100% found this document useful (1 vote)

3K views2 pages

Biochemical Test Chart

This document contains a biochemical test chart for identifying several common bacterial species based on their reactions to various biochemical tests. The chart lists the bacterial species down the left column and the biochemical tests across the top row. The results for each test for each species is indicated with "+" for a positive result or "-" for a negative result. Some tests also provide descriptions of the expected results, such as color changes. The chart will aid in identifying unknown bacterial species by comparing their biochemical test results to those listed.

Uploaded by

Shaezarah Mohamudally

We take content rights seriously. If you suspect this is your content, claim it here.

Available Formats

Download as PDF, TXT or read online on Scribd

100% found this document useful (1 vote)

3K views2 pages

Biochemical Test Chart

Uploaded by

Shaezarah Mohamudally

We take content rights seriously. If you suspect this is your content, claim it here.

Available Formats

Download as PDF, TXT or read online on Scribd

You are on page 1/ 2

Sucrose Fermentation

Casease
Gelatin Hydrolysis
Lipase (Tributyrin)
Starch Hydrolysis
Indole
Methyl Red
Voges Proskauer
Citrate

Streptococcus pyogenes

Lactose Fermentation

Streptococcus agalactiae

+ = yellow (Check for gas)

- = red
+ = yellow (Check for gas)
- = red
+ = yellow (Check for gas)
- = red
+ = clearing in agar
- = no clearing in agar
+ = liquid
- = solid
+ = clearing in agar
- = no clearing in agar
+ = no color change in iodine
- = iodine turns blue/black
+ = red lake
- = no color change
+ = red
- = yellow to orange
+ = intense rose/red color
- = yellow
+ = blue
- = green

Staphylococcus epidermidis

Glucose Fermentation

Staphylococcus aureus

+ = endospores (green)
- = no endospores (red)

Serratia marcescens

Endospore Stain

Salmonella typhimurium

+ = capsules (halo around cells)

- = no capsules

Proteus vulgaris

Capsule Stain

Proteus mirabilis

Acid-Fast Stain

Micrococcus roseus

Gram Stain

Micrococcus luteus

+ = motile
- = nonmotile
+ = purple to blue stain
- = red to pink stain
+ = acid fast (red)
- = non-acid fast (blue)

Klebsiella pneumoniae

Motility

Escherichia coli

rods, cocci, pairs, chains,

tetrads, clumps, size, etc.

Enterococcus faecalis

Cellular Morphology

Enterobacter cloacae

surface, consistency, soluble

pigment, optical, size, shape,
margin, elevation, color, etc.

Enterobacter aerogenes

(use standard abbreviations or

devise your own legend)

Bacillus subtilis

Colonial Morphology

Bacillus megaterium

(You will not fill in every blank in this chart.)

Alcaligenes faecalis

Microbiology for the

Health Sciences
Biochemical Test Chart

Pseudomonas aeruginosa

BIOL 2420

Streptococcus pyogenes

MacConkey Agar

see lab book:

Exercise 4-5, page 217
+ = red colonies, red agar
- = colorless colonies

Streptococcus agalactiae

Eosin Methylene
Blue Agar
Hektoen Enteric Agar

Staphylococcus epidermidis

6.5% NaCl broth

Staphylococcus aureus

P disc

Serratia marcescens

A disc

Salmonella typhimurium

Bile Esculin

Proteus vulgaris

Oxidase

+ = bubbles
- = no bubbles
+ = purple
- = no color change
+ = black
- = no color change
+ = zone of inhibition
- = no inhibition
+ = zone of inhibition
- = no inhibition
+ = growth
- = no growth
+ = dark w/ colorless edges
- = colorless; E. coli = green

Proteus mirabilis

Phenylethyl Alcohol
Agar
Catalase

Micrococcus roseus

Mannitol Salt Agar

= clear zone; = green

zone; = no change
+ salt tolerance = growth
+ mannitol ferment. = yellow
+ = good growth
- = poor to no growth

Micrococcus luteus

Phenylalanine
Deaminase
Blood Agar

Klebsiella pneumoniae

Ornithine
Decarboxylase
DNase Agar

Escherichia coli

Lysine Decarboxylase

Enterococcus faecalis

Kligler Iron Agar