle allows the blower to run at a lower speed when used during the decontamination
process.
MENU
UV
FL
UP
SET DECON BLOWER DUTY
XXX DUTY STEPS
DOWN
PRESS UP/DOWN TO CHANGE
SETTINGS. SAVE
PRESS SAVE TO SAVE THE NEW VALUE.
PRESS MENU TO EXIT.
xx:xx AM xx/xx/xx
Outlet Test
The outlet test can be performed to check outlet function for the decon process. Outlet #1 should be the left
outlet as you face the cabinet used for formalin and outlet #2 should be on the right used for ammonia.
MENU
UV
FL
UP
OUTLET #1
OUTLET #2
DOWN
OUTLET TEST
SELECT EITHER OUTLER TO TEST.
PRESS TOGGLE TO TEST EACH OUTLET ON AND OFF.
TOGGLE
xx:xx AM xx/xx/xx
OM0222 Page 42 of 107
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Auto Decon Run
To initiate the auto decon run cycle, select and enter auto decon run
MENU
UV
FL
UP
AUTO DECON SETUP
AUTO DECON RUN DOWN
ENTER
xx:xx AM xx/xx/xx
To prevent unauthorized entry into the auto decon cycle, the password must be entered.
The password would be the same as the blower password.
MENU
EXIT
0 5 UV
FL
1 6
ENTER PASSWORD BACK
2 7 XXXX
3 8 ENTER
4 9
xx:xx AM xx/xx/xx
Once the password has been entered, the procedure screen will appear.
MENU
UV
FL
1) INSTALL DECON EXHAUST CHAMBER.
2) REMOVE ARMREST.
3) PREPARE AND FILL FORMALIN VAPORIZER AND START
PLUG INTO THE LEFT OUTLET IN WORKZONE.
4) PREPARE AND FILL AMMONIA VAPORIZER AND
PLUG INTO THE RIGHT OUTLET IN WORKZONE.
5) LOWER WINDOW TO FULL DOWN POSITION.
6) APPLY WINDOW CLAMPS PROVIDED TO ASSURE
A TIGHT WINDOW SEAL.
7) PRESS DECON CYCLE START.
xx:xx AM xx/xx/xx
OM0222 Page 43 of 107
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Follow the procedure screen to make final preparations for the auto decon cycle. The decon exhaust chamber is installed
per ACD‐12009. Reference formalin and ammonia amounts as previously discussed. Once the cycle has started, it is
desirable to let the cycle run until finished. The display will indicate what portion of the cycle it’s in and the time
remaining. It is possible to abort the cycle by pressing the abort icon. A password screen will appear as verification that
the cycle abortion is valid. If pressed, the cycle will immediately go to the ammonium burn cycle followed by the
ammonium dwell. This is to assure, if any formaldehyde is present, it will be neutralized to mitigate any possible exposure
to formaldehyde gas.
Step 1: Install decon exhaust chamber
NOTE: If the cabinet is connected to an exhaust system via a canopy connection and it is acceptable to vent
formaldehyde into the facility exhaust system, then seal the canopy exhaust openings and reduce the exhaust flow
to hold the cabinet at a slight negative pressure (i.e. 0.1” w.g. (2.5mm w.g.) (24.82Pa)).
Proceed to step 2.
‐ Remove exhaust filter protector grill.
‐ Remove exhaust probe shroud.
‐ Attach and fasten the decon exhaust chamber over the exhaust HEPA filter with fasteners provided.
OM0222 Page 44 of 107
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‐ Attach decon chamber power plug to connector on top left of cabinet.
‐ Attach vapor collection cartridge to decon chamber tubing.
Step 2: Remove Armrest
OM0222 Page 45 of 107
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‐ Attach pressure gauge to drain valve with fittings provided.
Step 3: Prepare and fill formalin vaporizer and plug into the left outlet in vaporizer.
Step 4: Prepare and fill ammonia vaporizer and plug into the right outlet in workzone.
Step 5: Lower window to full down position.
Step 6: Apply window clamps provided to assure a tight window seal.
‐ Be sure window clamps are evenly spaced.
Turn knob until pressure pad comes in contact with the glass, then only tighten one full turn more.
OM0222 Page 46 of 107
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Note: DO NOT over tighten knobs or the safety plate glass could break.
Step 7: Press decon cycle start.
‐ Once the cycle is completed a message will appear indicating the cycle completion. Remove window clamps,
open window slowly and monitor for any residual vapors. Once clean, fully open window, remove vaporizer and
decon exhaust chamber. Replace exhaust sensor shroud and exhaust protective grill. Wipe down all workzone
surfaces to remove film generated by the decon process with a 10% ammonia/water solution.
OM0222 Page 47 of 107
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7.1.2 Manual Decon
1. Disconnect power to the cabinet. Remove screws at each upper side of the control center and allow the control
center to rotate down, resting on the safety straps. Disconnect electrical connectors on left side. Disconnect
electrical from right side. (Be sure to note the location of the supply and exhaust sensor wires before
disconnecting them from the main board). Loosen safety plate next to left hinge. Remove control center by
disconnecting safety straps and moving control center to the left off the slip hinges. Fold and pinch tubing to seal.
2. Remove the front decorative panel via top/front fasteners.
3. Remove window assembly (both frame and window, either being manual, sliding, or automatic) via the upper and
lower fasteners (Refer to BCD‐12281). At this point, the sliding window assembly can be removed.
4. Remove exhaust sensor shrouds.
5. Remove the diffuser screen and gasket around perimeter of workzone.
6. Place decontamination equipment inside the work area. Reference decontamination procedure,
per NSF/ANSI 49, Annex G, using the following chart to calculate chemical requirements.
Cabinet Size 300 400 500 600
Cabinet 58‐7/8 x 28 x 34‐3/8 in. 58‐7/8 x 28 x 46‐3/8 58‐7/8 x 28 x 58‐3/8 58‐7/8 x 28 x 70‐3/8
Dimensions (1.50 x .711 x .873 m) (1.50 x .711 x 1.18 m) (1.50 x .711 x 1.48m) (1.50 x .739 x 1.88 m)
Cabinet 32.79 cu. ft. 44.24 cu. ft. 55.69 cu. ft. 67.14 cu. ft.
Volume (.929 cu. m) (1.25 cu. m) (1.58 cu. m) (1.90 cu. m)
NOTE: The outlets in the work area are energized as long as the cabinet is plugged in and switched on the front panel.
Unplug the cabinet before decontamination equipment is plugged into these outlets. The control centers
electrical connectors may be re‐attached to utilize the cabinet's interior outlets and fan during the
decontamination. (See step 8)
8. Set control center on its side (with the bullet end down and the blower capacitor end up) on the left side of
cabinet. Then plug in the 16‐pin connector cable from the cabinet to the control center. Reconnect power to the
cabinet. The outlets can then be activated. The blower can be activated from the menu decon cycle.
9. Seal front and top openings using plastic and tape.
BE SURE CABINET IS TOTALLY SEALED TO PREVENT ANY
! CAUTION LABORATORY EXPOSURE TO DECONTAMINATION GAS.
OM0222 Page 48 of 107
March/2014
10. Perform decontamination procedure per NSF/ANSI 49, Annex G.
If the cabinet has been used to prepare antineoplastic drugs, (chemotherapy), or other toxic chemicals,
decontamination of the cabinet cannot be accomplished by the above procedure. It is recommended that the
following protective measures be taken:
1. Gloves
Gloves must be worn. Care must be taken not to cut, puncture, or tear the gloves. No one glove material
is impervious to all CYTAs; disposable surgical or polyvinyl chloride (PVC) gloves provide substantial but
not complete protection. PVC gloves probably are more protective than surgical gloves, but they are
stiffer and less tactile. Gloves should be discarded after each use. Gloves should be tucked into the cuffs
of the gown. Double gloving should be considered.
2. Face & Eye Protection
A disposable dust and mist respirator and either a plastic face shield (preferred) or chemical splash
goggles must be worn. The face shield or goggles should be wiped clean with a suitable tissue and water
after each use.
3. Gowns
A protective garment must be worn. The garment should be made of lint‐free, low‐permeability fabric
and must have a closed front, long sleeves, and elastic or knit closed cuffs. Tyvek¹ isolation gowns are one
example of an acceptable garment. The garment must be worn outside the work area. Disposable gowns
are preferred over reusable. Front‐buttoned coats are not recommended.
4. Hair & Shoe Covers
Disposable hair and shoe covers should be worn.
5. Motion
Slow and deliberate motions are necessary when working in the interior of the cabinet, in order to
minimize the generation of particulates.
Please consult with NuAire, Inc. about any unique contamination problems.
Normally, no preventive maintenance is required on the interior of the cabinet (i.e., the area behind the access
panel containing the HEPA filters and motor (blower assembly). All required adjustments in order to maintain
proper cabinet airflows are external to the cabinet interior. The motor is lubricated for life and is thermally
protected with automatic reset.
¹ Available from Lab Safety Supply, Janesville, WI 53547‐1368, or other laboratory, industrial, or hospital supply distributor
OM0222 Page 49 of 107
March/2014
7.1.3 Auto Decon ‐ H2O2/CD
The Auto Decon ‐ H2O2/CD process is intended to be used with any automated cabinet decontamination process
(i.e. hydrogen peroxide, chlorine dioxide, paraformaldehyde, etc.). The automated decon process parameters are
typically set up independently of the cabinet. Inlet and outlet ports from the automated decon machine are
connected to the cabinet. Typically the inlet is either connected to the drain valve fitting or an additional
connection point added to the cabinet. The outlet port is connected to a fitting on the auto decon exhaust seal
plate.
The instructions below indicate how to set up the cabinet to simultaneously run the cabinet blower at a low rate
during the decon cycle. This will aid in the process of distributing the decontaminant gas/vapor throughout the
cabinet during the entire decon cycle.
Auto Decon Set Up
Select AUTO DECON SET UP to enter the specific decon cycle parameters.
MENU
UV
FL
UP
AUTO DECON SET UP
AUTO DECON RUN DOWN
ENTER
xx:xx AM xx/xx/xx
Select DECON FULL CYCLE TIME
MENU
UV
FL
UP
1st BLOWER TURN ON TIME
2ND BLOWER TURN ON TIME
3RD BLOWER TURN ON TIME DOWN
4TH BLOWER TURN ON TIME
5TH BLOWER TURN ON TIME
6TH BLOWER TURN ON TIME ENTER
BLOWER RUN TIME
SET DECON BLOWER DUTY
DECON FULL CYCLE TIME
xx:xx AM xx/xx/xx
OM0222 Page 50 of 107
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Enter DECON FULL CYCLE TIME ranging from 30 to 720 minutes. This time should be the same as the automated
decon machine used for the process.
MENU
UV
FL
UP
DECON FULL CYCLE TIME
120 MINUTES DOWN
USE UP/DOWN TO CHANGE SETTING SAVE
PRESS SAVE TO START THE NEW VALUE
PRESS SAVE TO EXIT
xx:xx AM xx/xx/xx
Select and enter blower turn on times and length of blower run time at each interval as required by the automated
decon process used.
MENU
UV
FL
UP
1st BLOWER TURN ON TIME
10 MINUTES DOWN
USE UP/DOWN TO CHANGE SETTING
PRESS SAVE TO START THE NEW VALUE SAVE
PRESS SAVE TO EXIT
xx:xx AM xx/xx/xx
MENU
UV
FL
BLOWER RUN ON TIME UP
DOWN
USE UP/DOWN TO CHANGE SETTING
PRESS SAVE TO START THE NEW VALUE
PRESS SAVE TO EXIT SAVE
xx:xx AM xx/xx/xx
OM0222 Page 51 of 107
March/2014
Decon blower duty cycle allows the cabinet blower to run at a lower speed when used during the decontamination
process. The default setting has been determined as a minimum value, but adjustments can be made if necessary.
MENU
UV
FL
SET DECON BLOWER DUTY UP
XXX DUTY STEPS
DOWN
USE UP/DOWN TO CHANGE SETTING
SAVE
PRESS SAVE TO START THE NEW VALUE
PRESS SAVE TO EXIT
xx:xx AM xx/xx/xx
Auto Decon Run
To initiate the auto decon run cycle, select and enter ATUO DECON RUN.
MENU
UV
FL
UP
AUTO DECON SETUP
AUTO DECON RUN DOWN
ENTER
xx:xx AM xx/xx/xx
To prevent unauthorized entry into the auto decon cycle, the password must be entered.
The password would be the same as the blower password.
MENU
EXIT
0 5 UV
FL
1 6
ENTER PASSWORD
BACK
2 7 XXXX
3 8 ENTER
4 9
xx:xx AM xx/xx/xx
OM0222 Page 52 of 107
March/2014
Once the password has been entered, the procedure screen will appear.
MENU
UV FL
1) REMOVE ARMREST
2) INSTALL PLATE OR COVER OVER EXHAUST FILTER AREA
3) CONNECT DECON GAS INLET TO CABINET INLET GAS
FITTING ON TOP LEFT OF CABINET START
4) CONNECT DECON GAS RETURN TO CABINET RETURN
GAS FITTING ON EXHAUST PLATE
5) LOWER WINDOW TO FULL DOWN POSITION
6) APPLY WINDOW CLAMPS PROVIDED TO ASSURE
A TIGHT WINDOW SEAL
7) PRESS DECON CYCLE START
xx:xx AM xx/xx/xx
Follow the procedure screen to make final preparations for the auto decon H2O2/CD cycle. Once the cycle has
started, it is desirable to let the cycle run until finished. The display will indicate what portion of the cycle it’s in
and the time remaining. It is possible to abort the cycle by pressing the abort icon. A password screen will appear
as verification that the cycle abortion is valid. If pressed, the cycle will immediately reset to the start of cycle to
either re‐start or exit.
7.2 Fluorescent Lamp Bulb Replacement
The two (T8) fluorescent bulbs are cool white, capacitor start and placed external to the cabinet to aid maintenance and
minimize heat build‐up within the cabinet. The life rating of the bulb is 9000 hours based on three‐hour burning cycles.
To replace a bulb, it is necessary to remove the lamp assembly.
1. Switch Cabinet Light Switch off.
2. Remove the screws at each upper side of the Control Center and allow the Control Center to rotate down, resting
on the safety straps.
3. The bulb is now directly exposed for replacement.
4. The lamp bulb is removed by displacing the bulb to one side against the compressible bulb holder and lifting out
the lamp bulb.
5. Reverse the procedure to reinstall the lamp assembly being careful not to pinch the safety straps, cable or tubing
during closure of the control center.
7.3 HEPA Filter/Motor Replacement
The HEPA Filters under normal usage and barring an accident (a puncture), do not need replacement until the efflux
velocity cannot be maintained or the access inflow velocity cannot be maintained at 100 LFPM (.51 m/s) (min.). This may
permit the average downflow velocity to be as low as 55 LFPM (.27 m/s) as long as no point falls below 20% of the average
downflow velocity.
The HEPA Filters should not be replaced until the entire cabinet has been decontaminated or known to be biologically
"clean".
OM0222 Page 53 of 107
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7.3.1 Procedure
Disconnect electrical power from the cabinet before attempting any
! CAUTION
maintenance action.
Step 1: Remove screws at each upper side of the control center and allow the control center to rotate down,
resting on the safety straps. Second, remove the front decorative panel which is held into position by
(3) knurled nuts on the top edge and snap fit bullet catches on the bottom.
Step 2: Place sliding window into lowest position * and remove front filter panel, which is held into position by
Phillip pan head screws. Once the screws are removed, the panel is held into position by smooth weld
studs located on the top corner of the front filter panel. Use the window stop brackets as handles to
remove the panel.
NOTE: Screws are used in lieu of acorn nuts, and lock washers.
The screws have O‐rings and should be replaced if damaged or badly deformed.
The interior of the cabinet is now fully exposed for replacement of the filters and/or motor/blower.
*The lowest window position will require the armrest to be removed. If the cabinet has the power window option,
access the Power Window Test menu to adjust the power window into its lowest position with the armrest
removed. Access Calibration/Service menu, then Service menu, then Power Window menu to Power Window
Test.
Step 3: Filter Removal
It is not always necessary to replace both the supply and exhaust filters at the same time. If during the
course of certifications, the downflow always falls off while the exhaust increases the supply filter is
"loading" faster than the exhaust filter and only the supply filter may need replacement. The opposite
might also happen depending upon many factors.
a. To remove the supply filter:
1. Remove the plenum screws in front of the supply HEPA filter.
2. Lift the permanent plenum and hold up with wire strap.
3. Carefully remove the supply filter. Direct exposure should be avoided.
Dispose of spent HEPA filters properly.
Avoid direct contact to "dirty side" of the filters.
!
CAUTION
Place in sealed bag and label all waste containers/cartons based on
type of hazard. Follow all Local, State and Federal guidelines for
disposal of HEPA filter solid waste.
b. To remove the Exhaust HEPA:
1. Relax the exhaust filter seal loading mechanism by turning the four threaded bolts
counterclockwise until one can see a definite release of the loading springs.
2. Pull the exhaust choke tray free and remove the filter. It is not necessary to remove the
tray, although it is free to move forward several inches, if necessary, to free the HEPA filter.
OM0222 Page 54 of 107
March/2014
Step 4: Filter Installation
When installing new filters, USE ONLY NUAIRE SPECFIED FILTERS FOR REPLACEMENT.
Description: Supply HEPA Filter Exhaust HEPA Filter
Efficiency: 99.995% @ 0.3 Micron 99.995% @ 0.3 Micron
Airflow Rating: 100 fpm @ .60 .05" w.g. per sq. ft. 250 fpm @ .70 .05" w.g. per sq. ft.
Frame Type: Metal Metal
NU‐480‐300
NuAire Part A‐980962‐01 A‐980962‐05
Number:
Filter Size: 21" (533mm) x 32" (813mm) x 3" (76mm) 20" (508mm) x 14" (366mm) x 11 1/2"
(292mm)
Filter Manufacturer: Camfil Farr Camfil Farr
NU‐480‐400
NuAire Part A‐9809957‐06 A‐9809957‐09
Number:
Filter Size: 21" (533mm) x 44" (1118mm) x 3" 20" (508mm) x 20” (508mm) x 11 1/2"
(76mm) (292mm)
Filter Manufacturer: Camfil Farr Camfil Farr
NU‐480‐500
NuAire Part A‐9809957‐07 A‐9809957‐10
Number:
Filter Size: 21" (533mm) x 56" (1422mm) x 3" 20" (508mm) x 26" (660mm) x 11 1/2"
(76mm) (292mm)
Filter Manufacturer: Camfil Farr Camfil Farr
NU‐480‐600
NuAire Part A‐9809957‐08 A‐9809957‐11
Number:
Filter Size: 21" (533mm) x 68" (1727mm) x 3" 20" (508mm) x 32" (813mm) x 11 1/2"
(76mm) (292mm)
Filter Manufacturer: Camfil Farr Camfil Farr
a. To Install the supply filter, simply reverse the procedure outlines in Step 3a, above.
Note: Be sure to open the choke plate fully before inserting the filter into the tray.
This will assist in adjusting the airflow.
b. To install the exhaust filter, apply a thin layer of silicone grease to the top and bottom gaskets of the
filter and carefully insert into the exhaust choke tray.
Position the filter frame within the outside walls of the exhaust opening on the top of the hood.
Tighten the spring loaded bolts, 4 places, depressing the gasket material by 1/8 inch (3mm)
Step 5: Motor/Blower Assembly Removal
a. It is recommended that the motor/blower to be removed as a single unit. To remove, disconnect
electrical connections to the motor, remove the HEPEX pressure plenum and unbolt the
motor/blower assembly from the roof of the cabinet (4 places). Always inspect the rubber isolation
motor mounts and replace those that are cracked or visibly show stress.
b. Replace the motor exactly as originally installed in the blower housing, paying particular attention to
the correct electrical connections (see Electrical Schematic).
c. Re‐install the new motor/blower assembly.
OM0222 Page 55 of 107
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7.4 Sliding Window Replacement & Manual Adjustment
The sliding window replacement is accomplished by removing the front decorative panel, control center, and window glide
assemblies. The sliding window adjustment may be required due to everyday use over the life of the cabinet. Both the
right and left window glides are adjustable by a set screw and tension screw method. When adjusting the sliding window,
be sure to verify proper micro switch operation. If the sliding window is too loose, the sliding window will not properly
activate the micro switches, thus causing potential operational malfunctions to occur. In addition, the sliding window
retention or ability to slow the rate of fall, if a window counter balance experiences a fault, is also required to assure proper
window function (see Sliding Window Retention Verification in the Inspection Report).
7.5 Airflow Control System Setup and Calibration
7.5.1 General
The operation of the NU‐480 cabinet requires that the setup and calibration procedures be performed in order to
certify or commission the cabinet for usage. The setup and calibration procedures performed ONLY BY THE
CABINET CERTIFIER ensure that cabinet's setpoints are verified and that the airflow monitor sensors are calibrated
to read the correct values. Press MENU to access Calibration/Service parameter.
MENU
UV
FL
CALIBRATION/SERVICE
DOWN
TIME/DATE
PASSWORD
ENTER
CABINET DECONTAMINATION
SCREEN SET-UP
FILTER STATUS
xx:xx AM xx/xx/xx
Entry into the Calibration/Service functions requires a service password for entry. After pressing the
Calibration/Service menu item, a password screen will appear. The default password is “9876”.
Once the service password is entered, the Calibration/Service menu will appear.
EXIT
MENU
UV
0 5 FL
1 6
ENTER PASSWORD
XXXX BACK
2 7
3 8 ENTER
4 9
xx:xx AM xx/xx/xx
OM0222 Page 56 of 107
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7.5.2 Calibration/Service Menu
The Calibration/Service menu provides a list of sub‐menu items to accomplish all service tasks. For airflow
calibration, only the first three sub‐menu items are used in the calibration process.
MENU
UV
FL
SETPOINTS/LIMITS DOWN
SENSOR SETUP
NIGHT SETBACK
SERVICE ENTER
OPTION SETUP
DIAGNOSTICS
CABINET RESET
CABINET TYPE
xx:xx AM xx/xx/xx
7.5.2.1 Cabinet Type
The cabinet type can be verified in the control system and is factory set and shouldn’t require alteration.
The cabinet type default information controls unit of measure, setpoints and limits based on the type and
size of cabinet.
To verify, press CABINET TYPE. The current type of cabinet will be designated. Again to verify, press the
correct Type and the Model/Size of the current selected cabinet model will be designated. Once verified
the correct type and model are designated, then press MENU to return to Calibration/Service menu.
CURRENTLY A2 NU-480-300/400/500/600 MENU
UP
TYPE A2 (70% RECIRCULATE/
UV 30% EXHAUST)
FL
TYPE B1 (30% RECIRCULATE/ 70%EXHAUST)
DOWN
TYPE B2 (100% EXHAUST)
ENTER
SET OEM DISPLAYED
SET MOTOR TYPE
xx:xx AM xx/xx/xx
CURRENTLY A2 NU-480-300/400/500/600 MENU
UP
A2 NU-440-300/400/500/600
A2 NU-440-300/400/500/600(E) DOWN
A2 NU-440-300/400/600(D
A2 NU-480-300/400/500/600
A2 NU-480-300/400/500/600(E) SAVE
A2 NU-480-300/400/600(D)
xx:xx AM xx/xx/xx
OM0222 Page 57 of 107
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7.5.2.2 Setpoints/Limits
The airflow setpoints and alarm limits may also be verified or altered. Typically these default values are
factory set based on the cabinet type, model and size as previously discussed. However, they may be altered
in special cases for modified cabinets. The setpoint establishes the airflow values that are to be maintained.
The high low limits establish the alarm boundaries from the nominal setpoint. The default values have been
established based upon the performance specifications and cabinet component tolerances.
To verify or alter any of the airflow setpoints or alarm limits, press the menu setpoints/limits menu item.
Then, press any of the individual setpoints or alarm limits to verify and/or change. Press UP or DOWN to
change new value. Press SAVE to enter new value.
MENU
UV FL
DOWNFLOW SETPOINT
UP
DOWNFLOW HIGH LIMIT
DOWNFLOW LOW LIMIT DOWN
EXHAUST FLOW SETPOINT
ENTER
EXHAUST FLOW HIGH LIMIT
EXHAUST FLOW LOW LIMIT
xx:xx AM xx/xx/xx
MENU
UV
FL
UP
OLD DOWNFLOW SETPOINT 60 fpm
NEW DOWNFLOW SETPOINT 60 fpm
DOWN
USE UP/DOWN TO CHANGE NEW DOWNFLOW
SETPOINT. PRESS ENTER TO SAVE NEW VALUE. SAVE
PRESS MENU TO EXIT WITHOUT CHANGE
xx:xx AM xx/xx/xx
Default values for NU‐480
Downflow setpoint ‐ 60
Downflow high limit ‐ 66
Downflow low limit ‐ 54
Inflow setpoint ‐ 105
Inflow high limit ‐ 120
Inflow low limit ‐ 90
OM0222 Page 58 of 107
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7.5.3 Airflow Calibration
The NU‐480 airflow calibration consists of adjustments to balance the airflow within the cabinet and the
calibration of the airflow monitor sensors. THIS WORK SHOULD BE DONE ONLY BY A QUALIFIED TECHNICIAN
WHO CAN MEASURE THE AIRFLOW FROM THE FILTERS WITH A SUITABLE VELOMETER. NuAire provides two
adjustments to balance the airflow within the cabinet. These are:
a. PWM signal adjust via DC ECM motor control system
b. Exhaust filter choke
The blower speed control system adjusts the cabinet's total volume of airflow while the choke adjusts or balances
the exhaust airflow as well as makes up for filter resistance tolerances. Since it has been NuAire's experience that
the filters may not "load" evenly, both adjustments are necessary for proper cabinet performance.
The cabinet is considered to be certifiable if the following airflow measurements are present:
a. Downflow average: 60 LFPM 5 LFPM (.30 m/s .025 m/s).
b. Inflow average: 105 LFPM 5 LFPM (.53 m/s .025 m/s) using the
Direct Inflow Measurement (DIM) method, or
alternate 3” constricted inflow velocity measurement method.
The calibration of the airflow monitor sensors occurs during the cabinet airflow balancing procedure. The
calibration procedure consists using the downflow and inflow averages achieved and entry of those values into the
control system.
DC ECM motor PWM signal DC voltage should also be monitored and recorded upon final calibration. The DC
voltage may be monitored using an independent digital voltmeter in the VDC mode. The two test points to
measure DC ECM motor voltage are located on the DC motor signal connector on the main control board.
MOTOR VOLTAGE TEST POINTS
BEFORE STARTING AIRFLOW CALIBRATION PROCEDURE,
LET THE CABINET RUN FOR AT LEAST 10 MINUTES.
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7.5.3.1 Downflow Calibration
Step 1: Access Calibration/Service menu.
Press SENSOR SETUP menu item to access sensor calibration menu.
MENU
UV
FL
UP
CALIBRATE DOWNFLOW SENSOR
CALIBRATE EXHAUST SENSOR DOWN
FIND NEW DOWNFLOW SENSOR
FIND NEW EXHAUST SENSOR ENTER
xx:xx AM xx/xx/xx
Step 2: Press CALIBRATE DOWNFLOW SENSOR to access individual calibration screen.
Press BLOWER switch to on. Allow blower to run for one minute or until downflow readings
are steady.
UV
MENU
FL
MOTOR RPM = XXXX
BLOWER DUTY CYCLE = XX.XXX UP
RAW DOWNFLOW = XXX fpm DOWN
CALIBRATED DOWNFLOW = XXX fpm
USE UP/DOWN TO CHANGE CALIBRATED SAVE
DOWNFLOW, PRESS ENTER TO SAVE NEW VALUE
PRESS BLOWER DUTY CYCLE TO CHANGE IT
PRESS
MENU TO EXIT WITHOUT CHANGE
xx:xx AM xx/xx/xx
Step 3: Place a velometer in the cabinet workzone on the horizontal plane 4 inches (102mm)
above the bottom edge of the window.
Spot check several points on the recommended downflow velocity test grid found in table 7.0.
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Step 4: Press BLOWER DUTY CYCLE to adjust blower speed.
The objective of this spot check is to obtain the desired downflow average velocity as close
as possible to the stated goal of 60 LFPM (.30 m/s)
MENU
UV
FL
UP
MOTOR RPM = XXXX
DOWN
BLOWER DUTY CYCLE = XX.XXX
SAVE
PRESS UP/DOWN TO CHANGE BLOWER DUTY CYCLE.
PRESS MENU TO EXIT WITHOUT CHANGE.
xx:xx AM xx/xx/xx
DON'T SPEND MORE THAN 5 MINUTES SPOT CHECKING.
FINAL ADJUSTMENTS WILL BE MADE IN THE FOLLOWING STEPS.
Press SAVE to enter the current blower speed.
Step 5: Now, measure the average downflow velocity over the entire workzone using the recommended
downflow velocity test grid (see Table 7.0).
Step 6: Press UP or DOWN arrows to change the calibrated downflow value to the average downflow
velocity just found.
Press SAVE to enter the new calibrated downflow value.
Now the downflow monitor sensor has been calibrated to the actual measured average
downflow velocity. The cabinet will now control to the downflow setpoint.
Step 7: Press MENU to exit back to the sensor calibration menu and blower will be turned off
automatically.
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7.5.3.2 Inflow Calibration
NOTE: INFLOW CALIBRATION MUST BE MADE IMMEDIATELY FOLLOWING DOWNFLOW CALIBRATION. THIS
ASSURES THE CORRECT BALANCE OF DOWNFLOW TO INFLOW AS RELATED TO THE AIRFLOW MONITOR
SENSOR CALIBRATIONS.
Step 1: Access sensor setup menu.
MENU
UV
FL
UP
CALIBRATE DOWNFLOW SENSOR
CALIBRATE EXHAUST SENSOR DOWN
FIND NEW DOWNFLOW
SENSOR
ENTER
FIND NEW EXHAUST SENSOR
xx:xx AM xx/xx/xx
Step 2: Press CALIBRATE EXHAUST SENSOR to access individual sensor calibration screen.
Press blower switch on.
MENU
UV
FL
DOWNFLOW = X.XX mps SETPOINT = X.XX mps UP
RAW EXHAUST = XXX fpm DOWN
CALIBRATED EXHAUST = XXX fpm
SAVE
USE UP/DOWN TO CHANGE CALIBRATED
EXHAUST, ENTER TO SAVE NEW VALUE
PRESS DOWNFLOW SETPOINT TO CHANGE IT
PRESS MENU TO EXIT WITHOUT CHANGE
xx:xx AM xx/xx/xx
Step 3: Now measure the inflow velocity using the recommended procedure found in Table 7.0.
If necessary, adjust the exhaust filter choke located under the front decorative panel, to achieve
the correct average inflow velocity within the stated range of 105 5 LFPM (.53 .025 m/s).
IF THE AVERAGE INFLOW VELOCITY IS ON THE OUTER EDGE OF THE RANGE (I.E. 100 or 110 fpm
(.51 or .56m/s)). IT WOULD BE HIGHLY DESIRABLE TO MOVE IT CLOSER TO THE SETPOINT
BECAUSE THE INFLOW MONITOR SENSOR ONLY FOLLOWS THE EXHAUST AIRFLOW OF THE
BLOWER SYSTEM. There is no automatic exhaust control for inflow to setpoint via an actuator.
However as a defaulted “ON” option, there is an Exhaust Auto CAL function that will periodically
adjust the inflow display to setpoint. Having the inflow calibrated to the nominal setpoint will
allow the system to run efficiently and accurately.
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NOTE: The choke plate adjustment requires standard blade screwdriver. To adjust, loosen the liquid‐
tight fitting around the choke adjustment shaft. While monitoring the exhaust flow to check
position, turning the choke adjustment shaft clockwise will open the choke while turning counter
clockwise closes the choke.
Step 4: Now the calibration procedure is complete. Return to the Run Mode using the menu icon to
back out of the Calibration/Service menu. Press blower switch to initiate the warm‐up cycle.
Allow the blower to go through the entire warm‐up cycle so the Exhaust Auto CAL process can
take place at the end of the warm‐up cycle.
NOTE: If the Exhaust Auto CAL optional function is turned off the offset calibration must be performed
when you are still in the Calibrate Exhaust Sensor menu.
Press UP or DOWN to change the calibrated exhaust value to the average inflow velocity just
found.
Press SAVE to enter the new calibrated inflow value.
Now, the calibration procedure is complete. If desired, a spot check in the downflow velocity
may be performed if felt necessary. Replace the exhaust HEPA filter protective grill, if removed.
If the inflow display changes due to the protective grill being present. Re‐enter into Calibrate
Exhaust screen and correct calibrated exhaust value.
Once entire cabinet has been balanced, tighten liquid tight fasteners around choke adjustment
shaft.
OM0222 Page 63 of 107
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Table 7.0
Recommended Measurement Methods for Cabinet Downflow & Inflow
A. Downflow Measurement
a. Recommended Instruments: TSI 8355 Thermo anemometer
b. Procedure: Supply filter efflux is measured on a grid, in a horizontal plane 4 inches (102mm) above the bottom
edge of the window. No reading should be taken closer than 6 inches (152mm) from the inside perimeter.
c. Test Data ‐ Inches (mm):
(Access Opening)
8” 10”
6 11.594 17.188 22.782 28.375
300 300
(152) (295) (437) (597) (721)
6 11.729 17.458 23.187 28.916 34.645 40.375
400 400
(152) (298) (443) (589) (735) (880) (1026)
6 11.797 17.594 23.391 29.188 34.985 40.782 46.579 52.375
500 500
(152) (300) (447) (594) (741) (889) (1036) (1183) (1330)
6 11.838 17.676 23.514 29.352 35.190 41.028 46.866 52.704 58.542 64.375
600 600
(152) (301) (449) (597) (746) (894) (1042) (1190) (1339) (1487) (1635)
6 6
(152) (152)
10.540 10.460
(268) (266)
15.080 14.920
(393) (379)
19.620 19.380
(498) (492)
Number of Readings: Average Velocity ft./min.(m/s)
d. Acceptance Criteria:
1. Average downflow velocity = 55 to 65 fpm (.28 to .33 m/s)
2. Individual readings must be within + 20% or + 16fpm (+ 0.08m/s) whichever is greater (factory test) or
+ 25% or + 16fpm (+ 0.08m/s) whichever is greater (field test) from the average downflow velocity.
B. Inflow Measurement
a. Recommended Instrument: Shortridge Flowhood ADM‐870 or TSI 8355 Thermo anemometer.
b. Primary Procedure:
The primary procedure to determine inflow velocity uses a Direct Inflow Measurement (DIM) Instrument (i.e.
Shortridge flowhood). The DIM Instrument can be used directly on the cabinet with NO CORRECTION FACTORS
REQUIRED if operated in the local density default mode. NSF has tested the cabinet and established listed air
velocities expressed in local density. The DIM Instrument should be equipped with a flowhood that is as close as
possible to the width of the cabinet (i.e. NU‐480‐400 should use 1 x 4 foot flowhood). The DIM Instrument should
also be duct taped to the cabinet to prevent any sneak air paths from occurring.
The DIM Instrument will read inflow volume (i.e. CFM). Use the window access opening area to calculate inflow
velocity.
OM0222 Page 64 of 107
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Alternate Procedure:
The alternative procedure to determine inflow velocity uses a thermo anemometer in a constricted window access
opening of 3 inches (76mm) with the armrest removed. Inflow air velocity is measured in the center of the
constricted opening 1‐1/2 inches (38mm) above the work access opening on the following specified grid. Use the
correction factor table to calculate the inflow velocity.
c. Test Data ‐ Inches (mm):
1. DIM Measurement
Inflow Volume t.3/min.(m3/s) Access Opening ft.2(m2) Inflow Velocity ft./min(m/s)
2. Constricted 3 inch (76mm) high access opening measurement ‐ Inches (mm):
4 8.3966 12.792 17.188 21.584 25.980 30.375
300
(102) (213) (325) (437) (548) (660) (771)
4 8.264 12.528 16.792 21.056 25.320 29.584 33.848 38.112 42.375
400
(102) (210) (318) (426) (535) (643) (751) (860) (968) (1076)
4 8.198 12.396 16.594 20.792 24.990 29.188 33.386 37.584 41.782 45.980 50.178 54.375
500
(102) (208) (315) (421) (528) (635) (741) (848) (955) (1061) (1168) (1274) (1381)
4 8.158 12.316 16.474 20.632 24.790 28.948 33.106 37.264 41.422 45.580 49.738 53.896 58.054 62.212 66.375
600
(102) (207) (313) (481) (524) (630) (735) (841) (946) (1052) (1158) (1263) (1369) (1475) (1580) (1686)
Number of Readings: Average Velocity of Constricted Area ft./min.(ms)
Average Velocity fpm (mps) X Constricted Area ft2 (m2) = Constricted CFM (m3/s)
of Constricted Area Area Volume
Constricted Area Volume CFM (m3/s) 8" (203mm) ft2 (m2) = Average Velocity fpm (mps)
Access Window Area of 8" (203mm)
Access Window Area
Average Velocity of 8" (203mm) fpm (mps) X Correction Factor =Average Inflow Velocity fpm (mps)
Access Window Area
d. Acceptance Criteria: Access Opening Inflow Velocity = 100 to 110 fpm (.51 to .56 m/s)
Areas/Correction Factors for Calculations
Cabinet 3" (76mm) 8" (203mm) 10" (254mm) Correction Factor for Correction Factor for
Size Constricted Window Window Access Window Access 8" (203mm) 10" (254mm)
Access Area Opening Opening Window Window
300 .72 1.91
‐ 1.22 ‐
(.067) (.177)
400 .97 2.58 3.22
1.22 1.21
(.090) (.240) (.299)
500 1.22 3.24 4.05
1.22 1.20
(.113) (.301) (.376)
600 1.47 3.91 4.89
1.18 1.15
(.137) (.363) (.454)
OM0222 Page 65 of 107
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7.6 HEPA Filter Leak Test
In order to check filter and filter seal integrity, the HEPA filter media and seals must be directly accessible, by the measuring
instrument. The challenge material (i.e. PAO) should be supplied in the rear center of the workzone over the intake slots.
The upstream challenge port being common for both filters in located on top of the cabinet.
7.6.1 Supply Filter
The diffuser plate placed below the HEPA to protect the filter during normal usage may be removed as follows:
The diffuser is secured to the cabinet shell by #1/4‐20 acorn nuts located immediately behind the front viewing
window. After removing the fasteners, drop the front of the diffuser plate several inches and pull forward gently.
Note that the diffuser is purposely a tight fit ‐ it is held to the back wall of the cabinet interior by a light push ‐ fit
with projecting studs. To avoid the window high alarm during the filter integrity check. It is desirable to enter the
Calibration/Service menu and turn on the blower.
Air currents in the room can sometimes contribute to exhaust entrainment on top of the cabinet. This can lead to
false leak readings. In order to reduce the probability of false leak readings it is recommended to cover half of the
exhaust HEPA filter while scanning the uncovered section of the exhaust HEPA filter.
7.6.2 Exhaust Filter
The exhaust filter is typically more difficult to check since protective grills, charcoal filters, or exhaust transitions
could cover the filter. Access panels are usually provided and should be removed. If an air gap exhaust transition
is provided, the air gap must be sealed with duct tape or other suitable means to prevent contaminated air from
migrating into the exhaust efflux. All exhaust blowers/fans should be turned off during the check. The exhaust
sensor shroud can also be removed for the exhaust filter integrity check. Replace the exhaust sensor shroud
immediately after the filter check to assure proper operation.
NOTE: To avoid the window high alarm during the filter integrity check, it is desirable to enter into the
Calibration/Service menu and turn the blower on. To accomplish this, perform the following:
Select Calibration/Service
MENU
UV
FL
UP
CALIBRATION/SERVICE
TIME/DATE DOWN
PASSWORD
CABINET DECONTAMINATION ENTER
SCREEN SETUP
FILTER STATUS
xx:xx AM xx/xx/xx
OM0222 Page 66 of 107
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Entry into the Calibration/Service functions requires a service password for entry. After pressing
Calibration/Service menu item, a password screen will appear. The default password is “9876”.
Once the service password is entered the Calibration/Service menu will appear.
0 5 MENU
EXIT
1 6
ENTER PASSWORD
BACK
XXXX
2 7
3 8 ENTER
4 9
xx:xx AM xx/xx/xx
NOTE: If the upstream challenge port is deemed contaminated and not accessible, use both downflow and exhaust
volume for determining challenge concentrations. Use following area information below with average
downflow velocity and spot‐check exhaust velocities as measured to determine volume (CFM) (CMH).
Model Size *Supply Area (ft2)(m2) Exhaust Area (ft2)(m2)
300 6.33 (.588) 1.61 (.150)
400 8.53 (.792) 2.38 (.221)
500 10.74 (.998) 3.15 (.293)
600 12.95 (1.203) 3.92 (.364)
* Measured 4 inches above the bottom edge of the window.
Laskin Nozzle Concentration Formula
# Nozzles x 135 CFM x 100 ug/L Challenge
=
Downflow (CFM) + Exhaust (CFM) Concentration (ug/L)
# Nozzles x 229 CMH x 100 ug/L = Challenge
Downflow (CMH) + Exhaust (CFM) Concentration (ug/L)
7.7 Airflow Smoke Pattern Test
The airflow smoke pattern test is performed using a smoke source (i.e. smoke tubes) in and around the cabinet workzone
and access opening to determine a visual representation of the cabinet’s containment performance. To perform the test,
the smoke source should be passed through the following areas:
1. A smoke source shall be passed from one end of the cabinet to the other, along the center line of the work
surface, at a height of 4 inches (102mm) above the top of the access opening.
2. A smoke source shall be passed from one end of the cabinet to the other, 1 inch (25mm) just inside the view
screen, at a height 6 inches (152mm) above the top of the access opening.
3. Pass a smoke source along the edges of the entire perimeter of the work opening approximately 1.5 inches
(38mm) outside the cabinet, with particular attention paid to corners and vertical edges.
4. Pass a smoke source 2 inches (51mm) from the sides up inside of the window at the side channel seals, and along
inside of the cabinet along the top of the work area or immediately below the wiper gasket.
The criteria used to evaluate the smoke patterns is the following:
1. The smoke inside the cabinet shall show smooth downward flow with no dead spots or reflux.
2. No smoke shall escape from inside the cabinet.
3. No smoke refluxes out of the cabinet once drawn in, nor does smoke billow over the worksurface or penetrate
onto it.
4. No smoke shall escape from the cabinet.
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7.8 Cleanliness Classification Test for Pharmacy Application
If this cabinet is going to be used within pharmacy, per USP7971, the cabinet must be tested to assure compliance to ISO
14644‐1:1999, Cleanrooms and Associated Controlled Environments, Part 1: Classification of Air Cleanliness2. The
cleanliness classification test is performed using a particle counter to measure particle counts within the cabinet workzone.
Turn on cabinet and let warm up for several minutes. Turn on particle counter and flush out sample tubing line to remove
latent particles. Set the particle counter to measure 0.5 micron or larger particles at the appropriate measuring rate.
“Operational Particle Count Test3”
Position the particle counter isokinetic probe at a point 6 inches (152mm) upstream of the aseptic manipulation area (hand
convergence point) and mounted so as not to interfere with the operator’s hand movement. The pharmacy operator will
simulate IV manipulation during the particle count test using non‐hazardous materials. A minimum of three (3) 1‐minute
particle counts shall be sampled and recorded while the user simulates aseptic compounding manipulations.
“At Rest Particle Count Test”
Take 5 test points in 1‐minute intervals on a grid, in a horizontal plane as measured approximately 6 inches (152mm) above
the worksurface. The grid location is designed as the workzone center point and each corner measured 6 inches (152mm)
from the inside perimeter.
Record the 5 particle count values for each of the test points over the 1‐minute sample time. All final count particle
concentrations and calculated 95% upper confidence limit shall not exceed 3520 particles per cubic meter (ppcm) or (100
particles per cubic feet (ppcf).
1
USP28‐NF23: United Stated Pharmacopeial Convention, Inc., 12601 Twinbrook Parkway, Rockville, MD 20852, USA, www.usp.org.
2
ISO 14644‐1:1999 Cleanrooms and Associated Controlled Environments‐Classification of Air Cleanliness, International Organization for Standardization,
Case Postale 56, CH‐1211 Geneve 20, Switzerland
3
CAG‐002‐2006: CETA Compounding Isolator Testing Guide, Controlled Environment Testing Association, 1500 Sunday Drive, Suite 102, Raleigh, NC
27607, USA, www.cetainternational.org
OM0222 Page 68 of 107
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7.9 Main Control Board Description & Replacement
To access the main control board for fuse or board replacement, remove screws at each upper side of the control center
and allow the control center to rotate down, resting on the safety straps. Now the main control board is exposed for
service.
7.9.1 Main Control Board Replacement
The main control board consists of two interconnected Printed Circuit Board (PCB) assemblies. The front PCB
contains the LCD display. The back PCB contains the power supply, configuration switch, sensor inputs/outputs
and control inputs/outputs components. The mechanical and electrical interconnects for the two PCB's all occur
within the assemblies and are fastened together with standoffs and screws.
7.9.2 Main Control Board Fuse Replacement
! CAUTION Disconnect electrical power from cabinet before fuse replacement.
All AC circuits are fuse protected and when replacement is necessary, USE ONLY FUSES OF SAME TYPE AND RATING FOR
PROTECTION AGAINST RISK OF FIRE.
ACCESSORY POWER
DESCRIPTION: BLOWER FUSE OUTLET FUSE LIGHT FUSE
OUTPUT FUSE INPUT FUSE
FUSE TYPE: TIME‐LAG TIME‐LAG TIME‐LAG TIME‐LAG SUB‐MIN (250V)
Disconnect electrical power from the cabinet before attempting any
! CAUTION
maintenance action.
The main control board is fastened to the control center with (6) 6‐32 screws. All electrical connections are made
with removable terminals and/or Faston connectors except for the motor/blower connector which uses a screw
terminal. Remove all electrical connections and fasteners then remove the main control board from the control
center.
Install new main control board by reattaching all electrical connections and fasteners. Once installed, rotate
control center to normal position and fasten in place.
Now reconnect power to cabinet. Upon BSCC system power up, a system master reset must be performed to clear
the microprocessors non‐volatile memory to assure proper system function.
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7.9.4 Cabinet Reset
The main control board has two software operating resets available for qualified service personnel. The two types
are the following:
Factory Reset ‐ Resets setpoints and selected option settings. Factory reset should be used in the event the
system memory develops an error in operation. Cabinet type, motor type, and calibration
data will not be affected with this reset.
Master Reset ‐ Resets all calibration, cabinet type, motor type sensor data, and options settings back to
default settings. Master reset should only need to be used for a main control board
replacement.
After pressing the Calibration/Service menu item, a password screen will appear. The default password is “9876”.
Once the service password is entered, the Calibration/Service menu will appear.
MENU
0 5 UV EXIT
FL
1 6
2 7 ENTER PASSWORD BACK
XXXX
3 8
ENTER
4 9
xx:xx AM xx/xx/xx
Select CABINET RESET from the menu.
MENU
UV
FL
UP
SETPOINTS/LIMITS
SENSOR SETUP
NIGHT SETBACK
DOWN
SERVICE
OPTION SETUP
DIAGNOSTICS
ENTER
CABINET RESET
CABINET TYPE
xx:xx AM xx/xx/xx
OM0222 Page 70 of 107
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Select desired function from menu.
MENU
UV
FL
UP
FACTORY RESET DOWN
MASTER RESET
ENTER
xx:xx AM xx/xx/xx
Perform either reset function as selected below.
MENU
UV
FL
FACTORY RESET
RESET
PRESS RESET TO INITIATE A FACTORY RESET.
THIS WILL RESET THE CABINET BACK TO ITS
FACTORY DEFAULT SETTINGS.
PRESS MENU TO EXIT WITHOUT CHANGE.
xx:xx AM xx/xx/xx
Once factory reset is complete, return to Calibration/Service menu to enter any options.
MENU
UV
FL
MASTER RESET
RESE
PRESS RESET TO INITIATE A MASTER RESET. THIS WILL
CAUSE ALL CALIBRATION DATA AND USER SETTINGS
TO BE LOST!!
PRESS MENU TO EXIT WITHOUT CHANGE.
xx:xx AM xx/xx/xx
Once the MASTER RESET icon is pressed, the display screen will remain the same for approximately 1 minute.
Also during this 1 minute, an audible signal of the reset process will occur. Once the reset process is complete the
display screen will revert back to the NuAire logo main menu. At this point the cabinet MUST be turned off to
complete the process. Either unplug the cabinet or use the power switch within the control center to turn off the
cabinet. Turn the cabinet back on the display screen will remain blank for up to a minute, then will indicate
“Power Loss Alert”, press the screen to clear the message and return to the Calibration/Service menu to enter
cabinet type, motor type, find sensors and perform airflow calibration.
OM0222 Page 71 of 107
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7.10 Digital Airflow Sensor Description & Replacement
The airflow sensor function utilizes two thermistors that provide a constant current source. One thermistor is a
reference that uses a very low current source. The other thermistor is the airflow measurer that uses a very high
current source. As airflow passes over the thermistors, the airflow removes heat from the thermistor measuring
airflow. The loss of heat from the thermistor causes the voltage from the thermistor to increase. This increase
subtracted from the reference thermistor output voltage is what directly relates to airflow velocity. A repeatable curve
can be generated (voltage vs. airflow velocity).
The thermistors used are glass bead and coated and can be cleaned by gently using a cotton swab and alcohol.
Formaldehyde gas, Hydrogen Peroxide and Chlorine Dioxide has no effect on the airflow sensors; however, the
formaldehyde/Ammonium bicarbonate residue that remains after decontamination should be removed from the
airflow sensor thermistors.
Disconnect electrical power from the cabinet before
! CAUTION
attempting any maintenance action.
The airflow sensors are removed by turning the locking ring counterclockwise and gently pulling the sensor away from
the connector. To reattach the airflow sensor, turn sensor in keyed connector until key matches, push in and turn the
locking ring clockwise until ring locks.
Once the new sensor(s) have been replaced, proceed to the digital sensor setup procedure in section 8.
8.0 Error Messages, Troubleshooting, Option‐Diagnostics & Airflow Sensor Performance Verification
Audible alarms and error messages occur for a variety of reasons. Whenever an alarm condition has been present for a
period of at least 10 seconds, the audible alarm/error message will be presented and stay on until the error is cleared. The
audible alarm will be on for 30 seconds upon initial alarm condition, then once every ten seconds. When presented with an
error message, please perform the following:
Step 1: NOTE ALL ERROR MESSAGES.
Error message will appear in place if the NuAire logo with “Active Alarms” and the alarm type below.
Step 2: VERIFY ERROR MESSAGES.
Error messages can be verified by cleaning the error function by either turning the blower or the cabinet on
and off.
Step 3: MONITOR RE‐OCCURRENCE OF ERROR MESSAGES.
If re‐occurrence of the error message is immediate or daily, use guide below to correct the situation.
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8.1 Error Message Troubleshooting Guide
Error Message Indicator Correction
Window Alarm Sliding window is above its standard working Verify standard working height and window micro switch
(Window High) height or micro switch is not operating properly. operation.
Window Alarm
Sliding window is below its standard working Verify standard working height and window micro switch
(Window Low, only with
height or micro switch is not operating properly. operation.
power window option)
Airflow Alarm
Downflow airflow fell below its lower limit alarm
Red Downflow Arrow Re‐certify cabinet to proper airflow setpoints.
setpoint.
(Downflow Low Limit)
Airflow Alarm
Downflow airflow went above its high alarm
Red Downflow Arrow Re‐certify cabinet to proper airflow setpoints.
setpoint.
(Downflow High Limit)
Airflow Alarm
Inflow airflow fell below its lower limit alarm Check orientation of exhaust sensor shroud.
Red Inflow Arrow
setpoint. Re‐certify cabinet to proper airflow setpoints.
(Inflow Low Limit)
Airflow Alarm
Check orientation of exhaust sensor shroud.
Red Inflow Arrow Inflow Airflow went above its high alarm setpoint.
Re‐certify cabinet to proper airflow setpoints.
(Inflow High Limit)
Low Pressure Alarm
Indicates low pressure or low cabinet airflow Re‐certify cabinet to proper airflow setpoints.
(Low pressure Limit)
Check light fuse on main control board.
Check fluorescent lamps.
Cabinet fluorescent lights
Check voltage coming out of main control bd. to light ballast's.
won't turn on.
Check light starters, if present.
Check ballast.
Check blower fuse on main control board.
Check AC voltage coming out of main control bd.
Cabinet blower
Check wiring to blower.
won't turn on.
Check blower motor.
Check DC motor PWM signal on main control bd.
Indicates that the remote
Display indicates
override is activated,
(Remote Override Active)
preventing the usage of the cabinet
Power Loss Alert! Indicates a power interruption has occurred. Press display to clear message.
Cabinet outlets Check outlet fuse located on main control board.
won't turn on. Check voltage coming out of main control board.
Check sliding window position so that it's fully closed.
Check blower/lights fuse on main control board.
Cabinet ultraviolet light Check voltage coming out of the main control bd. to ultraviolet
won't turn on. light ballast.
Check light starters, if present.
Check ballast.
Blower or light fuse Check for short on output of fuse.
continues to blow Isolate output of fuse by disconnecting control center connectors,
after replacement. light circuit, AC or DC blower circuit, etc. to isolate short.
Replace UV Light! Indicates that the UV light needs replacement Replace UV light and clear UV run time clock.
Display indicates Indicates that the night setback is activated,
(Night Setback Active) preventing the usage of the cabinet.
Try to fully open the window to initiate the self‐calibration
feature. Activate the auto‐reverse function by placing a soft
Power window option is
Window does not open and close correctly. object, i.e. rubber stopper under window and lower until window
malfunctioning.
auto reverses and is raised to its self‐calibrating point.
Re‐calibrate power window memory points.
Check connectors and wires from main control board to the
Active Alarms
Indicates a digital communications error from the airflow sensors.
DN Sensor Comm!
main control board to the airflow sensors. DN indicated downflow sensor.
EX Sensor Comm!
EX indicates exhaust sensor.
Active Alarms
Check airflow probe connector on main board. (Ref. Section 7.10).
DN Sensor Error! Indicates an error signal generated by the sensor.
Replace airflow sensor if required.
EX Sensor Error!
OM0222 Page 73 of 107
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8.2 Calibration/Service Menu
8.2.1 General
As with the airflow calibration process, the service menu should only be accessed by a Service Technician that is
familiar with the product.
MENU
UV
FL
UP
CALIBRATION/SERVICE
TIME/DATE
DOWN
PASSWORD
CABINET DECONTAMINATION
ENTER
SCREEN SETUP
FILTER STATUS
xx:xx AM xx/xx/xx
Entry into the Calibration/Service functions requires a service password for entry. After pressing the
Calibration/Service menu item, a password screen will appear. The default password is “9876”. Once the service
password is entered, the Calibration/Service menu will appear.
As a special feature for the service technician, by accessing the Calibration/Service menu, the service technician
can bypass the blower warm up time itself. This feature remains on for one hour from the time the
Calibration/Service menu was accessed. When bypassing the warm up time, it is not uncommon to experience a
brief alarm as the blower stabilizes at setpoint.
0 5 MENU
EXIT
UV
1 6 FL
BACK
2 7 ENTER PASSWORD
XXXX
ENTER
3 8
4 9 xx:xx xx/xx/xx
AM
8.2.2 Calibration/Service Menu
The Calibration/Service menu provides a list of sub‐menu items to accomplish all service tasks. Each sub‐menu
item will be described in the following sections.
MENU
UV
FL
UP
SETPOINTS/LIMITS
SENSOR SETUP
NIGHT SETBACK
SERVICE
DOWN
OPTION SETUP
DIAGNOSTICS
ENTER
CABINET RESET
CABINET TYPE
xx:xx AM xx/xx/xx
OM0222 Page 74 of 107
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8.2.2.1 Cabinet Type/Motor Type
The cabinet type can be verified or changed in the control system. The cabinet type default information
controls unit of measure, setpoints and limits based on the type and size of cabinet.
To verify, press CABINET TYPE. The current type of cabinet will be designated. Again to verify, press the
correct Cabinet Type and the Model/Size of the current selected cabinet model will be designated. Once
verified the correct type and model are designated, then press MENU to return to Calibration/Service menu.
CURRENTLY A2 NU-480-300/400/500/600 MENU
UV
FL UP
TYPE A2 (70% RECIRCULATE/ 30% EXHAUST)
TYPE B1 (30% RECIRCULATE/ 70%EXHAUST)
DOWN
TYPE B2 (100% EXHAUST)
SET OEM DISPLAYED ENTER
SET MOTOR TYPE
xx:xx AM xx/xx/xx
CURRENTLY A2 NU-480-300/400/500/600
MENU
UP
A2 NU-440-300/400/500/600
A2 NU-440-300/400/500/600(E)
DOWN
A2 NU-440-600-400/600(D)
UV
FL
A2 NU-480-300/400/500/600 ENTER
A2 NU-480-300/400/500/600(E)
A2 NU-480-600-400/600(D)
xx:xx AM xx/xx/xx
Press SET MOTOR TYPE to verify correct setting. Upon a MASTER RESET, the motor type is defaulted to AC.
The NU‐480 Series 1 (115Vac) requires the motor type selected to be DC.
Always verify motor type when verifying cabinet type.
OM0222 Page 75 of 107
March/2014
8.2.2.2 Setpoints/Limits
The airflow setpoints and alarm limits may also be verified or altered. Typically these default values are
factory set based on the cabinet type, model and size as previously discussed. However, they may be altered
in special cases for modified cabinets. The setpoint establishes the airflow values that are to be maintained.
The high low limits establish the alarm boundaries from the nominal setpoint. The default values have been
established based upon the performance specifications and cabinet component tolerances.
To verify or alter any of the airflow setpoints or alarm limits, press the menu setpoints/limits menu item.
Then, press any of the individual setpoints or alarm limits to verify and/or change. Press UP or DOWN to
change new value. Press SAVE to enter new value.
MENU
UV FL
DOWNFLOW SETPOINT UP
DOWNFLOW HIGH LIMIT
DOWNFLOW LOW LIMIT DOWN
EXHAUST FLOW SETPOINT
ENTER
EXHAUST FLOW HIGH LIMIT
EXHAUST FLOW LOW LIMIT
xx:xx AM xx/xx/xx
MENU
UV
FL
UP
OLD DOWNFLOW SETPOINT 60 fpm
NEW DOWNFLOW SETPOINT 60 fpm
DOWN
USE UP/DOWN TO CHANGE NEW DOWNFLOW
SETPOINT, PRESS SAVE TO ENTER NEW
VALUE.
SAVE
PRESS MENU TO EXIT WITHOUT CHANGE
xx:xx AM xx/xx/xx
Default values for NU‐480
Downflow setpoint ‐ 60
Downflow high limit ‐ 66
Downflow low limit ‐ 54
Inflow setpoint ‐ 105
Inflow high limit ‐ 120
Inflow low limit ‐ 90
OM0222 Page 76 of 107
March/2014
8.2.2.3 Digital Sensor Setup
The sensor setup menu is used for both calibration and sensor replacement if necessary. For sensor
calibration process, see airflow calibration section. For sensor replacement, use the “Find new downflow /
exhaust” menu’s below. This would also be required after performing a MASTER RESET.
If finding or replacing one or both sensors, the downflow sensor will always have to be connected and found
first. To find or replace one or both sensors, perform the following procedure:
Disconnect power to cabinet.
NOTE: A power switch is available in the control center to turn the cabinet on and off. Connection and
disconnection must always be performed with the cabinet power off.
Replace one or both airflow sensors.
Reconnect only the downflow sensor at this time.
Leave exhaust sensor connector that runs to the main control board disconnected.
Reconnect power to cabinet and navigate to the sensor setup menu.
Select “FIND NEW DOWNFLOW SENSOR”, and follow menu.
Press” LEARN” to find sensor. If successful, display will indicate SENSOR FOUND, if not, display may
indicate SENSOR ALREADY USED or FAILED TO FIND SENSOR. If this is the case, perform MASTER
RESET and start process over.
MENU
UV
FL
UP
CALIBRATE DOWNFLOW SENSOR
CALIBRATE EXHAUST SENSOR DOWN
FIND NEW DOWNFLOW SENSOR
FIND NEW EXHAUST SENSOR ENTER
xx:xx AM xx/xx/xx
Once downflow sensor is found, turn off the power to cabinet and reconnect exhaust sensor and
disconnect downflow sensor.
Turn power back on and select “FIND NEW EXHAUST SENSOR”.
MENU
UV
FL
CONNECT DOWNFLOW SENSOR TO THE
CABINET AND REMOVE EXHAUST FLOW
LEARN
SENSOR.
PRESS “LEARN” TO FIND SENSOR.
PRESS THE LEARN KEY WHEN READY.
xx:xx AM xx/xx/xx
Press “LEARN” to find sensor. If successful, display will indicate SENSOR FOUND, if not display may
indicate SENSOR ALREADY USED or FAILED TO FIND SENSOR. If this is the case, perform MASTER
RESET and start process over.
Once exhaust sensor is found, turn off power to cabinet, reconnect downflow sensor and turn
cabinet power back on.
Once both downflow and exhaust sensors are found proceed immediately to airflow calibration
sections. (i.e. calibrate downflow sensor)
OM0222 Page 77 of 107
March/2014
8.2.2.4 Night Setback
The optional night setback is used to allow the cabinet to run at a reduced airflow rate to keep the workzone
sterile and maintain containment in a static environment. The night setback is calibrated as a percentage of
the nominal setpoint and should be adjusted during the certification process to the desired level.
Once the night setback is initiated, the airflow alarms and fluorescent light will be inhibited to prevent
cabinet usage. When calibrating the night setback, use the night setback menu below.
MENU
UV
FL
NIGHT SETBACK UP
SETBACK = 80 PERCENT
USE UP/DOWN TO CHANGE PERCENTAGE. DOWN
PRESS SAVE TO SAVE NEW VALUE
WHEN NIGHT SETBACK CONTACTS ARE
CLOSED, THE BLOWER (TYPE A2) OR EXHAUST
DAMPER (TYPE B1/B2) WILL RUN AT THIS SAVE
PERCENTAGE OF ITS NORMAL SETPOINT.
VALID RANGE IS 30% TO 80%.
xx:xx AM xx/xx/xx
8.2.2.5 Service
The service setup menu allows a QUALIFIED TECHNICIAN to configure, calibrate and obtain functional service
data. Each parameter submenu will be described as well as the display will indicate present and/or default
conditions as shown.
MENU
UV
FL
UP
RUN TIMES
MAIN COMPONENT REPLACEMENT
SERIAL NUMBER DOWN
CERTIFICATION DATE
FILTER STATUS DATA
ENTER
PRESS MENU TO EXIT.
xx:xx AM xx/xx/xx
OM0222 Page 78 of 107
March/2014
Run Times
This parameter allows the service technician to view, alter, or reset both UV light and motor blower run timer.
Select desired run time parameter from menu.
MENU
UV
FL
UP
UV RUN TIME
DOWN
BLOWER MOTOR RUN TIME
ENTER
xx:xx AM xx/xx/xx
UV run time.
MENU
UV
FL
UV LIGHT USAGE
CURRENT HOURS = XXXX
RESET
THIS TIME SHOWS HOW MANY HOURS THE UV
LIGHT HAS LOGGED ON. RESET THE TIME WHEN
REPLACING THE UV BULB.
PRESS MENU TO EXIT WITHOUT RESET
xx:xx AM xx/xx/xx
Motor blower run time.
MENU
UV
FL
UP
MOTOR BLOWER RUN TIME
xx:xx AM xx/xx/xx
OM0222 Page 79 of 107
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Main Component Replacement
This parameter allows the service technician to view and update HEPA filters, blower motor, main control board
and sensor installation dates.
MENU
UV
FL
SUPPLY HEPA FILTER MM / DD / YYYY UP
EXHAUST HEPA FILTER MM / DD / YYYY
BLOWER MOTOR MM / DD / YYYY
MAIN CONTROL BOARD MM / DD / YYYY DOWN
DOWNFLOW SENSOR MM / DD / YYYY
EXHAUST SENSOR MM / DD / YYYY
PRESSING SAVE WILL SET THE ITEM POINTED SAVE
TO BY THE CURSOR TO TODAYS DATE.
PRESS MENU TO EXIT.
xx:xx AM xx/xx/xx
MENU
UV
FL
SETTING___________ _________ REPLACEMENT DATE
UP
< MM / DD / YYYY
DOWN
>
SAVE
USE >> AND << TO SELECT MONTH, DAY OR YEAR.
USE UP/DOWN TO CHANGE VALUE.
PRESS SAVE TO ENTER NEW SETTING. xx:xx AM xx/xx/xx
Serial Number
This parameter allows the service technician to view and enter the cabinets’ serial number
MENU
UV
FL
SERIAL NUMBER
XXXXXXXXXXXX CHANGE
PRESS CHANGE TO ENTER SERIAL NUMBER
PRESS MENU TO EXIT
xx:xx AM xx/xx/xx
Once CHANGE is pressed, a display screen very similar to the password display screen will appear for entry of
the serial number. Press ENTER to save the serial number.
OM0222 Page 80 of 107
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Certification Date
This parameter allows the service technician to view and update the current certification date.
MENU
UV
FL
LAST CERTIFICATION DATE UP
MM / DD / YYYY
DOWN
PRESSING SAVE WILL SET THE CERTIFICATION DATE
TO TODAYS DATE. (MM / DD / YYYY)
SAVE
PRESS DUE TO SET WHEN NEXT CERTIFICATION
WILL BE REQUIRED.
xx:xx AM xx/xx/xx
MENU
UV
FL
CERTIFICATION REQUIRED TIME PERIOD
CURRENT PERIOD = NO REMINDER UP
DOWN
PRESS UP/DOWN TO CHANGE TIME PERIOD.
PRESS SAVE TO ENTER NEW TIME PERIOD.
THIS SETS THE TIME IN WHICH A NEW CERTIFICATION IS
REQUIRED. A REMINDER WILL APPEAR STARTING SAVE
4 WEEKS BEFORE THIS TIME EXPIRES FROM THE LAST
CERTIFICATION.
xx:xx AM xx/xx/xx
Filter Status Data
This parameter allows the service technician to set the filter status data used to predict filter life availability.
Filter status is based on maximum RPM minus the starting RPM (entered by technician) then scaled to current
RPM to determine filter percentage availability. Starting RPM data may be entered at any time for service
purposes.
MENU
DC MOTOR FILTER STATUS DATA
OM0222 Page 81 of 107
March/2014
Power Window
This parameter allows for the power window option to operate. When the power window option is factory
installed this parameter will allow the user display menu to indicate the power window up and down icons.
The power window option functions using a 24 Vdc motor and internal feedback encoder to determine window
position. The power window also incorporates a safety auto reverse feature to eliminate any pinch hazard.
Select desired power window parameter from menu.
MENU
UV
FL
UP
ENABLE POWER WINDOW
POWER WINDOW TEST
WINDOW STEP
DOWN
ENTER
PRESS MENU TO EXIT
xx:xx AM xx/xx/xx
Enable Power Window
Select power window option on or off.
MENU
UV
FL
POWER WINDOW
OPTION IS ON ON
OFF
xx:xx AM xx/xx/xx
OM0222 Page 82 of 107
March/2014
Power Window Test
Power window test allows the movement of the power window for all positions including the lowest position,
with the armrest removed, used for the decon process.
MENU
UV
FL
AUTO WINDOW TEST
PRESS THE WINDOW KEYS TO MOVE
THE WINDOW THROUGH ITS WORKING
RANGE
xx:xx AM xx/xx/xx
Window Step
MENU
UV
FL
WINDOW STEP
PRESS THE WINDOW KEYS TO MOVE THE WINDOW.
WINDOW WILL STOP WHEN KEY IS RELEASED AND
NOT CONTINUE TO THE AUTO STOP POSITIONS.
xx:xx AM xx/xx/xx
Power Window Calibration
The power window is calibrated by the micro switch position within the left window glide assembly. To access the
micro switches for adjustment, remove the left faring rear access cover via fasteners. Micro switches are then
adjustable using a Phillips screwdriver.
Continues to travel down for 1/8” (3mm)
Top Middle Window High Alarm
past switch deactivation
Continues to travel up for 1‐7/8” (48mm)
Bottom Middle Window Low Alarm past switch deactivation
(Used only with power window option)
OM0222 Page 83 of 107
March/2014
8.2.2.6 Option Set Up
The option set up menu allows A QUALIFIED TECHNICIAN to configure several different optional parameters
per the menu below. Each parameter sub‐menu will be described as well as the display will the default
conditions as shown.
MENU
UV
FL
AIRFLOW DISPLAY AVERAGING
UP
UV WINDOW INTERLOCK
CONTROL/ALARM MANUAL CONTROL
ALARM SILENCE
DOWN
EXHAUST UPDATE
MOTOR BLOWER LOCKOUT
BLOWER WINDOW INTERLOCK
ENTER
AUX RELAY FUNCTION
PRINTER REPORT
MORE OPTIONS
xx:xx AM xx/xx/xx
MENU
UV
FL
UP
BLOWER FL LIGHT INTERLOCK
EXHAUST AUTO CAL
ACCESSORY OUTLET DOWN
NIGHT SETBACK WINDOW INTERLOCK
FAN RELAY INTERLOCK
ALARM SILENCE TIME SAVE
DOWNFLOW UPDATE TIME
DOWNFLOW ALARM TIME
MORE OPTIONS xx:xx AM xx/xx/xx
Airflow Display Averaging
This parameter allows for the selection of the airflow display averaging function to operate. When the airflow
averaging is on, the downflow display will always indicate the airflow setpoint 60fpm (0.30m/s) if the airflow is
valid and within its alarm limits. The exhaust display will use the exhaust update averaging function.
MENU
UV
FL
ON
AIRFLOW AVERAGING OPTION IN ON
OFF
WHEN ON, THE DOWNFLOW DISPLAY
SHOWS SETPOINT AND EXHAUST
DISPLAY SHOWS AVERAGE DATA AS SET SAVE
BY EXHAUST UPDATE OPTION.
xx:xx AM xx/xx/xx
OM0222 Page 84 of 107
March/2014
UV Window Interlock
This parameter allows for the selection of the window closed switch to be interlocked with the UV light option.
When the window interlock is on, the window must be closed for the UV light to operate. When the window
interlock is off the UV light can be turned on regardless of the window position.
NOTE: In addition to the TOUCHLINK system UV window interlock there is a double redundant UV window
interlock relay. To override the UV window interlock for service purposes only, both interlocks must be
changed through the TOUCHLINK system and shorting the relay connection.
(See electrical schematic for reference)
MENU
UV
FL
WINDOW INTERLOCK ON
OPTION IS ON
OFF
WHEN THE WINDOW INTERLOCK IS ON, THE
WINDOW MUST BE CLOSED FOR THE UV SAVE
LIGHT TO OPERATE. WHEN OFF, THE
WINDOW CAN BE IN ANY POSITION.
xx:xx AM xx/xx/xx
Control/Alarm Manual Control
This parameter allows ONLY THE CABINET TECHNICIAN to run the cabinet in manual mode. This means with no
controls or alarms activated. When the manual control is on, the downflow and inflow displays will indicate
nominal setpoints. Airflow adjustments can be made in the manual mode by going into airflow calibration and
adjusting the blower duty cycle. The blower duty cycle will remain constant in manual mode. The display will also
indicate the manual control is activated. When the manual control is off, full automatic control resumes.
MENU
UV
FL
MAIN CONTROL OPTION IS OFF ON
WHEN MANUAL CONTROL IS ON, BLOWER OFF
SPEED CAN BE SET MANUALLY AND
AIRFLOW ALARMS ARE DISABLED. WHEN
OFF, THE BLOWER SPEED IS CONTROLLED
AUTOMATICALLY, AND AIRFLOW ALARMS SAVE
FUNCTION NORMALLY. xx:xx AM xx/xx/xx
OM0222 Page 85 of 107
March/2014
Alarm Silence
This parameter allows for the selection of the alarm silence key function. When the alarm silence function is on,
all current and future alarms will be silenced for the designated alarm silence time (i.e. default time is 15
minutes). When the alarm silence function is off, all current alarms will be silenced for the designated alarm
silence time.
If a new alarm is present, the audible alarm will again be turned on.
MENU
UV
FL
ALARM SILENCE OPTION IS ON ON
WHEN ALARM SILENCE IS ON, PRESSING SILENCE
ICON DISABLES ALL EXISTING AND NOW AUDIBLE OFF
ALARMS FOR THE DESIGNATED ALARM SILENCE
TIME. WHEN OFF, ONLY WINDOW HIGH ALARMS
MAY BE SILENCED WITH THE ICON FOR THE ALARM
SILENCE TIME SAVE
xx:xx AM xx/xx/xx
Exhaust Update
This parameter allows for the selection of time to determine how much exhaust flow data is averaged before
being displayed. The time is displayed in seconds with a programmable range from 1 to 60
MENU
UV
FL
EXHAUST UPDATE
UP
OPTION = 30
DOWN
THIS OPTION DETERMINES HOW MANY
SECONDS OF EXHAUST DATA IS AVERAGED
BEFORE BEING DISPLAYED. VALID RANGES
ARE 1 TO 60 SECONDS.
SAVE
xx:xx AM xx/xx/xx
OM0222 Page 86 of 107
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Motor Blower Lockout
This parameter allows the access to turn the blower on or off to be restricted by the use of a password. When the
blower lockout is on, pressing the blower icon will produce a numerical password screen. The default password is
“1234” and may be changed using the password menu. When the blower lockout is off, the blower may be turned
on and off without restriction.
MENU
UV
FL
ON
BLOWER LOCKOUT OPTION IS ON
OFF
WHEN BLOWER LOCKOUT IS ON, A PASSWORD IS
REQUIRED TO TURN THE BLOWER ON OR OFF.
WHEN BLOWER LOCKOUT IS OFF, NO PASSWORD
IS REQUIRED.
SAVE
xx:xx AM xx/xx/xx
Blower Window Interlock
This parameter allows for the selection of the window closed switch to be interlocked to the blower. When the
blower interlock is on, the blower will turn off when the window is closed. When the blower is off, the blower will
continue to run when the window is closed.
MENU
UV
FL
ON
BLOWER WINDOW INTERLOCK
OPTION IS ON
OFF
WHEN BLOWER WINDOW INTERLOCK IS ON, THE
BLOWER WILL TURN OFF WHEN WINDOW IS
CLOSED. WHEN OFF, THE BLOWER WILL
SAVE
CONTINUE TO RUN.
xx:xx AM xx/xx/xx
OM0222 Page 87 of 107
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AUX Relay Function
This parameter allows for the selection of the AUX relay function. When the AUX relay is on, the AUX relay
function will be identical to the fan relay. When the AUX relay function is off, the AUX relay function provides
delay On/Off option.
MENU
UV
FL
ON
AUX RELAY FUNCTION OPTION IS ON
OFF
WHEN AUX RELAY FUNCTION IS ON, THE RELAY
FUNCTION WILL BE IDENTCAL TO THE FAN RELAY.
WHEN OFF, THE RELAY CAN BE USED FOR SAVE
EXHAUST DELAY ON/OFF OPTIONS.
xx:xx AM xx/xx/xx
Printer Report Frequency
This parameter allows for the selection of the frequency of the RS‐232 Communication Output. The
Communication Output provides communication from the cabinet to a personal computer (HyperTerminal) or
printer via RS‐232 interconnect standard. RS‐232 provides short range (50 feet) simple point‐to‐point connection
with another RS‐232 device.
The Communication Interface utilizes 9‐bit character frame with eight bits (no parity) and a stop bit (8.N.1) with a
constant transmission speed of 57,600 bps for communication.
The connection for the RS‐232 output is located on the main control board as a RJ‐45 (8‐pin) connector (J5).
The following is the pin position for the connector:
1 ‐ NOT USED 5 ‐ NOT USED
2 ‐ NOT USED 6 ‐ NOT USED
3 ‐ GROUND 7 ‐ TRASMIT
4 ‐ NOT USED 8 ‐ RECEIVE
The Communication Output provides airflow readings as shown below.
Actual airflows with setpoints (in parentheses) are output.
MENU
UV
FL
ON
PRINTER REPORT FREQUENCY
OPTION SETTING = 0
OFF
THIS OPTION DETERMINES HOW OFTEN THE
CONTROLLER OUTPUTS THE CURRENT STATUS
SAVE
TO A PRINTER. VALID CHANGES ARE 1 TO 60
MINUTES. SETTING THE FREQUENCY DISABLES
THE OUTPUT.
xx:xx AM xx/xx/xx
OM0222 Page 88 of 107
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Blower FL Light Interlock
This parameter allows for the selection of the fluorescent light option to be interlocked to the blower. When the
blower FL light interlock is on, the fluorescent light operation will be interlocked to the blower. When the blower
FL light interlock is off, the fluorescent light can be turned on at any time.
MENU
UV
FL
BLOWER FLOURSCENT LIGHT INTERLOCK ON
OPTION IS OFF
OFF
WHEN BLOWER FL LIGHT INTERLOCK IS ON THE FL
LIGHT WILL TURN ON WHEN THE BLOWER IS
TURNED ON AND CAN ONLY BE TURNED ON WHEN SAVE
THE BLOWER IS ON. WHEN OFF, THE FL LIGHT CAN
BE TURNED ON AT ANYTIME.
xx:xx AM xx/xx/xx
Exhaust Auto CAL
This parameter allows the Exhaust Auto CAL function to operate. The Exhaust Auto CAL function automatically
performs a calibration offset to the setpoint value for the inflow display. The calibration offset is performed at the
end of the warm‐up cycle, then 1 hour after the warm‐up cycle and every 4 hours after that. The Exhaust Auto
CAL function is based upon the fact that the cabinet has a common plenum between the supply and exhaust
(inflow) airflow. The blower air volume is split between the supply (70%) and the exhaust (30%) airflow. Typically
these values being in a common plenum will rise and fall together so if the blower duty cycle or voltage is constant
or within tolerance, the cabinet airflows will also be within the acceptable tolerance range and the auto CAL
function will occur. If the blower duty cycle or voltage is not within the specified tolerance, the auto CAL function
will not occur.
MENU
UV
FL
OFF
xx:xx AM xx/xx/xx
OM0222 Page 89 of 107
March/2014
Accessory Outlet
This parameter allows for the selection of the power connection that supplies power to the exhaust decon
chamber to be interlocked to the blower. This parameter would only be used in special cases and does not affect
operation during an auto decon cycle.
MENU
UV
FL
ON
ACCESSORY OUTLET OPTION IS OFF
OFF
WHEN ACCESSORY OUTLET IS ON, THE
EXTERNAL OUTLET WILL TURN ON/OFF AS THE
SAVE
BLOWER TURNS ON/OFF
xx:xx AM xx/xx/xx
Night Setback Window Interlock
This parameter allows for the selection of the night setback function to be initiated upon a sliding window closure.
Once the sliding window is closed, the fluorescent light will be disabled and the airflow will be reduced to a
percentage of the normal setpoint.
NOTE: Blower window interlock must be turned off to allow the night setback function to operate.
MENU
UV
FL
ON
NIGHT SETBACK WINDOW INTERLOCK OPTION IS OFF
WHEN NIGHT SETBACK WINDOW INTERLOCK IS ON, OFF
CLOSING THE WINDOW WILL INITIATE A NIGHT
SETBACK AND THE BLOWER WILL RUN A LOWER
PERCENTAGE OF THE NORMAL SETPOINT. SAVE
FLUORESCENT LIGHT WILL ALSO TURN OFF.
xx:xx AM xx/xx/xx
Fan Relay Interlock
This parameter allows for the selection of the fan relay interlock operation. When the fans relay interlock is on,
and the blower switch is pending or blue, the fan relay will be off or not energized. If the fan relay interlock is off
and the blower switch is pending or blue, the fan relay will be on or energized. In either case the fan relay will be
on when the blower switch is on or green and off when the blower switch is off or not colored.
MENU
UV
FL
ON
FAN RELAY INTERLOCK IS OFF
WHEN THE FAN RELAY INTERLOCK IS ON AND THE OFF
BLOWER SWITCH IS PENDING OR BLUE, THE FAN
RELAY WILL BE OFF. IF THE FAN INTERLOCK RELAY
IS OFF AND THE BLOWER SWITCH IS PENDING OR ENTER
BLUE, THE FAN RELAY WILL BE ON.
xx:xx AM xx/xx/xx
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Alarm Silence Time
This parameter allows for the selection of time to determine how long the audible alarm shall be silenced.
The time is displayed in minutes with a programmable range of 1 to 60.
MENU
UV
FL
ON
ALARM SILENCE TIME
OPTION SETTING = 15
OFF
THIS OPTION DETERMINES HOW MANY MINUTES
THE AUDIBLE ALARM WILL BE SILENCED WHEN
SILENCE IS PRESSED. ENTER
VALID RANGES ARE 1 - 60 MINUTES
xx:xx AM xx/xx/xx
Downflow Update Time
This parameter allows for the selection of time to determine how much downflow data is averaged before being
displayed. The time is displayed in seconds with a programmable range from 4 to 12 seconds.
MENU
UV
UV FL
FL UP
DOWNFLOW UPDATE ON
OPTION SETTING TIME
ALARM SILENCE =8
OPTION SETTING = 15 DOWN
THIS OPTION DETERMINES HOW MANY SECONDS OFF
OF DOWNFLOW DATA IS AVERAGED BEFORE
THIS OPTION DETERMINES HOW MANY MINUTES
BEING DISPLAYED.
THE AUDIBLE ALARM WILL BE SILENCED WHEN SAVE
VALID RANGES
SILENCE ARE 4 TO 12 SECONDS.
IS PRESSED. ENTER
VALID RANGES ARE 1 - 60 MINUTES xx:xx AM xx/xx/xx
Downflow Alarm Time
This parameter allows for the selection of time to determine how many continuous seconds of an alarm condition
occurs before activating an audible and visual alarm. The time is displayed in seconds with a programmable range
from 2 to 12 seconds.
MENU
xx:xx AM xx/xx/xx
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8.2.2.7 Diagnostics
The diagnostics menu allows A QUALIFIED TECHNICIAN to exercise the control system’s inputs and outputs.
Each of these has its own menu screen to excise the control system. Select menu screen as desired.
MENU
UV
FL
UP
DOWN
TEST OUTPUTS
READ INPUTS
ENTER
xx:xx AM xx/xx/xx
Test Outputs
Test outputs allow a service technician to exercise these output functions. Pressing TOGGLE will turn on and
off the functions. Press UP and DOWN to select the test output desired. Use the display icons to turn on/off
each function of blower, UV light, fluorescent light, outlet and optional power window if installed.
MENU
UV
FL
UP
ACCESSORY OUTLET OFF
DAMPER OFF
GAS SOLENOID OFF DOWN
ALARM LED OFF
AUX RELAY OFF
ALARM RELAY OFF TOGGLE
FAN RELAY OFF
AUDIBLE ALARM OFF
xx:xx AM xx/xx/xx
CYCLE ALL OFF
Read Inputs
Read inputs all a service technician to exercise or check these input functions. The inputs may be checked by
altering the state of the input function (i.e. sliding window position) and monitoring the change on the display.
MENU
UV
FL
KEYPAD X = 0, Y = 0
DOWNFLOW SENSOR 60 FPM
EXHAUST SENSOR 105 FPM
REMOTE OVERRIDE OPEN
NIGHT SETBACK OPEN
WINDOW POSITION NORMAL
PRESSURE SENSOR OPEN NEXT
AC LINE HZ 60 HZ
AC LINE VOLTS 115 VAC
MOTOR RPM 1000 RPM
xx:xx AM xx/xx/xx
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MENU
UV
FL
WINDOW HIGH LIMIT CLEAR
WINDOW HIGH CLEAR NEXT
WINDOW LOW CLEAR
WINDOW CLOSED CLEAR
WINDOW SAFE TRIPPED
xx:xx AM xx/xx/xx
8.3 Airflow Sensor Performance Verification
The individual airflow sensors can be routinely checked during calibration or in diagnostics to assure they are reading and
active within the range of use (0 to 200 fpm). The airflow sensors can also be checked in the run mode through
performance verification, for responsiveness to changing airflow conditions.
8.3.1 Run Mode
To check the airflow sensor in run mode, first allow the cabinet to operate normally for a minimum of 5 minutes.
Then, place a rolled piece of paper over the downflow sensor in the workzone and leave the paper on the sensor
for at least 2 minutes and then remove. This action will cause the cabinet to go into a downflow alarm condition.
The exhaust airflow reading should increase during this test. However, the downflow reading should go down to
zero on the display “0”. There should also be a noticeable increase in motor/blower noise. It would also be
recommended to monitor motor/blower voltage during the test. The motor/blower voltage should be monitored
from when the cabinet is running normally. During the test, when the downflow sensor is covered, the
motor/blower voltage should be steadily increasing too slightly under line voltage. When the downflow sensor is
uncovered, the motor/blower voltage should decrease and airflow readings should be within the calibration range.
If the motor/blower voltage does not change, an airflow sensor problem could exist. Please consult with NuAire
Technical Service.
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9.0 Remote Contacts
The NU‐480 has several contact closures for remote sensing of various functions.
9.1 Fan Relay
The fan relay contacts are dual normally open contact closure outputs which are activated whenever the blower is turned
on. Contact ratings are 250 VAC maximum at 2 Amps.
9.2 Alarm Relay
The alarm relay contacts are dual normally open contact closure outputs which are activated whenever an airflow alarm
condition occurs. An airflow alarm condition will occur if either airflow sensor detects 5 consecutive 2 second airflow
readings above or below the alarm limits. Contact ratings are 250 VAC maximum at 2 Amps.
9.3 AUX Relay
The AUX relay contacts are common, normally open and normally closed contact closure outputs which are activated
whenever the bower is turned on. However, the AUX relay does have some conditional logic programmed. The relay will
activate whenever the blower is turned on and stay on unless after 5 minutes there is a low exhaust alarm, then the relay
will deactivate. If exhaust is sufficient, the relay will stay active. If the blower is then turned off, the relay will stay active
for one minute then deactivate. The AUX relay may also be selected to operate the same as the fan relay. Reference the
AUX relay function in the option menu. Contact ratings are 250 VAC maximum at 2 Amps.
9.4 Remote Override
The remote override contacts are (no power) shorting contacts only, which when closed, indicates to the control system to
shut down the cabinet. The blower would be turned off, an audible alarm would be turned on for several seconds and the
clock display will indicate "RM.OV".
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10.0 Optional Equipment
10.1 Ultraviolet Light
Ultraviolet light will injure your eyes. Avoid direct viewing at all times.
! CAUTION
Personnel should not be present when ultraviolet lamp is on
10.1.1 Overview
The germicidal ultraviolet is primarily intended for the destruction of bacteria and other microorganisms in the air
or on directly exposed surfaces. Approximately 95% of the ultraviolet radiations from germicidal tubes are in the
253.7 nanometer region. This is a region in the ultraviolet spectrum which is near the peak of germicidal
effectiveness. The exposure necessary to kill bacteria is the product of time and intensity. High intensities for a
short period of time, or low intensities for a longer period are fundamentally equal in lethal dosage on bacteria
(disregarding the life cycle of bacteria). The intensity of light falling on a given area is governed by the inverse law;
that is the killing intensity decreases as the distance increases from the tube.
The germicidal tube is placed in the cabinet to provide an average intensity of 100 microwatts per centimeter
(for a new tube) falling on a horizontal plane defined by the bottom of the work surface. The minimum
requirement per paragraph 5.12 of NSF Standard 49 is 40 microwatts per square centimeter (ref. NSF Std. #49,
June, 1976).
Since ultraviolet rays will not penetrate ordinary glass, it is recommended that the sliding window be closed while
the ultraviolet light is on within the cabinet; or that personnel leave the cabinet face area.
10.1.2 Operation
The operation of the ultraviolet light is accomplished by closing the sliding window and pressing the UV switch
located on the front panel. The sliding window is interlocked to the ultraviolet light so, when the sliding window is
raised, the ultraviolet light will turn off. If operational time duration is known, the timer can be used in
conjunction with the ultraviolet light to time out the ultraviolet light operation. This can be accomplished by first
turning on the ultraviolet light. Then, set the timer to the desired length of ultraviolet light operation time. Upon
timer expiration, the ultraviolet light will turn off.
10.1.3 Precaution
The rays from germicidal tubes may cause a painful but temporary irritation of the eyes and reddening of the skin,
if of sufficiently high intensity, or if exposure covers a prolonged period of time. For this reason, one should avoid
direct eye and skin exposure to ultraviolet light. If exposure cannot be avoided, it is necessary for personnel to
wear eye goggles or face shields, and long sleeve gowns with rubber gloves.
Since ultraviolet rays will not penetrate ordinary glass, it is recommended that the sliding window be closed while
the ultraviolet light is on within the cabinet; or that personnel leave the cabinet face area.
10.1.4 Maintenance
The output of an ultraviolet light deteriorates with burning age. The useful life of the light is approximately 7000
hours under specific test conditions.
NOTE: Before testing with lamp off, the light may be cleaned with a lint‐free cloth dampened with alcohol or
ammonia and water.
It is recommended that either a time schedule be established or the tube's output be measured periodically and
the tube replaced when its output falls below 40 microwatts per square centimeter or exceeds 7000 hours of
operation. Lights should be allowed to operate approximately 5 to 10 minutes (longer when the light is in low
temperatures) to warm up sufficiently before reading the output with a meter.
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Energies Required to Destroy Some Microorganisms by Ultraviolet Radiation’s (e)
Microwatt Microwatt
Mold Spores seconds Protozoa seconds
per cm/2 per cm/2
Penicillium roqueforti 26,400 Paramecium 200,000(a)
Penicillium expansum 22,000
Penicillium digitiatum 88,000 Nematode Eggs 40,000(b)
Aspergillus glaucus 88,000
Aspergillus flavus 99,000 Algae 22,000(c)
Aspergillus niger 330,000
Rhizopus nigricans 220,000 Virus
Mucor racemosus A 35,200 Baceriophage (E. Coli) 6,600
Mucor racemosus B 35,200 Tobacco Masaic 440,000
Oospora lactis 11,000 Influenze 3,400(d)
Yeasts
Saccharomyces 13,200
ellipsoideus 17,600
Saccharomyces cerevisiae 13,200
Brewers' yeast 6,600
Baker's yeast 8,800
Common yeast cake 13,200
Bacteria
Streptococcus lactis 8,800
Strep. hermolyticus (alpha type) 5,500
Staphylococcus aureus 6,600
Staphylococcus albus 5,720
Micrococcus sphaeroides 15,400
Sarcina lutea 26,400
Pseudomonas fluorescens 7,040
Escherichia coli 7,040
Proteus vulgaris 7,480
Serratia marcescens 6,160
Bacillus subtilis 11,000
Bacillus subtilis spores 22,000
Spirillum rubrum 6,160
References:
(a) Luckiesh, Matthew (1946) Application of Germicidal, Ethyemal and Infrared Energy,
D. Van Nostrand o., New York, New York, pp 253
(b) Hollaender (1942) Aerobiology, A.A.A.S. (for 90% inactivation), pp 162
(c) Ellis, C. and Wells, O.O. (1941) The Chemical Action of Ultraviolet Rays, Reinhold Publishing Corp.,
pp. 713‐714
(d) Hollaender, A., Oliphant, J.W. (1944) The inactivation effect of monochromatic ultraviolet. Radiation
on Influenze Virus (for 90% inactivation) Jour. of Bact. 48, pp. 447‐454
(e) This table, "Energies Required to Destroy Some Microorganisms by Ultraviolet Radiation's" comes from Westinghouse brochure entitled ‐ "Westinghouse
Sterilamp Germicidal Ultraviolet Tubes"
OM0222 Page 96 of 107
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11.0 Electrical/Environmental Requirements
11.1 Electrical (Supply Voltage Fluctuations Not to Exceed +/‐ 10%)
*NU‐480‐300 115 VAC, 60 Hz, 1 Phase, 12 Amps
*NU‐480‐400 115 VAC, 60 Hz, 1 Phase, 12 Amps
*NU‐480‐500 115 VAC, 60 Hz, 1 Phase, 14 Amps
*NU‐480‐600 115 VAC, 60 Hz, 1 Phase, 14 Amps
*UL/UL‐C Listed
11.2 Operational Performance (for indoor use only)
Environment Temperature Range: 60F‐85F (15C ‐ 30C)
Environment Humidity: 20% ‐ 60% Relative Humidity
Environment Altitude: 6562 Feet (2000M)
11.3 Light Exposure
Standard Fluorescent Lighting @ 150 ft. candles (1614 LUX) maximum intensity.
11.4 Installation Category: 2.0
Installation category (overvoltage category) defines the level of transient overvoltage which the instrument is designed
to withstand safely. It depends on the nature of the electricity supply and its overvoltage protection means. For
example, in CAT II, which is the category used for instruments in installations supplied from a supply comparable to public
mains such as hospital and research laboratories and most industrial laboratories, the expected transient overvoltage is
2500 V for a 230 V supply and 1500 V for a 120 V supply.
11.5 Pollution Degree: 2.0
Pollution degree describes the amount of conductive pollution present in the operating environment. Pollution degree 2
assumes that normally only non‐conductive pollution such as dust occurs with the exception of occasional conductivity
caused by condensation.
11.6 Chemical Exposure
Chemical exposure should be limited to antibacterial materials used for cleaning and disinfecting. USE OF CHLORINATED
OR HALOGEN MATERIALS IN THE CABINET MAY DAMAGE STAINLESS STEEL. Chamber decontamination can be
accomplished by paraformaldehyde, vapor phased Hydrogen Peroxide or Ethylene Oxide without degradation of cabinet
materials.
11.7 EMC Performance (classified for light industrial)
Emissions: EN61326
Immunity: EN61326
Class A equipment is intended for use in an industrial environment. In the
documentation for the user, a statement shall be included drawing attention to
the fact that there may be potential difficulties in ensuring electromagnetic
! WARNING compatibility in other environments, due to conducted as well as radiated
disturbances.
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12.0 Disposal and Recycle
Cabinets that are no longer in use and are ready for disposal contain reusable materials. ALL components with the
exception of the HEPA filters may be disposed and/or recycled after they are known to be properly disinfected.
Note: Follow all local, state and federal guidelines for disposal of HEPA filter solid waste.
BIOHAZARD
Prior to any disassembly for disposal, the cabinet must be decontaminated.
! CAUTION
RECYCLE LEAD FREE
Component Material
Base Cabinet Stainless Steel
Front Grill Stainless Steel
Worksurface Stainless Steel
Window Faring Stainless Steel
Window Glides HDPE
Window Safety Glass
Window Frame Stainless Steel
Front Service Panel Painted Steel
Front Decorative Panel Painted Steel
Control Center Painted Steel
Supply Diffuser Aluminum
Exhaust Filter Aluminum
HEPA Filter Frames Painted Steel
Hepex Bag PVC
Blower Wheel & Housing Steel
Motor Various Steel
Printed Wiring Assembly Lead Free Electronic
Wire PVC Coated Copper
Ballasts Various Steel, Electronic
Armrest PVC
Connectors Nylon
Hardware Stainless Steel and Steel
Note: Material type can be verified with use of a magnet with stainless and aluminum being non‐magnetic.
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