lsozymes of 23 cultures of the anaerobic rumen fungi and seven cultures of aerobic chytridiomycete fungi were analysed by PAGE. A total of 14 isozyme loci were successfully typed by PAGE. They were peptidase A & C-I, peptidase A & C-2,...
morelsozymes of 23 cultures of the anaerobic rumen fungi and seven cultures of
aerobic chytridiomycete fungi were analysed by PAGE. A total of 14 isozyme
loci were successfully typed by PAGE. They were peptidase A & C-I, peptidase
A & C-2, peptidase D-I, peptidase D-2, malate dehydrogenase-I, malate
dehydrogenase-2, esterase-I, esterase-2, malic enzyme-I, malic enzyme-2,
isocitrate dehydrogenase, shikimate dehydrogenase, phosphoglucomutase and
6-phosphogluconate dehydrogenase. lsozyme analysis can be used for
studying the genetic relationships among the different anaerobic rumen fungi
and the aerobic chytridiomycete fungi and the isozyme characteristics can
serve as additional taxonomic criteria in the classification of the anaerobic
rumen fungi. A dendrogram based on the isozyme data demonstrated that the
anaerobic rumen fungi formed a cluster, indicating a monophyletic group,
distinctly separated from the aerobic chytridiomycete fungi. Piromyces
communis and P. minutus showed a close relationship but P. spiralis showed a
more distant relationship to both P. communis and P. minutus. Piromyces as a
whole was more related to Caecomyces than to Neocallimastix. Orpinomyces
was also found to be more related to Piromyces and Caecomyces than to
Neocallimastix. Orpinomyces intercalaris C 70 from cattle showed large genetic
variation from 0. joyonii, indicating that it is a different species.
