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Calculate The Colony Forming Units

This document provides instructions for calculating colony forming units (cfu) per milliliter of starter culture using proportions. Students are given examples of setting up and solving proportions using the number of colonies counted on plates and their respective dilutions to find the cfu/mL of the culture. Students are then asked to calculate the cfu/mL for samples with given colony counts and dilutions, showing their work.

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Shubhangini Balpande
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482 views2 pages

Calculate The Colony Forming Units

This document provides instructions for calculating colony forming units (cfu) per milliliter of starter culture using proportions. Students are given examples of setting up and solving proportions using the number of colonies counted on plates and their respective dilutions to find the cfu/mL of the culture. Students are then asked to calculate the cfu/mL for samples with given colony counts and dilutions, showing their work.

Uploaded by

Shubhangini Balpande
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOC, PDF, TXT or read online on Scribd
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Science Research 7

Calculate the colony forming units (cfu) per ml. of starter culture. Choose a plate with between 50 and 150 colonies. Calculate the cfu/mL of culture by using the following proportion. # of colonies x dilution of plate X ------------------------------------------------ = -------------volume of culture on plate 1 ml. For example, if you counted 24 colonies on the 105 dilution plate you would use the following proportion and solve for X using the following steps. 1) set up proportion 24 x 105 ------------0.25 X = ------1

2) cross multiply

0.25 x = 1 (24 x 105 )

3) divide to get x alone

0.25 x 1 (24 x 105 ) --------- = ----------0.25 0.25

4) use calculator to solve

(24 x 105 ) ------------0.25

5) put answer in scientific notation X = 9600000 X = 9.6 x 106

Science Research 7

Calculate the cfu/mL of culture. Show all your work!

number of colonies __42__ dilution of plate __106___

number of colonies __75___ dilution of plate ___104___

________________ cfu / mL

________________ cfu / mL

number of colonies __57__ dilution of plate __105___

number of colonies __62___ dilution of plate ___106___

________________ cfu / mL

________________ cfu / mL

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