PRETEST IN BIOTECHNOLOGY

DIRECTIONS: Read and analyze each item carefully. Write the letter of the correct answer on
the answer sheet.
1. The table below enumerates the parts that are present or absent in three (3) kinds of cell.
Structure Cell A Cell B Cell C
Cell Wall X ✓ ✓
Cell membrane ✓ ✓ ✓
Chloroplast X ✓ X
Centriole ✓ X X
Nucleus ✓ ✓ X
Plasmid X X ✓
Which do you think is the animal cell?
A. Cell A
B. Cell B
C. Cell C
D. Cell A and Cell B
For item no. 2. Study the images below and answer the question that follows.

Illustrated by:
KRMacababbad

Animal Cell Plant Cell Bacterial Cell

2. How do the structure of bacterial cell, different from animal and plant cells?
A. Bacterial cells are generally much bigger than plant & animal cells.
B. Bacteria are of different shapes while plant and animal cells are round.
C. Bacterial cell has small nucleus while both animal and plant cells have bigger distinct
nuclei.
D. Bacterial cell lacks nucleus, mitochondria, Golgi bodies and other membrane bound
organelles which are present in animal and plant cells.

3. Why active transport uses energy?

A. Because the cell transports water.
B. Because the cell moves down a concentration gradient.
C. Because the cell moves against a concentration gradient.
D. Because the cell transports soluble molecules like lipids and carbon dioxide

For item no. 4., Refer to the figure below.

Illustrated by:
KRMacababbad

a b c d e f g
4. Which of the following is the correct arrangement of the phases of cell cycle?
A. ABDCEGF
B. ABDCEFG
C. BADCEFG
D. BADCEGF

5. Which of the following laboratory apparatuses are used to measure volume of liquids?
A. Beaker, graduated cylinder, Erlenmeyer flask, pipette, volumetric flask, burette
B. Test tube, Bunsen Burner, ring clamp, ring stand, wire gauze
C. Thermometer, weighing scale, triple beam balance
D. Florence flask, test tube, dropper, funnel

6. Which of the following BEST describes gene therapy?

A. The removal of the affected person’s genotype to treat a genetic disease.
B. The manipulation of the affected person’s gene to treat a genetic disease.
C. The alteration of the affected person’s genotype to treat a genetic disease.
D. The alteration of the affected person’s phenotype to treat a genetic disease.

7. Which of the following is NOT technique used in biotechnology?

A. Recombinant DNA vaccines
B. Plant Tissue Culture
C. DNA Cloning
D. Gene therapy

8. Which type of microorganism is used for beer brewing?

A. Virus
B. Bacteria
C. Fungi (yeast)
D. Fungi (molds)

9. Which of the following BEST differentiates traditional biotechnology from modern

biotechnology?
A.
Traditional: Uses natural organisms to
modify food and other products.
Modern: Involves manipulation of genes
and living tissues
B.
Traditional: Involves manipulation of
genes to generate new tissue
Modern: Old way to use living
organisms to yield products.
C.
Traditional: old way of manipulating
genes in a controlled environment.
Modern: Involves manipulation of genes
and living tissues
 D.
Traditional: DNA cloning, Tissue
Engineering, Genome Analysis.
Modern: Tissue culture and natural way
of breeding animals.

10. Which of the following microbes has INCORRECT usage?

A. Escherichia coli: used to produce penicillin antibiotics.
B. Pseudomonas: specie with species high bioremediation potential.
C. Acetobacter: converts ethanol to acetic acid in the presence of oxygen.
D. Bacillus subtilis: most frequently used bacterial hosts to produce recombinant proteins.

11. Which of the following BEST illustrates the flow of genetic information, from DNA to RNA
to make a functional product, protein?

A.
Protein Synthesis
Translation Transcription
DNA RNA Protein

B. Replication
Transcription Translation
DNA RNA Protein

C.
Replication
Transcription Translation
RNA DNA Protein

D. Protein Synthesis
Transcription Translation
DNA RNA Protein

12. Which of the following BEST explains this scenario: The dog that was cloned had the same
exact DNA but different color fur than the mother?

A. Cloning can alter one’s characteristics.

B. It is natural for dogs to have different color of fur.
C. Environment plays a huge part in the way organisms develop.
D. When cloning organisms, only one trait is inherited form the parent organism.
13. Which of the following is TRUE about DNA mutations?
A. Mutations contribute to genetic uniformity within species.
B. Mutations can never be inherited even if they have a positive effect.
C. Mutations only occur during DNA synthesis and if errors are made and not corrected in
time.
D. Mutation is the change that occurs in DNA sequence, due to mistakes in copying of DNA
or as result of environmental factors.

For items no. 14-15. Select from the options below:

A. Molecular scissor
B. Gel Electrophoresis
C. Polymerase Chain Reaction
D. Enzyme immunoassay (EIA) kit

14. Which tool is NOT being used in genetic engineering? ________

15. What technique in genetic engineering is shown in the image?

Illustrated by:
KRMacababbad

16. In what way is genetic engineering of crops similar to the more traditional practice of
artificial selection of crop varieties?
A. Both methods result in a crop that have some desirable characteristic.
B. Both techniques produce plants that have higher yields than previously.
C. In both cases, two different crop plants are crossed to develop a new one.
D. In both procedures, genes from unrelated organisms can be added to the plant.

17. Which steps of Recombinant DNA technology is arranged in sequence?

A.
1. Ligation of DNA Molecule
2. Digestion of Restriction Enzymes
3. Isolation of Recombinant Cell
4. Isolation of Genetic Material
5. Amplification through PCR
6. Obtaining or culturing the Foreign Gene product
7. Insertion of Recombinant DNA into Host
B.
1. Isolation of Genetic Material
2. Digestion of Restriction Enzymes
3. Amplification through PCR
4. Ligation of DNA Molecule
5. Insertion of Recombinant DNA into Host
6. Isolation of Recombinant Cell
7. Obtaining or culturing the Foreign Gene product

C.
1. Digestion of Restriction Enzymes
2. Isolation of Genetic Material
3. Amplification through PCR
4. Ligation of DNA Molecule
5. Insertion of Recombinant DNA into Host
6. Isolation of Recombinant Cell
7. Obtaining or culturing the Foreign Gene product

D.
1. Digestion of Restriction Enzymes
2. Isolation of Genetic Material
3. Amplification through PCR
4. Ligation of DNA Molecule
5. Insertion of Recombinant DNA into Host
6. Obtaining or culturing the Foreign Gene product
7. Isolation of Recombinant Cell

18. Which of the following is TRUE about Recombinant DNA technology?

A. The final step in Recombinant DNA technology is isolation of desired DNA.
B. Recombinant DNA is the removal of two different strands of DNA.
C. DNA Ligase separates a cut fragment of DNA and the vector
D. BT corn is a product of Recombinant DNA Technology.

19. Which shows the beneficial use of biotechnology to industry?

A. By using of cells to synthesize chemicals
B. By using different organisms to treat diseases.
C. Production of equipment and machines which supports DNA research.
D. Utilization of chemicals for the design and manufacture of new plastics and textiles.

20. What biotechnology-based diagnostic tools or products are used in human health care?
A. Fermentation
B. Bioremediation
C. Covid -19 vaccines
D. DNA fingerprinting
21. Why is biotechnology important in agriculture?
A. To reduce pots-harvest loses.
B. To decrease nutritional quality of crops.
C. To produce plants susceptible to diseases.
D. To increased levels of toxins in food crops.

22. Which from the images BEST illustrates the use of biotechnology in environmental
protection?

A. C.

Illustrated by: Krmacababbad

B. Open learn.com
D.

Illustrated by: Krmacababbad

23. What do you think is the main purpose why scientist removed a gene from a scorpion tails
and inserted into cabbage to produce a transgenic plant known as venomous cabbage?
A. Limit pesticide use while still preventing insects from damaging crops.
B. To incorporate toxins harmful to organisms when consuming the crop.
C. For the plant to create antibodies to fight diseases
D. To produce vaccines out from the cabbage.

24. What is the aim of DNA manipulation?

A. produce synthetic fertilizers.
B. reduce genetic contamination.
C. decrease resistance of plants to certain pests or disease.
D. produce disease-and insect resistant crops, edible vaccines, larger crops

25. Which of the following is NOT a reason why we use animals in biotechnological researches?
A. Animals are biologically similar to humans.
B. Animals are susceptible to many of the same health problems, and they have short life-
cycles so they can easily be studied throughout their whole life-span.
C. Scientists cannot easily control the environment around the animal like diet, temperature,
lighting, etc., which would be easier to do with people.
D. It is unethical to purposely expose human beings to health risks in order to observe the
course of a disease.
ANSWER KEYS
1. A
2. D
3. C
4. D
5. A
6. C
7. A
8. C
9. A
10. A
11. B
12. C
13. D
14. D
15. A
16. A
17. B
18. D
19. D
20. C
21. A
22. B
23. A
24. D
25. C

Prepared by:

KRSICHELLE R. MACABABBAD
Teacher I