Republic of the Philippines

Department of Education
Region XI
DIVISION OF TAGUM CITY
Tagum City National High School
Third Periodical Examination
BIOTECHNOLOGY
Name:_____________________________________________Section: __________Score: ________________
I. Multiple Choice. Read each item carefully. Choices are provided at the left side before the number of each question.
Shade the circle of the letter that corresponds to your answer.
6. What process allows for the
1. What does GMO stand for?
A

A. Genetically modified organism

B. Grand money order
C. Good marketing option
D. Genuine motivation only
2. The scientist used restriction
A

enzymes for what purpose in the experiment?

A. To make the plasmid small enough to transform cells
B. To make cuts in the plasmid DNA
C. To make the plasmid enter the cells
D. To enable the fragments of DNA to form covalent
bonds
3. What is the function of a
A

vector in genetic engineering?

A. cut DNA into many fragments
B. carry DNA into a new cell
C. link together newly joined fragments of DNA
D. make millions of copies of a specific segment of
DNA
4. What is the function of DNA
A

very specific isolation and movement of genes for

desirable traits?
A. selective breeding
B. genetic engineering
C. cloning
D. microinjection
7. What is the most logical
A

sequence of steps for splicing foreign DNA into a

plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes.
III. Extract plasmid DNA from bacterial cells.
IV. Insert the foreign DNA fragments to the plasmid
DNA.
V. Use ligase to seal plasmid DNA to nonplasmid DNA.
A. I, II, IV, III, V
C. III, II, IV, V, I

B. II, III, V, IV, I

D. III, IV, V, I, II
8. Which of the following

produces multiple identical copies of a gene for basic

research or for large scale production of a gene product?
A) restriction enzymes
B) gene cloning
C) DNA ligase
D) gel electrophoresis
9. Which of the following seals

5. Which best describes a

B

ligase in recombinant technology?

A. make millions of copies of a specific segment of
DNA
B. carry DNA into a new cell
C. cut DNA into many fragments
D. link together newly joined fragments of DNA
A

transgenic organism?
A. one that acts as the donor for DNA to be moved into
another organism
B. one produced by cloning a mutant cell
C. one produced by the polymerase chain reaction
D. any genetically modified organism resulting from
laboratory research

the sticky ends of restriction fragments to make

recombinant DNA?
A) restriction enzymes
B) gene cloning
C) DNA ligase
D) gel electrophoresis
10. Which of the following
A

cuts DNA molecules at specific locations?

A) restriction enzymes
B) gene cloning
C) DNA ligase
D) gel electrophoresis
11. Which of the following
A

separates molecules by movement due to size and

electrical charge?
A) restriction enzymes
B) gene cloning
C) DNA ligase
D) gel electrophoresis

12. Restriction fragments of

A

DNA are typically separated from one another by which

process? A) filtering
B)
centrifugation
C) gel electrophoresis
D) PCR
13. In 1997, Dolly the sheep
A

was cloned. Which of the following processes was used?

A) use of mitochondrial DNA from adult female cells of
another ewe
B) replication and dedifferentiation of adult stem cells
from sheep bone marrow
C) separation of an early stage sheep blastula into
separate cells, one of which was incubated in a surrogate
ewe
D) fusion of an adult cell's nucleus with an enucleated
sheep egg, followed by incubation in a surrogate
14. Genetic engineering is
A

being used by the pharmaceutical industry. Which of the

following
is not currently one of the uses?
A) production of human insulin
B) production of human growth hormone
C) creation of products that will remove poisons from
the human body
D) genetic modification of plants to produce vaccines
15.
A

Genetically

engineered

plants
A) are more difficult to engineer than animals.
B) include a transgenic rice plant that can help prevent
vitamin A deficiency.
C) are being rapidly developed, but traditional plant
breeding programs are still the only
method used to develop new plants.
D) are banned throughout the world.
16. Which of the following
A

tools
of
recombinant
DNA
technology
is
INCORRECTLY paired with its use?
A) electrophoresisseparation of DNA fragments
B) DNA ligaseenzyme that cuts DNA, creating the
sticky ends of restriction fragments
C) DNA polymeraseused in a polymerase chain
reaction to amplify sections of DNA
D) restriction enzymeproduction of RFLPs
17. Why are plants more
A

readily manipulated by genetic engineering than

animals?
A) plant genes do not contain introns.
B) more vectors are available for transferring
recombinant DNA into plant cells.
C) a somatic plant cell can often give rise to a complete
plant.

D) genes can be inserted into plant cells by

microinjection.
18.
A

paleontologist

has

recovered a bit of tissue from the 400-year-old preserved

skin of an
extinct dodo (a bird). The researcher would like to
compare a specific region of the DNA from the sample
with DNA from living birds. Which of the following
would be most useful for increasing the amount of dodo
DNA available for testing?
A) RFLP analysis
B) polymerase chain reaction (PCR)
C) electroporation
D) gel electrophoresis
19.
A

In

recombinant

DNA

methods, which statement refers to the term vector?

A) the enzyme that cuts DNA into restriction fragments.
B) the sticky end of a DNA fragment.
C) a RFLP marker.
D) a plasmid used to transfer DNA into a living cell.

20. What are

A

the current benefits of having foods made from

genetically modified crops?
A. They improve farm profitability and make some
farmers' jobs easier.
B. They allow farmers to greatly increase the amount of
crops produced.
C. They improve convenience for consumers, e.g. by
creating foods with longer shelf lives.
D. They improve the nutritional quality of foods.
E. They cause less damage to the environment than
conventional chemical-intensive agriculture.
21. Which of the following is
A

TRUE regarding most currently available genetically

engineered ("GE") plants?
A. Only a few commercially available processed foods
contain GE plants.
B. There has been worldwide acceptance of their use.
C. There are serious biological and environmental issues
regarding their use.
D. Most GE plants are designed to have better flavor and
be more nutritious.
22. Which enzymes are used to
A

cut large segments of DNA into fragments for DNA

fingerprinting?
A. reverse transcriptase
B. DNA polymerase
C. DNA ligase
D. restriction enzymes

23. Why is it more difficult to

A

create transgenic animals than transgenic plants?

A. Animal cells cannot transcribe and translate foreign
DNA.
B. Plants and animals use a different genetic code.
C. It is more difficult to introduce foreign DNA into
animal cells.
D. Animal cells cannot replicate foreign DNA.
24.
A

Gel

electrophoresis

separates DNA fragments according to their ________.

A. base sequence
B. size
C. percentage of nucleotides
D. electrical charge
25.
A

Why

are

bacteria

considered as the "workhorses" of genetic engineering?

A. are always homozygous
B. can readily take up plasmids containing human genes
and then produce the human proteins encoded by those
genes
C. they provide the polymerase for the polymerase chain
reaction
D. reproduce very slowly and accurately
26.
A

Bacteria

have

been

genetically modified for each of the following purposes

EXCEPT ________.
A. to produce bacteria that can clean up toxic wastes
such as oil spills
B. to produce human hormones including insulin and
erythropoietin
C. to produce enzymes used for industrial purposes
D. to produce antibiotics that can be used to treat
bacterial infections
27.
A

In

recombinant

DNA

technology, plasmids may be used to ________.

A. cut DNA at a specific location
B. introduce foreign DNA into human cells
C. activate restriction enzymes
D. introduce foreign DNA into bacteria
28. Which of the following
A

techniques is similar to a molecular "copy machine" for

DNA?
A. gel electrophoresis
B. polymerase chain reaction (PCR)
C. DNA sequencing
D. restriction enzymes
29.
A

Which

plasmids?
A. Plasmids are transgenic bacteria

best

describes

B. Plasmids are enzymes used to cut DNA at specific

locations.
C. Plasmids are circular pieces of DNA that can be used
to carry foreign DNA into cells such as bacteria.
d. Plasmids are clones of bacteria or DNA segments.
30. It is the cells derived from
A

the embryos that develop from eggs that have been

fertilized?
A. Embryonic stem cells
B. stem cells
C. Zygote
D. pluripotent cells
31. Which of the following
A

refers to selective breeding technique?

A.Is a post transcriptional modification in which a single
gene can code for multiple proteins.
B. Is a process to change the genetic makeup of cells by
transfer of genes of interest to another.
C. The process of producing similar populations of
genetically identical individuals.
D. The process by which humans use to selectively
develop particular phenotypic traits.
32. Which of the following
A

refers to an organism that have been genetically

engineered to carry one or more foreign genes?
A. ligated organism
B. transgenic organism
C. homogeneous organism D. mutant
33. The dividing and
A

undifferentiated cells are known as _____________

A. Embryo
B. Proembryo
C. Callus
D. Primordium
34. Aims of plant breeding are
A

to produce ______________
A. early maturing varieties
C. high-yielding varieties

B. disease-free varieties
D. all the above

35. What is meant by cloning?

A

A. Production of HGH gene in E. coli.

B. To preserve the genotype of organism
C. To replace the original one
D. all of these
36. What happens during
A

transformation?
A. a prokaryote is changed into a eukaryote.
B. a cell takes in DNA from outside the cell.
C. foreign DNA is inserted into a plasmid.
D. the chromosome of a bacterium is mutated.

37. Why are plasmids so

A

44. What is joined together in

widely used in recombinant DNA studies?

A. because it is difficult to insert new genes into them
B. because they can be used to transform bacteria
C. because they naturally contain much foreign DNA
D. because they cannot be cut with restriction enzymes

order to produce a recombinant DNA molecule?

A. one mRNA with a DNA segment
B. one mRNA with a tRNA segment
C. two mRNA molecules
D. Two DNA segments

38. Why is inserting plasmids

A

45. It refers to a gene produced

in yeast more complex than inserting them into bacteria?

A. Yeasts are prokaryotes.
B. Yeasts are hybrids.
C. Yeasts are eukaryotes.
D. Yeasts are inbred.
39. Which of the following is a
A

pair of transgenic organisms?

A. a hybridized peach tree and bacteria that make human
insulin
B. a Bt corn plant and a polyploid banana tree
C. a hybridized peach tree and a polyploid banana tree
D. bacteria that make human insulin and a Bt corn plant

for recombinant DNA technology contains a gene from

one organism joined to the regulatory sequence of
another gene.
A. transgene
B. junk gene
C. chimeric gene
D. None
46. Which refers to the group
A

of genetically similar organisms obtained by a sexual

reproduction?
A. Clone
B. Population
C. Assembly
D. None

40. Dolly is a sheep produced

A

47. Which of the following

by cloning. Which of the following is a difference

between Dolly and animals produced by sexual
reproduction?
A. The source of Dollys DNA is a single cell taken from
an adult individual.
B. The DNA molecules in each of Dollys cells are
identical.
C. Dolly has a mix of genes from her foster mother and
the sheep that donated a nucleus of one of its cells.
D. Dolly is genetically identical to her offspring.
41. GM crops that produce a
A

higher yield per plant than unmodified crops would

__________________.
A. produce more food for the same acreage.
B. produce less food for the same acreage.
C. produce more food but would use more land.
D. produce less food and require more land.
42. Which of the following
A
B
C D
produces multiple identical copies of a gene for basic
research or for large-scale production of a gene product?
A. restriction enzymes
B. gene cloning
C. DNA ligase
D. gel electrophoresis
43. Which is true of genetically
A

engineered plants?
A. are more difficult to engineer than animals.
B. include a transgenic rice plant that can help prevent
vitamin A deficiency.
C. are being rapidly developed, but traditional plant
breeding programs are still the only method used to
develop new plants.
D. are banned throughout the world.

describes the group of organisms produced by cloning?

A. asexual method and genetically similar
B. asexual method and genetically dissimilar
C. sexual method and genetically similar
D. sexual method and genetically dissimilar
48.
A

What

are

transgenic

organisms?
A. produced by recombinant DNA technology
B. extinct organisms
C. naturally occurring and endemic
D. produced by traditional plant breeding technique
49. What are the main tools
A

required for recombinant DNA technology?

A. vector, desired gene
B. vector, desired gene, mRNA of desired gene, host,
restriction enzymes, ligases
C. desired gene, host, vector
D. vector, desired gene, mRNA of desired gene, host
50.
A

What

is

involved

in

construction of a recombinant DNA?

A. cleaving DNA with restriction endonuclease and
joining with ligase
B. cleaving DNA with ligase and joining with
endonuclease
C. cleaving and joining DNA with restriction
endonuclease
D. cleaving DNA with restriction endonuclease and
joining with polymerase

51. Which of the following

A

refers to the process of identifying genes of interest by

observing the physical characteristics to find organisms
with interesting or useful characteristics?
A. reverse genetics
B. forward genetics
C. selective breeding
D. cloning
52. Which of the following
A

refers to the process of identifying genes of interest by

disrupting the gene of interest and then screen the
resulting modified organisms for changes in the physical
characteristics?
A. reverse genetics
B. forward genetics
C. selective breeding
D. cloning
53. What is the method of
A

sorting DNA fragments according to its size?

A. electroporation
B. restriction analysis
C. electrophoresis
D. DNA probe
54. Which of the following
A

refers to the genes that regulate when the gene is

activated or inactivated?
A. promoter
B. controller
C. marker
D. vectors
55.
A

When

the

nucleus

is

removed in an egg cell, the result

______________.
A. enucleated oocyte
B. zygote
C. embryo
D. oocyte

is

called

56. Which refers to the method

A

of obtaining new plants from specialized plant parts by

merely pulling the plant parts apart?
A. separation
B. division
C. cutting
D. grafting
57.
A

What method of plant

cloning makes use of plant material from two separate

plants joined together to form one plant?
A. separation
B. division
C. cutting
D. grafting
58. What method of plant
A

cloning makes use a small amount of tissue removed

from a parental plant to regenerate a new plant?
A. grafting
B. tissue culture
C. layering
D. cuttings
59. Which of the following statements about plant
cloning is true?

A. Plant cloning is one of the newest forms of

biotechnology.
B. Cloning plants are much harder than cloning animals.
C. Cuttings are usually only taken from leaves.
D. Plants that have seedless fruit must be reproduced
through vegetative propagation.
60. Which of the following
A

statements about animal cloning is FALSE?

A. Clones can never occur naturally.
B. The main challenge for scientists at this point is to
make the cloning process easier and more efficient.
C. Cloning can produce completely normal animals.
D. One of the problems with selective breeding is that
unfavorable characteristics can be passed on to offspring
along with the favorable characteristics.